Spectrum-based device for measuring organisms in water

Abstract

The utility model relates to the technical field of microbiological determination, in particular to a spectrum-based device for determining organisms in water. The device can automatically clean the dirt of the lens at the sight glass barrel, does not need manual intervention, is suitable for remote sea water quality monitoring, eliminates the influence of dirt attachments on the throughput of a light source, and improves the detection precision of microorganisms in water; comprising an instrument shell, a signal acquisition circuit board arranged in the shell and a photoelectric detection unit connected with the shell, the photoelectric detection unit is electrically connected with the signal acquisition circuit board, and the photoelectric detection unit comprises a sight glass barrel and a photoelectric detection assembly arranged in the sight glass barrel; the lens cleaning mechanism comprises an external thread barrel rotationally mounted on the sight glass barrel, an internal thread ring screwed on the external thread barrel, a driving motor fixedly mounted outside the sight glass barrel, and a lens cleaning part mounted at the end part of the sight glass barrel; and the lens cleaning mechanism comprises an external thread barrel rotationally mounted on the sight glass barrel, an internal thread ring screwed on the external thread barrel, a driving motor fixedly mounted outside the sight glass barrel and a lens cleaning part mounted at the end part of the sight glass barrel.

Description

Technical Field

[0001] This utility model relates to the technical field of microbial assay, and in particular to a spectroscopic aquatic biological assay device. Background Technology

[0002] As is well known, the cell structure and composition of microorganisms form specific spectra, known as fingerprint spectra. Therefore, information about microorganisms can be obtained based on their light absorption characteristics, enabling microbial detection. Obtaining the common fingerprint spectrum of marine microorganisms is crucial for the detection of total microbial mass in seawater. Clearly defining the total microbial population in seawater is a prerequisite for obtaining the common fingerprint spectrum of marine microorganisms. Existing online spectrometers include an instrument housing, a signal acquisition circuit built into the housing, and a photoelectric detection unit integrated with the housing. The photoelectric detection unit includes a sight glass and a photoelectric detection component built into the sight glass. Based on the fluorescence properties of the analyte, the sight glass is directly immersed in the water. The photoelectric detection component excites a light source to emit light of a certain wavelength, which illuminates the water sample. The analyte in the sample is excited and emits fluorescence with a longer wavelength than the excitation light. The fluorescence intensity has a linear relationship with the concentration of the analyte within a certain range. The emitted fluorescence is received by the photoelectric detection component and generates an electrical signal. The content of the analyte in the water is calculated based on the strength of the electrical signal.

[0003] However, when implementing this device, the following defects were found: when the sight tube is immersed in water sample for a long time, dirt easily accumulates on its lens. This dirt affects the passage of light and thus affects the detection structure; the lens needs to be cleaned periodically. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a spectrum-based aquatic biological assay device that can automatically clean lens contaminants at the viewing tube without manual intervention. This device is suitable for remote marine water quality monitoring, eliminates the influence of contaminants on the light source's transmission, and improves the accuracy of aquatic microbial detection.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, this utility model provides the following technical solution: a spectrum-based aquatic bioassay device, comprising an instrument housing, a signal acquisition circuit board built into the housing, and a photoelectric detection unit connected to the housing. The photoelectric detection unit is electrically connected to the signal acquisition circuit board. The photoelectric detection unit includes a sight glass tube and a photoelectric detection component built into the sight glass tube. It also includes a lens cleaning mechanism mounted on the sight glass tube. The lens cleaning mechanism includes an externally threaded cylinder rotatably mounted on the sight glass tube, an internally threaded ring screwed onto the externally threaded cylinder, a drive motor fixedly mounted outside the sight glass tube, and a [missing information - likely a component or component]. A lens cleaning component is located at the end of the sight tube. The output end of the drive motor is connected to the externally threaded cylinder. The outer wall of the externally threaded cylinder has a threaded portion and a smooth portion. The lens cleaning component includes two cleaning brushes hinged to the end of the externally threaded cylinder, a drive ring that slides radially along the smooth portion of the externally threaded cylinder, and two transmission rods. One end of each transmission rod is hinged to the end of the cleaning brush away from the center of the externally threaded cylinder, and the other end of each transmission rod is hinged to the drive ring. Multiple circumferentially distributed springs are installed between the drive ring and the end of the externally threaded cylinder. A guide rod is fixedly installed on the sight tube, and the internally threaded ring is slidably installed on the guide rod. Furthermore, the drive motor provides power for the rotation of the externally threaded cylinder through a synchronous belt or transmission gear. The smooth portion is adjacent to the lens end of the sight tube; the two cleaning brushes are symmetrically arranged at the lens end of the sight tube; one end of each spring is fixedly connected to the drive ring, and the other end of each spring is fixedly connected to the end of the externally threaded cylinder.

