Automatic food protease activity measuring device

Abstract

An automatic food protease activity determination device belongs to the technical field of food detection and comprises a base, a PLC comprehensive control panel and a light transmittance analyzer body are fixedly mounted on the base, two placement grooves are formed in the right side of the upper surface of the base, detection cups are placed in the placement grooves, and a detection pool is formed in the left side of the upper surface of the base. Two cup body limiting columns are fixedly mounted in the detection pool in a bilateral symmetry manner, a light-transmitting cup is clamped between the two cup body limiting columns, a light source body and a light-transmitting detection piece are fixedly mounted on the front side wall and the rear side wall in the detection pool respectively, and the light-transmitting detection piece is electrically connected with the light transmittance analyzer main body; a shading cover plate used for sealing and covering the detection pool is rotatably mounted on the left side of the upper surface of the base, and a lifting rotary liquid transfer frame is mounted in the center of the upper surface of the base. When liquid is taken, the displacement of a piston of an injector can be accurate to a micro scale, the liquid taking accuracy is remarkably improved, errors are reduced, and the accuracy of food protease activity determination is ensured.

Description

TECHNICAL FIELD

[0001] The utility model belongs to food detection technical field, concretely relates to a kind of automatic food protease activity determination device. BACKGROUND

[0002] In food industry, protease is a kind of extremely important biological catalyst, and its accurate determination has key significance to food quality control, processing technology optimization and food safety guarantee etc..For example, in dairy processing, protease activity affects the maturity and quality of cheese;In meat processing, it is closely related to the degree of meat tenderization.

[0003] Traditional food protease activity determination method is mostly dependent on manual operation. First, food sample is collected manually and pretreated to extract protease therein. Subsequently, in determination process, operator needs to manually transfer protease solution to specific reaction container, such as test tube or cuvette, and use pipette or other tools to accurately measure substrate solution, buffer solution and other reagents drop by drop into reaction container. After reaction is completed, reaction mixture is transferred to detection cell of colorimeter or spectrophotometer for light transmission detection to determine the decomposition degree of substrate by protease, and then calculate protease activity.

[0004] Manual operation is difficult to ensure the accuracy and repeatability of each time pipetting, even experienced operator, it is also difficult to avoid tiny error, and these errors may accumulate in multiple repeated determination process, resulting in large deviation of final determination result, which cannot meet the requirement of high-precision determination. SUMMARY

[0005] In view of the problem in the prior art that when determining food protein enzyme activity, manual operation of a pipette for pipetting has low accuracy and is prone to errors, affecting the determination result, the utility model provides an automatic food protein enzyme activity determination device, precise control of a syringe is realized by using a lifting rotary pipetting rack, under the assistance of the vertical lifting function, two syringes are driven by the lifting device to move upward on the piston rod, protein enzyme liquid and bottom liquid are respectively extracted from two detection cups, then the lifting rotary pipetting rack moves the two syringes out of the detection cups and rotates them to above the light transmission cup in turn, liquid is injected into the light transmission cup, then the light shielding cover plate is closed to create a dark detection pool environment, the light source body is turned on, after the light penetrates the light transmission cup and the protein enzyme and bottom liquid mixture therein, it reaches the light transmission detection piece, because the mixture absorbs part of the light energy, the light intensity attenuates, the light absorption spectrum is obtained by measuring the light absorption degree of the mixture, and then the enzyme activity is calculated according to the comparison between the absorbance at a specific wavelength and the light absorption degree of the bottom liquid. The device mechanically completes the extraction and transfer of protein enzyme liquid and droplets to the light transmission cup for determination, the displacement of the syringe piston can be accurately controlled to a small scale when liquid is taken, the liquid taking precision is significantly improved, errors are reduced, the problem that the accuracy of manual pipetting is poor and errors are prone to occur in the determination of food protein enzyme activity is effectively solved, and a high-reliability and high-precision automatic solution is provided for food protein enzyme activity determination.

