Automatic preparation device for stem cell supernate

Abstract

The utility model discloses an automatic preparation device for stem cell supernate, which relates to the technical field of automatic preparation of biological products, and is characterized in that a pressure plate is driven by a pneumatic cylinder to extrude and preliminarily cut tissues, a rotating shaft is driven by a motor to rotate to drive a cutting knife to perform secondary cutting to ensure that tissue fragments are uniform and fine, and a filter box is provided with a plurality of filter holes. Undigested tissue fragments are effectively intercepted, the separation barrel further improves the liquid separation efficiency, the water bath box provides a constant temperature environment, the stability of the enzyme digestion process is guaranteed, the stirring rod prevents tissue precipitation, and the cutting and filtering efficiency is improved; efficient discharge and introduction of liquid are achieved through the pairing design of the separation ring and the quick connector, the leakage risk is reduced, cross contamination is prevented, the cutting effect and liquid flowing are enhanced through ultrasonic vibration applied by the piezoelectric stack, and the overall treatment efficiency is further improved; through a highly automatic operation process, the preparation efficiency and quality of the stem cell supernatant are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the automation preparation technical field of biological products, and specifically relates to a stem cell supernatant automation preparation device. BACKGROUND

[0002] With the rapid development of stem cell research and clinical application, the purity and preparation efficiency of stem cell supernatant, as an important product in the stem cell culture process, directly affect the subsequent scientific research and clinical effect. In the prior art, the extraction and collection of stem cell supernatant mainly rely on physical methods such as centrifugation and filtration. However, these methods still have certain limitations in terms of operation complexity, automation degree and processing efficiency.

[0003] Chinese utility model patent CN219950972U discloses a placenta stem cell supernatant extraction and collection device. The device includes an outer cylinder and an extraction mechanism. The extraction mechanism realizes precise extraction of supernatant in the culture solution through the cooperation of a motor, a main gear, a slave gear and a transmission rod. The device separates by forming centrifugal force through rotation, which can improve the extraction effect and efficiency, and the operation is relatively simple. However, the design of the device in terms of automation control, cleaning function and temperature control is relatively basic, and it cannot realize high automation and intelligent control in the whole process. Moreover, there may be problems of insufficient efficiency when processing complex tissue samples.

[0004] Chinese invention patent CN114085769B discloses a centrifugal intelligent extraction system for stem cell extraction. The system includes a mounting base plate, a support column, a centrifugal shell, a collection box and a liquid outlet pipe. The system drives stirring fan blades through multiple shafts and gears to stir placenta fragments and collagenase solution, promoting digestion reaction. The system accelerates the digestion reaction speed and improves the extraction efficiency by combining stirring and centrifugation. However, the design of the system in terms of liquid filtration, ultrasonic assisted cutting, automatic cleaning and temperature control is not perfect, and it is difficult to realize stable preparation of high-purity supernatant. At the same time, there is still room for improvement in terms of automation degree and operation convenience. SUMMARY

[0005] In view of the above deficiencies in the prior art, the utility model provides a stem cell supernatant automation preparation device. By integrating multiple key steps such as cutting, filtering, separating, cleaning, enzyme digestion and centrifugation, the device realizes high automation operation in the whole process. The device introduces a stirring rod to prevent tissue sedimentation, a quick connector to realize efficient liquid processing and connection, a piezoelectric stack to apply ultrasonic vibration to enhance cutting effect, and automatic cleaning and liquid injection functions. The purity and preparation efficiency of the supernatant are greatly improved, and the problems of low automation degree, complex operation and insufficient processing efficiency in the prior art are solved, which has significant technical progress and broad application prospects.

[0006] The purpose of this utility model is achieved through the following technical solution: an automated stem cell supernatant preparation device, comprising a cutting cylinder, a cutting plate slidably connected inside the cutting cylinder along its axial direction, a rotating shaft rotatably connected to the bottom end of the cutting plate, a cutting blade fixedly connected to the outer end of the rotating shaft near its top end, a filter box slidably connected to the outer end of the rotating shaft, a plurality of filter holes being opened at the bottom end of the filter box, a separation cylinder slidably connected to the outer end of the filter box, the separation cylinder being slidably connected to the outer end of the rotating shaft along its axial direction, a water bath box being provided outside the filter box, the rotating shaft penetrating downward through the water bath box, and a motor slidably connected to the penetrating part along its axial direction, a piezoelectric stack being fixedly connected to the bottom end of the rotating shaft through the motor, a pressure plate slidably connected inside the cutting cylinder at the top end of the cutting plate, and a bracket fixing the bottom end of the cutting cylinder and the top end of the filter box.

