Kit for detecting sunset yellow

Abstract

The utility model relates to the technical field of synthetic pigment detection kits, in particular to a kit for detecting sunset yellow. The kit comprises a kit body, a bearing assembly and a detection assembly, the detection assembly comprises an elisa plate coated with sunset yellow hapten, a plurality of suction pipes and a plurality of solvent bottles; the kit body comprises an upper-layer space and a lower-layer space which are separated from each other; the elisa plate and the suction tube are placed in the upper-layer space; a plurality of solvent bottles are placed in the lower layer space; the bearing assembly comprises a bearing plate; the bearing plate is arranged in the lower layer space and is detachably connected with the bottom of the kit body; and the solvent bottle is detachably connected with the bearing plate. The structural design of the kit is ingenious, partition arrangement is adopted, when the kit is used, the bearing plate can be directly taken out, different solvent bottles do not need to be found, operation can be directly carried out on the bearing plate, and the solvent bottles can be taken down from the bearing plate when necessary. The whole detection operation process is simplified and convenient, and the condition that solvents are mixed with food or bottles fall down is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to synthetic pigment detection kit technical field, specifically, relate to a reagent box for detecting sunset yellow. BACKGROUND

[0002] More than 50 synthetic pigments (food colorants) are allowed to be used in China, and more than 3000 non-food pigments are known. The over-standard use of synthetic pigments, or long-term consumption by consumers, can cause great harm to the human body.

[0003] As one of the longest used synthetic pigments, sunset yellow is an artificial synthetic colorant that can increase the appearance of color. Sunset yellow is also known as sunset yellow, sunset yellow, orange, and food yellow No. 3. Its molecular formula is C 16 H 10 N2Na2O7S2, and its relative molecular mass is 452.38, which is an azo pigment. Sunset yellow is an orange-red powder or granules, odorless. Easily soluble in water, propylene glycol, slightly soluble in ethanol, the solubility at 25 DEG C is: 19.0% (water), 3.0% (50% ethanol), 20% (50% glycerol), 7.0% (50% propylene glycol). Insoluble in oil, neutral and water solution is orange yellow, red brown with alkali. Strong hygroscopicity, heat resistance and light resistance, fading when reduced. Orange liquid in concentrated sulfuric acid. Easy to color, high fastness.

[0004] The detection of sunset yellow is mostly by high performance liquid chromatography, and there are also thin layer chromatography, spectrophotometer method, etc. in the prior art, however, the biggest disadvantage of the above method is complex operation and inconvenience to carry. At present, ELISA detection method is used to simplify the detection process and improve the portability by using reagent box to preliminarily detect sunset yellow.

[0005] However, the reagent box on the market is to place the materials used for detection in an empty box, which still has problems such as complicated operation, easy to pour solution bottle and easy to confuse solvent when used. UTILITY MODEL CONTENT

[0006] The utility model aims to provide a reagent box for detecting sunset yellow, which has a clever structure design, solid-liquid materials are placed separately, and materials are easy to take when used; the support plate can be taken out directly when used, different solvent bottles do not need to be found, and operation can be directly carried out on the support plate, and the solvent bottle can be taken off from the support plate when necessary. The operation process of the whole detection is simplified and convenient, and solvent mixing or bottle pouring can be avoided.

[0007] In order to achieve the above purpose, the preferred solution adopted by the utility model is:

[0008] The kit for detecting sunset yellow comprises a kit body, a supporting assembly and a detection assembly; the detection assembly comprises an enzyme-labeled plate coated with sunset yellow hapten, a plurality of suction tubes and a plurality of solvent bottles; the solvent bottles comprise empty bottles for loading sample preparation liquid to be loaded and detected, and carrier bottles loaded with sunset yellow standard, labeled enzyme, sunset yellow specific antibody, substrate developing liquid or reaction termination liquid; the kit body comprises an upper space and a lower space separated from each other; the enzyme-labeled plate and the suction tubes are placed in the upper space; the plurality of solvent bottles are placed in the lower space; the supporting assembly comprises a supporting plate; the supporting plate is arranged in the lower space and detachably connected with the bottom of the kit body; and the solvent bottles are detachably connected with the supporting plate.

[0009] Further, in the preferable embodiment of the utility model, the supporting assembly further comprises a plurality of limiting assemblies; one side of the supporting plate away from the bottom is concavely provided with a plurality of limiting grooves for placing the solvent bottles one by one; the limiting assemblies are arranged in the limiting grooves; the limiting assembly comprises two sets of limiting pieces arranged symmetrically; the limiting piece comprises an elastic piece and an abutting piece connected with each other; one end of the elastic piece away from the abutting piece is connected with the inner wall of the limiting groove; when the solvent bottle is placed in the limiting groove, the elastic piece is extruded, and the abutting piece abuts against the solvent bottle.

