Recombinant protein constant-temperature storage device

Abstract

The utility model relates to the technical field of constant-temperature culture devices, in particular to a recombinant protein constant-temperature storage device which comprises a constant-temperature culture bin and a transparent vessel, a sealing plate is arranged on one side of the culture bin, a placement plate is mounted in the middle of the culture bin, and a plurality of limiting assemblies are fixedly arranged at the upper end of the placement plate. A containing hole for containing a transparent vessel is formed in the middle of the limiting support, and a limiting suction cup is fixedly arranged at the bottom of the containing hole. After the transparent vessel is placed in the placing hole in the limiting support, the periphery of the transparent vessel is limited, meanwhile, the limiting suction cup at the bottom of the placing hole can suck the bottom of the transparent vessel, and therefore the transparent vessel is further limited.

Description

Technical Field

[0001] This application relates to the field of thermostatic culture device technology, and in particular to a thermostatic preservation device for recombinant proteins. Background Technology

[0002] In the field of biotechnology, the expression and purification of recombinant proteins is an important technique. Recombinant proteins have significant value in many applications, such as biopharmaceuticals, biosensors, and enzyme engineering. To achieve effective expression and purification of recombinant proteins, it is necessary to optimize culture conditions, such as temperature, culture medium composition, and dissolved oxygen concentration.

[0003] In the process of cultivation, recombinant protein is placed in a transparent container and then placed inside a constant temperature chamber for cultivation. When it is necessary to transfer the transparent container, in order to ensure that the temperature of the container does not change, the entire constant temperature chamber is moved. Therefore, in order to prevent the transparent container from tipping over, this application provides a recombinant protein constant temperature preservation device. Utility Model Content

[0004] In view of the shortcomings of the existing technology, the purpose of this application is to provide a recombinant protein isothermal preservation device to solve the technical problems mentioned in the background art.

[0005] The above-mentioned objective of this application is achieved through the following technical solution: a recombinant protein constant temperature preservation device, including a constant temperature culture chamber and a transparent container, a sealing plate is provided on one side of the culture chamber, a placement plate is installed in the middle of the culture chamber, a plurality of limiting components are provided at the upper end of the placement plate for fixing, the limiting components include a limiting bracket fixedly connected to the limiting bracket, a placement hole for placing the transparent container is opened in the middle of the limiting bracket, and a limiting suction cup is fixedly provided at the bottom of the placement hole.

[0006] By adopting the above technical solution, after placing the transparent container into the placement hole in the limiting bracket, the transparent container is limited around its perimeter. At the same time, the limiting suction cup at the bottom of the placement hole will adhere to the bottom of the transparent container, thereby further limiting its position.

[0007] Furthermore, a rotating plate is rotatably provided on one side of the culture chamber, the rotating plate and the sealing plate are rotatably connected, and a locking component is fixedly provided on the side of the sealing plate away from the rotating plate.

[0008] Furthermore, the locking assembly includes a plug-in block fixedly connected to the sealing plate, a locking block fixedly installed on the culture chamber, a plug-in groove matching the plug-in block on the locking block, a locking magnet fixedly installed in the plug-in groove, an iron block fixedly installed at the end of the plug-in block, and an assist groove opened on one side of the sealing plate.

[0009] By adopting the above technical solution, when the sealing plate is closed, the seal is rotated to align the insertion slot on the sealing plate with the insertion slot on the culture chamber, and it is locked by the attraction of the iron block and the locking magnet. At the same time, when opening, the setting of the assist groove can provide a force point for opening the sealing plate.

[0010] Furthermore, a flexible constant temperature plate is fixedly installed on the side of the sealing plate near the culture chamber.

[0011] By adopting the above technical solution, when the sealing plate is closed, the flexible constant temperature plate can block the gaps and ensure that the temperature inside the culture chamber remains constant.

[0012] Furthermore, the placement plate is provided with adjustment frames on both sides, the placement plate is slidably connected to the adjustment frames on both sides, the adjustment frames are fixedly provided with fixing magnets, and the culture chamber is fixedly provided with locking magnet strips for the fixing magnets to attract.

[0013] By adopting the above technical solution, and by sliding the placement plate to both sides of the adjustment frame, all glassware can be removed at once during take-out. At the same time, the setting of fixing magnets and locking magnets improves the connectivity between the placement plate and the culture chamber.

[0014] Furthermore, each side of the adjustment frame is provided with a sliding rod and a threaded rod. The sliding rod and the threaded rod are rotatably connected in the culture chamber. The sliding rod is slidably connected to the adjustment frame, and the threaded rod is threadedly connected to the adjustment frame. Each threaded rod is provided with a drive component at its upper end for driving the threaded rod to rotate.