[0008] As a preferred embodiment, a tension spring is fitted onto the guide rod, one end of which is fixedly connected to an internal threaded ring, and the other end of which is fixedly connected to the end of the sight tube away from the lens cleaning mechanism.

[0009] As a preferred embodiment, a plurality of balls are rotatably mounted on the end of the internal threaded ring that contacts the drive ring, and the internal threaded ring and the drive ring are in contact through the rotation of the balls.

[0010] As a preferred embodiment, the housing is equipped with a connecting lifting ring.

[0011] As a preferred embodiment, it also includes an intermediate connector, through which the outer shell is detachably connected to the sight tube, and the lens cleaning mechanism is installed at the intermediate connector; furthermore, the outer shell and the sight tube are threaded or socketed to the intermediate connector; sealing and waterproof components such as sealing rings can be added at the connection between the intermediate connector and the outer shell and the sight tube, and the lens cleaning mechanism.

[0012] As a preferred embodiment, the end of the guide rod is fitted with an anti-detachment cap.

[0013] As a preferred embodiment, the photoelectric detection component includes at least a light source, a light guide column, a receiving filter, and a detector; furthermore, the light source may be an LED lamp or a pulsed xenon lamp.

[0014] (III) Beneficial Effects

[0015] Compared with the prior art, this utility model provides a spectrum-based aquatic biological detection device with the following beneficial effects: In its natural state, under the action of the spring, the transmission rod and the cleaning brush are both parallel to the central axis of the sight glass tube, and the cleaning brush is detached from the outer wall of the sight glass tube. By starting the drive motor, the drive motor drives the external threaded cylinder to rotate. After the thread transmission, the internal threaded ring moves along the guide rod towards the lens side of the sight glass tube. When the internal threaded ring pushes the drive ring to move, after the transmission rod, the cleaning brush rotates around the hinge point until the cleaning brush is in close contact with the lens on the sight glass tube. At this time, the internal threaded ring is located at the smooth part of the external threaded cylinder and stops moving. The external threaded cylinder continues to rotate, and the cleaning brush cleans the lens of the sight glass tube. It can automatically clean the lens dirt on the sight glass tube without manual intervention. It is suitable for remote marine water quality monitoring, eliminates the influence of dirt and debris on the light source transmission, and improves the accuracy of aquatic microbial detection. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0017] Figure 2 This is a schematic diagram of the bottom planar structure of this utility model;

[0018] Figure 3 This is the utility model Figure 2 Schematic diagram of the cross-sectional structure at point AA;

[0019] Figure 4 This is the utility model Figure 3 A magnified schematic diagram of the structure at point B in the middle;

[0020] Figure 5 This is a three-dimensional structural diagram of the present invention from another perspective;

[0021] Figure 6 This is a schematic diagram of the optical path of the photoelectric detection component of this utility model;

[0022] The following components are labeled in the attached diagram: 1. Housing; 2. Signal acquisition circuit board; 3. Sight glass tube; 4. External threaded cylinder; 5. Internal threaded ring; 6. Drive motor; 7. Threaded part; 8. Smooth part; 9. Cleaning brush; 10. Drive ring; 11. Transmission rod; 12. Spring; 13. Guide rod; 14. Tension spring; 15. Ball bearing; 16. Connecting ring; 17. Intermediate connector; 18. Anti-detachment seal; 19. Light source; 20. Light guide column; 21. Receiving filter; 22. Detector. Detailed Implementation

[0023] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of the present invention.

