Efficient pretreatment device for gene sequencing sample

Abstract

The utility model relates to the technical field of gene sequencing, and discloses a gene sequencing sample efficient pretreatment device which comprises an installation box, a base is fixedly connected to the left side of the inner bottom wall of the installation box, a treatment tank is arranged at the top of the base, a motor is installed at the bottom of the treatment tank, and the output end of the motor is fixedly connected with a rotating rod. And scraping plates are fixedly connected to the outer side of the rotating rod at equal intervals, a guide pipe communicates with the middle of the top end of the treatment tank, and a cleaning mechanism is mounted at the top of the mounting box and used for cleaning the interior of the mounting box. According to the utility model, firstly, the motor is started, the motor drives the rotating rod to rotate, and the rotating rod rotates to drive the scraping plate to scrape the inner wall of the treatment tank, so that samples can be uniformly mixed, residues can be prevented, the samples are input and output through the guide pipe, efficient pretreatment is realized, and the data accuracy can be improved through the procedures.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of gene sequencing technology especially relates to gene sequencing sample efficient pretreatment device. BACKGROUND

[0002] The gene sequencing sample is the biological material obtained from a living body and used for gene sequencing analysis, and common types include blood samples, tissue samples, saliva samples, amniotic fluid samples and villus samples.

[0003] The gene sequencing sample efficient pretreatment device is used for the preliminary treatment of gene sequencing samples to improve the processing efficiency and quality. Related equipment of Shenzhen Huada Gene Research Institute includes a placement platform, a driving device, a pipette and a temperature control device, which can realize liquid transfer, sample purification and incubation functions. Some devices can integrate the cell liquid crushing, washing and separation processes, such as a device with a crushing, washing and separation device, multiple storage devices and an ultrasonic generator. There are also devices that can separate and stratify the DNA extraction liquid by setting a filtering mechanism and a stratification mechanism, as well as gene sequencing instrument sample pretreatment structures that can crush, wash and separate samples and are convenient to clean and maintain. However, during use, the internal structure of some devices is complex, with many hard-to-reach corners and gaps. After the sample processing is completed, these parts may leave samples or reagents, which may breed microorganisms or cause cross-contamination to subsequent samples. The cleaning process is tedious and requires a lot of time and effort. In the prior art, the internal surface of the device is designed to be as smooth as possible to reduce unevenness and hard-to-reach corners. For example, the corners are designed as rounded corners, and the pipeline connection uses smooth transition to reduce the possibility of sample and dirt residue. However, during use, it is impossible to completely avoid sample adhesion on the inner wall of the device. Residual samples can affect the results of the next detection, leading to inaccurate data or false positive and false negative problems. SUMMARY

[0004] To make up for the above shortcomings, the gene sequencing sample efficient pretreatment device is provided to improve the problem that in the prior art, sample adhesion on the inner wall of the device cannot be completely avoided, residual samples can affect the results of the next detection, leading to inaccurate data or false positive and false negative problems.

[0005] In order to achieve the above object, the utility model discloses the following technical scheme: Gene sequencing sample high -efficient pretreatment device, including installation box, the inner bottom wall left side fixedly connected with the base of installation box, the top of base is provided with processing jar, the bottom of processing jar is installed with motor, the output fixedly connected with rotating rod of motor, the equal interval fixedly connected with scraper of rotating rod's outside, the top department of processing jar is communicated with the pipe, the top of installation box is installed with cleaning mechanism, and the cleaning mechanism is used for the cleaning of installation box inside.

[0006] As further description of the above technical scheme:

[0007] The cleaning mechanism includes a fixed plate, the fixed plate is rotatably connected to the middle of the top of the installation box, a stirring box is installed on the back side of the fixed plate, a rotating shaft is rotatably connected to the middle of the stirring box, a plurality of stirring blades are fixedly connected to the outer side of the rotating shaft, a feed pipe is communicated to the left end of the back side of the stirring box, and a plurality of nozzles are fixedly connected to the front side of the fixed plate.

[0008] As further description of the above technical scheme:

[0009] The right side of the rotating shaft penetrates the right side of the stirring box and is fixedly connected with a rotating disc, and the right top of the rotating disc is fixedly connected with a fixed stub.

[0010] As further description of the above technical scheme:

[0011] A handle is installed on the middle of the back side of the stirring box, and screws are threadedly connected to the left and right ends of the handle.

[0012] As further description of the above technical scheme:

[0013] A circular cover is installed on the back side of the feed pipe, and a mounting stub is fixedly connected to the middle of the back side of the circular cover.

[0014] As further description of the above technical scheme:

[0015] A clamping groove plate is fixedly connected to the middle of the right side of the installation box, and a notice board is installed in the inside of the clamping groove plate.

[0016] As further description of the above technical scheme:

[0017] A transparent plate is installed on the middle of the front side of the installation box.

[0018] As further description of the above technical scheme:

[0019] An installation plate is fixedly connected to the middle of the left side of the installation box, and a plurality of hooks are fixedly connected to the left side of the installation plate.

