96-well plate film sealing auxiliary device for PCR (Polymerase Chain Reaction) experiment
By designing a sealing device for 96-well plates used in PCR experiments, and utilizing a mounting base and sliding pressure section structure, efficient sealing of the 96-well plate by simultaneously scraping and pressing the four edges of the plate was achieved. This solves the problem of low sealing efficiency in existing technologies, ensures a firm bond between the sealing film and the edges of the plate, and prevents leakage of the reaction mixture.
Patent Information
- Application Number
- CN202520358807.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-01-23
- Estimated Expiration
- 2035-02-28
AI Technical Summary
In the existing technology, the efficiency of scraping and pressing the four edges of the 96-well plate sealing film is low, resulting in poor adhesion between the edge of the sealing film and the edge of the orifice plate, which easily leads to leakage of the reaction mixture.
A sealing aid device for 96-well plates used in PCR experiments was designed, including a mounting base, a first pressing part and a second pressing part. The first pressing part slides along the length of the well plate to scrape the two long edges, and the second pressing part slides along the width of the well plate to scrape the two wide edges. The roller structure is used to reduce friction and improve the scraping efficiency.
By simultaneously scraping and pressing the four edges of the 96-well plate, the efficiency of the sealing film is significantly improved, ensuring a firm bond between the sealing film and the edges of the well plate and preventing leakage of the reaction mixture.
Smart Images
Figure CN223822179U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biomedical technology, specifically to a sealing auxiliary device for 96-well plates used in PCR experiments. Background Technology
[0002] 96-well plates are a common tool in PCR experiments. During the PCR experiment, sealing film is needed to seal the reaction mixture on the 96-well plate. This sealing operation is necessary to ensure that the PCR reaction mixture is not contaminated or evaporated, thereby ensuring the successful execution of the PCR reaction.
[0003] like Figure 1 As shown, this is a commonly used 96-well plate, comprising a plate body 1 and 96 receiving tubes 2 formed on the plate body for holding PCR reaction mixtures. When sealing this 96-well plate, the experimenter first covers the upper side of the plate body 1 with the sealing film, and then uses a scraper to scrape and press the entire surface of the sealing film to adhere it to the plate body 1. During the PCR experiment, the edges of the sealing film are prone to lifting off the edge of the plate body, which can lead to leakage of the reaction mixture in the wells at the edges of the 96-well plate. Therefore, after scraping and pressing the entire surface of the sealing film with a scraper, it is necessary to focus on scraping and pressing the four edges of the sealing film (i.e., the two length edges and the two width edges) several times to ensure a firm adhesion between the edges of the sealing film and the edges of the 96-well plate.
[0004] Currently, when using a scraper to scrape and press the entire surface of the sealing film, the experimenters hold the scraper and scrape and press each of the four edges of the sealing film sequentially. This means that each scraping action can only scrape and press one edge of the sealing film, which is time-consuming and inefficient when scraping and pressing the four edges of the sealing film. Utility Model Content
[0005] In view of this, the purpose of this utility model is to provide an auxiliary device for sealing 96-well plates for PCR experiments, so as to solve the technical problem of low efficiency when scraping and pressing the four edges of the sealing film in the prior art.
[0006] This utility model is achieved through the following technical solution:
[0007] A sealing aid for a 96-well plate used in PCR experiments includes a mounting base, a first pressing part, and a second pressing part. The mounting base is used to install and position the 96-well plate. The first pressing part is slidably disposed on the mounting base along the length direction of the 96-well plate and has two first pressing heads, which are respectively used to press the sealing film at the two long edge positions of the 96-well plate. The second pressing part is slidably disposed on the mounting base along the width direction of the 96-well plate and has two second pressing heads, which are respectively used to press the sealing film at the two wide edge positions of the 96-well plate.
[0008] Furthermore, the 96-well plate includes a plate body and 96 receiving tubes formed on the plate body for containing PCR reaction mixture. The mounting base includes a mounting plate, the upper side of which is recessed downward to form a limiting groove that mates with the plate body. The bottom wall of the limiting groove has a receiving groove that mates with each receiving tube at the position of each receiving tube.
[0009] Furthermore, the mounting base also includes a first guide rail and a second guide rail, both of which are fixed on the mounting plate. The first guide rail is located on one side of the limiting groove and is arranged along the length direction of the limiting groove. The second guide rail is located on the other side of the limiting groove adjacent to the first side and is arranged along the width direction of the limiting groove.
