Cell culture patch

Abstract

The utility model relates to a cell culture patch which comprises a cell culture plate, a patch body is adhered to the upper surface of the cell culture plate, the thickness of the patch body is 0.5-3.0 mm, a patterned hollow structure is formed in the center of the patch body, and the thickness of the patch body is 0.5-3.0 mm. The patch body is provided with a plurality of groups of independent and mutually matched glue injection inlets and outlets, and the plurality of glue injection inlets and outlets are communicated with the patterned hollow structure. According to the utility model, spatial arrangement of various cells and extracellular matrixes is realized in a simple manner, so that the constructed 3D cell structure has a bionic function, and meanwhile, the extracellular matrixes with uniform height can uniformly diffuse nutrients of a culture medium into the extracellular matrixes.

Description

Technical Field

[0001] This utility model relates to a cell culture patch, and more particularly to a patterned 3D cell culture patch. Background Technology

[0002] With the development of cell biology and organoid technology, three-dimensional cell culture technology is gradually replacing traditional two-dimensional cell culture technology. Currently, many cell types possess strong self-assembly capabilities, such as pluripotent stem cells, tumor cells, and tissue cells. Three-dimensional cell spheres are three-dimensional aggregates formed by the self-assembly of various cells, more closely resembling the structural morphology of in vivo tissue cells and more conducive to the study of their functional mechanisms. Therefore, three-dimensional cell spheres can be used in numerous biological and biomedical research fields, such as developmental biology, pathology, pharmacology, and cancer treatment.

[0003] A search revealed the patent: "A Device and Method for Cell Patterning Culture Based on 3D Printing Technology" (CN201710118294.1). This invention relates to a device and method for rapidly preparing patterned cell co-culture based on 3D printing. This application provides a technology for rapidly preparing microdevices for cell culture and patterned cell co-culture using 3D printing, and this device is easily compatible with existing cell biotechnology techniques, such as microscopic imaging and cell immunostaining. It can easily achieve patterning of various cell types and perform microscopic imaging and immunohistochemical analysis of cells to obtain quantitative results. The aforementioned structural operations are inconvenient and inefficient.

[0004] In view of the above-mentioned shortcomings, the designer has actively researched and innovated in order to create a new type of cell culture patch with greater industrial application value. Utility Model Content

[0005] To address the aforementioned technical problems, the purpose of this invention is to provide a cell culture patch.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A cell culture patch includes a cell culture plate, on the upper surface of which a patch body is adhered. The thickness of the patch body is between 0.5 mm and 3.0 mm. A patterned perforated structure is formed in the center of the patch body. Several sets of independent and mutually cooperating glue injection ports are formed on the patch body, and several glue injection ports are connected to the patterned perforated structure.

[0008] Preferably, in the cell culture patch, the cell culture plate is a 6-, 12-, or 24-well cell culture plate.

[0009] Preferably, the cell culture patch, the patch body adopts a viscoelastic material.

[0010] Preferably, the cell culture patch, the patch body is prepared by mold injection or is provided by hot stamping.

[0011] Preferably, the cell culture patch, the patterned hollow structure is a square structure, and a group of glue injection outlets are provided and communicate with the patterned hollow structure of the square structure.

[0012] Preferably, the cell culture patch, the patterned hollow structure is three parallel rectangular structures, and the three rectangular structures are separated by a single-row rectangular fence structure; and three groups of glue injection outlets are provided on the patch body, and each group of glue injection outlets corresponds to and communicates with the patterned hollow structure of each rectangular structure.

[0013] Preferably, the cell culture patch, the patterned hollow structure is three groups of circular structures connected in series, and one group of glue injection outlets is provided on the patch body and communicates with the three groups of circular structures connected in series.

[0014] Preferably, the cell culture patch, the thickness of the patch body is 1-1.5mm.

[0015] By the above scheme, the cell culture patch has at least the following advantages:

[0016] The cell culture patch can solve the problem that the traditional glue injection method cannot realize uniform height and patterned glue injection, and can realize spatial arrangement of various cells and extracellular matrix by a simple method, so that the constructed 3D cell structure has more bionic functions, and the uniform height of the extracellular matrix can be improved, so that the nutrients of the culture medium can be uniformly diffused into the inside.

[0017] The above description is only a summary of the technical scheme of the cell culture patch, in order to more clearly understand the technical means of the cell culture patch, and the content of the specification can be implemented, the preferred embodiments of the cell culture patch will be described in detail below with reference to the drawings. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical scheme of the embodiments of the cell culture patch, the drawings needed in the embodiments will be briefly introduced, and it should be understood that the following drawings only show some embodiments of the cell culture patch, and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0019] Figure 1This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the structure of the patch body of this utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the patch body detaching from the cell culture plate according to this utility model. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0023] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0024] Example

[0025] like Figure 1 , Figure 2 and Figure 3 As shown, a cell culture patch includes a cell culture plate 40, on the upper surface of which a patch body 10 is adhered. The thickness of the patch body 10 is between 0.5 mm and 3.0 mm. A patterned perforated structure 20 is formed in the center of the patch body 10. Several sets of independent and mutually cooperating glue injection inlets and outlets 30 are formed on the patch body 10, and the glue injection inlets and outlets 30 are connected to the patterned perforated structure 20.

