Filtering and separating device for cell extraction

Abstract

The utility model relates to the technical field of filtration and separation, and discloses a filtration and separation device for cell extraction, which comprises a filter cartridge, the bottom surface of the filter cartridge is provided with a conical bottom, the inner wall of the filter cartridge is fixedly connected with a fixed step, the top surface of the fixed step is fitted with a snap ring, the bottom surface of the snap ring is fixedly connected with a filter screen frame, and the filter screen frame is fixedly connected with the conical bottom. A cover is arranged on the top surface of the filter cartridge, a clamping groove is formed in the bottom surface of the cover, a pressing step is arranged on the inner wall of the clamping groove, the top surface of the cover is communicated with an air guide port, a fixing ring is clamped on the outer wall of the filter cartridge, a connecting rod is fixedly connected to the outer wall of the fixing ring, and the upper end of the air guide port is communicated with an air pipe. In the utility model, the filter screen clings to the outer wall of the filter screen frame, and the filter screen frame and the snap ring are arranged on the fixed step, are aligned with the edge of the top surface of the filter cartridge and are pressed, so that the filter screen frame is stable, liquid is prevented from sliding off from the edge of the snap ring, and the pressure pump accelerates filtration and ensures the filtration effect and rate.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of filtration separation, especially to a filtration separation device for cell extraction. BACKGROUND

[0002] Cell extraction refers to the process of separating specific cells from biological tissues, organs and cell cultures, which is crucial in the fields of biological research, medical diagnosis and treatment. In basic research, scientists obtain specific cells through cell extraction to explore the physiological functions, metabolic pathways and gene expression regulation mechanisms of cells, thereby gaining a deeper understanding of the basic laws of life activities. In the field of disease diagnosis, pathological analysis of cells extracted from diseased tissues in patients helps to confirm and classify diseases. In the emerging field of cell therapy, cell extraction is a critical initial step.

[0003] Cell extraction is a fundamental and critical operation. Accurate and efficient extraction of target cells from complex biological samples is essential for disease diagnosis, drug development, cell therapy and basic biological research. As an efficient and gentle separation method, filtration separation technology has great application potential in the field of cell extraction. Through a filtration separation device with appropriate pore size, material and structure, specific cells can be selectively retained or passed through according to their characteristics, achieving efficient separation and extraction. However, the current filtration separation device has the problem of poor filtration effect due to liquid sliding off the edge of the filter paper during filtration. In addition, the filtration speed is slow due to the small pore size of the filter used for cell extraction. SUMMARY

[0004] To overcome the above problems, the present utility model provides a filtration separation device for cell extraction, which aims to improve the filtration separation device in the prior art, which can prevent liquid from sliding off the edge of the filter paper during filtration, resulting in poor filtration effect. In addition, the filtration speed is slow due to the small pore size of the filter used for cell extraction.

[0005] To achieve the above purpose, the utility model adopts the following technical scheme: a filtration separation device for cell extraction, comprising a filter cartridge, the bottom surface of the filter cartridge is provided with a conical bottom, the inner wall of the filter cartridge is fixedly connected with a fixed step, the top surface of the fixed step is attached with a snap ring, the bottom surface of the snap ring is fixedly connected with a filter screen frame, the top surface of the filter cartridge is provided with a lid, the bottom surface of the lid is provided with a clamping groove, the inner wall of the clamping groove is provided with a pressing step, the top surface of the lid is communicated with a gas guide port, the lower end of the conical bottom is provided with a sealing mechanism, and the sealing mechanism is used for sealing collection.

[0006] Further description of the above technical scheme:

[0007] The sealing mechanism comprises a discharge port, the top of the discharge port is communicated with the bottom surface of the conical bottom, a threaded groove is formed in the outer wall of the bottom end of the discharge port, a collecting bottle is arranged at the lower end of the discharge port, a fixing ring is arranged at the top end of the collecting bottle, a rotating sleeve is slidably connected to the upper end of the collecting bottle, a limiting ring is arranged at the bottom end of the inner wall of the rotating sleeve, and the inner wall of the rotating sleeve is threadedly connected with the bottom surface of the discharge port.

[0008] As a further description of the above technical solution:

[0009] The outer wall of the filter cartridge is clamped with a fixing ring, the outer wall of the fixing ring is fixedly connected with a connecting rod, and the other end of the connecting rod is fixedly connected with a sleeve.

