Household self-detection kit based on isothermal amplification

Abstract

A household self-detection kit based on isothermal amplification comprises a constant-temperature heating device and a detection magic cube, the constant-temperature heating device comprises a heating module arranged in a shell, a plurality of heating holes are evenly formed in the heating module, and the detection magic cube comprises a square frame plate on the upper portion and a plurality of detection pipe bodies evenly connected to the bottom face of the square frame plate. The detection pipe bodies and the heating holes are the same in number and matched in size, a sealing cover is connected to an upper port of the shell through a hinge, and the sealing cover and the shell are locked through a lock catch assembly; a collected sample can be heated through the heating module in the shell, meanwhile, the detection magic cube matched with the heating module is designed, a freeze-drying reagent is contained in the detection magic cube, the sample is dropwise added into the detection magic cube after being heated to be mixed with the freeze-drying reagent, heating amplification detection is conducted in the heating module, and operation and use are convenient and fast; the method can be used for self-inspection of a home environment and is beneficial to timely and fast obtaining of a detection result.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological sample extraction, detection and analysis, especially to a family self-checking kit based on constant temperature amplification. BACKGROUND

[0002] Molecular diagnosis, especially PCR technology, plays an important role in clinical detection. Pathogen infection detection technology based on nucleic acid amplification strategy is a conventional detection means. Emerging constant temperature amplification technologies, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) technology, can amplify at a relatively low temperature (37-65℃) and output results in 20 minutes. At the same time, isothermal amplification technology also eliminates the dependence on precise temperature control equipment. Constant temperature amplification technology is widely used in professional medical detection institutions and has been widely promoted in hospitals. However, there is a risk of secondary infection in pathogen infection detection in detection institutions and hospitals, and it is not conducive to obtaining detection results quickly and timely. Therefore, it is necessary to design a constant temperature amplification self-checking kit for home use. SUMMARY

[0003] To solve the above problems, the utility model provides a family self-checking kit based on constant temperature amplification.

[0004] The technical scheme of the utility model is: a family self-checking kit based on constant temperature amplification, which comprises a constant temperature heating device and a detection magic cube. The detection magic cube is vacuum packaged with an aluminum film bag. The constant temperature heating device comprises a heating module arranged in a shell. The shell is a square shell. A plurality of heating holes are uniformly arranged on the heating module. The lower part of the heating hole has a certain taper. The detection magic cube comprises a square frame plate at the upper part and a plurality of detection tube bodies uniformly connected to the bottom surface of the square frame plate. The number and size of the detection tube bodies match those of the heating holes. A sealing cover is connected to the upper end of the shell through a hinge. A rubber pad is arranged between the sealing cover and the shell. The sealing cover and the shell are locked through a lock assembly.

[0005] Preferably, an assembly gap is arranged between the heating module and the bottom plate of the shell. A fan is fixedly installed on the bottom plate in the assembly gap. A bottom air hole corresponding to the position of the fan is arranged on the bottom plate. Four butt joints are arranged on the bottom plate. Threaded blind holes are arranged in the butt joints. Screws connected to the butt joints are installed in the through holes at the four corners of the fan, thereby achieving the fixation of the fan.

[0006] Preferably, side air holes are arranged on the side plates around the shell. Concave parts are arranged at both ends and both sides of the heating module. An air flow channel is formed between the concave parts and the side plates of the shell to communicate the side air holes and the assembly gap.

[0007] Preferably, a 63°C indicator light and a 95°C indicator light are provided in the center of the front of the housing.

[0008] Preferably, the detection tubes on the detection cube are numbered sequentially on their sidewalls, and each detection tube contains a lyophilized reagent ball corresponding to its number. The lyophilized reagent ball is sealed with a silicone stopper at the vacuum line. The lyophilized reagent contains a visual colorimetric agent, which is orange when the test result is negative and green when the test result is positive.

[0009] Preferably, the lyophilized reagent spheres include novel coronavirus, influenza A virus, influenza B virus, respiratory adenovirus, human metapneumovirus, mycoplasma pneumoniae, respiratory syncytial virus, or parainfluenza virus types I-III.

