Chemiluminescence immunoassay device and magnetic washing structure thereof

Abstract

The utility model relates to a chemiluminescence immunoassay device and a magnetic washing structure thereof, and effectively solves the problems that the accuracy of a detection result is influenced because the magnetic field intensity of a permanent magnet group cannot be adjusted because a compound is separated from a liquid phase by mostly adopting a magnetic field generated by the permanent magnet group in the conventional magnetic separation washing device. Comprising a liquid injection assembly arranged on a supporting frame, the liquid injection assembly comprises a liquid injection fixing plate coaxially fixed to the supporting frame, and a plurality of liquid injection needles are arranged on the liquid injection fixing plate; the magnetic bead cleaning device is ingenious in structure and convenient to use, compatibility of magnetic bead cleaning to different magnetic beads is achieved through the arrangement of the electromagnetic iron core, the magnetic attraction block, the coil and the like, the problem that cleaning is not thorough due to the fact that the magnetic bead attraction force is large when the magnetic field intensity is high is solved, meanwhile, the magnetic attraction block does not demagnetize due to time increase, the magnetic field intensity is weakened, and the magnetic bead cleaning efficiency is improved. Therefore, the accuracy of the detection result is greatly improved.

Description

Technical Field

[0001] This utility model relates to the field of chemiluminescence immunoassay technology, and in particular to a chemiluminescence immunoassay device and its magnetic washing structure. Background Technology

[0002] Chemiluminescence immunoassay is an ultra-micro analysis technique that combines chemiluminescence with an immune reaction. It uses a chemiluminescence or bioluminescence system as an indicator of antigen-antibody reaction, and the concentration of antigen or antibody can be quantitatively detected based on the luminescence intensity of the luminescent material.

[0003] In practical applications, antigens or antibodies are typically coated onto magnetic beads using technical means, which then react with the sample to be tested. Simultaneously, another antigen or antibody labeled with a luminescent material also reacts with the sample, thus completing the labeling of the sample. Magnetic bead washing and separation is a crucial step, employing a strong magnetic field to separate the sample-reagent complex bound to the magnetic beads from the liquid phase, removing unbound reactants.

[0004] Chinese patent application CN202321308641.4 discloses a magnetic separation and cleaning device for a chemiluminescence immunoassay analyzer, comprising a rotary disk assembly, a liquid injection assembly, a magnetic bead adsorption assembly, and a liquid suction assembly. A reaction cup is mounted on the rotating end of the rotary disk assembly. The liquid injection and suction assemblies are both mounted on the upper surface of the rotary disk assembly, with their working ends facing the opening of the reaction cup. The magnetic bead adsorption assembly is mounted on the side of the rotary disk assembly, with its working end also facing the side of the reaction cup. This device utilizes double-sided adsorption to fully separate the magnetic beads within the reaction cup, leaving space for liquid suction. Simultaneously, it increases the adsorption force on the magnetic particles within the reaction cup, reducing magnetic loss during waste liquid suction. The three adsorption sections increase the adsorption time of the magnetic beads, and each magnetic adsorption section is connected to the adjacent section. The magnetic particles within the reaction cup are adsorbed on different sides, improving the cleaning effect and the accuracy of the analytical results. This device has an ingenious structure, excellent cleaning and separation effect, high efficiency, and good accuracy.

[0005] However, most existing magnetic separation and cleaning devices use the magnetic field generated by permanent magnets to separate the complex from the liquid phase. Since the magnetic field strength of the permanent magnets is not adjustable, when the magnetic field strength is high, the magnetic beads are strongly attracted, and impurities are easily trapped between the magnetic beads, resulting in incomplete cleaning and affecting the accuracy of the test results. When the magnets gradually demagnetize over a long period of use, the magnetic field strength weakens, the magnetic beads are less attracted, and the magnetic beads are lost during cleaning, affecting the accuracy of the test results. Utility Model Content

[0006] To address the above problems, this invention provides a chemiluminescence immunoassay analyzer and its magnetic washing structure, which has advantages such as high compatibility with different magnetic beads and accurate detection results.

[0007] The utility model discloses a support frame and setting on the support frame:

[0008] The liquid injection assembly includes a liquid injection fixing plate coaxially fixed on the support frame, and a plurality of liquid injection needles are arranged on the liquid injection fixing plate.

[0009] The liquid suction assembly includes a liquid suction fixing plate fixed above the liquid injection fixing plate, a liquid suction needle is arranged on the liquid suction fixing plate, a lead screw motor is arranged below the liquid suction fixing plate, and the lead screw motor is coaxially arranged with the liquid injection fixing plate.

[0010] The driving assembly includes a stepping motor arranged below the support frame, a transmission shaft coaxially rotates at the bottom of the support frame, the transmission shaft is connected with the stepping motor through a synchronous belt transmission structure, a rotating disc is coaxially fixed at one end of the transmission shaft away from the stepping motor, and a plurality of placement holes are arranged on the rotating disc.

