Totally-closed nucleic acid extraction and detection device

Abstract

The utility model discloses a full-closed nucleic acid extraction and detection device which comprises a purification mechanism, the purification mechanism comprises a fixed part, magnetic beads and a magnetic attraction body, a cracking cavity, at least one washing cavity, an elution cavity and a transfer channel are formed in the fixed part, and the transfer channel is communicated with the cracking cavity, the washing cavity and the elution cavity; the magnetic bead is arranged in the fixing part; the magnetic attraction body is arranged in the fixing part and can magnetically attract the magnetic beads, the magnetic attraction body can drive the magnetic beads to transfer among the transfer channel, the splitting cavity, the washing cavity and the elution cavity, and the size of the magnetic attraction body is larger than that of the magnetic beads. The magnetic attraction body can be matched with an external magnetic field to drive the magnetic beads to move, splitting decomposition, washing and elution are all carried out in the fixed part and are in a totally-closed environment, and external impurities are prevented from interfering with purification of nucleic acid; moreover, during nucleic acid purification, equipment does not need to enter the fixed part, so that the problem of nucleic acid leakage is avoided, nucleic acid leakage during detection is avoided, and the interference of nucleic acid leakage on other nucleic acid detection is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid detection technical field, concretely relates to a kind of totally enclosed nucleic acid extraction and detection device. BACKGROUND

[0002] Nucleic acid detection box is a kit for detecting the genetic material (nucleic acid) of viruses or bacteria and other microorganisms, and is usually used for detecting viral infectious diseases and genetic testing. It contains a series of reagents and equipment for extracting, amplifying and detecting nucleic acids in samples.

[0003] The working principle of nucleic acid detection box is usually based on the fluorescent quantitative PCR (Polymerase Chain Reaction) method. The detection principle is to use the unique gene sequence of the virus as the detection target, and through PCR amplification, the selected target DNA (Deoxyribo Nucleic Acid) sequence is exponentially increased. Each amplified DNA sequence can combine with a pre-added fluorescently labeled probe to produce a fluorescent signal. The more target genes amplified, the stronger the cumulative fluorescent signal. Therefore, nucleic acid detection determines whether there is a specific nucleic acid in the sample by detecting the accumulation of fluorescent signals.

[0004] Publication No. CN112852626A discloses a sample detection device, which includes a magnetic sleeve and a detection box. The detection box is provided with at least four cavities arranged side by side and not communicating with each other, and a sealing structure is provided at the top to seal all the cavities. The magnetic sleeve can pierce the sealing structure and extend into each cavity. The cavities include a lysis cavity for lysing samples, a magnetic bead cavity preloaded with magnetic beads, a washing cavity for washing, and an elution cavity for elution. The bottom of the elution cavity is fixed with a reaction tube, and the top of the reaction tube is sealed. The reaction tube is preloaded with detection reagents. Except for the magnetic bead cavity, the other cavities are preloaded with different reagents for sample extraction. When washing nucleic acid, the magnetic rod of the detection instrument is inserted into the magnetic sleeve, and the magnetic sleeve adsorbs the magnetic beads in the lysis cavity. The magnetic sleeve moves into the washing cavity together with the magnetic rod, the magnetic rod is withdrawn, the magnetic beads adsorbed on the magnetic sleeve are released, and the magnetic sleeve moves up and down in the washing cavity to mix the reagents and magnetic beads in the cavity. Then, the magnetic beads are transferred to another washing cavity for secondary cleaning by the same method.

[0005] In the detection box, when the nucleic acid is extracted, the magnetic sleeve is inserted into the lysis chamber, the washing chamber and the elution chamber through the sealing structure, then the magnetic force bar box is controlled to move the magnetic sleeve by the detection instrument, so that the magnetic beads pass through the lysis chamber, the washing chamber and the elution chamber in turn. However, when the magnetic sleeve pierces the sealing mechanism, the sample in the detection box is exposed to the air, and there is a risk of nucleic acid leakage. The leaked nucleic acid may contaminate the laboratory environment or other samples, resulting in false positive detection results and affecting the accuracy of diagnosis. Content of the utility model

[0006] The utility model discloses a kind of fully-closed nucleic acid extraction and detection device, solve the technical problem that nucleic acid leaks when detection box extracts nucleic acid in prior art by using magnetic sleeve to transfer nucleic acid to overcome the above technical deficiencies.

[0007] To achieve the above technical purpose, the utility model takes the following technical scheme:

[0008] The utility model provides a kind of fully-closed nucleic acid extraction and detection device, including purification mechanism, the purification mechanism includes:

[0009] Fixed part, lysis chamber, at least one washing chamber, elution chamber and transfer channel are formed in it, and the transfer channel is communicated with the lysis chamber, the washing chamber and the elution chamber;

[0010] Magnetic beads are built-in in the fixed part;And

[0011] Magnetic body is built-in in the fixed part, and can magnetically attract the magnetic beads, the magnetic body can drive the magnetic beads to transfer between the transfer channel, lysis chamber, washing chamber, elution chamber, and the volume of the magnetic body is greater than the magnetic beads.

[0012] In one embodiment, the fully-closed nucleic acid extraction and detection device further includes two at least one magnetic attraction piece, the magnetic attraction piece is arranged outside the fixed part, and is magnetically connected with the magnetic body.

[0013] In one embodiment, the fixed part is further formed with nucleic acid flow channel;

[0014] The fully-closed nucleic acid extraction and detection device further includes detection reagent mechanism, the detection reagent mechanism is detachably connected with the fixed part, and is formed with detection chamber containing detection reagent, and one end of the detection chamber is open;

[0015] When the detection reagent mechanism is connected with the fixed part, the detection chamber is communicated with the nucleic acid flow channel, and the purification mechanism can block the opening of the detection chamber.

[0016] In one of the embodiments, the purification mechanism and the detection reagent mechanism are detachably connected by a plug-in structure, and the plug-in structure can seal the opening of the detection cavity, and when the purification mechanism and the detection reagent mechanism are plugged in, the plug-in structure can be at least partially inserted into the detection cavity and pressurize the detection cavity.

[0017] In one of the embodiments, the purification mechanism further comprises:

[0018] a plug-in part connected to the fixed part; and

[0019] a paraffin layer connected to the plug-in part;

[0020] When the detection reagent mechanism and the purification mechanism are connected, the plug-in part and the paraffin layer are inserted into the detection cavity.

