Quartered confocal vessel for cell observation
By designing a four-compartment confocal dish, the problems of high cost and poor compatibility of existing confocal dishes are solved, and the effects of simultaneous culture of multiple samples, clear observation and low contamination are achieved, making it suitable for cell observation experiments.
Patent Information
- Application Number
- CN202423320755.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2034-12-30
AI Technical Summary
Existing confocal consumables are expensive, have limited compatibility, cannot process multiple samples simultaneously, and cannot meet the needs of different scenarios and have strict sample processing requirements.
Design a four-compartment confocal dish. The interior of the dish is divided into four equal-area compartments. It is equipped with a high-transmittance optical glass bottom and a sealing structure. The material is black PS material holder color masterbatch. The outside is provided with anti-slip texture and sealing gasket. The dish lid and dish body are sealed by a slot.
It enables simultaneous culture of multiple cell types, avoids cross-contamination, provides a clear field of view, reduces the risk of external contamination, ensures the accuracy and reliability of experimental results, is suitable for long-term observation, and improves compatibility and safety.
Smart Images
Figure CN223852612U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to a kind of medical instrument field, more specifically, it relates to a four-part grid confocal dish for cell observation. BACKGROUND
[0002] In recent years, with the rapid development of life sciences and materials science and other fields, the demand for confocal dish consumables is increasing, and its types and specifications are also increasingly rich. From the initial simple glass bottom culture dish, to the later confocal culture dish without coating, to the multi-well plate and high-content microscope dedicated confocal dish consumables, these advances not only improve the efficiency and accuracy of experiments, but also reduce the complexity and cost of experimental operations.
[0003] Although confocal dish consumables have broad application prospects and importance in confocal microscope experiments, their high cost, compatibility limitations, inability to handle multiple samples simultaneously, inability to meet different scene requirements, and strict sample processing requirements also need to be fully considered and addressed in practical applications. SUMMARY
[0004] In view of the deficiencies of the prior art, the utility model aims to provide a four-part grid confocal dish for cell observation with strong sealing and compatibility.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a four-part grid confocal dish for cell observation, comprising a dish body and a dish cover matched with the dish body, the dish body is a circular structure, the inside of the dish body is divided into four equal-area compartments by a separation structure, each compartment is independent of each other, and the bottom of the dish body is provided with high-transmittance optical glass.
[0006] The utility model is further provided as follows: the diameter of the optical glass is between 1 / 2 and 2 / 3 of the diameter of the dish body.
[0007] The utility model is further provided as follows: the dish body is also provided with a plurality of evenly distributed clamping grooves, and the bottom of the dish cover is provided with clamping sites matched with each clamping groove.
[0008] The utility model is further provided as follows: the height of the separation structure is the same as the depth of the dish body, and the top edge of the separation structure is arc-shaped.
[0009] The utility model is further provided as follows: the dish body is made of black PS material frame color master batch.
[0010] The utility model is further provided as follows: the outer sidewall of the dish body is provided with anti-slip texture, and the connection between the dish body and the dish cover is provided with a sealing gasket.
[0011] The utility model has the advantages of:
[0012] 1. Four equal-area compartments are independent of each other, and can carry out multiple cell cultures under the same culture conditions at the same time, compare the effects of different drugs on cells or test the reactions of cells to different environments, without the need for multiple culture dishes, saving space and cost, avoiding cross contamination between cells, ensuring the purity of each sample, especially in comparative experiments, ensuring that the results are only related to the variables studied, and the data is more reliable, the high-transmittance optical glass set at the bottom provides a clear field of view for confocal microscope observation, reduces light scattering and refraction, and accurately captures cell details; the circular structure matches the dish cover, which is convenient to take and move, can reduce the risk of external pollution, and is beneficial to long-term cell observation experiments.
