Cell wall breaking device for protein extraction

Abstract

The utility model discloses a cell wall breaking device for protein extraction, and relates to the field of ultrasonic cell wall breaking devices. The wall breaking device comprises a device shell and a wall breaking cup, an ultrasonic device is arranged at the top of the device shell, a bearing plate is arranged on the inner side of the device shell, and a longitudinal adjusting assembly is installed on one side of the bearing plate. Due to the meshing relation between the gear and the fixed spur rack and the interaction between the movable spur rack and the gear, the movable plate and the four sets of limiting rods ascend along with the gear, the wall breaking cup is automatically limited, the two steps of alignment and limiting are ingeniously combined into a whole, the operation time is saved, and the working efficiency is improved. In addition, the stability of the wall breaking cup in the ultrasonic action process is ensured, and the overall efficiency of protein breaking extraction is remarkably improved.

Description

Technical Field

[0001] This utility model relates to the field of ultrasonic cell wall breaking devices, specifically a cell wall breaking device for protein extraction. Background Technology

[0002] Cell disruption devices play a crucial role in protein extraction. They disrupt cell walls through physical, chemical, or biological methods, releasing intracellular substances. Ultrasonic cell disruptors and high-pressure homogenizers are two commonly used devices, offering advantages such as high efficiency, speed, and wide applicability, meeting the needs of both laboratory and large-scale production. In existing ultrasonic cell disruption devices, limiting the position of the disruption cup is an essential step to improve the stability of the protein extraction process. Traditionally, the disruption cup is placed on a stage, then a limiting mechanism is used to position it before aligning the ultrasonic probe with the cup. This process is cumbersome and reduces protein extraction efficiency. Therefore, the inventors designed an ultrasonic cell disruption device that reduces and optimizes the limiting step, thereby improving protein extraction efficiency. Utility Model Content

[0003] Based on this, the purpose of this utility model is to provide a cell wall disruption device for protein extraction, so as to solve the technical problem that the limiting steps in the existing ultrasonic cell wall disruption device are cumbersome and affect the protein extraction efficiency.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a cell wall breaking device for protein extraction, comprising a device shell and a cell wall breaking cup. An ultrasonic device is provided on the top of the device shell, and a receiving plate is provided on the inner side of the device shell. A longitudinal adjustment component is installed on one side of the receiving plate. The longitudinal adjustment component includes a lower fixed plate and an upper fixed plate. A limiting mechanism for limiting the cell wall breaking cup is provided below the receiving plate. The limiting mechanism includes a movable plate. A movable straight rack is fixedly connected to one side of the movable plate. A fixed straight rack is fixedly installed between the lower fixed plate and the upper fixed plate. A gear meshes between the movable straight rack and the fixed straight rack. The gear is rotatably connected to the receiving plate through a mounting bracket. Four sets of limiting rods are installed on the movable plate.

[0005] By adopting the above technical solution, the design of the receiving plate and its longitudinal adjustment component on one side allows the height of the blending cup to be flexibly adjusted according to actual needs, ensuring that the ultrasonic rod can accurately act on the cells inside the blending cup.

[0006] Furthermore, the limiting rod is fastened to the movable plate by two mounting nuts, which are used to fine-tune the longitudinal installation height of the limiting rod.

[0007] By adopting the above technical solution, not only is the stability of the limiting rod ensured, but the longitudinal installation height of the limiting rod can also be finely adjusted as needed. This fine-adjustment function is crucial for adapting to different sizes of blending cups and ensuring the optimal distance between the ultrasonic rod and the blending cup.

[0008] Furthermore, the limiting rod passes through the movable plate via the mounting groove, and the limiting rod passes through the receiving plate via the adjusting groove. The mounting groove and the adjusting groove are straight groove structures.

[0009] By adopting the above technical solution, the limiting rod can move with the movement of the movable plate, thereby limiting the movement of the blending cup. At the same time, the limiting rod also passes through the receiving plate through the adjustment groove, which not only provides sufficient room for the limiting rod to move, but also makes the adjustment of the limiting rod more flexible and convenient.

[0010] Furthermore, both the movable and fixed racks pass through the receiving plate via through slots, which are used to provide movable slots for the movable and fixed racks.

[0011] By adopting the above technical solution, the moving and fixed racks can transmit power to the moving plate without interference from the receiving plate. At the same time, the through slot provides sufficient space for the movement of the moving and fixed racks, ensuring the normal operation of the limiting mechanism.

