Detachable 24-hole nucleic acid purification kit
By designing a detachable 24-well nucleic acid purification kit, the problem of existing kits being unable to be installed on automated instruments was solved, enabling high-efficiency, high-throughput purification in forensic nucleic acid testing and improving purification efficiency.
Patent Information
- Application Number
- CN202422843743.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-21
- Publication Date
- 2026-01-13
- Estimated Expiration
- 2034-11-21
AI Technical Summary
Existing nucleic acid purification kits cannot be installed on automated instruments for forensic nucleic acid testing, thus preventing the achievement of efficient high-throughput purification.
A detachable 24-well nucleic acid purification kit was designed, including a first well plate and a second well plate, with purification tubes and collection tubes fixed on their respective well plates. It can be used on automated nucleic acid detection instruments to achieve high-throughput purification column-based nucleic acid extraction.
This technology enables efficient, high-throughput nucleic acid extraction using a purification column on automated nucleic acid testing instruments, thereby improving purification efficiency.
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Figure CN223793109U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of DNA detection tool technology, specifically a detachable 24-well nucleic acid purification kit. Background Technology
[0002] In the field of forensic DNA testing, samples often need to be filtered and purified. Nucleic acid separation refers to separating nucleic acids from biological macromolecules such as proteins, polysaccharides, and fats. At present, purification column centrifugation is usually used to separate nucleic acids and proteins of different specific gravities to achieve the purpose of purification.
[0003] The existing technology has the following defects and shortcomings:
[0004] Current nucleic acid purification kits are single-well kits, which cannot be installed on automated instruments for forensic nucleic acid detection. Therefore, a detachable 24-well nucleic acid purification kit is proposed. Utility Model Content
[0005] To address the shortcomings of existing technologies, this invention provides a detachable 24-well nucleic acid purification kit, which solves the existing problems.
[0006] To achieve the above objectives, this utility model is implemented through the following technical solution: it includes a first well plate and a second well plate, wherein a purification tube is disposed inside the first well plate and a first connecting part is fixedly connected to the top end of the purification tube, and a collection tube is disposed inside the second well plate and a second connecting part is fixedly connected to the top end of the collection tube.
[0007] Preferably, the first connecting part is connected to the hole in the first perforated plate.
[0008] Preferably, the second connecting part is connected to the hole in the second perforated plate.
[0009] Preferably, the diameter of the purification tube is smaller than the diameter of the collection tube, and the purification tube is located inside the collection tube.
[0010] Preferably, a pressure ring is fixedly connected to the bottom of the inner part of the purification tube.
[0011] Beneficial effects
[0012] This invention provides a detachable 24-well nucleic acid purification kit. Compared with the prior art, it has the following advantages:
[0013] 1. This detachable 24-well nucleic acid purification kit is equipped with a first well plate, a second well plate, a purification tube, and a collection tube. After fixing the purification tube to the first well plate and the collection tube to the second well plate, it can be placed on an automated nucleic acid detection instrument. The instrument only needs to place the first well plate into the second well plate to perform high-throughput purification column nucleic acid extraction. It can not only be installed on automated nucleic acid detection instruments, but also greatly improves efficiency. Attached Figure Description
[0014] Figure 1 This is a partial exploded view of the structure of this utility model;
[0015] Figure 2 This is a schematic diagram of the structure of the first and second perforated plates of this utility model after they are combined.
[0016] In the figure: 1. First well plate; 2. Second well plate; 3. Purification tube; 4. First connecting part; 5. Pressure ring; 6. Collection tube; 7. Second connecting part. Detailed Implementation
[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0018] Please see Figure 1-2 This utility model provides two technical solutions:
[0019] Example 1:
[0020] A detachable 24-well nucleic acid purification kit includes a first well plate 1 and a second well plate 2. The first well plate 1 has a purification tube 3 inside, and a first connecting part 4 is fixedly connected to the top of the purification tube 3. The second well plate 2 has a collection tube 6 inside, and a second connecting part 7 is fixedly connected to the top of the collection tube 6.
[0021] Example 2:
[0022] A detachable 24-well nucleic acid purification kit includes a first well plate 1 and a second well plate 2. A purification tube 3 is disposed inside the first well plate 1. A pressure ring 5 is fixedly connected to the bottom of the purification tube 3 for fixing and compressing a filter membrane. A first connecting part 4 is fixedly connected to the top of the purification tube 3, and the first connecting part 4 is interconnected with the holes of the first well plate 1, thus fixing the purification tube 3 onto the first well plate 1. A collection tube 6 is disposed inside the second well plate 2. A second connecting part 7 is fixedly connected to the top of the collection tube 6, and the second connecting part 7 is interconnected with the holes of the second well plate 2, thus fixing the collection tube 6 onto the second well plate 2. The diameter of the purification tube 3 is smaller than the diameter of the collection tube 6, and the purification tube 3 is located inside the collection tube 6, enabling high-throughput purification column-based nucleic acid extraction.
[0023] 1. Fill the bottom layer of the purification tube 3 with nucleic acid purification membrane, and fill the top layer of the membrane with pressure ring 5 to prevent liquid from leaking through the membrane.
[0024] 2. The filled purification tube 3 is placed in the first well plate 1 and fixed to obtain the purification plate.
[0025] 3. The collection tube 6 is placed in the second perforated plate 2 and fixed to obtain the collection plate.
[0026] 4. Place the purification plate above the collection plate so that the purification tube 3 enters the collection tube 6, and then perform high-throughput purification column nucleic acid extraction.
[0027] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.
[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.
[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A detachable 24-well nucleic acid purification kit, comprising a first well plate (1) and a second well plate (2), characterized in that: The first well plate (1) is provided with a purification tube (3), and the top end of the purification tube (3) is fixedly connected to a first connecting part (4). The second well plate (2) is provided with a collection tube (6), and the top end of the collection tube (6) is fixedly connected to a second connecting part (7).
2. The detachable 24-well nucleic acid purification kit according to claim 1, characterized in that: The first connecting part (4) is connected to the hole of the first perforated plate (1).
3. The detachable 24-well nucleic acid purification kit according to claim 1, characterized in that: The second connecting part (7) is connected to the hole of the second perforated plate (2).
4. The detachable 24-well nucleic acid purification kit according to claim 1, characterized in that: The diameter of the purification tube (3) is smaller than the diameter of the collection tube (6), and the purification tube (3) is located inside the collection tube (6).
5. A detachable 24-well nucleic acid purification kit according to claim 1, characterized in that: A pressure ring (5) is fixedly connected to the bottom of the inner part of the purification tube (3).