Pipeline type automatic sampling system
By using a tubular automated sample introduction system, combined with a peristaltic pump and a vibration motor, automatic sampling, staining, sample introduction, and cleaning are achieved. This solves the shortcomings of automated sample introduction technology in fluorescence analyzers, improves the accuracy and safety of detection, and adapts to complex experimental needs.
Patent Information
- Application Number
- CN202422489129.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-15
- Publication Date
- 2026-02-03
- Estimated Expiration
- 2034-10-15
AI Technical Summary
The development of automated sample introduction technology in somatic cell detection using existing fluorescence analysis instruments is insufficient. Manual sample introduction suffers from low accuracy, poor repeatability, low efficiency, and safety hazards. Existing automated sample introduction systems also suffer from problems such as aging, cross-contamination, and insufficient cleaning.
The system employs a tubular automated sampling system, including various peristaltic pumps and vibration motors, combined with microcontroller and host computer control, to achieve automated sampling, staining, injection, and cleaning. It uses six-roller and four-roller peristaltic pump heads to ensure high accuracy and repeatability. Fluid transfer is controlled by electromagnets, achieving multi-module integration and high automation.
It improves the accuracy and repeatability of the sample introduction system, reduces human error, increases processing speed and analysis efficiency, reduces the risk of cross-contamination, ensures operational safety, and adapts to complex experimental procedures and multiple sample requirements.
Smart Images
Figure CN223870513U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to an automatic sampling system, concretely relates to a pipeline type automatic sampling system matched with an online milk somatic cell fluorescence analysis system. BACKGROUND
[0002] Fluorescence analysis is a qualitative and quantitative analysis technique based on molecular fluorescence phenomenon. Its principle lies in the use of fluorescent dyes to label specific components in cells. When these fluorescent molecules absorb excitation light of a specific wavelength, they will transition to a high-energy state and then quickly return to the ground state, emitting longer-wavelength fluorescence. After processing by filters and optical systems, these emitted lights are amplified and recorded, ultimately forming a fluorescence image of the sample. Observing these images with a fluorescence microscope allows analysis of the distribution and dynamic changes of the markers in the sample. Fluorescence analysis is widely used in cell analysis, for example, when using fluorescence analysis instruments for somatic cell detection, a small amount of sample is usually manually injected into a specific detection pool or chip using a syringe or a pipette, and then automatically analyzed by the instrument.
[0003] In the field of fluorescence analysis, compared with the traditional manual syringe or pipette sampling method in the laboratory, the automatic sampling system ensures the accuracy and consistency of each sampling through mechanization and standardization, significantly reduces human error, and improves the accuracy and repeatability of the results. The automatic sampling system can automatically process multiple samples without human intervention, greatly improving the sample processing speed and experimental efficiency, especially in high-throughput analysis. In addition, the closed system reduces the risk of sample exposure to the external environment, reduces the possibility of contamination, and improves the safety of handling hazardous or corrosive samples. The automatic sampling system is not only suitable for high-sensitivity analysis and micro-sample processing, but also can be flexibly programmed according to complex experimental procedures and diverse sample requirements, becoming an indispensable tool in modern fluorescence analysis.
[0004] The rapid development of automatic sampling systems is due to the progress of precision machining technology, micro-motors and transmission devices, the application of various high-precision miniaturized intelligent sensors, modern control theory and automation technology, as well as the application of microfluidic technology and new materials. The comprehensive application and continuous progress of these technologies have promoted the development of automatic sampling devices, making them play an increasingly important role in various types of analysis and detection and laboratory applications.
[0005] In the field of fluorescence analysis, specifically in the field of somatic cell detection and counting, although the technology is developing rapidly at present, the mainstream way of sample injection is still manual sample injection, especially for some instruments used for teaching and scientific research, there are few automatic sample injection and online detection functions. The automatic level of fluorescence analysis instruments on the market is low, and during the analysis and testing process, multiple sampling and sample injection are usually required, which has the problems of low accuracy, poor repeatability and stability, low efficiency and the like. When dealing with rapid detection events, most of the processes are carried out manually, and there is a great safety hazard when using some toxic reagents.