[0024] Example 1

[0025] Please refer to Figure 1-4 The spectral-based aquatic bioassay device specifically includes: an instrument housing 1, a signal acquisition circuit board 2 built into the housing 1, and a photoelectric detection unit connected to the housing 1. The photoelectric detection unit is electrically connected to the signal acquisition circuit board 2. The photoelectric detection unit includes a sight glass tube 3 and a photoelectric detection component built into the sight glass tube 3. It also includes a lens cleaning mechanism mounted on the sight glass tube 3. The lens cleaning mechanism includes an external threaded cylinder 4 rotatably mounted on the sight glass tube 3, an internal threaded ring 5 screwed onto the external threaded cylinder 4, a drive motor 6 fixedly mounted outside the sight glass tube 3, and a lens cleaning component mounted at the end of the sight glass tube 3. The output end of the machine 6 is connected to the external threaded cylinder 4. The outer wall of the external threaded cylinder 4 is provided with a threaded part 7 and a smooth part 8. The lens cleaning component includes two cleaning brushes 9 hinged to the ends of the external threaded cylinder 4, a drive ring 10 that slides radially along the smooth part 8 of the external threaded cylinder 4, and two transmission rods 11. One end of each transmission rod 11 is hinged to the end of the cleaning brush 9 away from the center of the external threaded cylinder 4, and the other end of each transmission rod 11 is hinged to the drive ring 10. Multiple circumferentially distributed springs 12 are installed between the drive ring 10 and the end of the external threaded cylinder 4. A guide rod 13 is fixedly installed on the sight glass cylinder 3, and the internal threaded ring 5 is slidably installed on the guide rod 13. Furthermore, the drive motor 6 provides power for the rotation of the external threaded cylinder 4 through a synchronous belt or transmission gear, the smooth part 8 is near the lens end of the sight tube 3; two cleaning brushes 9 are symmetrically arranged at the lens of the sight tube 3; one end of the spring 12 is fixedly connected to the drive ring 10, and the other end of the spring 12 is fixedly connected to the end of the external threaded cylinder 4; it should be noted that the drive motor 6 adopts a commercially available and commonly known underwater motor.

[0026] For details, please refer to Figure 6 The photoelectric detection component includes at least a light source 19, a light guide column 20, a receiving filter 21, and a detector 22; furthermore, the light source 19 can be an LED lamp or a pulsed xenon lamp.

[0027] The photoelectric detection unit is a common, known product on the market. Its specific structure and principle can be found in the FLUORAT-02 fluorescence analyzer and the XHOIL-91A water quality and petroleum automatic monitoring instrument, as detailed below:

[0028] A. The principle of using fluorescence method to determine chlorophyll in water:

[0029] The measuring device is directly immersed in water. The light source 19 emits 470nm light to illuminate the phytoplankton in the water body being tested. After the chlorophyll a in the phytoplankton is excited, it emits fluorescence with a longer wavelength than the excitation light (center wavelength 670nm). The fluorescence intensity has a linear relationship with the concentration of chlorophyll within a certain range. The emitted fluorescence is received by the detector 22 (silicon photocell) and generates an electrical signal. The content of chlorophyll a in the water body being tested is calculated based on the strength of the electrical signal.

[0030] B. The principle of using fluorescence method to determine oil in water:

[0031] The miniature probe is directly immersed in water. The light source 19 emits 254nm light to illuminate the water body being tested. After the oil in the water is excited, it emits fluorescence with a longer wavelength than the excitation light (center wavelength 360nm). The fluorescence intensity has a linear relationship with the concentration of oil in the water within a certain range. The emitted fluorescence is received by the detector 22 (silicon photocell) and generates an electrical signal. The content of oil in the water body being tested is calculated based on the strength of the electrical signal.

[0032] The different excitation and emission wavelengths of oil and chlorophyll in water allow for the creation of online oil and chlorophyll analyzers, respectively, by employing excitation light sources 19 and receiving filters 21 of corresponding wavelengths. This principle can also be used to measure other organisms in water.

[0033] The detection principle of the fluorescence signal is as follows: the detector 22 converts the modulated fluorescence signal into a current signal and processes the electrical signal. The current signal is converted into a sinusoidal fluorescence signal through DC blocking, I / V conversion, and bandpass filtering. After AC amplification, full-wave rectification, and filtering, the fluorescence signal is finally obtained. The signal acquisition circuit board 2 receives and transmits the fluorescence signal. The operator can obtain the required data by receiving and reading the signal through the corresponding signal processor.

