Cell culture counting plate

Abstract

The utility model provides a cell culture counting plate and relates to the technical field of cell culture. A plurality of mounting grooves, wherein the plurality of mounting grooves are uniformly formed in the base plate; the plurality of counting chambers are in one-to-one correspondence and are arranged in the plurality of mounting grooves in a volume variable manner; and the sealing plate is tightly arranged on the base plate in a covering manner so as to seal and cover the plurality of counting chambers. According to the counting plate, the problems that the counting chamber structure of the existing counting plate is fixed and is not suitable for cells with different volumes and the cells need to be taken out for counting during observation are solved, and the counting chamber is arranged and is adjusted in the mounting groove, so that the accommodating cavity of the counting chamber is changed, and the cells with different volumes can be conveniently used.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field especially relates to a cell culture counting plate. BACKGROUND

[0002] In the field of cell biology research, biopharmaceuticals, etc., cell culture and counting are very important experimental operations. Cell counting plate is a commonly used cell counting tool, and the traditional cell counting plate is usually made of a specially made glass with specific counting chambers engraved on it. However, the existing cell culture counting plate has some shortcomings in use.

[0003] On the one hand, the counting chamber structure of the traditional counting plate is relatively fixed, and it is difficult to adjust the counting flexibly for different types and sizes of cells, which affects the accuracy of counting. For example, for some smaller cells, the cell distribution may be uneven due to the inappropriate size of the counting chamber, which affects the counting result.

[0004] On the other hand, in the process of cell culture, the existing counting plate is not convenient for real-time observation and monitoring of cells, and the cells need to be taken out from the culture environment for counting operation, which not only increases the complexity of experimental operation, but also may affect the activity and state of cells due to the change of the environment, thereby affecting the subsequent experimental results. SUMMARY

[0005] The utility model aims at providing a cell culture counting plate, which solves the problem of fixed counting chamber structure of the existing counting plate, which is not suitable for different sizes of cells and needs to be taken out for counting during observation.

[0006] The above technical purpose of the utility model is realized by the following technical scheme:

[0007] A cell culture counting plate comprises:

[0008] A substrate;

[0009] A plurality of mounting grooves are evenly provided on the substrate;

[0010] A plurality of counting chambers are one-to-one corresponding and variably provided in the mounting grooves;

[0011] A sealing plate is tightly provided on the substrate to seal and cover the counting chambers.

[0012] According to the cell culture counting plate provided by the utility model: the substrate comprises:

[0013] A placing plate is used for placing cultured cells;

[0014] A magnetic block is arranged at one end of the placement plate away from the counting chamber.

[0015] A ring-shaped anti-overflow groove is arranged at the edge of the placement plate away from the magnetic block.

[0016] The installation groove is arranged on the placement plate for placing the counting chamber.

[0017] According to the cell culture counting plate, the installation groove comprises:

[0018] A plurality of installation cavities are evenly arranged on the base plate.

[0019] A plurality of moving grooves are circularly arranged along the edge of the installation cavity.

[0020] An adjusting groove is arranged at the edge of the moving groove and linearly arranged at the edge of the moving groove.

[0021] A sliding groove is arranged at the bottom of the installation cavity, and the sliding groove is correspondingly arranged with the moving groove.

[0022] According to the cell culture counting plate, the counting chamber comprises:

[0023] A plurality of moving side walls are movably arranged in the installation cavity.

[0024] A culture groove is correspondingly arranged on the moving side wall.

[0025] An adjusting plate is fixedly connected to one end of the moving side wall away from the culture groove, and the adjusting plate is arranged in the moving groove.

[0026] A limiting strip is detachably embedded at one end of the adjusting plate away from the moving side wall, and the limiting strip is arranged in the adjusting groove.

[0027] The moving side wall moves in the moving groove corresponding to the adjusting plate, and the volume of the culture groove is changed.

[0028] According to the cell culture counting plate, the moving side wall is provided with a sliding block corresponding to the sliding groove at one end close to the bottom of the installation cavity, and the sliding block is slidably embedded in the sliding groove.

