Drainage device for protein purification equipment

Abstract

The utility model relates to the technical field of biological protein processing and production, in particular to a drainage device for protein purification equipment, which comprises a connecting base, a connecting seat, a connecting sleeve, a connecting vertical pipe, a supporting seat, a dense separation net, a molecular sieve filling part, a top net cover, a sampling pipe and a drainage pipe, an opening is formed in the middle of the upper end of the connecting base, the connecting sleeve is fixedly connected to the surface of the edge position of the opening of the connecting base, internal threads are formed in the inner surface of the connecting sleeve, external threads are arranged at the lower end of the connecting vertical pipe, and the lower end of the connecting vertical pipe is in threaded connection with the inner surface of the connecting sleeve. The supporting seat is fixedly connected to the upper surface of the middle position of the connecting base, the dense separation net is connected to the upper end of the supporting seat in a matched mode, and the device has the effects of being high in operation convenience degree, high in filtering efficiency, capable of improving the drainage speed, convenient to clean and not prone to waste.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological protein processing production, and in particular to a protein purification equipment drainage device. BACKGROUND

[0002] In the production process of biological products, it is necessary to separate proteins. In the existing enzymatic technology, a large amount of water is mixed in the obtained protein solution, and the content of the protein is low. In order to perform the next operation, the water in the protein solution needs to be removed, and at this time the form of the protein, cell and other materials also needs to be processed, and the form cannot be damaged, so the centrifugal equipment cannot be used for water removal operation. In the existing operation method, liquid molecular sieve is used for water filtration. In use, the liquid molecular sieve is first placed at the bottom of the test tube, and then the protein or cell product solution is added. Since the diameter of the molecular sieve is small, the cells, proteins and other macromolecules are blocked in the upper liquid, and the water passes through the middle of the molecular sieve, completing the concentration of the upper protein or cell solution. However, in the existing filtration process, glass tubes, beakers and other devices are used as raw materials, which are assembled into temporary filtration devices by opening holes at the bottom after heating. Although this device can achieve filtration, the assembly, liquid taking and cleaning operations are complicated, the efficiency is low, the stability is not good, and the liquid is easy to spill, resulting in waste. Therefore, it is necessary to improve the structure of the existing protein drainage equipment to solve these problems. SUMMARY

[0003] In view of the deficiencies in the prior art, the purpose of the present application is to provide a protein purification equipment drainage device which is high in operation convenience, high in filtration efficiency, high in drainage speed, convenient to clean and not easy to cause waste.

[0004] The above application purpose of the present application is realized by the following technical scheme:

[0005] A kind of protein purification equipment drain device, including: connecting base, connecting seat, connecting sleeve, connecting vertical pipe, support seat, tight screen, molecular sieve filling part, top mesh cover, sampling tube and drain pipe, the upper end of the connecting base is matched with the connecting seat, the upper end of the connecting seat is provided with open, the surface of the connecting sleeve is fixedly connected at the edge position of the open of connecting seat, the inner surface of connecting sleeve is provided with internal thread, the lower end of the connecting vertical pipe is provided with external thread, the lower end of the connecting vertical pipe is threadedly connected with the inner surface of connecting sleeve, the upper surface of the connecting base is fixedly connected at middle position, the upper end of the support seat is matched with the connecting seat, the lower end of the top mesh cover is in contact with the upper surface of the open of connecting seat, the molecular sieve filling part is located between the top mesh cover and the tight screen, the sampling tube is arranged at the upper end of the top mesh cover corresponding to the side of the connecting base, the drain pipe is arranged at the lower end of the connecting base near the edge position.

[0006] Optionally, it further includes a control valve, which is arranged on the sampling tube.

[0007] Optionally, it further includes a mounting seat, which is fixedly connected in annular array on the lower surface of the connecting base.

[0008] Optionally, it further includes a handle, which is fixedly connected on the two side surfaces of the upper end of the connecting seat.

[0009] Optionally, it further includes a flow slowing plate, which is arranged on the inner wall of the connecting vertical pipe near the upper end, and there is a gap between the edge of the flow slowing plate and the inner wall of the connecting vertical pipe.

[0010] Optionally, it further includes a scale line, which is arranged equidistantly on the outer surface of the connecting vertical pipe.

[0011] Optionally, it further includes a mounting hole, which is respectively provided through on the mounting seat.

[0012] Optionally, it further includes a sealing washer, which is arranged in the gap between the lower end of the connecting vertical pipe and the upper end of the connecting seat.

[0013] The protein purification equipment drain device can limit the filling position of the molecular sieve through the combined structure, and the concentrated protein solution after drainage can be easily taken out, the operation process is relatively simple, the device can be disassembled and cleaned, and the efficiency in the operation process is effectively improved.

[0014] The connecting base at the lower end has a larger diameter than the connecting vertical pipe at the upper end, so the area for filtration at the lower end is large, the water can pass through a large area, and the drainage speed is improved, and the concentration speed of the protein liquid above is accelerated.

