Detachable glass bottom cell device

By designing a detachable glass-bottom cell apparatus, the problem of not being able to observe cell details under an oil immersion microscope in plastic six-well plates was solved, enabling efficient cell culture and microscopic observation, reducing operational complexity and contamination risks, and improving experimental efficiency and result reliability.

CN223837439UActive Publication Date: 2026-01-27HUADU DISTRICT GUANGZHOU CITY PEOPLES HOSPITAL
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Patent Information

Application Number
CN202520215794.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-02-11
Publication Date
2026-01-27
Estimated Expiration
2035-02-11

AI Technical Summary

Technical Problem

The existing six-well plates are made of plastic, which makes it impossible to observe cell details through an oil immersion microscope. They need to be transferred to glass-bottomed culture dishes, which is cumbersome and increases the risk of contamination.

Method used

Design a detachable glass-bottom cell apparatus, including a glass-bottom culture dish, an inner cover, a plastic culture plate, and an outer cover. The plastic culture plate has a receiving groove inside. The glass-bottom culture dish is detachable for easy cleaning and combination. The glass-bottom culture dish has high refractive index and is suitable for oil immersion observation.

Benefits of technology

It improves experimental efficiency, reduces the risk of cell contamination, enables high-throughput operation and microscopic observation, and features a detachable device for easy cleaning and a well-sealed design to ensure the reliability of experimental results.

✦ Generated by Eureka AI based on patent content.

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Abstract

A detachable glass bottom cell device comprises a glass bottom culture dish, an inner cover matched with the glass bottom culture dish, a plastic culture plate and an outer cover matched with the plastic culture plate, a plurality of containing grooves are formed in the plastic culture plate, the glass bottom culture dish is arranged in the containing grooves, and a handle is arranged on the outer wall of the glass bottom culture dish. The principle of the utility model is as follows: the plastic culture bottom is used as a carrier of the culture dish, a plurality of culture dishes with glass bottoms can be intensively used for cell culture, and the experiment efficiency is improved; the glass bottom culture dish and the plastic culture bottom can be separated from each other and are convenient to clean and combine for use; the culture dish with the glass bottom has high diopter, cell details can be directly observed by using an oil lens, cell transfer is not needed, the efficiency is high, and the risk that the cells are polluted is reduced.
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Description

Technical Field

[0001] This utility model relates to the fields of medical and biological technology, and in particular to a detachable glass-bottom cell device. Background Technology

[0002] Six-well plates are commonly used cell culture devices. Made of polystyrene, they contain six independent wells, each approximately 35 mm in diameter and 17 mm deep, with an overall size of approximately 127.76 mm x 85.47 mm. Six-well plates are essential tools for cell culture, offering sterility and transparency, making them suitable for various biological experiments. While cell status can be monitored using a microscope or microplate reader, the plastic material prevents detailed observation through an oil immersion lens. Cells must be transferred to a glass-bottomed culture dish with a higher refractive index for observation, which complicates experimental procedures. Summary of the Invention

[0003] The technical problem to be solved by this utility model is to provide a detachable glass-bottom cell device that is simple to operate and convenient for cleaning and cell observation.

[0004] To solve the above-mentioned technical problems, the technical solution of this utility model is: a detachable glass-bottom cell culture device, comprising a glass-bottom culture dish, an inner cover that mates with the glass-bottom culture dish, a plastic culture plate, and an outer cover that mates with the plastic culture plate. The plastic culture plate has several receiving slots, and the glass-bottom culture dish is placed within these slots. A handle is provided on the outer wall of the glass-bottom culture dish. The principle of this utility model is as follows: the plastic culture base serves as a carrier for the culture dish, allowing multiple glass-bottom culture dishes to be cultured together, improving experimental efficiency; the glass-bottom culture dish and the plastic culture base can be separated for easy cleaning and combination; the glass-bottom culture dish has high refractive index, allowing direct observation of cell details using an oil immersion microscope without the need for cell transfer, resulting in high efficiency and reducing the risk of cell contamination.