[0012] Furthermore, two sets of guide rods and lead screws are installed between the lower fixed plate and the upper fixed plate. The lead screws are threadedly connected to the receiving plate, and a handwheel is installed at the top of the lead screws.

[0013] By adopting the above technical solution, the two sets of guide rods and lead screws provide stable support and guidance for the receiving plate. The threaded connection between the lead screw and the receiving plate allows the receiving plate to move up and down as the lead screw rotates, thereby adjusting the height of the blending cup. At the same time, the handwheel allows users to easily and conveniently rotate the lead screw, improving the ease of operation.

[0014] Furthermore, the four sets of limiting rods are arranged in a rectangular array, and the outer side of the limiting rods is fitted with a buffer rubber sleeve.

[0015] By adopting the above technical solution, the blending cup is effectively limited and supported in four directions, avoiding shaking or displacement caused by uneven force during the blending process.

[0016] Furthermore, an ultrasonic rod is provided at the bottom of the ultrasonic device, the ultrasonic rod is longitudinally opposite to the blending cup, and a side door is rotatably connected to the front of the device housing, with a handle installed on one side of the side door.

[0017] By adopting the above technical solution, it is ensured that ultrasound can directly and effectively act on the cells inside the cell wall breaking cup, thereby improving the efficiency and effect of cell wall breaking.

[0018] In summary, the present invention has the following main advantages:

[0019] 1. This utility model, through a longitudinal adjustment component and a limiting mechanism, allows users to drive the screw to rotate simply by turning the handwheel, thereby moving the receiving plate and the blending cup placed on it upwards to achieve precise alignment with the ultrasonic bar. This operation simplifies the complex process of traditional alignment and limiting, greatly improving the convenience of operation. At the same time, during the rising of the receiving plate, due to the meshing relationship between the gear and the fixed rack, as well as the interaction between the movable rack and the gear, the movable plate and the four sets of limiting rods rise accordingly, automatically limiting the blending cup. The alignment and limiting steps are cleverly combined into one, which not only saves operation time but also ensures the stability of the blending cup during the ultrasonic process, significantly improving the overall efficiency of protein crushing and extraction.

[0020] 2. By setting up an installation groove and an adjustment groove, this utility model not only provides sufficient space for the movement of the limiting rod on the receiving plate, but also ensures the stability and accuracy of the limiting rod during movement. The straight groove structure design allows the limiting rod to move along a fixed trajectory, which not only improves the ease of operation of the cell wall breaking device, but also enhances its environmental adaptability, enabling the device to adapt to cell wall breaking cups of different sizes and shapes, thereby meeting a wider range of protein crushing and extraction needs. Attached Figure Description

[0021] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0022] Figure 2 This is a schematic diagram of the assembly structure of the longitudinal adjustment component and the limiting mechanism of this utility model;

[0023] Figure 3 This is a cross-sectional structural diagram of the present invention;

[0024] Figure 4 This utility model Figure 3 Enlarged structural diagram at point A in the middle.

[0025] In the diagram: 1. Device housing; 2. Side door; 3. Ultrasonic device; 4. Ultrasonic rod; 5. Receiving plate; 6. Longitudinal adjustment assembly; 601. Lower fixed plate; 602. Upper fixed plate; 603. Lead screw; 604. Guide rod; 605. Handwheel; 7. Limiting mechanism; 701. Movable plate; 702. Movable rack; 703. Fixed rack; 704. Gear; 705. Limiting rod; 706. Mounting nut; 707. Mounting groove; 708. Adjusting groove; 8. Blending cup. Detailed Implementation

[0026] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0027] Example 1:

[0028] A cell wall disruption device for protein extraction, such as Figures 1-4 As shown, the device includes a housing 1 and a blending cup 8. An ultrasonic device 3 is mounted on the top of the housing 1. A receiving plate 5 is located on the inner side of the housing 1. A longitudinal adjustment assembly 6 is installed on one side of the receiving plate 5. The longitudinal adjustment assembly 6 includes a lower fixed plate 601 and an upper fixed plate 602. A limiting mechanism 7 for limiting the blending cup 8 is located below the receiving plate 5. The limiting mechanism 7 includes a movable plate 701. A movable rack 702 is fixedly connected to one side of the movable plate 701. A fixed rack 703 is fixedly installed between the lower fixed plate 601 and the upper fixed plate 602. A gear 70 meshes between the movable rack 702 and the fixed rack 703. 4. Gear 704 is rotatably connected to the receiving plate 5 via the mounting bracket. Four sets of limiting rods 705 are installed on the movable plate 701. The design of the receiving plate 5 and its longitudinal adjustment component 6 on one side allows the height of the cell-wall breaking cup 8 to be flexibly adjusted according to actual needs, ensuring that the ultrasonic rod 4 can accurately act on the cells inside the cell-wall breaking cup 8. In addition, the introduction of the limiting mechanism 7, through the synergistic action of the movable plate 701, the movable rack 702, the fixed rack 703 and the gear 704, achieves stable limiting of the cell-wall breaking cup 8, avoiding large-scale shaking or displacement of the cell-wall breaking cup 8 during the cell-wall breaking process, further improving the cell-wall breaking effect and the efficiency of protein extraction.