[0010] Further, in the preferable embodiment of the utility model, the abutting piece has an abutting surface abutting against the solvent bottle; and the abutting surface is an arc surface.

[0011] Further, in the preferable embodiment of the utility model, the supporting plate is provided with a solvent identification label corresponding to the limiting groove.

[0012] Further, in the preferable embodiment of the utility model, the supporting plate and the bottom are connected through magic tape.

[0013] The kit for detecting sunset yellow has the following beneficial effects:

[0014] The kit for detecting sunset yellow comprises a kit body, a supporting assembly and a detection assembly; according to the structural design of the kit body, the supporting assembly and the detection assembly and the connection mode design therebetween, the obtained kit can realize the following effects:

[0015] (1) The solid-liquid materials are placed separately in different areas, so that the materials are convenient to take during use, and cross contamination caused by solvent bottle breakage is avoided;

[0016] (2) the support plate is detachably connected with the kit body, when not in use, the support plate is fixed at the bottom of the kit body, and the solvent bottle is fixed on the support plate, so that mutual collision and breakage and the like are avoided; when in use, the support plate is directly taken out from the kit body, different solvent bottles do not need to be found, and sample solution can be directly configured on the support plate or different solvents are directly taken, and the solvent bottle can also be taken off from the support plate. During the whole detection process, solvent mixing or bottle overturning and the like can be avoided, the detection operation time is saved, and the detection process is simplified.

[0017] (3) simple and portable: most citizens do not understand the harm of sunset yellow, and cannot detect whether the milk tea and the like contain sunset yellow, the sunset yellow kit provided by the application is simple, small, portable and easy to operate, so that the sunset yellow in the milk tea and the like can be quickly detected, and the application is beneficial to popularization to the society.

[0018] (4) the kit provided by the application is based on immunology, and is quickly and conveniently detected through specific immune response, which is beneficial to guide the future food safety rapid detection trend and create a pioneer field for future food safety detection problems.

[0019] (5) the kit provided by the application has a wide range of applicable detection samples and low detection cost. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced as follows, and it should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope, and for those skilled in the art, other related drawings can also be obtained without creative labor on the premise of the drawings.

[0021] Fig. 1 the cross-sectional view of the kit for detecting sunset yellow provided by the embodiments of the present application without placing a solvent bottle;

[0022] Fig. 2 the cross-sectional view of the kit for detecting sunset yellow provided by the embodiments of the present application with a solvent bottle;

[0023] Fig. 3 the top view structure schematic view of the support assembly and the solvent bottle connection provided by the embodiments of the present application;

[0024] Icon: 10 - a kit for detecting sunset yellow, 100 - a kit body, 200 - a supporting assembly, 300 - an enzyme-labeled plate, 400 - a suction tube, 500 - a solvent bottle, 600 - a magic tape, 110 - an upper space, 120 - a lower space, 210 - a supporting plate, 220 - a limiting piece, 211 - a limiting groove, 221 - an elastic piece, 222 - an abutting piece, 212 - an identification tag. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. The components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0027] It should be noted that: similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0028] Embodiment 1

[0029] The following will be described in conjunction with the accompanying drawings Figs. 1-3 Further description of the present application:

[0030] The present application provides a kit 10 for detecting sunset yellow, please refer to Figs. 1-3 The kit 10 for detecting sunset yellow includes a kit body 100, a supporting assembly 200 and a detection assembly (not marked in the figure).

[0031] Among them, the detection assembly includes an enzyme-labeled plate 300 coated with sunset yellow hapten, a plurality of suction tubes 400, a plurality of solvent bottles 500; the solvent bottle 500 includes an empty bottle for configuring liquid of the sample to be detected, and a carrier bottle loaded with sunset yellow standard, labeled enzyme, sunset yellow specific antibody, substrate developing liquid or reaction termination liquid.

[0032] In the embodiment, the kit body 100 comprises a separated upper space 110 and a lower space 120. The enzyme-labeled plate 300 and the pipette 400 are placed in the upper space 110, and the plurality of solvent bottles 500 are placed in the lower space 120.

[0033] In the embodiment, the support assembly 200 comprises a support plate 210 and a plurality of limiting assemblies (not labeled in the figure). The support plate 210 is arranged in the lower space 120 and detachably connected with the bottom of the kit body 100. In the embodiment, the support plate 210 and the bottom of the kit body 100 are connected by the magic tape 600.