[0006] In contrast, a more ideal sample injection method is automatic sample injection, and various automatic sample injection systems have appeared on the market, such as automatic pipetting sample injection systems, pipeline transfer sample injection systems, and flow cytometry automatic sample injection systems. Among these sample injection systems, the technology of using a six-way valve combined with a water pump is relatively traditional, which has the problems of easy aging of ordinary water pumps, lack of automatic shaking steps, need for manual assistance for sample shaking, easy cross contamination of samples, and insufficient cleaning due to single direction of liquid delivery. There is another relatively precise sample injection method, that is, the automatic pipetting sample injection technology using a sample injection disc combined with a mechanical arm and a sample injection needle, but this technology has high integration difficulty, complex control, high system failure rate, and large occupied space. The design of this sample injection carousel structure is widely used in automatic sample injection devices, but often lacks real-time automatic cleaning process for the matching pipeline and liquid needle.
[0007] Manual sample injection is easily affected by the technical level of the operator, hand shaking and environmental changes, resulting in inaccurate and inconsistent sample injection volume, which affects the accuracy and repeatability of the experimental results. The manual sample injection process is tedious, especially when a large number of samples are analyzed, which is time-consuming and inefficient. In addition, samples are easily contaminated, especially when handling trace or high sensitivity samples, the situation is particularly serious. When handling toxic, harmful or corrosive samples, manual sample injection also increases the exposure risk of the operator. At present, the field of fluorescence analysis still mainly uses manual sample injection, and lacks a simple control, high accuracy and perfect process automatic sample injection technology.
[0008] Therefore, it is a technical problem to be solved to provide an automatic sample injection system which is widely applicable, high in stability, perfect in process, easy to integrate and high in automation. Practical new type content
[0009] In order to solve the problems and needs in the background art, the present application provides a pipeline type automatic sample injection system.
[0010] The technical scheme adopted by the present application is:
[0011] The pipeline type automatic sampling system comprises a second cleaning pump, a sample pump, a feeding pump, a dye pump, a second waste liquid pump, a clean water bottle, a sample bottle, a dye bottle, a waste liquid bottle, a sample mixing pool and a control module; the input port of the second cleaning pump is communicated with the clean water bottle, and the output port of the second cleaning pump is communicated with the inlet of the sample mixing pool; the input port of the sample pump is communicated with the sample bottle, and the output port of the sample pump is communicated with the inlet of the sample mixing pool; the outlet of the sample mixing pool is connected with an on-off valve through a check valve, the input port of the feeding pump is communicated with the outlet of the sample mixing pool through the on-off valve, and the output port of the feeding pump is connected with a detection area; the input port of the dye pump is communicated with the dye bottle, and the output port of the dye pump is communicated with the inlet of the sample mixing pool; the input port of the second waste liquid pump is communicated with the outlet of the sample mixing pool through the on-off valve, and the output port of the second waste liquid pump is communicated with the waste liquid bottle; and the second cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump are connected with the control module.
[0012] The pipeline type automatic sampling system comprises a second cleaning pump, a sample pump, a feeding pump, a dye pump, a second waste liquid pump, a clean water bottle, a sample bottle, a dye bottle, a waste liquid bottle, a sample mixing pool and a control module; the input port of the second cleaning pump is communicated with the clean water bottle, and the output port of the second cleaning pump is communicated with the inlet of the sample mixing pool; the input port of the sample pump is communicated with the sample bottle, and the output port of the sample pump is communicated with the inlet of the sample mixing pool; the outlet of the sample mixing pool is connected with an on-off valve through a check valve, the input port of the feeding pump is communicated with the outlet of the sample mixing pool through the on-off valve, and the output port of the feeding pump is connected with a detection area; the input port of the dye pump is communicated with the dye bottle, and the output port of the dye pump is communicated with the inlet of the sample mixing pool; the input port of the second waste liquid pump is communicated with the outlet of the sample mixing pool through the on-off valve, and the output port of the second waste liquid pump is communicated with the waste liquid bottle; and the second cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump are connected with the control module.
[0013] The on-off valve comprises a two-position three-way valve.
[0014] The detection area is an observation chamber in a micro-fluidic chip.
[0015] The first cleaning pump, the second cleaning pump, the sample pump, the feeding pump, the dye pump, the first waste liquid pump and the second waste liquid pump comprise one of a six-roller peristaltic pump head and a four-roller peristaltic pump head.
