PCR centrifugal tube

Abstract

The utility model discloses a PCR centrifuge tube which comprises a tube body and a tube cap in threaded connection with the side wall of the port of the tube body, and further comprises a mounting tube arranged at the port of the tube body, the two ends of the mounting tube are fixedly connected with conical sleeves respectively, the conical large ends of the two conical sleeves are arranged away from each other and are connected with the inner wall of the tube body, and the conical large ends of the two conical sleeves are connected with the mounting tube. The pipe cap is provided with a plugging assembly used for plugging the mounting pipe. According to the PCR centrifugal tube disclosed by the utility model, through the arrangement of the plugging assembly, under the matching action of the extruding assembly and the connecting assembly, the tube opening can be plugged without increasing the thread screwing force between the tube cap and the tube body, so that the risk that the tube body and the tube cap are damaged and deformed due to the overlarge thread screwing force is reduced, and meanwhile, the tube opening is prevented from being blocked. And the convenience of connection and disassembly between the pipe cap and the pipe body is also improved, so that the probability that liquid in the pipe is splashed out when the pipe body and the pipe cap are separated is further reduced.

Description

Technical Field

[0001] This utility model relates to the field of PCR centrifuge tube technology, specifically a PCR centrifuge tube. Background Technology

[0002] PCR centrifuge tubes are tools used to store and separate samples in polymerase chain reaction (PCR) experiments. Existing PCR centrifuge tubes mainly consist of a tube body and a cap that is threaded to the end of the tube body. A sealing ring is provided inside the cap to ensure the airtight connection with the tube body.

[0003] Since the sealing performance of the sealing ring is determined by the degree to which it abuts against the end face of the tube after being compressed and deformed, in actual use, it is necessary to increase the thread engagement force between the tube body and the cap to cause the sealing ring to undergo elastic deformation. This deformation allows the sealing ring to fit tightly against the opening edge of the tube body, thereby filling the tiny gap between the tube body and the sealing ring, preventing liquid leakage from the tube, and also preventing external contaminants from entering the tube. However, if the thread engagement force between the cap and the tube body is too large, the tube body and the cap will be subjected to greater pressure, which can easily cause cracks or deformation in the threaded part of the tube body and stripping of the threads in the cap. As a result, over long-term use, the cap may not be able to be screwed on or tightened properly, thus losing its sealing function. At the same time, excessive engagement force makes the cap difficult to open, especially when the experimenter is wearing latex gloves. This not only wastes the experimenter's time, but also risks that the liquid inside the tube may splash out during forced opening, contaminating the experimental environment or causing sample loss.

[0004] Therefore, there is an urgent need for a PCR centrifuge tube to solve the above problems. Utility Model Content

[0005] To achieve the above objectives, the present invention provides the following technical solution: a PCR centrifuge tube, comprising a tube body and a cap threaded to the side wall of the tube body port, and an installation tube disposed at the tube body port, wherein conical sleeves are fixedly connected to both ends of the installation tube, the large conical ends of the two conical sleeves are disposed far apart from each other and connected to the inner wall of the tube body, and the cap is provided with a sealing component for sealing the installation tube.

[0006] The sealing assembly includes a circular plate slidably connected to the cap, a sealing ball fixedly connected to the side of the circular plate near the pipe body, and a compression assembly for compressing the sealing ball.

[0007] The diameter of the sealing ball is larger than the inner diameter of the installation tube.

[0008] The extrusion assembly includes an extrusion tube disposed on the tube cap, an extrusion rod slidably connected to the extrusion tube, one end of the extrusion rod being connected to the side of the circular plate away from the sealing ball, the end of the extrusion rod away from the circular plate being located inside the extrusion tube and fixedly connected to an extrusion plate, a spring being fixedly connected to the side of the extrusion plate away from the extrusion rod, the end of the spring away from the extrusion plate being connected to the bottom wall of the extrusion tube, and the end of the extrusion tube away from the circular plate being connected to the tube cap through a connecting assembly.

[0009] The extrusion assembly is provided in multiple ways, and each extrusion assembly is arranged in a ring array.

