Electric ultrafiltration device for separating protein

Abstract

The utility model relates to the technical field of protein separation, in particular to an electric ultrafiltration device for separating protein. According to the electric ultrafiltration device for separating the protein, the functional chambers are arranged in the electrolytic bath, so that the functional chambers of the device can be mutually independent, and separation of different protein products is facilitated; the functional chamber comprises an anode chamber, a cathode chamber, a stock solution chamber and a dialysis chamber, an anode plate is mounted in the anode chamber, a cathode plate is mounted in the cathode chamber, an ultrafiltration membrane mechanism is mounted in the dialysis chamber, a first positive membrane is mounted in the stock solution chamber, and a second positive membrane is mounted in the dialysis chamber, so that membranes are convenient to clean and replace; meanwhile, the diaphragm can be recycled for a long time, the operation is simple, and the operation is stable.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein separation technical field, concretely relate to an electric ultrafiltration device for separating protein. BACKGROUND

[0002] Protein separation and purification is a challenging work, since protein exists in complex biological system, and is unstable, and is easy to denature and inactivate when meeting heat or some solvents, so traditional distillation, solvent extraction and other separation techniques are not suitable for its separation and purification, and chromatography is used for protein separation and purification, although it has the characteristics of high resolution, but the operation is complicated, difficult to enlarge, and the cost is high, and its application in industrial production is limited.

[0003] At present, the ultrafiltration membrane system has been very maturely applied to various industrial production processes. The mechanism of the ultrafiltration membrane is the comprehensive effect of mechanical screening on the membrane surface, membrane hole blockage and membrane surface and membrane hole adsorption, mainly for screening, for intercepting colloidal particles in the liquid, and water and low molecular weight solutes are allowed to pass through the membrane, the required pressure is small, the screening speed is slow, and the membrane pollution is relatively serious. But different electric properties and different molecular weight protein separation is difficult to meet the requirements of ultrafiltration equipment because of the concentration difference.

[0004] Therefore, it is urgent to provide an electric ultrafiltration device capable of inhibiting concentration polarization and membrane pollution, and further improving the separation and concentration efficiency. UTILITY MODEL CONTENT

[0005] The utility model aims at least to solve one of the technical problems existing in the prior art, and provides an electric ultrafiltration device for separating protein.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: an electric ultrafiltration device for separating protein, comprising an electrolytic cell, a plurality of function chambers are arranged in the electrolytic cell, the function chambers include an anode chamber, a cathode chamber, a raw liquid chamber and a dialysis chamber, an anode plate is installed in the anode chamber, a cathode plate is installed in the cathode chamber, an ultrafiltration membrane mechanism is installed in the dialysis chamber, a first anode membrane is installed in the raw liquid chamber, and a second anode membrane is installed in the dialysis chamber.

[0007] Further, a plurality of baffle gaskets are arranged in the electrolytic cell, and the baffle gaskets abut the inner wall of the electrolytic cell.

[0008] Further, the baffle gaskets include four, and the four baffle gaskets are located behind the anode plate, the first anode membrane, the second anode membrane and the ultrafiltration membrane mechanism respectively.

[0009] Further, the raw liquid chamber and the dialysis chamber are arranged between the anode chamber and the cathode chamber.

[0010] Further, the first anode membrane and the second anode membrane are symmetrically arranged in the electrolytic cell.

[0011] Further, the first anode membrane is arranged close to one side of the anode plate, and the second anode membrane is arranged close to one side of the cathode plate.

[0012] Further, the ultrafiltration membrane mechanism comprises an ultrafiltration membrane support plate installed in the dialysis chamber, and the ultrafiltration membrane is installed on the ultrafiltration membrane support plate.

[0013] Further, the ultrafiltration membrane is installed between the first anode membrane and the second anode membrane.