[0020] The utility model has the advantages of the following beneficial effects:

[0021] 1. In the utility model, first, the motor is started, the motor drives the rotating rod to rotate, the rotation of the rotating rod drives the scraper to scrape on the inner wall of the processing tank, ensures uniform mixing of the sample and prevents residue, the input and output of the sample are realized through the conduit, efficient pretreatment is realized, and the data accuracy is improved.

[0022] 2. In the utility model, the cleaning liquid is conveyed to the stirring box through the feed pipe, the rotating shaft drives the stirring blade to rotate, stirring and mixing are realized, the best cleaning effect is achieved, after stirring is completed, the fixed plate is rotated, the stirring box is rotated to a suitable position, subsequent operation is facilitated, the mixed cleaning liquid is sprayed out through the nozzle, uniformly covers the inside of the mounting box, and overall cleaning is realized. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 A perspective view of the gene sequencing sample efficient pretreatment device is provided for the utility model;

[0024] Figure 2 A front view of the gene sequencing sample efficient pretreatment device is provided for the utility model;

[0025] Figure 3 A side view of the gene sequencing sample efficient pretreatment device is provided for the utility model;

[0026] Figure 4 A partial structure split view of the gene sequencing sample efficient pretreatment device is provided for the utility model;

[0027] Figure 5 A partial structure schematic view of the gene sequencing sample efficient pretreatment device is provided for the utility model.

[0028] LEGEND:

[0029] 1, mounting box;2, cleaning mechanism;201, stirring box;202, feed pipe;203, nozzle;204, rotating shaft;205, stirring blade;206, fixed plate;3, rotating disc;4, fixed stub;5, signboard;6, clamping groove plate;7, transparent plate;8, mounting plate;9, hook;10, handle;11, screw;12, processing tank;13, circular cover;14, mounting stub;15, rotating rod;16, scraper;17, conduit;18, base;19, motor. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0031] With reference to Figure 1 , Figure 2 and Figure 4 , the utility model provides an embodiment: gene sequencing sample efficient pretreatment device, including installation box 1, the inner bottom wall left side of installation box 1 is fixedly connected with base 18, the top of base 18 is provided with processing tank 12, the bottom of processing tank 12 is installed with motor 19, through the installation of motor 19, give subsequent process with power, the output of motor 19 is fixedly connected with rotating rod 15, the equal interval fixed connection of rotating rod 15 outside has scraper 16, through the installation of scraper 16, avoid the inner wall of processing tank 12 to have residual, the top end of processing tank 12 is communicated with pipe 17, the top of installation box 1 is installed with cleaning mechanism 2, and cleaning mechanism 2 is used for the cleaning of installation box 1 inside, and the right side middle part of installation box 1 is fixedly connected with the clamping groove plate 6, and the inside of clamping groove plate 6 is installed with notice board 5, and the front side middle part of installation box 1 is installed with transparent plate 7;

[0032] Specifically, when carrying out sample pretreatment work, first, start motor 19, after the start of motor 19, drive rotating rod 15 to start rotating, and rotating rod 15 drives scraper 16 to rub the inner wall of processing tank 12 regularly, in this process, scraper 16 can ensure that the sample is uniformly mixed in processing tank 12, so that the various components in the sample are fully mixed, and meanwhile, effectively prevent the sample from being left in the processing process, and guarantee the quality of pretreatment, at the same time, pipe 17 undertakes the input and output work of sample, transports sample, so as to realize the efficient pretreatment of sample, and the installation of notice board 5 is convenient for reminding the operator of operation process and matters needing attention, and the installation of transparent plate 7 provides convenience for internal observation of equipment, and the operator can intuitively check the sample processing condition at any time.

[0033] With reference to Figure 1 , Figure 3 and Figure 5The cleaning mechanism 2 comprises a fixed plate 206 rotatably connected to the middle of the top end of the mounting box 1, a stirring box 201 mounted on the rear side of the fixed plate 206, a rotating shaft 204 rotatably connected to the middle of the stirring box 201, a plurality of stirring blades 205 fixedly connected to the outer side of the rotating shaft 204 at equal intervals, a plurality of nozzles 203 fixedly mounted on the front side of the fixed plate 206 at equal intervals, and a turntable 3 fixedly connected to the right side of the stirring box 201 through the rotating shaft 204, and a fixed short column 4 fixedly connected to the right side top of the turntable 3, so as to facilitate the rotation of the rotating shaft 204, and a handle 10 mounted on the rear side of the stirring box 201, screws 11 threadedly connected to the left and right ends of the handle 10, a circular cover 13 mounted on the rear side of the feeding pipe 202, and a mounting short column 14 fixedly connected to the rear side middle of the circular cover 13, so as to avoid dirt entering the stirring box 201;

[0034] Specifically, when the mounting box 1 is cleaned, first, the cleaning liquid is delivered into the stirring box 201 through the feeding pipe 202, then the turntable 3 is rotated to drive the rotating shaft 204 to start rotating, and the stirring blades 205 are driven to rotate at high speed, under the stirring of the stirring blades 205, the components in the cleaning liquid are fully mixed, so as to achieve the best cleaning effect, after the stirring process is completed, the fixed plate 206 is rotated to adjust the position of the stirring box 201, so that it is rotated to the appropriate angle, finally, the mixed cleaning material is sprayed out through the nozzles 203, so as to ensure that the mounting box 1 is thoroughly cleaned, and the inside of the mounting box 1 is fully cleaned.

