Multifunctional push-pull rod and circulating tumor cell screening and separating device

Abstract

The utility model provides a multifunctional push-pull rod and a circulating tumor cell screening and separating device, which belong to the technical field of medical detection.The multifunctional push-pull rod comprises a push-pull rod body and an electromagnet, one end of the push-pull rod body is a connecting end, and the electromagnet is mounted at the connecting end; the electromagnet is set to generate a magnetic field when powered on and remove the magnetic field when powered off; the device has the advantages that the electromagnet and the push-pull rod are combined together, so that the whole device is more compact, the function integration level is improved, the on-off of a magnetic field is achieved through the power-on / power-off control of the electromagnet, enrichment and cleaning operation are facilitated, and the electromagnet is located at the connecting end of the push-pull rod body, so that the cleaning efficiency is improved. Therefore, the electromagnet can be located on the center line of the mixing chamber, so that an external magnetic field is uniformly distributed in the mixing chamber, and the enrichment effect of CTCs is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to medical detection technical field relates to a multifunctional push -and -pull rod and circulating tumor cell screening separation device. BACKGROUND

[0002] The technology for detecting circulating tumor cells (CTCs) based on surface-enhanced Raman scattering (SERS) is a detection method for identifying and quantifying CTCs from peripheral blood. The specific steps of this detection method are as follows: 1. Prepare to collect patient blood samples; 2. Mix and incubate the blood sample with SERS active nanomaterials; 3. Use an external magnetic field to enrich CTCs-magnetic bead complexes from the blood; 4. Separate the waste liquid from the enriched CTCs; 5. Use buffer solution for multiple washes to collect CTCs-magnetic bead complexes; 6. Place the collected CTCs-magnetic bead complexes in a Raman spectrometer for detection.

[0003] At present, there is an invention patent with application number CN202010514536.0, named circulating tumor cell screening and separation device and method and application. This device has the functions of realizing steps 2 to 5 described above. Specifically, the device drives the piston to move by setting a piston push rod, and enriches CTCs by using an independent or external magnet. However, the structure of this device is not compact, and the piston push rod and the magnet are arranged separately, resulting in low functional integration and great inconvenience in operation. Moreover, there is room for improvement in the enrichment effect. UTILITY MODEL CONTENTS

[0004] The utility model aims at the above-mentioned problems existing in the prior art, and provides a multifunctional push -and -pull rod and circulating tumor cell screening separation device.

[0005] The utility model can realize the purpose through the following technical scheme: a multifunctional push -and -pull rod, comprising: push -and -pull rod body and electromagnet, the one end of push -and -pull rod body is set as the connecting end, the electromagnet is installed in the connecting end, the electromagnet is set as generating magnetic field when electrifying and removing magnetic field when deenergizing.

[0006] Preferably, the electromagnet is fixedly installed inside the connecting end, and the electromagnet can attract magnetic substances to the outer peripheral surface of the connecting end through the wall part of the connecting end when electrified.

[0007] Preferably, the push-pull rod body is provided with an inner hole, one end of the inner hole extends to the inside of the connecting end, the wall of the push-pull rod body is provided with a wire hole, the wire hole is in communication with the other end of the inner hole, the electromagnet is mounted at one end of the inner hole and located inside the connecting end, and the wire on the electromagnet passes out of the inner hole through the wire hole.

[0008] Preferably, the outer peripheral surface of the connecting end is provided as a smooth surface.

[0009] Preferably, the connecting end of the push-pull rod body is fixedly connected with the center of the piston block.

[0010] Preferably, the connecting end of the push-pull rod body is fixedly connected with the center of the piston block.

[0011] Preferably, the connecting end of the push-pull rod body is fixedly connected with the center of the piston block.

[0012] Preferably, the connecting end of the push-pull rod body is fixedly connected with the center of the piston block.

[0013] Compared with the prior art, the utility model has the advantages that:

[0014] 1. By combining the electromagnet with the push-pull rod, the whole device is more compact, the functional integration is improved, the switch of the magnetic field is realized by the power-on / power-off control of the electromagnet, the enrichment and cleaning operations are facilitated, the electromagnet can be located on the center line of the mixing chamber because the electromagnet is located at the connecting end of the push-pull rod body, the external magnetic field is uniformly distributed in the mixing chamber, the enrichment effect of the CTCs is improved, and the CTCs in some areas are not effectively enriched.

