Rapid diagnosis device for blood infection pathogens

Abstract

The utility model relates to the technical field of blood infection pathogen detection, and discloses a rapid diagnosis device for blood infection pathogens. The problems that in the prior art, special large instruments are needed, operation is tedious and complex, the technical difficulty is large, and clinical popularization and application are difficult are solved. A gene chip technology is high in hybridization background and difficult to eliminate, and the technical problems that the false positive rate is high, the technical difficulty is large and the cost is high exist. The device comprises a piezoelectric gene sensor, the piezoelectric gene sensor comprises a detection microarray, and the detection microarray is connected with an oscillating circuit, a frequency counting module, a shockproof module and a temperature control module; the detection microarray comprises a detection substrate, an electrode is arranged on the detection substrate, and a plurality of piezoelectric sensing units are installed on the detection substrate in an array mode. According to the utility model, pathogens in blood can be rapidly and sensitively detected, an accurate result can be given in a short time, and rapid response under emergency circumstances is facilitated.

Description

TECHNICAL FIELD

[0001] The utility model relates to blood infection pathogen detection technical field especially relates to a blood infection pathogen rapid diagnosis device. BACKGROUND

[0002] In recent years, with the increase of antitumor drugs, immunosuppressive agents and other drugs, and the increase of invasive operations such as artificial valve, artificial joint and indwelling venous catheter, the application range of various interventional diagnosis and treatment methods is increasingly expanded, and the population is aging and the emergence of immunocompromised population, the incidence rate of blood infection in hospitals is increasing year by year, which seriously threatens human health. Because the blood infection disease is insidious, there is no specific indication in clinic, so the detection of pathogenic bacteria is the only method for diagnosing blood infection. At present, the detection methods commonly used for blood infection pathogenic bacteria in clinic mainly include two categories: one is culture identification method, including blood bacterial culture and biochemical reaction and a series of processes, which usually takes 3-5 days. In recent years, the full-automatic blood culture and detection instrument developed to different degrees shortens the detection time and simplifies the operation steps. However, because the culture time of bacteria cannot be shortened indefinitely, it is determined that the detection method based on bacterial reproduction cannot finally overcome the defect of long time consumption no matter what instrument is used, which seriously restricts its application and development in clinical detection. The other is the rapid detection method represented by immunological method, such as antiserum agglutination test, synergistic agglutination test, enzyme-linked immunoassay and the like. However, these methods have the problems of low sensitivity and poor specificity, which often lead to misdiagnosis and missed diagnosis, and cannot meet the needs of clinical blood infection diagnosis. The molecular diagnosis method detects according to the characteristics of bacterial nucleic acid, which is the research hotspot of rapid detection method of clinical microorganism at present, and shows great development potential and application value.

[0003] In the implementation process of the prior art scheme, at least the following technical problems exist: special large instruments are needed, the operation is complicated, the technical difficulty is great, and the like, which are difficult to popularize and apply in clinic. The hybridization background of gene chip technology is high and difficult to eliminate, and has the problems of high false positive rate, great technical difficulty and high cost. Therefore, it is urgent to provide a blood infection pathogen rapid diagnosis device. SUMMARY

[0004] In view of the above technical problems, the present disclosure provides a blood infection pathogen rapid diagnosis device, which solves the technical problems in the prior art that special large instruments are needed, the operation is complicated, the technical difficulty is great, and the like, which are difficult to popularize and apply in clinic. The hybridization background of gene chip technology is high and difficult to eliminate, and has the problems of high false positive rate, great technical difficulty and high cost.

[0005] According to one aspect of the present disclosure, a blood infection pathogen rapid diagnosis device is provided, comprising a piezoelectric gene sensor, the piezoelectric gene sensor comprising a detection microarray connected to an oscillation circuit, a frequency counting module, a shockproof module, and a temperature control module; the detection microarray comprises a detection substrate, electrodes are arranged on the detection substrate, and a plurality of piezoelectric sensing units are arranged in an array on the detection substrate; the piezoelectric sensing unit comprises a base, an electrode socket is arranged at the bottom of the base to connect the electrodes on the detection substrate, the diameter of the electrode socket is not less than the diameter of the electrodes, a cylindrical detection hole is formed above the base, a quartz crystal oscillator is mounted at the bottom of the cylindrical detection hole, the quartz crystal oscillator is coated with a layer of gold film on both sides, and a probe is mounted on the surface of the quartz crystal oscillator in the cylindrical detection hole.