[0015] The height of the connecting vertical pipe of the protein purification equipment drainage device can be set according to actual needs, and when the height is higher, the pressure on the lower molecular sieve filling part is greater, thereby helping to accelerate the drainage of water. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is a schematic diagram of the overall partial cut structure provided by the embodiment of the present application;

[0017] Figure 2 is a schematic diagram of the partial assembly partial cut structure provided by the embodiment of the present application;

[0018] Figure 3 is a schematic diagram of the connecting seat cut structure provided by the embodiment of the present application;

[0019] Figure 4 is a schematic diagram of the bottom structure provided by the embodiment of the present application;

[0020] Figure 5 is a schematic diagram of the enlarged structure at A provided by the embodiment of the present application.

[0021] Reference signs: 1, connecting base; 2, connecting seat; 3, connecting sleeve; 4, connecting vertical pipe; 5, support seat; 6, close net; 7, molecular sieve filling part; 8, top net cover; 9, sampling tube; 10, drain pipe; 11, control valve; 12, mounting seat; 13, handle; 14, slow flow plate; 15, scale line; 16, mounting hole; 17, sealing washer. DETAILED DESCRIPTION

[0022] The present application will be further described in detail below with reference to the accompanying drawings.

[0023] In order to more clearly understand the technical solutions exhibited in the embodiments of the present application, first, the working principle of the existing protein purification equipment drainage device is introduced.

[0024] In the process of draining the existing protein and cell products, there is a lack of corresponding matching devices. In order to improve the content of protein in the solution and reduce the cost of subsequent operation, a biological molecular sieve is often used to separate water. Tubes, beakers and other structures are used on the equipment. In the process of filtration, the operation is not convenient. In addition to slow filtering speed, there are problems such as easy spilling and inconvenient cleaning. Therefore, the existing drainage and concentration operation equipment needs to be designed to improve the speed and convenience in the use process, and thus the design scheme of the present application is proposed.

[0025] Please refer to Figures 1 to 3The protein purification equipment drainage device disclosed by the embodiment of the application comprises a connecting base 1, a connecting seat 2, a connecting sleeve 3, a connecting vertical pipe 4, a supporting seat 5, a sealed net 6, a molecular sieve filling part 7, a top mesh cover 8, a sampling pipe 9 and a drainage pipe 10. The connecting seat 2 is matched and connected to the upper end of the connecting base 1. An open end is arranged at the middle part of the upper end of the connecting seat 2. The connecting sleeve 3 is fixedly connected to the surface of the open end edge position of the connecting seat 2. The inner surface of the connecting sleeve 3 is provided with an internal thread. The lower end of the connecting vertical pipe 4 is provided with an external thread. The lower end of the connecting vertical pipe 4 is threadedly connected to the inner surface of the connecting sleeve 3. The supporting seat 5 is fixedly connected to the upper surface of the middle position of the connecting base 1. The sealed net 6 is matched and connected to the upper end of the supporting seat 5. The lower end of the top mesh cover 8 is in contact with the upper surface of the open end of the connecting seat 2. The molecular sieve filling part 7 is located between the top mesh cover 8 and the sealed net 6. The sampling pipe 9 is arranged at the connecting base 1 side corresponding to the upper end position of the top mesh cover 8. The drainage pipe 10 is arranged at the side close to the lower end edge position of the connecting base 1.

[0026] Specifically, the connecting base 1 can be used for supporting and connecting components. The upper end of the connecting seat 2 is provided with an open end. The connecting sleeve 3 can be used for installing connecting vertical pipes 4 of different heights. In use, the height of the connecting vertical pipe 4 can be increased for smaller protein diameters to provide greater pressure and shorten the drainage time. The supporting seat 5 can support the sealed net 6, so that the liquid biological sieve does not flow into the lower end of the sealed net 6. In use, the single-diameter molecular sieve or two molecular sieves with different diameters are filled according to the distribution form of being larger at the lower end and smaller at the upper end to form the molecular sieve filling part 7. The top mesh cover 8 can facilitate the sedimentation of biological sieve particles and reduce the dispersion under the impact of liquid addition, thereby improving the filtering effect. The concentrated liquid after filtration can be discharged through the sampling pipe 9, and the water discharged from the solution is gathered below the sealed net 6 and then discharged through the drainage pipe 10. Compared with the existing beaker test tube assembly structure, the structure is convenient and stable in operation, the filtering speed is increased, and the subsequent cleaning is facilitated, thereby greatly improving the production efficiency and having good practicability.

[0027] Please refer to Figure 2 As another specific embodiment provided by the application, a control valve 11 is further arranged on the sampling pipe 9.

[0028] Specifically, the control valve 11 can control the on-off of the sampling pipe 9, thereby realizing the taking out of the concentrated biological liquid such as protein.