[0006] The specific technical solutions are as follows:

[0007] An automatic food protein enzyme activity determination device, comprising a base, a PLC comprehensive control panel and a transmittance analyzer main body are fixedly installed on the base, two placing grooves are formed in the right side of the upper surface of the base, detection cups are placed in the placing grooves, a detection pool is formed in the left side of the upper surface of the base, two cup body limiting columns are fixedly installed in the detection pool and are symmetrical about the left-right direction, a light transmission cup is clamped and placed between the two cup body limiting columns, a light source body and a light transmission detection piece are fixedly installed on the front and rear side walls of the detection pool respectively, the light transmission detection piece is electrically connected with the transmittance analyzer main body, a light shielding cover plate for covering the detection pool is rotatably installed on the left side of the upper surface of the base, a lifting rotary pipetting rack is installed at the center position of the upper surface of the base, two syringes are fixedly installed at the right end of the lifting rotary pipetting rack, two lifting devices are installed on the right side of the upper surface of the lifting rotary pipetting rack, clamping devices are installed in the lifting devices, the top ends of the syringes are clamped and installed in the corresponding clamping devices, and the syringes are located above the corresponding detection cups.

[0008] In the technical scheme, the lifting and rotating liquid transferring frame comprises a movable carrier, an electric telescopic rod, a carrier plate and an angle adjusting motor, the angle adjusting motor is fixedly installed on the upper surface of the base, the output end of the angle adjusting motor is fixedly connected with the center of the lower surface of the movable carrier, the electric telescopic rod is fixedly installed on the upper surface of the movable carrier, the upper surface of the electric telescopic rod is fixedly connected with the lower surface of the carrier plate, two extension plates are integrally arranged on the right side of the carrier plate, a through hole is formed in the right end of each extension plate, and a syringe is inserted and connected in the corresponding through hole, and a clamping bolt for fixing the syringe is screw-connected and installed on the right surface of the extension plate.

[0009] In the technical scheme, the upper surface of the base is symmetrically fixedly installed with two arc-shaped limiting blocks, and the movable carrier is rotatably installed between the two arc-shaped limiting blocks.

[0010] In the technical scheme, the lifting device comprises a micro stepping motor, a U-shaped frame and a screw rod, the U-shaped frame and the micro stepping motor are fixedly installed on the upper and lower surfaces of the same extension plate, and the screw rod is rotatably installed between the U-shaped frame and the same extension plate, and the lower surface of the screw rod is fixedly connected with the output end of the micro stepping motor.

[0011] In the technical scheme, the clamping device comprises a work-shaped block, two clamping blocks and a mountain-shaped magnet, the work-shaped block is slidably installed in the same U-shaped frame, and the work-shaped block is screw-connected and installed on the outer surface of the same screw rod, two transfer grooves are symmetrically formed in the right side of the work-shaped block, the two clamping blocks are rotatably installed in the corresponding transfer grooves, a clamping groove is formed between the opposite surfaces of the two clamping blocks, the top end of the syringe is clamped and installed between the two corresponding clamping grooves, the clamping block is made of iron, and the mountain-shaped magnet is clamped between the two clamping blocks.

[0012] In the technical scheme, the lower end of the syringe is provided with a disposable suction tube, an annular clamping groove is formed in the inner side wall of the disposable suction tube, and an annular ridge is integrally arranged on the outer surface of the lower end of the syringe.

[0013] In the technical scheme, the PLC comprehensive control panel is electrically connected with the electric telescopic rod, the angle adjusting motor, the micro stepping motor and the transmittance analyzer main body.

[0014] Compared with the prior art, the automatic food proteinase activity measuring device has the beneficial effects that:

[0015] The utility model discloses a lifting rotary liquid transfer frame realizes the accurate control to syringe, under the assistance of vertical lifting function, two syringes drive the piston rod of syringe to go up with the help of lifting device, respectively in two detection cup extraction protease liquid and bottom liquid, then lifting rotary liquid transfer frame removes two syringes from detection cup and makes it rotate to the light transmission cup above in proper order, liquid is injected into light transmission cup, then closes the light shield plate and creates the dark detection pool environment, opens the light source body, and light transmission cup is reached after the light of protease and bottom liquid mixed solution in light transmission cup and reaches the light transmission detection piece, and the light intensity attenuation is caused because mixed solution absorbs part light energy, and the light absorption spectrum is obtained by measuring the light absorption degree of mixed solution, and then the enzyme activity is calculated according to the comparison of absorbance and bottom liquid light absorption degree under specific wavelength. The device completes the extraction and removal of protease liquid and drop liquid to light transmission cup measurement in mechanical mode, can displace the syringe piston to the tiny scale when taking liquid, significantly improves the liquid taking precision, reduces the error, and guarantees the accuracy of food protease activity determination.