[0006] In some embodiments of the present disclosure, the oscillation circuit is a transistor oscillation circuit or a TTL oscillation circuit to drive the quartz crystal oscillator to stably oscillate at the resonant frequency.

[0007] In some embodiments of the present disclosure, the frequency counting module comprises an FPGA measurement element.

[0008] In some embodiments of the present disclosure, the shockproof module comprises an electromagnetic shield to avoid electromagnetic interference.

[0009] In some embodiments of the present disclosure, the temperature control module comprises a PID temperature automatic regulator.

[0010] In some embodiments of the present disclosure, the probe comprises an oligonucleotide probe.

[0011] In some embodiments of the present disclosure, the probe has a length of 18-26 bases.

[0012] The present utility model has the beneficial effects that:

[0013] The whole process from sample introduction to result output can be completed in a short time, greatly shortening the waiting time, and helping to take timely treatment measures. The use of oligonucleotide probes can specifically recognize and bind to the DNA or RNA sequence of the target pathogen, ensuring high sensitivity, specificity and accuracy of the detection. The design of the detection microarray allows multiple piezoelectric sensing units to be installed on the same platform, enabling simultaneous detection of pathogen samples and normal samples, simplifying the workflow as a control, reducing batch-to-batch differences caused by experimental condition fluctuations, ensuring data reliability, and the shock module includes an electromagnetic shield, effectively avoiding external electromagnetic interference and ensuring the stability of the measurement data. The use of a PID temperature automatic regulator maintains a constant working temperature, improves the chemical reaction rate, and makes the detection result have repeatability. The frequency counting module integrates the FPGA measurement element, not only improves the accuracy of frequency measurement, but also enhances the data processing speed and efficiency. The blood infection pathogen rapid diagnosis device provided by the utility model not only has the characteristics of rapidity, accuracy and high efficiency, but also has good portability and adaptability, can significantly improve the speed and quality of clinical diagnosis, and plays an important role in emergency situations or resource-poor environments. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a structural schematic view of the blood infection pathogen rapid diagnosis device;

[0015] Figure 2 It is a top view of the blood infection pathogen rapid diagnosis device;

[0016] Figure 3 It is Figure 2 A-A sectional view;

[0017] The names of various components in the figure are: 1, detection substrate;2, electrode;3, base;4, electrode socket;5, cylindrical detection hole;6, quartz crystal oscillator. DETAILED DESCRIPTION

[0018] The preferred embodiments of the utility model will be described below in conjunction with the drawings, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the utility model, and are not used to limit the utility model. Example 1

[0019] This example discloses a blood infection pathogen rapid diagnosis device, referring to Figures 1 to 3; including a piezoelectric gene sensor, the piezoelectric gene sensor including a detection microarray connected to an oscillation circuit, a frequency counting module, a shockproof module, and a temperature control module; the detection microarray including a detection substrate 1, the detection substrate 1 being provided with electrodes 2, and the detection substrate 1 being provided with a plurality of piezoelectric sensing units arranged in an array; the piezoelectric sensing unit including a base 3, the base 3 being provided at the bottom with electrode insertion holes 4 for connecting the electrodes 2 on the detection substrate 1, the diameter of the electrode insertion holes 4 not being less than the diameter of the electrodes 2, the base 3 being provided at the top with a cylindrical detection hole 5, the cylindrical detection hole 5 being provided at the bottom with a quartz crystal oscillator 6, the quartz crystal oscillator 6 being provided with a gold film on both sides, and the quartz crystal oscillator 6 being provided with a probe inside the cylindrical detection hole 5.

[0020] The oscillation circuit is a transistor oscillation circuit or a TTL oscillation circuit for driving the quartz crystal oscillator to stably oscillate at the resonant frequency.

[0021] The frequency counting module includes an FPGA measurement element.

[0022] The shockproof module includes an electromagnetic shield to avoid electromagnetic interference.

[0023] The temperature control module includes a PID temperature automatic regulator.

[0024] The probe includes an oligonucleotide probe.

[0025] The probe has a length of 18-26 bases.