[0015] 2. The electromagnet is fixed inside the connecting end and wrapped by the wall of the connecting end, which means that the liquid in the mixing chamber cannot invade the electromagnet to cause the risk of electric leakage, and the electromagnet is hidden inside the connecting end, direct contact with the sample is avoided, and the risk of cross contamination is reduced.

[0016] 3. The smooth surface helps to adsorb the CTCs-magnetic bead compound, and the smooth surface reduces the possibility of the CTCs-magnetic bead compound adhering to the surface of the connecting end after the magnetic field is removed, so that the cleaning process is easier and more thorough.BRIEF DESCRIPTION OF DRAWINGS BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the schematic view of electromagnet in push-pull rod body of the utility model.

[0018] Figure 2 It is the internal structure schematic view of multifunctional push-pull rod of the utility model.

[0019] Figure 3 It is the structure schematic view of multifunctional push-pull rod of the utility model.

[0020] Figure 4 It is the structure schematic view of circulating tumor cell screening and separating device of the utility model.

[0021] In the figure, 100, push-pull rod body;110, connecting end;120, inner hole;130, wire hole;200, electromagnet;300, piston block;400, fixed support;500, driving element;600, shell;610, mixing cavity. DETAILED DESCRIPTION

[0022] The following is the specific embodiment of the utility model and combining with the drawings, the technical scheme of the utility model is further described, but the utility model is not limited to these embodiments.

[0023] As Figures 1 to 4 Indicated, a multifunctional push-pull rod, comprising: push-pull rod body 100 and electromagnet 200, one end of push-pull rod body 100 is set as connecting end 110, electromagnet 200 is installed in connecting end 110, electromagnet 200 is set as generating magnetic field when energized and removing magnetic field when de-energized.

[0024] Push-pull rod body 100 is used to push or pull piston block 300, so push-pull rod body 100 can be understood as piston rod, connecting end 110 is located at one end of push-pull rod body 100, connecting end 110 is used to connect piston block 300, and electromagnet 200 is installed in connecting end 110 simultaneously, and the design is used to ensure that electromagnet 200 is always in the position closest to piston block 300.Electromagnet 200 can generate magnetic field through electric current, and can also remove magnetic field by breaking electric current, and the size and power of electromagnet 200 need to be selected according to the required magnetic field intensity in actual application.

[0025] It needs to be supplemented here that electromagnet 200 can be arranged in connecting end 110, or on the outer surface of connecting end 110.When electromagnet 200 is arranged in connecting end 110, magnetic substance (CTCs-magnetic bead compound) is attracted by magnetic force on the outer surface of connecting end 110;When electromagnet 200 is arranged on the outer surface of connecting end 110, magnetic substance is directly attracted on the surface of electromagnet 200.

[0026] The working principle of the push-pull rod is that when the electromagnet 200 in the push-pull rod body 100 is powered on, it will generate a strong magnetic field, which can be used to attract the CTC-magnetic bead compound in the blood sample, thereby realizing effective enrichment of the CTCs. When the enrichment is completed, the power supply of the electromagnet 200 is cut off, the magnetic field disappears, and the attracted CTC-magnetic bead compound can be released from the vicinity of the electromagnet 200, and then the washing liquid is injected into the mixing cavity 610 for washing.

[0027] In the design, the push-pull rod and the electromagnet 200 are integrated together, reducing the complexity and volume of the device. The magnetic field can be applied or removed by power control, and the magnetic field strength can also be adjusted according to the need by adjusting the current size, adapting to the detection needs under different conditions.

[0028] Especially need to point out that, compared with directly inserting the magnet into the mixing cavity 610 in the glass tube or directly placing the magnet on the outer wall of the shell 600, the electromagnet 200 in the embodiment can be located on the center line of the mixing cavity 610, which means that the magnetic force distribution in the whole mixing cavity 610 is more uniform, which helps to ensure that the CTC-magnetic bead compound is uniformly attracted in the whole mixing cavity 610, avoiding the situation that some areas of CTCs are not effectively enriched.

[0029] As shown in Figures 1 to 4 On the basis of the above embodiment, the electromagnet 200 is fixedly installed in the inside of the connecting end 110, and when the electromagnet 200 is powered on, it can attract the magnetic material to the outer peripheral surface of the connecting end 110 through the wall part of the connecting end 110.