[0016] A vibration motor is further arranged near a pipeline of the sample mixing pool, and is used for shaking liquid at an input pipeline of the sample mixing pool into the sample mixing pool and automatically vibrating a body of the sample mixing pool.
[0017] Corresponding stroke electromagnet control modules are arranged at the second cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump, and all stroke electromagnets are electrically connected with the stroke electromagnet control modules, so that the second cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump are controlled.
[0018] The sample bottle comprises a milk pool.
[0019] The number of the sample bottles is multiple, and the multiple sample bottles are communicated with the input port of the sample pump through a multi-way pipeline.
[0020] The control module comprises a microcontroller and an upper computer, and the second cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump are connected with the upper computer through the microcontroller.
[0021] The pipeline type automatic sampling system comprises a second cleaning pump, a sample pump, a feeding pump, a dye pump, a second waste liquid pump, a clean water bottle, a sample bottle, a dye bottle, a waste liquid bottle, a sample mixing pool and a control module; the input port of the second cleaning pump is communicated with the clean water bottle, and the output port of the second cleaning pump is communicated with the inlet of the sample mixing pool; the input port of the sample pump is communicated with the sample bottle, and the output port of the sample pump is communicated with the inlet of the sample mixing pool; the outlet of the sample mixing pool is connected with an on-off valve through a check valve, the input port of the feeding pump is communicated with the outlet of the sample mixing pool through the on-off valve, and the output port of the feeding pump is connected with a detection area; the input port of the dye pump is communicated with the dye bottle, and the output port of the dye pump is communicated with the inlet of the sample mixing pool; the input port of the second waste liquid pump is communicated with the outlet of the sample mixing pool through the on-off valve, and the output port of the second waste liquid pump is communicated with the waste liquid bottle; and the second cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump are connected with the control module.
[0022] The utility model provides a pipeline formula automatic sampling system overcomes manual sampling and prior art low degree of automation, stability accuracy is insufficient's defect, has high accuracy and repeatability, multi -module integration, has improved processing speed and analysis efficiency significantly.
[0023] The utility model discloses a pipeline formula automatic sampling system has high accuracy and repeatability, uses microcontroller to combine the automatic control of host computer, improved processing speed and analysis efficiency significantly. BRIEF DESCRIPTION OF DRAWINGS
[0024] Figure 1 It is the front view of pipeline formula automatic sampling system that the utility model provides.
[0025] Figure 2 It is the pipeline schematic drawing of system sampling operation.
[0026] Figure 3 It is the pipeline schematic drawing of system waste liquid operation.
[0027] Figure 4 It is the pipeline schematic drawing of system cleaning operation.
[0028] Figure 5 It is the back view of pipeline formula sampling system.
[0029] Figure 6 It is the structure schematic drawing of stroke electromagnet fixed frame.
[0030] In the drawing: first cleaning pump 1, second cleaning pump 2, sample pump 3, feeding pump 4, dye pump 5, first waste liquid pump 6, second waste liquid pump 7, clean water bottle 8, sample bottle 9, dye bottle 10, waste liquid bottle 11, sample mixing pool 12, check valve 15. DETAILED DESCRIPTION
[0031] The utility model will be further explained in detail in connection with the drawings and specific embodiment.
[0032] The utility model discloses a pipeline formula automatic sampling system has high accuracy and repeatability, uses microcontroller to combine the automatic control of host computer, improved processing speed and analysis efficiency significantly.
[0033] As Figure 1The utility model provides a pipeline type automatic sample introduction system, which comprises a first cleaning pump 1, a first waste liquid pump 6, a second cleaning pump 2, a sample pump 3, a feeding pump 4, a dye pump 5, a second waste liquid pump 7, a clean water bottle 8, a sample bottle 9, a dye bottle 10, a waste liquid bottle 11, a mixed sample pool 12 and a control module. The input port of the first cleaning pump 1 is communicated with the clean water bottle 8, and the output port of the first cleaning pump 1 is communicated with the sample bottle 9. The input port of the first waste liquid pump 6 is communicated with the sample bottle 9, and the output port of the first waste liquid pump 6 is communicated with the waste liquid bottle 11. The input port of the second cleaning pump 2 is communicated with the clean water bottle 8, and the output port of the second cleaning pump 2 is communicated with the inlet of the mixed sample pool 12. The input port of the sample pump 3 is communicated with the sample bottle 9, and the output port of the sample pump 3 is communicated with the inlet of the mixed sample pool 12. The outlet of the mixed sample pool 12 is connected with a switch valve through a check valve 15, the input port of the feeding pump 4 is communicated with the outlet of the mixed sample pool 12 through the switch valve, and the output port of the feeding pump 4 is connected with a detection area. The input port of the dye pump 5 is communicated with the dye bottle 10, and the output port of the dye pump 5 is communicated with the inlet of the mixed sample pool 12. The input port of the second waste liquid pump 7 is communicated with the outlet of the mixed sample pool 12 through the switch valve, and the output port of the second waste liquid pump 7 is communicated with the waste liquid bottle 11. The second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5 and the second waste liquid pump 7 are connected with the control module.