[0015] By adopting the above technical solution, the drive component rotates, which in turn drives the threaded rod to rotate, thereby moving the threaded adjustment frame up and down. This allows the height between the placement plate and the culture chamber to be changed, enabling the placement of culture vessels of different heights.

[0016] Furthermore, the driving assembly includes a drive motor fixedly connected to the upper end of the culture vessel, and the output end of the drive motor is fixedly connected to a threaded rod.

[0017] By adopting the above technical solution, the two drive motors rotate together, thereby driving the two threaded rods to rotate together, thus moving the adjustment frame.

[0018] Furthermore, the flexible thermostatic plate is made of cork rubber.

[0019] In summary, this application includes the following beneficial technical effects: the limiting components on the placement plate, including a limiting bracket and a limiting suction cup, can secure the transparent vessel from all directions. The limiting bracket prevents the transparent vessel from shaking or tipping over in the culture chamber by limiting it from all sides; while the limiting suction cup further stabilizes the transparent vessel through its suction force at the bottom, ensuring its stability during storage.

[0020] The device's design makes placing and removing transparent containers simple and quick. Users simply place the transparent container into the placement hole of the limiting bracket, and it automatically limits the container's position on all sides and bottom, requiring no additional securing steps. Similarly, removing the container is as easy as gently lifting it. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure in the embodiment;

[0022] Figure 2 yes Figure 1 Sectional view along section line AA.

[0023] Reference numerals: 1. Culture chamber; 11. Placement plate; 12. Fixed magnet; 13. Locking magnet strip; 14. Adjustment frame; 15. Sliding rod; 16. Threaded rod; 17. Drive motor; 18. Limiting bracket; 19. Limiting suction cup; 2. Sealing plate; 21. Flexible constant temperature plate; 22. Insertion block; 23. Iron block; 24. Locking block; 25. Locking magnet; 26. Assist groove; 3. Rotating plate. Detailed Implementation

[0024] The present application will be further described in detail below with reference to the accompanying drawings.

[0025] Example, refer to Figure 1 - Figure 2 A recombinant protein isothermal preservation device includes an isothermal culture chamber 1 and a transparent dish. A sealing plate 2 is provided on one side of the culture chamber 1, and a placement plate 11 is installed in the middle of the culture chamber 1. Multiple sets of limiting components are provided on the upper end of the placement plate 11 for fixed installation. Each limiting component includes a limiting bracket 18 fixedly connected to it. A placement hole for placing the transparent dish is opened in the middle of the limiting bracket 18, and a limiting suction cup 19 is fixedly installed at the bottom of the placement hole. By placing the transparent dish into the placement hole in the limiting bracket 18, the transparent dish is limited around its perimeter. Simultaneously, the limiting suction cup 19 at the bottom of the placement hole adheres to the bottom of the transparent dish, further limiting its position.

[0026] In this embodiment, a rotating plate 3 is rotatably mounted on one side of the culture chamber 1, and the rotating plate 3 is rotatably connected to the sealing plate 2. A locking assembly is fixedly mounted on the side of the sealing plate 2 away from the rotating plate 3. The locking assembly includes a plug-in block 22 fixedly connected to the sealing plate 2, a locking block 24 fixedly mounted on the culture chamber 1, a plug-in groove matching the plug-in block 22 on the locking block 24, a locking magnet 25 fixedly mounted in the plug-in groove, an iron block 23 fixedly mounted at the end of the plug-in block 22, and an assist groove 26 on one side of the sealing plate 2.

[0027] When the sealing plate 2 is closed, the seal is rotated so that the insertion slot on the sealing plate 2 is aligned with the insertion slot on the culture chamber 1. At the same time, it is locked by the attraction of the iron block 23 and the locking magnet 25. When opening, the setting of the assist groove 26 can provide a force point for opening the sealing plate 2.

[0028] In this embodiment, a flexible thermostatic plate 21 is fixedly installed on the side of the sealing plate 2 near the culture chamber 1. When the sealing plate 2 is closed, the flexible thermostatic plate 21 can block gaps and ensure that the temperature inside the culture chamber 1 remains constant.

[0029] In this embodiment, adjustment racks 14 are provided on both sides of the placement plate 11. The placement plate 11 is slidably connected to the adjustment racks 14 on both sides. A fixing magnet 12 is fixedly installed inside the adjustment rack 14, and a locking magnet strip 13 for the fixing magnet 12 to attract is fixedly installed inside the culture chamber 1. By sliding the placement plate 11 to both sides of the adjustment rack 14, all glassware can be taken out at once when removing them. At the same time, the setting of the fixing magnet 12 and the locking magnet 25 improves the connectivity between the placement plate 11 and the culture chamber 1.