[0034] The above principles are merely a further explanation of the photoelectric detection unit and the signal acquisition circuit, and should not be construed as limiting the scope of protection of this case. This case does not further elaborate on or limit the structure and principle of the photoelectric detection unit and the signal acquisition circuit. Any photoelectric detection unit and signal acquisition circuit capable of performing spectral measurements of microorganisms in water can be applied to this case.

[0035] For details, please refer to Figure 1 or Figure 3 A tension spring 14 is fitted on the guide rod 13. One end of the tension spring 14 is fixedly connected to the internal threaded ring 5, and the other end of the tension spring 14 is fixedly connected to the end of the sight tube 3 away from the lens cleaning mechanism.

[0036] Please refer to Figure 4 Multiple balls 15 are rotatably mounted on one end of the internal threaded ring 5 that contacts the drive ring 10, and the internal threaded ring 5 and the drive ring 10 make contact through the balls 15.

[0037] Please refer to Figure 1 or Figure 5 A connecting ring 16 is installed on the outer casing 1.

[0038] In the spectral-based aquatic organism detection device provided in this embodiment, after the sight glass lens is cleaned, the drive motor 6 drives the external threaded cylinder 4 to reverse. Under the action of the tension spring 14, the internal threaded ring 5 can be moved towards the threaded part 7. The internal threaded ring 5 is re-screwed onto the threaded part 7 of the external threaded cylinder 4. After the spring 12 extends, the drive ring 10 returns to its original position, and the cleaning brush 9 disengages from the lens of the sight glass tube 3, avoiding interference between the cleaning brush 9 and the light source 19 passing through the lens of the sight glass tube 3. The ball bearing 15 enables rotational contact between the drive ring 10 and the internal threaded ring 5, reducing the friction between the drive ring 10 and the internal threaded ring 5, thus making the cleaning of the sight glass tube 3 lens more labor-saving and effectively reducing the energy consumption of the drive motor 6.

[0039] The aquatic biological measuring device can be effectively pulled by attaching it to the connecting ring 16 with external ropes or the like.

[0040] Example 2

[0041] The spectral-based aquatic bioassay device provided in Example 1 has been further optimized. For details, please refer to [link / reference needed]. Figure 3 It also includes an intermediate connector 17, through which the outer shell 1 is detachably connected to the viewing lens barrel 3, and the lens cleaning mechanism is detachably installed at the intermediate connector 17; furthermore, the outer shell 1 and the viewing lens barrel 3 are threaded or socketed to the intermediate connector 17; sealing and waterproof components such as sealing rings can be added at the connection between the intermediate connector 17 and the outer shell 1 and the viewing lens barrel 3.

[0042] The end of the guide rod 13 is equipped with an anti-detachment cap 18.

[0043] The spectral-based aquatic organism detection device provided in this embodiment can replace different photoelectric detection units according to different detection needs. Under the action of the intermediate connector 17, the outer shell 1 and the photoelectric detection unit can be quickly disassembled and assembled. The lens cleaning mechanism can be reused without replacing the entire device, which can effectively save detection costs. The anti-detachment cap 18 can prevent the internal threaded ring 5 from detaching from the end of the guide rod 13.

[0044] The usage process of the spectral-based aquatic biological measurement device provided by this utility model is as follows: The device is directly immersed in water for in-situ detection or installed on a buoy system. One end of the external traction rope is attached to the connecting ring 16, and the other end is attached to the buoy system. The device is then immersed in the water body to be measured. In its natural state, under the action of the spring 12, the transmission rod 11 and the cleaning brush 9 are nearly parallel to the central axis of the sight glass tube 3, and the cleaning brush 9 is detached from the outer wall of the sight glass tube 3. Biological measurements are then performed on the water. When the sight glass lens becomes dirty... When the drive motor 6 is started, the drive motor 6 drives the external threaded cylinder 4 to rotate. After the thread transmission, the internal threaded ring 5 moves along the guide rod 13 towards the lens side of the sight tube 3. When the internal threaded ring 5 pushes the drive ring 10 to move, after the transmission rod 11, the cleaning brush 9 rotates around the hinge point until the cleaning brush 9 is in close contact with the lens on the sight tube 3. At this time, the internal threaded ring 5 is located at the smooth part 8 of the external threaded cylinder 4 and stops moving. The external threaded cylinder 4 continues to rotate, and the cleaning brush 9 cleans the lens of the sight tube 3, cleaning the lens dirt on the sight tube 3 by itself.

