Fluid control device for emulsifying fat and extracting adipose-derived stem cells

Abstract

The utility model discloses a flow control device for emulsifying fat and extracting adipose-derived stem cells, and belongs to the technical field of cells. The device comprises an emulsifying device and a filtering device, the emulsifying device comprises a first expansion section, a contraction section and a second expansion section which are connected in sequence; the filtering device comprises a cover plate, a first gasket, a second gasket, a third gasket, a slide and a bottom plate which are connected in sequence; according to the fluid device system disclosed by the utility model, efficient machining of adipose tissues is realized through optimized emulsification, micronization and filtration design. Compared with a traditional manual method, the recovery rate of stem cells and progenitor cells is improved. Due to the automatic design, human factors in operation are reduced, and the reliability of the system and the consistency of processing results are remarkably improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell technical field, concretely relates to a fat emulsification and extraction of fat stem cell's flow control device. BACKGROUND

[0002] Adipose-derived stem cells (ADSCs) have been widely used in the field of regenerative medicine due to their strong regenerative potential. Traditional methods of processing adipose tissue, such as enzymatic digestion and mechanical processing, can isolate stem cells from adipose tissue, but these methods have problems such as complex operation, unstable effect, and variability caused by manual operation. Especially, the existing mechanical method often needs multiple manual operations and complex laboratory equipment, which not only reduces the efficiency, but also increases the difficulty and risk of clinical application. SUMMARY

[0003] The utility model aims at providing a kind of fat emulsification and extraction of fat stem cell's flow control device, to realize the efficient processing of adipose tissue, production can be directly used for treatment based on cell injection preparation. By introducing adjustable fluid mechanics structure, dynamic filtration system and intelligent control technology, the utility model aims at improving the recovery rate of stem cells and progenitor cells, reducing the uncertainty caused by manual operation, improving the efficiency, stability and safety of overall processing process.

[0004] To solve the above technical problems, the utility model provides a kind of fat emulsification and extraction of fat stem cell's flow control device, including emulsification device and filter device;

[0005] The emulsification device includes first expansion section, contraction section and second expansion section connected in sequence;The first expansion section is provided with emulsification inlet;The second expansion section is provided with emulsification outlet;

[0006] The filter device includes cover plate, first gasket, second gasket, third gasket, glass slide and bottom plate connected in sequence;

[0007] The cover plate is provided with first inlet, second inlet and cell outlet;The emulsification outlet is communicated with first inlet and second inlet;

[0008] The first gasket is provided with first flow chamber;The first inlet and second inlet are communicated with both ends of first flow chamber respectively;

[0009] The second gasket is provided with second flow chamber;The second flow chamber is communicated with first flow chamber, and filter membrane is installed in second flow chamber, and filter hole is formed in filter membrane;

[0010] The third gasket is provided with partition hole;The partition hole is communicated with filter membrane;

[0011] The slide is provided with a cell culture area and a liquid outlet in communication; the cell culture area is in communication with the separation hole, and the liquid outlet is in communication with the cell outlet.

[0012] Preferably, a micro-vibrator or an ultrasonic generator is installed in the first and second expansion sections.

[0013] Preferably, the first flow chamber has a structure of narrow ends and a wide middle.

[0014] Preferably, the projection of the second flow chamber is located in the projection of the first flow chamber.

[0015] The second flow chamber is in communication with the middle part of the first flow chamber.

[0016] Preferably, the number of the filter membranes is two.

[0017] The pore size of the filter holes of the upper filter membrane is larger than that of the lower filter membrane.

[0018] Preferably, the pore sizes of the filter holes of the two filter membranes are 1 mm and 0.5 mm respectively.

[0019] Preferably, the sizes of the second flow chamber, the filter membrane, the separation hole and the flow channel are the same and corresponding.

[0020] Preferably, the first, second and third gaskets are all provided with outlet channels.

[0021] The liquid outlet is in communication with the outlet through the outlet channel.

[0022] Compared with the prior art, the fluid device system has the beneficial effects that:

[0023] The fluid device system of the utility model realizes the efficient mechanical processing of adipose tissue through the optimized emulsification, micronization and filtration design. Compared with the traditional manual method, the recovery rate of stem cells and progenitor cells is improved. The automation design reduces the human factors in operation, significantly improves the reliability of the system and the consistency of the processing results. In addition, the design of the whole system meets the clinical application requirements, is convenient to operate and popularize, and provides an efficient and reliable technical platform for autologous cell therapy in regenerative medicine. BRIEF DESCRIPTION OF DRAWINGS

[0024] The specific embodiments of the utility model will be further described in detail below with reference to the drawings.

