Subpackaging device capable of conveniently subpackaging microcarriers into hollow fiber columns

Abstract

The utility model belongs to the technical field of sub-packaging, and particularly discloses a sub-packaging device convenient for sub-packaging a microcarrier into a hollow fiber column, which comprises a shaking unit, an oscillating unit, an inclining unit and a first conveying unit, the shaking unit is used for shaking the container, and the oscillating unit is used for oscillating the hollow fiber column; the inclination unit is used for adjusting the inclination state of the hollow fiber column; the first conveying unit is used for conveying the microcarriers in the container into the hollow fiber column. The shaking unit enables the microcarrier suspension to be in a uniform mixing state all the time, the problem of uneven shaking speed caused by manual treatment is solved, and the product quality problem caused by uneven cell damage degree and uneven distribution due to uneven force is avoided. The vibration unit and the inclination unit are additionally arranged, so that the hollow fiber column is in an inclined vibration state, the problem that microcarriers are not uniformly distributed in the hollow fiber column due to manual height adjustment and manual beating is solved, manpower is saved, the influence of manual work on the product quality is reduced, and the product quality is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of split charging, especially relates to a split charging device facilitating microcarrier split charging to hollow fiber column. BACKGROUND

[0002] Microcarrier refers to microbeads with a diameter of 60-250 mu m and suitable for adherent cell growth. They are usually composed of natural dextran or various synthetic polymers, can provide a large adhesion area for cells, and support cell propagation into a monolayer on the carrier surface. In actual production, after cells grow on the microcarriers, the microcarriers need to be collected and split charged into the outer cavity of the hollow fiber column. However, the following problems may occur during the split charging process:

[0003] 1. During the split charging process, the microcarriers settle too quickly, so manual shaking of the glass bottle is required every 1 min to ensure that the content of the microcarriers split charged into each hollow fiber column is the same. However, this manual shaking method is time-consuming and labor-intensive, and since the shaking force varies from person to person, it may lead to uneven distribution of the microcarriers; too much shaking force may also damage the cells on the microcarriers, ultimately leading to problems with product quality.

[0004] 2. During the split charging process, the cell-covered microcarrier suspension is injected into one end of the hollow fiber column, and waste liquid is discharged from the other end. The height of the liquid inlet end of the fiber column needs to be continuously adjusted manually to form an inclined state, so that the microcarrier suspension fills the column and slowly moves downward. In addition, the fiber column needs to be manually tapped every 2 min to ensure uniform distribution of the microcarriers. However, since the height of the manual adjustment is not easy to control, there is a deviation in the height adjustment each time, which cannot meet the requirement that the hollow fiber column is filled with liquid, resulting in air bubbles and affecting the distribution of the microcarriers in the fiber column; moreover, manual tapping is easily affected by the pipeline and operation space, and the force is not uniform, which also affects the distribution of the microcarriers in the hollow fiber column, ultimately affecting the product quality. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a split charging device facilitating microcarrier split charging to a hollow fiber column to solve the problem that manual split charging, shaking, and tapping in the prior art affect product quality and are time-consuming and labor-intensive.

[0006] To achieve the above-mentioned purpose, the technical scheme of the utility model is as follows: a split charging device facilitating microcarrier split charging to a hollow fiber column, which comprises a shaking unit, a vibration unit, an inclined unit, and a first conveying unit; the shaking unit is used for shaking a container containing microcarriers; the vibration unit is used for vibrating the hollow fiber column; the inclined unit is used for adjusting the inclined state of the hollow fiber column; and the first conveying unit is used for conveying the microcarriers in the container into the hollow fiber column.

[0007] Further, the liquid outlet of the hollow fiber column is connected with a second conveying unit, which is used to convey the liquid discharged from the liquid outlet into the waste liquid collecting tank.

[0008] Further, the first conveying unit comprises a conveying hose and a peristaltic pump, the conveying hose is clamped on the pump head of the peristaltic pump, and the peristaltic pump is used to convey the microcarriers.

[0009] Further, the shaking unit comprises a shaking table and a connecting plate, the connecting plate is installed on the shaking table, and the container is fixed on the connecting plate.

[0010] Further, the connecting plate is a rubber plate.

[0011] Further, the oscillation unit comprises an oscillator and a fixed base, the tilting unit is installed on the fixed base, and the fixed base is installed on the oscillator.

[0012] Further, the tilting unit comprises a tilting base, an adjusting assembly and a fixing assembly, the adjusting assembly is fixed on the fixed base, one side of the tilting base is hinged to the fixed base, the other side of the tilting base is provided with a strip-shaped hole, a movable block is movably connected in the strip-shaped hole, and the adjusting assembly is hinged to the movable block; the adjusting assembly is used to adjust the tilting degree of the tilting base; and the fixing assembly is used to install the hollow fiber column on the tilting base.

[0013] Further, the fixing assembly comprises two groups of clamps, the two groups of clamps are respectively fixed on the two sides of the tilting base, and the two groups of clamps are used to clamp and fix the two sides of the hollow fiber column.