[0013] 2. When the diameter of the optical glass is less than 1 / 2 of the diameter of the dish body, the observation field of view is limited, affecting the observation effect; when the diameter of the optical glass is greater than 2 / 3 of the diameter of the dish body, the size is too large, causing installation inconvenience or increasing the overall weight and thickness of the dish body, therefore, the diameter of the optical glass is between 1 / 2 and 2 / 3 of the diameter of the dish body, which is the best, can occupy a suitable proportion at the bottom of the dish body, and ensure a large and clear observation area, which is convenient for comprehensive and detailed observation of cells in each compartment through confocal microscope and other equipment, the clamping groove and the clamping position cooperate to tightly cover the dish cover on the dish body, effectively blocking dust, microorganisms and other pollutants from entering, creating a relatively clean and stable internal environment for cell culture, which is beneficial to the normal growth and observation of cells, and moreover, the design of the dish cover can adapt to conventional sizes, improving compatibility.
[0014] 3. The dish body is made of black PS material, which is beneficial to fluorescence staining or dynamic observation of super-high-resolution imaging, the height of the separation structure is the same as the depth of the dish body, which can better separate each compartment, and when adding culture solution, cell samples and other operations, there is no need to worry about the liquid flowing freely between the compartments and affecting the independent experimental environment in each compartment, which is more convenient to operate, the arc-shaped top edge can avoid the liquid residue and wall hanging phenomenon caused by the straight angle edge, and can also reduce the splashing of the liquid during the adding process due to collision with sharp corners, ensuring the accuracy of the liquid amount in each compartment and the cleanliness of the experimental environment.
[0015] 4. The outer side wall of the dish body is provided with anti-slip texture, which can increase the friction between the hands and the dish body when taking, moving or operating the confocal dish, effectively preventing accidents such as dish falling and overturning caused by slippery hands, and ensuring the safety of cell samples and the smooth progress of experiments, a sealing gasket is arranged at the connection between the dish body and the dish cover, which can further fill the small gap between them, preventing the leakage of culture solution from polluting the environment, and preventing foreign matter from entering the dish, affecting the accuracy of experimental results. BRIEF DESCRIPTION OF DRAWINGS
[0016] Fig. 1It is a structure schematic view of the dish body;
[0017] Fig. 2 It is a structure schematic view of the dish cover;
[0018] Fig. 3 It is a sectional view of the dish body and the dish cover after installation;
[0019] Figs. 1-3 Reference signs: 1, dish body; 2, dish cover; 3, separation structure; 4, optical glass; 5, clamping groove; 6, clamping position; 7, compartment. DETAILED DESCRIPTION
[0020] Reference Figs. 1 to 3 The embodiment of the utility model is further explained.
[0021] For ease of explanation, spatial relative terms such as "upper", "lower", "left", "right" and the like are used in the embodiments to describe the relationship of one element or feature shown in the drawing relative to another element or feature. It should be understood that, in addition to the orientation shown in the drawing, the spatial terms are intended to include different orientations of the device in use or operation. For example, if the device in the drawing is inverted, the element described as being located "below" other elements or features will be positioned "above" the other elements or features. Therefore, the exemplary term "below" can include both the upper and lower orientations. The device can be positioned in other ways (rotated 90 degrees or positioned in other orientations), and the spatial relative description used herein can be interpreted accordingly.
[0022] Moreover, relational terms such as "first" and "second" and the like are merely used to distinguish one from another of similar names, and do not necessarily require or imply any such actual relationship or order between the components.
[0023] Figs. 1 to 3 The four-quadrant confocal dish for cell observation shown comprises a dish body 1 and a dish cover 2 matched with the dish body 1, the dish body 1 is a circular structure, the circular structure is matched with the dish cover 2, which is convenient to take and move and can reduce the risk of external pollution, is beneficial to long-time cell observation experiment, the inside of the dish body 1 is divided into four equal-area compartments 7 through a separation structure 3, the compartments 7 are independent of each other, the four equal-area compartments 7 are independent of each other, can carry out multiple cell culture, compare the effects of different drugs on cells or test the reactions of cells to different environments under the same culture condition, do not need multiple culture dishes, save space and cost, avoid cross contamination between cells, ensure the purity of each sample, especially in the comparative experiment, ensure that the result is only related to the studied variable, the data is more reliable, the bottom of the dish body 1 is provided with optical glass 4 with high light transmittance, which provides a clear field of view for confocal microscope observation, reduces light scattering and refraction, and accurately captures cell details.