[0010] The first pressing part includes a first movable plate, one end of which is connected to the first guide rail in a manner that allows it to slide along the length direction of the limiting groove; both first pressing heads are fixedly mounted on the first movable plate.
[0011] The second pressing part includes a second movable plate, one end of which is connected to the second guide rail in a manner that allows it to slide along the width direction of the limiting groove; both second pressing heads are fixedly mounted on the second movable plate.
[0012] Furthermore, a first groove is formed by recessing the side of the first guide rail facing the first moving plate. The first groove is arranged along the length direction of the limiting groove, and a first slider is slidably fitted inside the first groove. The first slider is fixedly connected to one end of the first moving plate.
[0013] The second guide rail has a second groove recessed inward on the side facing the second moving plate. The second groove is arranged along the width direction of the limiting groove. A second slider is slidably fitted in the second groove. The second slider is fixedly connected to one end of the second moving plate.
[0014] Furthermore, the opening size of the first chute is smaller than the cavity size; the opening size of the second chute is smaller than the cavity size.
[0015] Furthermore, both ends of the first chute are closed; both ends of the second chute are closed.
[0016] Furthermore, each of the two first pressure heads includes a first connecting part and a first roller. The two first connecting parts are fixedly connected to the first movable plate, and the two first rollers are rotatably connected to the two first connecting parts respectively. The circumferential surfaces of the two first rollers are respectively used to press the two long edge positions of the 96-hole plate.
[0017] Both second pressure heads include a second connecting part and a second roller. The two second connecting parts are fixedly connected to the second movable plate. The two second rollers are rotatably connected to the two second connecting parts respectively. The circumferential surfaces of the two second rollers are used to press the two wide edge positions of the 96-hole plate.
[0018] Furthermore, the first connecting part includes a first cylinder, a first plate, a second plate, a first bolt, a third plate, a first cylinder, a fourth plate, and a first helical spring. The first cylinder is fixed to the first movable plate. The first plate and the second plate are respectively fixed to the upper and lower ends of the first cylinder. A first screw hole is formed on the first plate, and a first through hole is formed on the second plate. The first bolt is screwed into the first screw hole. The third plate is slidably fitted inside the first cylinder. The upper side of the third plate abuts against the lower end of the first bolt. The first cylinder is slidably fitted inside the first through hole. The fourth plate is slidably fitted inside the first cylinder. The fourth plate is fixedly connected to the upper end of the first cylinder. The first helical spring is located inside the first cylinder. The upper end of the first helical spring abuts against the third plate, and the lower end abuts against the fourth plate. The first roller is rotatably connected to the first cylinder.
[0019] Furthermore, the second connecting part includes a second cylinder, a fifth plate, a sixth plate, a second bolt, a seventh plate, a second cylinder, an eighth plate, and a second helical spring. The second cylinder is fixed to the second movable plate. The fifth plate and the sixth plate are respectively fixed to the upper and lower ends of the second cylinder. A second screw hole is formed on the fifth plate, and a second through hole is formed on the sixth plate. The second bolt is screwed into the second screw hole. The seventh plate is slidably fitted inside the second cylinder. The upper side of the seventh plate abuts against the lower end of the second bolt. The second cylinder is slidably fitted inside the second through hole. The eighth plate is slidably fitted inside the second cylinder. The eighth plate is fixedly connected to the upper end of the second cylinder. The second helical spring is located inside the second cylinder. The upper end of the second helical spring abuts against the seventh plate, and the lower end abuts against the eighth plate. The second roller is rotatably connected to the second cylinder.
[0020] Furthermore, the first connecting part also includes a first horizontal plate, two first vertical plates and two first rotating shafts. The first horizontal plate is fixed to the lower end of the first cylinder, and the two first vertical plates are fixed to the lower side of the first horizontal plate. The two first vertical plates are parallel to each other, and each of the two first vertical plates has a first shaft hole. The two first shaft holes face each other, and the two first rotating shafts are rotatably fitted into the two first shaft holes respectively. The two first rotating shafts are fixedly connected to the middle of both ends of the first roller respectively, and the first rotating shafts are arranged along the width direction of the limiting groove.
[0021] The second connecting part further includes a second horizontal plate, two second vertical plates, and two second rotating shafts. The second horizontal plate is fixed to the lower end of the second cylinder, and the two second vertical plates are fixed to the lower side of the second horizontal plate. The two second vertical plates are parallel to each other, and each of the two second vertical plates has a second shaft hole. The two second shaft holes are directly opposite each other, and the two second rotating shafts are rotatably fitted into the two second shaft holes respectively. The two second rotating shafts are respectively fixedly connected to the middle of both ends of the second roller, and the second rotating shafts are arranged along the length direction of the limiting groove.