[0014] Compared with the prior art, the technical scheme of the present application has the following beneficial effects:

[0015] 1. The device has a plurality of function chambers in the electrolytic cell, so that the function chambers can be independent of each other, facilitating the separation of different protein products; the function chambers comprise an anode chamber, a cathode chamber, a raw liquid chamber and a dialysis chamber, the anode chamber is provided with an anode plate, the cathode chamber is provided with a cathode plate, the dialysis chamber is provided with an ultrafiltration membrane mechanism, the raw liquid chamber is provided with a first anode membrane, and the dialysis chamber is provided with a second anode membrane, so that the membrane can be cleaned and replaced, and the membrane can be used for a long time, which is simple in operation and stable in running.

[0016] 2. The device is simple to install and use, easy to maintain, made of stainless steel, corrosion-resistant, long service life, stable operation, small circulation volume, less liquid, good separation effect, compact structure design, small device size and small land occupation. BRIEF DESCRIPTION OF DRAWINGS

[0017] Fig. 1 It is a working structure schematic view of the electric ultrafiltration device for separating proteins in the preferred embodiment of the present application.

[0018] Fig. 2 It is a structure schematic view of the electric ultrafiltration device for separating proteins in the preferred embodiment of the present application.

[0019] The drawings show that: 1, electrolytic cell; 2, partition pad; 3, anode chamber; 4, cathode chamber; 5, raw liquid chamber; 6, dialysis chamber; 7, anode plate; 8, cathode plate; 9, ultrafiltration membrane mechanism; 901, ultrafiltration membrane support plate; 902, ultrafiltration membrane; 10, first anode membrane; 11, second anode membrane. DETAILED DESCRIPTION

[0020] The technical scheme of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments.

[0021] Reference Figs. 1-2 As shown in the preferred embodiment of this utility model, an electro-ultrafiltration device for separating proteins includes an electrolytic cell 1. The electrolytic cell 1 is provided with a dividing mechanism for dividing the electrolytic cell 1 into several functional chambers, so that each functional chamber of the device can be independent of each other, facilitating the separation of different protein products. The functional chambers include an anode chamber 3, a cathode chamber 4, a stock solution chamber 5, and a dialysis chamber 6. The stock solution chamber 5 and the dialysis chamber 6 are located between the anode chamber 3 and the cathode chamber 4. An anode plate 7 is installed in the anode chamber 3, a cathode plate 8 is installed in the cathode chamber 4, and an ultrafiltration membrane mechanism 9 is installed in the dialysis chamber 6. A first cation membrane 10 is installed in the stock solution chamber 5, and a second cation membrane 11 is installed in the dialysis chamber 6, thereby facilitating the cleaning and replacement of membranes. At the same time, the membranes can be recycled for a long time. The operation is simple and the operation is stable.

[0022] As a preferred embodiment of this utility model, it may also have the following additional technical features: The electrolytic cell 1 is provided with a plurality of partition gaskets 2, the partition gaskets 2 abutting against the inner wall of the electrolytic cell 1, and there are four partition gaskets 2, which are respectively located behind the anode plate 7, the first cation membrane 10, the second cation membrane 11, and the ultrafiltration membrane mechanism 9, so that each ion membrane in the functional chamber can be independent of each other, facilitating the separation of different protein products.

[0023] In this embodiment, the first cation membrane 10 and the second cation membrane 11 are symmetrically arranged in the electrolytic cell, with the first cation membrane 10 positioned closer to the anode plate 7 and the second cation membrane 11 positioned closer to the cathode plate 8. This allows sodium ions to pass through the cation membrane from the positive electrode, through the ultrafiltration membrane, and into the negative electrode chamber, thus generating a normal current effect inside the assembly.

[0024] In this embodiment, the ultrafiltration membrane mechanism 9 includes an ultrafiltration membrane support plate 901 installed in the dialysis chamber 6, and an ultrafiltration membrane 902 is installed on the ultrafiltration membrane support plate 901; the ultrafiltration membrane 902 is installed between the first cation membrane 10 and the second cation membrane 11. This allows ions to move in a directional manner under electrical drive, selectively passing through the ultrafiltration membrane for separation.