[0011] The electromagnetic assembly includes an electromagnetic core below the rotating disc, the electromagnetic core is coaxially arranged with the rotating disc, a plurality of circumferentially distributed magnetic attraction blocks are fixed at the outer edge of the electromagnetic core, the number of the magnetic attraction blocks is matched with the placement holes, coils are arranged on part of the magnetic attraction blocks, and the coils are connected with a power supply device.

[0012] Preferably, the electromagnetic core and the magnetic attraction blocks are made of soft iron or silicon steel material.

[0013] Preferably, the coil is a conductive wire.

[0014] Preferably, the liquid injection needle and the liquid suction needle are spaced apart by at least two placement holes.

[0015] Compared with the prior art, the utility model has the beneficial effects that:

[0016] 1. By arranging the electromagnetic core, the magnetic attraction blocks and the coils, the compatibility of the magnetic bead cleaning for different magnetic beads is realized, the problem that the cleaning is not clean due to the large adsorption force of the magnetic beads when the magnetic field strength is high is solved, meanwhile, the magnetic attraction blocks do not demagnetize due to time growth, the magnetic field strength is not weakened, the magnetic beads are not lost during cleaning, and the accuracy of the detection result is greatly improved. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the perspective view of the utility model.

[0018] Figure 2 It is the schematic view of the rotating disc and the connecting piece in the utility model.

[0019] Figure 3It is the schematic view of the electromagnetic core in the utility model.

[0020] Figure 4 It is the schematic view of the electromagnetic core and coil in the utility model.

[0021] Figure 5 It is the schematic view of the placing hole corresponding to the process position in the utility model.

[0022] The label explanation in the schematic view is as follows:

[0023] 1, support frame; 2, liquid injection fixed plate; 3, liquid injection needle; 4, liquid suction fixed plate; 5, liquid suction needle; 6, screw rod motor; 7, transmission shaft; 8, rotating disc; 9, placing hole; 10, electromagnetic core; 11, magnetic suction block; 12, coil; 13, reaction cup inlet; 14, first liquid injection position; 15, first liquid suction position; 16, second liquid injection position; 17, second liquid suction position; 18, third liquid injection position; 19, third liquid suction position; 20, pre-excitation liquid injection position; 21, reaction cup outlet; 22, stepping motor. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0025] By Figures 1 to 5 It is given, including the liquid injection assembly arranged on the support frame 1, the liquid injection assembly includes the liquid injection fixed plate 2 coaxially fixed on the support frame 1, and a plurality of liquid injection needles 3 are arranged on the liquid injection fixed plate 2.

[0026] The liquid injection assembly includes the liquid injection fixed plate 2 coaxially fixed on the support frame 1, and a plurality of liquid injection needles 3 are arranged on the liquid injection fixed plate 2.

[0027] The liquid suction assembly includes the liquid suction fixed plate 4 fixed above the liquid injection fixed plate 2, the liquid suction needle 5 is arranged on the liquid suction fixed plate 4, the screw rod motor 6 is arranged below the liquid suction fixed plate 4, the liquid suction fixed plate 4 is in transmission connection with the screw rod motor 6, and the screw rod motor 6 is coaxially arranged with the liquid injection fixed plate 2.

[0028] The driving assembly includes the stepping motor 22 arranged below the support frame 1, the bottom of the support frame 1 is coaxially rotatable with the transmission shaft 7, the transmission shaft 7 is connected with the stepping motor 22 through the synchronous belt transmission structure, the transmission shaft 7 is coaxially fixed with the rotating disc 8 at the end away from the stepping motor 22, when the stepping motor 22 rotates, the rotating disc 8 rotates under the transmission of the synchronous belt and the transmission shaft 7, and a plurality of circumferentially distributed placing holes 9 are formed in the rotating disc 8.

[0029] Considering that the existing magnetic separation cleaning device adopts a permanent magnet group to generate a magnetic field to separate the complex from the liquid phase, since the magnetic field strength of the permanent magnet group is not adjustable, when the magnetic field strength is high, the adsorption of the magnetic beads is strong, and the magnetic beads are easy to wrap the impurities, resulting in incomplete cleaning and affecting the accuracy of the detection result; when the magnet is used for a long time, the magnetism is gradually lost, resulting in a weak magnetic field strength and a weak adsorption of the magnetic beads, causing the magnetic beads to be lost during cleaning and affecting the accuracy of the detection result, the electromagnetic assembly comprises an electromagnetic core 10 located below the rotating disc 8, the electromagnetic core 10 is coaxially arranged with the rotating disc 8, a plurality of circumferentially distributed magnetic attraction blocks 11 are fixed to the outer edge of the electromagnetic core 10, the number of the magnetic attraction blocks 11 is matched with the placement holes 9, and coils 12 are arranged on part of the magnetic attraction blocks 11.

[0030] The electromagnetic core 10 and the magnetic attraction blocks 11 are made of soft iron or silicon steel material, and immediately demagnetize after the coils 12 are powered off.

