Microplate sample automatic detection device with shooting analysis function

Abstract

The utility model discloses an automatic microplate sample detection device with a shooting analysis function, which comprises a detection box, a detection table, a touch screen controller, a moving component, an electric heating plate for incubating a microplate, a sample adding component for adding a reagent to the microplate, and a shooting component for shooting the microplate, the electric heating plate is installed at the top end of the detection table, the touch screen controller is installed on the detection box, the sample adding assembly moves in the length direction of the detection table, the shooting assembly is arranged on one side of the detection table, and the moving assembly drives the microwell plate to move so as to be matched with the shooting assembly to shoot the microwell plate. According to the device, the processes of sample adding, incubation and the like of the microwell plate can be automatically completed, manual operation is not needed, the photographing assembly can automatically photograph the incubated microwell plate, and the photographed picture of the microwell plate can be directly transmitted to corresponding analysis software or an analyzer to be analyzed.

Description

TECHNICAL FIELD

[0001] The utility model relates to a test device especially a micro -hole board sample automatic detection device with shooting analysis function. BACKGROUND

[0002] The micro -hole board can be through the fixed specific biological molecule (such as enzyme, antigen or antibody) on the surface, is used to detect and determine the content, activity, affinity and the interaction with target molecule of drug etc. Micro -hole board is often used in drug research and development and screening, life science research, medical examination, food detection etc. field.

[0003] The detection quality, detection level and detection method of food safety detection technology will directly influence the final detection result. And in actual operation process, laboratory detection (such as liquid chromatography and mass spectrometry technique) cost is larger, and time is longer, and work efficiency is lower. Enzyme-linked immunosorbent assay and micro -hole biological chip based on the rapid detection technology of micro -hole board can carry out high throughput preliminary screening detection to food safety in shorter time. But in the detection process, because sample quantity is large, manual multi-step liquid adding, it is easy to appear omission, wrong addition or multiple addition etc. And in the detection process, need to add sample, incubation on different instruments, and when subsequent analysis, still need to transfer the micro -hole board of adding sample, incubation to analysis equipment and analyze, inconvenient actual use. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of automatic detection device with the functions such as sample adding, incubation, shooting analysis etc.

[0005] Technical scheme: the micro -hole board sample automatic detection device with shooting analysis function of the utility model, including detection box and detection table, the detection table is fixed at the bottom in detection box, still including touch screen controller, moving assembly, incubation micro -hole board's electric heating plate, sample adding assembly to micro -hole plate and add reagent, shooting component for shooting micro -hole plate, the electric heating plate is installed at the top of detection table, the touch screen controller is installed on detection box, the sample adding assembly moves along the length direction of detection table, the shooting component is arranged at one side of detection table, the moving assembly drives micro -hole plate to move to cooperate shooting component and shoot micro -hole plate, the electric heating plate, sample adding assembly, shooting component and moving assembly are electrically connected with touch screen controller respectively.

[0006] Further, the sample adding assembly includes a pipetting part and a moving part, the pipetting part is installed on the moving part and moves synchronously with the moving part, the moving part includes a first motor, a first guide rail installed inside the detection box, and a mounting bracket slidably connected with the first guide rail, the detection table penetrates through the through hole of the mounting bracket, the output shaft of the first motor is fixedly connected with a first lead screw, and the first lead screw is threadedly connected with the mounting bracket.

[0007] Further, the pipetting part comprises a TIP head adapter for connecting a TIP head, a piston matched with the TIP head adapter, a connecting frame slidingly connected to the inner side of the mounting frame, an installation body fixedly connected to the inner wall of the connecting frame, and the TIP head adapter is fixedly connected to the installation body, a moving plate slidingly connected to the inner side of the connecting frame, and the piston is fixedly connected to the bottom end of the moving plate.

[0008] Further, the top end of the mounting frame is fixedly connected with a second motor, the output shaft of the second motor is fixedly connected with a second lead screw, the second lead screw is threadedly connected with the connecting frame, the top end of the moving plate is fixedly connected with a third motor, the output shaft of the third motor is fixedly connected with a third lead screw, and the third lead screw is threadedly connected with the moving plate.

[0009] Further, the bottom end of the connecting frame is slidingly connected with a pushing frame, and the two vertical rods of the pushing frame extend above the installation body.

