Automatic sperm smearing and dyeing device

Abstract

The utility model provides an automatic sperm smear staining device, which relates to the technical field of experimental smear devices and comprises a slide frame, a smear frame, a solution box and a workbench, the slide frame is positioned above the solution box, the solution box is arranged at the top of the workbench, the smear frame is arranged at the top of the workbench, the slide frame is fixedly arranged on the outer side of a middle rotating frame, and the middle rotating frame is fixedly arranged on the outer side of the middle rotating frame. The middle rotating frame is arranged on the outer side of the inner lifting frame in a circumferential rotating mode, the inner lifting frame is in threaded connection with the electric lifting screw, the inner lifting frame and the middle lifting rod vertically penetrate through and are in sliding connection, and a driving motor is fixedly installed at the top of the inner lifting frame. The slide on the smear frame realizes smear operation on seminal fluid on the slide on the top of the bearing table, the manual smear process of personnel is replaced, inconsistency and errors caused by manual smear of the personnel are avoided, and the problem that the smear quality is unstable due to the fact that parameters such as the smear speed and the smear pulling angle are difficult to accurately control is solved.

Description

Technical Field

[0001] This utility model relates to the technical field of laboratory smear devices, and in particular to an automatic sperm smear staining device. Background Technology

[0002] Sperm morphology analysis is an important means of assessing male fertility. It is mainly used to detect the proportion of abnormal sperm in semen samples, and its accuracy depends on the quality of semen smears.

[0003] Currently, the preparation and staining of semen smears largely rely on manual operation, which presents the following problems: ① During the preparation process, parameters such as smearing speed and slide angle are difficult to control precisely, leading to unstable smear quality and operational errors between different personnel and batches, ultimately affecting the accurate interpretation of sperm morphology; ② The staining process requires repeatedly placing the smear into different reagents, which is cumbersome and inefficient. Furthermore, the staining reagents are volatile and toxic, and long-term exposure may harm the health of laboratory personnel. Therefore, there is an urgent need for a device that can automate sperm smear staining to improve detection efficiency, ensure result accuracy, and protect the safety of laboratory personnel. Summary of the Invention

[0004] This disclosure relates to an automated sperm smear staining device. A first telescopic cylinder pushes a connecting frame and a smear rack to move horizontally. The slides on the smear rack smear the semen on the top slide of the support platform, replacing the manual smearing process and eliminating the inconsistencies and errors caused by manual smearing. The smear rack's support angle is adjusted through the cooperation of a rear support screw and a rear support tube. Simultaneously, the first telescopic cylinder controls the moving speed of the smear rack, achieving precise control of parameters such as smearing speed and slide pulling angle, ensuring stable smear quality, and effectively avoiding operational errors between different operators and batches, which could lead to errors in sperm morphology interpretation.

[0005] In a first aspect, this disclosure provides an automated sperm smear staining device, specifically comprising: a slide holder, a smear holder, a solution box, and a worktable. The slide holder is located above the solution box, the solution box is disposed on the top of the worktable, the smear holder is disposed on the top of the worktable, the slide holder is fixedly disposed outside a central rotating frame, the central rotating frame is circumferentially rotatable outside an inner lifting frame, the inner lifting frame is screwed to an electric lifting screw, the inner lifting frame and the central lifting screw are perpendicularly connected and slidably connected, and a drive motor is fixedly installed on the top of the inner lifting frame.

[0006] In at least some embodiments, the upper motor and the middle lifting rod of the electric lifting screw are fixed, the lower end of the electric lifting screw is rotatably connected to the worktable, the main shaft of the drive motor meshes with the external gear block of the middle rotating frame through gears, the lower end of the middle lifting rod is fixedly connected to the worktable, the thread of the electric lifting screw guides the inner lifting frame, the middle rotating frame and the slide holder to move vertically downward, the slide holder enters the solution box for immersion of the slides, and the slide holder, as the middle rotating frame rotates and rises, sequentially performs the immersion and staining process on the multiple solution boxes below.