[0010] As a further description of the above technical solution:

[0011] The inner wall of the sleeve is slidably connected with a support, and the bottom surface of the support is fixedly connected with a base.

[0012] As a further description of the above technical solution:

[0013] The upper end of the air inlet is communicated with an air pipe, and the other end of the air pipe is communicated with a pressure pump.

[0014] As a further description of the above technical solution:

[0015] The outer wall of the sleeve is threadedly connected with a fastening screw, and the middle portion of the discharge port is provided with a valve.

[0016] As a further description of the above technical solution:

[0017] The bottom surface of the base is threadedly connected with a screw rod, and the outer wall of the screw rod is threadedly connected with a nut.

[0018] As a further description of the above technical solution:

[0019] The bottom end of the screw rod is fixedly connected with a ball, and the outer wall of the ball is rotatably connected with a foot.

[0020] The utility model has the advantages of the following:

[0021] 1、The filter screen is placed on the top of the filter screen frame before filtering, the filter paper is tightly attached to the outer wall of the filter screen frame, the filter screen frame and the snap ring are placed on the fixed step, the snap-in groove is aligned with the top surface edge of the filter cartridge, and the filter screen frame is tightly pressed, so that the filter screen frame is stably fixed, the filtered liquid cannot slide down from the edge of the snap ring, the pressure pump is started to provide pressure, the filtering speed is accelerated, the filtering effect is guaranteed, and the filtering rate is accelerated.

[0022] 2. In the utility model, when installing the collecting bottle, first, the top of the rotating sleeve is aligned with the bottom of the discharge port, the rotating sleeve is rotated, the rotating sleeve is tightly fixed at the bottom of the discharge port, the cooperation of the fixing ring and the limiting ring makes the rotating sleeve not separate from the collecting bottle, and the rotating sleeve can rotate freely, the top surface of the fixing ring is tightly attached to the bottom surface of the discharge port, and it is guaranteed that the filtered liquid is not polluted by air. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 A front side perspective view of the filtering and separating device for cell extraction is provided for the utility model;

[0024] Figure 2 A partial structure exploded view of the filter screen frame of the filtering and separating device for cell extraction is provided for the utility model;

[0025] Figure 3 A partial structure view of the cover of the filtering and separating device for cell extraction is provided for the utility model;

[0026] Figure 4 A partial structure exploded view of the rotating sleeve of the filtering and separating device for cell extraction is provided for the utility model;

[0027] Figure 5 A partial structure schematic view of the footing of the filtering and separating device for cell extraction is provided for the utility model.

[0028] LEGEND:

[0029] 1, filter cartridge;2, sealing mechanism;201, discharge port;202, threaded groove;203, rotating sleeve;204, limiting ring;205, collecting bottle;206, fixing ring;3, cone bottom;4, fixed step;5, clamping ring;6, filter screen frame;7, cover;8, clamping groove;9, pressing step;10, air guide port;11, fixing ring;12, connecting rod;13, sleeve;14, support;15, base;16, air pipe;17, pressure pump;18, screw;19, nut;20, ball;21, footing;22, valve;23, fastening screw. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0031] Please refer to the drawings in the embodiments of the utility model Figure 1 - the drawings in the embodiments of the utility model Figure 3The utility model provides a kind of cell extraction with filter separation device, including filter cartridge 1, the bottom surface of filter cartridge 1 is provided with cone bottom 3, the inner wall of filter cartridge 1 is fixedly connected with fixed step 4, the top surface of fixed step 4 is attached with snap ring 5, the bottom surface of snap ring 5 is fixedly connected with filter screen frame 6, the top surface of filter cartridge 1 is provided with lid 7, the bottom surface of lid 7 is provided with card slot 8, the inner wall of card slot 8 is provided with compression step 9, the top surface of lid 7 is communicated with gas guide 10, the lower end of cone bottom 3 is provided with sealing mechanism 2, sealing mechanism 2 is used for sealing collection;

[0032] Specifically, the bottom surface of the filter cartridge 1 is a cone bottom 3, which helps to concentrate the filtered liquid and facilitates collection. The inner wall of the filter cartridge 1 is fixedly connected with a fixed step 4, which not only serves as a structural support but also ensures the stability of the internal components of the filter cartridge 1. On the top surface of the fixed step 4, a snap ring 5 is attached, providing a platform for installing the filter screen frame 6. The filter screen frame 6 is a crucial part of the filtration process, supporting the filter medium and allowing liquid to pass while retaining cell solids. The top surface of the filter cartridge 1 is provided with a lid 7, which protects the internal components from external contamination. The bottom surface of the lid 7 is provided with a card slot 8, allowing the lid 7 to be fixed to the filter cartridge 1. The inner wall of the card slot 8 is provided with a compression step 9, providing additional compression force when the lid 7 is installed, ensuring airtightness. The top surface of the lid 7 is connected with a gas guide 10.

