Transmission light imaging receiving device and laser confocal microscope
By introducing a transmitted light imaging receiver into a laser confocal microscope, the problem of traditional microscopes lacking transmitted light imaging has been solved, realizing the transmitted light imaging function of the laser confocal microscope, simplifying the operation process, improving data accuracy, and reducing costs.
Patent Information
- Application Number
- CN202423174115.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-12-30
- Estimated Expiration
- 2034-12-20
AI Technical Summary
Traditional laser confocal microscopes lack transmitted light imaging capabilities, making it difficult for researchers to obtain differential interference images of samples.
A transmissive light imaging receiver is designed, including a housing, a detection component, and a lens module. By setting a first through hole and a second through hole in the housing to form an optical channel, an optical path that does not interfere with each other is realized. Combined with the movable installation of the lens module and the use of a cover plate, selective switching and protection of the optical path are ensured.
It realizes the transmission light imaging function of laser confocal microscope, simplifies the operation process, shortens the observation time, improves the accuracy and authenticity of measurement data, and reduces costs and maintenance difficulty.
Smart Images
Figure CN223742851U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of microscope technology, and in particular to a transmitted light imaging receiving device and a laser confocal microscope. Background Technology
[0002] A laser confocal microscope is a high-resolution optical instrument that uses a laser as its light source and performs precise, point-by-point, line-by-line scanning of the sample through rapid rotation of a galvanometer. This microscope utilizes a small aperture to achieve an optical sectioning effect, thereby obtaining clear images of different depth layers of the sample. The scanning head, a key component of the laser confocal microscope, is typically located at the optical path interface on the left or rear side of the microscope. It integrates key components such as the laser source, galvanometer, and photodetector. The scanning head's task is to ensure the laser beam is accurately focused on the sample surface and to efficiently collect the fluorescence signal emitted by the sample.
[0003] Transmitted light imaging technology is widely used for observing the morphology and structure of samples. Especially when a microscope is equipped with a polarizer, analyzer, and prism, it can achieve differential interference contrast (DIC) imaging under transmitted light. This imaging method can give cell images a three-dimensional relief effect, greatly facilitating researchers' observation of the depth features of cell structures. However, traditional laser confocal microscopes, because their laser source and photodetector are integrated into the scanning head, mainly achieve reflected light imaging and lack the ability to perform transmitted light differential interference imaging. This poses a certain inconvenience for researchers who need to acquire differential interference images of samples. Utility Model Content
[0004] Since existing laser confocal microscopes lack transmitted light imaging capabilities, it is necessary to provide a transmitted light imaging receiver and a laser confocal microscope.
[0005] A transmitted light imaging receiver for laser confocal microscopy includes:
[0006] The outer casing has a first through hole and a second through hole arranged opposite to each other to form a light channel. The first through hole is used to connect to the illumination element of the laser confocal microscope, and the second through hole is used to connect to the microscope body of the laser confocal microscope.
[0007] A detection component, fixedly connected to the housing and having a receiving target surface for receiving light; and
[0008] A lens module includes a lens barrel having a light-inlet surface and a light-outlet surface and being movably mounted in the housing, and a reflector located on the light-inlet surface and fixed to the lens barrel;
[0009] When the lens module is located in the light channel, the light exit surface corresponds to the receiving target surface and the mirror corresponds to the second through hole for the receiving target surface to receive sample transmitted light from the microscope body; when the lens module is located outside the light channel, the light paths of the first through hole and the second through hole are communicated.
[0010] In this way, two light paths that do not interfere with each other are realized inside the transmitted light imaging receiving device. An experimenter can observe a sample by selecting different light paths without using two different types of microscopes, saving necessary process steps of instrument operation, shortening observation time, and facilitating experimenter operation. Since the sample does not need to be moved during the entire observation experiment, it is not easily disturbed by external environmental factors, ensuring the accuracy and authenticity of measurement data.