[0022] The beneficial effects of this utility model are as follows:
[0023] When using the PCR experimental 96-well plate sealing aid device of this invention to assist in sealing a 96-well plate, when the first pressing part slides along the length direction of the 96-well plate, one scraping action can simultaneously scrape the two long edges of the sealing film. When the second pressing part slides along the width direction of the 96-well plate, one scraping action can simultaneously scrape the two wide edges of the sealing film. With the assistance of the PCR experimental 96-well plate sealing aid device of this invention, it is more time-saving to scrape the four edges of the sealing film and can improve the scraping efficiency of the four edges of the sealing film.
[0024] Other advantages, objectives, and features of this invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination and study, or may be learned from practice of this invention. The objectives and other advantages of this invention can be realized and obtained through the following description. Attached Figure Description
[0025] Figure 1 This is a schematic diagram of a 96-well plate.
[0026] Figure 2 This is a schematic diagram of the sealing device for PCR experiments using the present invention.
[0027] Figure 3 This is a schematic diagram of the first pressure head in the sealing auxiliary device for PCR experiments of this utility model;
[0028] Figure 4 This is a front view of the first pressure head in the PCR experimental 96-well plate sealing auxiliary device of this utility model;
[0029] Figure 5 for Figure 4 AA section view;
[0030] Figure 6 This is a schematic diagram of the second pressure head in the sealing auxiliary device for PCR experiments of this utility model;
[0031] Figure 7 This is a right view of the second pressure head in the PCR experimental 96-well plate sealing auxiliary device of this utility model;
[0032] Figure 8 for Figure 7 BB section view;
[0033] Figure 9 This is a schematic diagram of the combined structure of the first guide rail and the first slider in the PCR experimental 96-well plate sealing auxiliary device of this utility model;
[0034] Figure 10 This is a top view of the combined structure of the first guide rail and the first slider in the sealing auxiliary device for PCR experiments of this utility model;
[0035] Figure 11 for Figure 10 CC section view;
[0036] Figure 12 This is a schematic diagram of the combined structure of the second guide rail and the second slider in the PCR experimental 96-well plate sealing auxiliary device of this utility model;
[0037] Figure 13 This is a top view of the combined structure of the second guide rail and the second slider in the sealing aid device for PCR experiments of this utility model;
[0038] Figure 14 for Figure 13 DD sectional view.
[0039] In the diagram: Plate-1; Receiving tube-2; Mounting base-3; First pressing part-4; Second pressing part-5; First pressing head-41; Second pressing head-51; Mounting plate-31; Limiting groove-32; Receiving groove-33; First guide rail-34; Second guide rail-35; First moving plate-42; Second moving plate-52; First sliding groove-341; First slider-342; Second sliding groove-351; Second slider-352; First connecting part-411; First roller-412; Second connecting part-511; Second roller-512; First cylinder-4111; First plate-4112; Second... Plate - 4113; First Bolt - 4114; Third Plate - 4115; First Cylinder - 4116; Fourth Plate - 4117; First Helical Spring - 4118; Second Cylinder - 5111; Fifth Plate - 5112; Sixth Plate - 5113; Second Bolt - 5114; Seventh Plate - 5115; Second Cylinder - 5116; Eighth Plate - 5117; Second Helical Spring - 5118; First Horizontal Plate - 4119; First Vertical Plate - 4120; First Rotating Shaft - 4121; Second Horizontal Plate - 5119; Second Vertical Plate - 5120; Second Rotating Shaft - 5121. Detailed Implementation
[0040] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.
[0041] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.
[0042] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.
[0043] In the above description of this utility model, it should be noted that the terms "one side," "the other side," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first," "second," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.
[0044] Furthermore, terms such as "identical" do not imply that components must be absolutely identical; minor differences are permissible. The term "perpendicular" simply means that the positional relationship between components is more perpendicular than "parallel," not that the structure must be perfectly perpendicular; a slight tilt is acceptable.
[0045] To clearly describe the sealing aid device for PCR experiments in this invention, the structure of the 96-well plate is briefly described first:
[0046] like Figure 1 As shown, it is a commonly used 96-well plate, including a plate body 1 and 96 containment tubes 2 formed on the plate body 1 for containing PCR reaction mixture.