[0016] Two, the utility model discloses a syringe is inserted into the through -hole of extension board and is fixed with abutting bolt, and is adsorbed between two clamping blocks with mountain type magnet, and the piston rod of the syringe clamped between two clamping grooves is clamped and fixed, so that when replacing the syringe, it is more convenient and efficient. ACCURATELY DRAWING OUT LIQUID

[0017] Figure 1 It is the left view structural schematic drawing of the utility model.

[0018] Figure 2 It is the plan view structural schematic drawing of the utility model.

[0019] Figure 3 It is the clamping device explosion structural schematic drawing of the utility model.

[0020] Figure 4 It is the front view structural schematic drawing of the utility model.

[0021] Figure 5 It is the disposable pipette section structure schematic drawing of the utility model.

[0022] Figure 6 It is the carrier plate structure schematic drawing of the utility model.

[0023] Figures 1-6In the middle, wherein: 1, base; 11, placing groove; 111, detection cup; 12, detection pool; 121, cup body limiting post; 13, light transmission cup; 14, light shielding cover plate; 15, arc-shaped limiting block; 2, movable carrier; 21, electric telescopic rod; 22, carrier plate; 221, extension plate; 222, through opening; 223, abutting bolt; 23, angle adjusting motor; 3, lifting device; 31, micro stepping motor; 32, U-shaped frame; 33, screw rod; 4, clamping device; 41, I-shaped block; 411, adapter groove; 42, clamping block; 421, clamping groove; 43, mountain-shaped magnet; 5, syringe; 51, annular rib; 6, disposable pipette; 61, annular clamping groove; 7, light source body; 8, light transmission detection piece; 9, PLC comprehensive control panel; 10, transmittance analyzer main body. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0025] The front, back, left, right, up and down in the embodiments are described with reference to the base surface. Figure 1 Figures 1-6 The present application provides a technical solution:

[0026] ​The utility model provides an automatic food protein enzyme activity determination device, including base 1, fixedly installed with PLC comprehensive control panel 9 and transmittance analyzer main part 10 on base 1, two placing grooves 11 are set up in the right side of the upper surface of base 1, and the detecting cup 111 is placed in the placing groove 11, and the detecting pool 12 is set up in the left side of the upper surface of base 1, and two cup body limit posts 121 are fixedly installed in the detecting pool 12 left-right symmetry, and the light transmission cup 13 is placed in the two cup body limit posts 121, and the light source body 7 (the light source body 7 can be halogen tungsten lamp or light emitting diode LED light source) and the light transmission detection piece 8 (the light transmission detection piece 8 can be photodiode, and the electrical connection of light transmission detection piece 8 and transmittance analyzer main part 10 is prior art, and here does not make much explanation) are fixedly installed respectively on the front and rear sidewall in the detecting pool 12, the light transmission detection piece 8 is electrically connected with transmittance analyzer main part 10, the light shield cover plate 14 for covering the detecting pool 12 is rotatably installed on the left side of the upper surface of base 1, the lifting rotary liquid transfer frame is installed at the center position of the upper surface of base 1, two syringes 5 are fixedly installed at the right end of lifting rotary liquid transfer frame, two lifting devices 3 are installed on the right side of the upper surface of lifting rotary liquid transfer frame, the clamping device 4 is installed in the lifting device 3, the top end of syringe 5 is clamped and installed in the corresponding clamping device 4, and syringe 5 is located above the corresponding detecting cup 111;