[0014] The working principle of the technical scheme is that the first conveying unit is connected with the peristaltic pump, the container and the hollow fiber column, the second conveying unit is connected with the waste liquid collecting tank and the liquid outlet of the hollow fiber column, the container with cell suspension is fixed on the shaking table through the rubber plate, the hollow fiber column is fixed on the tilting base through the clamps, the switches of the shaking table and the oscillator are started, the adjusting assembly is started, the appropriate height is selected, then the peristaltic pump is started, the speed is first set to 80r / min, when the liquid comes out of the waste liquid collecting tank, the pump speed is adjusted to 50r / min. The cell suspension in the container is pumped into the outer pipeline of the hollow fiber column through the peristaltic pump, the microcarriers are intercepted by the membrane filaments, and the liquid is discharged from the liquid outlet through the membrane filaments and enters the waste liquid collecting tank. After a period of operation, the sub-packaging is completed, and the switches of the peristaltic pump, the oscillator and the shaking table are closed. The first conveying unit and the second conveying unit are detached from the hollow fiber column, the cover of the hollow fiber column is screwed tightly, is put into a packaging bag, and corresponding labels are attached. After the pipeline is detached, it is cleaned, and the whole process of sub-packaging is completed.

[0015] The beneficial effects of the technical solution are:

[0016] 1. The rocker is added below the container, the microcarrier suspension is always in a mixed state, the problem of uneven shaking speed caused by manual processing is solved, manpower is saved, and the product quality problems caused by uneven cell damage and uneven distribution are avoided.

[0017] 2. The shaker is added below the hollow fiber column, the inclined unit is fixed on the shaker, the hollow fiber column is fixed thereon, the hollow fiber column is always in an inclined and vibrating state, the uneven distribution of the microcarrier in the hollow fiber column caused by manual height adjustment and manual beating is solved, manpower is saved, the influence of manual work on product quality is reduced, and the product quality of the company is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The structure of the present application is a convenient microcarrier split charging device for hollow fiber column.

[0019] Figure 2 The structure of the present application is a convenient microcarrier split charging device for hollow fiber column. Figure 1 The structure of the present application is a convenient microcarrier split charging device for hollow fiber column. DETAILED DESCRIPTION

[0020] The technical scheme in the embodiments of the present application will be described in detail below:

[0021] The reference signs in the drawings of the specification include: rocker 1, connecting plate 2, container 3, conveying hose 4, peristaltic pump 5, shaker 6, fixed base 7, adjusting assembly 8, inclined base 9, hollow fiber column 10, clamp 11, second conveying unit 12, waste liquid collection tank 13, liquid outlet 14, liquid inlet 15.

[0022] The technical scheme in the embodiments of the present application will be described in detail below:

[0023] The embodiments are basically as shown in the accompanying drawings Figures 1-2As shown: a kind of split device for the convenience of microcarrier to be split into hollow fiber column, including shaking unit, oscillation unit, tilt unit, first conveying unit and second conveying unit 12.Shaking unit is used to shake the container 3 loaded with microcarrier, and shaking unit includes shaking table 1 and connecting plate 2, connecting plate 2 is installed on shaking table 1, container 3 is fixed on connecting plate 2, and connecting plate 2 is made of adhesive plate.Oscillation unit is used to oscillate hollow fiber column 10, and oscillation unit includes oscillator 6 and fixed base 7, tilt unit is installed on fixed base 7, and fixed base 7 is installed on oscillator 6.Tilt unit is used to adjust the tilt state of hollow fiber column 10;Tilt unit includes tilt base 9, adjusting assembly 8 and fixing assembly;Adjusting assembly 8 is fixed on fixed base 7, one side of tilt base 9 is hinged to fixed base 7, the other side of tilt base 9 is provided with strip-shaped hole, movable block is movably connected in strip-shaped hole, and adjusting assembly 8 is hinged to movable block.Adjusting assembly 8 is used to adjust the tilt degree of tilt base 9, and adjusting assembly 8 can adopt linear driver.Of course, adjusting assembly 8 can not be connected with tilt base 9, but is only used to push tilt base 9 upward, and the side of tilt base 9 away from adjusting assembly 8 is also hinged to fixed base 7.Fixing assembly is used to install hollow fiber column 10 on tilt base 9, and fixing assembly includes two sets of clamps 11, two sets of clamps 11 are fixed on the two sides of tilt base 9 respectively, and two sets of clamps 11 are used to clamp and fix the two sides of hollow fiber column 10.First conveying unit is used to convey microcarrier in container 3 into hollow fiber column 10;First conveying unit includes conveying hose 4 and peristaltic pump 5, and conveying hose 4 specifically adopts silica gel tube.Conveying hose 4 is clamped on the pump head of peristaltic pump 5, and peristaltic pump 5 is used to convey microcarrier;One side of conveying hose 4 is inserted into container 3, and the other side is connected with liquid inlet 15 of hollow fiber column 10.Liquid outlet 14 of hollow fiber column 10 is connected with second conveying unit 12, and second conveying unit 12 is used to transmit the liquid discharged from liquid outlet 14 into waste liquid collection tank 13.