[0029] Please refer to Figure 4 As another specific embodiment provided by the application, a mounting seat 12 is further fixedly connected in an annular array to the lower surface of the connecting base 1.

[0030] Specific said, the installation of the seat 12 is convenient for supporting the connecting base 1 at a certain height, and facilitating flexible operation.

[0031] Please refer to Figure 1 , as another embodiment provided by the application, further comprising a handle 13, the handle 13 is symmetrically fixedly connected to the two side surfaces of the upper end of the connecting seat 2.

[0032] Specific said, the handle 13 is convenient for carrying and holding, improving the stability during lifting and moving, and reducing spilling.

[0033] Please refer to Figure 2 , as another embodiment provided by the application, further comprising a flow buffer plate 14, the flow buffer plate 14 is arranged on the inner wall of the connecting vertical pipe 4 near the upper end, and there is a gap between the edge of the flow buffer plate 14 and the inner wall of the connecting vertical pipe 4.

[0034] Specific said, the flow buffer plate 14 can buffer the added liquid, so that it flows down along the inner wall of the connecting vertical pipe 4, reducing the impact on the bottom and the dispersion at the molecular sieve filling part 7.

[0035] Please refer to Figure 4 , as another embodiment provided by the application, further comprising a scale line 15, the scale line 15 is equidistantly arranged on the outer surface of the connecting vertical pipe 4.

[0036] Specific said, the scale line 15 can be more intuitive to observe the height of the liquid level, so as to facilitate subsequent operation or liquid addition in time.

[0037] Please refer to Figure 4 , as another embodiment provided by the application, further comprising a mounting hole 16, the mounting hole 16 is respectively arranged on the mounting seat 12.

[0038] Specific said, the mounting hole 16 can be firmly connected to the mounting seat 12, so as to connect the mounting seat 12 to different equipment or support planes, and improve the firmness.

[0039] Please refer to Figure 5 , as another embodiment provided by the application, further comprising a sealing gasket 17, the sealing gasket 17 is arranged in the gap between the lower end of the connecting vertical pipe 4 and the upper end of the connecting seat 2.

[0040] Specific said, the sealing gasket 17 can fill and seal the gap between the connecting vertical pipe 4 and the connecting seat 2, which is made of silica gel or other stable elastic material to reduce or avoid affecting the biological solution.

[0041] The embodiments of the present application are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application, and thus: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A drainage device for a protein purification apparatus, characterized by, Include: The connecting base (1), connecting seat (2), connecting sleeve (3), connecting vertical pipe (4), support seat (5), tight net (6), molecular sieve filling part (7), top mesh cover (8), sampling tube (9) and drain pipe (10), the connecting base (1) upper end is matched with connecting seat (2), the upper end of connecting seat (2) is provided with open, connecting sleeve (3) is fixedly connected on the surface of connecting seat (2) open edge position, the inner surface of connecting sleeve (3) is provided with internal thread, the lower end of connecting vertical pipe (4) is provided with external thread, the lower end of connecting vertical pipe (4) is screwed with the inner surface of connecting sleeve (3), the support seat (5) is fixedly connected on the upper surface of connecting base (1) intermediate position, the tight net (6) is matched with connecting in the upper end of support seat (5), the lower end of top mesh cover (8) is in contact with the upper surface of connecting seat (2) opening, the molecular sieve filling part (7) is located between top mesh cover (8) and tight net (6), the sampling tube (9) is set in the corresponding connecting base (1) side of top mesh cover (8) upper end position, the drain pipe (10) is set in the side of connecting base (1) lower end near edge position.

2. The drain device for a protein purification apparatus according to claim 1, characterized by: Still including control valve (11), the control valve (11) is set on sampling tube (9).

3. The drain device for a protein purification apparatus according to claim 1, characterized by: Still including mounting seat (12), the mounting seat (12) annular array is fixedly connected on the lower surface of connecting base (1).

4. The drain device for a protein purification apparatus according to claim 1, wherein: Still including handle (13), the handle (13) is fixedly connected on the both sides of the upper end of connecting seat (2) surface.

5. The drain device for a protein purification apparatus according to claim 1, wherein: Still including slow flow plate (14), the slow flow plate (14) is set on the inner wall of connecting vertical pipe (4) near the upper end, the edge of slow flow plate (14) and the gap between connecting vertical pipe (4) inner wall exists.

6. The drain device for a protein purification apparatus according to claim 1, wherein: Still including scale line (15), the scale line (15) is equidistantly set on the outer surface of connecting vertical pipe (4).

7. The drain device for a protein purification apparatus according to claim 3, characterized by: Still including mounting hole (16), the mounting hole (16) is respectively set through in mounting seat (12).

8. The drain device for a protein purification apparatus according to claim 1, wherein: Still including sealing washer (17), the sealing washer (17) is set in the gap between the lower end of connecting vertical pipe (4) and the upper end of connecting seat (2).

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