[0010] The connecting assembly includes a connecting hole opened on the side of the cap near the extrusion tube, and a connecting plate is rotatably connected to the connecting hole. The end of each extrusion tube away from the circular plate is connected to the connecting plate.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] This invention relates to a PCR centrifuge tube. Through the design of a sealing component, the tube opening can be sealed without increasing the thread engagement force between the cap and the tube body, thanks to the combined action of the squeezing and connecting components. This achieves sealing at the tube opening, reducing the risk of sample leakage or external air entering the tube and contaminating the sample. It also reduces the risk of damage or deformation to the tube body and cap due to excessive thread engagement force. Furthermore, it improves the ease of connection and disassembly between the cap and the tube body, further reducing the probability of liquid splashing out when the tube body and cap are separated. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a schematic diagram of the internal structure of the tube body and cap of this utility model;

[0015] Figure 3 This is a schematic diagram of the internal structure of the extrusion assembly of this utility model;

[0016] Figure 4 This is a schematic diagram of the mounting tube and two conical sleeves of this utility model.

[0017] In the diagram: 101, pipe body; 102, pipe cap; 2, installation pipe; 3, conical sleeve; 401, round plate; 402, sealing ball; 501, extrusion pipe; 502, extrusion rod; 503, extrusion plate; 504, spring; 601, connecting hole; 602, connecting plate. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Example 1

[0020] Please see Figures 1-4 The PCR centrifuge tube shown in the figure includes a tube body 101 and a cap 102 threaded to the side wall of the port of the tube body 101. It also includes an installation tube 2 set at the opening of the tube body 101. Conical sleeves 3 are fixedly connected to both ends of the installation tube 2. The large conical ends of the two conical sleeves 3 are set far apart from each other and connected to the inner wall of the tube body 101. The cap 102 is provided with a sealing component for sealing the installation tube 2.

[0021] The plugging assembly includes a circular plate 401 slidably connected to the cap 102, and a plugging ball 402 is fixedly connected to the side of the circular plate 401 near the tube body 101. The cap 102 is provided with a compression assembly for compressing the plugging ball 402.

[0022] It should be noted that, through the design of the sealing component, the sealing of the tube opening can be achieved without increasing the thread engagement force between the cap 102 and the tube body 101, thanks to the combined action of the squeezing component and the connecting component. This not only seals the tube opening of the tube body 101, reducing the risk of sample leakage or external air entering the tube body 101 and contaminating the sample, but also reduces the risk of damage or deformation to the tube body 101 and cap 102 due to excessive thread engagement force. Furthermore, it improves the ease of connection and disassembly between the cap 102 and the tube body 101, thereby further reducing the probability of liquid splashing out of the tube when the tube body 101 and cap 102 are separated.

[0023] Please see Figure 2 The diameter of the sealing ball 402 in the diagram is larger than the inner diameter of the mounting tube 2;

[0024] It should be noted here that by making the diameter of the sealing ball 402 larger than the inner diameter of the installation tube 2, the tightness of the sealing ball 402 in sealing the passage of the installation tube 2 can be ensured.

[0025] Please see Figure 3The extrusion assembly shown in the figure includes an extrusion tube 501 disposed on the tube cap 102. An extrusion rod 502 is slidably connected to the extrusion tube 501. One end of the extrusion rod 502 is connected to the side of the circular plate 401 away from the sealing ball 402. The end of the extrusion rod 502 away from the circular plate 401 is located inside the extrusion tube 501 and is fixedly connected to an extrusion plate 503. A spring 504 is fixedly connected to the side of the extrusion plate 503 away from the extrusion rod 502. The end of the spring 504 away from the extrusion plate 503 is connected to the bottom wall of the extrusion tube 501. The end of the extrusion tube 501 away from the circular plate 401 is connected to the tube cap 102 through a connecting assembly.

[0026] It should be noted that the compression component is used to push the sealing ball 402 against the inner wall of the conical sleeve 3. At the same time, when used in a certain temperature environment, the compression force on the sealing ball 402 can be increased by increasing the amount of screwing on the cap 102. This allows for flexible adjustment of the compression force on the sealing ball 402 according to the actual usage environment, thereby improving the flexibility of sealing the pipe opening of the pipe body 101.

[0027] Please see Figure 3 The diagram shows multiple extrusion components arranged in a circular array.

[0028] It should be noted that multiple extrusion components arranged in a ring array can provide balanced and continuous stable extrusion force to the sealing ball 402.

