ELISA detection kit

Abstract

The utility model discloses an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit, which belongs to the technical field of biological detection equipment and comprises a microwell plate, a box body and a top cover, the box body and the top cover are matched with each other, an inner box is movably connected in the box body, a buffer component is arranged between the box body and the inner box, and a partition plate is fixedly connected in the middle of the inner box. A supporting net for placing the micro-pore plate is fixedly connected into the inner box above the partition plate, and a vibration assembly is fixedly mounted at the bottom of the inner box. According to the ELISA detection kit, the supporting net is used for supporting the microwell plate, the top cover is matched with the pressing block and the elastic assembly to fix the microwell plate, then the vibration assembly is matched to vibrate the inner box and the microwell plate, waste liquid passes through the supporting net, and the inner box is matched with the partition plate to temporarily store the waste liquid. The problem that a worker needs to hold the kit to move back and forth among the placing unit, the vibration unit and the waste liquid cylinder, and time and labor are wasted is solved, and the detection efficiency is improved.

Description

Technical Field

[0001] This utility model belongs to the field of biological detection equipment technology, and in particular relates to an ELISA detection kit. Background Technology

[0002] ELISA kits, also known as enzyme-linked immunosorbent assay kits, are detection devices that utilize the specific binding of antibody molecules to antigen molecules to separate free contaminating proteins from the target protein bound to a solid-phase carrier. Specific labels are then used for qualitative or quantitative analysis. ELISA is a highly sensitive, specific, and reproducible diagnostic method. Due to its stable reagents, ease of storage, simple operation, and objective results, it has been widely used in various fields of immunological testing.

[0003] Existing ELISA test kits often require the use of vibration devices to accelerate the mixing of internal drugs during testing. However, traditional vibration devices mostly require operators to hold the kit and place it against the vibration unit, which is quite limiting. After the test, washing is also required to remove any substances that could not bind to the solid-phase antigen or antibody in the wells. Operators need to hold the kit and move back and forth between the placement unit, the vibration unit, and the waste container, which is time-consuming, labor-intensive, and affects the testing efficiency.

[0004] To address these issues, we propose an ELISA detection kit. Utility Model Content

[0005] The purpose of this invention is to solve the problems of time-consuming and labor-intensive detection steps and low detection efficiency in the existing technology, and to propose an ELISA detection kit.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An ELISA test kit includes a microplate, a box body and a top cover that cooperate with each other, an inner box that is movably connected inside the box body, and a buffer assembly between the box body and the inner box. A partition plate is fixedly connected to the middle of the inner box, and a support mesh for placing the microplate is fixedly connected inside the inner box above the partition plate. A vibration assembly is fixedly installed at the bottom of the inner box.

[0008] Elastic bands are symmetrically arranged on both sides of the box body, and the top cover is locked and sealed by the elastic bands. A pressure block is provided inside the top cover, which moves up and down. An elastic component is provided between the pressure block and the top cover, and the pressure block is driven downward to press and fix the microporous plate by the elastic component.

[0009] Preferably, a plurality of sliding rods are fixedly connected to the bottom wall of the inner box, and a plurality of sliding holes for the sliding rods to be inserted are opened on the bottom surface of the inner box. The buffer assembly includes a plurality of buffer blocks fixedly connected to the bottom wall and the inner side wall of the inner box, and the other side of the buffer block is attached to the outer wall of the inner box.

[0010] Preferably, rotating blocks are rotatably connected to both ends of the box body, and the elastic band is fixedly connected between the two rotating blocks on the same side.

[0011] Preferably, the top surface of the top cover has a first groove on both sides for placing the elastic band.

[0012] Preferably, the top surface of the top cover has a second groove perpendicular to the first groove, and the depth of the second groove is greater than that of the first groove.

[0013] Preferably, the bottom surface of the top cover is fixedly connected to a guide rail, the pressure block is slidably connected to the guide rail, and the elastic component includes a spring disposed on the upper part of the guide rail.

[0014] Preferably, when the pressure block is at the top of the stroke, the bottom surface of the pressure block is lower than the bottom of the guide rail.