[0011] In one of the embodiments, the transmitted light imaging receiving device further comprises a guide rail fixedly arranged in the housing, a sliding seat slidably mounted on the guide rail, and a first driving member drivingly connected to the sliding seat, and the lens barrel is fixedly connected to the sliding seat.
[0012] In this way, the lens module slides along the guide rail without shaking or deviating, which is beneficial to increase the stability of the lens module sliding and the accuracy of the position.
[0013] In one of the embodiments, the sliding seat comprises a sliding block and a base detachably mounted on the sliding block.
[0014] In this way, the sliding block can be matched with the guide rail to form a combined standard part without the need for redesign, which is beneficial to reduce the production cost of the transmitted light imaging receiving device.
[0015] In one of the embodiments, the transmitted light imaging receiving device further comprises a first cover plate fixedly arranged on the base, and when the lens module is located outside the light channel, the first cover plate covers the receiving target surface of the detection assembly.
[0016] In this way, it is beneficial to prevent stray light from entering the receiving target surface, thereby protecting the detection assembly.
[0017] In one of the embodiments, the transmitted light imaging receiving device further comprises a second cover plate fixedly connected to the base, and when the lens module is located in the light channel, the second cover plate covers the first through hole.
[0018] In this way, it is beneficial to prevent the strong light of the illuminating member from interfering with the detection assembly, and also beneficial to protect the detection assembly and improve the service life of the detection assembly.
[0019] In one of the embodiments, the second cover plate has a light shielding surface abutting against the shell near a side of the first through hole.
[0020] In this way, the light shielding effect of the second cover on the first through hole is improved.
[0021] In one of the embodiments, the detection assembly includes a signal amplification body and a detection body providing the receiving target surface, the signal amplification body includes an amplifier mounting seat fixedly connected to the shell and an amplifier body electrically connected to the detection body and mounted on the amplifier mounting seat, and the amplifier mounting seat is in a U shape.
[0022] In this way, the U-shaped amplifier mounting seat helps to reduce the weight of the entire transmitted light imaging receiving device and stably supports the amplifier body.
[0023] In one of the embodiments, the shell includes a fixed plate providing the first through hole and a protective cover covering the fixed plate and providing the second through hole, and the detection assembly is fixedly connected to the fixed plate.
[0024] The transmitted light imaging receiving device further includes an aviation socket fixedly arranged on the fixed plate, and the detection assembly is electrically connected to the aviation socket.
[0025] In this way, the reliability of the electrical connection in the transmitted light imaging receiving device is improved, the power supply and external signal transmission of the detection assembly are realized through the aviation socket, and only the replacement is needed during maintenance, thereby reducing the maintenance difficulty.
[0026] The application further provides a laser confocal microscope, which includes:
[0027] An illuminating member has a light emitting end;
[0028] A scanning head has a laser emitting port;
[0029] A microscope body has a first optical path interface corresponding to the light emitting end and a second optical path interface connected to the laser emitting port; and
[0030] The transmitted light imaging receiving device as described above is located between the illuminating member and the microscope body, the first through hole of the transmitted light imaging receiving device is communicated with the light emitting end, and the second through hole of the transmitted light imaging receiving device is communicated with the first optical path interface.
[0031] With this setup, the transmitted light imaging receiver is connected to the illumination optical path of the microscope body through two optical path interfaces, enabling the laser confocal microscope to also have the function of transmitted light imaging. It also shares the existing illumination optical path of the microscope body, eliminating the need to adjust the existing main structure of the microscope body. The installation method is simple and easy to understand, saving labor and material costs.
[0032] In one embodiment, the microscope body includes a frame body with a main optical path, a transmitted light differential interference imaging component, and a second driving component. The first optical path interface and the second optical path interface are respectively located at both ends of the main optical path. The second driving component is connected to the transmitted light differential interference imaging component to switch the transmitted light differential interference imaging component between accessing the main optical path and exiting the main optical path.