[0007] A pneumatic cylinder is fixedly connected to the top of the pressure plate. The cylinder body is fixedly connected to the outer end of the cutting cylinder through a bracket. The piston rod of the pneumatic cylinder is fixedly connected to the top of the pressure plate. The cutting plate is a grid structure composed of multiple blades. The motor rotor is slidably connected to the rotating shaft through a keyway.

[0008] A uniform distribution disk is fixedly connected to the outer end of the rotating shaft at the bottom of the cutting blade. The uniform distribution disk has a conical structure, and the tip of the conical structure is set close to the cutting blade. The top of the cutting blade is in contact with the bottom of the cutting plate.

[0009] The outer diameter of the distribution disc is equal to the inner diameter of the top of the separation cylinder. A sealing ring is provided between the cutting cylinder and the separation cylinder. The top of the sealing ring is fixedly connected to the bottom of the cutting cylinder, and the bottom of the sealing ring is rotatably connected to the top of the separation cylinder. The sealing ring is made of flexible material.

[0010] A stirring rod is fixedly connected to the outer end of the rotating shaft at a position between the distribution plate and the filter box, with the stirring rod being two to three centimeters away from the top of the inner wall of the filter box.

[0011] A separation ring is fixedly connected to the outer end of the separator near its bottom. The separation ring is connected to the inner wall of the separator, and multiple quick-connect couplings are fixedly connected to the bottom of the separation ring.

[0012] The water bath tank has a corresponding lower quick connector fixedly connected to the upper quick connector at the same position. The bottom of the lower quick connector is connected to the external equipment through a pipe. Each lower quick connector is paired with the corresponding upper quick connector.

[0013] A spring is fixedly connected to the bottom of the piezoelectric stack, and a base is fixedly connected to the bottom of the spring. The cutting cylinder, water bath, and motor are all fixedly connected to the base. Multiple unidirectional nozzles are fixedly connected to the pressure plate, and all of the nozzles are connected to an external system.

[0014] The beneficial effects of this utility model are:

[0015] 1. The device integrates multiple key steps such as tissue cutting, filtering, separation, washing, enzyme digestion, culture, and centrifugation, realizes automatic operation of the whole process, greatly reduces manual intervention, improves work efficiency and operation consistency.

[0016] 2. The cutting plate with multi-blade grid structure and the rotating cutting knife can perform primary and secondary cutting on the tissue, ensuring that the tissue fragments are uniform and small, and improving the purity of the supernatant.

[0017] 3. The filter box with multiple filter holes can effectively intercept undigested tissue fragments, ensuring the purity of the supernatant, and the combination of the separation cylinder and the uniform distribution disc further improves the separation efficiency of the liquid, ensuring the quality of the supernatant.

[0018] 4. The water bath box provides a constant temperature control environment, ensuring that the enzyme digestion process is carried out at an appropriate temperature, which helps to maintain the activity and stability of cells and tissues, and improves the digestion effect.

[0019] 5. The introduction of the stirring rod effectively prevents the precipitation and aggregation of the tissue in the filter box, ensuring uniform contact between the liquid and the tissue, improving the cutting and filtering efficiency, and promoting the full action of the enzyme.

[0020] 6. The pair design of the quick connector realizes the rapid discharge and introduction of the liquid, simplifies the liquid processing process, reduces the risk of leakage, and ensures the efficient flow and safety of the liquid between different processing links.

[0021] 7. The design of the spring and the base effectively buffers and absorbs the vibration during the operation of the device, protects the internal structure, prolongs the service life of the device, and ensures long-term stable operation.

[0022] 8. The one-way spray head realizes automatic cleaning and automatic injection of the culture medium in the device, reduces manual operation, improves work efficiency and operation consistency, and reduces the risk of human operation errors.

