Syphilis non-specific antibody detection device

Abstract

The utility model provides a syphilis non-specific antibody detection device, and belongs to the technical field of medical instruments. The problem of misreading of a critical sample due to manual operation and reading in the existing syphilis non-specific antibody detection is solved. The syphilis non-specific antibody detection device comprises a transparent sample cup, an optical detection system and a stirring structure used for uniformly mixing a sample and a reagent, the optical detection system comprises a transmitting end arranged on one side of the sample cup and a receiving end arranged on the other side of the sample cup, and the transmitting end is used for transmitting light to the receiving end; the receiving end is used for receiving and processing the light penetrating through the sample cup. A detection process curve is drawn through an optical detection system, the reaction value of the whole stage can be tested, and a positive detection result value can be accurately given.

Description

TECHNICAL FIELD

[0001] The utility model belongs to medical instrument technical field relates to a syphilis non specific antibody's detection device. BACKGROUND

[0002] At present, the detection of syphilis non specific antibody basically adopts two test methods of TRUST and RPR, the sample is handled on the test paper card by the operator, and then the sample is placed on the horizontal oscillator to run the test for 8 minutes, and the test personnel distinguish and judge the result to be negative or positive. Since manual operation and reading are adopted, critical samples may be misread, there is no detailed quantitative result, the result is only negative and positive, the positive degree needs to be further diluted and tested, and the detection result is also the dilution ratio, without exact detection value, the result cannot reflect the situation of the detection process. UTILITY MODEL CONTENT

[0003] The utility model discloses a syphilis non specific antibody's detection device that can automatically identify and read the detection result.

[0004] The utility model discloses a syphilis non specific antibody's detection device that can automatically identify and read the detection result.

[0005] The syphilis non specific antibody's detection device includes transparent sample cup, optical detection system and the stirring structure for mixing sample and reagent, the optical detection system includes the emission end of being located at one side of sample cup and the receiving end of being located at the other side of sample cup, the emission end is used for emitting light to the receiving end, and the receiving end is used for receiving and processing the light through the sample cup.

[0006] The emission end and the receiving end are oppositely arranged, the sample cup is located between the emission end and the receiving end, and the stirring structure is used for mixing the sample and the reagent in the sample cup. The reagent for detection is added during the test, and the detection curve will change greatly due to the influence of the color of the reagent.

[0007] In the syphilis non specific antibody's detection device, the receiving end includes a photoelectric sensor, a filter circuit, an operational amplifier and an AD sampling chip, the signal output end of the photoelectric sensor is connected with the input end of the filter circuit, the output end of the filter circuit is connected with the input end of the operational amplifier, the output end of the operational amplifier is connected with the analog input end of the AD sampling chip, and the AD sampling chip converts the electric signal into a digital signal.

[0008] Photoelectric sensors convert light into electrical signals. Filtering circuits remove unwanted frequency components, retaining only the useful electrical signals. Operational amplifiers (op-amps) amplify these signals for subsequent processing. High-precision AD sampling chips convert the electrical signals into digital signals and send them to microcontrollers, computers, or other digital processing devices for further processing, such as data analysis, storage, or display.

[0009] In the aforementioned detection device for non-specific antibodies against syphilis, the emitting end is driven by a constant current source. The high-precision constant current source ensures that the light emitted from the emitting end is constant.

[0010] In the above-mentioned detection device for non-specific antibodies against syphilis, the stirring structure includes a motor located below the sample cup and a magnetic stir bar located inside the sample cup. A magnet is mounted on the rotating shaft of the motor, and when the rotating shaft rotates, the magnet can drive the magnetic stir bar to rotate.