[0025] The working principle of the utility model is: in the electric ultrafiltration membrane device, the anode plate 7 side is equipped with the positive membrane 10, the cathode plate 8 side is equipped with the positive membrane 10, and the positive membrane 10 is equipped on both sides of the positive and negative electrode, so that sodium ions can pass through the positive membrane from the positive electrode, pass through the ultrafiltration membrane 902 and enter the cathode chamber 4. In this way, the normal current effect can be generated inside the assembly. In the raw solution chamber 5, the positive and negative protein ions migrate to the negative electrode under the action of the electric field, and the small-molecular-weight positively charged protein can pass through the ultrafiltration membrane 902. The large-molecular-weight negatively charged protein migrates to the anode. Due to the molecular weight and the electric field, the effect of separating the large-molecular-weight negatively charged protein and the small-molecular-weight positively charged protein is realized, and the concentration polarization on both sides of the membrane is greatly reduced, and the membrane pollution phenomenon is reduced.

[0026] Due to the action of the electric field, the positively charged small-molecular-weight protein migrates directionally and passes through the ultrafiltration membrane 902, and the negatively charged large-molecular-weight protein migrates to the anode, so that the purity of the target protein is greatly improved. Due to the small pore size of the cation and anion membrane, the protein molecules can be prevented from passing through the ion membrane and reacting in the electrode chamber.

[0027] If the product contains inorganic salts such as sodium chloride, the salt can be migrated to the electrode chamber in the system at the same time, and the product desalination effect is achieved. If the large-molecular-weight protein product needs to be concentrated, the pressure of the raw material chamber pump can be increased.

[0028] Without conflict, those skilled in the art can freely combine and superimpose the above additional technical features.

[0029] It can be understood that the utility model is described through some embodiments, and those skilled in the art know that various changes or equivalent replacements can be made to these features and embodiments without departing from the spirit and scope of the utility model. In addition, under the guidance of the utility model, these features and embodiments can be modified to adapt to specific conditions and materials without departing from the spirit and scope of the utility model. Therefore, the utility model is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of the present application belong to the scope protected by the utility model.

Claims

1. An electro-ultrafiltration apparatus for separating proteins, characterized by: The electrolytic tank is internally provided with a plurality of functional chambers, including an anode chamber, a cathode chamber, a raw solution chamber and a dialysis chamber, the anode chamber is internally provided with an anode plate, the cathode chamber is internally provided with a cathode plate, the dialysis chamber is internally provided with an ultrafiltration membrane mechanism, the raw solution chamber is provided with a first anode membrane, and the dialysis chamber is internally provided with a second anode membrane.

2. An electro-ultrafiltration apparatus for separating proteins according to claim 1, characterized in that: The electrolytic tank is internally provided with a plurality of partition gaskets, which abut against the inner wall of the electrolytic tank.

3. An electro-ultrafiltration apparatus for separating proteins according to claim 2, characterized in that: The four partition gaskets are respectively located behind the anode plate, the first anode membrane, the second anode membrane and the ultrafiltration membrane mechanism.

4. The electro-ultrafiltration apparatus for separating proteins of claim 1, wherein: The first anode membrane and the second anode membrane are symmetrically arranged in the electrolytic tank.

5. The electro-ultrafiltration device for separating proteins according to claim 1, characterized in that: The raw solution chamber and the dialysis chamber are arranged between the anode chamber and the cathode chamber.

6. The electro-ultrafiltration device for separating proteins according to claim 1, characterized in that: The first anode membrane is arranged close to one side of the anode plate, and the second anode membrane is arranged close to one side of the cathode plate.

7. The electro-ultrafiltration device for separating proteins according to claim 1, characterized in that: The ultrafiltration membrane mechanism comprises an ultrafiltration membrane support plate installed in the dialysis chamber, and the ultrafiltration membrane support plate is provided with an ultrafiltration membrane.

8. An electro-ultrafiltration apparatus for separating proteins according to claim 7, characterized in that: The ultrafiltration membrane is installed between the first anode membrane and the second anode membrane.

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