[0023] 9. The ultrasonic vibration applied by the piezoelectric stack enhances the cutting ability of the cutting plate and the cutting knife, while promoting the flow of the liquid and the filtration process, improving the overall processing efficiency and effect, ensuring the efficiency and accuracy of the cutting and separation process, realizing the high automation of the preparation process of the stem cell supernatant, significantly improving the work efficiency and product consistency, and meeting the demand for high-purity supernatant in scientific research and clinical application. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is the overall structure diagram of the utility model;

[0025] Figure 2 It is the overall explosion diagram of the utility model;

[0026] Figure 3 For the localized explosion of this utility model Figure 1 ;

[0027] Figure 4 For the localized explosion of this utility model Figure 2 ;

[0028] Figure 5 This is a front view of the present invention;

[0029] Figure 6 For the present utility model Figure 5 Sectional view of AA;

[0030] Figure 7 This is a structural diagram of the present utility model.

[0031] Explanation of the labels in the diagram

[0032] 1. Cutting cylinder; 2. Cutting plate; 3. Rotating shaft; 4. Cutting blade; 5. Filter box; 6. Separation cylinder; 7. Water bath; 8. Motor; 9. Piezoelectric stack; 10. Pressure plate; 11. Pneumatic cylinder; 12. Distribution plate; 13. Sealing ring; 14. Stirring rod; 15. Separation ring; 16. Upper quick connector; 17. Lower quick connector; 18. Spring; 19. Base. Detailed Implementation

[0033] The technical solution of this utility model will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the protection scope of this utility model.

[0034] It should be noted that the directional concepts of "left", "right", "up", "down", "front", "back", "inner", and "outer" in the following scheme are all relative directions, and will not be listed one by one here.

[0035] Example 1:

[0036] like Figures 1 to 7 As shown in the figure, this embodiment describes in detail the basic structure and core functions of an automated stem cell supernatant preparation device, which mainly includes the following components:

[0037] The cutting cylinder 1 serves as the main structure of the entire device, providing the primary space for tissue cutting and processing.

[0038] The cutting plate 2 is slidably connected to the inside of the cutting cylinder 1 along the axial direction of the cutting cylinder 1, and is used to perform preliminary cutting on the injected tissue.

[0039] The rotating shaft 3 is fixedly connected to the bottom end of the cutting plate 2 and rotates along the axis direction of the cutting cylinder 1.

[0040] The cutting knife 4 is fixedly connected to the outer end of the rotating shaft 3 close to the top end, is located at the bottom end of the cutting plate 2 and is in contact with the cutting plate 2, and is responsible for secondary cutting of the cut tissues.

[0041] The filter box 5 is slidingly connected to the cutting cylinder 1 through the outer end of the rotating shaft 3 and is fixedly connected to the bottom end of the cutting cylinder 1 through a support, and the bottom end of the filter box 5 is provided with a plurality of filter holes for filtering the cut tissue fragments.

[0042] The separation cylinder 6 is slidingly connected to the outer end of the rotating shaft 3 along the axis direction thereof and is located at the outer end of the filter box 5 and is used for further separating and processing the filtered liquid.

[0043] The water bath box 7 is arranged outside the filter box 5, the rotating shaft 3 penetrates downward through the water bath box 7, and a temperature control environment is provided.

[0044] The motor 8 is slidingly connected to the water bath box 7 along the axis direction of the rotating shaft 3 and drives the rotation of the rotating shaft 3.

[0045] The piezoelectric stack 9 is fixedly connected to the penetrating part of the bottom end of the rotating shaft 3 and is driven by the motor 8 to apply ultrasonic vibration to assist the cutting and separation process.

[0046] In addition, the pressing plate 10 is slidingly connected to the position of the cutting plate 2 at the top end in the cutting cylinder 1, the input and extrusion of the tissues can be controlled through the pressing plate 10, the cutting cylinder 1 is fixedly connected to the top end of the filter box 5 through a support, and the stability of the overall structure is ensured.

[0047] Working process

[0048] Tissue input and preliminary cutting:

[0049] The operator lifts up the pressing plate 10 to input the tissues to be cut into the top end of the cutting cylinder 1.

[0050] The pressing plate 10 is pushed downward by the pneumatic cylinder 11 to extrude the tissues, so that the tissues are preliminarily cut through the cutting plate 2 under the action of pressure.

[0051] Secondary cutting and distribution:

[0052] The motor 8 drives the rotating shaft 3 to rotate, the rotating shaft 3 in turn drives the cutting knife 4 to rotate, the tissues cut through the cutting plate 2 are secondarily cut to ensure the uniformity and smallness of the tissue fragments.