[0021] In one of the embodiments, the purification mechanism further comprises:

[0022] a sealing layer arranged in the transfer channel and detachably connected to the fixed part, and when the sealing layer is connected to the fixed part, the sealing layer blocks the communication between the lysis cavity, the washing cavity and the transfer channel.

[0023] In one of the embodiments, the fixed part is formed with a mounting cavity in communication with the transfer channel;

[0024] The purification mechanism further comprises:

[0025] a winding part rotatably arranged in the mounting cavity and connected to the sealing layer, and used for winding the sealing layer to unblock the communication between the lysis cavity, the washing cavity, the elution cavity and the transfer channel.

[0026] In one of the embodiments, a window is formed in the middle of the sealing layer, the size of the two ends of the sealing layer is at least partially smaller than the size of the window, and the two ends of the sealing layer pass through the window and are connected to the winding part.

[0027] In one of the embodiments, the lysis cavity, the washing cavity and the elution cavity are linearly arranged along a first direction, and the side walls of at least some of the cavities along the two sides of the first direction are smoothly connected to the inner walls of the cavities along other directions by arc surfaces.

[0028] In one of the embodiments, the nucleic acid flow channel has a sealing cavity;

[0029] The purification mechanism comprises:

[0030] A sealing part is built in the sealing cavity and sealingly cooperates with the inner wall of the sealing cavity to close the nucleic acid flow channel.

[0031] An elution plug is slidingly arranged in the sealing cavity, and the elution plug is used to push the sealing part away from the sealing cavity by the fluid in the elution cavity to open the nucleic acid flow channel.

[0032] Compared with the prior art, the full-closed nucleic acid extraction and detection device provided by the utility model, when in use, the purification mechanism purifies the nucleic acid in the sample, the lysing cavity is provided with a lysing solution, the sample enters the lysing cavity, the sample is lysed by the lysing solution, the magnetic body is driven to move by an external magnetic field or a special fluid driving mechanism, the magnetic body magnetically adsorbs the magnetic beads and drives the magnetic beads to move, the magnetic beads are first moved into the lysing cavity to adsorb the lysed nucleic acid, the magnetic beads are driven to move by the magnetic body and enter the washing cavity through the transfer channel, the washing cavity stores a washing solution, the washing solution can wash the nucleic acid adsorbed by the magnetic beads, and the washing solution is used to remove the impurities such as protein and salt ions possibly remaining on the magnetic beads.

[0033] The magnetic body can be driven to move by the magnetic rod of the existing detection instrument, or can be driven to move by the external magnetic field or the special fluid driving mechanism to ensure that the magnetic beads can smoothly move along the transfer channel.

[0034] The magnetic body can be driven to move by the magnetic rod of the existing detection instrument, or can be driven to move by the external magnetic field or the special fluid driving mechanism to ensure that the magnetic beads can smoothly move along the transfer channel. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 is the explosion view of the full-closed nucleic acid extraction and detection device provided by an embodiment of the utility model;

[0036] Figure 2 is the structure schematic view of the full-closed nucleic acid extraction and detection device provided by an embodiment of the utility model;

[0037] Figure 3is a sectional view of the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0038] Figure 4 is a structural schematic view of the second shell, the third shell and the detection reagent mechanism in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0039] Figure 5 is a structural schematic view of the second shell in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0040] Figure 6 is a structural schematic view of the second shell, the sealing layer and the winding part in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0041] Figure 7 is a structural schematic view of the second shell in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0042] Figure 8 is a structural schematic view of the third shell and the plug-in part in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0043] Figure 9 is a structural schematic view of the third shell, the plug-in part and the detection reagent mechanism in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0044] Figure 10 is a structural schematic view of the plug-in block, the plug-in strip and the paraffin layer in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0045] Figure 11 is a structural schematic view of the first shell in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model,

[0046] Figure 12 is a structural schematic view of the magnetic attraction piece and the fixing part in the fully-closed nucleic acid extraction and detection device provided by an embodiment of the utility model.

[0047] Mark explanation:

[0048] Purification mechanism 1;Nucleic acid flow channel 1a;Sealing cavity 1a1;Communication groove 1a2;Communication hole 1a3;

[0049] Fixed part 11; cracking cavity 11a; washing cavity 11b; elution cavity 11c; transfer channel 11d; communication channel 11e; mounting cavity 11f; first shell 111; first through hole 111a; second through hole 111b; second shell 112; first fixed hole 112a; second fixed hole 112b; first storage hole 112c; second storage hole 112d; liquid inlet channel 112e; third shell 113; puncture part 114;

[0050] Plug-in part 12; fixed ring 121; plug-in block 122; plug-in strip 123;

[0051] Paraffin layer 13; sealing layer 14; window 14a; winding part 15; magnetic body 16; sealing part 17; elution plug 18;

[0052] Sampling element 19; sample tube 191; sampling plug 192; closure plug 193;

[0053] Detection reagent mechanism 2; detection cavity 2a; fourth shell 21; detection shell 22;

[0054] Magnetic member 3. DETAILED DESCRIPTION

[0055] In order to make the purpose, technical scheme and advantages of the utility model clearer and more apparent, the utility model will be further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model and not to limit the utility model.

[0056] In order to solve the technical problem of nucleic acid leakage caused by the use of magnetic sleeve to transfer nucleic acid in the prior art, the utility model provides a fully-closed nucleic acid extraction and detection device, which can avoid nucleic acid leakage in the process of transferring nucleic acid.

[0057] Please refer to Figure 3 , Figure 3 The cross-sectional view of the fully-closed nucleic acid extraction and detection device provided by the embodiment of the utility model, the fully-closed nucleic acid extraction and detection device comprises a purification mechanism 1, the purification mechanism 1 comprises a fixed part 11, magnetic beads (not shown in the figure) and a magnetic body 16, the fixed part 11 is formed with a cracking cavity 11a, at least one washing cavity 11b, an elution cavity 11c and a transfer channel 11d, the transfer channel 11d communicates the cracking cavity 11a, the washing cavity 11b and the elution cavity 11c; the magnetic beads are built-in in the fixed part 11; the magnetic body 16 is built-in in the fixed part 11 and can magnetically attract the magnetic beads, the magnetic body 16 can drive the magnetic beads to transfer between the transfer channel 11d, the cracking cavity 11a, the washing cavity 11b and the elution cavity 11c, and the volume of the magnetic body 16 is greater than that of the magnetic beads. The magnetic beads are a kind of superparamagnetic microspheres with specific chemical functional groups on the surface, which are applied to the extraction and purification of DNA and RNA.