[0047] Please see Figure 2-14 This utility model provides a technical solution: a sealing auxiliary device for a 96-well plate used in PCR experiments, including a mounting base 3, a first pressing part 4, and a second pressing part 5. The mounting base 3 is used to install and position the 96-well plate. The first pressing part 4 is slidably disposed on the mounting base 3 along the length direction of the 96-well plate. The first pressing part 4 has two first pressing heads 41, which are respectively used to press the sealing film at the two long edge positions of the 96-well plate. The second pressing part 5 is slidably disposed on the mounting base 3 along the width direction of the 96-well plate. The second pressing part 5 has two second pressing heads 51, which are respectively used to press the sealing film at the two wide edge positions of the 96-well plate.
[0048] When using the PCR experimental 96-well plate sealing aid device described in this invention to assist in sealing a 96-well plate, the 96-well plate is installed and positioned on the mounting base 3. Then, the release paper on the sealing film is peeled off, aligning the edge of the sealing film with the edge of the 96-well plate, and the sealing film is placed on the upper side of the 96-well plate. Then, a scraper is used to scrape and press the entire surface of the sealing film to adhere it to the plate body 1.
[0049] After scraping and pressing the entire surface of the sealing film with a scraper, the first pressing part 4 is repeatedly slid back and forth along the length direction of the 96-hole plate on the mounting base 3, so that the two first pressing heads 41 on the first pressing part 4 repeatedly scrape and press the sealing film at the two long edge positions of the 96-hole plate. Then, the second pressing part 5 is repeatedly slid back and forth along the width direction of the 96-hole plate on the mounting base 3, so that the two second pressing heads 51 on the second pressing part 5 repeatedly scrape and press the sealing film at the two wide edge positions of the 96-hole plate. This ensures that the edge of the sealing film is firmly bonded to the edge of the 96-hole plate. The sealing operation of the 96-hole plate is then completed.
[0050] After scraping the entire surface of the sealing film using a scraper, the first pressing part can simultaneously scrape the sealing film at the two long edges of the 96-well plate, and the second pressing part can simultaneously scrape the sealing film at the two wide edges of the 96-well plate. Specifically, when the first pressing part 4 slides along the length of the 96-well plate, one scraping action can simultaneously scrape the two long edges of the sealing film; when the second pressing part 5 slides along the width of the 96-well plate, one scraping action can simultaneously scrape the two wide edges of the sealing film. With the assistance of the PCR experimental 96-well plate sealing film auxiliary device described in this invention, scraping the four edges of the sealing film is more time-saving and can improve the scraping efficiency of the four edges of the sealing film.
[0051] In this embodiment, the mounting base 3 includes a mounting plate 31. The upper side of the mounting plate 31 is recessed downward to form a limiting groove 32 that cooperates with the plate body 1. The bottom wall of the limiting groove 32 is formed with a receiving groove 33 that cooperates with each receiving tube 2 at the position of each receiving tube 2.
[0052] The 96-hole plate is placed on the mounting plate 31, with each receiving tube 2 positioned within its corresponding receiving groove 33, and the plate body 1 positioned within its limiting groove 32. This completes the installation and positioning of the 96-hole plate on the mounting base 3. After the 96-hole plate is installed and positioned on the mounting base 3, it cannot move horizontally. With this structure, the mounting base 3 can effectively position the 96-hole plate.
[0053] In this embodiment, the mounting base 3 further includes a first guide rail 342 and a second guide rail 352. The first guide rail 342 and the second guide rail 352 are both fixed on the mounting plate 31. The first guide rail 342 is located on one side of the limiting groove 32 and is arranged along the length direction of the limiting groove 32. The second guide rail 352 is located on the other side of the limiting groove 32 adjacent to the first side and is arranged along the width direction of the limiting groove 32.
[0054] The first pressing part 4 includes a first movable plate 42, one end of which is connected to the first guide rail 342 in a manner that allows it to slide along the length direction of the limiting groove 32; both first pressing heads 41 are fixed on the first movable plate 42.
[0055] The second pressing part 5 includes a second movable plate 52, one end of which is connected to the second guide rail 352 in a manner that allows it to slide along the width direction of the limiting groove 32; both second pressing heads 51 are fixed on the second movable plate 52.
[0056] Since the limiting groove 32 cooperates with the plate 1, the length direction of the limiting groove 32 is the length direction of the 96-hole plate, and the width direction of the limiting groove 32 is the width direction of the 96-hole plate.
