Nanochannel glucose colorimetric detection device based on enzyme immobilization

Abstract

The utility model provides a nanochannel glucose colorimetric detection device based on enzyme immobilization, and belongs to the technical field of biosensors. Aiming at the defects in detection sensitivity, anti-interference performance and operation stability in the prior art, the device comprises a nano channel, a sample storage module, a colorimetric detection module, a fixed support, a gasket and a fixed suction cup clamping device, the sample storage module is provided with a sample cell, and the colorimetric detection module is provided with a detection cell and a colorimetric card; the fixed suction cup clamping device comprises a suction cup and a clamping device, the suction cup is fixedly connected with the clamping device, the suction cup is fixed to the right end face of the colorimetric detection module, and the clamping device is adjusted so that the sample storage module and the colorimetric detection module can clamp the nano channel through the gasket, and the sample storage module can be tightly attached to the fixed support. The glucose detection device disclosed by the utility model has the advantages of high sensitivity, strong anti-interference performance, simplicity and convenience in operation and the like, and can be widely applied to the fields of blood glucose monitoring, food detection, environmental analysis and the like of diabetic patients.

Description

Technical Field

[0001] This invention belongs to the field of biosensor technology, specifically relating to a glucose colorimetric detection device based on enzyme-immobilized nanochannels. Background Technology

[0002] Diabetes mellitus, a prevalent metabolic disease worldwide, requires patients to continuously monitor their blood glucose levels for effective management. However, existing blood glucose detection methods (such as electrochemical and optical detection) have limitations in practical applications, especially in complex samples (such as undiluted serum), exhibiting low sensitivity, poor stability, and insufficient resistance to interference. Furthermore, conventional electrochemical sensors, limited by material selection and detection method optimization, struggle to simultaneously achieve high sensitivity and reliability. Therefore, developing a highly sensitive, easy-to-operate glucose detection device that can effectively cope with interference from complex samples is of paramount importance. Utility Model Content

[0003] To address the shortcomings of existing technologies in terms of detection sensitivity, anti-interference, and operational stability, the purpose of this invention is to provide a glucose colorimetric detection device based on enzyme-immobilized nanochannels. This device can efficiently and stably detect glucose in serum, solving the problems of inaccurate detection and poor anti-interference in complex samples in existing technologies.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] This invention provides a glucose colorimetric detection device based on enzyme-immobilized nanochannels, comprising: a nanochannel, a sample storage module, a colorimetric detection module, a fixing support, a gasket, and a fixing suction cup clamping device; the sample storage module and the colorimetric detection module are placed in the fixing support, with the right end face of the sample storage module and the left end face of the colorimetric detection module facing each other, and the symmetrical central areas of the right end face of the sample storage module and the left end face of the colorimetric detection module are respectively fixedly connected to the gasket, and the gasket has a through hole in the center;

[0006] The sample storage module is equipped with a sample cell, and the colorimetric detection module is equipped with a detection cell and a colorimetric card. The colorimetric card is placed on top of the detection cell. The sample cell and the detection cell are respectively equipped with passages of the same diameter as the through holes of the gasket.

[0007] The fixed suction cup clamping device includes a suction cup and a clamping device. The suction cup is fixedly connected to the clamping device. The suction cup is fixed on the right end face of the colorimetric detection module. The clamping device is adjusted so that the sample storage module and the colorimetric detection module clamp the nanochannel through the gasket and make the sample storage module fit tightly against the fixed support.

[0008] Furthermore, the nanochannel includes a titanium dioxide nanobase layer with through-pores inside. The two ends of the through-pores have different diameters, namely a large end and a small end. The large end faces the sample storage module side, and the small end faces the colorimetric detection module side. The surface of the through-pores inside the nanochannel has a catalytic reaction layer and a colorimetric layer. The catalytic reaction layer is a metal-organic framework (MOFs) and glucose oxidase (GOD) composite structure grown in situ on the inner surface of the titanium dioxide nanochannel. The surface of the catalytic reaction layer is a colorimetric layer, which is a colorimetric agent adsorbed on the surface of the catalytic reaction layer.

[0009] Furthermore, the metal-organic framework is a ZIF-8 structure.

[0010] Furthermore, a pH-responsive gated structure layer is present on the side end face of the small opening of the titanium dioxide nanochannel.

[0011] Furthermore, the pH-responsive gated structure layer is a pH-responsive polymer layer formed by a metal-organic framework on the side end face of the small opening of the titanium dioxide nanochannel.

[0012] Furthermore, the pH-responsive polymer is poly(4-vinylpyridine) (P4VP).

[0013] Furthermore, the pore size of the internal pores of the nanochannels can be adjusted according to the sample requirements.

[0014] Furthermore, the sample cell pathway is connected to the large end of the nanochannel via a gasket through-hole, and the detection cell pathway is connected to the small end of the nanochannel via a gasket through-hole.