[0030] The electromagnet 200 is fixed in the inside of the connecting end 110, and the wall part of the connecting end 110 is wrapped outside the electromagnet 200, which means that the liquid in the mixing cavity 610 will not enter the electromagnet 200 to cause the risk of electric leakage, and the electromagnet 200 is hidden in the inside of the connecting end 110, avoiding direct contact with the sample and reducing the risk of cross contamination. The magnetic material is the CTC-magnetic bead compound, which will be enriched on the outer peripheral surface of the connecting end 110 due to the existence of the magnetic field, and then the piston block 300 can be moved by the push-pull rod body 100 to discharge the solution, at this time the liquid in the mixing cavity 610 is emptied, and the CTC-magnetic bead compound is retained in the mixing cavity 610 (the outer peripheral surface of the connecting end 110), which is convenient for subsequent washing.

[0031] As shown in Figures 1 to 3As shown, based on the above embodiment, the push-pull rod body 100 has an inner hole 120, one end of which extends into the connection end 110. The wall of the push-pull rod body 100 has a wire hole 130, which is connected to the other end of the inner hole 120. The electromagnet 200 is installed at one end of the inner hole 120 and located inside the connection end 110. The wire on the electromagnet 200 passes through the wire hole 130 and exits the inner hole 120.

[0032] The inner hole 120 is used to install the wires on the electromagnet 200 and the power supply magnet 200, and the wire through hole 130 allows the wires to pass through the inner hole 120 and connect to the power supply.

[0033] Based on the above embodiment, the outer peripheral surface of the connecting end 110 is made smooth. A smooth surface facilitates the adsorption of the CTCs-magnetic bead complex, and reduces the likelihood of the CTCs-magnetic bead complex adhering to the surface of the connecting end 110 after the magnetic field is removed, making the cleaning process easier and more thorough. In actual structures, the outer peripheral surface of the connecting end 110 can be polished (surface treated) or coated with a layer to further reduce the likelihood of CTCs-magnetic bead complex adhesion.

[0034] like Figures 1 to 3 As shown, based on the above embodiment, a piston block 300 is also included, and the connecting end 110 of the push-pull rod body 100 is fixedly connected to the center of the piston block 300. The push-pull rod body 100 can drive the piston block 300 to move, thereby controlling and achieving the effect of liquid transfer.

[0035] Based on the above embodiments, it also includes a fixed bracket 400 and a driving element 500. The driving element 500 is fixedly connected to the fixed bracket 400, and the push-pull rod body 100 is connected to the driving element 500. The driving element 500 is configured to drive the push-pull rod body 100 to move axially.

[0036] In the actual structure, the fixed bracket 400 is installed at the top of the device housing 600, and the drive element 500 is responsible for driving the axial movement of the push-pull rod body 100. The drive element 500 can be a motor, pneumatic cylinder, hydraulic cylinder, or other type of linear actuator. When the drive element 500 is activated, it drives the push-pull rod body 100 to move axially, thereby controlling the stroke position of the piston block 300 to achieve the effect of automated operation of the circulating tumor cell screening and separation device.

[0037] like Figures 1 to 4As shown, on the basis of the above embodiment, a circulating tumor cell screening and separating device includes a multifunctional push-pull rod, and further includes a shell 600, a mixing cavity 610 is arranged in the shell 600, the push-pull rod body 100 is connected with the shell 600 in an axially movable manner, the part of the push-pull rod body 100 containing the connecting end 110 extends into the mixing cavity 610, and the other part of the push-pull rod body 100 extends out of the mixing cavity 610 from the top of the shell 600.

[0038] The piston block 300 is located in the mixing cavity 610 and separates the mixing cavity 610 into an upper cavity and a lower cavity, and the electromagnet 200 is always located in the upper cavity. In specific operation, the driving element 500 moves the piston block 300 to the bottom of the mixing cavity 610 through the push-pull rod body 100, the blood sample and the SERS active nanomaterial are added into the upper cavity, after reaction and cultivation, the CTCs are combined with the magnetic beads to form CTC-magnetic bead complexes, then the electromagnet 200 is powered to generate a magnetic field, the CTC-magnetic bead complexes are attracted to the outer peripheral surface of the connecting end 110 through the magnetic field, then the driving element 500 drives the piston block 300 to move upwards through the push-pull rod body 100, the liquid in the upper cavity is discharged into the lower cavity, then the electromagnet 200 is powered off to remove the magnetic field, then the washing liquid is injected into the upper cavity, and the CTC-magnetic bead complexes are collected after multiple washing.