[0010] Preferably, the upper port of the detection tube in the detection cube is provided with a silicone sealing plug.

[0011] The beneficial technical effects of this utility model are as follows: This utility model can heat the collected sample through the heating module inside the shell. At the same time, a detection cube matching the heating module is designed. The detection cube contains lyophilized reagent. After the sample is heated, it is dropped into the detection cube and mixed with the lyophilized reagent. The sample is then heated and amplified in the heating module for detection. The operation is convenient and quick. It can be used for self-testing in the home environment and facilitates timely and rapid test results. Attached Figure Description

[0012] Figure 1 This is one of the three-dimensional structural schematic diagrams of this utility model;

[0013] Figure 2 This is the second three-dimensional structural schematic diagram of this utility model;

[0014] Figure 3 This is a schematic diagram of the main structure of this utility model;

[0015] Figure 4 yes Figure 3 A schematic diagram of the AA-direction cross-section structure;

[0016] Figure 5 yes Figure 4 Schematic diagram of the BB-direction cross-section structure;

[0017] Figure 6 This is the third three-dimensional structural schematic diagram of this utility model.

[0018] In the diagram, 1. Outer shell, 11. Limiting platform, 12. Bottom vent, 13. Side vent, 14. Airflow channel, 15. Indicator light, 2. Sealing cover, 21. Hinge, 3. Heating module, 31. Heating hole, 41. Square frame plate, 42. Detection tube, 5. Fan, 6. Locking assembly, 7. Slide switch. DETAILED DESCRIPTION

[0019] Embodiment one, see attached Figures 1-3 A home self-test kit based on constant temperature amplification, comprising a constant temperature heating device and a detection magic cube, the constant temperature heating device comprises a heating module 3 arranged in the shell 1, a limiting seat 11 is arranged at the corner of the lower part of the shell to support the bottom surface of the heating module 3, a plurality of heating holes 31 are uniformly arranged on the heating module 3, the detection magic cube comprises a square frame plate 41 at the upper part and a plurality of detection tube bodies 42 connected to the bottom surface of the square frame plate, the number of detection tube bodies 42 is the same as that of the heating holes 31 and the sizes are matched, the heat inside the heating holes 31 can be efficiently transmitted into the detection tube bodies 42, a sealing cover 2 is connected to the upper end of the shell 1 through a hinge 21, the sealing cover 2 and the shell 1 are locked through a lock catch assembly 6, and the sealing cover 2 plays a heat preservation role, which can improve the heating efficiency of the heating module 3.

[0020] The middle part of the front of the shell 1 is provided with a 63℃ indicator lamp 15 and a 95℃ indicator lamp 15, and the heating temperature can be switched through a switch.

[0021] The side wall of the detection tube body 42 on the detection magic cube is sequentially marked with a number, and a freeze-dried reagent ball corresponding to the number is placed in the detection tube body 42, and different numbers correspond to different freeze-dried reagent balls, so that the sample can be placed in the detection tube body 42 corresponding to the freeze-dried reagent ball according to the detection item.

[0022] The freeze-dried reagent ball comprises a novel coronavirus, an influenza A virus, an influenza B virus, a respiratory adenovirus, a human metapneumovirus, a mycoplasma pneumoniae, a respiratory syncytial virus or a parainfluenza virus I-III, and can detect various pathogen infections, and the upper end of the detection tube body 42 in the detection magic cube is provided with a silica gel sealing plug.

[0023] The main components of the lysis preservation solution used in this embodiment include water, savonitis, EDTA, Tris-HCl, Tween 20, triton 100, etc., and the sealing oil used is paraffin oil.