[0007] In at least some embodiments, slidable side clamps are symmetrically arranged on both sides of the smear holder, and a central connecting tension spring is fixed between the side clamps on both sides of the smear holder. The rear side of the smear holder is rotatably connected to the first end of the rear support screw shaft. The rear section of the rear support screw is screwed to the rear support tube. The tail end of the rear support tube is rotatably connected to the lower connecting frame. The lower connecting frame shaft is rotatably mounted on the top of the lower moving slide. The lower moving slide is fixedly connected to the telescopic rod of the first telescopic cylinder. The support angle of the smear holder is adjusted by the cooperation of the rear support screw and the rear support tube.

[0008] In at least some embodiments, the lower connecting frame is horizontally slidably disposed on the top of the worktable, the first telescopic cylinder is fixed on the top of the worktable, the middle part of the smear holder is rotatably connected to the worktable pivot, the first telescopic cylinder pushes the lower connecting frame and the smear holder to move horizontally, and the slide of the smear holder performs a smearing operation on the semen on the slide at the top of the support platform.

[0009] In at least some embodiments, the top of the solution box is provided with a slidable and sealable upper sealing cover, and the upper sealing cover is fixedly connected to the telescopic rod of the second telescopic cylinder.

[0010] In at least some embodiments, the second telescopic cylinder is fixedly installed on the top of the workbench. When the second telescopic cylinder extends, it pushes the upper sealing cover away from the solution box. At this time, the top of the solution box is in an open state. When the second telescopic cylinder shortens, it drives the upper sealing cover to move and cover the top of the solution box. The solution box is in a sealed state at this time. The sealed solution box reduces the evaporation of the solution inside the solution box.

[0011] In at least some embodiments, the worktable has a support platform fixed to the front side of the smear holder, and the worktable has an upper sliding slot arranged in a ring array around the central lifting rod, with the upper sliding slot and the solution box slidingly connected.

[0012] This invention provides an automated sperm smear staining device, which has the following beneficial effects:

[0013] The first telescopic cylinder pushes the connecting frame and the smear frame to move horizontally. The slides of the smear frame are used to smear the semen on the slide at the top of the support platform, replacing the manual smearing process and eliminating the inconsistencies and errors caused by manual smearing. The support angle of the smear frame is adjusted by the cooperation of the rear support screw and the rear support tube. At the same time, the first telescopic cylinder controls the moving speed of the smear frame, realizing precise control of parameters such as smearing speed and slide pulling angle, ensuring the stability of smear quality, and effectively avoiding the problem of error in sperm morphology interpretation caused by operational errors between different workers and batches. This provides reliable smear samples for sperm morphology analysis.

[0014] The slide holder holds the prepared slides. As the central rotating frame rotates and rises, the slide holder sequentially soaks and stains multiple solution boxes below, precisely controlling the soaking time to improve detection efficiency and ensure consistency of staining results, thereby guaranteeing the accuracy of the test results. The second telescopic cylinder drives the upper sealing cover to seal and open the solution boxes, reducing the evaporation of the solution inside the boxes, lowering the concentration of harmful gases in the experimental environment, providing a safe environment for laboratory personnel, and protecting their health. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings of the embodiments will be briefly described below.

[0016] The accompanying drawings described below are only related to some embodiments of the present invention and are not intended to limit the scope of the present invention.

[0017] In the attached diagram:

[0018] Figure 1 A schematic diagram of the overall structure of this application is shown;

[0019] Figure 2 A schematic diagram of the coating holder structure of this application is shown;

[0020] Figure 3 A schematic diagram of the slide holder structure of this application is shown;

[0021] Figure 4 A schematic diagram of the solution box structure in the open state of this application is shown;

[0022] Figure 5 This diagram shows the structure of the slide holder in the solution box of this application in a structural state;

[0023] Figure 6 This invention provides a structural schematic diagram of the inner lifting frame and the middle lifting rod in their separated states.

