Nucleic acid amplification tube
By designing storage and drainage mechanisms in the nucleic acid amplification tube, the problem of insufficient self-contained reagents is solved, enabling convenient mixing of reagents and samples and contamination-free pouring, thus ensuring the accuracy of test results.
Patent Information
- Application Number
- CN202520303914.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-25
- Publication Date
- 2026-01-16
- Estimated Expiration
- 2035-02-25
AI Technical Summary
Existing nucleic acid amplification tubes do not come with their own reagents, which makes them prone to contamination during mixing, and the sample pouring process is complicated, affecting the accuracy of the test.
A nucleic acid amplification tube was designed, which has a storage mechanism and a discharge mechanism. The storage mechanism is filled with reagents and uses solid paraffin, and the discharge mechanism achieves uncontaminated sample discharge through a circular sleeve and a conical structure.
It enables convenient mixing of reagents and samples, avoids sample contamination, and ensures the accuracy of test results.
Smart Images
Figure CN223805087U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to nucleic acid detection technical field, concretely is a nucleic acid amplification tube. BACKGROUND
[0002] There are various techniques to realize pathogen detection method, wherein the most common method includes two categories, one is to adopt immune method to directly detect antigen protein or detect antibody (IgG or IgM) secreted by organism to specific antigen of invading pathogen, the other is to determine whether the microorganism to be detected exists by detecting pathogen nucleic acid, wherein nucleic acid can be rapidly obtained by various amplification techniques in vitro to obtain specific section amplification product of several million to several hundred million times, and nucleic acid is the main basis for species distinction as the genetic material of organism, therefore nucleic acid amplification detection technology, namely molecular diagnosis technology in popular sense, obtains more and more application in clinical examination and life science field, nucleic acid in vitro amplification technology mainly has traditional variable temperature PCR (polymerase chain reaction) technology and isothermal amplification technology, such as LAMP (loop-mediated isothermal amplification technology), TMA (transcription-mediated amplification technology), RPA (recombinase polymerase amplification technology) and the like.
[0003] In the prior art, the conventional nucleic acid amplification tube does not have self-contained reagent, so that multiple test tubes need to be poured to each other to mix the reagent and the medicine, which is prone to cause pollution, resulting in inaccurate detection result, and the sample needs to be poured by opening the sealing cover, which is also prone to cause sample pollution, and the use is very inconvenient. SUMMARY
[0004] The utility model provides a nucleic acid amplification tube to solve the problem in the prior art.
[0005] To achieve the above object, the utility model provides the following technical scheme: a nucleic acid amplification tube, including the pipe body, the upper and lower sides of the pipe body are open structure, the upper surface of the pipe body is equipped with the sealing cover, the lower surface of the sealing cover is provided with storage mechanism, the lower surface of the pipe body is fixedly installed with the sealing membrane, the sealing membrane corresponding pipe body lower surface is equipped with the drainage mechanism.
[0006] Further, the storage mechanism includes a storage cavity, a reagent and solid paraffin, the lower surface of the sealing cover is provided with a storage cavity, the storage cavity is filled with a reagent, and the inner wall of the storage cavity under the reagent is sealedly installed with solid paraffin.
[0007] Further, the lower surface of the solid paraffin is horizontally flush with the lower surface of the sealing cover, and the melting point of the solid paraffin is 35-52 DEG C.
[0008] Further, the liquid discharge mechanism comprises a sleeve, a partition plate, a cone and a liquid discharge channel, the sleeve is threadedly installed on the outer surface of the lower end of the tube body, the partition plate is fixedly installed on the inner wall of the sleeve close to the lower side, the upper surface of the partition plate is attached to the lower surface of the sealing film, the cone is fixedly embedded on the partition plate, and the liquid discharge channel is formed on the cone close to the front and rear positions.
[0009] Further, the lower side of the cone is a pointed structure, and the lower surface of the cone is 2mm-4mm away from the lower surface of the sleeve.
[0010] Further, the front side of the tube body is provided with scale lines.