[0058] Specifically, when the full-closed nucleic acid extraction and detection device is in use, the purification mechanism 1 purifies the nucleic acid in the sample, the lysis cavity 11a is provided with a lysis solution, the sample enters the lysis cavity 11a, the sample is lysed by the lysis solution, the magnetic body 16 is driven to move by an external magnetic field or a special fluid driving mechanism, the magnetic body 16 magnetically adsorbs the magnetic beads and drives the magnetic beads to move, the magnetic beads are first moved into the lysis cavity 11a to adsorb the lysed nucleic acid; the magnetic beads are driven to move by the magnetic body 16 and enter the washing cavity 11b through the transfer channel 11d, the washing cavity 11b stores a washing solution, the washing solution can wash the nucleic acid adsorbed by the magnetic beads, and the washing solution is used to remove impurities such as proteins and salt ions that may be left on the magnetic beads. If these impurities remain in the subsequent reaction system, they may interfere with subsequent nucleic acid detection or amplification operations; the washed magnetic beads are driven to move by the magnetic body 16 and are transferred to the elution cavity 11c through the communication channel 11e, the elution cavity 11c stores an elution solution, the elution solution is used to elute the nucleic acid adsorbed on the magnetic beads, and the eluted nucleic acid enters the nucleic acid flow channel 1a, thereby realizing the purification of the nucleic acid in the sample.

[0059] The material of the magnetic body 16 can be iron, cobalt, nickel, silicon steel, permalloy, alnico, neodymium iron boron, samarium cobalt, ferrite, iron-based amorphous alloy, nanocrystalline alloy, etc. The shape of the magnetic body 16 can be spherical, shuttle-shaped, square-shaped, etc. The magnetic body 16 can be driven by the magnetic force rod of an existing detection instrument to move as described above, or can be driven by an external magnetic field or a special fluid driving mechanism to ensure that the magnetic beads can smoothly move along the transfer channel 11d.

[0060] The magnetic body 16 can be driven to move by cooperating with an external magnetic field to drive the magnetic beads to move, and the lysis, washing and elution are all performed in the fixed part 11 in a fully closed environment to avoid interference of external impurities with the purification of the nucleic acid. Moreover, the equipment does not need to enter the fixed part 11 during the purification of the nucleic acid, and there is no problem of nucleic acid leakage, which avoids nucleic acid leakage during detection and avoids interference of nucleic acid leakage with other nucleic acid detection. Moreover, if the magnetic beads are directly adsorbed and transferred by an external magnetic field, the magnetic beads are not easy to be completely adsorbed, and part of the magnetic beads will be left in the cavity during the transfer process, which avoids excessive loss of the magnetic beads and reduces the concentration of the nucleic acid. In the utility model, the magnetic body 16 is provided, the magnetic body 16 has magnetism and directly contacts the magnetic beads, can directly adsorb the magnetic beads, and avoids loss of the magnetic beads during the transfer process.

[0061] It should be understood that the washing cavity 11b is used to store a washing solution and waste liquid generated after washing, and the number of the washing cavities 11b is one or more than one; when the number of the washing cavities 11b is more than one, the washing solutions in the washing cavities 11b can be the same or different.

[0062] In order to control the movement of the magnetic body 16, as shown in one of the embodiments, Figure 12 the fully-enclosed nucleic acid extraction and detection device further comprises two magnetic attraction members 3 arranged outside the fixed part 11 and magnetically connected with the magnetic body 16.

[0063] The magnetic attraction members 3 can magnetically attract the magnetic body 16, and then move the magnetic attraction members 3 to drive the magnetic body 16 and the magnetic beads to move in the fixed part 11. It should be understood that the magnetic attraction members 3 can be permanent magnetic bodies or electromagnetic bodies, and can be manually controlled to move or controlled to move through a three-dimensional moving mechanism and a mechanical arm.

[0064] As shown in one of the embodiments, Figure 3 the fixed part 11 further forms a nucleic acid flow channel 1a; the fully-enclosed nucleic acid extraction and detection device further comprises a detection reagent mechanism 2, which is detachably connected with the fixed part 11 and forms a detection cavity 2a containing a detection reagent, one end of the detection cavity 2a being open; when the detection reagent mechanism 2 is connected with the fixed part 11, the detection cavity 2a is in communication with the nucleic acid flow channel 1a, and the purification mechanism 1 can block the opening of the detection cavity 2a. The number of the detection cavities 2a can be one, two or more.

[0065] The purified nucleic acid enters the nucleic acid flow channel 1a, and the nucleic acid flow channel 1a is closed during the purification process. When the purification is completed, the nucleic acid flow channel 1a is opened, and the nucleic acid enters the detection cavity 2a through the nucleic acid flow channel 1a, mixes and dissolves with the detection reagent in the detection cavity 2a, and then is amplified. Then, whether the amplified nucleic acid produces a fluorescence reaction is detected through a fluorescence detection device, so as to perform detection.

[0066] The purification mechanism 1 forms a nucleic acid flow channel 1a that can be opened and closed; the detection reagent mechanism 2 is detachably connected with the purification mechanism 1 and forms a detection cavity 2a containing a detection reagent, one end of the detection cavity 2a being open; when the detection reagent mechanism 2 is connected with the purification mechanism 1, the detection cavity 2a is in communication with the nucleic acid flow channel 1a, and the purification mechanism 1 can block the opening of the detection cavity 2a. It should be understood that the detection reagent mechanism 2 can also be directly fixedly connected with the purification mechanism 1 as a whole after pre-preparing the reagent, and the fixed connection can be welding or bonding.

[0067] Since the detection reagent mechanism 2 is detachably connected with the purification mechanism 1, different detection reagents can be filled into the detection cavity 2a for different samples to be detected, and the user can select reagents from different suppliers according to different samples to be detected, and then connect the detection reagent mechanism 2 with the purification mechanism 1. After connection, the reagent filled in the detection reagent mechanism 2 can react with the purified nucleic acid, and different targets of the nucleic acid can be detected according to different reagents.