[0027] It should be noted that, as shown in Figure 1 and Figure 4 The lifting rotary liquid transfer frame includes movable carrier 2, electric telescopic rod 21, carrier plate 22 and angle adjusting motor 23, the angle adjusting motor 23 is fixedly installed on the upper surface of base 1, and the output end of angle adjusting motor 23 is fixedly connected with the lower surface center of movable carrier 2, the electric telescopic rod 21 is fixedly installed on the upper surface of movable carrier 2, and the upper surface of electric telescopic rod 21 is fixedly connected with the lower surface of carrier plate 22, two extension plates 221 are integrally formed on the right side of carrier plate 22, the right end of extension plate 221 is provided with through hole 222, syringe 5 is inserted into the corresponding through hole 222, and the right surface of extension plate 221 is provided with resistance bolt 223 for fixing syringe 5 by screwing;

[0028] In use, first, the food protein enzyme liquid to be determined and the light-transmitting base liquid are respectively injected into the two detection cups 111, then the electric telescopic rod 21 is driven to retract, the lifting device 3, the clamping device 4 and the syringe 5 are driven to move downward by the carrier plate 22, the two syringes 5 are extended into the detection cups 111 to quantitatively draw the food protein enzyme liquid and the light-transmitting base liquid, then the movable carrier 2 is rotated by the angle adjusting motor 23, so that the syringes 5 are rotated to above the light-transmitting cup 13 in turn, then the piston rod of the syringe 5 is lowered by the lifting device 3, the drawn food protein enzyme liquid and light-transmitting base liquid are sequentially input into the light-transmitting cup 13 to mix, after mixing, the syringe 5 is moved upward and reset to the right position, finally the light-shielding cover plate 14 is flipped to the right side to cover the detection pool 12, the detection environment is provided, the light source body 7 is turned on, the light passes through the light-transmitting cup 13 and the protein enzyme and base liquid mixture in the light-transmitting cup 13 to reach the light-transmitting detection piece 8, the light collection data of the light-transmitting detection piece 8 is converted into an electric signal and transmitted to the transmittance analyzer main body 10 for analysis, because the mixed liquid absorbs part of the light energy to cause light intensity attenuation, the light absorption degree of the measured mixed liquid is obtained to obtain the absorption spectrum, and then the enzyme activity is calculated according to the comparison between the absorbance at a specific wavelength and the light absorption degree of the light-transmitting base liquid. Compared with the prior art, the present determination device can accurately sample the light-transmitting base liquid and the food protein enzyme, and can effectively ensure the accuracy of the determination data.

[0029] Specifically, as shown in the drawings, Figure 4 two arc-shaped limiting blocks 15 are symmetrically and fixedly installed on the upper surface of the base 1, and the movable carrier 2 is rotatably installed between the two arc-shaped limiting blocks 15.

[0030] As shown in the drawings, Figure 1 The lifting device 3 comprises a micro stepping motor 31, a U-shaped frame 32 and a screw rod 33, the screw rod 33 is a precision screw rod, the U-shaped frame 32 and the micro stepping motor 31 are fixedly installed on the upper and lower surfaces of the same side extension plate 221 respectively, the screw rod 33 is rotatably installed between the U-shaped frame 32 and the same side extension plate 221, and the lower surface of the screw rod 33 is fixedly connected with the output end of the micro stepping motor 31.

[0031] In combination with Figure 1 and Figure 3As shown, the clamping device 4 comprises a work-shaped block 41, two clamping blocks 42 and a mountain-shaped magnet 43. The work-shaped block 41 is slidingly installed in the U-shaped frame 32 on the same side, and is screwedly installed on the outer surface of the screw rod 33 on the same side. Two adapter grooves 411 are symmetrically formed in the right side of the work-shaped block 41. The two clamping blocks 42 are rotatably installed in the corresponding adapter grooves 411, respectively. A clamping groove 421 is formed between the opposite surfaces of the two clamping blocks 42. The top end of the syringe 5 is clamped and installed between the two corresponding clamping grooves 421. The clamping block 42 is made of iron. The mountain-shaped magnet 43 is clamped between the two clamping blocks 42 to fix the positions of the two clamping blocks 42. When the piston rod of the syringe 5 is not fixed, the mountain-shaped magnet 43 is removed from between the two clamping blocks 42. The clamping block 42 is rotated outward to make the top of the piston rod of the syringe 5 disengage from the clamping groove 421. When the syringe 5 is replaced, the tightening screw 223 is unscrewed to release the fixation of the syringe 5. The syringe 5 can be removed from the through hole 222 and replaced. Figure 6

[0032] When the piston rod of the syringe 5 needs to be adjusted, the micro stepping motor 31 drives the screw rod 33 to rotate, and the screw rod 33 drives the work-shaped block 41 to adjust the position up and down, so as to move the piston rod clamped in the two adapter grooves 411 up and down, achieving the purpose of liquid suction and discharge.