[0057] Since one end of the first movable plate 42 is connected to the first guide rail 342 in a manner that allows it to slide along the length direction of the limiting groove 32, the first pressing part 4 can be slidably mounted on the mounting base 3 in a manner that allows it to slide along the length direction of the 96-hole plate. Since one end of the second movable plate 52 is connected to the second guide rail 352 in a manner that allows it to slide along the width direction of the limiting groove 32, the second pressing part 5 can be slidably mounted on the mounting base 3 in a manner that allows it to slide along the width direction of the 96-hole plate.
[0058] In this embodiment, the side of the first guide rail 342 facing the first moving plate 42 is recessed inward to form a first groove 341. The first groove 341 is arranged along the length direction of the limiting groove 32, and a first slider 342 is slidably fitted within the first groove 341. One end of the first moving plate 42 is fixedly connected to the first slider 342. With this structure, one end of the first moving plate 42 can be connected to the first guide rail 342 in a manner that allows it to slide along the length direction of the limiting groove 32.
[0059] The second guide rail 352 has a recessed second groove 351 on its side facing the second moving plate 52. The second groove 351 is arranged along the width direction of the limiting groove 32, and a second slider 352 is slidably fitted within the second groove 351. One end of the second moving plate 52 is fixedly connected to the second slider 352. With this structure, one end of the second moving plate 52 can be connected to the second guide rail 352 in a manner that allows it to slide along the width direction of the limiting groove 32.
[0060] In this embodiment, the opening size of the first slide groove 341 is smaller than the cavity size; the opening size of the second slide groove 351 is smaller than the cavity size. Specifically, the cross-sections of both the first slide groove 341 and the second slide groove 351 are dovetail-shaped. With this structure, the opening size of the first slide groove 341 and the opening size of the second slide groove 351 can be smaller than the cavity size.
[0061] Because the opening size of the first groove 341 is smaller than the cavity size, the first slider 342 slides within the first groove 341, preventing it from dislodging from the opening. Similarly, because the opening size of the second groove 351 is smaller than the cavity size, the second slider 352 slides within the second groove 351, preventing it from dislodging. This structure provides good structural stability for the 96-well plate sealing aid for PCR experiments described in this invention.
[0062] In this embodiment, both ends of the first slide groove 341 are closed; both ends of the second slide groove 351 are also closed. This structure prevents the first slider 342 from disengaging from both ends of the first slide groove 341, and prevents the second slider 352 from disengaging from both ends of the second slide groove 351. This further improves the structural stability of the PCR experimental 96-well plate sealing auxiliary device described in this invention.
[0063] In this embodiment, each of the two first pressure heads 41 includes a first connecting portion 411 and a first roller 412. The two first connecting portions 411 are fixedly connected to the first movable plate 42, and the two first rollers 412 are rotatably connected to the two first connecting portions 411 respectively. The circumferential surfaces of the two first rollers 412 are respectively used to press the two long edge positions of the 96-hole plate. Specifically, the rotation center line of the first rollers 412 is set along the width direction of the 96-hole plate. When the two first rollers 412 move along the length direction of the 96-hole plate to the 96-hole plate, the two first rollers 412 press the two long edge positions of the 96-hole plate respectively.
[0064] When the first pressing part 4 needs to slide along the length of the 96-hole plate on the mounting base 3, the first moving plate 42 slides along the length of the 96-hole plate. The first moving plate 42 drives the first connecting part 411 to slide along the length of the 96-hole plate. The first connecting part 411 drives the two first rollers 412 to slide along the length of the 96-hole plate. During the process of the two first rollers 412 sliding along the length of the 96-hole plate, the two first rollers 412 can roll on the two long edges of the 96-hole plate respectively to crush the two long edges of the 96-hole plate. With this structure, the first pressing head 41 can scrape and press the long edges of the 96-hole plate. During the process of the first pressure head 41 scraping the edge of the 96-hole plate, the first roller 412 can roll on the sealing film on the edge of the 96-hole plate, so that the friction between the first pressure head 41 and the sealing film is rolling friction, which reduces the friction between the first pressure head 41 and the sealing film and reduces the possibility of damage to the sealing film.