[0068] After the detection reagent mechanism 2 is connected with the purification mechanism 1, the purification mechanism 1 can seal the opening of the detection cavity 2a, so as to avoid leakage of the nucleic acid during detection and interference of the nucleic acid leakage with other nucleic acid detection.

[0069] It should be understood that the purification mechanism 1 and the detection reagent mechanism 2 can be detachably connected by screws, screws, buckles and the like, or can be detachably connected by tight insertion.

[0070] In order to realize the detachable connection between the purification mechanism 1 and the detection reagent mechanism 2, as shown in Figure 1 one embodiment, the purification mechanism 1 and the detection reagent mechanism 2 are detachably connected by an insertion structure, and the insertion structure can seal the opening of the detection cavity 2a, and the detection cavity 2a can be pressurized when the insertion structure is inserted into the detection cavity 2a.

[0071] In this embodiment, the detachable connection between the purification mechanism 1 and the detection reagent mechanism 2 is realized by insertion between the purification mechanism 1 and the detection reagent mechanism 2, and the opening of the detection cavity 2a is sealed by the insertion between the purification mechanism 1 and the detection reagent mechanism 2. At the same time, when the purification mechanism 1 and the detection reagent mechanism 2 are inserted, the nucleic acid flow channel 1a is closed, and part of the insertion structure is inserted into the detection cavity 2a, which can extrude the space in the detection cavity 2a and compress the gas in the detection cavity 2a, so that the gas in the detection cavity 2a is pressurized. When the fully-closed nucleic acid extraction and detection device is applied to a plateau environment, the boiling point of the solution in the detection cavity 2a can be raised due to the pressurization of the gas in the detection cavity 2a, so as to avoid premature boiling of the solution and loss of the reagent caused by boiling of the solution, and facilitate heating of the reagent in the detection cavity 2a.

[0072] The sealing of the opening means that the reagent is prevented from flowing out from the gap between the purification mechanism 1 and the detection reagent mechanism 2, but the nucleic acid is not limited to entering the detection cavity 2a from the nucleic acid flow channel 1a.

[0073] When the nucleic acid in the detection cavity 2a is detected by the detection device, the solution in the detection cavity 2a will be heated. Since the amount of the solution in the detection cavity 2a is small, the solution in the detection cavity 2a will evaporate during the heating process, which will reduce the amount of the solution in the detection cavity 2a and affect the detection of the nucleic acid. To this end, as shown in Figure 9 and Figure 10 In one of the embodiments, the purification mechanism 1 includes a fixed part 11, a plug-in part 12, and a paraffin layer 13. The fixed part 11 forms the nucleic acid flow channel 1a and is detachably connected with the detection reagent mechanism 2. The plug-in part 12 is arranged opposite to the detection cavity 2a and is connected with the fixed part 11. The paraffin layer 13 is connected with the plug-in part 12. When the detection reagent mechanism 2 is connected with the purification mechanism 1, the plug-in part 12 and the paraffin layer 13 are inserted into the detection cavity 2a. It should be understood that the paraffin layer 13 can also be other substances that can play a similar role.

[0074] In the embodiment, the fixed part 11 has the plug-in part 12 extending into the detection cavity 2a, and the plug-in part 12 is provided with the paraffin layer 13. When the nucleic acid is detected, the solution in the detection cavity 2a will be heated, which will cause the paraffin layer 13 to melt. Since the density of paraffin is less than that of water, the melted paraffin layer 13 will float on the top of the detection reagent, which will block the evaporation of the water and the detection reagent in the detection cavity 2a, and avoid the reduction of the detection reagent caused by the evaporation of the water and the detection reagent.

[0075] It should be understood that the elution cavity 11c can be completely sealed in the fixed part 11, or can be open on one side. When the elution cavity 11c can be completely sealed in the fixed part 11, the transfer channel 11d can directly communicate with the elution cavity 11c. To this end, in one of the embodiments, the elution cavity 11c is open on one side, and the fixed part 11 further forms a communication channel 11e. The transfer channel 11d communicates with the elution cavity 11c through the communication channel 11e.

[0076] Since the elution cavity 11c is open on one side, the inner wall of the open side will hinder the movement of the magnetic beads and the magnetic body 16. To this end, in the embodiment, the communication channel 11e is provided, which is used for the magnetic beads and the magnetic body 16 to enter the elution cavity 11c from the transfer channel 11d.

[0077] To avoid the leakage of the reagents sealed in the lysis cavity 11a, the washing cavity 11b, and the elution cavity 11c, as shown in Figure 6 In one of the embodiments, the purification mechanism 1 further includes a sealing layer 14. The sealing layer 14 is arranged in the transfer channel 11d and is detachably connected with the fixed part 11. When the sealing layer 14 is connected with the fixed part 11, it blocks the communication between the lysis cavity 11a, the washing cavity 11b, and the transfer channel 11d, and blocks the communication between the communication channel 11e and the transfer channel 11d.

[0078] In this embodiment, by providing a sealing layer 14, the sealing layer 14 can seal the pyrolysis chamber 11a, washing chamber 11b, and elution chamber 11c before using the purification mechanism 1, preventing the reagents in the pyrolysis chamber 11a, washing chamber 11b, and elution chamber 11c from overflowing and mixing. When testing is required, the sealing layer 14 can be removed from the fixing part 11 so that the magnetic beads can enter the pyrolysis chamber 11a, washing chamber 11b, and elution chamber 11c.

[0079] The sealing layer 14 can be a thin film that is connected to the fixing part 11 by means of adhesive bonding or the like, or it can be a sealing sheet that is sealed by a sealing ring and connected to the fixing part 11. The sealing sheet and the fixing part 11 can be detachably connected by means of snap-fit ​​or the like.

[0080] In order to detach the sealing layer 14 from the sealing position, for this purpose, as follows: Figure 3 , 4 As shown in Figure 6, in one embodiment, the fixing part 11 has a mounting cavity 11f that communicates with the transfer channel 11d; the purification mechanism 1 also includes a winding part 15, which is rotatably disposed in the mounting cavity 11f and connected to the sealing layer 14. The winding part 15 is rotatably disposed in the mounting cavity 11f and connected to the sealing layer 14 to wind the sealing layer 14 to release the blockage at the connection between the pyrolysis cavity 11a, the washing cavity 11b and the transfer channel 11d, and to release the blockage at the connection between the connecting channel 11e and the transfer channel 11d.