[0029] See Figure 2 , Figure 3 , Figure 4The limiting rod 705 is fastened to the movable plate 701 by two mounting nuts 706, which are used to fine-tune the longitudinal installation height of the limiting rod 705. This not only ensures the stability of the limiting rod 705, but also allows the longitudinal installation height of the limiting rod 705 to be finely adjusted as needed. This fine-tuning function is crucial for adapting to different sizes of cell wall breaking cups 8 and ensuring the optimal distance between the ultrasonic rod 4 and the cell wall breaking cup 8. At the same time, by fine-tuning the height of the limiting rod 705, the stability of cell wall breaking can be further optimized, and the purity and yield of protein extraction can be improved.

[0030] Example 2:

[0031] See Figure 2 , Figure 3 , Figure 4 The limiting rod 705 passes through the movable plate 701 via the mounting groove 707 and through the receiving plate 5 via the adjusting groove 708. The mounting groove 707 and the adjusting groove 708 are straight grooves, which allows the limiting rod 705 to move with the movable plate 701, thereby limiting the movement of the blending cup 8. At the same time, the limiting rod 705 also passes through the receiving plate 5 via the adjusting groove 708, which not only provides sufficient space for the limiting rod 705 to move, but also makes the adjustment of the limiting rod 705 more flexible and convenient. The straight groove structure of the mounting groove 707 and the adjusting groove 708 also ensures the stability and accuracy of the limiting rod 705 during movement.

[0032] See Figure 2 , Figure 3 , Figure 4 Both the movable rack 702 and the fixed rack 703 pass through the receiving plate 5 via through slots, providing movement grooves for the movable rack 702 and the fixed rack 703. This prevents the receiving plate 5 from interfering with the movement of the movable rack 702 and the fixed rack 703 when they are transmitting power to the movable plate 701. At the same time, the through slots provide sufficient space for the movement of the movable rack 702 and the fixed rack 703, ensuring the normal operation of the limiting mechanism 7. In addition, the through slots make the structure of the receiving plate 5 simpler and clearer, making it easier for users to install, debug and maintain.

[0033] See Figure 2 , Figure 3Two sets of guide rods 604 and screw rods 603 are installed between the lower fixed plate 601 and the upper fixed plate 602. The screw rods 603 are threadedly connected to the receiving plate 5. A handwheel 605 is installed at the top of the screw rods 603. The two sets of guide rods 604 and screw rods 603 provide stable support and guidance for the receiving plate 5. The threaded connection between the screw rods 603 and the receiving plate 5 allows the receiving plate 5 to move up and down as the screw rods 603 rotate, thereby adjusting the height of the blending cup 8. At the same time, the handwheel 605 allows users to easily and conveniently rotate the screw rods 603, improving the ease of operation. In addition, the presence of the guide rods 604 also ensures the stability and accuracy of the receiving plate 5 during movement, avoiding problems such as poor cell wall breaking effect or damage to the blending cup 8 caused by deviation or shaking.

[0034] See Figure 2 The four sets of limiting rods 705 are arranged in a rectangular array, and the outer side of the limiting rods 705 is covered with a buffer rubber sleeve, which effectively limits and supports the blending cup 8 in four directions, avoiding shaking or displacement caused by uneven force during the blending process. At the same time, the buffer rubber sleeve on the outer side of the limiting rods 705 not only increases the contact area and friction between the limiting rods 705 and the blending cup 8, improving the stability and reliability of the limiting, but also effectively protects the blending cup 8 from the hard impact and wear of the limiting rods 705.