[0010] Further, the shooting assembly comprises a gantry body, a fourth motor fixedly connected with the gantry body, and a fourth lead screw fixedly connected with the output shaft of the fourth motor, the gantry body is fixedly installed at the bottom of the detection box, the outer periphery of the fourth lead screw is threadedly connected with a linkage block, a second guide rail is installed on the gantry body, and the linkage block moves along the second guide rail.

[0011] Further, the shooting assembly further comprises an upper support fixedly connected to the bottom end of the linkage block, and a lower support fixedly connected to the bottom end of the upper support, a camera is installed in the groove of the upper support, and an LED lamp is installed in the groove of the lower support.

[0012] Further, a light homogenizing plate is further installed on the upper support, and the light homogenizing plate is located directly above the LED lamp.

[0013] Further, the moving assembly comprises a fifth motor, a transmission belt, and a tray on which a microplate is placed, the tray is slidingly supported at the top end of the electric heating plate, one side of the detection table is rotationally connected with a belt pulley, the output shaft of the fifth motor is fixedly connected with another belt pulley, the transmission belt is transmissionally sleeved on the outer periphery of the two belt pulleys, one side of the tray is provided with a connecting portion, and the belt pulley drives the tray to move through the connecting portion.

[0014] Further, the top end of the detection table is sequentially provided with a waste box, a TIP head box, and a liquid storage box, the waste box is used for placing discarded TIP heads, the TIP head box is used for placing TIP heads to be replaced, and the liquid storage box is used for storing reagents to be added.

[0015] Beneficial effects: compared with the prior art, the utility model has the advantages that: can automatically complete the microplate detection experiment, does not need manual operation, when facing a large number of detection samples, can reduce the failure caused by manual operation, the device can realize the sample adding, incubation and other processes of the microplate, and the shooting assembly can automatically shoot the microplate after incubation, the picture of the shot microplate can be directly transmitted to the corresponding analysis software or analyzer to carry out analysis, and the microplate does not need to be transferred in different instruments during the detection experiment process. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the structure schematic diagram of the utility model.

[0017] Figure 2 It is another structure schematic diagram of the utility model.

[0018] Figure 3 It is the structure schematic of the detection box inside Figure 1 .

[0019] Figure 4 It is the structure schematic of the detection box inside Figure 2 .

[0020] Figure 5 It is the structure schematic of the detection box inside Figure 3 .

[0021] Figure 6 It is the structure schematic of the detection box inside Figure 3 .

[0022] Figure 7 It is the split structure schematic diagram of the connecting frame, loading body and moving plate of the utility model.

[0023] Figure 8 It is the split structure schematic diagram of the loading body in Figure 7 .

[0024] Figure 9 It is the structure schematic diagram of the shooting assembly of the utility model.

[0025] Figure 10 It is the front view of the portal frame body of the utility model. DETAILED DESCRIPTION

[0026] The technical scheme of the utility model will be further described below in combination with the drawings.

[0027] Example 1

[0028] As Figures 1 to 8The utility model provides a kind of microplate sample automatic detection device with shooting analysis function, including detection box 1 and detection table 2, detection table 2 is fixed in the bottom of detection box 1, and detection table 2 is located inside detection box 1, microplate sample automatic detection device with shooting analysis function further include touch screen controller 3, touch screen controller 3 is installed on detection box 1, touch screen controller 3 the side of detection box 1 where is installed with box door 12, the back of detection box 1 is installed with power interface 13, and power switch 14 is installed on detection box 1.

[0029] Microplate sample automatic detection device with shooting analysis function further include moving assembly, incubation microplate's electric heating plate 4, reagent adding assembly to microplate, shooting assembly for shooting microplate, the top of detection table 2 is sequentially provided with waste box 8, TIP head box 9 and liquid storage box 10, electric heating plate 4 is installed at the top of detection table 2;Reagent adding assembly moves along the length direction of detection table 2, reagent adding assembly moves to the top of microplate after sucking reagent from liquid storage box 10, then the reagent of sucking is added to the hole corresponding to microplate;Moving assembly can drive microplate to move at the top of detection table 2, when microplate moves to the top of electric heating plate 4, electric heating plate 4 can heat incubation processing to microplate, shooting assembly is arranged at one side of detection table 2, and shooting assembly can be connected with corresponding analysis software or analyzer by wireless connection mode in actual use, and microplate picture is transmitted to analysis software or analyzer for analysis;Wherein electric heating plate 4, reagent adding assembly, shooting assembly and moving assembly are electrically connected with touch screen controller 3, and the opening and movement of electric heating plate 4, reagent adding assembly, shooting assembly and moving assembly are controlled by touch controller 3 in actual detection, and electric heating plate 4, reagent adding assembly, shooting assembly and moving assembly are all located inside detection box 1, and the detection process of microplate is carried out in relatively closed space, to avoid the influence of external factors on detection.

