Liquid chromatographic column tube suitable for blood sample injection

Abstract

The utility model relates to the technical field of chromatographic column tubes, and discloses a liquid chromatographic column tube suitable for blood sample introduction, which comprises a column tube body, porous titanium sieve plates are arranged at two ends of the column tube body, threads are arranged at two ends of the column tube body, the average aperture of each porous titanium sieve plate is 20-50 microns, the porous titanium sieve plates are made of titanium, and the diameter of each porous titanium sieve plate is 20-50 microns. The porous titanium sieve plate is a porous structure wafer formed by sintering titanium powder or titanium fibers at high temperature, the outer wall of the porous titanium sieve plate is wrapped with the annular polyether-ether-ketone, and the outer wall of the annular polyether-ether-ketone is round. According to the utility model, adsorption of protein and the like in a blood sample is reduced through biological inertness of a titanium material so as to reduce the blocking risk, and the porous structure of the titanium sieve plate and the straight-through round holes in the column tube synergistically optimize the flow of the blood sample and a mobile phase; a stable environment and guarantee are provided for separating the blood sample in the column according to the action difference of each component and a stationary phase and analyzing and acquiring component information through a detector.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of chromatographic column tube especially relates to a liquid chromatographic column tube suitable for blood sample injection. BACKGROUND

[0002] In the field of analytical chemistry, liquid chromatography is a very important analysis technology, which can separate and detect trace substances in various samples. With the development of technology and social progress, people pay more and more attention to their own health, and various clinical detection technologies for risk monitoring and disease diagnosis have emerged as the times require. The samples detected in this scene are mainly blood samples. Blood samples contain proteins, fats, sugars, inorganic salts and other components, which can interfere with the detection of trace substances (such as hormones), so blood samples need to be pretreated to remove these interfering components and extract and enrich trace substances. Common pretreatment methods include protein precipitation, liquid-liquid extraction, solid-phase extraction, etc. However, these methods are offline operations. After the blood sample is treated, a specific volume of liquid is obtained, which is then transferred to the subsequent detection equipment (liquid chromatography, liquid chromatography mass spectrometry, etc.) for quantitative detection. This process is relatively cumbersome, and only a part of the specific volume of liquid is detected, which means that although the trace substances in the blood sample are extracted, they are not all detected, resulting in insufficient sensitivity of the detection method.

[0003] Online sample pretreatment can effectively solve this problem. It uses a liquid chromatographic column filled with a large particle size particle filler, and drives a certain mobile phase through an online processing device to transport the blood sample to the column. The trace substances in the blood sample are adsorbed on the filler in the column, and then another mobile phase is used to wash the trace substances from the filler in the column and transport them to the subsequent detection equipment for qualitative and quantitative detection. This online sample pretreatment technology has high automation, and all the trace substances obtained by processing enter the subsequent detection equipment, improving the detection sensitivity. However, online sample pretreatment often encounters the problem of chromatographic column tube blockage. The main reason for this problem is that proteins, fats (including phospholipids) in the blood sample are easily adsorbed by the screen plate of the chromatographic column tube, and after multiple online sample pretreatments, the pores of the screen plate are blocked by accumulated substances. SUMMARY

[0004] To make up for the above shortcomings, the utility model provides a liquid chromatographic column tube suitable for blood sample injection, aiming at improving the problem that in the traditional chromatographic column tube, proteins, fats, etc. in the blood sample are easily adsorbed by the screen plate of the chromatographic column tube, and after multiple online sample pretreatments, the pores of the screen plate are blocked by accumulated substances.

[0005] In order to achieve the above object, the utility model discloses the following technical scheme: a liquid chromatography column pipe suitable for blood sample sampling, including column pipe body, the both ends of column pipe body are provided with porous titanium sieve plate, the both ends of column pipe body are provided with screw threads, the average aperture of porous titanium sieve plate is 20-50mu m titanium sieve plate.

[0006] Further, the average aperture of the porous titanium sieve plate is 20-50mu m.

[0007] Further, the material of the porous titanium sieve plate is titanium.

[0008] Further, the porous titanium sieve plate is a porous structure disc formed by high-temperature sintering of titanium powder or titanium fiber.

[0009] Further, the outer wall of the porous titanium sieve plate is wrapped with the toroidal polyether ether ketone, the outer wall of the toroidal polyether ether ketone is circular, the outer diameter of the toroidal polyether ether ketone is same with the outer diameter of the column pipe body.