[0033] Please refer to the attached Figure 4 - attached Figure 5 The sealing mechanism 2 includes a discharge port 201, the top of the discharge port 201 is connected with the bottom surface of the cone bottom 3, the bottom end outer wall of the discharge port 201 is provided with a threaded groove 202, the lower end of the discharge port 201 is provided with a collection bottle 205, the top end of the collection bottle 205 is provided with a fixing ring 206, the upper end of the collection bottle 205 is slidingly connected with a rotating sleeve 203, the inner wall bottom end of the rotating sleeve 203 is provided with a limiting ring 204, the inner wall of the rotating sleeve 203 is threadedly connected with the bottom surface of the discharge port 201.

[0034] Specifically, the upper end of the discharge port 201 is directly connected with the lower surface of the cone bottom 3, and the lower end of the discharge port 201 is designed with a threaded groove 202 on the outer side, so that the discharge port 201 can be effectively connected and fixed. A collection bottle 205 is arranged below the discharge port 201, which is used to collect the material flowing out of the discharge port 201. In order to ensure the stability of the collection bottle 205 and facilitate replacement, the top end is designed with a fixing ring 206. The upper end of the collection bottle 205 is slidingly connected with a rotating sleeve 203, the inner wall bottom of the rotating sleeve 203 is provided with a limiting ring 204 for limiting displacement, and the inner wall of the rotating sleeve 203 is threadedly connected with the bottom surface of the discharge port 201, which ensures the sealing performance and facilitates the disassembly and installation of the rotating sleeve 203.

[0035] Please refer to the attached Figure 1 - attached Figure 3 , the outer wall of the filter cartridge 1 is engaged with the fixed ring 11, the outer wall of the fixed ring 11 is fixedly connected with the connecting rod 12, the other end of the connecting rod 12 is fixedly connected with the sleeve 13, the inner wall of the sleeve 13 is slidingly connected with the support 14, the bottom surface of the support 14 is fixedly connected with the base 15, the upper end of the air guide port 10 is communicated with the air pipe 16, the other end of the air pipe 16 is communicated with the pressure pump 17;

[0036] Specifically, the outer wall of the filter cartridge 1 is tightly fitted with the fixed ring 11, the fixed ring 11 is arranged on the outer side of the filter cartridge 1, a sturdy connecting rod 12 is fixedly connected to the outer wall of the fixed ring 11, the other end of the connecting rod 12 is firmly connected with the sleeve 13, ensuring the stability of the entire structure, the inner wall of the sleeve 13 can smoothly slide on the outer wall of the support 14, thereby realizing flexible adjustment function, the bottom surface of the support 14 is fixedly connected with the base 15, providing a stable support foundation for the entire device, the upper end of the air guide port 10 is connected with the air pipe 16, the other end of the air pipe 16 is communicated with the pressure pump 17, thereby ensuring the continuity of gas flow and stable supply of pressure.

[0037] Please refer to the attached Figure 3 - attached Figure 5 , the outer wall of the sleeve 13 is threadedly connected with the fastening screw 23, the middle part of the discharge port 201 is provided with a valve 22, the bottom surface of the base 15 is threadedly connected with a screw rod 18, the outer wall of the screw rod 18 is threadedly connected with a nut 19, the bottom end of the screw rod 18 is fixedly connected with a ball 20, the outer wall of the ball 20 is rotatably connected with a foot 21;

[0038] Specifically, the outer wall of the sleeve 13 is connected with the fastening screw 23 by thread, to ensure its stability and reliability, the middle part of the discharge port 201 is specially designed with a valve 22 to facilitate control of the outflow of materials, the bottom surface of the base 15 is fixed with a screw rod 18 by thread connection, further enhancing the stability of the structure, the outer wall of the screw rod 18 is also connected with the nut 19 by thread connection, making the adjustment of the entire device more flexible and convenient, in order to realize more accurate positioning and support, the bottom end of the screw rod 18 is fixedly connected with a ball 20, the outer wall of the ball 20 is rotatably connected with a foot 21, which can ensure that the device can maintain good stability and adaptability in different working environments.