[0011] Compared with the prior art, the nucleic acid amplification tube has the following beneficial effects:
[0012] The nucleic acid amplification tube has the functions of increasing the reagent self-provided function of the nucleic acid amplification tube through the storage mechanism, facilitating the mixing of the reagent and the sample, effectively avoiding the problem that the sample is contaminated, the liquid discharge mechanism facilitates the pouring of the sample, the sample is not easy to be contaminated, and the detection structure is ensured to be accurate. BRIEF DESCRIPTION OF DRAWINGS
[0013] Fig. 1 It is a structural schematic view of the utility model;
[0014] Fig. 2 It is a tube body sectional view of the utility model;
[0015] Fig. 3 It is a left view sectional view of the cone of the utility model.
[0016] In the drawing: 1, tube body; 2, sealing cover; 3, storage mechanism; 301, storage cavity; 302, reagent; 303, solid paraffin; 4, sealing film; 5, liquid discharge mechanism; 501, sleeve; 502, partition plate; 503, cone; 504, liquid discharge channel; 6, scale line. DETAILED DESCRIPTION
[0017] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.
[0018] Please refer to Figs. 1-3The utility model discloses a nucleic acid amplification tube, including the tubulature 1, the upside and downside of tubulature 1 are open structure, the upper surface of tubulature 1 is equipped with the sealing cover 2 of screw thread, the lower surface of sealing cover 2 is provided with storage mechanism 3, the lower surface fixed mounting of tubulature 1 is equipped with sealing membrane 4, sealing membrane 4 corresponding tubulature 1 lower surface screw thread is equipped with drainage mechanism 5.
[0019] Specific, the storage mechanism 3 includes storage cavity 301, reagent 302 and solid paraffin 303, the lower surface of sealing cover 2 is provided with storage cavity 301, the storage cavity 301 is filled with reagent 302, and the inner wall of the storage cavity 301 of the lower surface of the reagent 302 is sealedly installed with solid paraffin 303.
[0020] In the embodiment, the solid paraffin 303 is heated and melted by the constant-temperature bath, so that the reagent 302 flows from the storage cavity 301 into the tubulature 1, the reagent 302 is mixed with the sample, and the mixture is shaken in the constant-temperature bath.
[0021] Specific, the lower surface of the solid paraffin 303 is horizontally flush with the lower surface of the sealing cover 2, and the melting point of the solid paraffin 303 is 35-52 DEG C.
[0022] In the embodiment, the lower surface of the solid paraffin 303 is flush with the sealing cover 2, and the solid paraffin 303 is easy to melt.
[0023] Specific, the drainage mechanism 5 includes a circular sleeve 501, a baffle 502, a cone 503 and a drainage passage 504, the lower end surface of the tubulature 1 is screw-threadedly installed on the outer surface of the circular sleeve 501, the baffle 502 is fixedly installed on the inner wall of the circular sleeve 501 close to the lower side, the upper surface of the baffle 502 is attached to the lower surface of the sealing membrane 4, the baffle 502 is fixedly embedded with the cone 503, and the drainage passage 504 is formed in the cone 503 close to the front and rear positions.
[0024] In the embodiment, when the baffle 502 on the circular sleeve 501 is attached to the sealing membrane 4, the sealing membrane 4 is protected, the circular sleeve 501 is rotated by 180 degrees, the sharp head of the cone 503 is upward, the circular sleeve 501 is moved upward along the tubulature 1, the baffle 502 drives the cone 503 to move upward, and the sample after the mixed reagent 302 flows out through the multiple drainage passages 504 in the cone 503.
[0025] Specific, the lower side of the cone 503 is a sharp head structure, and the lower surface of the cone 503 is 2mm-4mm away from the lower surface of the circular sleeve 501.
[0026] In the embodiment, the cone 503 does not hinder the placement of the circular sleeve 501.
[0027] Specifically, the front side of the pipe body 1 is provided with a scale line 6.
[0028] In the embodiment, the dose of the sample added by the scale line 6 can be viewed.