[0005] As an improvement, the plastic culture plate includes a rectangular base plate, side panels arranged along the edge of the base plate, and partitions disposed within the side panels, wherein the receiving groove is formed by the partitions.

[0006] As an improvement, a total of six receiving slots are provided, which are arranged in an array of two rows and three columns.

[0007] As an improvement, the glass-bottomed culture dish is circular, the receiving groove is square, and the side panel is provided with a notch for the handle to extend out.

[0008] As an improvement, the inner wall of the receiving groove is provided with limiting ribs.

[0009] The beneficial effects of this utility model compared with the prior art are:

[0010] 1. High-throughput operation: The plastic culture plate has multiple accommodating slots, which can culture multiple samples simultaneously, improving experimental efficiency;

[0011] 2. Facilitates microscopic observation: The bottom of the glass-bottomed petri dish has good light transmittance, making it suitable for observation under a high-resolution microscope;

[0012] 3. Easy disassembly: The glass-bottomed petri dish is equipped with a handle for easy handling; the receiving groove is equipped with limiting ribs to ensure stable placement of the petri dish;

[0013] 4. Reusable: The device is detachable, making it easy to clean and disinfect, thus reducing experimental costs;

[0014] 5. Excellent sealing: The inner and outer caps are designed to effectively prevent contamination and ensure the reliability of experimental results. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the present invention. Detailed Implementation

[0016] The present invention will be further described below with reference to the accompanying drawings.

[0017] like Figure 1 As shown, a detachable glass-bottomed cell culture device includes a glass-bottomed culture dish 2, an inner cover 3 that mates with the glass-bottomed culture dish 2, a plastic culture plate 1, and an outer cover 4 that mates with the plastic culture plate 1. The plastic culture plate 1 has several receiving slots 11. The plastic culture plate 1 includes a rectangular base plate, side panels along the edge of the base plate, and partitions within the side panels. The receiving slots 11 are formed by the partitions. In this embodiment, there are six receiving slots 11 arranged in a two-row, three-column array. The glass-bottomed culture dish 2 is circular, and the receiving slots 11 are square. The glass-bottomed culture dish 2 is placed within the receiving slots 11. The inner wall of the receiving slots 11 has limiting ribs to ensure stable placement of the culture dish. The outer wall of the glass-bottomed culture dish 2 has a handle 21, and the side panels have notches 12 for the handle 21 to extend out, facilitating the placement and removal of the culture dish.

[0018] The principle of this invention is as follows: the plastic culture base serves as a carrier for the culture dish, allowing multiple glass-bottomed culture dishes 2 to be cultured together, thus improving experimental efficiency; the glass-bottomed culture dish 2 and the plastic culture base can be separated from each other, making them easy to clean and combine; the glass-bottomed culture dish 2 has a high refractive index, allowing direct observation of cell details using an oil immersion lens without the need for cell transfer, resulting in high efficiency and reducing the risk of cell contamination.

Claims

1. A detachable glass-bottom cell device, characterized in that: The invention includes a glass-bottomed culture dish, an inner cover that fits with the glass-bottomed culture dish, a plastic culture plate, and an outer cover that fits with the plastic culture plate. The plastic culture plate has several receiving slots, the glass-bottomed culture dish is placed in the receiving slots, and the outer wall of the glass-bottomed culture dish has a handle.

2. The detachable glass-bottom cell device according to claim 1, characterized in that: The plastic culture plate includes a rectangular base plate, side panels along the edge of the base plate, and partitions within the side panels. The receiving groove is formed by the partitions.

3. The detachable glass-bottom cell device according to claim 2, characterized in that: There are a total of six receiving slots, which are arranged in an array of two rows and three columns.

4. The detachable glass-bottom cell device according to claim 2, characterized in that: The glass-bottomed culture dish is circular, the receiving groove is square, and the side panel has a notch for the handle to extend out.

5. A detachable glass-bottom cell device according to claim 1, characterized in that: The inner wall of the receiving groove is provided with limiting ribs.