[0033] This configuration, by adding a second driving component and a transmitted light differential interference imaging component, allows the laser confocal microscope to be easily switched to transmitted light differential interference imaging, simplifying the operation for experimental personnel. Attached Figure Description
[0034] Figure 1 A side view of a transmission light imaging receiving device in one embodiment provided in this application;
[0035] Figure 2 for Figure 1 A front view of the transmitted light imaging receiver shown;
[0036] Figure 3 for Figure 1 The diagram shows a partial structure of the transmitted light imaging receiver in its first state.
[0037] Figure 4 for Figure 3 A schematic diagram of part of the structure of the transmitted light imaging receiver shown in the second state.
[0038] Figure 5 for Figure 4 A schematic diagram of the transmitted light imaging receiver shown from another perspective;
[0039] Figure 6 This application provides a schematic diagram of the structure of a laser confocal microscope.
[0040] Figure 7 An optical path diagram of a laser confocal microscope provided in one embodiment of the application.
[0041] Figure label:
[0042] 100, transmission light imaging receiving device; 101, first through hole; 102, second through hole; 103, light channel; 1, fixed plate; 2, aviation socket; 3, first driving piece; 4, guide rail; 5, amplifier mounting seat; 6, amplifier body; 7, detector body; 8, detector mounting seat; 9, first cover plate; 10, lens barrel; 11, base; 12, condenser; 13, mirror; 14, second cover plate; 15, first connecting seat; 16, second connecting seat; 17, protective cover; 18, sliding block; 200, illuminating part; 300, microscope main body; 301, polarizer; 302, differential interference contrast prism; 303, sample; 304, analyzer; 305, second driving piece; 400, scanning head; 401, laser light source; 402, dichroic mirror; 403, pinhole; 404, reflected light detector; 405, pinhole lens; 406, galvanometer; 407, scanning lens; 500, computer. DETAILED DESCRIPTION
[0043] In order to make the above objectives, characteristics and advantages of the present application more apparent, specific embodiments of the present application will be described in detail below with reference to the drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. It will be apparent, however, to one skilled in the art that the present application can be practiced in a variety of ways beyond the specific embodiments described herein without departing from the spirit of the present application, and it is intended that the present application cover all such variations as fall within the scope of the appended claims. It is also to be understood that features of the foregoing detailed description and / or accompanying drawings, which do not necessitate novelty, can also be implemented and combined in any manner by one skilled in the art.
[0044] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.
[0045] In addition, the terms "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically limited.
[0046] In the utility model, unless otherwise expressly provided and limited, the terms "mounting", "connection", "connecting", "fixing" and the like should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrated; it can be mechanical connection, or electrical connection; it can be direct connection, or indirect connection through an intermediate medium, it can be the internal communication of two elements or the interaction relationship of two elements, unless otherwise expressly limited. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.
[0047] In the utility model, unless otherwise expressly provided and limited, the first feature is "on" or "under" the second feature, which can be direct contact of the first and second features, or indirect contact of the first and second features through an intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.
[0048] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on another element or there can be a middle element. When an element is considered to be "connected" to another element, it can be directly connected to another element or there can be a middle element. The terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used herein are for illustrative purposes only and are not the only embodiment.
[0049] The laser confocal microscope is an optical instrument with high resolution, which uses laser as light source and scans the sample point by point and line by line through the rapid rotation of the galvanometer. The microscope uses a small hole to realize the effect of optical sectioning, so that clear images of different depth levels of the sample can be obtained. The scanning head, as a key component of the laser confocal microscope, is generally arranged at the left or rear side of the microscope light path interface, and the inside is integrated with laser source, galvanometer and photoelectric detector and other key elements. The task of the scanning head is to ensure that the laser beam is accurately focused on the sample surface and efficiently collects the fluorescence signal emitted by the sample.
[0050] Transmission light imaging technology is widely used in sample morphology structure observation. Especially when the microscope is configured with a polarizer, an analyzer and a prism, differential interference contrast (DIC) imaging under transmission light can be realized, which can give the cell image a three-dimensional relief effect, greatly facilitating researchers to observe the depth characteristics of cell structure. However, the traditional laser confocal microscope has the laser light source and the photodetector integrated in the scanning head, mainly realizing reflected light imaging, and does not have the ability of transmission light differential interference imaging, which brings some inconvenience to researchers who need to obtain sample differential interference images.
