Cell cryopreservation box matched with straw
By designing a rectangular cell cryopreservation box, the problems of small size and complicated operation of traditional cryopreservation boxes are solved. It enables large-capacity storage of wheat straws, simplifies operation, reduces costs and improves stability, and is suitable for cell preservation in liquid nitrogen environment.
Patent Information
- Application Number
- CN202520216776.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2035-02-12
AI Technical Summary
Traditional wheat straw cryopreservation boxes are too small, inconvenient to operate, require additional materials and are expensive, cannot hold wheat straws, and are not stable enough when stored in liquid nitrogen.
Design a rectangular cell cryopreservation box, comprising a rectangular base and a lid, with internal spacers and a liquid nitrogen inlet. The box is made of polypropylene or polyethylene. The lid and base are fitted together with an L-shaped protrusion to ensure a tight seal.
It enables large-capacity storage of wheat straw, simplifies the operation process, reduces costs, improves stability and space utilization, prevents liquid nitrogen leakage, and facilitates identification and rapid operation.
Smart Images
Figure CN223830251U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell storage and cryopreservation technology, specifically a cell cryopreservation box that is compatible with wheat straws. Background Technology
[0002] Wheat tube cell cryopreservation boxes are specialized containers used to store and protect cells or embryos. These cryopreservation boxes are designed to ensure that biological samples stored at extremely low temperatures (such as in liquid nitrogen) can maintain their viability and integrity for extended periods, unaffected by external environmental factors.
[0003] Wheat tubes are typically made of polypropylene or polyethylene and are suitable for cryopreserving embryos and sperm in liquid nitrogen. IMV brand wheat tubes are measured to be 13.3 cm long. After the sample is properly preserved and sealed, the wheat tube is inserted into a round thumb tube, secured to a cryogenic storage aluminum strip, and then placed in a matching liquid nitrogen container for immersion in liquid nitrogen for cryopreservation. In recent years, with the expansion of embryo and sperm / embryo bank resources using liquid nitrogen cryopreservation in wheat tubes, the practice of wheat tube preservation has become increasingly common.
[0004] However, the above methods have problems such as requiring a lot of materials (additional round thumb tubes, low-temperature storage aluminum strips, and special liquid nitrogen tanks are needed), making operation inconvenient and costly. Furthermore, while traditional cell cryopreservation boxes can be placed in liquid nitrogen, their small size makes it impossible to place wheat straws, further complicating operation. Utility Model Content
[0005] The purpose of this invention is to provide a cell cryopreservation box for wheat tubes to solve the problems mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution:
[0007] A cell cryopreservation box for wheat straws includes a cell cryopreservation box, which includes a base box and a lid. Both the base box and the lid are rectangular in structure, and the lid is fitted onto the base box.
[0008] The bottom box has several spacers evenly arranged inside to separate the straws. The bottom box has multiple liquid nitrogen inlet slots running through its center. The bottom box has protrusions on its outer wall near the top corners. The protrusions are L-shaped. When the lid is fastened onto the bottom box, the bottom of the lid abuts against the top of the protrusions.
[0009] As a preferred embodiment of this utility model, the cell cryopreservation box is 140*140*55mm, where the height of 55mm is the height of the box after the lid and the bottom box are fastened together.
[0010] As a preferred embodiment of this utility model, the bottom box has dimensions of 14cm*14cm*3.5cm and a thickness of 0.1cm.
[0011] As a preferred embodiment of this utility model, the liquid nitrogen inlet tank is 1cm*0.1cm in size.
[0012] As a preferred embodiment of this utility model, the spacer column is 0.5cm*0.5cm*3.0cm, and the center point of every two spacer columns is 1.4cm apart.
[0013] As a preferred embodiment of this invention, the cell cryopreservation box is made of polypropylene or polyethylene.
[0014] As a preferred embodiment of this utility model, the box lid has dimensions of 14.2cm*14.2cm*2.5cm and a thickness of 0.1cm.
[0015] Compared with the prior art, the beneficial effects of this utility model are:
[0016] 1. In response to the problems raised in the background art, the cell cryopreservation box of this application is enlarged to 140*140*55mm, so as to preserve wheat tubes and store a large number of wheat tubes, so that sealed wheat tubes can be put in, achieving the purpose of convenient placement of wheat tubes, convenient observation, and convenient operation.
[0017] 2. By using a rectangular bottom box and lid, as well as evenly spaced internal spacers and a liquid nitrogen inlet, the wheat tube can be easily placed in and its stability maintained, while ensuring that the liquid nitrogen can fully enter and contact the sample inside the wheat tube.