[0030] Wherein moving assembly includes fifth motor 701, transmission belt 702 and tray 703 of placing microplate, tray 703 is slidably supported at the top of electric heating plate 4, detection table 2 is rotatably connected with pulley at one side, another pulley is fixedly connected with the output shaft of fifth motor 701, transmission belt 702 is transmission sleeve connected on the outer periphery of two pulleys, the side of tray 703 is provided with connecting part 704, pulley drives tray 703 to move through connecting part 704, fifth motor 701 is also set as positive and negative motor, fifth motor 701 can drive tray 703 to move at the top of detection table 2 when starting.

[0031] The sample adding assembly comprises a pipetting part and a moving part, the pipetting part is installed on the moving part and moves synchronously with the moving part, the moving part comprises a first motor 501, a first guide rail 502 installed inside the detection box 1, and a mounting frame 503 in sliding connection with the first guide rail 502, the detection table 2 penetrates through the through hole of the mounting frame 503, the output shaft of the first motor 501 is fixedly connected with a first lead screw 504, the first lead screw 504 is in threaded connection with the mounting frame 503, and the mounting frame 503 moves along the first guide rail 502 when the first motor 501 is started. The first motor 501 is a reversible motor, so that the mounting frame 503 can move back and forth along the first guide rail 502.

[0032] The pipetting part comprises a TIP head adapter 505 for connecting a TIP head, and a piston 506 matched with the TIP head adapter 505, the inner side of the mounting frame 503 is in sliding connection with a connecting frame 507, the inner wall of the connecting frame 507 is fixedly connected with a loading body 508, and the TIP head adapter 505 is fixedly connected with the loading body 508. The inner side of the connecting frame 507 is in sliding connection with a moving plate 509, the moving plate 509 is located above the loading body 508, and the piston 506 is fixedly connected to the bottom end of the moving plate 509. The bottom end of the piston 506 extends to the inner cavity of the TIP head adapter 505; wherein the top end of the mounting frame 503 is fixedly connected with a second motor 510, the output shaft of the second motor 510 is fixedly connected with a second lead screw 511, the second lead screw 511 is in threaded connection with the connecting frame 507, and a vertical guide rod 515 is fixedly installed on the mounting frame 503. The connecting frame 507 moves up and down along the guide rod 515, and the second motor 510 is also a reversible motor. When the second motor 510 is started, the connecting frame 507 moves upward or downward; the top end of the moving plate 509 is fixedly connected with a third motor 512, the output shaft of the third motor 512 is fixedly connected with a third lead screw 513, the third lead screw 513 is in threaded connection with the moving plate 509, and the third motor 512 is also a reversible motor. When the third motor 512 is started, the moving plate 509 moves upward or downward along the inner wall of the connecting frame 507; the second motor 510 drives the connecting frame 507 to move upward or downward, the TIP head moves upward or downward following the connecting frame 507, so that the TIP head moves downward into the liquid storage box 10, the third motor 512 drives the moving plate 509 to move upward, and the piston 506 moves upward following the moving plate 509 synchronously, so that the TIP head sucks the reagent, then the second motor 510 drives the connecting frame 507 to move upward and away from the liquid storage box 10, then the first motor 501 drives the mounting frame 503 to move along the first guide rail 502, so that the pipetting part moves above the microplate, then the third motor 512 drives the moving plate 509 to move downward, and the piston 506 moves downward following the moving plate 509 synchronously, so that the TIP head sucks the reagent and is put into the microplate.