[0051] Therefore, it is necessary to provide a transmission light imaging receiving device and a laser confocal microscope capable of receiving transmission light imaging.
[0052] Please refer to Figures 1 to 4 , Figure 1 the side view of the transmission light imaging receiving device 100 in an embodiment provided by the present application, Figure 2 for Figure 1 the front view of the transmission light imaging receiving device 100, Figure 3 for Figure 1 the schematic diagram of the partial structure of the transmission light imaging receiving device 100 in the first state, Figure 4 for Figure 3 the schematic diagram of the partial structure of the transmission light imaging receiving device 100 in the second state. The transmission light imaging receiving device 100 provided by the present application comprises a shell, a detection assembly and a lens module, the shell is provided with a first through hole 101 and a second through hole 102 arranged oppositely to form a light channel 103, the first through hole 101 is used for connecting the illuminating part 200 of the laser confocal microscope, and the second through hole 102 is used for connecting the microscope main body 300 of the laser confocal microscope, the detection assembly is fixedly connected in the shell and has a receiving target surface for receiving light, and the lens module comprises a lens barrel 10 and a mirror 13, the lens barrel 10 has an entrance surface and an exit surface and is movably installed in the shell, and the mirror 13 is located at the entrance surface and is fixedly arranged on the lens barrel 10. When the lens module is located in the light channel 103 (as shown in Figure 3 ), the transmission light imaging receiving device 100 is in the first state, the exit surface corresponds to the receiving target surface, and the mirror 13 and the second through hole 102 are used to make the receiving target surface receive the transmission light from the sample 303 of the microscope main body 300, and when the lens module is located outside the light channel 103 (as shown in Figure 4As shown, the transmitted light imaging receiver 100 is in its second state, with the optical paths of the first through-hole 101 and the second through-hole 102 connected. This creates two independent optical paths within the transmitted light imaging receiver 100, allowing researchers to observe the sample 303 by selecting different optical paths without needing two different microscopes. This saves on necessary instrument operation steps, shortens observation time, and simplifies operation. Since the sample 303 does not need to be moved during the entire observation experiment, it is less susceptible to interference from external environmental factors, ensuring the accuracy and authenticity of the measurement data. Optionally, in one embodiment provided in this application, the microscope tube 10 is further provided with a condenser lens 12 to increase the amount of light received transmitted to the receiving target surface. To facilitate the installation of the transmitted light imaging receiver 100, the first through-hole 101 is also provided with a first connecting seat 15 for connecting the illumination element 200, and the second through-hole 102 is also provided with a second connecting seat 16 for connecting the microscope body 300.
[0053] Please see Figure 3 , Figure 4 and Figure 5 , Figure 5 for Figure 4 The diagram shows a structural schematic of the transmitted light imaging receiver 100 from another perspective. Optionally, to increase the smoothness of the lens module's sliding motion, in one embodiment of this application, the transmitted light imaging receiver 100 further includes a guide rail 4 fixed within the housing, a sliding seat slidably mounted on the guide rail 4, and a first driving member 3 driven by the sliding seat. The lens barrel 10 is fixedly connected to the sliding seat. The lens module slides along the guide rail 4 without shaking or shifting, which helps increase the smoothness and positional accuracy of the lens module's sliding motion. Specifically, to reduce assembly difficulty, the sliding seat includes a slider 18 and a base 11 detachably mounted on the slider 18. The slider 18 can be combined with the guide rail 4 to form a standard assembly without redesign, which helps reduce the manufacturing cost of the transmitted light imaging receiver 100. Optionally, in this embodiment, the first driving member 3 is a push rod motor.