[0029] According to the cell culture counting plate, the sealing plate comprises:

[0030] A transparent cover plate is tightly covered on the base plate corresponding to the plurality of counting chambers;

[0031] A transparent sealing gasket is arranged on the transparent cover plate corresponding to the plurality of counting chambers at one end close to the counting chambers;

[0032] A pressing suction disc is arranged on the transparent cover plate at one end close to the counting chambers, and the pressing suction disc is distributed between the transparent sealing gaskets;

[0033] A sealing ring is arranged on the transparent cover plate at the edge of the end surface of the transparent sealing gasket;

[0034] The transparent cover plate seals the cultured cells and prevents the cells from contacting the external air during observation.

[0035] Therefore, the present application has the following beneficial technical effects:

[0036] The counting chamber is arranged in the mounting groove, so that the size of the culture tank inside the counting chamber can be changed. Since the volume of the culture tank inside the counting chamber can be changed, the counting chamber is better suitable for counting cells of different sizes, and the cells are more uniformly distributed. Meanwhile, the sealing plate is sealingly arranged on the mounting groove and the counting chamber, the counting chamber containing the cells is sealed by the sealing plate, so that the cells are prevented from contacting the external air during observation, thereby ensuring the state and activity of the cells and making the experimental results more accurate.

[0037] The additional aspects and advantages of the present application will be partially given in the following description, partially become obvious from the following description, or be understood through the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0038] In order to more clearly illustrate the technical solutions in the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of these drawings.

[0039] Figure 1 is a schematic diagram of the overall structure of the embodiment of the present application;

[0040] Figure 2 is a schematic diagram of the bottom of the overall structure of the embodiment of the present application;

[0041] Figure 3 is a top plan view of the base plate of the embodiment of the present application;

[0042] Figure 4 is a base plate bottom plan view of the embodiment of the utility model;

[0043] Figure 5 is a base plate overall structure schematic view of the embodiment of the utility model;

[0044] Figure 6 is a counting chamber structure schematic view of the embodiment of the utility model;

[0045] Figure 7 is a counting chamber bottom schematic view of the embodiment of the utility model;

[0046] Figure 8 is Figure 5 enlarged view of A in the figure.

[0047] Reference signs:

[0048] 10, base plate; 11, placing plate; 12, magnetic block; 13, annular anti-overflow groove;

[0049] 20, installation groove; 21, installation cavity; 22, moving groove; 23, adjusting groove; 24, sliding groove;

[0050] 30, counting chamber; 31, moving side wall; 32, culture groove; 33, adjusting plate; 34, limiting strip;

[0051] 311, sliding block;

[0052] 40, sealing plate; 41, transparent cover plate; 42, transparent sealing gasket; 43, extrusion suction disc; 44, sealing ring. Specific embodiments

[0053] In order to make the purpose, technical scheme and advantages of the utility model more clear, the technical scheme in the utility model will be described clearly and completely below by combining with the drawings in the utility model, obviously, the described embodiment is a part of the embodiment of the utility model, rather than all the embodiments. Based on the embodiment in the utility model, all other embodiments obtained by the person skilled in the art without creative labor are within the protection scope of the utility model.

[0054] In the description of the embodiments of this utility model, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model. In addition, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0055] In the description of the embodiments of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this utility model based on the specific circumstances.

[0056] In this embodiment of the utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0057] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0058] The following is combined Figures 1-8 The embodiments shown illustrate the technical solution of this utility model:

[0059] The utility model provides a cell culture counting plate, including base plate 10, a plurality of installation groove 20, a plurality of installation groove 20 evenly are set up on base plate 10, a plurality of counting chamber 30, a plurality of counting chamber 30 correspondingly and volume variablely set up in a plurality of installation groove 20, sealing plate 40, sealing plate 40 tightly cover sets up on base plate 10 to seal the covering of a plurality of counting chamber 30.

[0060] It can be understood that the base plate 10 is rectangular, circular or other special-shaped structure, and the base plate 10 is made of transparent material (such as optical glass or medical-grade polystyrene), and a plurality of installation grooves 20 are evenly arranged on the base plate 10, the counting chamber 30 is placed in the installation groove 20, the counting chamber 30 is used to place the cultured cells, the cells are uniformly distributed in the plurality of counting chambers 30, and the number of cells in each counting chamber 30 is observed in turn, and the corresponding counting chamber 30 is laid on the base plate 10, and the sealing plate 40 seals the counting chamber 30 on the base plate 10, so that the cells in the counting chamber 30 are not in contact with the outside air during observation, thereby better protecting the survival of the cells, and the volume of the counting chamber 30 can be adjusted, thereby being more suitable for cells of different volumes, so that the use efficiency of the counting plate is improved, and the application range is wider.