[0011] The detection method for non-specific antibodies against syphilis includes the following steps:

[0012] Step 1: Place the sample and the magnetic stir bar together into the sample cup, and place the sample cup between the transmitter and receiver;

[0013] Step 2: Start the motor and optical detection system;

[0014] Step 3: Add the detection reagent to the sample cup;

[0015] Step 4: The sample and reagents are thoroughly mixed using a magnetic stir bar;

[0016] Step 5: The photoelectric sensor converts the received light into an electrical signal, the AD sampling chip converts the electrical signal into a digital signal, and the computer plots the detection process curve.

[0017] When the sample is negative, the sample and reagent do not react, the light does not change, and the test result is approximately a straight line; when the sample is positive, the sample and reagent undergo an agglutination reaction, and the test process curve will change significantly.

[0018] In the above-mentioned detection method for non-specific antibodies against syphilis, the detection process curve is a detection value-time curve, with the horizontal axis representing the detection time and the vertical axis representing the detection value.

[0019] The detection process curve is divided into three stages: stage 1 is the detection value when the sample is first added, stage 2 is the detection value when the reagent is added, and stage 3 is the reaction process after the reagent is added.

[0020] After the reaction is complete, the value at each time point can be obtained by detecting the process curve, thus obtaining the quantitative detection value, maximum value, and other detection results of the sample.

[0021] In the above-mentioned method for detecting non-specific antibodies against syphilis, the reagent is toluidine red dye (TRUST) or colloidal particles (RPR) coated with BCG protein and phospholipids.

[0022] Compared with the prior art, the present invention has the following advantages:

[0023] The detection device has a reasonable structural design, which can fully mix the sample and reagents, and plot the detection process curve through the optical detection system. It can test the reaction value of the entire stage and give an accurate positive detection result value. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the detection device.

[0025] Figure 2 This is a connection diagram of the receiving end.

[0026] Figure 3 It is a graph showing the detection process.

[0027] In the diagram, 1 is the sample cup; 2 is the transmitter; 3 is the receiver; 31 is the photoelectric sensor; 32 is the filter circuit; 33 is the operational amplifier; 34 is the AD sampling chip; 4 is the motor; 5 is the magnetic stir bar; and 6 is the magnet. Detailed Implementation

[0028] The following are specific embodiments of the present invention, which are described in conjunction with the accompanying drawings. However, the present invention is not limited to these embodiments.

[0029] like Figure 1 The illustrated device for detecting non-specific antibodies against syphilis includes a transparent sample cup 1, an optical detection system, and a stirring structure for mixing the sample and reagents within the sample cup 1. The optical detection system includes an emitter 2 located on one side of the sample cup 1 and a receiver 3 located on the other side of the sample cup 1, with the emitter 2 and receiver 3 positioned opposite each other. The sample cup 1 is located between the emitter 2 and receiver 3. The emitter 2 emits light to the receiver 3, and the receiver 3 receives and processes the light transmitted through the sample cup 1. During the test, reagents are added, and the detection curve will vary significantly due to the color of the reagents.

[0030] like Figure 2 As shown, the receiver 3 includes a photoelectric sensor 31, a filter circuit 32, an operational amplifier 33, and an AD sampling chip 34. The signal output terminal of the photoelectric sensor 31 is connected to the input terminal of the filter circuit 32, the output terminal of the filter circuit 32 is connected to the input terminal of the operational amplifier 33, and the output terminal of the operational amplifier 33 is connected to the analog input terminal of the AD sampling chip 34. The AD sampling chip 34 converts the electrical signal into a digital signal.

[0031] The photoelectric sensor 31 converts light into an electrical signal, the filter circuit 32 removes unwanted frequency components and retains only the useful electrical signal, the operational amplifier 33 amplifies the electrical signal for subsequent processing, and the high-precision AD sampling chip 34 converts the electrical signal into a digital signal and sends it to a microcontroller, computer or other digital processing device for further processing, such as data analysis, storage or display.

[0032] The transmitter 2 is driven by a high-precision constant current source, ensuring that the light emitted by the transmitter 2 is constant. Specifically, the transmitter 2 is a laser emitter or an LED light.