[0039] In the device, only the push-pull rod body 100 needs to be inserted into the top of the shell 600 and fixedly connected with the piston block 300 inside the mixing cavity 610, and no additional magnet is needed, so that the complexity and volume of the equipment are reduced; the electromagnet 200 is located at the center position, the generated magnetic field is uniformly distributed, the enrichment efficiency of the CTCs is improved, and the situation that the CTCs in some areas are not effectively enriched is avoided; the entire push-pull rod body 100 (piston structure) does not need to be manually operated, can be controlled through the driving element 500, the driving element 500 can be integrated with a control system to realize automatic operation, human intervention is reduced, and experimental efficiency and consistency are improved.

[0040] On the basis of the above embodiment, the connecting end 110 is always located in the mixing cavity 610 and the wire passing hole 130 is always located outside the mixing cavity 610 when the push-pull rod body 100 is at any stroke position.

[0041] It should be noted that all the directionality indications (such as up, down, left, right, front, back, etc.) in the embodiments of the utility model are only used to explain the relative positional relationship, movement condition and the like between components in a certain specific posture (as shown in the drawings), and if the specific posture changes, the directionality indications also change accordingly.

[0042] In addition, the descriptions such as "first", "second", "one" and the like in the present application are only for the purpose of description and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first" and "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "multiple" is at least two, for example, two, three, etc., unless otherwise specifically limited.

[0043] In the present application, unless otherwise specifically defined and limited, the terms "connection", "fixing" and the like should be broadly understood, for example, "fixing" can be fixed connection, or detachable connection, or integrated; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through intermediate medium, can be the internal communication of two elements or the interaction relationship of two elements, unless otherwise specifically limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0044] In addition, the technical solutions of each embodiment of the present application can be combined with each other, but it must be based on the realization of the ordinary skilled in the art, when the combination of technical solutions appears contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist, nor is it within the protection scope required by the present application.

Claims

1. A multi-functional push-pull rod characterized by, The utility model relates to a multifunctional push-pull rod, comprising: A push-pull rod body (100) and an electromagnet (200), one end of the push-pull rod body (100) is provided as a connecting end (110), the electromagnet (200) is installed on the connecting end (110), and the electromagnet (200) is arranged to generate a magnetic field when powered on and remove the magnetic field when powered off.

2. A multi-functional push-pull rod as claimed in claim 1, characterized in that: The electromagnet (200) is fixedly installed inside the connecting end (110), and the electromagnet (200) can adsorb a magnetic substance to the outer peripheral surface of the connecting end (110) through the wall part of the connecting end (110) when powered on.

3. A multi-functional push-pull rod as claimed in claim 2, wherein: The push-pull rod body (100) is provided with an inner hole (120), one end of the inner hole (120) extends to the inside of the connecting end (110), the wall part of the push-pull rod body (100) is provided with a wire passing hole (130), the wire passing hole (130) is in communication with the other end of the inner hole (120), the electromagnet (200) is installed at one end of the inner hole (120) and located inside the connecting end (110), and the wire on the electromagnet (200) passes out of the inner hole (120) through the wire passing hole (130).

4. A multi-functional push-pull rod as claimed in claim 2, wherein: The outer peripheral surface of the connecting end (110) is provided as a smooth surface.

5. A multi-functional push-pull rod as claimed in claim 2, wherein: Further comprising a piston block (300), the connecting end (110) of the push-pull rod body (100) is fixedly connected with the center of the piston block (300).

6. A multi-functional push-pull rod as claimed in claim 1, wherein: Further comprising a fixed support (400) and a driving element (500), the driving element (500) is fixedly connected with the fixed support (400), the push-pull rod body (100) is connected with the driving element (500), and the driving element (500) is arranged to be capable of driving the push-pull rod body (100) to move axially.

7. A circulating tumor cell screening and isolation device, comprising: The utility model relates to a multifunctional push-pull rod, comprising:

8. A circulating tumor cell screening and isolation device as claimed in claim 7, wherein: The connecting end (110) is always located inside the mixing cavity (610) and the wire passing hole (130) is always located outside the mixing cavity (610) when the push-pull rod body (100) is in any stroke position.

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