[0033] In order to reduce the replacement frequency of the syringe 5 during use, a disposable suction tube 6 is provided at the lower end of the syringe 5. An annular clamping groove 61 is formed in the inner side wall of the disposable suction tube 6. An annular ridge 51 is integrally formed on the outer surface of the lower end of the syringe 5. The outer surface of the annular ridge 51 is in close contact with the inner surface of the annular clamping groove 61. The disposable suction tube 6 is made of elastic plastic tube. When the syringe 5 is used to transfer food protein enzyme liquid and transparent base liquid, the liquid can be temporarily stored in the disposable suction tube 6. When the transfer amount is large, the liquid can be sucked in small amounts for multiple times. When different transparent base liquids need to be sucked for comparison and analysis, etc., the disposable suction tube 6 can be conveniently and quickly replaced with a new one for subsequent operation. This can not only ensure the accuracy and convenience of the liquid suction operation, but also can greatly avoid the problem that the syringe 5 needs to be frequently replaced due to frequent use, thereby improving the efficiency and economy of the entire operation process.

[0034] Finally, the PLC comprehensive control panel 9 is electrically connected with the electric telescopic rod 21, the angle adjusting motor 23, the micro stepping motor 31 and the transparency analyzer main body 10,

[0035] ​Before being put into formal use, the setting of the PLC comprehensive control panel 9 has strict and fine requirements. The specific numerical value of the rotation speed and the accurate setting value of the rotation angle of the angle adjustment motor 23, as well as the related operation parameters of the micro stepping motor 31, such as the rotation speed and the time duration of a single rotation, need to be comprehensively and meticulously pre-programmed. Through such a rigorous pre-programming process, the PLC comprehensive control panel 9 can accurately control the various components connected thereto according to the set program logic in the actual use scenario. This accurate control can ensure that the electric telescopic rod 21 moves according to the predetermined telescopic amplitude and speed, ensure that the angle adjustment motor 23 accurately reaches the set rotation speed and rotates to the expected angle, and also enable the micro stepping motor 31 to strictly perform the corresponding action according to the set rotation speed and single rotation time, thereby ensuring that the entire device can efficiently, accurately and stably operate in the related operation process such as transmittance analysis.

Claims

1. An automatic food protein enzyme activity determination device, comprising a base (1), a PLC integrated control panel (9) and a transmittance analyzer main body (10) are fixedly installed on the base (1), characterized in that, The upper surface of the base (1) is provided with two placing grooves (11) on the right side, and the detecting cups (111) are placed in the placing grooves (11); the upper surface of the base (1) is provided with a detecting pool (12) on the left side, and the two cup body limiting columns (121) are fixedly installed in the detecting pool (12) and symmetrically arranged; the light-transmitting cup (13) is clamped and placed between the two cup body limiting columns (121); the light source body (7) and the light-transmitting detecting piece (8) are fixedly installed on the front and rear sidewalls of the detecting pool (12), respectively; the light-transmitting detecting piece (8) is electrically connected with the light-transmittance analyzer main body (10); and the light-shielding cover plate (14) for covering the detecting pool (12) is rotatably installed on the left side of the upper surface of the base (1). The upper surface of the base (1) is provided with two placing grooves (11) on the right side, and the detecting cups (111) are placed in the placing grooves (11); the upper surface of the base (1) is provided with a detecting pool (12) on the left side, and the two cup body limiting columns (121) are fixedly installed in the detecting pool (12) and symmetrically arranged; the light-transmitting cup (13) is clamped and placed between the two cup body limiting columns (121); the light source body (7) and the light-transmitting detecting piece (8) are fixedly installed on the front and rear sidewalls of the detecting pool (12), respectively; the light-transmitting detecting piece (8) is electrically connected with the light-transmittance analyzer main body (10); and the light-shielding cover plate (14) for covering the detecting pool (12) is rotatably installed on the left side of the upper surface of the base (1).