[0025] Figure 1 is a structural schematic view of a fluid device for fat emulsification and extraction of adipose-derived stem cells.

[0026] In the drawings:

[0027] 1-Emulsification device; 11-First expansion section; 12-Contraction section; 13-Second expansion section; 111-Emulsification inlet; 131-Emulsification outlet; 2-Cover plate; 21-First inlet; 22-Second inlet; 23-Cell outlet; 3-First gasket; 31-First flow chamber; 4-Second gasket; 41-Second flow chamber; 5-Third gasket; 51-Separation hole; 6-Slide; 61-Cell culture area; 62-Outlet; 7-Base plate; 8-Filter membrane. Detailed Implementation

[0028] Many specific details are set forth in the following description to provide a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0029] The terminology used in one or more embodiments of this specification is for the purpose of describing particular embodiments only and is not intended to be limiting of the one or more embodiments of this specification. The singular forms “a,” “described,” and “the” as used in one or more embodiments of this specification and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. It should also be understood that the term “and / or” as used in one or more embodiments of this specification refers to and includes any or all possible combinations of one or more associated listed items.

[0030] It should be understood that although the terms first, second, etc., may be used to describe various information in one or more embodiments of this specification, such information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another. For example, first may also be referred to as second without departing from the scope of one or more embodiments of this specification, and similarly, second may also be referred to as first. Depending on the context, the word "if" as used herein may be interpreted as "when," "when," or "in response to a determination."

[0031] The present invention will now be described in further detail with reference to the accompanying drawings:

[0032] This utility model provides a flow control device for fat emulsification and extraction of adipose stem cells, including an emulsification device 1 and a filtration device;

[0033] The emulsifying device 1 includes a first expansion section 11, a contraction section 12, and a second expansion section 13 connected in sequence; the first expansion section 11 is provided with an emulsification inlet 111, and the second expansion section 13 is provided with an emulsification outlet 131;

[0034] The filter device includes a cover plate 2, a first gasket 3, a second gasket 4, a third gasket 5, a glass slide 6, and a base plate 7 connected in sequence.

[0035] The cover plate 2 is provided with a first inlet 21, a second inlet 22 and a cell outlet 23; the emulsification outlet 131 is connected to the first inlet 21 and the second inlet 22.

[0036] The first gasket 3 has a first flow chamber 31; the first inlet 21 and the second inlet 22 are respectively connected to the two ends of the first flow chamber 31;

[0037] The second gasket 4 has a second flow chamber 41; the second flow chamber 41 is connected to the first flow chamber 31, and a filter membrane 8 is installed in the second flow chamber 41, and the filter membrane 8 has filter holes.

[0038] The third gasket 5 has a partition hole 51; the partition hole 51 is connected to the filter membrane 8.

[0039] The glass slide 6 has a cell culture area 61 and a liquid outlet 62 that are connected to each other; the cell culture area 61 is connected to the separator 51, and the liquid outlet 62 is connected to the cell outlet 23.

[0040] Preferably, a micro-vibrator or an ultrasonic generator is installed in the first expansion section 11 and the second expansion section 13.

[0041] Preferably, the first flow chamber 31 has a structure that is narrow at both ends and wide in the middle.

[0042] Preferably, the projection of the second flow chamber 41 is located within the projection of the first flow chamber 31;

[0043] The second flow chamber 41 is connected to the middle part of the first flow chamber 31.

[0044] Preferably, the number of filter membranes 8 is two layers;

[0045] The pore size of the upper filter membrane 8 is larger than that of the lower filter membrane 8.

[0046] Preferably, the pore sizes of the two filter membranes 8 are 1 mm and 0.5 mm, respectively.

[0047] Preferably, the second flow chamber 41, the filter membrane 8, the separator 51, and the flow channel are of the same size and correspond to each other.

[0048] Preferably, the first gasket 3, the second gasket 4, and the third gasket 5 are all provided with outlet channels;

[0049] The liquid outlet 62 is connected to the outlet through the outlet channel.