[0018] 3. The operation process is simplified, eliminating the need for additional materials such as round thumb tubes, low-temperature storage aluminum strips, and special liquid nitrogen tanks, thus reducing the difficulty and cost of operation;
[0019] 4. Improved space utilization: The rectangular structure design allows the cell cryopreservation boxes to be arranged more compactly inside the liquid nitrogen tank, thus improving space utilization.
[0020] 5. Enhanced stability: The evenly spaced internal spacers ensure the stability of the wheat tubes in liquid nitrogen, preventing sample damage caused by shaking.
[0021] 6. Optimized sealing performance: The matching snap-fit design between the lid and the bottom box, as well as the setting of protrusions, can effectively prevent liquid nitrogen leakage and ensure the safety of the samples.
[0022] 7. The operation is convenient and quick; simply open the cell cryopreservation box lid and insert the wheat tubes.
[0023] 8. Improved recognizability: The cell box has a large outer surface area, so larger labels can be attached to improve recognizability.
[0024] 9. Saves operation time. Through practice, it has been found that the entire operation of opening the liquid nitrogen tank, taking out the cell cryopreservation box and opening the lid, placing the straw, closing the cell cryopreservation box lid, placing it into the liquid nitrogen tank and closing the liquid nitrogen tank lid can be completed in 5-10 seconds.
[0025] 10. The cell cryopreservation box for wheat tubes used in this application has advantages over traditional wheat tube preservation, such as ease of operation, time and cost savings, and the ability to preserve cell cryopreservation tubes.
[0026] The present invention will be explained in detail below with reference to the accompanying drawings and specific embodiments. Attached Figure Description
[0027] Figure 1 This is a side view of the cell cryopreservation box of this utility model.
[0028] Figure 2 This is a top view of the base box of this utility model.
[0029] Figure 3 This is a side view of the bottom box of this utility model.
[0030] Figure 4 This is a top view of the box lid of this utility model.
[0031] In the diagram: 1. Cell cryopreservation box; 11. Base box; 111. Spacer column; 112. Liquid nitrogen inlet tank; 113. Protrusion; 12. Box lid. Detailed Implementation
[0032] To facilitate understanding of this utility model, a more comprehensive description of the utility model will be given below with reference to the accompanying drawings, which show several embodiments of the utility model. However, the utility model can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the disclosure of the utility model more thorough and comprehensive.
[0033] Example
[0034] Please see Figure 1-4This utility model provides a technical solution: a cell cryopreservation box for use with wheat straws, comprising a cell cryopreservation box 1, which includes a base box 11 and a lid 12. Both the base box 11 and the lid 12 are rectangular in shape, and the lid 12 is fitted onto the base box 11. The cell cryopreservation box 1 measures 140*140*55mm, where the height of 55mm is the height of the lid 12 and the base box 11 when fitted together. The base box 11 measures 14cm*14cm*3.5cm and has a thickness of 0.1cm. The material is polypropylene or polyethylene. The lid 12 is 14.2cm*14.2cm*2.5cm and 0.1cm thick. The bottom box 11 has several spacers 111 evenly arranged inside to separate the straws. The spacers 111 are 0.5cm*0.5cm*3.0cm and the center point of every two spacers 111 is 1.4cm apart. The bottom box 11 has multiple liquid nitrogen inlet tanks 112 through the middle for liquid nitrogen entry. The liquid nitrogen inlet tanks 112 are 1cm*0.1cm. The bottom box 11 has protrusions 113 on the outer wall near the top corners. The protrusions 113 are arranged in an L-shape. When the lid 12 is fastened on the bottom box 11, the bottom of the lid 12 abuts against the top of the protrusions 113.
[0035] It should be noted that, in this embodiment, the overall dimensions of the cell cryopreservation box are 140mm x 140mm x 55mm, providing sufficient space to accommodate multiple wheat tubes. The dimensions of the bottom box are 140mm x 140mm x 35mm, and the dimensions of the lid are 142mm x 142mm x 25mm, ensuring that the lid 12 can be tightly fastened to the bottom box 11. The material is polypropylene or polyethylene, which have good low-temperature resistance and are suitable for use in liquid nitrogen environments.
[0036] Furthermore, the bottom box is evenly equipped with several (9 rows and 9 columns, a total of 81) spacers 111. Each spacer 111 measures 0.5cm x 0.5cm x 3.0cm, and the center point spacing of the spacers 111 is 1.4cm. This design can effectively separate each straw and prevent them from colliding or sticking together in liquid nitrogen.