[0033] Preferably, the bottom end of the connecting frame 507 is slidingly connected with a push-out frame 514, and the two vertical rods of the push-out frame 514 extend above the loading body 508, the lateral part of the push-out frame 514 is sleeved on the outer periphery of the TIP head adapter 505, and the moving plate 509 can press downwardly the push-out frame 514 when moving downwardly (when in the initial position, a certain space is reserved between the two vertical rods of the push-out frame 514 and the moving plate 509, so as to prevent affecting the normal suction and placement of the TIP head, and the spring sleeved on the outer periphery of the two vertical rods of the push-out frame 514 can reset the push-out frame 514 when the acting force disappears), and the TIP head can be pushed out and removed when the push-out frame 514 is continuously pressed downwardly; similarly, the first motor 501 and the second motor 510 can be used in cooperation to enable the TIP head adapter 505 to automatically install the TIP head into the TIP head box 9 and automatically remove the TIP head to be replaced into the waste box 8.

[0034] Embodiment 2

[0035] As shown in Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 9 and Figure 10 , this embodiment is based on the previous embodiment, and the shooting assembly comprises a portal frame body 601, a fourth motor 602 fixedly connected with the portal frame body 601, and a fourth lead screw 603 fixedly connected with an output shaft of the fourth motor 602. An end of the fourth lead screw 603 away from the fourth motor 602 is rotatably connected with the portal frame body 601 through a bearing. The portal frame body 601 is fixedly installed at the bottom of the detection box. The fourth lead screw 603 is threadedly connected with a linkage block 604. The portal frame body 601 is provided with a second guide rail 605. The linkage block 604 moves along the second guide rail 605. The fourth motor 602 is a reversible motor, and the fourth motor 602 can drive the linkage block 604 to reciprocatingly move along the second guide rail 605.

[0036] The shooting assembly further comprises an upper support 606 fixedly connected at the bottom end of the linkage block 604, and a lower support 607 fixedly connected at the bottom end of the upper support 606. A camera 608 is installed in a recess of the upper support 606. An LED lamp 609 is installed in a recess of the lower support 607. The camera 608 shoots the microplate picture in actual use, and the LED lamp 609 provides illumination when shooting.

[0037] Working process: The device is controlled by the touch screen controller 3 to automatically detect, automatically assemble the TIP head, when the first motor 501 moves the mounting frame 503 to the top of the TIP head box 9, and then the second motor 510 moves the connecting frame 507 downward, finally the TIP head adapter 505 automatically installs the TIP head inside the TIP head box 9; when automatically sampling, the first motor 501 moves the mounting frame 503 to the top of the liquid storage box 10, the second motor 510 drives the connecting frame 507 to move downward, so that the TIP head moves downward into the liquid storage box 10, the third motor 512 drives the moving plate 509 to move upward, the piston 506 follows the moving plate 509 to move upward synchronously, and then the TIP head sucks the reagent, then the second motor 510 drives the connecting frame 507 to move upward and leave the liquid storage box 10, then the first motor 501 drives the mounting frame 503 to move along the first guide rail 502, so that the pipetting part moves to the top of the microplate, then the third motor 512 drives the moving plate 509 to move downward, the piston 506 follows the moving plate 509 to move downward synchronously, and the TIP head sucks the reagent into the microplate; when automatically incubating, the fifth motor 701 drives the tray 703 to move at the top of the detection table 2, so that the microplate moves to the top of the electric heating plate 4, and the electric heating plate 4 incubates the microplate; when taking pictures of the microplate after incubation, the fifth motor 701 drives the tray 703 to move between the upper bracket 606 and the lower bracket 607, the LED lamp 609 irradiates the microplate from below, and the camera 608 takes pictures of the microplate from above; when different holes of the microplate need to be taken, the fourth motor 602 is adjusted to move the linkage block 604, the upper bracket 606 and the lower bracket 607 to the specified position, and the pictures taken by the camera 608 are transmitted to the corresponding analysis software or analyzer for analysis; automatically disassemble the used TIP head, the first motor 501 moves the mounting frame 503 to the top of the waste box 8, the third motor 512 drives the moving plate 509 to press the push-out frame 514 downward, and when the push-out frame 514 continuously moves downward, the TIP head can be pushed out, and the used TIP head falls into the waste box 8.