[0053] The cut tissues slide along the outer end of the uniform distribution disc 12 to the top end of the separation cylinder 6 under the action of gravity, and the uniform distribution disc 12 uniformly distributes the tissue fragments into the filter box 5.

[0054] Filtering and preliminary separation:

[0055] The twice-cut tissue enters the filter box 5 from the uniform distribution disc 12 and the separation cylinder 6, and the multiple filter holes at the bottom of the filter box 5 filter the undigested tissue fragments to ensure the purity of the supernatant.

[0056] Temperature regulation and ultrasonic assistance:

[0057] After cleaning, the required enzyme is added to the filter box 5, and the enzyme is used to digest the tissue fragments.

[0058] The rotating shaft 3 is provided with temperature regulation through part of the water bath box 7, maintaining the appropriate environmental temperature to ensure the stability of the tissue processing process.

[0059] The piezoelectric stack 9 continuously applies ultrasonic vibration to enhance the cutting efficiency of the cutting plate 2 and the cutting knife 4, and to promote the flow of liquid in the filtering and separation process, improving the overall processing effect.

[0060] Through the cooperation of the air cylinder 11 and the motor 8, automation operation of multiple links such as tissue input, cutting, and filtering is realized, reducing manual intervention and improving work efficiency and operation consistency.

[0061] The cutting plate 2 is composed of a grid structure of multiple blades, which cooperates with the secondary cutting design of the cutting knife 4 to effectively cut the tissue into uniform and small fragments, ensuring the purity of the supernatant.

[0062] The multiple filter holes (100-200 mesh filter) at the bottom of the filter box 5 can effectively filter out undigested tissue fragments, and the design of the separation cylinder 6 further improves the separation efficiency of the liquid, ensuring the quality of the supernatant.

[0063] The water bath box 7 provides a stable temperature regulation environment, ensuring that the cutting and separation process is carried out at an appropriate temperature, which is conducive to maintaining the activity and stability of cells and tissues.

[0064] The ultrasonic vibration applied by the piezoelectric stack 9 enhances the cutting ability of the cutting plate 2 and the cutting knife 4, while promoting the flow of liquid and the filtering process, improving the overall processing efficiency and effect.

[0065] Each component is stably connected through a bracket and sliding connection, with a reasonable structure design, facilitating daily maintenance and cleaning, and prolonging the service life of the equipment.

[0066] Through the above design and function, the embodiment provides a high-efficiency, stable, and highly automated stem cell supernatant preparation device, which can meet the demand for high-purity supernatant in scientific research and clinical application, and improve the overall efficiency and quality of stem cell culture and processing.

[0067] Example 2

[0068] As Figures 1 to 7 shown, on the basis of embodiment 1, this embodiment further improves the automatic preparation device of stem cell supernatant, adds stirring function, quick connector connection mode, structure stability enhancement and automatic cleaning system, and the specific implementation is as follows:

[0069] The stirring rod 14 is fixedly connected to the outer end of the rotating shaft 3 and located between the uniform distribution disc 12 and the filter box 5.

[0070] The stirring rod 14 is kept at a distance of two to three centimeters from the top end of the inner wall of the filter box 5, which ensures effective stirring during liquid flow and prevents sedimentation and aggregation.

[0071] The separation ring 15 is fixedly connected to the position close to the bottom end of the separation cylinder 6.

[0072] The separation ring 15 is connected to the inner wall of the separation cylinder 6, ensuring smooth flow of the liquid.

[0073] The bottom end of the separation ring 15 is fixedly connected to a plurality of upper quick connectors 16, which are used for paired connection with the lower quick connectors 17 to realize quick discharge and introduction of the liquid.

[0074] The water bath box 7 is provided with a lower quick connector 17 corresponding to each upper quick connector 16.

[0075] Each lower quick connector 17 is connected to an external device through a pipeline to realize efficient connection with an external system.

[0076] Each lower quick connector 17 is paired with the corresponding upper quick connector 16 to ensure the sealing and safety of the liquid during discharge and introduction.

[0077] The bottom end of the piezoelectric stack 9 is fixedly connected to a spring 18 for buffering and absorbing vibration to protect the internal structure of the device.