[0081] In this embodiment, when a fully enclosed nucleic acid extraction and detection device is required, the winding part 15 is rotated. The winding part 15 rotates and continuously winds the sealing layer 14, so that the sealing layer 14 is removed from its original sealed position, thereby releasing the blockage at the connection between the lysis chamber 11a, the washing chamber 11b and the transfer channel 11d, and releasing the blockage at the connection between the connecting channel 11e and the transfer channel 11d.

[0082] It should be understood that the sealing layer 14 can be sealed by heat sealing or ultrasonic welding using a sealing plastic film or aluminum film.

[0083] It should be understood that the winding portion 15 can be built into the mounting cavity 11f or extend out of the mounting cavity 11f. When the winding portion 15 is built into the mounting cavity 11f, it can be connected to an external drive motor by magnetic connection so that the winding portion 15 can be driven to rotate by the external drive motor. When the winding portion 15 extends out of the mounting cavity 11f, the winding portion 15 and the fixing portion 11 can be sealed by a sealing ring or by fitting. The winding portion 15 can be rotated by manual drive or by drive equipment such as a motor. The winding portion 15 and the sealing layer 14 can be connected by bonding, insertion, or snap-fit.

[0084] In order to peel the sealing layer 14 from the blocked position, the sealing layer 14 can be directly pulled, but directly pulling the end of the sealing film is easy to cause the sealing layer 14 to break and cannot guarantee that the sealing layer 14 is completely separated. Therefore, as shown in one of the embodiments, a window 14a is provided in the middle of the sealing layer 14, the size of the two ends of the sealing layer 14 is at least partially smaller than the size of the window 14a, and the two ends of the sealing layer 14 pass through the window 14a and are connected to the winding part 15. Figure 6

[0085] In the embodiment, the two ends of the sealing layer 14 are not connected to the fixed part 11, and the two ends of the sealing layer 14 are wound by the winding part 15, so that the sealing layer 14 can be torn from the end to the center, and in the tearing process, the sealing layer 14 is pulled away from the fixed position, which facilitates tearing the sealing layer 14.

[0086] In order to drive the magnetic beads to transfer between the transfer channel 11d, the lysis cavity 11a, the washing cavity 11b, the communication channel 11e, and the elution cavity 11c, the magnetic beads can be directly driven to move along the transfer channel 11d by an external magnetic field, but it is not easy to drive all the magnetic beads to transfer during the transfer, and secondly, since the size of the magnetic beads is small, it is not possible to determine whether the magnetic beads have been transferred. Therefore, as shown in one of the embodiments, the purification mechanism 1 further comprises a magnetic body 16, the magnetic body 16 is built in the fixed part 11, the magnetic body 16 can magnetically attract the magnetic beads and can be transferred between the transfer channel 11d, the lysis cavity 11a, the washing cavity 11b, the communication channel 11e, and the elution cavity 11c, and the volume of the magnetic body 16 is greater than that of the magnetic beads. Figure 5

[0087] In the embodiment, by providing the magnetic body 16, after the magnetic beads adsorb nucleic acids, the magnetic body 16 is driven to move to the adsorbed magnetic beads by an external magnetic field or a special fluid driving mechanism, and then the magnetic beads are driven to pass through the lysis cavity 11a, the washing cavity 11b, and enter the elution cavity 11c in turn, and the magnetic beads are adsorbed by the magnetic body 16 to facilitate the transfer of the magnetic beads between the lysis cavity 11a, the washing cavity 11b, and the elution cavity 11c. At the same time, by providing the magnetic body 16, and since the volume of the magnetic beads is small and the volume of the magnetic body 16 is greater than that of the magnetic beads, the position of the magnetic body 16 can be observed to facilitate the determination of whether the magnetic beads have moved to the set location.

[0088] It should be understood that the magnetic body 16 can be various types of permanent magnetic bodies or electromagnetic bodies, and specifically, as shown in one of the embodiments, the magnetic body 16 is a spherical permanent magnetic body. Figure 5

[0089] It should be understood that the lysis cavity 11a, the washing cavity 11b, and the elution cavity 11c can be arranged in a matrix or in a linear arrangement, and specifically, as shown in one of the embodiments, the lysis cavity 11a, the washing cavity 11b, and the elution cavity 11c are arranged in a linear arrangement.​​​Figure 3 、 4 In one of the embodiments shown in FIG. 5, the lysis cavity 11a, the washing cavity 11b and the elution cavity 11c are linearly arranged along the first direction, and the side walls on both sides of the lysis cavity 11a, the washing cavity 11b and the elution cavity 11c along the first direction are smoothly connected to the inner walls of the cavities in other directions by arc surfaces.

[0090] In the embodiment, the lysis cavity 11a, the washing cavity 11b and the elution cavity 11c are linearly arranged along the first direction, so that the side walls on both sides of the lysis cavity 11a, the washing cavity 11b and the elution cavity 11c along the first direction are close to the outer wall of the fixed part 11, avoiding the overlapping of the cavities along the direction perpendicular to the first direction. In order to stir the liquid in the cavities, make the magnetic beads fully adsorb the nucleic acid, fully wash the nucleic acid and fully elute the nucleic acid, in the embodiment, a first magnetic field is arranged on one side of the fixed part 11 along the first direction, at this time, the first magnetic field is magnetically adsorbed with the magnetic body 16 and the magnetic beads, so that the magnetic body 16 and the magnetic beads move to the inner wall of one side of the cavity, then a second magnetic field is arranged on the other side of the fixed part 11 along the first direction, the first magnetic field is controlled to move along the second direction perpendicular to the first direction, the first magnetic field drives the magnetic body 16 and the magnetic beads to move along the first direction, until the magnetic body 16 and the magnetic beads move to the edge position of the inner wall of one side of the cavity, at this time, the magnetic body 16 and the magnetic beads cannot move along the first direction due to the limitation of the inner wall of the cavity, when the first magnetic field moves far enough to make the magnetic force of the first magnetic field applied to the magnetic body 16 and the magnetic beads disappear, the magnetic body 16 and the magnetic beads are adsorbed to the inner wall close to the other side of the cavity under the magnetic adsorption of the second magnetic field, and in the moving process of the magnetic body 16 and the magnetic beads, the magnetic body 16 drives the magnetic beads to hit the smooth surface of the cavity and slide along the arc surface, so that the magnetic body 16 and the magnetic beads move in an arc shape in the cavity, and repeating the above steps can stir the solution in the cavity.