[0045] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0046] Obviously, the embodiments described above are only some embodiments of this utility model, not all embodiments. The accompanying drawings show preferred embodiments of this utility model, but do not limit the patent scope of this utility model. This utility model can be implemented in many different forms; rather, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of this utility model. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing specific embodiments, or make equivalent substitutions for some of the technical features. Any equivalent structures made using the content of this utility model specification and drawings, directly or indirectly applied to other related technical fields, are similarly within the patent protection scope of this utility model.

Claims

1. A spectral-based in-water organism measuring device, comprising a housing (1), a signal acquisition circuit board (2) built in the housing (1), and a photoelectric detection unit connected with the housing (1), the photoelectric detection unit being electrically connected with the signal acquisition circuit board (2), the photoelectric detection unit comprising a view mirror tube (3) and a photoelectric detection assembly built in the view mirror tube (3); characterized in that, The lens cleaning mechanism is installed on the view mirror barrel (3), and comprises an outer threaded cylinder (4) rotatably installed on the view mirror barrel (3), an inner threaded ring (5) screwed on the outer threaded cylinder (4), a driving motor (6) fixedly installed outside the view mirror barrel (3), and a lens cleaning piece installed at the end of the view mirror barrel (3), wherein the output end of the driving motor (6) is in transmission connection with the outer threaded cylinder (4), and a threaded portion (7) and a smooth portion (8) are arranged on the outer wall of the outer threaded cylinder (4); The lens cleaning piece comprises two cleaning brushes (9) hingedly installed at the end of the outer threaded cylinder (4), a driving ring (10) radially sliding along the smooth portion (8) of the outer threaded cylinder (4), and two transmission rods (11), one end of each of the two transmission rods (11) is hingedly connected with the end of the cleaning brush (9) away from the center of the outer threaded cylinder (4), and the other end of each of the two transmission rods (11) is hingedly connected with the driving ring (10), a plurality of springs (12) are arranged between the driving ring (10) and the end of the outer threaded cylinder (4) in a circumferential direction, and a guide rod (13) is fixedly installed on the view mirror barrel (3), and the inner threaded ring (5) is slidingly installed on the guide rod (13).

2. The spectroscopic based in-water organism assay device of claim 1, wherein, A tension spring (14) is sleeved on the guide rod (13), one end of the tension spring (14) is fixedly connected with the inner threaded ring (5), and the other end of the tension spring (14) is fixedly connected with the end of the view mirror barrel (3) away from the lens cleaning mechanism.

3. The spectroscopic based in-water bioassay device of claim 2, wherein, A plurality of rolling balls (15) are rotatably installed on the end of the inner threaded ring (5) in contact with the driving ring (10), and the inner threaded ring (5) and the driving ring (10) are in rotatable contact through the rolling balls (15).

4. The spectroscopic based in-water bioassay device of claim 1, wherein, A connecting lifting ring (16) is installed on the shell (1).

5. The spectroscopic based in-water bioassay device of claim 4, wherein, An intermediate connecting body (17) is further arranged, the shell (1) is detachably connected with the view mirror barrel (3) through the intermediate connecting body (17), and the lens cleaning mechanism is installed on the intermediate connecting body (2).

6. The spectroscopic based in-water bioassay device of claim 1, wherein, A anti-disengagement end cover (18) is installed on the end of the guide rod (13).

7. The spectroscopic based in-water biological assay device of claim 1, wherein, The photoelectric detection assembly at least comprises a light source (19), a light guide column (20), a receiving filter (21), and a detector (22). The photoelectric detection assembly at least comprises a light source (19), a light guide column (20), a receiving filter (21), and a detector (22).

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