[0015] Furthermore, the clamping device is a bolt, with the screw fixedly connected to the suction cup. A screw hole is provided on the fixed support, through which the screw passes through the screw hole and is clamped and adjusted by the nut outside the fixed support.

[0016] Furthermore, the gasket material is polydimethylsiloxane (PMDS).

[0017] Advantages and effects of this utility model:

[0018] This novel device is suitable for highly sensitive detection of glucose in complex matrices. It exhibits strong anti-interference capabilities in complex matrices such as undiluted serum, effectively eliminating interfering factors in samples and ensuring accurate detection. It is particularly suitable for real-time blood glucose monitoring in diabetic patients. Due to the stability of the nanochannel structure and the high efficiency of enzyme immobilization technology, it has the potential for long-term use and can be applied to medical testing equipment and home health management. The device has a simple structure and is easy to operate, making it suitable for rapid on-site use. It can be widely used for real-time blood glucose monitoring in diabetic patients and can be extended to food testing, environmental sample analysis, and other fields. With the continuous maturation of the technology, it is expected to have broad market demand and application prospects, especially playing an important role in health management and clinical diagnosis. Attached Figure Description

[0019] Figure 1 This is a front view of an enzyme-immobilized nanochannel glucose colorimetric detection device according to Embodiment 1 of this utility model;

[0020] Figure 2 This is a top view of an enzyme-immobilized nanochannel glucose colorimetric detection device according to Embodiment 1 of this utility model;

[0021] Figure 3 This is a schematic diagram of the titanium dioxide nanochannel structure in an enzyme-immobilized nanochannel glucose colorimetric detection device according to Embodiment 1 of this utility model.

[0022] Figure 4 This is a schematic diagram of the nanochannel structure in an enzyme-immobilized nanochannel glucose colorimetric detection device according to Embodiment 1 of this utility model;

[0023] Reference numerals: 1. Nanochannel; 1-01. Large opening of nanochannel; 1-02. Small opening of nanochannel; 2. Sample storage module; 2-01. Sample cell; 3. Colorimetric detection module; 3-01. Detection cell; 3-02. Colorimetric card; 4. Fixing support; 5. Gasket; 6. Fixing suction cup clamping device; 6-01. Suction cup; 6-02. Bolt. Detailed Implementation

[0024] The present invention will now be described in detail with reference to the embodiments.

[0025] Example 1

[0026] like Figure 1As shown, a glucose colorimetric detection device based on enzyme immobilization nanochannels includes a nanochannel 1, a sample storage module 2, a colorimetric detection module 3, a fixing support 4, a gasket 5, and a fixing suction cup clamping device 6. The sample storage module 2 and the colorimetric detection module 3 are placed in the fixing support 4, with the right end face of the sample storage module 2 and the left end face of the colorimetric detection module 3 facing each other. The central areas of the symmetrical right end face of the sample storage module 2 and the left end face of the colorimetric detection module 3 are respectively fixedly connected to the gasket 5. The gasket has a through hole in the center, and the gasket material is polydimethylsiloxane (PMDS).

[0027] like Figure 2 As shown, the sample storage module 2 is provided with a sample cell 2-01, and the colorimetric detection module 3 is provided with a detection cell 3-01 and a colorimetric card 3-02. The colorimetric card 3-02 is placed at the top of the detection cell 3-01. The sample cell 2-01 and the detection cell 3-01 are respectively provided with passages of the same diameter corresponding to the position of the gasket through hole.

[0028] like Figure 1 As shown, the fixed suction cup clamping device 6 includes a suction cup 6-01 and a clamping device bolt 6-02. The screw is fixedly connected to the suction cup 6-01. The fixed support 4 is provided with a screw hole. The screw passes through the screw hole through the fixed support 4 and is clamped and adjusted by the nut outside the fixed support 4. The suction cup 6-01 is fixed on the right end face of the colorimetric detection module 3. The clamping device 6-02 is adjusted so that the sample storage module 2 and the colorimetric detection module 3 clamp the nanochannel 1 through the gasket 5 and make the sample storage module 2 fit tightly against the fixed support.

[0029] like Figure 3 , Figure 4 As shown, nanochannel 1 uses titanium dioxide nanochannel (TiNM) as its core substrate. The two end faces of the titanium dioxide nanochannel have different pore sizes, divided into a large-mouth end 1-01 and a small-mouth end 1-02. The large-mouth end 1-01 faces the sample storage module 2, and the small-mouth end 1-02 faces the colorimetric detection module 3. The surface of the internal through-pores of the nanochannel has a catalytic reaction layer and a colorimetric layer. The catalytic reaction layer is a composite structure GOD@ZIF-8 formed by in-situ growth and immobilization of glucose oxidase (GOD) within the titanium dioxide nanochannel using metal-organic frameworks (MOFs). The surface of the catalytic reaction layer is a colorimetric layer, which is a colorimetric agent adsorbed on the surface of the catalytic reaction layer. On the side end face 1-02 of the small-mouth end of the titanium dioxide nanochannel, there is a pH-responsive gating structure layer. The pH-responsive gating structure layer is a pH-responsive poly(4-vinylpyridine) (P4VP) layer formed by metal-organic frameworks on the side end face of the small-mouth end of the titanium dioxide nanochannel.