[0061] The cell culture counting plate provided by the embodiment of the utility model, the counting chamber 30 of the cultured cell is placed by the installation groove 20 arranged, the sealing plate 40 is sealed and placed above the counting chamber 30, so that the cells in the counting chamber 30 are sealed and placed, and the cells are not in contact with the external air during counting, thereby improving the accuracy of cell counting, the size of the inner cavity of the counting chamber 30 is adjusted by moving the inside of the counting chamber 30, so that the counting chamber 30 can better conform to cells of different volumes, thereby making the application range of the counting plate more extensive.

[0062] According to the cell culture counting plate provided by the embodiment of the utility model, the base plate 10 comprises: a placement plate 11 for placing cultured cells; a magnetic block 12 arranged at one end of the placement plate 11 away from the counting chamber 30; and a ring-shaped anti-overflow groove 13 arranged at the edge of the placement plate 11 away from the magnetic block 12; wherein the installation groove 20 is arranged on the placement plate 11 for placing the counting chamber 30.

[0063] Figure 1 And Figure 2The utility model discloses a cell culture counting plate, base plate 10 is formed by the combination of placing plate 11, magnetic block 12 and annular anti -overflow groove 13, and the annular anti -overflow groove 13 divides placing plate 11 into inner placing plate 11 and outer placing plate 11 two parts, and the magnetic block 12 is arranged at the bottom four corners of outer placing plate 11 and is adsorbed on the microscope when placing plate 11 is placed on the microscope, so that placing plate 11 is fixed, and the microscope is observed conveniently, and the mounting groove 20 is arranged on inner placing plate 11, and the mounting groove 20 is separated from outer placing plate 11 through annular anti -overflow groove 13, so that when the cell in the counting chamber 30 in the mounting groove 20 overflows the counting chamber 30, it does not flow down along outer placing plate 11, and the edge of placing plate 11 is cleaner, and the microscope is observed better, and the annular anti -overflow groove 13 can collect the overflowed cell, and the subsequent processing work is convenient, and the counting is more accurate and clean.

[0064] According to the cell culture counting plate provided by the utility model, the mounting groove 20 comprises a plurality of mounting cavities 21, the plurality of mounting cavities 21 are evenly arranged on the base plate 10, a plurality of moving grooves 22, the plurality of moving grooves 22 are circularly arranged along the edges of the mounting cavities 21, an adjusting groove 23, the adjusting groove 23 is arranged at the edges of the moving grooves 22 and is linearly arranged at the edges of the moving grooves 22, and a sliding groove 24, the sliding groove 24 is arranged at the bottom of the mounting cavity 21, and the sliding groove 24 is correspondingly arranged with the moving grooves 22.

[0065] Figure 3 、 Figure 5 and Figure 8 The utility model discloses a cell culture counting plate, and the mounting groove 20 is formed by the combination of the mounting cavity 21, the moving groove 22, the adjusting groove 23 and the sliding groove 24, the mounting cavity 21 is evenly arranged on the base plate 10, each mounting cavity 21 is circular structure, and the size is same and uniformly linearly arranged on the inner placing plate 11 of the base plate 10, the arrangement horizontal number of the mounting cavity 21 on the inner placing plate 11 is determined according to the size of the inner placing plate 11, the area of the inner placing plate 11 is large, so the number of arrangement horizontal number is more, and the area is small, so the number of arrangement horizontal number is less, four moving grooves 22 are evenly arranged at the circular edges of each mounting cavity 21, the four moving grooves 22 are circularly arranged, a plurality of adjusting grooves 23 are linearly arranged at the horizontal edges of the four moving grooves 22, the distance between each adjusting groove 23 is same, and the sliding groove 24 is arranged at the inner bottom of the mounting cavity 21 opposite the moving groove 22, the moving groove 22 and the sliding groove 24 are on the same vertical line, so that the mounting and placing of the counting chamber 30 are facilitated, and the size of the counting chamber 30 is adjusted.

[0066] According to the cell culture counting plate provided by the embodiment of the utility model, the counting chamber 30 comprises a plurality of moving side walls 31, the plurality of moving side walls 31 are movably arranged in the mounting cavity 21; the culture tank 32 is arranged on the plurality of moving side walls 31 correspondingly; the adjusting plate 33 is fixedly connected to one end of the moving side wall 31 away from the culture tank 32, and the adjusting plate 33 is arranged in the moving groove 22; the limiting strip 34 is detachably embedded in one end of the adjusting plate 33 away from the moving side wall 31, and the limiting strip 34 is arranged in the adjusting groove 23; wherein the moving side wall 31 slides in the moving groove 22 correspondingly to the movement of the adjusting plate 33, so that the volume of the culture tank 32 is changed.