[0034] The first cleaning pump 1 and the second cleaning pump 2 are connected with the clean water bottle 8 at one end, used for pumping clean water, the pump is relatively large, so the precision is relatively lower than that of the sample pump 3, the feeding pump 4 and the dye pump 5, and the inner tube size of the pump is selected as 3*5mm inner diameter*outer diameter. The sample pump 3, the feeding pump 4 and the dye pump 5 are connected with the mixed pool 12, and have certain requirements on precision, the pump port is matched with a check valve, and the silica gel tube in the pump is preferably 0.4*3mm inner diameter*outer diameter, and the flow rate range is 0.0002-3ml / min. The peristaltic pump is driven by a stepping motor, the stepping motor can provide consistent performance for the system, ensure that the operation result is the same each time, and accurately control the position by adjusting the input pulse signal, so that accurate metering and quantitative delivery with adjustable quantity are realized. The pipeline of one end of the first waste liquid pump 6 and the second waste liquid pump 7 is connected to the waste liquid bottle 11, mainly used for pumping the waste liquid after cleaning, and the flow is not required to be accurately controlled, so a direct current motor is used for driving.
[0035] The water bottle 8, sample bottle 9, dye bottle 10 and waste liquid bottle 11 are designed with bottle caps matched with different size silicone hoses in actual implementation, which are fixed with the hoses strictly when the pump is working, facilitating use. The pipelines of the sample pump 3 and the dye pump 5 are connected to the sample bottle 9 and the dye bottle 10 at one end respectively, and the liquid in the bottles is extracted to the mixing pool 12 by driving the peristaltic pump through specific pulse signals generated by the microcontroller. It should be noted that the mixing pool 12 is provided with a vibration motor on both sides, and the rotor inside the motor can rotate at a speed of 22000 rpm. Due to the existence of the eccentric block on the rotor, the centrifugal force generated during high-speed rotation can make the whole motor vibrate, which can well mix the quantitative sample and dye system in the mixing pool, so as to ensure the dyeing effect.
[0036] The switch valve is a two-position three-way valve. The mixing pool 12 is connected to the common end of a two-position three-way electromagnetic valve 18 through a check valve 15 below the mixing pool 12. The check valve prevents backflow of the liquid, has a small volume and is easy to disassemble. One end of the vibration motor is connected to an electromagnetic relay module 21, and the microcontroller sends high and low level signals to the control port to realize on-off control of the loop. The two-position three-way electromagnetic valve 18 has three ports, namely the common end, the normally open end and the normally closed end. Here, the normally closed end is connected to the second waste liquid pump 7 through the pipeline, which is used to pump away the waste liquid later. The normally open end is connected to the peristaltic feeding pump 4, which is used to pump the sample after dyeing. The IO port of the microcontroller is connected to the control pin of the electromagnetic three-way valve, and the on-off of the electromagnetic coil in the electromagnetic valve is controlled by outputting high and low level signals, so as to control the action of the valve core, realize the on-off and regulation of the fluid, and adjust the on-off of the port in different stages of the sample injection process, and correspondingly drive different pumps to extract the liquid in the mixing pool. The use of the electromagnetic three-way valve can well seal the fluid from the actuator, realizing precise and reliable fluid transmission.
[0037] The sample bottle 9 includes a milk pool. The detection area includes an observation chamber in the microfluidic chip, and the sample is placed in the flow channel of the microfluidic chip.