[0029] Working principle: When using this PCR centrifuge tube, first use a micropipette to add the reagents and samples to tube 101 in the calculated volumes. During the addition process, pay attention to the correct use of the pipette to ensure accurate liquid volume and avoid generating air bubbles. If air bubbles are generated, they may affect the actual volume of the reaction system, thus affecting the accuracy of the reaction. Also, when adding liquid, the pipette tip should be inserted vertically to the bottom of tube 101, and the liquid should be released slowly to prevent splashing.

[0030] After the sample is added into the tube body 101, the cap 102 can be screwed onto the opening of the tube body 101. During the screwing of the cap 102, the sealing ball 402 on one side of the circular plate 401 will move inside the tube body 101. As the cap 102 is continuously screwed on, the sealing ball 402 will abut against the inner wall of the conical sleeve 3 on one side of the mounting tube 2. After the sealing ball 402 abuts against the inner wall of the conical sleeve 3, the cap 102 is screwed on further. Under the squeezing action of the squeezing component, the sealing ball 402 abuts tightly against the inner wall of the conical sleeve 3. Thus, under the tight abutting action of the sealing ball 402 against the inner wall of the conical sleeve 3, the tube body is sealed. The sealing at the tube opening 101 reduces the risk of sample leakage or contamination from external air entering the tube body 101. Furthermore, the sealing ball 402 achieves sealing at the tube opening 101 through the squeezing action of the squeezing component. Therefore, sealing the tube opening can be achieved without increasing the thread engagement force between the cap 102 and the tube body 101. This reduces the risk of damage and deformation to the tube body 101 and cap 102 due to excessive thread engagement force, while improving the ease of connection and disassembly between the cap 102 and the tube body 101. This further reduces the probability of liquid splashing out of the tube when the tube body 101 and cap 102 are separated.

[0031] Example 2

[0032] Please see Figure 3 This embodiment further illustrates Example 1. The connecting component shown in the figure includes a connecting hole 601 opened on the side of the cap 102 near the extrusion tube 501. The connecting hole 601 is rotatably connected to a connecting plate 602. The end of each extrusion tube 501 away from the circular plate 401 is connected to the connecting plate 602.

[0033] It should be noted here that the connection components are designed to prevent the sealing ball 402 from being affected during the thread engagement of the cap 102, thereby reducing the friction between the sealing ball 402 and the tapered sleeve 3.

[0034] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A PCR centrifuge tube, comprising: The pipe body (101) and the pipe cap (102) threadedly connected to the side wall of the port of the pipe body (101); Its characteristic is that it further includes: An installation pipe (2) is installed at the opening of the pipe body (101). Two ends of the installation pipe (2) are respectively fixedly connected to conical sleeves (3). The large conical ends of the two conical sleeves (3) are set far apart from each other and connected to the inner wall of the pipe body (101). The pipe cap (102) is provided with a sealing component for sealing the installation pipe (2).

2. A PCR centrifuge tube according to claim 1, characterized in that: The sealing assembly includes a circular plate (401) slidably connected to the cap (102), and a sealing ball (402) is fixedly connected to the side of the circular plate (401) near the tube body (101). The cap (102) is provided with a compression assembly for compressing the sealing ball (402).

3. A PCR centrifuge tube according to claim 2, characterized in that: The diameter of the sealing ball (402) is larger than the inner diameter of the mounting tube (2).

4. A PCR centrifuge tube according to claim 3, characterized in that: The extrusion assembly includes an extrusion tube (501) disposed on the cap (102). The extrusion tube (501) is slidably connected to an extrusion rod (502). One end of the extrusion rod (502) is connected to the side of the circular plate (401) away from the sealing ball (402). The end of the extrusion rod (502) away from the circular plate (401) is located inside the extrusion tube (501) and is fixedly connected to an extrusion plate (503). A spring (504) is fixedly connected to the side of the extrusion plate (503) away from the extrusion rod (502). The end of the spring (504) away from the extrusion plate (503) is connected to the bottom wall of the extrusion tube (501). The end of the extrusion tube (501) away from the circular plate (401) is connected to the cap (102) through a connecting assembly.

5. A PCR centrifuge tube according to claim 4, characterized in that: The extrusion assembly is provided in multiple ways, and each extrusion assembly is arranged in a ring array.

6. A PCR centrifuge tube according to claim 5, characterized in that: The connecting assembly includes a connecting hole (601) opened on the side of the cap (102) near the extrusion tube (501), the connecting hole (601) is rotatably connected to a connecting plate (602), and the end of each extrusion tube (501) away from the circular plate (401) is connected to the connecting plate (602).

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