[0078] The bottom end of the spring 18 is fixedly connected to a base 19, which serves as the basic support structure of the entire device.

[0079] The cutting cylinder 1, the water bath box 7 and the motor 8 are all fixedly connected through the base 19 to ensure the structural stability of the device during operation and reduce the loosening or displacement of parts caused by vibration.

[0080] A plurality of one-way nozzles 20 are fixedly connected to the pressing plate 10, and the nozzles 20 are connected to an external system.

[0081] The one-way nozzles 20 are used to spray cleaning liquid and culture liquid into the cutting cylinder 1 during cleaning and culture to realize automatic liquid treatment and device cleaning.

[0082] Working process

[0083] The operator lifts the pressing plate 10 upward and puts the tissue to be cut into the top end of the cutting cylinder 1.

[0084] The pressing plate 10 is pushed down by the air cylinder 11, extruding the tissue and making it preliminarily cut by the cutting plate 2 under pressure.

[0085] The motor 8 drives the rotation of the rotating shaft 3, which in turn drives the rotation of the cutting knife 4, for secondary cutting of the tissue cut by the cutting plate 2, ensuring uniform and fine tissue fragments.

[0086] The stirring rod 14 continuously rotates under the drive of the rotating shaft 3, uniformly stirring the liquid and tissue in the filter box 5, preventing tissue sedimentation and improving cutting and filtering efficiency.

[0087] The cut tissue slides down along the outer end of the uniform distribution disc 12 to the top end of the separation cylinder 6 under the action of gravity.

[0088] The twice-cut tissue enters the filter box 5 between the uniform distribution disc 12 and the separation cylinder 6, and the multiple filter holes at the bottom end of the filter box 5 filter the undigested tissue fragments, ensuring the purity of the supernatant.

[0089] Through the one-way spray head 20 on the pressing plate 10, clean water is sprayed into the cutting cylinder 1, thoroughly cleaning the cutting plate 2, cutting knife 4, uniform distribution disc 12 and tissue, ensuring that there is no residue inside the equipment.

[0090] During the cleaning process, the stirring rod 14 continuously rotates to uniformly stir the tissue, improving the cleaning effect.

[0091] After cleaning, the cleaned liquid is quickly discharged through the quick couplings 16 and 17, ensuring timely replacement of the liquid inside the equipment and efficient connection of external equipment.

[0092] After cleaning, the required enzyme is added to the filter box 5 for digestion of the tissue fragments.

[0093] The paired arrangement of the quick couplings 16 and 17 ensures the sealing and safety of the liquid during discharge and introduction, preventing cross-contamination.

[0094] The piezoelectric stack 9 is connected to the base 19 by the spring 18, which buffers and absorbs vibrations during equipment operation, protecting the internal structure and prolonging the service life of the equipment.

[0095] The base 19 serves as a foundation support, ensuring the stable connection of the cutting cylinder 1, water bath box 7 and motor 8, reducing the loosening or displacement of components caused by vibration.

[0096] The connection of the one-way spray head 20 with the external system realizes the automatic spraying and replacement of the cleaning liquid and the culture liquid, reduces manual operation, improves work efficiency and operation consistency.

[0097] The introduction of the stirring rod 14 effectively prevents the sedimentation and aggregation of tissues in the filter box 5, ensures uniform contact between the liquid and the tissues, and improves the efficiency of cutting and filtering.

[0098] The quick connection of the separation ring 15 with the upper quick connector 16 and the lower quick connector 17 realizes efficient discharge and introduction of the liquid, simplifies the operation process, and reduces the risk of leakage during liquid processing.

[0099] The design of the spring 18 and the base 19 effectively buffers the ultrasonic vibration applied by the piezoelectric stack 9, protects the internal structure of the device, prolongs the service life of the device, and ensures long-term stable operation.

[0100] The connection of the one-way spray head 20 with the external system realizes the automatic cleaning of the device and the automatic spraying of the culture liquid, reduces manual intervention, improves work efficiency and operation consistency, and reduces the risk of human operation errors.

[0101] The paired design of the quick connectors 16 and 17 ensures the sealing of the liquid during discharge and introduction, prevents cross-contamination between different liquids, and guarantees the purity of the supernatant and the safety of cell culture.