[0026] During operation, when the sample blood is introduced into the detection microarray, the pathogen possibly existing in the sample blood will specifically hybridize with the specific oligonucleotide probe. This combination will change the mass load of the quartz crystal oscillator, thereby causing the oscillation frequency to change. The change in frequency is transmitted to the frequency counting module through the oscillation circuit and converted into a corresponding digital signal. The FPGA measurement element is responsible for processing these signals, calculating the actual frequency offset value, and further inferring the presence of the pathogen and its concentration. The temperature control module maintains a stable 37℃ environment temperature, and the shockproof module ensures that external interference will not affect the measurement results.

[0027] The piezoelectric gene sensor is based on the principle that the quartz crystal oscillator as a substrate interacts with the surrounding environment during oscillation, and the corresponding detection signal is converted from the change of the environmental medium such as mass, viscoelasticity, rheological property and the like by the change of the acoustic impedance spectrum, frequency spectrum or phase of the high-frequency acoustic wave of the device. The piezoelectric quartz resonant measurement technology is very sensitive to the change of the mass load on the crystal surface and the change of the properties of the system in which the quartz crystal is located, such as the density, viscosity, conductivity and dielectric constant of the solution, and can accurately measure the slight change in the micro-reaction process and convert it into a frequency signal that can be quantitatively detected. The piezoelectric DNA detection immerses the piezoelectric crystal with fixed oligonucleotide probes into the solution of its complementary sequence, and finds that the hybridization of the oligonucleotide fixed on the surface of the piezoelectric quartz crystal with the target nucleotide will cause a change in the oscillation frequency of the crystal, so that the amount of the target nucleotide is determined according to the change in the oscillation frequency of the crystal before and after hybridization. The self-assembled film method is to form a monolayer film of thiol-modified DNA molecules on the electrode surface by using the principle of monolayer technology. The sensitive film obtained by this method has the characteristics similar to biological membranes, and the surface structure is highly ordered. The monolayer technology refers to the thermodynamic stability and energy minimum ordered film formed by the self-adsorption of molecules on the solid / liquid or gas / solid interface through chemical bond interaction. The thiol compound can form a S-M bond on the metal surface such as gold electrode, which can ensure the selectivity of the combination, and the organic chain extending to the solution will form an ordered monolayer structure. Therefore, the self-assembled film has the characteristics similar to biological membranes in structure and function. By using this principle, the thiol-modified DNA forms a monolayer film on the gold electrode surface by automatically forming a chemical bond with the electrode.

[0028] Although some preferred embodiments of the present application have been described, those skilled in the art, once they know the basic creative concept, can make additional changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications falling within the scope of the present application.

[0029] Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application also intends to include these modifications and variations.

Claims

1. A rapid diagnostic device for bloodborne pathogens, characterized in that: The invention includes a piezoelectric gene sensor, comprising a detection microarray connected to an oscillation circuit, a frequency counting module, a shockproof module, and a temperature control module. The detection microarray includes a detection substrate with electrodes disposed thereon, and a plurality of piezoelectric sensing units arrayed on the substrate. Each piezoelectric sensing unit includes a base with electrode insertion holes at its bottom for connecting to the electrodes on the detection substrate. The diameter of the electrode insertion holes is not less than the diameter of the electrodes. A cylindrical detection hole is formed above the base, and a quartz crystal oscillator is mounted at the bottom of the cylindrical detection hole. Both sides of the quartz crystal oscillator are plated with a gold film, and a probe is mounted on the surface of the quartz crystal oscillator within the cylindrical detection hole.

2. The rapid diagnostic device for bloodborne pathogens as described in claim 1, characterized in that: The oscillation circuit is a transistor oscillation circuit or a TTL oscillation circuit, used to drive the quartz crystal oscillator to oscillate stably at the resonant frequency.

3. The rapid diagnostic device for bloodborne pathogens as described in claim 1, characterized in that: The frequency counting module includes FPGA measurement elements.

4. The rapid diagnostic device for bloodborne pathogens as described in claim 1, characterized in that: The shockproof module includes an electromagnetic shield to prevent electromagnetic interference.

5. The rapid diagnostic device for bloodborne pathogens as described in claim 1, characterized in that: The temperature control module includes a PID automatic temperature regulator.

6. The rapid diagnostic device for bloodborne pathogens as described in claim 1, characterized in that: The probes include oligonucleotide probes.

7. The rapid diagnostic device for bloodborne pathogens as described in claim 1, characterized in that: The probe is 18 to 26 bases in length.

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