[0024] When the diameter of the optical glass 4 is less than 1 / 2 of the diameter of the dish body 1, the observation field of view is limited, affecting the observation effect; when the diameter of the optical glass 4 is greater than 2 / 3 of the diameter of the dish body 1, the size is too large, causing inconvenience in installation or increasing the overall weight and thickness of the dish body 1, therefore, the diameter of the optical glass 4 is preferably between 1 / 2 and 2 / 3 of the diameter of the dish body 1, which can occupy a suitable proportion at the bottom of the dish body 1, ensuring a large and clear observation area, and facilitating comprehensive and detailed observation of cells in each cell chamber 7 through a confocal microscope or the like.
[0025] The dish body 1 is also provided with a plurality of clamping grooves 5 uniformly distributed thereon, and the bottom of the dish cover 2 is provided with clamping positions 6 matched with the clamping grooves 5; the clamping grooves 5 and the clamping positions 6 cooperate to allow the dish cover 2 to be tightly covered on the dish body 1, effectively preventing dust, microorganisms and other pollutants from entering, creating a relatively clean and stable internal environment for cell culture, which is conducive to the normal growth and observation of cells, and moreover, the design of the dish cover 2 can adapt to conventional sizes, improving compatibility.
[0026] The dish body 1 is made of black PS material, which is beneficial to fluorescence staining or dynamic observation of super-high resolution imaging, and the height of the separation structure is the same as the depth of the dish body 1, which can better separate the cell chambers 7, so that the liquid does not flow between the cell chambers 7 during the operation of adding culture solution and cell samples, thereby affecting the independent experimental environment in each cell chamber 7, and the operation is more convenient, the arc-shaped top edge can avoid the liquid residue and wall-hanging phenomenon caused by the straight edge, and can also reduce the splashing of the liquid during the adding process due to collision with sharp corners, thereby ensuring the accuracy of the liquid amount in each cell chamber 7 and the cleanliness of the experimental environment.
[0027] The arc-shaped edge is not easy to hide dirt, which is convenient for subsequent maintenance work such as cleaning and disinfecting of the confocal dish, further reducing the possibility of cell sample pollution caused by bacteria breeding due to residual impurities, and is conducive to repeated use and accurate development of cell observation experiments.
[0028] The outer side wall of the dish body 1 is provided with anti-skid texture, which can increase the friction between the hands and the dish body 1 when taking, moving or operating the confocal dish, effectively preventing accidents such as falling and overturning of the dish body 1 due to slippery hands, and ensuring the safety of the cell samples and the smooth progress of the experiment, and a sealing gasket is arranged at the connection between the dish body 1 and the dish cover 2, which can further fill the small gap between them, preventing the leakage of the culture solution from polluting the environment, and preventing foreign matter from entering the dish, affecting the accuracy of the experimental results.
[0029] The above only describes the preferred embodiments of the present application, and is not intended to limit the present application, and any usual changes and replacements within the technical scheme of the present application should be included in the protection scope of the present application.
Claims
1. A quadripartite confocal dish for cell observation, comprising a dish body (1) and a dish cover (2) matched with the dish body (1), characterized in that, The dish body (1) is circular structure, the dish body (1) inside is divided into four equal area compartments (7) by separating structure (3), each the compartment (7) is independent, the bottom of the dish body (1) is equipped with high light transmission optical glass (4).
2. The quadripartite confocal dish for cell observation according to claim 1, characterized in that, The diameter of the optical glass (4) is between 1 / 2 and 2 / 3 of the diameter of the dish body (1).
3. The quadripartite confocal dish for cell observation according to claim 1, characterized in that, The dish body (1) is also provided with a plurality of clamping grooves (5) uniformly distributed, the bottom of the dish cover (2) is provided with a clamping position (6) matched with each clamping groove (5).
4. The quadripartite confocal dish for cell observation according to claim 1, characterized in that, The height of the separating structure is the same as the depth of the dish body (1), and the top edge of the separating structure is arc-shaped.
5. The quadripartite confocal dish for cell observation according to claim 1, characterized in that, The material of the dish body (1) is black PS material frame color master batch.
6. The quadripartite confocal dish for cell observation according to claim 1, characterized in that, The outer side wall of the dish body (1) is provided with anti-skid texture, and the connecting part of the dish body (1) and the dish cover (2) is provided with a sealing gasket.