[0091] The first direction can be any direction, and in one of the embodiments, the first direction is the length direction of the fixed part 11.

[0092] It should be understood that the first magnetic field and the second magnetic field can be formed by permanent magnetic bodies or electromagnetic bodies, and in one of the embodiments, the first magnetic field and the second magnetic field are two magnetic members 3 arranged on both sides of the fixed part 11 along the first direction.

[0093] The movement of the first magnetic field and the second magnetic field can be manual movement, or the movement of the first magnetic field and the second magnetic field can be controlled by a moving device; two permanent magnetic bodies can be arranged on the side walls on both sides of the fixed part 11 along the first direction to control the formation of the magnetic field, and the magnetic field is moved by controlling the movement of the permanent magnetic bodies.

[0094] It should be understood that the opening and closing of the nucleic acid flow channel 1a can be achieved through a valve or other methods, specifically, such as... Figure 3 As shown, in one embodiment, the nucleic acid flow channel 1a has a sealed cavity 1a1. The purification mechanism 1 includes a sealing part 17 and an elution plug 18. The sealing part 17 is built into the sealed cavity 1a1 and seals against the inner wall of the sealed cavity 1a1 to close the nucleic acid flow channel 1a. The elution plug 18 is slidably disposed in the sealed cavity 1a1. The elution plug 18 is used to push the sealing part 17 out of the sealed cavity 1a1 by the fluid in the elution cavity 11c to open the nucleic acid flow channel 1a. The sealing part 17 can be a silicone ball, a rubber ball, a rubber block, etc.

[0095] In this embodiment, during the purification of nucleic acid, the elution plug 18 remains stationary. When the magnetic chuck 16 moves the magnetic bead into the elution chamber 11c and performs elution, the nucleic acid flow needs to be activated so that the eluted nucleic acid in the elution chamber 11c can enter the nucleic acid flow channel 1a and then enter the detection chamber 2a. At this time, the elution plug 18 is deactivated, and the elution plug 18 gradually closes the connection between the elution chamber 11c and the connecting channel 11e. The elution plug 18 continues to slide, pressurizing the flow in the elution chamber 11c. The pressurized flow pushes the sealing part 17 in the sealing chamber 1a1 to slide, causing the sealing part 17 to detach from the sealing chamber 1a1. At this time, the nucleic acid flow channel 1a opens, and the fluid in the elution chamber 11c can flow through the nucleic acid flow channel 1a and enter each detection chamber 2a.

[0096] It should be understood that, in order to seal and open the liquid inlet channel 111e, in one embodiment, the liquid inlet channel 111e also has a sealing cavity 1a1, and the sealing cavity 1a1 is also provided with a sealing part 17 that can be engaged and disengaged.

[0097] It should be understood that in this application, the purification mechanism 1 and the fixing part 11 can both be integrally formed structures, or they can be formed by combining and splicing multiple components. During the splicing process, welding, bonding, bolts, screws and buckles can be used to achieve this.

[0098] Specifically, such as Figure 3 , 4As shown in Figure 5, in one embodiment, the fixing part 11 includes a first housing 111, a second housing 112, and a third housing 113 connected in sequence. The first housing 111 has a first through hole 111a, and the second housing 112 has a first fixing hole 112a opposite to the first through hole 111a. The first fixing hole 112a and the first through hole 111a combine to form an elution chamber 11c. The second housing 112 also has a pyrolysis chamber 11a, at least one washing chamber 11b, a mounting chamber 11f, a connecting channel 11e, and a sealing chamber 11a1. A transfer channel 11d is formed between the second housing 112 and the first housing 111. The pyrolysis chamber 11a and the washing chamber 11b... b. The mounting cavity 11f opens to the side facing the first housing 111 and is connected to the transfer channel 11d through the opening. The sealing cavity 1a1 is connected to the first fixing hole 112a and is located on the side of the first fixing hole 112a away from the first housing 111. One end of the connecting channel 11e is connected to the transfer channel 11d and the other end is connected to the first fixing hole 112a. The third housing 113 and the second housing 112 are combined to form a connecting groove 1a2. The third housing 113 has a connecting hole 1a3 that is connected to the connecting groove 1a2 and the detection cavity 2a. The sealing cavity 1a1, the connecting groove 1a2 and the connecting hole 1a3 are connected and combined to form a nucleic acid flow channel 1a.

[0099] During operation, the extracted sample is introduced into the lysis chamber 11a, and magnetic beads are introduced into the lysis chamber 11a. The lysed nucleic acid is adsorbed by the magnetic beads, and then the magnetic beads are driven by the magnetic suction body 16 to pass through the lysis chamber 11a, the transfer channel 11d, the washing chamber 11b, the transfer channel 11d, the connecting channel 11e, and the elution chamber 11c in sequence. After passing through the elution chamber 11c, the sample enters the sealing chamber 1a1. Under the push of the elution plug 18, the sample enters the connecting groove 1a2, and then enters the connecting hole 1a3 through the connecting groove 1a2, and finally enters the detection chamber 2a.

[0100] like Figure 3 As shown, a relatively large chamber is provided at the connection between the connecting groove 1a2 and the sealing cavity 1a1. This chamber accommodates the squeezed-down sealing part 17, so that there is a space between the third housing 113 and the second housing 112 to accommodate the sealed part 17 after the seal is released, and liquid can flow through the squeezed sealing part 17.

[0101] Since the first fixing hole 112a and the first through hole 111a combine to form the elution chamber 11c, the obstruction of the inner wall of the first through hole 111a prevents the transfer channel 11d from directly connecting to the first fixing hole 112a, thus preventing the magnetic attractor 16 from driving the magnetic bead into the first fixing hole 112a. Therefore, as follows: Figure 3 and Figure 5As shown, in one of the embodiments, the end surface of the top inner wall of the communication channel 11e is flush with the end surface of the top inner wall of the washing cavity 11b, the communication channel 11e is arc-shaped, and the communication part of the communication channel 11e with the first fixed hole 112a is located on the peripheral wall of the first fixed hole 112a.