[0010] Further, the porous titanium sieve plate is fixed on the both ends of the column pipe body through the nut, the nut and the column pipe body are fixed through the screw threads, and the toroidal polyether ether ketone of the outer wall of the porous titanium sieve plate is clamped.

[0011] Further, the material of the column pipe body is stainless steel or titanium.

[0012] Further, a straight-through circular hole is formed in the column pipe (1), and the inner diameter of the circular hole is 0.3-3mm.

[0013] Further, the column pipe body is wound with conductive silica gel ring.

[0014] The utility model has the advantages of:

[0015] In the utility model, first, the porous titanium sieve plate with specific aperture is arranged at the both ends of the column pipe body, the biological inertia of titanium material is utilized to reduce the adsorption of protein in the blood sample and reduce the risk of blockage, the porous structure and the straight-through circular hole inside are cooperatively optimized to make the blood sample and the mobile phase flow, the polyether ether ketone (ring material) of the outer wall of the titanium sieve plate is clamped when the nut and the column pipe are fixed through the screw threads, which not only protects the sieve plate but also ensures that the sieve plate is tightly fixed at the both ends of the column pipe, the material of the column pipe body is stainless steel or titanium, which provides a stable environment and guarantee for realizing separation of the blood sample in the column according to the difference between components and stationary phase and obtaining component information through the detector. ACCURACY

[0016] Figure 1 It is the perspective view of the liquid chromatography column pipe of the utility model;

[0017] Figure 2 It is the column pipe body structure schematic view of the liquid chromatography column pipe of the utility model;

[0018] Figure 3 This is a schematic diagram of the porous titanium sieve plate structure of the liquid chromatography column of this utility model.

[0019] Figure 4 A three-dimensional view of the liquid chromatography column of this invention (including the silica ring).

[0020] Legend:

[0021] 1. Column tube body; 2. Thread; 3. Nut; 4. Porous titanium sieve plate; 5. Circular polyetheretherketone. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Reference Figures 1-2 This utility model provides an embodiment of a liquid chromatography column suitable for blood sample injection, comprising a column body 1, with porous titanium sieve plates 4 disposed at both ends of the column body 1, and threads 2 disposed at both ends of the column body 1. The porous titanium sieve plates 4 are titanium sieve plates with an average pore size of 20-50 μm, and are made of titanium. The porous titanium sieve plates 4 are porous circular discs formed by high-temperature sintering of titanium powder or titanium fibers. Figure 3 The circular holes on the surface are simulated micropores. The outer wall of the porous titanium sieve plate 4 is wrapped with a circular polyether ether ketone 5. The outer wall of the circular polyether ether ketone 5 is circular. The outer diameter of the circular polyether ether ketone 5 is the same as the outer diameter of the column tube body 1. The porous titanium sieve plate 4 is fixed to both ends of the column tube body 1 by nuts 3. The nuts 3 and the column tube body 1 are fixed by threads 2, and the circular polyether ether ketone 5 on the outer wall of the porous titanium sieve plate 4 is clamped. The column tube body 1 is made of stainless steel or titanium. A straight circular hole is opened inside the column tube (1). The inner diameter of the circular hole is 0.3-3mm.

[0024] Specifically, a titanium sieve plate with an average pore size of 30μm and a diameter of 3mm is wrapped in a ring-shaped polyether ether ketone (PEEK) material with an outer diameter of 6.35mm. Two of these sieve plates are placed at both ends of a stainless steel column tube with an inner diameter of 2.1mm, an outer diameter of 6.35mm, and a length of 100mm. Nuts 3 are added to each end and tightened to form a liquid chromatography column tube with an inner diameter of 2.1mm and a length of 100mm.

[0025] A 2mm diameter titanium sieve plate with an average pore size of 50μm is wrapped in a circular ring-shaped polyether ether ketone (PEEK) material with an outer diameter of 6.35mm. Two of the sieve plates are placed at the two ends of a stainless steel column tube with an inner diameter of 1mm, an outer diameter of 6.35mm, and a length of 50mm. Screw caps 3 are added to the two ends, and the column tube is tightened to form a 1mm (inner diameter) * 50mm (length) liquid chromatography column tube.

[0026] A 1mm diameter titanium sieve plate with an average pore size of 20μm is wrapped in a circular ring-shaped polyether ether ketone (PEEK) material with an outer diameter of 6.35mm. Two of the sieve plates are placed at the two ends of a stainless steel column tube with an inner diameter of 0.5mm, an outer diameter of 6.35mm, and a length of 50mm. Screw caps 3 are added to the two ends, and the column tube is tightened to form a 0.5mm (inner diameter) * 50mm (length) liquid chromatography column tube.

