Tool kit for dermatological experiment
By designing a slide collection basket and experimental plate device suitable for dermatopathology experiments, the problems of cumbersome operation and specimen damage caused by traditional tools have been solved, realizing an efficient and safe dermatopathology experimental process.
Patent Information
- Application Number
- CN202422557404.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-22
- Publication Date
- 2026-01-23
- Estimated Expiration
- 2034-10-22
AI Technical Summary
Traditional dermatopathology laboratory tools are cumbersome to operate, time-consuming, and prone to damaging specimens, and there is a risk of specimen omission and cross-contamination.
A tool kit including a slide collection basket and a test plate device was designed. The slide collection basket consists of a filter basket, a sealing cap and a handle. The test plate device consists of a porous reagent plate and a fixing groove. The filter screen corresponds to the holes of the reagent plate to prevent tissue damage and cross-contamination.
It improves experimental efficiency, prevents tissue damage and cross-contamination, and ensures specimen integrity.
Smart Images
Figure CN223827399U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a tool kit for dermatopathology experiments, belonging to the technical field of auxiliary tools for pathological experiments. Background Technology
[0002] Cutaneous nerve biopsy, or cutaneous nerve biopsy for short, is a morphological observation and quantitative study of intradermal nerve fibers (IENFD) in a patient. It involves detecting the density of intradermal nerve fibers (IENFD) by labeling unmyelinated axons in the epidermis using PGP 9.5 immunohistochemistry. The steps of skin pathology examination are as follows:
[0003] 1. Selecting a skin sample: The doctor will select a representative skin sample, usually through a biopsy.
[0004] 2. Processing skin samples: The specimens obtained from tissue biopsies are fully fixed with Zamboni fixative and then preserved with cryoprotectant. They are then cut into thin slices of about 50 μm using a skin slide machine and then stained.
[0005] 3. Sample observation: The stained skin sample will be placed under a microscope, and the doctor will analyze the density of nerve fibers in the epidermis and record any abnormalities.
[0006] 4. Analysis Results: The doctor will conduct a pathological analysis of the skin sample results and develop a treatment plan.
[0007] For a long time, the experimental tools used in dermatopathology experiments have been traditional, using a loop, similar to an egg retrieval tool, which requires repeatedly retrieving dermatopathology slides for the next step, resulting in nearly half of the experimental time being spent on slide retrieval.
[0008] Therefore, traditional tools are cumbersome to operate, take too long, and can damage specimens during experiments. Furthermore, accidental folding or omission can lead to specimen loss. Summary of the Invention
[0009] The purpose of this invention is to address the shortcomings of existing technologies by providing a tool kit for dermatopathology experiments.
[0010] To achieve the above objectives, this utility model provides a tool kit for dermatopathology experiments, characterized in that it includes a slide retrieval basket for collecting dermatopathology slides and an experimental plate device for the staining step; wherein:
[0011] The filter basket includes a filter basket, a sealing cover installed on the filter basket, and a handle. The filter basket includes a filter screen fixing frame and a filter screen installed in the filter screen fixing frame.
[0012] The experimental plate device includes a porous reagent plate for holding staining reagents. The porous reagent plate is provided with a plurality of cylindrical holes. The size, number and arrangement of the cylindrical holes on the porous reagent plate correspond to the size, number and arrangement of the filter holes at the bottom of the filter screen, so that the filter screen can be inserted into the cylindrical holes.
[0013] Preferably, the filter fixing frame is cylindrical.
[0014] Preferably, the experimental plate device further includes a fixing groove, in which the porous reagent plate is installed.
[0015] Preferably, the fixing groove is a cuboid structure without a cover.
[0016] Preferably, the filter screen has a pore size of 120 mesh.
[0017] Compared with the prior art, the beneficial effects of this utility model are as follows:
[0018] 1. It can greatly improve experimental efficiency in a convenient and quick manner.
[0019] 2. It can effectively prevent tissue damage during experimental shaking.
[0020] 3. Prevent tissue from being folded or missed when retrieving individual slices.
[0021] 4. Prevent cross-contamination during the experiment. Attached Figure Description
[0022] Figure 1 A schematic diagram of the structure of the slice-retrieving basket in the tool kit provided by this utility model;
[0023] Figure 2 A schematic diagram of the experimental board device in the tool kit provided by this utility model;
[0024] Figure reference numerals: 1. Slide pick-up basket; 2. Experimental plate apparatus; 11. Filter basket; 12. Sealing cap; 13. Handle; 14. Filter screen fixing frame; 21. Porous reagent plate; 22. Fixing groove; 23. Cylindrical hole. Detailed Implementation
[0025] To make this utility model more apparent and understandable, preferred embodiments are described in detail below with reference to the accompanying drawings.