[0035] Referring to Figure 1 , Figure 2 and Figure 3 , the left side middle of the mounting box 1 is fixedly connected with a mounting plate 8, and the left side of the mounting plate 8 is fixedly connected with a plurality of hooks 9.

[0036] Specifically, the hooks 9 are installed to facilitate the taking and use of parts.

[0037] Working principle: first, the motor 19 is started, the motor 19 drives the rotating rod 15 to rotate, the rotating action of the rotating rod 15 drives the scraper 16 to scrape on the inner wall of the treatment tank 12, so as to ensure that the sample can be uniformly mixed, and prevent the sample from being left during the treatment process, at the same time, the sample is input and output through the conduit 17, so as to realize efficient pretreatment of the sample, the installation of the signboard 5 facilitates the reminding of the workers, and the installation of the transparent plate 7 facilitates the observation of the inside.

[0038] The cleaning liquid is transported into the stirring box 201 through the feeding pipe 202, then the rotating shaft 204 is rotated to drive the stirring blade 205 to rotate, so that the stirring mixing is realized, thereby achieving the best cleaning effect; after the stirring is completed, the fixed plate 206 starts to rotate, so that the stirring box 201 can be rotated to a suitable position, thereby facilitating subsequent operation; finally, the mixed cleaning material is sprayed out through the nozzle 203, and the sprayed cleaning liquid uniformly covers the inside of the installation box 1, thereby realizing the overall cleaning of the inside of the installation box 1.

[0039] Finally, it should be noted that the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application are described in detail, for those skilled in the art, it still can be modified, or part of the technical features of the equivalent replacement, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, should be included within the scope of the present application.

Claims

1. A high-efficiency pretreatment device for gene sequencing samples, comprising a mounting box (1), characterized in that: The inner bottom wall left side of mounting box (1) is fixedly connected with base (18), the top of base (18) is provided with processing tank (12), the bottom of processing tank (12) is installed with motor (19), the output end of motor (19) is fixedly connected with rotating rod (15), the outer side of rotating rod (15) is fixedly connected with scraper (16) equidistantly, the top end of processing tank (12) is communicated with pipe (17), the top of mounting box (1) is installed with cleaning mechanism (2), and the cleaning mechanism (2) is used for cleaning inside the mounting box (1). 2.The gene sequencing sample high-efficiency pretreatment device according to claim 1, characterized in that: The cleaning mechanism (2) comprises a fixed plate (206), the fixed plate (206) is rotatably connected to the top end of the mounting box (1), the rear side of the fixed plate (206) is provided with a stirring box (201), the middle part of the stirring box (201) is rotatably connected with a rotating shaft (204), the outer side of the rotating shaft (204) is fixedly connected with a plurality of stirring blades (205), the rear side of the stirring box (201) is communicated with a feeding pipe (202), and the front side of the fixed plate (206) is fixedly connected with a plurality of nozzles (203). 3.The gene sequencing sample high-efficiency pretreatment device according to claim 2, characterized in that: The right side of the rotating shaft (204) penetrates the right side of the stirring box (201) and is fixedly connected with a rotating disc (3), and the right side top of the rotating disc (3) is fixedly connected with a fixed short column (4).

4. The high-efficiency pretreatment device for gene sequencing sample according to claim 2, characterized in that: The rear side of the stirring box (201) is provided with a handle (10), and the left and right ends of the handle (10) are screw-connected with screws (11).

5. The high-efficiency pretreatment device for gene sequencing sample according to claim 2, characterized in that: The rear side of the feeding pipe (202) is provided with a circular cover (13), and the rear side of the circular cover (13) is fixedly connected with a mounting short column (14).

6. The high-efficiency pretreatment device for gene sequencing sample according to claim 1, characterized in that: The right side of the mounting box (1) is fixedly connected with a clamping groove plate (6), and the inside of the clamping groove plate (6) is provided with a signboard (5).

7. The high-efficiency pretreatment device for gene sequencing sample according to claim 1, characterized in that: The front side of the mounting box (1) is provided with a transparent plate (7). 8.The gene sequencing sample high-efficiency pretreatment device according to claim 1, characterized in that: The left side of the mounting box (1) is fixedly connected with a mounting plate (8), and the left side of the mounting plate (8) is fixedly connected with a plurality of hooks (9) equidistantly. The left side of the mounting box (1) is fixedly connected with a mounting plate (8), and the left side of the mounting plate (8) is fixedly connected with a plurality of hooks (9) equidistantly.

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