[0024] ①Preparation: take out all the items in the self-test kit, power on the constant temperature heating device, turn the key to 95℃, the device starts to work, and the green light turns on when the temperature reaches 95℃;

[0025] ②Sampling: use a throat swab to sample, put the throat swab into the lysis preservation solution in the test tube, break the swab at the crease, tighten the test tube cap, and shake up and down to mix evenly;

[0026] ③Sample preparation: place the test tube containing the sample in the heating hole 31 of the heating module 3, cover the sealing cover 2 for heating, count 15 min, every 5 min during the period, take out the test tube and mix evenly, count after taking out the test tube, room temperature for 5 min, and turn the switch of the constant temperature heating device to 63℃;

[0027] ④Sample addition: open the outer package of the detection magic cube, remove the silica gel plug in the detection tube body 42, use a Pasteur pipette to suck the sample in the test tube and drop it into the detection tube body 42, add 3 drops of sample to each detection tube body 42;

[0028] ⑤Sealing: use a new Pasteur dropper to suck sealing oil, add 3 drops of sealing oil to each detection tube body 42, and cover the silica gel plug again;

[0029] ⑥Detection: when the 63℃ operation indicator light 15 green light is on, match the detection magic cube into the heating module 3, cover the sealing cover 2 tightly, and use the lock buckle assembly 6 to fix, count 30 min, take out after the end of the count, and observe the results;

[0030] ⑦Result interpretation: the freeze-dried reagent contains visual color developing agent, the detection result is orange when it is negative, and the detection result is green when it is positive;

[0031] ⑧Waste disposal: put all consumables into a garbage bag, spray 84 disinfectant, and discard after packaging.

[0032] Example two, see attached Figures 4-6 , this example is basically the same as example one, the same place is not described, the difference is: the assembly gap is provided between the heating module 3 and the bottom plate of the shell 1, the fan 5 is fixedly installed in the assembly gap, the bottom air hole 12 corresponding to the position of the fan 5 is arranged on the bottom plate, the fan 5 can send the external air into the bottom of the heating module 3 through the bottom air hole 12, and continuously exchange heat to cool the heating module 3; The side air holes 13 are arranged on the side plates of the shell 1, recesses are arranged at both ends and both sides of the heating module, the airflow channel 14 is formed between the recesses and the side plates of the shell 1, which connects the side air holes 13 and the assembly gap, the air flow after entering the shell for cooling the heating module 3 enters the airflow channel 14 upward, and is discharged from each side air hole 13 to the shell, which protects the internal components of the heating module 3 and improves its service life.

Claims

1. A home self-test kit based on isothermal amplification, characterized by: The constant temperature heating device comprises a heating module arranged in a shell, and a plurality of heating holes are uniformly arranged on the heating module; the detection Rubik's Cube comprises a square frame plate at the upper portion and a plurality of detection tube bodies uniformly connected to the bottom surface of the square frame plate, the number of the detection tube bodies is the same as that of the heating holes and the sizes are matched, a sealing cover is hingedly connected to the upper end of the shell, and the sealing cover and the shell are locked by a lock buckle assembly.

2. The home test kit based on isothermal amplification according to claim 1, characterized in that: The heating module and the bottom plate of the shell are provided with an assembly gap, a fan is fixedly installed on the bottom plate in the assembly gap, and a bottom air hole corresponding to the position of the fan is arranged on the bottom plate.

3. The home test kit based on isothermal amplification according to claim 2, characterized in that: The side plates around the shell are each provided with a side air hole, the heating module is provided with recessed portions at the two ends and the two sides, and the recessed portions and the side plates of the shell form an airflow channel connecting the side air holes and the assembly gap.

4. The home test kit based on isothermal amplification according to claim 1, characterized in that: The middle portion of the front face of the shell is provided with a 63 DEG C indicator lamp and a 95 DEG C indicator lamp.

5. The home test kit based on isothermal amplification according to claim 1, characterized in that: The side walls of the detection tube bodies on the detection Rubik's Cube are sequentially marked with numbers, and freeze-dried reagent balls corresponding to the numbers are placed in the detection tube bodies.

6. The home test kit based on isothermal amplification according to claim 5, characterized in that: The freeze-dried reagent balls comprise a novel coronavirus, an influenza A virus, an influenza B virus, a respiratory adenovirus, a human metapneumovirus, a mycoplasma pneumoniae, a respiratory syncytial virus or a parainfluenza virus I-III.

7. The home test kit based on isothermal amplification according to claim 1, characterized in that: The upper end of the detection tube body in the detection Rubik's Cube is provided with a silica gel sealing plug.

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