[0034] In the embodiment, the solvent bottles 500 are detachably connected with the support plate 210. Specifically, the side of the support plate 210 away from the bottom is concave with a plurality of limiting grooves 211 for placing the solvent bottles 500 one by one, and the limiting assemblies are arranged in the limiting grooves 211. In the embodiment, specifically, the limiting assemblies comprise two groups of limiting members 220 arranged symmetrically. The limiting member 220 comprises an elastic member 221 and an abutting member 222 connected with each other, and the end of the elastic member 221 away from the abutting member 222 is connected with the inner wall of the limiting groove 211. In the embodiment, the abutting member 222 has an abutting surface abutting with the solvent bottle 500, and the abutting surface is an arc surface, which is beneficial for the solvent bottle 500 to be smoothly placed into the limiting groove 211. When the solvent bottle 500 is placed in the limiting groove 211, the elastic member 221 is extruded, and the abutting member 222 abuts with the solvent bottle 500. At this time, the solvent bottle 500 is fixed on the support plate 210.

[0035] In the embodiment, the solvent identification labels 212 are attached on the support plate 210 corresponding to the positions of the limiting grooves 211.

[0036] The kit 10 for detecting sunset yellow provided in the embodiment is used as follows: the kit body 100 is opened, the support plate 210 is taken out from the bottom of the kit body 100, the enzyme-labeled plate 300 and the pipette 400 are taken out, and the sample solution is configured in the empty solvent bottle 500 by using the pipette 400 to obtain the sample to be tested. Subsequently, the sunset yellow standard or the sample to be tested is added into the hole of the enzyme-labeled plate 300 by using the pipette 400, then the enzyme-labeled sunset yellow specific antibody is added into each hole by using the pipette 400, and then incubation and washing are performed, then the substrate color developing solution is added by using the pipette 400 for color development, then the reaction termination solution is added by using the pipette 400, and finally the sunset yellow content in the sample to be tested is inferred by comparing the colors of the sunset yellow standard and the sample to be tested.

[0037] In summary, the kit 10 for detecting sunset yellow provided by the embodiment has a clever structure design, the solid-liquid materials are placed separately, the materials are convenient to take when used, the supporting plate 210 can be directly taken out when used, different solvent bottles 500 are not needed to be found, the operation can be directly performed on the supporting plate 210, and the solvent bottles 500 can be taken off from the supporting plate 210 when necessary. The whole operation process of detection is simplified and convenient, and the mixing of solvents or the situation of pouring bottles is avoided.

[0038] The preferred embodiments of the utility model are merely used for limiting the utility model, and the utility model can be changed and varied for the person skilled in the art. Any modification, equivalent replacement, improvement and the like within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A kit for detecting sunset yellow, characterized in that, The kit comprises a kit body, a supporting assembly and a detection assembly; The detection assembly comprises an enzyme-labeled plate coated with sunset yellow hapten, a plurality of pipettes and a plurality of solvent bottles; the solvent bottles comprise empty bottles for sample preparation solution and bottles loaded with sunset yellow standard, labeled enzyme, sunset yellow specific antibody, substrate developing solution or reaction termination solution; The kit body comprises a separated upper space and a lower space; the enzyme-labeled plate and the pipettes are placed in the upper space; The plurality of solvent bottles are placed in the lower space; The supporting assembly comprises a supporting plate; the supporting plate is arranged in the lower space and detachably connected with the bottom of the kit body; the solvent bottles are detachably connected with the supporting plate.

2. The kit for detecting sunset yellow according to claim 1, characterized by, The supporting assembly further comprises a plurality of limiting assemblies; The side of the supporting plate away from the bottom is concavely provided with a plurality of limiting grooves for placing the solvent bottles one by one; the limiting assemblies are arranged in the limiting grooves; The limiting assembly comprises two sets of limiting members arranged in symmetry; the limiting member comprises an elastic member and an abutting member connected with each other; the end of the elastic member away from the abutting member is connected with the inner wall of the limiting groove; when the solvent bottle is placed in the limiting groove, the elastic member is extruded, and the abutting member abuts against the solvent bottle.

3. The kit for detecting sunset yellow according to claim 2, characterized in that, The abutting member has an abutting surface abutting against the solvent bottle; the abutting surface is an arc surface.

4. The kit for detecting sunset yellow according to claim 2, characterized by, The supporting plate is provided with solvent identification labels corresponding to the limiting grooves.

5. The kit for detecting sunset yellow according to claim 1, characterized by, The supporting plate and the bottom are connected by magic tape.

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