[0015] In summary, the technical effects and advantages of this utility model are as follows: This ELISA test kit supports the microplate through a support mesh, and fixes the microplate through a top cover, pressure block, and elastic component. Furthermore, a vibration component vibrates the inner box and the microplate. The support mesh allows waste liquid to pass through, and the inner box, with a separator plate, temporarily stores the waste liquid. Compared to existing devices, this avoids the time-consuming and labor-intensive problem of staff having to carry the test kit back and forth between the placement unit, vibration unit, and waste liquid tank, thus improving testing efficiency.

[0016] In addition, the high-frequency vibration of the inner box is filtered out by the buffer block, and the top cover is fixed by the elastic band in conjunction with the first groove. Compared with the existing device, the high-frequency vibration is avoided from damaging the top cover and the box body, and the service life of the device is improved. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a cross-sectional front view of the present invention;

[0019] Figure 3 This is a top cross-sectional view of the present invention;

[0020] Figure 4 for Figure 2 Enlarged view of point A in the middle.

[0021] In the diagram: 1. Microporous plate; 2. Box body; 3. Top cover; 4. Inner box; 5. Divider plate; 6. Support mesh; 7. Vibration assembly; 8. Elastic band; 9. Pressure block; 10. Sliding rod; 11. Buffer block; 12. Rotating block; 13. First groove; 14. Second groove; 15. Guide rail; 16. Spring; 17. Water outlet pipe. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0023] Reference Figure 1-3 An ELISA test kit includes a microplate 1, a box body 2 and a top cover 3 that cooperate with each other. An inner box 4 is movably connected inside the box body 2, and a buffer component is provided between the box body 2 and the inner box 4. A partition plate 5 is fixedly connected to the middle of the inner box 4. A support net 6 for placing the microplate 1 is fixedly connected inside the inner box 4 above the partition plate 5. A vibration component 7 is fixedly installed at the bottom of the inner box 4.

[0024] Elastic bands 8 are symmetrically arranged on both sides of the box body 2, and the top cover 3 is locked and sealed by the elastic bands 8. The top cover 3 has a pressing block 9 that moves up and down inside. An elastic component is provided between the pressing block 9 and the top cover 3, and the pressing block 9 is driven to press down and fix the microporous plate 1 by the elastic component.

[0025] Reference Figure 1-3 When using this ELISA test kit, first place the microplate 1 on the support net 6, then place and press down the top cover 3. The pressure block 9 on the top cover 3 is pressed and fixed by the elastic component. Continue to press down the top cover 3 until the top cover 3 fits against the box body 2. At this time, the top cover 3 is locked by the elastic band 8.

[0026] Once completed, the vibration component 7 is activated, which drives the inner box 4 to vibrate. The microporous plate 1 on the support mesh 6 vibrates accordingly, allowing the drug in the microporous plate 1 to be mixed. After mixing, the drug can be tested.

[0027] After the test is completed, pour the waste liquid from microplate 1 into inner box 4, then add washing liquid to microplate 1. After waiting for a period of time, pour the washing waste liquid into inner box 4. During the waiting period, the above vibration steps can be repeated to vibrate microplate 1, thereby improving the washing effect and efficiency.

[0028] It should be noted that a water outlet pipe 17 located above the partition plate 5 should be fixedly connected to one side of the inner box 4. A through hole for the water outlet pipe 17 to extend from the side wall of the box body 2 is provided. A water baffle can be detachably installed at the end of the water outlet pipe 17. Since the water outlet pipe 17 and the water baffle are existing technologies, they will not be described in detail. After washing, open the water baffle and drain all the waste liquid through the water outlet pipe 17. The above is the complete usage process of this ELISA test kit.

[0029] Reference Figure 1-3 Multiple sliding rods 10 are fixedly connected to the bottom wall of the inner box 2. Multiple sliding holes for inserting the sliding rods 10 are provided on the bottom surface of the inner box 4. The buffer assembly includes multiple buffer blocks 11 fixedly connected to the bottom wall and inner side wall of the inner box 2, with the other side of each buffer block 11 fitting against the outer wall of the inner box 4. The sliding rods 10, in conjunction with the sliding holes, limit the movement of the inner box 4, while the buffer blocks 11 cushion the vibration of the inner box 4, improving the stability and service life of the device.