[0024] List of reference numerals

[0025] 1. Slide holder; 101. Central rotating frame; 102. Inner lifting frame; 103. Electric lifting screw; 104. Central lifting rod; 105. Drive motor;

[0026] 2. Smear holder; 201. Side clamping block; 202. Middle connecting tension spring; 203. Rear support screw; 204. Rear support screw tube; 205. Lower connecting frame; 206. Lower moving slide; 207. First telescopic cylinder;

[0027] 3. Solution box; 301. Upper sealing cap; 302. Second telescopic cylinder;

[0028] 4. Workbench; 401. Upper sliding slot; 402. Support platform. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the described embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0030] Example 1: Please refer to Figures 1 to 6 :

[0031] This invention discloses an automatic sperm smear staining device, comprising: a slide holder 1, a smear holder 2, a solution container 3, and a worktable 4. The slide holder 1 is fixedly disposed outside a central rotating frame 101, which is circumferentially rotatable outside an inner lifting frame 102. The inner lifting frame 102 is screwed to an electric lifting screw 103, and the inner lifting frame 102 and the central lifting rod 104 are vertically connected and slidably connected. A drive motor 105 is fixedly installed on the top of the inner lifting frame 102. The slide holder 1 is located above the solution container 3. The top of the solution container 3 is provided with a slidable and sealable upper sealing cover 301, which is fixedly connected to the telescopic rod of a second telescopic cylinder 302. The second telescopic cylinder 302 is fixedly connected to the worktable 4, and the solution container 3 is disposed on the top of the worktable 4. A support platform 402 is fixedly disposed on the front side of the smear holder 2 on the worktable 4. The worktable 4 is provided with an upper sliding slot 4 arranged in a ring around the central lifting rod 104. 01. The upper sliding slot 401 and the solution box 3 are slidably connected. The upper sliding slot 401 can also be internally fitted with an absorbent sponge to absorb excess solution from the slide holder 1. The top of the worktable 4 is equipped with a smear holder 2. Sliding side clamps 201 are symmetrically arranged on both sides of the smear holder 2. A central connecting tension spring 202 is fixed between the side clamps 201 on both sides of the smear holder 2. The rear side of the smear holder 2 is rotatably connected to the first end of the rear support screw 203. The rear section of the rear support screw 203 is screwed to the rear support screw tube 204. The tail end of the rear support screw tube 204 is circumferentially connected to the lower connecting frame 205. The lower connecting frame 205 is rotatably mounted on the top of the lower moving slide 206. The lower moving slide 206 is fixedly connected to the telescopic rod of the first telescopic cylinder 207. The smear holder 2 adjusts its support angle through the cooperation of the rear support screw 203 and the rear support screw tube 204 to achieve precise control of the slide pulling angle.

[0032] In this embodiment, the upper motor and the middle lifting rod 104 of the electric lifting screw 103 are fixed, and the lower end of the electric lifting screw 103 is rotatably connected to the worktable 4. The main shaft of the drive motor 105 meshes with the external gear block of the middle rotating frame 101 through gears. The lower end of the middle lifting rod 104 is fixedly connected to the worktable 4. The thread of the electric lifting screw 103 guides the inner lifting frame 102, the middle rotating frame 101, and the slide holder 1 to move vertically downward. The slide holder 1 enters the solution box 3 for slide immersion. The slide holder 1 rotates and rises with the middle rotating frame 101 to sequentially perform the immersion and staining process on the multiple solution boxes 3 below, thereby improving detection efficiency, ensuring the consistency of staining results, and reducing manual operation.