[0033] like Figure 1 As shown, the stirring structure includes a motor 4 located below the sample cup 1 and a magnetic stir bar 5 located inside the sample cup 1. The rotating shaft of the motor 4 is vertically upward and coaxial with the sample cup 1. A magnet 6 is mounted on the upper end of the rotating shaft of the motor 4. When the rotating shaft of the motor 4 rotates, it drives the magnet 6 to rotate. When the magnet 6 rotates, it drives the magnetic stir bar 5 located inside the sample cup 1 to rotate through magnetic force, thereby mixing the sample and reagent.

[0034] The detection method for non-specific antibodies against syphilis includes the following steps:

[0035] Step 1: Place the sample and magnetic stir bar 5 together into sample cup 1, and place sample cup 1 between transmitter 2 and receiver 3;

[0036] Step 2: Start motor 4 and the optical detection system;

[0037] Step 3: Add the detection reagent to sample cup 1;

[0038] Step 4: The sample and reagents are thoroughly mixed under the action of the magnetic stir bar 5;

[0039] Step 5: The photoelectric sensor 31 converts the received light into an electrical signal, the AD sampling chip 34 converts the electrical signal into a digital signal, and the computer plots the detection process curve.

[0040] The detection process curve is a test value-time curve, with the horizontal axis representing the detection time and the vertical axis representing the test value. When the sample is negative, the sample and reagent do not react, the light does not change, and the test result is approximately a straight line. When the sample is positive, the sample and reagent undergo an agglutination reaction, and the detection process curve will show a significant change.

[0041] The detection process curve consists of three stages: Stage 1 shows the detection value immediately after sample addition, Stage 2 shows the detection value upon reagent addition, and Stage 3 shows the reaction process after reagent addition. After the reaction is complete, the detection process curve provides the value at each time point, thus yielding the quantitative detection value, maximum value, and other detection results for the sample.

[0042] In this embodiment, the reagent is toluidine red dye (TRUST) or colloidal particles (RPR) coated with BCG protein and phospholipids.

[0043] The specific embodiments described herein are merely illustrative examples illustrating the spirit of this utility model. Those skilled in the art to which this utility model pertains may make various modifications or additions to the described specific embodiments or use similar methods to replace them, without departing from the spirit of this utility model or exceeding the scope defined by the appended claims.

Claims

1. A device for detecting non-specific antibodies to syphilis, characterized in that, The application relates to a transparent sample cup (1), an optical detection system and a stirring structure for mixing samples and reagents, the optical detection system comprising a transmitting end (2) arranged on one side of the sample cup (1) and a receiving end (3) arranged on the other side of the sample cup (1), the transmitting end (2) being used for transmitting light to the receiving end (3), and the receiving end (3) being used for receiving and processing the light transmitted through the sample cup (1).

2. The device for detecting non-specific antibodies to syphilis according to claim 1, characterized in that, The receiving end (3) comprises a photoelectric sensor (31), a filter circuit (32), an operational amplifier (33) and an AD sampling chip (34), the signal output end of the photoelectric sensor (31) is connected with the input end of the filter circuit (32), the output end of the filter circuit (32) is connected with the input end of the operational amplifier (33), the output end of the operational amplifier (33) is connected with the analog input end of the AD sampling chip (34), and the AD sampling chip (34) converts an electric signal into a digital signal.

3. The device for detecting non-specific antibodies to syphilis according to claim 1, characterized in that, The transmitting end (2) is driven by a constant current source.

4. The device for detecting non-specific antibodies to syphilis according to claim 1, characterized in that, The stirring structure comprises a motor (4) arranged below the sample cup (1) and a magnetic stirring rod (5) arranged in the sample cup (1), a magnet (6) is arranged on the rotating shaft of the motor (4), and the magnet (6) can drive the magnetic stirring rod (5) to rotate when the rotating shaft rotates.

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