[0030] Rotating blocks 12 are rotatably connected to both ends of the box body 2. The elastic band 8 is fixedly connected between the two rotating blocks 12 on the same side. The rotation of the elastic band 8 is achieved by rotating blocks 12, which facilitates the locking and unlocking of the top cover 3 by the elastic band 8.

[0031] To prevent the elastic band 8 from sliding during locking, which could cause the top cover 3 to become unstable, the top surface of the top cover 3 has a first groove 13 on both sides for the elastic band 8 to be placed. The first groove 13 limits the elastic band 8 and improves the stability of the device.

[0032] The top surface of the top cover 3 has a second groove 14 that is perpendicular to the first groove 13, and the depth of the second groove 14 is greater than that of the first groove 13. The design of the second groove 14 makes it easier for workers to pick up the elastic band 8 and improves work efficiency.

[0033] Reference Figure 2 , Figure 4 A guide rail 15 is fixedly connected to the bottom surface of the top cover 3. A pressure block 9 is slidably connected within the guide rail 15. The elastic component includes a spring 16 located on the upper part of the guide rail 15. The spring 16, in conjunction with the guide rail 15, presses down the pressure block 9, thereby fixing the microporous plate 1. Two pressure blocks 9 can be symmetrically arranged above the edge of the inner box 4 to ensure uniform force on the microporous plate 1 and minimize contact with the holes in the microporous plate 1. When the pressure block 9 is at the top of its stroke, its bottom surface is lower than the bottom of the guide rail 15, thus preventing the guide rail 15 from directly contacting the microporous plate 1.

[0034] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An ELISA detection kit, comprising a microplate (1), a housing (2) and a top cover (3) that cooperate with each other, characterized in that, The inner box (4) is movably connected inside the box body (2), and a buffer assembly is provided between the box body (2) and the inner box (4). A partition plate (5) is fixedly connected in the middle of the inner box (4). A support net (6) for placing the microporous plate (1) is fixedly connected inside the inner box (4) above the partition plate (5). A vibration assembly (7) is fixedly installed at the bottom of the inner box (4). The box body (2) is symmetrically provided with elastic bands (8) on both sides, and the top cover (3) is locked and sealed by the elastic bands (8). The top cover (3) is provided with a pressure block (9) that moves up and down inside. An elastic component is provided between the pressure block (9) and the top cover (3), and the pressure block (9) is driven by the elastic component to press down and fix the microporous plate (1).

2. The ELISA detection kit according to claim 1, characterized in that, The inner bottom wall of the box (2) is fixedly connected to a plurality of sliding rods (10), and the bottom surface of the inner box (4) is provided with a plurality of sliding holes for the sliding rods (10) to be inserted. The buffer assembly includes a plurality of buffer blocks (11) fixedly connected to the inner bottom wall and the inner side wall of the box (2), and the other side of the buffer block (11) is attached to the outer wall of the inner box (4).

3. The ELISA detection kit according to claim 1, characterized in that, The box body (2) has rotating blocks (12) rotatably connected to both ends, and the elastic band (8) is fixedly connected between the two rotating blocks (12) on the same side.

4. The ELISA detection kit according to claim 3, characterized in that, The top cover (3) has a first groove (13) on both sides of the top surface for placing the elastic band (8).

5. The ELISA detection kit according to claim 4, characterized in that, The top surface of the top cover (3) is provided with a second groove (14) perpendicular to the first groove (13), and the depth of the second groove (14) is greater than that of the first groove (13).

6. The ELISA detection kit according to claim 1, characterized in that, The bottom surface of the top cover (3) is fixedly connected to a guide rail (15), and the pressure block (9) is slidably connected to the guide rail (15) up and down. The elastic component includes a spring (16) disposed on the upper part of the guide rail (15).

7. The ELISA detection kit according to claim 6, characterized in that, When the pressure block (9) is at the top of the stroke, the bottom surface of the pressure block (9) is lower than the bottom of the guide rail (15).

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