[0102] In the present embodiment, by setting the communication channel 11e to be arc-shaped and setting the communication part of the communication channel 11e with the first fixed hole 112a on the peripheral wall of the first fixed hole 112a, when the magnetic attraction body 16 drives the magnetic beads to move in the transfer channel 11d, the magnetic beads can enter the communication channel 11e from the transfer channel 11d, slide in the communication channel 11e in an arc shape, and slide into the second fixed hole 112b, thereby realizing the transfer of nucleic acid into the elution cavity 11c.

[0103] In order to realize the entry of the sample into the lysis cavity 11a, for this purpose, as shown in FIG. 1, the first shell 111 is provided with a second through hole 111b, and the second shell 112 is provided with a second fixed hole 112b opposite to the second through hole 111b. Figure 3 、 4 In one of the embodiments, the first shell 111 is provided with a second through hole 111b, the second shell 112 is provided with a second fixed hole 112b opposite to the second through hole 111b, a puncture part 114 is formed in the second fixed hole 112b, and the second fixed hole 112b and the second through hole 111b combine to form a sampling cavity. The side of the second shell 112 away from the first shell 111 is also provided with a liquid inlet channel 111e communicating with the lysis cavity 11a, the communication part of the liquid inlet channel 111e with the lysis cavity 11a is higher than the bottom inner wall of the lysis cavity 11a, and the purification mechanism 1 further comprises a sampling member 19, which comprises a sample tube 191, a sampling plug 192, and a sealing plug 193. The sample tube 191 is slidingly inserted into the sampling cavity, the sampling plug 192 is cooperatively and slidingly inserted into the sample tube 191, and the sealing plug 193 is cooperatively inserted into the second through hole 111b and used to seal the open end of the sampling cavity.

[0104] The sample is stored in the sample tube 191, and a preservative liquid is arranged in the sample tube 191 to prevent the tissue sample, blood, saliva and the like from deteriorating. When the sample in the sample tube 191 needs to be sent into the lysis cavity 11a, the closure plug 193 is opened, the sample tube 191 is inserted into the sampling cavity, and the bottom of the sample tube 191 abuts against the puncture portion 114. The bottom of the sample tube 191 is opened by puncture of the puncture portion 114, and then the sampling plug 192 is slid to press the sample in the sample tube 191 into the sampling cavity. The sample enters the lysis cavity 11a through the liquid inlet channel 111e at the bottom of the sampling cavity, and then the opening of the sampling cavity is closed by the closure plug 193. Since the communication portion of the liquid inlet channel 111e and the lysis cavity 11a is higher than the inner wall of the bottom of the lysis cavity 11a, the magnetic beads can be prevented from entering the liquid inlet channel 111e. The sampling plug 192 can be slid by manual control or relative to the sample tube 191 under the drive of the motor rod. The liquid in the sample tube 191 pushes away the elastic sealing portion 17 at the bottom to enter the lysis cavity 11a through the flow channel at the bottom.

[0105] In order to store the magnetic beads and the magnetic attraction body 16, in one embodiment, the second shell 112 is further provided with a first storage hole 112c and a second storage hole 112d. The first storage hole 112c and the second storage hole 112d are open to one side of the first shell 111 and are in communication with the transfer channel 11d. The top end faces of the first storage hole 112c, the second storage hole 112d, the lysis cavity 11a, the washing cavity 11b and the communication channel 11e are flush. The sealing layer 14 is arranged on the top of the first storage hole 112c, the second storage hole 112d, the lysis cavity 11a, the washing cavity 11b, the communication channel 11e and the sealing cavity 1a1. The magnetic beads are arranged in the first storage hole 112c, and the magnetic attraction body 16 is arranged in the second storage hole 112d.

[0106] In the embodiment, the first storage hole 112c and the second storage hole 112d can store the magnetic beads and the magnetic attraction body 16, respectively. Before use, the product can be fixed in the first storage hole 112c and the second storage hole 112d, respectively, to prevent the magnetic beads and the magnetic attraction body 16 from being scattered between the first shell 111 and the second shell 112. By making the top end faces of the first storage hole 112c, the second storage hole 112d, the lysis cavity 11a, the washing cavity 11b and the communication channel 11e flush, the first storage hole 112c, the second storage hole 112d, the lysis cavity 11a, the washing cavity 11b and the communication channel 11e can be closed during the process of attaching the sealing layer 14 to the top end faces of the first storage hole 112c, the second storage hole 112d, the lysis cavity 11a, the washing cavity 11b and the communication channel 11e.

[0107] In order to realize the sealing between the purification mechanism 1 and the detection reagent mechanism 2, as shown in Figure 8 And Figure 10 In one embodiment, the plug-in part 12 includes a fixed ring 121, a plug-in block 122 and a plug-in strip 123, the fixed ring 121 is connected to the side of the third shell 113 away from the second shell 112, one end of the plug-in block 122 is connected to the third shell 113 and passes through the fixed ring 121, the communication hole 1a3 penetrates through the plug-in block 122, the plug-in strip 123 is connected to the plug-in block 122 and is arranged on the side of the plug-in block 122 away from the third shell 113; the detection reagent mechanism 2 includes a fourth shell 21 and a detection shell 22 connected to the fourth shell 21, the detection shell 22 is connected to the fourth shell 21, the outer diameter of the detection shell 22 matches the inner diameter of the fixed ring 121, the detection shell 22 is formed with a detection cavity 2a, the top of the detection cavity 2a is open, the detection cavity 2a can be inserted into the plug-in block 122 and the plug-in strip 123, and the plug-in strip 123 is provided with a paraffin layer 13.

[0108] In this embodiment, in order to realize the sealing between the detection reagent mechanism 2 and the purification mechanism 1, the detection shell 22 is matched with the fixed ring 121, the sealing connection of the two is realized through the sealing matching of the detection shell 22 and the fixed ring 121, the detection cavity 2a is used for storing reagents, and at the same time, the plug-in block 122 and the plug-in strip 123 can be inserted.