[0026] Example 1
[0027] A tool kit for dermatopathology experiments, such as Figures 1-2 As shown, it includes a retrieval basket 1 for collecting skin pathology sections and an experimental plate device 2 for the staining step.
[0028] Among them, the slicing basket 1 is as follows Figure 1 As shown, the system includes a filter basket 11, a sealing cap 12, and a handle 13. The sealing cap 12 is mounted on the filter basket 11, and the handle 13 is mounted on the sealing cap 12. The filter basket 11 is a cylindrical hollow filter basket, which includes a cylindrical filter screen fixing frame 14 and a filter screen 15. The filter screen 15 is installed inside the cylindrical filter screen fixing frame 14. The cylindrical filter screen fixing frame 14 is made of plastic, and the filter screen 15 is made of nylon. The filter screen pore size should be set to ensure that the staining liquid can pass through but the skin sample cannot. Therefore, the filter screen pore size can be set to 120 mesh. The cylindrical filter screen fixing frame can effectively prevent tissue damage within the mold during subsequent staining experiments by reducing the damage to the tissue caused by the shaking force. The filter screen can effectively filter the liquid, preserving the tissue to the maximum extent until the next staining step. The sealing cap 12 can minimize the exposure of tissue sections to the external environment and avoid exogenous contamination. The handle 13 makes it easy to carry the sample to the next staining step. The sealing cap 12 and handle 13 can be made of the same material as the cylindrical filter screen fixing frame 14.
[0029] Experimental plate device 2 as follows Figure 2 As shown, it includes a porous reagent plate 21 (generally 23-well) for holding staining reagents and a fixing groove 22. The fixing groove 22 is an open cuboid structure. The porous reagent plate 21 is installed in the fixing groove 22, which can increase the stability of the porous reagent plate 21. The porous reagent plate 21 is provided with a number of cylindrical holes 23 (the cylindrical holes hold staining reagents during the staining experiment). The arrangement and number of the cylindrical holes 23 on the porous reagent plate 21 correspond to the holes at the bottom of the filter screen 15. During the staining step, the filter basket 11 of the slide collection basket 1 is placed into the experimental plate device 2, so that the filter screen 15 can be inserted into the cylindrical holes of the porous cylindrical reagent plate 2 and effectively fit against the porous cylindrical reagent plate 21, thereby allowing the skin tissue taken from the filter basket 11 to be completely immersed in the staining reagent. The entire experimental plate device 2 can be made of polystyrene.
[0030] The above tool kit is used in dermatopathology experiments as follows:
[0031] 1. First, place the staining reagent into the cylindrical well 23 of the porous reagent plate 21 according to the experimental procedure.
[0032] 2. Insert the retrieval basket 1 into the cylindrical hole 23.
[0033] 3. Place the skin sample into the sample collection basket 1.
[0034] 4. Insert the sample into each cylindrical hole 23 in sequence according to the experimental procedure. During the insertion process, the staining liquid can be filtered out so that the skin sample can still be in the filter of the slide collection basket 1.
[0035] The above-described embodiments are merely preferred embodiments of this utility model and are not intended to limit this utility model in any form or substance. It should be noted that those skilled in the art can make several improvements and additions without departing from this utility model, and these improvements and additions should also be considered within the scope of protection of this utility model.
Claims
1. A tool kit for dermatopathology experiments, characterized in that, Includes a retrieval basket for collecting dermatopathology slides and a laboratory plate apparatus for staining procedures; wherein: The filter basket includes a filter basket, a sealing cover installed on the filter basket, and a handle. The filter basket includes a filter screen fixing frame and a filter screen installed in the filter screen fixing frame. The experimental plate device includes a porous reagent plate for holding staining reagents. The porous reagent plate is provided with a plurality of cylindrical holes. The size, number and arrangement of the cylindrical holes on the porous reagent plate correspond to the size, number and arrangement of the filter holes at the bottom of the filter screen, so that the filter screen can be inserted into the cylindrical holes.
2. The tool kit as claimed in claim 1, characterized in that, The filter screen fixing frame is cylindrical.
3. The tool kit as described in claim 1, characterized in that, The experimental plate device also includes a fixing groove, in which the porous reagent plate is installed.
4. The tool kit as described in any one of claims 1 to 3, characterized in that, The filter screen has a pore size of 120 mesh.