[0054] Please see Figure 3 and Figure 4Optionally, in order to prevent stray light from entering the receiving target surface, the transmitted light imaging receiving device 100 further comprises a first cover plate 9 fixed to the base 11, when the lens module is located outside the light channel 103, the first cover plate 9 covers the receiving target surface of the detection assembly, thus facilitating the protection of the detection assembly, thereby prolonging the service life of the detection assembly. In order to avoid the strong light of the illuminating part 200 interfering with the detection assembly, especially when the lens module is located in the light channel 103 and the illuminating part 200 fails to close in time, the light of the illuminating part 200 will enter the transmitted light imaging receiving device 100 through the first through hole 101 and produce stray light, therefore, in an embodiment provided by the present application, the transmitted light imaging receiving device 100 further comprises a second cover plate 14 fixedly connected to the base 11, when the lens module is located outside the light channel 103, the second cover plate 14 covers the first through hole 101, and at the same time, this also facilitates the protection of the detection assembly, thereby prolonging the service life of the detection assembly. Further, the second cover plate 14 has a light shielding surface abutting against one side of the housing close to the first through hole, in this way, the second cover plate 14 is closely attached to the housing, and when covering the first through hole 101, the light shielding effect is better, and the second cover plate 14 specifically has a stepped shape for providing a space for the first driving part.
[0055] Please refer to Figure 3 and Figure 4 again, in order to facilitate assembly, the housing comprises a fixed plate 1 providing the first through hole 101 and a protective cover 17 covering the fixed plate 1 and providing a second through hole 102, the guide rail 4 is fixedly connected to the fixed plate 1, the detection assembly comprises a signal amplification main body and a detection main body, the detection main body specifically comprises a detector mounting seat 8 fixedly connected to the fixed plate 1 and a detector body 7 mounted to the detector mounting seat 8, the receiving target surface is located at the detector body 7, and the signal amplification main body specifically comprises an amplifier mounting seat 5 fixedly connected to the fixed plate 1 and an amplifier body 6 mounted to the amplifier mounting seat 5 and electrically connected to the detection main body, in this way, the detection main body and the amplifier main body are conveniently fixed to the fixed plate 1, and in particular, the amplifier mounting seat 5 has a U shape, which reduces the weight of the transmitted light imaging receiving device 100 and also can stably support the amplifier body 6. The U-shaped amplifier mounting seat 5 comprises a connecting portion fixedly connected to the fixed plate 1 and support portions respectively extending from both ends of the connecting portion away from the fixed plate 1, and the amplifier body 6 is mounted to the support portions, and optionally, the connecting portion, the fixed plate 1, the support portions and the amplifier body 6 are all connected by bolts, which facilitates maintenance and reduces maintenance costs.
[0056] Please refer to Figure 3 and Figure 4Optionally, in an embodiment provided by the present application, the transmitted light imaging receiving device 100 further comprises an aviation socket 2 fixed to the fixing plate 1, the first driving member 3, the detection main body and the signal amplification main body are electrically connected to the aviation socket 2, and the aviation socket 2 is used for externally connecting the computer 500 to export the image data of the detection assembly. In this way, the reliability of the electrical connection in the transmitted light imaging receiving device 100 is increased, the power supply of the detection assembly and the external signal transmission are both realized through the aviation socket 2, and only the replacement is needed during maintenance, thereby reducing the difficulty of maintenance.
[0057] Please refer to Figure 6 , Figure 6 A structural schematic diagram of a laser confocal microscope provided by the present application. The laser confocal microscope comprises an illumination member 200, a microscope main body 300, a scanning head 400 and a transmitted light imaging receiving device 100. The illumination member 200 has a light emitting end, the scanning head 400 has a laser emitting port, the microscope main body 300 has a first light path interface corresponding to the illumination member 200 and a second light path interface connected to the laser emitting port, and the transmitted light imaging receiving device 100 is located between the illumination member 200 and the microscope main body 300. The first through hole 101 of the transmitted light imaging receiving device 100 is communicated with the light emitting end, and the second through hole 102 of the transmitted light imaging receiving device 100 is communicated with the first light path interface. In this way, the transmitted light imaging receiving device 100 is connected into the illumination light path of the microscope main body 300 through the two light path interfaces, so that the laser confocal microscope also has the function of transmitted light imaging, and shares the illumination light path of the existing microscope main body 300. The main structure of the existing microscope main body 300 does not need to be adjusted, the installation mode is simple and easy to understand, and the labor cost and material cost are saved.