[0038] As shown in Figure 5 and Figure 6 , the second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5 and the second waste liquid pump 7 are all provided with corresponding travel electromagnets, which are installed on the system mounting seat through the electromagnetic iron fixing frame. The control module is electrically connected with all the travel electromagnets, realizing the control of the second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5 and the second waste liquid pump 7. The control module includes a microcontroller and an upper computer, and the second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5 and the second waste liquid pump 7 are connected with the upper computer through the microcontroller.
[0039] The first cleaning pump 1, the second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5, the first waste liquid pump 6 and the second waste liquid pump 7 comprise one of a six-roller peristaltic pump head and a four-roller peristaltic pump head. The peristaltic pump effectively avoids cross contamination by pumping the liquid to make the liquid only contact the pump tube without contacting other parts of the pump. The pump tube is easy to clean and replace, the maintenance is simple, and bidirectional pumping can be performed. The peristaltic pump has small shear force on the liquid, is suitable for transporting biological samples and cell suspensions, and will not damage the structure. Compared with the three-roller pump head, the six-roller pump head increases the number of rollers, can reduce the pulsation and fluctuation of the fluid, is very suitable for applications requiring stable flow. More rollers can also reduce the gap between the rollers, thereby reducing liquid backflow and backflushing, improving pumping efficiency, and also means a reduction in pipe pressure, which can reduce pipe wear and fatigue.
[0040] The number of sample bottles 9 is multiple, and the multiple sample bottles 9 are communicated with the input port of the sample pump 3 through the multi-way pipeline.
[0041] A vibration motor is further arranged near the pipeline at the sample mixing pool 12, which is used to shake the liquid at the input pipeline of the sample mixing pool 12 into the sample mixing pool 12, and automatically vibrate the body of the sample mixing pool 12, so as to complete the mixing and dyeing.
[0042] The system uses a switching power supply with a rated power of 200W, an output of 12V / 24V adjustable, and an overload, overvoltage and short circuit protection function, which is sufficient to drive five step motors to work normally. In addition, the system components further include a wiring terminal for wiring, a 12V to 5V voltage reduction module for power supply, an eight-way relay module for controlling the on-off of the circuit, which plays the role of an electrical switch, and a left plate and a bottom plate for fixing.
[0043] Since the liquid needs to be pre-filled in the pipeline before sampling, when performing a routine test, the effect of simple cleaning can be achieved during the process of filling the pipeline with a new sample. The pipeline can also be pulled out for deep cleaning by being inserted into a clean water bottle and pulled back, or by taking advantage of the detachable advantage of the peristaltic pump.
[0044] The microcontroller selects an STM32F103ZET6 chip, which integrates 512KB of Flash memory and 64KB of SRAM, has a maximum working frequency of 72MHz, has rich peripheral interfaces, including up to 112 GPIO pins, 12-bit ADC, DAC, I 2 C, SPI, USART and CAN bus interfaces. In addition, it also supports a USB 2.0 full-speed interface and multiple timers, which are suitable for complex real-time control and communication tasks. The speed of the step motor is planned through software to realize closed-loop driving, further improving the stability of the motor operation. Appropriate software delay in the middle of the steps ensures the full performance of the action flow.
[0045] The system connects to a host computer via a serial port and receives signals from the host computer. During implementation, when mixed sample delivery is required, the host computer sends BCJ1 to indicate the start, and the slave computer sends BCJ2 to indicate the completion of sample delivery. For a single sample delivery, the host computer sends BCJ3 to indicate the start, and the slave computer sends BCJ4 to indicate the completion of the single sample delivery. In the cleaning step, the host computer sends BCJ5 to indicate the start of the cleaning step, and the slave computer sends BCJ6 to indicate the completion of the cleaning process. If a certain process is not cleaned sufficiently, the host computer can send a control signal to individually control the continuation of that process to ensure the completion of the process. The system is equipped with a stroke electromagnet that acts on the waste liquid pipeline with a stroke of 10mm and a maximum push-pull force of 65N. The host computer controls the microcontroller to send a switch signal to the relay control coil to turn it on and off, causing the push rod to move and press against the pipe connected to the observation chamber outlet, ensuring that the liquid does not flow back through the pipeline. Silicone pipes are not prone to fatigue or breakage, can withstand repeated compression and release forces, and have a specially designed stroke electromagnet support for use with the pipes.
[0046] The method of using the tubular automatic sample injection system proposed in this utility model includes:
[0047] The control pipeline-type automatic sampling system performs mixed sample delivery until the sample delivery of the detection area is completed;
[0048] The control system for pipeline-type automatic sample feeding performs pipeline self-cleaning.