[0065] Both second pressure heads 51 include a second connecting portion 511 and a second roller 512. The two second connecting portions 511 are fixedly connected to the second movable plate 52, and the two second rollers 512 are rotatably connected to the two second connecting portions 511 respectively. The circumferential surfaces of the two second rollers 512 are respectively used to press the two wide edges of the 96-hole plate. Specifically, the rotation center line of the second rollers 512 is set along the length direction of the 96-hole plate. When the two second rollers 512 move along the width direction of the 96-hole plate onto the 96-hole plate, the two second rollers 512 respectively press the two wide edges of the 96-hole plate.
[0066] When the second pressing part 5 needs to slide along the width direction of the 96-hole plate on the mounting base 3, the second moving plate 52 slides along the width direction of the 96-hole plate. The second moving plate 52 drives the second connecting part 511 to slide along the width direction of the 96-hole plate. The second connecting part 511 drives the two second rollers 512 to slide along the width direction of the 96-hole plate. During the process of the two second rollers 512 sliding along the width direction of the 96-hole plate, the two second rollers 512 can roll on the two wide edges of the 96-hole plate respectively to crush the two wide edges of the 96-hole plate. With this structure, the second pressing head 51 can scrape and press the wide edges of the 96-hole plate. During the process of the second pressure head 51 scraping the edge of the 96-hole plate, the second roller 512 can roll on the sealing film on the edge of the 96-hole plate, so that the friction between the second pressure head 51 and the sealing film is rolling friction, which reduces the friction between the second pressure head 51 and the sealing film and reduces the possibility of damage to the sealing film.
[0067] In this embodiment, the first connecting part 411 includes a first cylinder 4111, a first plate 4112, a second plate 4113, a first bolt 4114, a third plate 4115, a first cylinder 4116, a fourth plate 4117, and a first helical spring 4118. The first cylinder 4111 is fixed to the first movable plate 42. The first plate 4112 and the second plate 4113 are respectively fixed to the upper and lower ends of the first cylinder 4111. A first screw hole is formed on the first plate 4112, and a first through hole is formed on the second plate 4113. The first bolt 4114 is screwed into the first screw hole. The third plate 4115... The third plate 4115 is slidably fitted inside the first cylinder 4111. The upper side of the third plate 4115 abuts against the lower end of the first bolt 4114. The first cylinder 4116 is slidably fitted inside the first through hole. The fourth plate 4117 is slidably fitted inside the first cylinder 4111. The fourth plate 4117 is fixedly connected to the upper end of the first cylinder 4116. The first helical spring 4118 is located inside the first cylinder 4111. The upper end of the first helical spring 4118 abuts against the third plate 4115, and the lower end abuts against the fourth plate 4117. The first roller 412 is rotatably connected to the first cylinder 4116.
[0068] Before sliding the first pressing part 4 along the length of the 96-hole plate on the mounting base 3, the first bolt 4114 can be rotated in the first screw hole to move the screw-in end of the first bolt 4114 upward or downward, thereby adjusting the distance between the screw-in end of the first bolt 4114 and the fourth plate 4117, adjusting the distance between the third plate 4115 and the fourth plate 4117, adjusting the degree of compression of the first helical spring 4118, adjusting the downward pressure generated by the first helical spring 4118 on the fourth plate 4117, and further adjusting the pressure of the first roller 412 on the sealing film.
[0069] In this embodiment, the second connecting part 511 includes a second cylinder 5111, a fifth plate 5112, a sixth plate 5113, a second bolt 5114, a seventh plate 5115, a second cylinder 5116, an eighth plate 5117, and a second helical spring 5118. The second cylinder 5111 is fixed to the second movable plate 52. The fifth plate 5112 and the sixth plate 5113 are respectively fixed to the upper and lower ends of the second cylinder 5111. A second screw hole is formed on the fifth plate 5112, and a second through hole is formed on the sixth plate 5113. The second bolt 5114 is screwed into the second screw hole. The seventh plate 5115... The seventh plate 5115 is slidably fitted inside the second cylinder 5111. The upper side of the seventh plate 5115 abuts against the lower end of the second bolt 5114. The second cylinder 5116 is slidably fitted inside the second through hole. The eighth plate 5117 is slidably fitted inside the second cylinder 5111. The eighth plate 5117 is fixedly connected to the upper end of the second cylinder 5116. The second helical spring 5118 is located inside the second cylinder 5111. The upper end of the second helical spring 5118 abuts against the seventh plate 5115, and the lower end abuts against the eighth plate 5117. The second roller 512 is rotatably connected to the second cylinder 5116.