[0102] Each component is fixedly connected through the base 19 and the bracket, the structure is reasonably designed, daily maintenance and cleaning are convenient, the maintenance cost and time of the device are reduced, and the use convenience is improved.

[0103] Through the cooperative work of the air cylinder 11, the piezoelectric stack 9, the stirring rod 14, the quick connectors 16 and 17, and the one-way spray head 20, the preparation process of the stem cell supernatant is highly automated, the work efficiency and product consistency are significantly improved, and the demand for high-purity supernatant in scientific research and clinical application is met.

[0104] When in use, the pressure plate 10 is lifted upward by the pneumatic cylinder 11, and the tissue to be cut is put into the top end of the pressure plate 10, then the pressure plate 10 is pushed downward by the pneumatic cylinder 11, and the tissue is cut by the cutting plate 2 under the pressure, at the same time, the rotating shaft 3 is driven to rotate by the motor 8, and the cutting knife 4, the uniform distribution disc 12, the stirring rod 14, the separation cylinder 6 and the separation ring 15 are driven to rotate by the rotating shaft 3, and the tissue cut by the cutting plate 2 is cut again when the cutting knife 4 rotates, then the tissue slides along the outer end of the uniform distribution disc 12 to the top end of the separation cylinder 6 under the action of gravity, then the twice-cut tissue enters the filter box 5 between the uniform distribution disc 12 and the separation cylinder 6, then the cutting cylinder 1 is sprayed with cleaning liquid by the multiple nozzles on the pressure plate 10 to clean the cutting plate 2, the cutting knife 4, the uniform distribution disc 12 and the tissue, and after cleaning, the separation cylinder 6 is manually pushed downward to make the upper quick connector 16 on the separation ring 15 contact with the corresponding lower quick connector 17 to discharge the cleaned liquid, and the stirring rod 14 continuously rotates to stir the tissue during the cleaning process.

[0105] After cleaning, the required enzyme is added into the filter box 5 by the multiple nozzles, then the pressure plate 10 is moved downward by the pneumatic cylinder 11 to push the cutting plate 2 and the rotating shaft 3 to move downward until the outer end of the uniform distribution disc 12 contacts with the top end of the separation cylinder 6, the filter box 5 is sealed by the uniform distribution disc 12 and the separation cylinder 6, and the water in the water bath box 7 is used to regulate the temperature of the tissue in the filter box 5, and the stirring rod 14 continuously stirs the tissue during the enzyme digestion process.

[0106] After the enzyme digestion is completed, the pressure plate 10, the rotating shaft 3 and the separation cylinder 6 are moved downward by the pneumatic cylinder 11, at this time, the liquid in the filter box 5 flows into the separation ring 15, the filter holes on the filter box 5 intercept the tissue that has not completed digestion, the filter holes at the bottom end of the filter box 5 are 100-200 mesh filter screens, which can automatically filter out the undigested tissue fragments after the digestion is completed, then the digested liquid is taken out by the upper quick connector 16 and the lower quick connector 17, then the device is cleaned by the nozzles on the pressure plate 10, and then the previously taken out digested liquid is put into the filter box 5 again from the top end of the cutting cylinder 1 for culture and subculture, and the culture liquid is replaced regularly by the pressure plate 10 and the upper quick connector 16 to maintain the healthy state of the cells during the culture process.

[0107] After the culture is completed, the subcultured umbilical cord mesenchymal stem cell supernatant enters the separation ring 15, and the separation ring 15 is driven to rotate by the motor 8, and high-speed centrifugal separation is performed during high-speed rotation, and after the centrifugal separation is completed, the supernatant is discharged by the upper quick connector 16 and the lower quick connector 17.

[0108] During the cutting, cleaning, enzyme digestion and culture process, the piezoelectric stack 9 continuously applies ultrasonic vibration to the cutting plate 2, the rotating shaft 3, the uniform distribution disc 12 and the stirring rod 14, and the cutting plate 2 can be better cut through the ultrasonic vibration, and the piezoelectric stack 9 stops vibrating during the separation process.

[0109] Through the above design and function enhancement, embodiment 2 further improves the overall performance and operation convenience of the stem cell supernatant automatic preparation device on the basis of embodiment 1, ensures the efficiency, stability and safety of the preparation process, and meets the high-standard scientific research and clinical needs.