[0049] The controlled pipeline-type automatic sample delivery system performs mixed sample delivery until the sample delivery of the detection area is completed, including the following steps:
[0050] Step 1: Check valve 15 is in the OFF state. The host computer sends a start signal, first starting sample pump 3 and dye pump 5 to pump the sample and dye for a period of time until the sample and dye fill their respective pipelines, facilitating subsequent synchronous and adjustable sample injection. Then, the pipeline at the inlet of the mixing tank 12 is vibrated by a vibration motor for 0.5 seconds, causing the liquid at the pipe opening to be shaken into the mixing tank 12. Next, the second cleaning pump 2 is started to inject clean water into the mixing tank 12 and clean the residual liquid. Finally, the second waste liquid pump 7 is started to remove the waste liquid after cleaning.
[0051] Step 2: As Figure 2 As shown, the sample pump 3 and dye pump 5 are started, and the sample and dye are pumped to the mixing tank 12 simultaneously through the pipeline according to the preset ratio (e.g., 4:1). After a 5-second delay to wait for the fluid to stabilize, the microcontroller generates a switching signal to power on the vibration motor rigidly connected to the mixing tank for 5 seconds, and then automatically vibrates and mixes the system in the mixing tank 12 for dyeing.
[0052] Step 3: The loading pump 4 is connected with the mixing pool 12 and the detection area through the pipeline. After the mixing is completed, the state of the switching check valve 15 is switched, specifically, the switching check valve 15 is switched to the ON state, and the microcontroller sends a pulse signal to drive the loading pump 4 to pump the mixed liquid to the detection area at a set rate; the control level of the microcontroller to the driver direction control port of the sample pump 3 and the dye pump 5 is reversed, and the sample pump 3 and the dye pump 5 are driven to suck the small amount of sample and dye remaining in the pipeline, so as to shorten the waiting time for the flow rate to slow down and facilitate photographing and counting. Subsequently, the lower computer sends a sample sending completion signal to the upper computer, and the upper computer can automatically photograph and count.
[0053] Step 4: The state of the switching check valve 15 is switched, that is, the switching check valve 15 is switched to the OFF state, so that the mixing pool 12 is connected with the second waste liquid pump 7, the travel electromagnetic iron is connected, and the outlet pipeline of the observation chamber is clamped to prevent the waste liquid from flowing back, as shown in Figure 3 , the second waste liquid pump 7 is started to pump the excess waste liquid in the mixing pool 12 to the waste liquid bottle 11.
[0054] Step 5: Steps 1-4 are repeated until the sample sending of the detection area is completed. The upper computer sends a single sample sending signal, the travel electromagnetic iron is disconnected and reset, the loading pump 4 is started to send the sample once, then the travel electromagnetic iron is connected, and the lower computer sends a single sample sending completion signal to the upper computer.
[0055] The control pipeline type automatic sample injection system performs pipeline self-cleaning, including:
[0056] Step 1: The upper computer sends a cleaning signal, the second cleaning pump 2 is started to inject clean water into the mixing pool 12, the vibration motor is powered on to vibrate the mixing pool 12 for 2.5 seconds, and then the second waste liquid pump 7 is started to pump the waste liquid in the mixing pool 12 away;
[0057] Step 2: As shown in Figure 4 , the second cleaning pump 2 is started to inject clean water into the mixing pool 12 again, the state of the switching check valve 15 is switched, specifically, the switching check valve 15 is switched to the ON state, the loading pump 4 is started to pump the clean water to the detection area for cleaning the pipeline and the observation chamber, and then the switching check valve 15 is reset, the second waste liquid pump 7 is started to pump the excess waste liquid into the waste liquid bottle 11;
[0058] Step 3: The first cleaning pump 1 is started to quickly inject clean water into the sample bottle 9, and after the completion, the first waste liquid pump 6 is started to pump the waste liquid in the sample bottle 9 into the waste liquid bottle 11.
[0059] Step 4: The control level of the microcontroller to the driver direction control port of the sample pump 3, the dye pump 5 and the second cleaning pump 2 is reversed, and the first cleaning pump 1, the sample pump 3, the dye pump 5 and the second cleaning pump 2 are driven to suck the small amount of liquid remaining in the pipeline. Subsequently, the lower computer sends a cleaning completion signal.