2. The automated food protein enzyme activity assay device of claim 1, wherein, The lifting rotary liquid transfer frame comprises a movable carrier (2), an electric telescopic rod (21), a carrier plate (22) and an angle adjusting motor (23); the angle adjusting motor (23) is fixedly installed on the upper surface of the base (1), and the output end of the angle adjusting motor (23) is fixedly connected with the center of the lower surface of the movable carrier (2); the electric telescopic rod (21) is fixedly installed on the upper surface of the movable carrier (2), and the upper surface of the electric telescopic rod (21) is fixedly connected with the lower surface of the carrier plate (22); the right side of the carrier plate (22) is integrally provided with two extension plates (221); the right end of each of the extension plates (221) is provided with a through opening (222); the syringes (5) are insertedly installed in the corresponding through openings (222); and the right surface of each of the extension plates (221) is penetratedly and screwingly installed with a abutting bolt (223) for fixing the syringes (5).

3. An automated food protein enzyme activity assay device according to claim 2, wherein, The upper surface of the base (1) is provided with two placing grooves (11) on the right side, and the detecting cups (111) are placed in the placing grooves (11); the upper surface of the base (1) is provided with a detecting pool (12) on the left side, and the two cup body limiting columns (121) are fixedly installed in the detecting pool (12) and symmetrically arranged; the light-transmitting cup (13) is clamped and placed between the two cup body limiting columns (121); the light source body (7) and the light-transmitting detecting piece (8) are fixedly installed on the front and rear sidewalls of the detecting pool (12), respectively; the light-transmitting detecting piece (8) is electrically connected with the light-transmittance analyzer main body (10); and the light-shielding cover plate (14) for covering the detecting pool (12) is rotatably installed on the left side of the upper surface of the base (1).

4. The automated food protein enzyme activity assay device of claim 2, wherein, The lifting rotary liquid transfer frame comprises a movable carrier (2), an electric telescopic rod (21), a carrier plate (22) and an angle adjusting motor (23); the angle adjusting motor (23) is fixedly installed on the upper surface of the base (1), and the output end of the angle adjusting motor (23) is fixedly connected with the center of the lower surface of the movable carrier (2); the electric telescopic rod (21) is fixedly installed on the upper surface of the movable carrier (2), and the upper surface of the electric telescopic rod (21) is fixedly connected with the lower surface of the carrier plate (22); the right side of the carrier plate (22) is integrally provided with two extension plates (221); the right end of each of the extension plates (221) is provided with a through opening (222); the syringes (5) are insertedly installed in the corresponding through openings (222); and the right surface of each of the extension plates (221) is penetratedly and screwingly installed with a abutting bolt (223) for fixing the syringes (5).

5. An automated food protein enzyme activity assay device according to claim 4, wherein, The clamping devices (4) each comprise a work-shaped block (41), two clamping blocks (42) and a mountain-shaped magnet (43), the work-shaped block (41) is slidingly installed in the same side U-shaped frame (32), and the work-shaped block (41) is screwedly installed on the outer surface of the same side screw rod (33), the right side of the work-shaped block (41) is symmetrically provided with two adapter grooves (411), the two clamping blocks (42) are rotatably installed in the corresponding adapter grooves (411) respectively, and the opposite surfaces of the two clamping blocks (42) are both provided with clamping grooves (421), the top end of the syringe (5) is clamped and installed between the two corresponding clamping grooves (421), the clamping blocks (42) are iron pieces, and the mountain-shaped magnet (43) is clamped between the two clamping blocks (42).

6. The automated food protein enzyme activity assay device of claim 1, wherein, The lower end of the syringe (5) is provided with a disposable suction tube (6), the inner side wall of the disposable suction tube (6) is provided with an annular clamping groove (61), and the lower end of the syringe (5) is integrally provided with an annular ridge (51), and the outer surface of the annular ridge (51) is attached to the inner surface of the annular clamping groove (61).

7. The automated food protein enzyme activity assay device of claim 5, wherein, The PLC comprehensive control panel (9) is electrically connected with the electric telescopic rod (21), the angle adjusting motor (23), the micro stepping motor (31) and the transmittance analyzer main body (10).

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