[0058] Please refer to Figure 7 , Figure 7An embodiment of the laser confocal microscope provided in the application is shown in the light path diagram. The laser light source 401, the dichroic mirror 402, the pinhole 403, the reflected light detector 404, the pinhole lens 405, the galvanometer 406 and the scanning lens 407 are arranged in sequence along the light path in the scanning head 400. The microscope body 300 comprises a rack body with a main light path, a transmitted light differential interference imaging assembly and a second driving member 305. The second driving member 305 is drivingly connected to the transmitted light differential interference imaging assembly for switching the transmitted light differential interference imaging assembly between access to the main light path and removal from the main light path. By adding the second driving member 305 and the transmitted light differential interference imaging assembly, the laser confocal microscope can also be conveniently switched to transmitted light differential interference imaging, and the operation of the experimental personnel is simplified. The transmitted light differential interference imaging assembly comprises the polarizer 301, the differential interference contrast prism 302 and the analyzer 304 which are coaxially and sequentially arranged. When the transmitted light differential interference imaging assembly accesses the main light path, the polarizer 301 and the differential interference contrast prism 302 are located on the laser receiving surface of the sample 303, and the analyzer 304 is located on the laser transmitting surface of the sample 303.
[0059] Please refer to Figure 6 and Figure 7 Based on the current light path and structure, the laser confocal microscope provided in the application has four use modes: visual mode, ordinary transmitted light imaging mode, laser confocal scanning mode and transmitted light differential interference imaging mode. The light path and structure arrangement in the four modes are as follows:
[0060] When the laser confocal microscope is in the visual mode, the first driving member 3 drives the lens module and makes it located outside the light channel 103. The light emitted by the illuminating member 200 enters the microscope body 300 and reaches the sample 303 to be directly observed by the human eye, which facilitates the rough determination of the observation range of the sample 303.
[0061] When the laser confocal microscope is in the ordinary transmitted light imaging mode, the first driving member 3 drives the lens module and makes it located in the light channel 103, and the second driving member 305 drives the transmitted light differential interference imaging assembly and makes it located outside the main light path. The laser emitted by the scanning head 400 reaches the sample 303 and passes through the sample 303 to generate transmitted light with sample information, and the detection assembly receives the transmitted light for imaging.
[0062] When the laser confocal microscope is in the laser confocal scanning mode, the second driving member 305 drives the transmitted light differential interference imaging assembly and makes it located outside the main light path. The laser emitted by the scanning head 400 reaches the sample 303 and generates reflected light with sample information, and the reflected light detector 404 in the scanning head 400 receives the reflected light for imaging.
[0063] When the laser confocal microscope is in the transmission light differential interference imaging mode, the first driving member 3 drives the lens module and makes it located in the light channel 103, the second driving member 305 drives the transmission light differential interference imaging assembly and makes it located in the main light path, the differential interference image light with sample information is generated after the scanning head 400 emits the differential interference image light through the transmission light differential interference imaging assembly and the sample 303, and the detection assembly receives the differential interference image light for imaging.
[0064] It can be understood that the accuracy of the above four modes increases in turn, which facilitates the experimental personnel to quickly obtain the required sample local image. It is worth noting that the ordinary transmission light imaging mode and the laser confocal scanning mode can be used at the same time, which further shortens the time for the experimental personnel to obtain the required sample local image, and improves the observation efficiency.
[0065] It can be understood that the accuracy of the above four modes increases in turn, which facilitates the experimental personnel to quickly obtain the required sample local image. It is worth noting that the ordinary transmission light imaging mode and the laser confocal scanning mode can be used at the same time, which further shortens the time for the experimental personnel to obtain the required sample local image, and improves the observation efficiency.