[0029] In use, the pipe body 1 can be initially filled with A reagent, the sealing cover 2 is opened, the sample is easily poured into the pipe body 1, the dose of the sample added is viewed through the scale line 6, the sealing cover 2 is screwed on the upper surface of the pipe body 1 to seal the pipe body 1, and the pipe body 1 is placed in a constant temperature bath to heat and shake the pipe body 1. At this time, the solid paraffin 303 is melted by the heating of the constant temperature bath, the reagent 302 flows into the pipe body 1 from the storage cavity 301, the reagent 302 is mixed with the sample, and the mixture is shaken in the constant temperature bath. The temperature of the constant temperature metal bath is set to 60 DEG C, and after standing for 5 minutes, the reagent 302 is fully mixed and transferred to a constant temperature amplifier for reaction at 56 DEG C for 15 minutes. The constant temperature amplifier is a GS8 model produced by the First Gene Technology Co., Ltd., the Fam fluorescence channel is scanned once every 30 seconds, the result is automatically interpreted according to the software, and the positive and negative results after detection are compared with the results of the fluorescent quantitative PCR diagnosis. If the detection test paper is used for detection, the circular sleeve 501 in the liquid discharge mechanism 5 is rotated by 180 degrees, the sharp tip of the conical body 503 is upward at this time, the circular sleeve 501 moves upward along the pipe body 1, the circular sleeve 501 drives the conical body 503 to move upward through the partition plate 502, the plurality of liquid discharge channels 504 in the conical body 503 make the sample mixed with the reagent 302 flow to the detection test paper, the function of the nucleic acid amplification tube with the reagent 302 is increased, the reagent 302 is easily mixed with the sample, the problem of sample contamination is effectively avoided, the sample is easily poured out, the sample is not easily contaminated, and the detection structure is accurate.
[0030] In summary, the nucleic acid amplification tube increases the function of the nucleic acid amplification tube with the reagent 302, the reagent 302 is easily mixed with the sample, the problem of sample contamination is effectively avoided, the sample is easily poured out, the sample is not easily contaminated, and the detection structure is accurate.
[0031] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.
Claims
1. A nucleic acid amplification tube comprising a tube body (1), characterized in that: The upper and lower sides of the pipe body (1) are open structures, the upper surface of the pipe body (1) is provided with a sealing cover (2) in a threaded manner, the lower surface of the sealing cover (2) is provided with a storage mechanism (3), the lower surface of the pipe body (1) is fixedly provided with a sealing film (4), and the lower surface of the sealing film (4) is provided with a drainage mechanism (5) in a threaded manner.
2. The nucleic acid amplification tube of claim 1, wherein: The storage mechanism (3) comprises a storage cavity (301), a reagent (302) and solid paraffin (303), the lower surface of the sealing cover (2) is provided with a storage cavity (301), the storage cavity (301) is filled with a reagent (302), and the inner wall of the storage cavity (301) on the lower surface of the reagent (302) is sealingly provided with solid paraffin (303).
3. A nucleic acid amplification tube according to claim 2, wherein: The lower surface of the solid paraffin (303) is horizontally flush with the lower surface of the sealing cover (2), and the melting point of the solid paraffin (303) is 35-52℃.
4. The nucleic acid amplification tube of claim 1, wherein: The drainage mechanism (5) comprises a circular sleeve (501), a partition (502), a cone (503) and a drainage channel (504), the lower end surface of the pipe body (1) is provided with a circular sleeve (501) in a threaded manner, the inner wall of the circular sleeve (501) is fixedly provided with a partition (502) close to the lower side, the upper surface of the partition (502) is attached to the lower surface of the sealing film (4), the partition (502) is fixedly embedded with a cone (503), and the cone (503) is provided with a drainage channel (504) close to the front and rear positions.
5. The nucleic acid amplification tube of claim 4, wherein: The lower side of the cone (503) is a sharp structure, and the lower surface of the cone (503) is 2mm-4mm away from the lower surface of the circular sleeve (501).
6. The nucleic acid amplification tube of claim 1, wherein: The front side of the pipe body (1) is provided with a scale line (6).