[0033] In this embodiment, the lower connecting frame 205 is horizontally slidably disposed on the top of the workbench 4, the first telescopic cylinder 207 is fixed on the top of the workbench 4, the middle part of the smear holder 2 is rotatably connected to the rotating shaft of the workbench 4, and the first telescopic cylinder 207 pushes the lower connecting frame 205 and the smear holder 2 to move horizontally, so that the glass slide of the smear holder 2 can perform smearing operation on the semen on the glass slide on the top of the support platform 402, eliminating the manual smearing process and avoiding the inconsistency and error problems caused by manual smearing.

[0034] In this embodiment, the second telescopic cylinder 302 is fixedly installed on the top of the workbench 4. When the second telescopic cylinder 302 extends, it pushes the upper sealing cover 301 to move away from the solution box 3. At this time, the top of the solution box 3 is in an open state. When the second telescopic cylinder 302 shortens, it drives the upper sealing cover 301 to move and cover the top of the solution box 3. The solution box 3 is in a sealed state at this time. After the solution box 3 is sealed, the evaporation of the solution inside the solution box 3 is reduced, and the harmful gases generated by the evaporation of the liquid inside the solution box 3 in the experimental environment are reduced, making the experimental environment safer.

[0035] In Example 2, based on Example 1, the workbench 4 is further equipped with a turbidity measurement module. By comparing the turbidity display image of the standard sample, the concentration of sperm used for smear is ensured to be within a suitable concentration range. The turbidity measurement module includes a light source, a cuvette, and a control image or sample, and is used to measure and adjust the sperm concentration to ensure that the sperm concentration is at a uniform level.

[0036] The working principle of this embodiment is as follows: A glass slide is placed on the top of the support platform 402, and a glass slide is placed at an angle on the top of the smear holder 2. The connecting spring 202 pulls the side clamping block 201 to clamp the middle glass slide. The lower part of the glass slide on the top of the smear holder 2 is at the top of the glass slide on the top of the support platform 402. The first telescopic cylinder 207 pushes the connecting frame 205 and the smear holder 2 to move horizontally. The glass slide on the smear holder 2 performs a smearing operation on the semen on the glass slide on the top of the support platform 402, eliminating the need for manual smearing, reducing the labor intensity of personnel, and eliminating the inconsistency and error problems caused by manual smearing. The rear support screw tube 204 rotates circumferentially, and the thread of the rear support screw tube 204 guides the rear support screw 203 to move axially. The rear support screw 203 and rear support tube 204 extend to support the slide holder 2 to rotate around the connecting shaft between the slide holder 2 and the lower moving slide 206 to adjust the angle. The support angle of the slide holder 2 is adjusted by the cooperation of the rear support screw 203 and rear support tube 204. The slide holder 2 is generally kept at a 40-degree tilt. At the same time, the extension and retraction of the first telescopic cylinder 207 controls the moving speed of the slide holder 2 and the glass slide, realizing precise control of parameters such as the slide speed and the slide pulling angle, ensuring the stability of the slide quality, effectively avoiding the problem of error in sperm morphology interpretation caused by operational errors in parameters such as the slide speed and the slide pulling angle between different workers and batches, and providing more reliable slide samples for sperm morphology analysis.

[0037] Slides after smearing are placed inside slide holder 1. Drive motor 105, through gear meshing, rotates the rotating frame 101 and slide holder 1 to above solution container 3, which remains open. The electric lifting screw 103 guides the inner lifting frame 102, the rotating frame 101, and slide holder 1 vertically downwards, allowing slide holder 1 to enter solution container 3 for slide immersion. As the rotating frame 101 rotates and rises, slide holder 1 sequentially immerses and stains multiple solution containers 3 below. The immersion time is precisely controlled via a PLC module, improving detection efficiency and ensuring staining accuracy. To ensure the consistency of color results and thus the accuracy of the test results, when the slide holder 1 is above the solution box 3, the extension rod of the second telescopic cylinder 302 extends, causing the upper sealing cover 301 to move away from the solution box 3, thereby opening the solution box 3. When the extension rod of the second telescopic cylinder 302 shortens, it causes the upper sealing cover 301 to move and cover the solution box 3, thereby closing and sealing the solution box 3. After the solution box 3 is closed and sealed, the evaporation of the solution inside the solution box 3 is reduced, and the harmful gases generated by the evaporation of the solution box 3 in the experimental environment are reduced, providing a safe environment for the experimental personnel and helping to protect the health of the experimental personnel.