[0027] Working principle: The blood sample is first introduced into the column tube body 1. The porous titanium sieve plates 4 at both ends of the column tube body 1 play a key role, with an average pore size of 20-50μm. This specific pore size allows the target analyte in the blood sample and the mobile phase to pass through smoothly, while effectively blocking larger particulate impurities in the blood sample from entering the column tube interior and affecting the normal operation of the chromatography column. The porous titanium sieve plates 4 are made of a porous structure disc formed by high-temperature sintering of titanium powder or titanium fibers, and are made of titanium, which is biologically inert and can reduce the adsorption of biological macromolecules such as proteins and phospholipids in the blood sample by the column tube, thereby reducing the risk of column tube blockage and ensuring long-term stable operation of the chromatography column.

[0028] The outer wall of the porous titanium sieve plate 4 is wrapped with a circular ring-shaped polyether ether ketone 5. The circular ring-shaped polyether ether ketone (ring material) 5 can protect the porous titanium sieve plate 4 from damage by external forces during installation or use. When the screw cap 3 is fixed to the column tube body 1 by the threads 2 and clamps the circular ring-shaped polyether ether ketone 5, it ensures the tight fixation of the porous titanium sieve plate 4 at both ends of the column tube body 1, preventing it from shifting or loosening, and thus ensuring the formation of a stable separation area between the sieve plates at both ends of the column tube and the interior space of the column tube.

[0029] The conductive silicone ring can adjust the temperature during column backwashing to ensure that the column interior is clean.

[0030] The column tube body 1 is made of stainless steel or titanium, which can provide a stable physical environment for the internal chromatographic separation process. The inner diameter of the built-in circular hole is 0.3-3mm, and the porous titanium sieve plate 4 is uniformly distributed with micropores, which further optimizes the flow characteristics of the blood sample and the mobile phase in the column tube. The blood sample can be effectively separated according to the difference in the interaction of each component with the stationary phase under the push of the mobile phase, so as to accurately obtain the information of each component in the blood sample, and the possibility of sieve plate blockage is reduced. This makes the column tube maintain good permeability for a long time, reduces the damage or performance degradation of the column tube caused by blockage, and significantly prolongs the service life of the liquid chromatography column tube.

[0031] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the foregoing detailed description of the present application is made with reference to the foregoing embodiments, for those skilled in the art, it still can be modified to the technical solutions recorded in the foregoing embodiments, or equivalent replacement of some of the technical features, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application, should be included in the protection scope of the present application.

Claims

1. A liquid chromatography column tube suitable for blood sample injection, comprising a column tube body (1), characterized in that: The column tube body (1) is provided with a porous titanium sieve plate (4) at both ends, and is provided with a thread (2) at both ends.

2. A liquid chromatography column tube suitable for blood sample injection according to claim 1, characterized in that: The average pore diameter of the porous titanium sieve plate (4) is 20-50 microns.

3. A liquid chromatography column tube suitable for blood sample injection according to claim 2, characterized in that: The material of the porous titanium sieve plate (4) is titanium.

4. A liquid chromatography column tube suitable for blood sample injection according to claim 2, wherein: The porous titanium sieve plate (4) is a porous structure disc made of titanium powder or titanium fiber through high-temperature sintering.

5. A liquid chromatography column tube suitable for blood sample injection according to claim 1, wherein: The outer wall of the porous titanium sieve plate (4) is wrapped with a circular ring-shaped polyether ether ketone (5), the outer wall of the circular ring-shaped polyether ether ketone (5) is circular, the outer diameter of the circular ring-shaped polyether ether ketone (5) is the same as the outer diameter of the column tube body (1).

6. A liquid chromatography column tube suitable for blood sample injection according to claim 5, characterized in that: The porous titanium sieve plate (4) is fixed at both ends of the column tube body (1) through a screw cap (3), the screw cap (3) and the column tube body (1) are fixed through the thread (2), and the circular ring-shaped polyether ether ketone (5) on the outer wall of the porous titanium sieve plate (4) is clamped.

7. A liquid chromatography column tube suitable for blood sample injection according to claim 6, characterized in that: The material of the column tube body (1) is stainless steel or titanium.

8. A liquid chromatography column tube suitable for blood sample injection according to claim 7, characterized in that: A straight-through circular hole is formed in the column tube body (1), and the inner diameter of the circular hole is 0.3-3 mm.

9. The liquid chromatography column tube suitable for blood sample injection of claim 1, wherein: The outer edge of the column tube body is wrapped with a conductive silica gel ring.

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