[0060] The above steps ensure that the automatic sampling, mixing, dyeing, detection and cleaning process of the system is efficient, accurate and reliable. The main idea of the system is that the microcontroller communicates with the upper computer, controls the electromagnetic valve, motor, vibration motor and other components in the system through the control signal of the upper computer, and then realizes the functions of automatic sampling, automatic dyeing vibration, automatic sampling and automatic cleaning.
[0061] Finally, it should be noted that the above examples and explanations are only used to illustrate the technical solutions of the present application and are not limiting. Those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions disclosed in the present application. They should be covered in the protection scope of the claims of the present application.
Claims
1. A tubular automatic sample introduction system, characterized in that, The system includes a second cleaning pump (2), a sample pump (3), a feed pump (4), a dye pump (5), a second waste liquid pump (7), a water bottle (8), a sample bottle (9), a dye bottle (10), a waste liquid bottle (11), a mixing tank (12), and a control module. The inlet of the second cleaning pump (2) is connected to the water bottle (8), and the outlet of the second cleaning pump (2) is connected to the inlet of the mixing tank (12). The inlet of the sample pump (3) is connected to the sample bottle (9), and the outlet of the sample pump (3) is connected to the inlet of the mixing tank (12). The outlet of the mixing tank (12) is connected to the opening valve via a check valve (15). The valves are connected, the inlet of the feed pump (4) is connected to the outlet of the mixing tank (12) through the switch valve, and the outlet of the feed pump (4) is connected to the detection area; the inlet of the dye pump (5) is connected to the dye bottle (10), and the outlet of the dye pump (5) is connected to the inlet of the mixing tank (12); the inlet of the second waste liquid pump (7) is connected to the outlet of the mixing tank (12) through the switch valve, and the outlet of the second waste liquid pump (7) is connected to the waste liquid bottle (11); the second cleaning pump (2), sample pump (3), feed pump (4), dye pump (5), and second waste liquid pump (7) are all connected to the control module.
2. The tubular automatic sample introduction system according to claim 1, characterized in that, It also includes a first cleaning pump (1) and a first waste liquid pump (6). The inlet of the first cleaning pump (1) is connected to the water bottle (8), and the outlet of the first cleaning pump (1) is connected to the sample bottle (9). The inlet of the first waste liquid pump (6) is connected to the sample bottle (9), and the outlet of the first waste liquid pump (6) is connected to the waste liquid bottle (11).
3. The tubular automatic sample introduction system according to claim 1, characterized in that, The switching valve includes a two-position three-way valve.
4. The tubular automatic sample introduction system according to claim 1, characterized in that, The detection area is the observation chamber within the microfluidic chip.
5. The tubular automatic sample introduction system according to claim 2, characterized in that, The first cleaning pump (1), the second cleaning pump (2), the sample pump (3), the feed pump (4), the dye pump (5), the first waste liquid pump (6), and the second waste liquid pump (7) include one of a six-roller peristaltic pump head or a four-roller peristaltic pump head.
6. The tubular automatic sample introduction system according to claim 1, characterized in that, A vibration motor is also installed near the pipeline at the mixing tank (12) to shake the liquid at the input pipeline of the mixing tank (12) into the mixing tank (12) and to automatically vibrate the body of the mixing tank (12).
7. The tubular automatic sample introduction system according to claim 1, characterized in that, The second cleaning pump (2), sample pump (3), feed pump (4), dye pump (5), and second waste liquid pump (7) are each equipped with a corresponding stroke electromagnet. The control module is electrically connected to all stroke electromagnets to realize the control of the second cleaning pump (2), sample pump (3), feed pump (4), dye pump (5), and second waste liquid pump (7).
8. The tubular automatic sample introduction system according to claim 1, characterized in that, The sample bottle (9) includes a milk tank.
9. The tubular automatic sample introduction system according to claim 1, characterized in that, The number of sample bottles (9) is multiple, and the multiple sample bottles (9) are connected to the input port of the sample pump through a multi-port pipe.
10. A tubular automatic sample introduction system according to claim 1, characterized in that, The control module includes a microcontroller and a host computer. The second cleaning pump (2), sample pump (3), feed pump (4), dye pump (5), and second waste liquid pump (7) are connected to the host computer through the microcontroller.