[0030] The passage of sample cell 2-01 is connected to the large end 1-01 of nanochannel through the through hole of gasket 5, and the passage of detection cell 3-01 is connected to the small end 1-02 of nanochannel through the through hole of gasket 5.

[0031] The detection principle is as follows:

[0032] The sample to be tested is placed in the sample cell and enters the nanochannel through the sample cell pathway and the gasket through-hole from the large opening end of the nanochannel. The nanochannel uses titanium dioxide nanochannels (TiNM) as the core substrate (TiNM is prepared on a titanium substrate by anodizing). The channel pore size can be adjusted according to the detection requirements of different samples. The TiNM structure is a mass transfer channel with high biocompatibility and stability, which can effectively improve the catalytic efficiency and stability of glucose oxidase. Glucose oxidase (GOD) is immobilized on the TiNM surface using a biomimetic mineralization method and then grown in situ within the TiNM channel using metal-organic frameworks (MOFs). To enhance its stability, a composite structure is formed to improve glucose conversion efficiency and detection sensitivity. In the sample to be tested, glucose undergoes a catalytic reaction through immobilized glucose oxidase (GOD) to generate gluconic acid and hydrogen peroxide, triggering a pH change. This triggers a pH-responsive gated structure layer on the side face of the small end of the TiNM. This gated structure layer is a pH-responsive polymer (such as P4VP) layer formed by a metal-organic framework. The chromogenic agent is released, and a color change occurs in the detection cell. The degree of color change is directly proportional to the glucose concentration. After the sample to be tested enters the detection cell, the glucose concentration is quantified by comparing the color of the sample cell with the colorimetric card.

Claims

1. A colorimetric glucose detection device based on enzyme-immobilized nanochannels, characterized in that, include: The sample storage module, colorimetric detection module, fixed support, gasket, and fixed suction cup clamping device are provided. The sample storage module and colorimetric detection module are placed in the fixed support, with the right end face of the sample storage module and the left end face of the colorimetric detection module facing each other. The central areas of the symmetrical right end face of the sample storage module and the left end face of the colorimetric detection module are fixedly connected to the gasket, and the gasket has a through hole in the center. The sample storage module is equipped with a sample cell, and the colorimetric detection module is equipped with a detection cell and a colorimetric card. The colorimetric card is placed on top of the detection cell. The sample cell and the detection cell are respectively equipped with passages of the same diameter as the through holes of the gasket. The fixed suction cup clamping device includes a suction cup and a clamping device. The suction cup is fixedly connected to the clamping device. The suction cup is fixed on the right end face of the colorimetric detection module. The clamping device is adjusted so that the sample storage module and the colorimetric detection module clamp the nanochannel through the gasket and make the sample storage module fit tightly against the fixed support.

2. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 1, characterized in that, The nanochannel comprises a titanium dioxide nanobase layer with through-pores inside. The two ends of the through-pores have different diameters, namely a large end and a small end. The large end faces the sample storage module, and the small end faces the colorimetric detection module. The surface of the through-pores inside the nanochannel has a catalytic reaction layer and a colorimetric layer. The catalytic reaction layer is a metal-organic framework and glucose oxidase composite structure grown in situ on the inner surface of the titanium dioxide nanochannel. The colorimetric layer is a colorimetric agent adsorbed on the surface of the catalytic reaction layer.

3. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 2, characterized in that, The metal-organic framework is a ZIF-8 structure.

4. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 2, characterized in that, A pH-responsive gated structure layer is present on the side end face of the nanochannel's small opening.

5. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 4, characterized in that, The pH-responsive gated structure layer is a pH-responsive polymer layer formed by a metal-organic framework on the side end face of the small opening of a titanium dioxide nanochannel.

6. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 5, characterized in that, The pH-responsive polymer is poly(4-vinylpyridine).

7. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 1, characterized in that, The pore size of the internal pores of the nanochannels can be adjusted according to the sample requirements.

8. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 1, characterized in that, The sample cell pathway is connected to the large end of the nanochannel through a gasket through-hole, and the detection cell pathway is connected to the small end of the nanochannel through a gasket through-hole.

9. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 1, characterized in that, The clamping device is a bolt, with the screw fixedly connected to the suction cup. The fixed support has a screw hole, through which the screw passes through the screw hole and is clamped and adjusted by the nut outside the fixed support.

10. The enzyme-immobilized nanochannel glucose colorimetric detection device as described in claim 1, characterized in that, The gasket material is polydimethylsiloxane.

Images