[0037] Furthermore, multiple liquid nitrogen inlet slots 112 are provided through the center of the bottom box 11, each slot measuring 1cm x 0.1cm. These slots allow liquid nitrogen to enter from the bottom, ensuring that the liquid nitrogen around the straw can circulate fully, thereby maintaining the sample at a low temperature.
[0038] Furthermore, L-shaped protrusions 113 are provided on the outer wall near the top corners of the bottom box 11. When the lid 12 is fastened onto the bottom box 11, the bottom of the lid 12 abuts against the top of the protrusions 113. This design enhances the sealing between the lid 12 and the bottom box 11, preventing liquid nitrogen leakage.
[0039] Furthermore, the design simplifies the operation process, eliminating the need for additional materials such as circular thumb tubes, cryogenic storage aluminum strips, and dedicated liquid nitrogen tanks;
[0040] The rectangular structure improves space utilization, allowing the cell cryopreservation box 1 to be arranged more compactly inside the liquid nitrogen tank;
[0041] Enhanced stability; the uniformly spaced internal spacers 111 ensure the stability of the wheat tube in liquid nitrogen.
[0042] The sealing performance has been optimized. The matching and snapping design between the lid 12 and the bottom box 11, as well as the setting of the protrusion 113, effectively prevent liquid nitrogen leakage.
[0043] This cell cryopreservation box design with matching wheat tubes has significant advantages in terms of practicality, efficiency, and safety, making it an ideal choice for long-term preservation of biological samples.
[0044] The working process of this utility model:
[0045] When using this method, fill the prepared cell samples into straws, ensuring that the sample volume in each straw is appropriate, neither too full nor too little. Open the liquid nitrogen tank, take out the cell cryopreservation box 1 and open the lid 12, place the straws in the box, arrange them according to the designed layout, ensuring that there is enough space between each straw. Close the lid 12 of the cell cryopreservation box, put it into the liquid nitrogen tank and close the lid of the liquid nitrogen tank. The entire operation is convenient and quick. You can put the straws in by simply opening the lid 12 of the cell cryopreservation box, saving operation time.
[0046] When a sample needs to be retrieved, simply open the lid 12 and take out the required straw. The evenly spaced columns 111 inside make it easy to find the required straw. After use, clean and sterilize the cryopreservation box for the next use.
[0047] The present invention has been described above by way of example in conjunction with the accompanying drawings. Obviously, the specific implementation of the present invention is not limited to the above-described manner. Any non-substantial improvement made by adopting the inventive concept and technical solution of the present invention, or the direct application of the inventive concept and technical solution of the present invention to other occasions without modification, shall be within the protection scope of the present invention.
Claims
1. A cell cryopreservation box for use with wheat straws, comprising a cell cryopreservation box (1), characterized in that: The cell cryopreservation box (1) includes a base box (11) and a lid (12). Both the base box (11) and the lid (12) are rectangular in shape, and the lid (12) is fitted onto the base box (11). The bottom box (11) is evenly provided with a number of spacer columns (111) for separating wheat straws. The bottom box (11) is provided with a number of liquid nitrogen inlet slots (112) for liquid nitrogen to enter through the middle. The bottom box (11) is provided with protrusions (113) on the outer wall near the top corners. The protrusions (113) are arranged in an L-shaped structure. When the box cover (12) is fastened on the bottom box (11), the bottom of the box cover (12) abuts against the top of the protrusions (113).
2. The cell cryopreservation box with matching wheat tubes according to claim 1, characterized in that: The cell cryopreservation box (1) is 140*140*55mm, where the height of 55mm is the height of the lid (12) and the bottom box (11) after they are fastened together.
3. The cell cryopreservation box with matching wheat tubes according to claim 1, characterized in that: The bottom box (11) is 14cm*14cm*3.5cm and 0.1cm thick.
4. The cell cryopreservation box with matching wheat tubes according to claim 1, characterized in that: The liquid nitrogen inlet tank (112) is 1cm*0.1cm.
5. A cell cryopreservation box for matching wheat tubes according to claim 1, characterized in that: The spacer column (111) is 0.5cm*0.5cm*3.0cm, and the center point of every two spacer columns (111) is 1.4cm apart.
6. The cell cryopreservation box with matching wheat tubes according to claim 1, characterized in that: The cell cryopreservation box (1) is made of polypropylene or polyethylene.
7. A cell cryopreservation box for matching wheat tubes according to claim 1, characterized in that: The box lid (12) measures 14.2cm*14.2cm*2.5cm and is 0.1cm thick.