Claims

1. A microplate sample automatic detection device with shooting analysis function, comprising a detection box (1) and a detection table (2), the detection table (2) is fixed on the bottom of the detection box (1), characterized in that: Also include touch screen controller (3), mobile components, incubation microplate electric heating plate (4), adding reagent to the microplate sample adding assembly, taking pictures of the microplate shooting assembly, the electric heating plate (4) is installed at the top of the detection table (2), the touch screen controller (3) is installed on the detection box (1), the shooting assembly is arranged on one side of the detection table (2), the sample adding assembly moves along the length direction of the detection table (2), the mobile assembly drives the microplate to move to cooperate with the shooting assembly to shoot the microplate, and the electric heating plate (4), the sample adding assembly, the shooting assembly and the mobile assembly are electrically connected with the touch screen controller (3) respectively.

2. The microplate sample automated detection device with photographic analysis function according to claim 1, wherein: The sample adding assembly comprises a pipetting part and a moving part, the pipetting part is installed on the moving part and moves synchronously with the moving part, the moving part comprises a first motor (501), a first guide rail (502) installed inside the detection box (1) and a mounting frame (503) in sliding connection with the first guide rail (502), the detection table (2) penetrates through the through hole of the mounting frame (503), and the output shaft of the first motor (501) is fixedly connected with a first lead screw (504) in threaded connection with the mounting frame (503).

3. The microplate sample automated detection device with photographic analysis capability of claim 2, wherein: The pipetting part comprises a TIP head adapter (505) for connecting a TIP head and a piston (506) matched with the TIP head adapter (505), the inner side of the mounting frame (503) is in sliding connection with a connecting frame (507), the inner wall of the connecting frame (507) is fixedly connected with a loading body (508), the TIP head adapter (505) is fixedly connected with the loading body (508), the inner side of the connecting frame (507) is in sliding connection with a moving plate (509), and the piston (506) is fixedly connected to the bottom end of the moving plate (509).

4. The microplate sample automated detection device with photographic analysis capability of claim 3, wherein: The top end of the mounting frame (503) is fixedly connected with a second motor (510), the output shaft of the second motor (510) is fixedly connected with a second lead screw (511) in threaded connection with the connecting frame (507), the top end of the moving plate (509) is fixedly connected with a third motor (512), the output shaft of the third motor (512) is fixedly connected with a third lead screw (513) in threaded connection with the moving plate (509).

5. The microplate sample automated detection device with photographic analysis capability of claim 3 or 4, wherein: The bottom end of the connecting frame (507) is in sliding connection with a push-out frame (514), and the two vertical rods of the push-out frame (514) extend above the loading body (508).

6. The microplate sample automated detection device with photographic analysis capability of claim 1, wherein: The shooting assembly comprises a gantry body (601), a fourth motor (602) fixedly connected with the gantry body (601), and a fourth lead screw (603) fixedly connected with the output shaft of the fourth motor (602), the gantry body (601) is fixedly installed at the bottom of the detection box, the outer periphery of the fourth lead screw (603) is in threaded connection with a linkage block (604), the gantry body (601) is provided with a second guide rail (605), and the linkage block (604) moves along the second guide rail (605).

7. The microplate sample automated detection device with photographic analysis capabilities of claim 6, wherein: The photographing assembly further includes an upper support (606) fixedly connected to the bottom end of the linkage block (604) and a lower support (607) fixedly connected to the bottom end of the upper support (606), a camera (608) is mounted at the groove of the upper support (606), and an LED lamp (609) is mounted at the groove of the lower support (607).

8. The microplate sample automated detection device with photographic analysis capability of claim 7, wherein: A light homogenizing plate (610) is further mounted on the upper support (606) and located directly above the LED lamp (609).

9. The microplate sample automated detection device with photographic analysis capability of claim 1, wherein: The moving assembly includes a fifth motor (701), a transmission belt (702) and a tray (703) for placing a microplate, the tray (703) is slidingly supported at the top end of the electric heating plate (4), one side of the detection table (2) is rotationally connected with a belt pulley, the output shaft of the fifth motor (701) is fixedly connected with another belt pulley, the transmission belt (702) is transmissionally sleeved at the outer peripheries of the two belt pulleys, one side of the tray (703) is provided with a connecting portion (704), and the belt pulley drives the tray (703) to move through the connecting portion (704).

10. The microplate sample automated detection device with imaging analysis function according to any one of claims 1 to 4, wherein: The top end of the detection table (2) is sequentially provided with a waste box (8), a TIP head box (9) and a liquid storage box (10).

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