[0035] See Figure 1 , Figure 3 An ultrasonic rod 4 is provided at the bottom of the ultrasonic device 3. The ultrasonic rod 4 is longitudinally opposite to the cell wall blending cup 8. A side door 2 is rotatably connected to the front of the device housing 1, which ensures that the ultrasonic waves can directly and effectively act on the cells in the cell wall blending cup 8, improving the efficiency and effect of cell wall blending. At the same time, the side door 2 rotatably connected to the front of the device housing 1 allows the user to easily and quickly open or close the device housing 1, facilitating the placement and removal of the cell wall blending cup 8, as well as the cleaning and maintenance of the device.

[0036] The implementation principle of this utility model is as follows: First, place the blending cup 8 on the receiving plate 5, and align the blending cup 8 with the ultrasonic rod 4 above it. Then, close the side door 2, and turn the handwheel 605 to rotate the lead screw 603. The lead screw 603 is threadedly connected to the receiving plate 5, causing the receiving plate 5 to move the blending cup 8 upwards until the ultrasonic rod 4 penetrates to a certain depth inside the blending cup 8. Simultaneously, because the gear 704 meshes with the fixed spur rack 703, the gear 704 rotates. The movable spur rack 702 on one side moves upward, driving the movable plate 701 on the other side and the four sets of limiting rods 705 to move upward as well. At this time, the four sets of limiting rods 705 are used to limit the wall-breaking cup 8. In the above operation, the alignment operation between the wall-breaking cup 8 and the ultrasonic rod 4 and the limiting operation of the limiting mechanism 7 on the wall-breaking cup 8 are integrated into one. Only by turning the handwheel, the alignment operation between the wall-breaking cup 8 and the ultrasonic rod 4 and the limiting operation of the limiting mechanism 7 on the wall-breaking cup 8 can be completed, which is beneficial to improving the efficiency of protein crushing and extraction.

[0037] All parts not covered in this utility model are the same as or can be implemented using existing technologies, and will not be described in detail here.

[0038] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. A cell wall disruption device for protein extraction, characterized in that: The device includes a housing (1) and a blending cup (8). An ultrasonic device (3) is provided on the top of the housing (1). A receiving plate (5) is provided on the inner side of the housing (1). A longitudinal adjustment component (6) is installed on one side of the receiving plate (5). The longitudinal adjustment component (6) includes a lower fixing plate (601) and an upper fixing plate (602). A limiting mechanism (7) for limiting the blending cup (8) is provided below the receiving plate (5). The device includes a movable plate (701), a movable rack (702) is fixedly connected to one side of the movable plate (701), a fixed rack (703) is fixedly installed between the lower fixed plate (601) and the upper fixed plate (602), a gear (704) meshes between the movable rack (702) and the fixed rack (703), the gear (704) is rotatably connected to the receiving plate (5) through a mounting bracket, and four sets of limit rods (705) are installed on the movable plate (701).

2. The cell wall disruption device for protein extraction according to claim 1, characterized in that: The limiting rod (705) is fastened to the movable plate (701) by two mounting nuts (706) for fine-tuning the longitudinal installation height of the limiting rod (705).

3. The cell wall disruption device for protein extraction according to claim 1, characterized in that: The limiting rod (705) passes through the movable plate (701) through the mounting groove (707), and the limiting rod (705) passes through the receiving plate (5) through the adjustment groove (708). The mounting groove (707) and the adjustment groove (708) are straight groove structures.

4. The cell wall disruption device for protein extraction according to claim 1, characterized in that: The movable rack (702) and the fixed rack (703) both pass through the receiving plate (5) via through slots to provide movable slots for the movable rack (702) and the fixed rack (703).

5. The cell wall disruption device for protein extraction according to claim 1, characterized in that: Two sets of guide rods (604) and lead screws (603) are installed between the lower fixed plate (601) and the upper fixed plate (602). The lead screws (603) are threadedly connected to the receiving plate (5), and a handwheel (605) is installed at the top of the lead screws (603).

6. The cell wall disruption device for protein extraction according to claim 1, characterized in that: The four sets of limiting rods (705) are arranged in a rectangular array, and the outer side of the limiting rods (705) is fitted with a buffer rubber sleeve.

7. The cell wall disruption device for protein extraction according to claim 1, characterized in that: The ultrasonic device (3) is provided with an ultrasonic rod (4) at its bottom end, and the ultrasonic rod (4) is longitudinally opposite to the wall-breaking cup (8).

8. The cell wall disruption device for protein extraction according to claim 1, characterized in that: The front of the device housing (1) is rotatably connected to a side door (2), and a handle is installed on one side of the side door (2).

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