[0070] Before sliding the second pressing part 5 along the width direction of the 96-hole plate on the mounting base 3, the second bolt 5114 can be rotated in the second screw hole to move the screw-in end of the second bolt 5114 upward or downward, thereby adjusting the distance between the screw-in end of the second bolt 5114 and the eighth plate 5117, adjusting the distance between the seventh plate 5115 and the eighth plate 5117, adjusting the degree of compression of the second helical spring 5118, adjusting the downward pressure generated by the second helical spring 5118 on the eighth plate 5117, and further adjusting the pressure of the second roller 512 on the sealing film.
[0071] In this embodiment, the first connecting portion 411 further includes a first horizontal plate 4119, two first vertical plates 4120, and two first rotating shafts 4121. The first horizontal plate 4119 is fixed to the lower end of the first cylinder 4116, and the two first vertical plates 4120 are both fixed to the lower side of the first horizontal plate 4119. The two first vertical plates 4120 are parallel to each other, and each of the two first vertical plates 4120 has a first shaft hole, which is directly opposite to each other. The two first rotating shafts 4121 are rotatably fitted into the two first shaft holes, and the two first rotating shafts 4121 are respectively fixedly connected to the middle of both ends of the first roller 412. The first rotating shafts 4121 are arranged along the width direction of the limiting groove 32. With this structure, the first roller 412 can be rotatably connected to the first connecting portion 411, and the rotation center line of the first roller 412 can be arranged along the width direction of the 96-hole plate.
[0072] The second connecting part 511 further includes a second horizontal plate 5119, two second vertical plates 5120, and two second rotating shafts 5121. The second horizontal plate 5119 is fixed to the lower end of the second cylinder 5116, and the two second vertical plates 5120 are both fixed to the lower side of the second horizontal plate 5119. The two second vertical plates 5120 are parallel to each other, and each of the two second vertical plates 5120 has a second shaft hole formed on it. The two second shaft holes face each other, and the two second rotating shafts 5121 are rotatably fitted into the two second shaft holes respectively. The two second rotating shafts 5121 are respectively fixedly connected to the middle of both ends of the second roller 512, and the second rotating shafts 5121 are arranged along the length direction of the limiting groove 32. With this structure, the second roller 512 can be rotatably connected to the second connecting part 511, and the rotation center line of the second roller 512 can be arranged along the length direction of the 96-hole plate.
[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this utility model without departing from the spirit and scope of the technical solutions of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.
Claims
1. A sealing aid for a 96-well plate used in PCR experiments, characterized in that: The device includes a mounting base, a first pressing part, and a second pressing part. The mounting base is used for mounting and positioning a 96-hole plate. The first pressing part is slidably disposed on the mounting base along the length direction of the 96-hole plate and has two first pressing heads, which are respectively used to press the sealing film at the two long edge positions of the 96-hole plate. The second pressing part is slidably disposed on the mounting base along the width direction of the 96-hole plate and has two second pressing heads, which are respectively used to press the sealing film at the two wide edge positions of the 96-hole plate.
2. The sealing aid device for a 96-well plate for PCR experiments according to claim 1, wherein the 96-well plate comprises a plate body and 96 receiving tubes formed on the plate body for containing the PCR reaction mixture, characterized in that: The mounting base includes a mounting plate, the upper side of which is recessed downward to form a limiting groove that mates with the plate body, and the bottom wall of the limiting groove has a receiving groove that mates with each receiving tube at the position of each receiving tube.
3. The PCR experimental 96-well plate sealing aid device according to claim 2, characterized in that: The mounting base also includes a first guide rail and a second guide rail. The first guide rail and the second guide rail are both fixed on the mounting plate. The first guide rail is located on one side of the limiting groove and is arranged along the length direction of the limiting groove. The second guide rail is located on the other side of the limiting groove adjacent to the first side and is arranged along the width direction of the limiting groove. The first pressing part includes a first movable plate, one end of which is connected to the first guide rail in a manner that allows it to slide along the length direction of the limiting groove; both first pressing heads are fixedly mounted on the first movable plate. The second pressing part includes a second movable plate, one end of which is connected to the second guide rail in a manner that allows it to slide along the width direction of the limiting groove; both second pressing heads are fixedly mounted on the second movable plate.