[0067] Figure 6 And Figure 7 A cell culture counting plate is implemented in the embodiment, the counting chamber 30 is formed by the combination of the moving side wall 31, the culture tank 32, the adjusting plate 33 and the limiting strip 34, the moving side wall 31 is in arc structure, the plurality of moving side walls 31 are combined to form a complete circular structure, and the moving side wall 31 is arranged in the mounting cavity 21, the culture tank 32 is arranged in the circular structure formed by the combination of the plurality of moving side walls 31, the culture tank 32 is a cylindrical container made of flexible and scalable transparent material (for example, ultra-thin flexible glass), the side wall of the culture tank 32 is fixed with the moving side wall 31, so that the two are pasted together, so that the culture tank 32 is stretched and expanded or contracted by moving the moving side wall 31, that is, the volume of the culture tank 32 is changed, the adjusting plate 33 is fixedly arranged outside the arc end of the moving side wall 31, and is arranged in the moving groove 22 on the placing plate 11, so that the adjusting plate 33 moves horizontally in the moving groove 22, the limiting strip 34 is embedded at one end of the adjusting plate 33 away from the moving side wall 31, and the limiting strip 34 is in long strip structure and is detachably embedded in the adjusting plate 33, when the adjusting plate 33 is arranged in the moving groove 22, the limiting strip 34 is embedded in the adjusting plate 33 and the adjusting groove 23, so that the sliding position of the adjusting plate 33 is fixed, and the moving position of the moving side wall 31 is determined, so that the size of the culture tank 32 is determined, since the adjusting groove 23 is provided with a plurality of sliding grooves 24, when the limiting strip 34 is arranged in the adjusting groove 23, the adjusting plate 33 is limited by the limiting strip 34, so that the moving position of the moving side wall 31 is limited, and the volume of the culture tank 32 is determined.

[0068] According to the cell culture counting plate provided by the embodiment of the utility model, the plurality of moving side walls 31 are provided with sliding blocks 311 corresponding to the sliding grooves 24 at one end close to the bottom of the mounting cavity 21, and the sliding blocks 311 are slidably embedded in the sliding grooves 24.

[0069] Figure 7 And Figure 8The cell culture counting plate is characterized in that: a sliding block 311 is arranged on the end of the movable side wall 31 close to the bottom of the mounting cavity 21 corresponding to the sliding groove 24, and the sliding block 311 is arranged in the sliding groove 24, so that the movement of the movable side wall 31 is kept in the same horizontal straight line and cannot deviate, thereby stabilizing the expansion and contraction of the culture groove 32, and the expansion and contraction of the culture groove 32 is more convenient.

[0070] According to the cell culture counting plate provided in the embodiment of the utility model, the sealing plate 40 comprises a transparent cover plate 41, the transparent cover plate 41 is tightly attached to the base plate 10 corresponding to the plurality of mounting grooves 20; a transparent sealing gasket 42, the transparent sealing gasket 42 is arranged on the end of the transparent cover plate 41 close to the mounting groove 20; a squeeze suction disc 43, the squeeze suction disc 43 is arranged on the end of the transparent cover plate 41 close to the mounting groove 20, and the squeeze suction disc 43 is distributed between the transparent sealing gaskets 42; a sealing ring 44, the sealing ring 44 is arranged at the edge of the end face of the transparent sealing gasket 42 on the transparent cover plate 41; wherein the transparent cover plate 41 seals the cultured cells, and the contact with the external air is isolated during observation.

[0071] Figure 1 And Figure 2 The cell culture counting plate is characterized in that: a sliding block 311 is arranged on the end of the movable side wall 31 close to the bottom of the mounting cavity 21 corresponding to the sliding groove 24, and the sliding block 311 is arranged in the sliding groove 24, so that the movement of the movable side wall 31 is kept in the same horizontal straight line and cannot deviate, thereby stabilizing the expansion and contraction of the culture groove 32, and the expansion and contraction of the culture groove 32 is more convenient.