[0066] The above-described embodiments only express several implementation manners of the present application, the description is relatively specific and detailed, but it should not be understood as the limitation of the scope of the present application. It should be pointed out that, for the ordinary skilled in the art, on the premise of not departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application patent should be subject to the appended claims.
Claims
1. Transmission light imaging receiving device for laser confocal microscopy, characterized in that, The application relates to a transmission light imaging receiving device, which comprises the following parts: a shell, which is provided with a first through hole and a second through hole arranged oppositely to form a light channel, the first through hole is used for connecting an illuminating part of a laser confocal microscope, and the second through hole is used for connecting a microscope main body of the laser confocal microscope; a detection assembly, which is fixedly connected to the shell and has a receiving target surface for receiving light; a lens module, which comprises a lens barrel having a light inlet surface and a light outlet surface, a mirror fixed to the lens barrel and located at the light inlet surface, and the lens barrel is movably installed in the shell. When the lens module is located in the light channel, the light outlet surface corresponds to the receiving target surface, and the mirror corresponds to the second through hole, so that the receiving target surface receives sample transmission light from the microscope main body; when the lens module is located outside the light channel, the light paths of the first through hole and the second through hole are connected. The transmission light imaging receiving device further comprises a guide rail fixed to the shell, a sliding seat slidably installed on the guide rail and a first driving part drivingly connected to the sliding seat, and the lens barrel is fixedly connected to the sliding seat.
2. The transmissive photoinaging receiver of claim 1 wherein, The sliding seat comprises a sliding block and a base detachably installed on the sliding block.
3. The transmissive photoinaging receiver of claim 2, wherein The transmission light imaging receiving device further comprises a first cover plate fixed to the base, and when the lens module is located outside the light channel, the first cover plate covers the receiving target surface of the detection assembly.
4. The transmissive photoinaging receiver of claim 3 wherein, The transmission light imaging receiving device further comprises a second cover plate fixedly connected to the base, and when the lens module is located in the light channel, the second cover plate covers the first through hole.
5. The transmissive photoinaging receiver of claim 3 wherein, The second cover plate has a light shielding surface abutting against one side of the shell close to the first through hole.
6. The transmissive photoinaging receiver of claim 5 wherein, The detection assembly comprises a signal amplification main body and a detection main body providing the receiving target surface, the signal amplification main body comprises an amplifier mounting seat fixedly connected to the shell and an amplifier body electrically connected to the detection main body and installed on the amplifier mounting seat, and the amplifier mounting seat is in a U shape.
7. The transmissive photoinaging receiver of claim 6 wherein, The shell comprises a fixed plate providing the first through hole and a protective cover covering the fixed plate and providing the second through hole, and the detection assembly is fixedly connected to the fixed plate.
8. The transmissive photoinaging receiver according to any one of claims 1 to 7, characterized in that The transmission light imaging receiving device further comprises an aviation socket fixed to the fixed plate, and the detection assembly is electrically connected to the aviation socket. The application relates to a transmission light imaging receiving device, which comprises the following parts:
9. Laser confocal microscope, characterized in that, an illuminating part, which has a light emitting end; a scanning head, which has a laser emitting port; a microscope main body, which has a first light path interface corresponding to the light emitting end and a second light path interface connected to the laser emitting port; and the transmission light imaging receiving device according to any one of claims 1 to 8, which is located between the illuminating part and the microscope main body, the first through hole of the transmission light imaging receiving device is connected to the light emitting end, and the second through hole of the transmission light imaging receiving device is connected to the first light path interface. 10. The laser scanning confocal microscope according to claim 9, characterized in that The microscope body comprises a rack body with a main light path, a transmission light differential interference imaging assembly and a second driving member, the first light path interface and the second light path interface are respectively located at two ends of the main light path, and the second driving member is drivingly connected to the transmission light differential interference imaging assembly for switching the transmission differential interference imaging assembly between access to the main light path and removal from the main light path.