[0030] In this embodiment, each side of the adjustment frame 14 is provided with a sliding rod 15 and a threaded rod 16. Both the sliding rod 15 and the threaded rod 16 are rotatably connected to the culture chamber 1. The sliding rod 15 is slidably connected to the adjustment frame 14, and the threaded rod 16 is threadedly connected to the adjustment frame 14. Each threaded rod 16 has a drive assembly at its upper end for rotating. By rotating the drive assembly, the threaded rod 16 rotates, thereby moving the threadedly connected adjustment frame 14 up and down, thus changing the height between the placement plate 11 and the culture chamber 1, allowing for the placement of culture vessels of different heights.

[0031] In this embodiment, the driving assembly includes a drive motor 17 fixedly connected to the upper end of the culture dish, and a threaded rod 16 fixedly connected to the output end of the drive motor 17. The two drive motors 17 rotate together, thereby driving the two threaded rods 16 to rotate together, thus moving the adjusting frame 14. The flexible constant temperature plate 21 is made of cork rubber.

[0032] Specific implementation process: Open the sealing plate 2, and place the transparent containers one by one into the placement holes of the limiting bracket 18, ensuring that the transparent containers are limited by the limiting bracket 18 on all sides and fixed by the limiting suction cup 19 at the bottom. Then, according to the height of the transparent containers, rotate the threaded rod 16 through the drive assembly (drive motor 17) to adjust the position of the placement plate 11 on the adjusting frame 14, ensuring that all transparent containers are in a horizontal and stable state.

[0033] During the sealing and locking phase, gently rotate the sealing plate 2 closer to the culture chamber 1, ensuring that the insertion block 22 is aligned with the insertion slot and the iron block 23 is close to the locking magnet 25. Once the insertion block 22 is fully inserted into the insertion slot, the iron block 23 is attracted by the locking magnet 25, locking the device. Check the locking status to ensure the sealing plate 2 fits tightly against the culture chamber 1 without gaps. Simultaneously, confirm that the flexible thermostatic plate 21 (made of cork rubber) fits tightly against the gap between the culture chamber 1 and the sealing plate 2 to prevent heat loss.

[0034] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A recombinant protein isothermal preservation device, characterized in that, The device includes a constant temperature culture chamber (1) and a transparent container. A sealing plate (2) is provided on one side of the culture chamber (1). A placement plate (11) is installed in the middle of the culture chamber (1). Multiple sets of limiting components are provided on the upper end of the placement plate (11) for fixing. The limiting components include a limiting bracket (18) fixedly connected to the limiting bracket (18). A placement hole for placing the transparent container is opened in the middle of the limiting bracket (18). A limiting suction cup (19) is fixedly provided at the bottom of the placement hole.

2. The recombinant protein isothermal preservation device according to claim 1, characterized in that, The culture chamber (1) is rotatably provided with a rotating plate (3) on one side. The rotating plate (3) and the sealing plate (2) are rotatably connected. A locking component is fixedly provided on the side of the sealing plate (2) away from the rotating plate (3).

3. The recombinant protein isothermal preservation device according to claim 2, characterized in that, The locking assembly includes a plug-in block (22) fixedly connected to the sealing plate (2), a locking block (24) fixedly provided on the culture chamber (1), a plug-in groove matching the plug-in block (22) opened on the locking block (24), a locking magnet (25) fixedly provided in the plug-in groove, an iron block (23) fixedly provided at the end of the plug-in block (22), and an assist groove (26) opened on one side of the sealing plate (2).

4. The recombinant protein isothermal preservation device according to claim 3, characterized in that, A flexible constant temperature plate (21) is fixedly installed on the side of the sealing plate (2) near the culture chamber (1).

5. The recombinant protein isothermal preservation device according to claim 1, characterized in that, The placement plate (11) is provided with adjustment frames (14) on both sides. The placement plate (11) is slidably connected to the adjustment frames (14) on both sides. The adjustment frames (14) are fixedly provided with fixing magnets (12) inside. The culture chamber (1) is fixedly provided with locking magnet strips (13) for the fixing magnets (12) to be attracted.

6. The recombinant protein isothermal preservation device according to claim 5, characterized in that, Each of the adjustment frames (14) on each side is provided with a sliding rod (15) and a threaded rod (16). The sliding rod (15) and the threaded rod (16) are rotatably connected in the culture chamber (1). The sliding rod (15) and the adjustment frame (14) are slidably connected. The threaded rod (16) and the adjustment frame (14) are threadedly connected. Each threaded rod (16) is provided with a drive assembly at its upper end for driving the threaded rod (16) to rotate.

7. The recombinant protein isothermal preservation device according to claim 6, characterized in that, The drive assembly includes a drive motor (17) fixedly connected to the upper end of the culture vessel, and the output end of the drive motor (17) is fixedly connected to a threaded rod (16).

8. The recombinant protein isothermal preservation device according to claim 4, characterized in that, The flexible constant temperature plate (21) is made of cork rubber.

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