[0072] Usage process:

[0073] In use, according to the size of the cell volume, the counting chamber 30 is adjusted, the culture tank 32 is pushed or pulled by the moving side wall 31 in the counting chamber 30, so that the culture tank 32 is expanded or contracted, and the volume of the cells placed in the counting chamber 30 is adjusted, so that the cell culture counting of different volumes can be applied, so that the cells are more evenly distributed during counting, and the number of cells is better distinguished, secondly, after the cells are placed, the transparent cover plate 41 is adsorbed on the placement plate 11 by the extrusion suction cup 43, during placement, the transparent sealing gasket 42 on the transparent cover plate 41 is placed in the counting chamber 30 on the placement plate 11 in turn, the counting chamber 30 is sealed, so that the transparent cover plate 41 is fixed on the placement plate 11, the counting chamber 30 with cells is sealed, so that the cells will not be in contact with the external air during culture counting, thereby better improving the overall counting effect, making the counting more accurate, secondly, the magnetic block 12 arranged at the bottom of the placement plate 11, the placement plate 11 is better fixed on the microscope stage through the magnetic block 12, which is convenient for observing and counting the cells through the microscope, thereby making the placement plate 11 placed on the microscope more stable, thereby better solving the problem that the volume of the counting chamber 30 cannot be adjusted and the cells are taken out during counting, so that the cells are in contact with the external air and the counting is inaccurate.

[0074] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A cell culture counting plate, characterized by, include: base(10); Multiple mounting slots (20) are evenly formed on the substrate (10); Multiple counting chambers (30) are arranged in a one-to-one correspondence and with variable volume in multiple mounting slots (20); A sealing plate (40) is tightly fitted over the substrate (10) to seal and cover the plurality of counting chambers (30).

2. A cell culture counting plate according to claim 1, wherein, The substrate (10) includes: Placement plate (11), said placement plate (11) is used to place cultured cells; A magnetic block (12) is disposed at one end of the placement plate (11) away from the counting chamber (30); An annular anti-overflow groove (13) is formed at one end edge of the placement plate (11) away from the magnetic block (12); The mounting slot (20) is provided on the placement plate (11) for placing the counting chamber (30).

3. A cell culture counting plate according to claim 1, wherein The mounting slot (20) includes: Multiple mounting cavities (21) are evenly formed on the substrate (10); Multiple movable slots (22) are arranged in a circular array along the edge of the mounting cavity (21); Adjustment groove (23), the adjustment groove (23) is disposed at the edge of the moving groove (22) and is distributed in a straight line array at the edge of the moving groove (22); A sliding groove (24) is formed at the bottom of the mounting cavity (21), and the sliding groove (24) is distributed correspondingly to the moving groove (22).

4. A cell culture counting plate according to claim 3, wherein The counting chamber (30) includes: Multiple movable sidewalls (31) are movably disposed in the mounting cavity (21); The culture tank (32) is correspondingly adhered to the plurality of movable sidewalls (31); An adjustment plate (33) is fixedly connected to one end of the movable sidewall (31) away from the culture tank (32), and the adjustment plate (33) is placed in the movable tank (22); A limiting strip (34) is detachably embedded in one end of the adjusting plate (33) away from the movable sidewall, and the limiting strip (34) is placed in the adjusting groove (23); The movable sidewall (31) moves in the movable groove (22) corresponding to the adjustment plate (33) to change the volume of the culture tank (32).

5. A cell culture counting plate according to claim 4, wherein Each of the multiple movable sidewalls (31) has a sliding block (311) at one end near the bottom of the mounting cavity (21) corresponding to the sliding groove (24), and the sliding block (311) is slidably embedded in the sliding groove (24).

6. A cell culture counting plate according to claim 1, wherein The sealing plate (40) includes: A transparent cover plate (41) is tightly covered on the substrate (10) corresponding to the plurality of counting chambers (30); A transparent sealing gasket (42) is disposed on the transparent cover plate (41) near one end of the counting chamber (30) corresponding to a plurality of the counting chambers (30); Squeezing suction cups (43) are arranged on one end of the transparent cover plate (41) close to the counting chamber (30), and the squeezing suction cups (43) are distributed between the transparent sealing pads (42); Sealing rings (44) are arranged at the edges of the end surface of the transparent sealing pads (42) on the transparent cover plate (41); The transparent cover plate (41) seals the cultured cells and isolates the cultured cells from the external air during observation.

Images