[0024] The specific implementation process is as follows:

[0025] The first conveying unit is connected with the peristaltic pump 5, the container 3 and the hollow fiber column 10, and the second conveying unit 12 is connected with the waste liquid collecting tank 13 and the liquid outlet 14 of the hollow fiber column 10. The container 3 with the cell suspension is fixed on the shaking table 1 through the rubber plate, the hollow fiber column 10 is fixed on the inclined base 9 through the clamp 11, the switches of the shaking table 1 and the oscillator 6 are started, the adjusting assembly 8 is started, the appropriate height is selected, then the peristaltic pump 5 is started, the speed is set as 80r / min at first, and when the liquid comes out of the waste liquid collecting tank 13, the pump speed is adjusted as 50r / min. The cell suspension in the container 3 is pumped into the outer pipeline of the hollow fiber column 10 through the peristaltic pump 5, the microcarriers are intercepted by the membrane filaments, and the liquid is discharged from the liquid outlet 14 and enters the waste liquid collecting tank 13. After a period of operation, the sub-packaging is completed, and the switches of the peristaltic pump 5, the oscillator 6 and the shaking table 1 are turned off. The first conveying unit and the second conveying unit 12 are detached from the hollow fiber column 10, the cover of the hollow fiber column 10 is screwed, the hollow fiber column 10 is put into a packaging bag, the corresponding label is attached, the pipeline is cleaned after being detached, and the whole process of sub-packaging is completed.

[0026] It should be noted that the relational terms herein such as first and second and the like are used solely to distinguish one entity or action from another, without necessarily requiring or implying any such actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can also include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0027] The above is only an embodiment of the present application, and the common knowledge of specific structures and characteristics in the scheme is not described in detail, the ordinary skilled in the art knows all the ordinary technical knowledge in the technical field of the present application before the application date or the priority date, can know all the prior art in the field and has the ability to apply the conventional experimental means before the date, the ordinary skilled in the art can improve and implement the present scheme under the enlightenment of the present application combined with their own ability, some typical known structures or known methods should not be an obstacle for the ordinary skilled in the art to implement the present application. It should be pointed out that, for those skilled in the art, without departing from the structure of the present application, a number of modifications and improvements can be made, which should be regarded as the protection scope of the present application, which will not affect the effect and practicality of the present application. The protection scope of the present application should be subject to the content of its claims, and the specific implementation mode and the like in the specification can be used to explain the content of the claims.

Claims

1. A split device for facilitating the splitting of microcarriers into a hollow fiber column, characterized by: The device comprises a shaking unit, a vibrating unit, a tilting unit and a first conveying unit; the shaking unit is used for shaking a container (3) containing microcarriers; the vibrating unit is used for vibrating a hollow fiber column (10); the tilting unit is used for adjusting the tilting state of the hollow fiber column (10); and the first conveying unit is used for conveying the microcarriers in the container (3) into the hollow fiber column (10).

2. A device for conveniently sub-packaging microcarriers to a hollow fiber column according to claim 1, characterized in that: The hollow fiber column (10) is connected with a second conveying unit (12) for conveying the liquid discharged from the liquid outlet (14) into a waste liquid collecting tank (13).

3. A device for facilitating the dispensing of microcarriers into a hollow fiber column according to claim 1, wherein: The first conveying unit comprises a conveying hose (4) and a peristaltic pump (5), the conveying hose (4) is clamped on the pump head of the peristaltic pump (5), and the peristaltic pump (5) is used for conveying the microcarriers.

4. A device for conveniently sub-packaging microcarriers to a hollow fiber column according to claim 1, characterized in that: The shaking unit comprises a shaking table (1) and a connecting plate (2), the connecting plate (2) is installed on the shaking table (1), and the container (3) is fixed on the connecting plate (2).

5. A device for facilitating the dispensing of microcarriers into a hollow fibre column according to claim 4, characterised in that: The connecting plate (2) is a rubber plate.

6. A device for conveniently sub-packaging microcarriers to a hollow fiber column according to claim 1, characterized in that: The vibrating unit comprises a vibrator (6) and a fixed base (7), the tilting unit is installed on the fixed base (7), and the fixed base (7) is installed on the vibrator (6).

7. A device for conveniently sub-packaging microcarriers to a hollow fiber column according to claim 6, characterized in that: The tilting unit comprises a tilting base (9), an adjusting assembly (8) and a fixing assembly; the adjusting assembly (8) is fixed on the fixed base (7), one side of the tilting base (9) is hinged to the fixed base (7), the other side of the tilting base (9) is provided with a strip-shaped hole, a movable block is movably connected in the strip-shaped hole, and the adjusting assembly (8) is hinged to the movable block; the adjusting assembly (8) is used for adjusting the tilting degree of the tilting base (9); and the fixing assembly is used for mounting the hollow fiber column (10) on the tilting base (9).

8. A device for facilitating the dispensing of microcarriers to a hollow fiber column according to claim 7, wherein: The fixing assembly comprises two groups of clamps (11), the two groups of clamps (11) are respectively fixed on the two sides of the tilting base (9), and the two groups of clamps (11) are used for clamping and fixing the two sides of the hollow fiber column (10).

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