[0109] It should be understood that the detection shell 22 and the plug-in part 12 can be one, two and multiple, and specifically, the detection shell 22 and the plug-in part 12 are multiple, the multiple detection shells 22 are distributed along the length direction of the fourth shell 21, the plug-in part 12 is arranged in one-to-one correspondence with the detection shell 22, and the communication hole 1a3 in the plug-in block 122 in the multiple plug-in parts 12 is in communication with the communication groove 1a2.

[0110] Among them, the central axes of the multiple plug-in parts 12 and the detection shell 22 only need to be parallel to each other, and the rotation angle of the plug-in part 12 and the detection shell 22 along the central axis can be set according to needs, which can be 90°, 180°, etc. It should be understood that the outer contour of the detection shell 22 can be in the form of a flat tube, or in the form of a flat tube, a cone, a cylinder or other polygonal body.

[0111] In the full-closed nucleic acid extraction and detection device, the nucleic acid is sealed in the device during the detection process, which can avoid the leakage of the nucleic acid outward to contaminate other samples and interfere with other detections, the nucleic acid detection does not need to be performed in a standard molecular diagnostic laboratory, and the nucleic acid extraction and amplification process can be shortened, the influence of human factors can be reduced, and the safety and effectiveness of nucleic acid sample preparation can be enhanced.

[0112] In the description of the utility model, it is necessary to understand that the orientation or positional relation indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like is the orientation or positional relation shown based on the drawings, and is only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model.

[0113] The terms "first", "second", "third", etc. are used only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated.

[0114] In the description of the utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connecting" should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrally connected, it can be directly connected, or indirectly connected through an intermediate medium, it can be the communication inside two elements.

[0115] In the description of the specification, specific features, structures, materials or characteristics can be combined in any one or more embodiments or examples in a suitable manner.

[0116] The specific embodiments of the utility model described above do not constitute a limitation on the protection scope of the utility model. Any various other corresponding changes and modifications made according to the technical concept of the utility model should be included in the protection scope of the claims of the utility model.

Claims

1. A totally enclosed nucleic acid extraction and detection device, characterized by, The purification mechanism (1) comprises: a fixed part (11) having a lysis cavity (11a), at least one washing cavity (11b), an elution cavity (11c), and a transfer channel (11d) connecting the lysis cavity (11a), the washing cavity (11b), and the elution cavity (11c); magnetic beads arranged in the fixed part (11); and a magnetic body (16) arranged in the fixed part (11) and capable of magnetically attracting the magnetic beads, the magnetic body (16) being capable of driving the magnetic beads to transfer between the transfer channel (11d), the lysis cavity (11a), the washing cavity (11b), and the elution cavity (11c), and the volume of the magnetic body (16) being greater than that of the magnetic beads.

2. The fully-closed nucleic acid extraction and detection device according to claim 1, further comprising two magnetic members (3) arranged outside the fixed part (11) and magnetically connected with the magnetic body (16).

3. The fully-closed nucleic acid extraction and detection device according to claim 1, wherein the fixed part (11) further has a nucleic acid flow channel (1a); and the fully-closed nucleic acid extraction and detection device further comprises a detection reagent mechanism (2) detachably connected with the fixed part (11) and having a detection cavity (2a) containing a detection reagent, one end of the detection cavity (2a) being open; when the detection reagent mechanism (2) is connected with the fixed part (11), the detection cavity (2a) is connected with the nucleic acid flow channel (1a), and the purification mechanism (1) can block the opening of the detection cavity (2a).

4. The fully-closed nucleic acid extraction and detection device according to claim 3, wherein the purification mechanism (1) and the detection reagent mechanism (2) are detachably connected by a plug-in structure capable of sealing the opening of the detection cavity (2a), and when the purification mechanism (1) is plugged into the detection reagent mechanism (2), the plug-in structure can at least partially insert into the detection cavity (2a) and pressurize the detection cavity (2a).

5. The fully-closed nucleic acid extraction and detection device according to claim 3, wherein the purification mechanism (1) further comprises: a plug-in part (12) connected with the fixed part (11); and a paraffin layer (13) connected with the plug-in part (12); when the detection reagent mechanism (2) is connected with the purification mechanism (1), the plug-in part (12) and the paraffin layer (13) are arranged in the detection cavity (2a).

6. The fully-closed nucleic acid extraction and detection device according to claim 1, wherein the purification mechanism (1) further comprises: ​ ​ ​ ​ ​ ​ ​ ​ A sealing layer (14) is arranged in the transfer channel (11d) and detachably connected with the fixed part (11), and the sealing layer (14) blocks the communication between the lysis chamber (11a), the washing chamber (11b) and the transfer channel (11d) when connected with the fixed part (11).

7. The fully-closed nucleic acid extraction and detection device according to claim 6, wherein An installation chamber (11f) is formed in the fixed part (11) and communicates with the transfer channel (11d); The purification mechanism (1) further comprises: A winding part (15) is rotatably arranged in the installation chamber (11f) and connected with the sealing layer (14), and is used for winding the sealing layer (14) to unblock the communication between the lysis chamber (11a), the washing chamber (11b) and the elution chamber (11c) and the transfer channel (11d).

8. The fully-closed nucleic acid extraction and detection device according to claim 7, wherein A window (14a) is formed in the middle of the sealing layer (14), the size of the two ends of the sealing layer (14) is at least partially smaller than the size of the window (14a), and the two ends of the sealing layer (14) pass through the window (14a) and are connected with the winding part (15).

9. The fully-closed nucleic acid extraction and detection device according to claim 1, wherein The lysis chamber (11a), the washing chamber (11b) and the elution chamber (11c) are linearly arranged along a first direction, and the side walls on both sides of at least some of the chambers along the first direction are smoothly connected with the inner walls of the chambers in other directions through a circular arc surface.

10. The fully-closed nucleic acid extraction and detection device according to claim 3, wherein The nucleic acid flow channel (1a) has a sealing chamber (1a1); The purification mechanism (1) comprises: A sealing part (17) is arranged in the sealing chamber (1a1) and sealingly cooperates with the inner wall of the sealing chamber (1a1) to close the nucleic acid flow channel (1a); and An elution plug (18) is slidingly arranged in the sealing chamber (1a1), and the elution plug (18) is used to push the sealing part (17) out of the sealing chamber (1a1) by the fluid in the elution chamber (11c) to open the nucleic acid flow channel (1a).

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