[0026] The cell culture plate 40 described in this invention is a cell culture plate with 6, 12, or 24 wells.

[0027] The patch body 10 described in this invention is made of a viscoelastic material, such as polydimethylsiloxane (PDMS) or food-grade silicone.

[0028] The patch body 10 described in this utility model is prepared by injection molding or by hot stamping.

[0029] The patterned hollow structure 20 described in this utility model is a square structure and is provided with a set of glue injection inlets and outlets 30, which are connected to the patterned hollow structure 20 of the square structure.

[0030] The patterned hollow structure 20 described in this utility model consists of three parallel rectangular structures, and the three rectangular structures are separated by a single row of rectangular fence structures; at the same time, three sets of glue injection inlets and outlets 30 are provided on the patch body, and each set of glue injection inlets and outlets corresponds one-to-one with each patterned hollow structure 20 of the rectangular structure and is connected to it.

[0031] The patterned hollow structure 20 described in this utility model consists of three sets of connected circular structures. The patch body is provided with one set of glue injection inlet / outlet 30, which is connected to the three sets of connected circular structures.

[0032] The thickness of the patch body 10 described in this utility model is between 1 mm and 1.5 mm.

[0033] The working principle of this utility model is as follows:

[0034] (1) The patch body is sterilized and adhered to the surface of the cell culture plate 40.

[0035] (2) Prepare the required Matrigel solution and iPSC cell suspension mixture;

[0036] (3) Place the sterilized patch in a cell culture dish or culture plate, press it gently, and place it on an ice bath;

[0037] (4) The prepared Matrigel solution and cell suspension mixture is injected into the patch through the injection port until it reaches the flow channel outlet;

[0038] (5) Place the culture plate in a 37°C incubator and let it stand for 30 minutes to allow the Matrigel to solidify.

[0039] (6) After curing, gently remove the patch to leave a patterned Matrigel structure of iPSCs on the surface of the cell culture plate. Add culture medium and carry out subsequent culture.

[0040] Example 1

[0041] Based on the embodiments, the patch body of this utility model is made of PDMS with a thickness of 1mm. The patch is compatible with existing 6-well cell culture plates. At the same time, the patch body has a patterned hollow structure 20 with a square structure and a set of gel injection inlet and outlet 30. At this time, the extracellular matrix is ​​Matrigel.

[0042] Example 2

[0043] Based on the embodiments, the patch body of this utility model is made of PDMS with a thickness of 1.5mm. The patch is compatible with existing 6-well cell culture plates. At the same time, the patch body has 3 parallel rectangular structures, and the three structures are separated by a single row of rectangular fence structures; it has 3 sets of gel injection inlets and outlets 30; at this time, the extracellular matrix is ​​Matrigel.

[0044] Example 3

[0045] Based on the embodiments, the patch body of this utility model is made of food-grade silicone with a thickness of 1.5mm. The patch is compatible with existing 6-well cell culture plates. At the same time, the patch body has 3 circular structures connected in series and a set of gel injection inlet / outlet 30. At this time, the extracellular matrix is ​​GelMA.

[0046] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0047] In the description of this application, it should be noted that the terms "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0048] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or vertical, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0049] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0050] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A cell culture patch, comprising a cell culture plate (40), characterized in that: The cell culture plate (40) has a patch body (10) attached to its upper surface. The thickness of the patch body (10) is between 0.5 mm and 3.0 mm. A patterned hollow structure (20) is provided in the center of the patch body (10). Several independent and mutually cooperating glue injection inlets and outlets (30) are provided on the patch body (10). Several glue injection inlets and outlets (30) are connected to the patterned hollow structure (20).

2. The cell culture patch according to claim 1, characterized in that: The cell culture plate (40) is a 6-well, 12-well, or 24-well cell culture plate.

3. The cell culture patch according to claim 1, characterized in that: The patch body (10) is made of viscoelastic material.

4. A cell culture patch according to claim 1, characterized in that: The patch body (10) is prepared by injection molding or by hot stamping.

5. A cell culture patch according to claim 1, characterized in that: The patterned hollow structure (20) is a square structure and is provided with a set of glue injection inlets and outlets (30), which are connected to the patterned hollow structure (20) of the square structure.

6. A cell culture patch according to claim 1, characterized in that: The patterned hollow structure (20) consists of three parallel rectangular structures, and the three rectangular structures are separated by a single row of rectangular fence structures; at the same time, three sets of glue injection inlets and outlets (30) are provided on the patch body, and each set of glue injection inlets and outlets corresponds one-to-one with each patterned hollow structure (20) of the rectangular structure and is connected.

7. A cell culture patch according to claim 1, characterized in that: The patterned hollow structure (20) consists of three sets of connected circular structures. The patch body is provided with one set of glue injection inlet and outlet (30), which is connected to the three sets of connected circular structures.

8. A cell culture patch according to claim 1, characterized in that: The thickness of the patch body (10) is between 1 mm and 1.5 mm.

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