[0039] Working principle: before filtering, place the filter screen on the top of the filter screen frame 6, make the filter paper tightly adhere to the outer wall of the filter screen frame 6, place the filter screen frame 6 and the snap ring 5 on the fixed step 4, align the clamping groove 8 to the top edge of the filter cylinder 1, press tightly, at this time, the pressing step 9 presses the top of the snap ring 5, so that the filter screen frame 6 is stably fixed, when the filtered liquid will not slide from the edge of the snap ring 5, start the pressure pump 17 to provide pressure, accelerate the filtering speed, ensure the filtering effect, and accelerate the filtering rate;

[0040] When the collecting bottle 205 is installed, first, the top of the rotating sleeve 203 is aligned with the bottom of the discharge port 201, the rotating sleeve 203 is rotated, the rotating sleeve 203 is tightly fixed at the bottom of the discharge port 201, the cooperation of the fixing ring 206 and the limiting ring 204 makes the rotating sleeve 203 not to be separated from the collecting bottle 205, and the rotating sleeve 203 can be freely rotated, the top surface of the fixing ring 206 is tightly adhered to the bottom surface of the discharge port 201, so that the filtered liquid is not polluted by air.

[0041] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can be modified to the technical solutions recorded in the foregoing embodiments, or equivalent replacement for part of the technical features, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application, should be included in the protection scope of the present application.

Claims

1. A filtration separation device for cell extraction comprising a filter cartridge (1), characterized in that: The bottom surface of the filter cartridge (1) is provided with a conical bottom (3), the inner wall of the filter cartridge (1) is fixedly connected with a fixed step (4), the top surface of the fixed step (4) is attached with a snap ring (5), the bottom surface of the snap ring (5) is fixedly connected with a filter screen frame (6), the top surface of the filter cartridge (1) is provided with a cover (7), the bottom surface of the cover (7) is provided with a clamping groove (8), the inner wall of the clamping groove (8) is provided with a pressing step (9), the top surface of the cover (7) is communicated with a gas guide port (10), the lower end of the conical bottom (3) is provided with a sealing mechanism (2), and the sealing mechanism (2) is used for sealing collection.

2. The filtration separation device for cell extraction according to claim 1, characterized in that: The sealing mechanism (2) comprises a discharge port (201), the top of the discharge port (201) is communicated with the bottom surface of the conical bottom (3), the bottom end outer wall of the discharge port (201) is provided with a threaded groove (202), the lower end of the discharge port (201) is provided with a collection bottle (205), the top end of the collection bottle (205) is provided with a fixed ring (206), the upper end of the collection bottle (205) is slidably connected with a rotating sleeve (203), the inner wall bottom end of the rotating sleeve (203) is provided with a limiting ring (204), and the inner wall of the rotating sleeve (203) is threadedly connected with the bottom surface of the discharge port (201).

3. The filtration separation device for cell extraction according to claim 2, characterized in that: The outer wall of the filter cartridge (1) is clamped with a fixed ring (11), the outer wall of the fixed ring (11) is fixedly connected with a connecting rod (12), and the other end of the connecting rod (12) is fixedly connected with a sleeve (13).

4. The filtration separation device for cell extraction according to claim 3, characterized in that: The inner wall of the sleeve (13) is slidably connected with a support (14), and the bottom surface of the support (14) is fixedly connected with a base (15).

5. The filtration separation device for cell extraction according to claim 4, characterized in that: The upper end of the gas guide port (10) is communicated with a gas pipe (16), and the other end of the gas pipe (16) is communicated with a pressure pump (17).

6. The filtration separation device for cell extraction according to claim 5, wherein: The outer wall of the sleeve (13) is threadedly connected with a fastening screw (23), and the middle part of the discharge port (201) is provided with a valve (22).

7. The filtration separation device for cell extraction according to claim 6, characterized in that: The bottom surface of the base (15) is threadedly connected with a screw rod (18), and the outer wall of the screw rod (18) is threadedly connected with a nut (19).

8. The filtration separation device for cell extraction according to claim 7, characterized in that: The bottom end of the screw rod (18) is fixedly connected with a ball (20), and the outer wall of the ball (20) is rotatably connected with a foot (21).

Images