[0110] The above only describes the preferred embodiments of the present application, and it should be understood that the present application is not limited to the form disclosed herein, and should not be considered as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the concept described herein by the above teaching or related technical or knowledge, and the modification and change made by the person skilled in the art without departing from the spirit and scope of the present application should be within the protection scope of the claims attached to the present application.

Claims

1. An automated stem cell supernatant preparation device, characterized by, Including cutting cylinder (1), cutting plate (2) is connected along its axial direction inside the cutting cylinder (1) slidingly, the bottom end of cutting plate (2) is rotatably connected with the rotating shaft (3), the outer end of rotating shaft (3) is fixedly connected with cutting knife (4) near the top end position thereof, the outer end of rotating shaft (3) is slidingly connected with filter box (5), a plurality of filter holes are formed in the bottom end of filter box (5), the outer end of filter box (5) is slidingly connected with separation cylinder (6), the outer end of separation cylinder (6) is slidingly connected with rotating shaft (3) along its axial direction, water bath tank (7) is arranged on the outer side of filter box (5), rotating shaft (3) penetrates water bath tank (7) downward, and the penetrating part is slidingly connected with motor (8) along its axial direction, the bottom end of rotating shaft (3) penetrates motor (8) and the penetrating part is fixedly connected with piezoelectric stack (9), the top end of cutting plate (2) is slidingly connected with pressing plate (10) in the cutting cylinder (1), and the bottom end of cutting cylinder (1) and the top end of filter box (5) are fixedly connected through a support.

2. The stem cell supernatant automated preparation device according to claim 1, wherein: The top end of pressing plate (10) is fixedly connected with pneumatic cylinder (11), the cylinder body of pneumatic cylinder (11) is fixedly connected with the outer end of cutting cylinder (1) through a support, the piston rod of pneumatic cylinder (11) is fixedly connected with the top end of pressing plate (10), and cutting plate (2) is a grid structure composed of a plurality of blades, and the rotor of motor (8) is slidingly connected with rotating shaft (3) through a key groove.

3. The stem cell supernatant automated preparation device according to claim 2, characterized in that: The outer end of rotating shaft (3) is fixedly connected with uniform distribution disc (12) at the position of the bottom end of cutting knife (4), the uniform distribution disc (12) is a conical structure, and the tip of the conical structure is arranged close to cutting knife (4), and the top end of cutting knife (4) is arranged in contact with the bottom end of cutting plate (2).

4. The stem cell supernatant automated preparation device according to claim 3, characterized in that: The outer end diameter of uniform distribution disc (12) is equal to the inner wall diameter of the top end of separation cylinder (6), a sealing ring (13) is arranged between cutting cylinder (1) and separation cylinder (6), the top end of sealing ring (13) is fixedly connected with the bottom end of cutting cylinder (1), the bottom end of sealing ring (13) is rotatably connected with the top end of separation cylinder (6), and sealing ring (13) is made of flexible material.

5. The stem cell supernatant automated preparation device according to claim 2, characterized in that: The outer end of rotating shaft (3) is fixedly connected with stirring rod (14) between uniform distribution disc (12) and filter box (5), and stirring rod (14) is apart from the top end of the inner wall of filter box (5) by two to three centimeters.

6. The stem cell supernatant automated preparation device according to claim 5, characterized in that: The outer end of separation cylinder (6) is fixedly connected with separation ring (15) close to the bottom end thereof, separation ring (15) is communicated with the inner wall of separation cylinder (6), and a plurality of upper quick connectors (16) are fixedly connected with the bottom end of separation ring (15).

7. The stem cell supernatant automated preparation device according to claim 6, characterized in that: A lower quick connector (17) corresponding to each upper quick connector (16) is fixedly connected with water bath tank (7) at a position corresponding to upper quick connector (16), the bottom end of lower quick connector (17) is communicated with external equipment through a pipeline, and each lower quick connector (17) is arranged in pairs with the corresponding upper quick connector (16).

8. The stem cell supernatant automated preparation device of claim 1, wherein: The piezoelectric stack (9) bottom fixedly connected with spring (18), the spring (18) bottom fixedly connected with base (19), the cutting cylinder (1), water bath box (7) and motor (8) are all with base (19) fixedly connected, the fixedly connected with a plurality of one-way spray head on the pressing plate (10), and a plurality of spray heads are connected with external system.

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