[0038] The following points should be noted in this article:

[0039] 1. The accompanying drawings of the embodiments disclosed herein only involve structures relevant to the embodiments disclosed herein; other structures may refer to general designs.

[0040] 2. Where there is no conflict, the embodiments of this disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

[0041] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this disclosure should be included within the scope of protection of this disclosure. Therefore, the scope of protection of this disclosure should be determined by the scope of the claims.

Claims

1. An automatic sperm smear staining device, comprising: The utility model provides a slide rack (1), smear rack (2), solution box (3) and workbench (4), its characterized in be that the slide rack (1) is located the upper solution box (3), and the solution box (3) is arranged on the top of workbench (4), and the top of workbench (4) is provided with smear rack (2), and the slide rack (1) is fixedly arranged outside the middle rotating frame (101), and the middle rotating frame (101) is circumferentially rotatably arranged outside the inner lifting frame (102), and the inner lifting frame (102) and electric lifting screw (103) are screwed, and the inner lifting frame (102) and middle lifting rod (104) are vertically penetrated and keep sliding connection, and the top of inner lifting frame (102) is fixedly installed with drive motor (105).

2. The automatic sperm smear staining device according to claim 1, wherein The upper end motor of electric lifting screw (103) and middle lifting rod (104) are fixed, and the lower end of electric lifting screw (103) and workbench (4) are rotatably connected, the main shaft of drive motor (105) is engaged with middle rotating frame (101) through gear, and the lower end of middle lifting rod (104) and workbench (4) are fixedly connected.

3. The automatic sperm smear staining device according to claim 1, wherein The both sides of smear rack (2) are symmetrically provided with slidable side clamping blocks (201), the middle connecting tension spring (202) is fixed between the side clamping blocks (201) of the both sides of smear rack (2), the rear side of smear rack (2) and the first end of rear support screw (203) are rotatably connected in shaft hole fit, the rear section of rear support screw (203) and rear support screw pipe (204) are screwed, the tail end of rear support screw pipe (204) and lower connecting frame (205) are circumferentially rotatably connected, lower connecting frame (205) is rotatably arranged on the top of lower moving slide (206) in shaft hole fit, and lower moving slide (206) and the telescopic rod of first telescopic cylinder (207) are fixedly connected.

4. The automatic sperm smear staining device according to claim 3, wherein The lower connecting frame (205) is horizontally slidably arranged on the top of workbench (4), the first telescopic cylinder (207) is fixed on the top of workbench (4), and the middle part of smear rack (2) and workbench (4) are rotatably connected in shaft hole fit.

5. The automatic sperm smear staining device according to claim 1, wherein The top of solution box (3) is provided with slidable seal upper sealing cover (301), and the telescopic rod of second telescopic cylinder (302) and upper sealing cover (301) are fixedly connected.

6. The automatic sperm smear staining device according to claim 5, wherein The second telescopic cylinder (302) is fixedly arranged on the top of workbench (4), the second telescopic cylinder (302) is elongated to push upper sealing cover (301) to move away from solution box (3), at this time, the top of solution box (3) is in open state, the second telescopic cylinder (302) is shortened to drive upper sealing cover (301) to move and cover on the top of solution box (3), and solution box (3) is in sealed state.

7. The automatic sperm smear staining device according to claim 1, wherein The workbench (4) is fixed with bearing table (402) on the front side of smear rack (2), and the workbench (4) is provided with upper sliding slot (401) in annular array around the middle lifting rod (104), and the upper sliding slot (401) and solution box (3) are slidingly connected.

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