[0050] To better illustrate the technical effects of this utility model, the present utility model provides the following specific embodiments to explain the above technical process:

[0051] Example 1: The fluid device system of this utility model consists of two main modules: an emulsification device and a filtration device, and its structure is as follows:

[0052] Emulsification device 1 comprises a first expansion section 11, a contraction section 12, and a second expansion section 13 connected in sequence; it employs adjustable diameter contraction regions (contraction section 12) and expansion regions (first expansion section 11 and second expansion section 13). Through a mechanical or hydraulic control system, the diameter of the flow channels can be adjusted in real time according to the characteristics of adipose tissue, thereby achieving more precise shear force and turbulent mixing, ensuring uniform micronization of the adipose tissue. The multi-segment contraction-expansion structure allows independent adjustment of the shear force and mixing effect of each segment, ensuring optimal tissue uniformity and emulsification effect at each stage throughout the entire processing. The device integrates a micro-vibrator or ultrasonic generator to prevent blockage within the device by generating micro-vibrations, ensuring continuous operation under high flow rates and high pressures.

[0053] The filtration device comprises a cover plate 2, a first gasket 3, a second gasket 4, a third gasket 5, a glass slide 6, and a base plate 7 connected in sequence. Adipose tissue flowing from the emulsification device 1 flows through the first inlet 21 and / or the second inlet 22 into both ends of the first flow chamber 31, then flows towards the middle of the first flow chamber 31, and flows into the filter membrane 8 of the second flow chamber 41 for filtration. After filtration, it passes through the separator 51 into the cell culture area 61, and finally flows out from the cell outlet 23 through the outlet 62. The filtration device employs a dynamic filtration system, using electromagnetic or piezoelectric materials to generate micro-vibrations on the surface of the filter membrane 8, preventing adipose tissue from accumulating and clogging the membrane surface, thereby maintaining efficient filtration. The device contains multiple layers of filter membranes 8, with pore sizes gradually decreasing from 1 mm to 0.5 mm, filtering adipose tissue step by step to ensure maximum retention of stem cells. The filter cartridge module adopts a replaceable design, allowing for quick replacement when the filter cartridge becomes clogged or reaches its service life, ensuring continuous and efficient operation of the filtration system.

[0054] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any changes or substitutions within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A flow control device for fat emulsification and extraction of adipose-derived stem cells, characterized in that: Includes an emulsifying device (1) and a filtering device; The emulsifying device (1) includes a first expansion section (11), a contraction section (12), and a second expansion section (13) connected in sequence; the first expansion section (11) is provided with an emulsification inlet (111), and the second expansion section (13) is provided with an emulsification outlet (131); The filter device includes a cover plate (2), a first gasket (3), a second gasket (4), a third gasket (5), a glass slide (6), and a base plate (7) connected in sequence; The cover plate (2) is provided with a first inlet (21), a second inlet (22) and a cell outlet (23); the emulsification outlet (131) is connected to the first inlet (21) and the second inlet (22); The first gasket (3) has a first flow chamber (31); the first inlet (21) and the second inlet (22) are respectively connected to the two ends of the first flow chamber (31); The second gasket (4) has a second flow chamber (41) connected to the first flow chamber (31). A filter membrane (8) is installed in the second flow chamber (41), and the filter membrane (8) has filter holes. The third gasket (5) has a partition hole (51); the partition hole (51) is connected to the filter membrane (8); The glass slide (6) has a cell culture area (61) and a liquid outlet (62) that are connected to each other; the cell culture area (61) is connected to the separator (51), and the liquid outlet (62) is connected to the cell outlet (23).

2. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 1, characterized in that: Micro-vibrators or ultrasonic generators are installed in the first expansion section (11) and the second expansion section (13).

3. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 2, characterized in that: The first flow chamber (31) has a structure that is narrow at both ends and wide in the middle.

4. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 3, characterized in that: The projection of the second flow chamber (41) is located within the projection of the first flow chamber (31); The second flow chamber (41) is connected to the middle part of the first flow chamber (31).

5. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 4, characterized in that: The number of filter membranes (8) is two; The pore size of the upper filter membrane (8) is larger than that of the lower filter membrane (8).

6. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 5, characterized in that: The pore sizes of the two filter membranes (8) are 1 mm and 0.5 mm, respectively.

7. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 6, characterized in that: The second flow chamber (41), filter membrane (8), separator hole (51) and flow channel are the same size and correspond to each other.

8. The flow control device for fat emulsification and extraction of adipose-derived stem cells according to claim 7, characterized in that: The first gasket (3), the second gasket (4), and the third gasket (5) are all provided with outlet channels; The liquid outlet (62) is connected to the outlet through the outlet channel.

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