[0031] The coils 12 are conductive wires, in the embodiment, the coils 12 adopt brass wires, and the user can adjust the size of the current passing through the coils 12 to adjust the size of the magnetic field generated by the electromagnet.

[0032] Reference Figure 4 As shown in the figure, the liquid injection needle 3 and the liquid suction needle 5 are at least spaced apart by two placement holes 9.

[0033] In the embodiment, the process position of the placement hole 9 and the winding state of the coil 12 need to be further supplemented as follows:

[0034] Reference Figure 5 As shown in the figure, the placement hole 9 is sequentially divided into a reaction cup placing opening 13, a first liquid injection position 14, a first liquid suction position 15, a second liquid injection position 16, a second liquid suction position 17, a third liquid injection position 18, a third liquid suction position 19, a pre-priming liquid injection position 20 and a reaction cup grabbing opening 21, the first liquid injection position 14 and the first liquid suction position 15 are the cleaning positions of the first stage, and the second and third stages are sequentially arranged, and the liquid injection position and the liquid suction position in each stage are spaced apart by two placement holes 9, but it should be noted that the coils 12 are not wound on the magnetic attraction blocks 11 corresponding to the liquid injection positions of the second stage and the third stage, and the advantages of this arrangement are that when the reaction cup reaches the liquid injection position of the second stage and the third stage, the substances adsorbed on the magnetic beads are in a floating state again, and after the liquid injection is completed, the required substances are adsorbed on the magnetic beads again when moving to the next liquid suction position, so that the cleaning effect of the magnetic separation cleaning device is better, and the adhesion of impurities on the magnetic beads is prevented.

[0035] When the utility model is used:

[0036] Firstly, the magnetic separation and cleaning device has a three-stage cleaning process, after the coil 12 is powered on, in the first stage, the reaction cup is placed in the reaction cup placing port 13 by the grabbing mechanism, and the magnetic suction block 11 below slowly adsorbs the magnetic beads;

[0037] Then, the stepper motor 22 drives the rotating disc 8 to rotate, the reaction cup sequentially passes through the reaction cup placing port 13, the first liquid injection position 14 and the first liquid suction position 15 according to the set rhythm, and the first-stage cleaning of the magnetic beads is completed, the coil 12 is arranged on the magnetic suction block 11 on the path from the reaction cup placing port 13 to the first liquid suction position 15;

[0038] Finally, the difference between the second-stage cleaning and the third-stage cleaning and the first-stage cleaning is that: the coil 12 is not arranged on the magnetic suction block 11 below the liquid injection position, and the stepper motor 22 drives the rotating disc 8 to rotate, so that the reaction cup rotates left and right by a certain angle when the liquid is injected, so that the magnetic beads adsorbed on the side wall of the reaction cup are resuspended in the liquid when the cleaning liquid is injected.

[0039] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A chemiluminescent immunoassay device and a magnetic washing structure thereof, characterized by comprising: The support frame (1) and a liquid injection assembly arranged on the support frame (1) are provided. The liquid injection assembly comprises a liquid injection fixing plate (2) coaxially fixed on the support frame (1), and a plurality of liquid injection needles (3) arranged on the liquid injection fixing plate (2). The liquid suction assembly comprises a liquid suction fixing plate (4) fixed above the liquid injection fixing plate (2), and a liquid suction needle (5) arranged on the liquid suction fixing plate (4). The liquid suction assembly further comprises a lead screw motor (6) arranged below the liquid suction fixing plate (4), and the lead screw motor (6) is coaxially arranged with the liquid injection fixing plate (2). The driving assembly comprises a stepping motor (22) arranged below the support frame (1), and a transmission shaft (7) coaxially arranged at the bottom of the support frame (1).

2. The chemiluminescent immunoassay device and its magnetic washing structure according to claim 1, wherein, The transmission shaft (7) is connected with the stepping motor (22) through a synchronous belt transmission structure.

3. The chemiluminescent immunoassay device and its magnetic washing structure according to claim 2, wherein, The transmission shaft (7) is coaxially fixed with a rotating disc (8) at the end away from the stepping motor (22).

4. The chemiluminescent immunoassay device and its magnetic washing structure according to claim 3, wherein, The rotating disc (8) is provided with a plurality of circumferentially distributed placement holes (9). The electromagnetic assembly comprises an electromagnetic core (10) arranged below the rotating disc (8). The electromagnetic core (10) is coaxially arranged with the rotating disc (8). The electromagnetic core (10) is fixed with a plurality of circumferentially distributed magnetic attraction blocks (11) at the outer edge. The number of the magnetic attraction blocks (11) matches the number of the placement holes (9). Some of the magnetic attraction blocks (11) are provided with coils (12). The coils (12) are connected with a power supply device. The electromagnetic core (10) and the magnetic attraction blocks (11) are made of soft iron or silicon steel. The coils (12) are made of conductive wires. The liquid injection needles (3) and the liquid suction needles (5) are spaced apart by at least two placement holes (9).

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