4. The PCR experimental 96-well plate sealing aid device according to claim 3, characterized in that: The first guide rail has a first groove recessed inward on the side facing the first moving plate. The first groove is arranged along the length direction of the limiting groove. A first slider is slidably fitted in the first groove. The first slider is fixedly connected to one end of the first moving plate. The second guide rail has a second groove recessed inward on the side facing the second moving plate. The second groove is arranged along the width direction of the limiting groove. A second slider is slidably fitted in the second groove. The second slider is fixedly connected to one end of the second moving plate.
5. The PCR experimental 96-well plate sealing aid device according to claim 4, characterized in that: The opening size of the first chute is smaller than the cavity size; the opening size of the second chute is smaller than the cavity size.
6. The PCR experimental 96-well plate sealing aid device according to claim 4, characterized in that: Both ends of the first slide are closed; both ends of the second slide are closed.
7. The PCR experimental 96-well plate sealing aid device according to claim 3, characterized in that: Both first pressure heads include a first connecting part and a first roller. The two first connecting parts are fixedly connected to the first movable plate. The two first rollers are rotatably connected to the two first connecting parts respectively. The circumferential surfaces of the two first rollers are respectively used to press the two long edge positions of the 96-hole plate. Both second pressure heads include a second connecting part and a second roller. The two second connecting parts are fixedly connected to the second movable plate. The two second rollers are rotatably connected to the two second connecting parts respectively. The circumferential surfaces of the two second rollers are used to press the two wide edge positions of the 96-hole plate.
8. The PCR experimental 96-well plate sealing aid device according to claim 7, characterized in that: The first connecting part includes a first cylinder, a first plate, a second plate, a first bolt, a third plate, a first cylinder, a fourth plate, and a first helical spring. The first cylinder is fixed to the first movable plate. The first plate and the second plate are respectively fixed to the upper and lower ends of the first cylinder. A first screw hole is formed on the first plate, and a first through hole is formed on the second plate. The first bolt is screwed into the first screw hole. The third plate is slidably fitted inside the first cylinder. The upper side of the third plate abuts against the lower end of the first bolt. The first cylinder is slidably fitted inside the first through hole. The fourth plate is slidably fitted inside the first cylinder. The fourth plate is fixedly connected to the upper end of the first cylinder. The first helical spring is located inside the first cylinder. The upper end of the first helical spring abuts against the third plate, and the lower end abuts against the fourth plate. The first roller is rotatably connected to the first cylinder.
9. The PCR experimental 96-well plate sealing aid device according to claim 8, characterized in that: The second connecting part includes a second cylinder, a fifth plate, a sixth plate, a second bolt, a seventh plate, a second cylinder, an eighth plate, and a second helical spring. The second cylinder is fixed to the second movable plate. The fifth plate and the sixth plate are respectively fixed to the upper and lower ends of the second cylinder. A second screw hole is formed on the fifth plate, and a second through hole is formed on the sixth plate. The second bolt is screwed into the second screw hole. The seventh plate is slidably fitted inside the second cylinder. The upper side of the seventh plate abuts against the lower end of the second bolt. The second cylinder is slidably fitted inside the second through hole. The eighth plate is slidably fitted inside the second cylinder. The eighth plate is fixedly connected to the upper end of the second cylinder. The second helical spring is located inside the second cylinder. The upper end of the second helical spring abuts against the seventh plate, and the lower end abuts against the eighth plate. The second roller is rotatably connected to the second cylinder.
10. The sealing device for 96-well plates used in PCR experiments according to claim 9, characterized in that: The first connecting part further includes a first horizontal plate, two first vertical plates and two first rotating shafts. The first horizontal plate is fixed to the lower end of the first cylinder. The two first vertical plates are fixed to the lower side of the first horizontal plate. The two first vertical plates are parallel to each other. A first shaft hole is formed on each of the two first vertical plates. The two first shaft holes are directly opposite each other. The two first rotating shafts are rotatably fitted into the two first shaft holes respectively. The two first rotating shafts are fixedly connected to the middle of both ends of the first roller respectively. The first rotating shafts are arranged along the width direction of the limiting groove. The second connecting part further includes a second horizontal plate, two second vertical plates, and two second rotating shafts. The second horizontal plate is fixed to the lower end of the second cylinder, and the two second vertical plates are fixed to the lower side of the second horizontal plate. The two second vertical plates are parallel to each other, and each of the two second vertical plates has a second shaft hole. The two second shaft holes are directly opposite each other, and the two second rotating shafts are rotatably fitted into the two second shaft holes respectively. The two second rotating shafts are respectively fixedly connected to the middle of both ends of the second roller, and the second rotating shafts are arranged along the length direction of the limiting groove.