Nano antibody protein electrophoresis apparatus convenient to operate and use

Abstract

The utility model discloses a nano antibody protein electrophoresis apparatus convenient to operate and use, which relates to the field of electrophoresis apparatuses and comprises a shell, conductive mechanisms are uniformly arranged on the front side wall and the rear side wall of an inner cavity of the shell, a lead is arranged between every two conductive mechanisms on the front side and the rear side, and the conductive mechanisms on the front side and the rear side are arranged in a mirror image manner. According to the scheme, through cooperation of the shell, the wire, the fixing frame and other devices, the rubber frame for detection can be conveniently installed and fixed through the device, poor contact caused by damage due to friction is avoided, electric power conduction is facilitated, the detection efficiency is improved, and the service life of the rubber frame is prolonged. Electrophoresis operation is facilitated; through cooperation of the cover plate, the connecting frame, the pressing frame and other devices, the positive electrode circuit can be disconnected after the cover plate is opened, so that the operation of taking without power failure is avoided, and the risk of electric shock is reduced.

Description

Technical Field

[0001] This utility model belongs to the field of electrophoresis instrument technology, specifically a nano-antibody protein electrophoresis instrument that is easy to operate and use. Background Technology

[0002] An electrophoresis apparatus is an instrument used for electrophoretic analysis. Electrophoresis is a phenomenon in which charged molecules move towards electrodes with opposite charges in an electric field. The technique of separating substances using electrophoresis is called electrophoresis technology. Proteins, as active macromolecules related to diseases, play an important role in disease diagnostic research due to changes in their expression or modification. Nanobodies have become sophisticated tools capable of freezing dynamic proteins into single-function conformations. For the identification of proteins and their intracellular localization, application number 202122205042.7 discloses an easy-to-operate serum protein electrophoresis apparatus, including an electrophoresis apparatus body. The electrophoresis apparatus body includes a cover plate, a box, clamps, copper sheets, and support plates. The top of the box has rotating... The device has a shaft, a cover plate installed on one side of the shaft, a magnet installed on the inner side of the cover plate, a stop bar installed on the other side of the shaft, and a support plate embedded at the bottom of the electrophoresis tank. This easy-to-operate serum protein electrophoresis instrument has high safety, avoids the risk of electric shock, has higher electrophoresis efficiency, and is simple and quick to handle. However, friction occurs between the conductive parts of the device when installing the protein-containing gel frame, which is prone to wear after long-term use and may lead to poor conductive contact, which is not conducive to subsequent nanobody electrophoresis. Therefore, we propose an easy-to-operate nanobody protein electrophoresis instrument. Utility Model Content

[0003] In order to overcome the shortcomings of the prior art, this utility model provides a nanobody protein electrophoresis instrument that is easy to operate and use. It effectively solves the problem that friction occurs between the conductive parts of the device when installing the gel frame containing protein, which is prone to wear after long-term use and may lead to poor conductive contact, which is not conducive to subsequent nanobody electrophoresis operations.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a user-friendly nanobody protein electrophoresis apparatus, comprising a shell, conductive mechanisms evenly arranged on the front and rear sidewalls of the inner cavity of the shell, wires arranged between each pair of the conductive mechanisms on the front and rear sides, the conductive mechanisms on the front and rear sides being mirror images of each other, the rear conductive mechanism including a fixing frame, a conductive sheet arranged on the rear sidewall of the inner cavity of the fixing frame, the left and right sidewalls of the conductive sheet respectively penetrating the left and right sidewalls of the fixing frame, a conductive spring arranged on the front sidewall of the fixing frame, a conductive frame arranged on the inner wall of the fixing frame, a conductive roller arranged on the inner wall of the conductive frame, and the conductive sheet connected to the wires.

[0005] Preferably, a cover plate is provided on the rear side of the top wall of the outer shell, a connecting frame is provided on the rear side wall of the inner cavity of the outer shell, electric contact pieces are provided on both the left and right sides of the bottom wall of the inner cavity of the connecting frame, a tension spring is provided on the top wall of the inner cavity of the connecting frame, a pressing frame is provided at the bottom end of the tension spring, an electric contact piece is provided on the bottom wall of the pressing frame, and the top end of the pressing frame penetrates the top wall of the connecting frame.

[0006] Preferably, the bottom wall of the inner cavity of the outer shell is uniformly provided with clamping frames, the clamping frames are arranged in a U-shape, and the clamping frames correspond to the conductive mechanism.

[0007] Preferably, the outer wall of the conductive roller is provided with an annular groove, and the annular groove matches the width of the clamping frame.

[0008] Preferably, the front side of the top wall of the outer shell is provided with a mounting groove, the inner wall of the mounting groove is provided with a snap-fit ​​bracket, the snap-fit ​​bracket is L-shaped, the front side wall of the inner cavity of the mounting groove is provided with a push spring, the rear end of the push spring is provided on the front side wall of the snap-fit ​​bracket, the front side wall of the snap-fit ​​bracket is provided with a pull rod, and the front end of the pull rod penetrates the front side wall of the outer shell.

[0009] Compared with the prior art, the beneficial effects of this utility model are:

[0010] 1. This easy-to-operate nanobody protein electrophoresis instrument, through the combination of a shell, wires, and a fixing frame, allows for easy installation and fixation of the gel frame used for detection, avoiding damage from friction that could lead to poor contact, facilitating power conduction, and making electrophoresis operations easier;

[0011] 2. This easy-to-use nanobody protein electrophoresis instrument, through the combination of a cover plate, connecting frame, and pressing frame, can disconnect the positive electrode circuit after the cover plate is opened, thereby avoiding the operation of taking it out without disconnecting the power and reducing the risk of electric shock.

[0012] 3. This easy-to-operate nanobody protein electrophoresis apparatus, through the combination of clamping plates, outer shell and conductive rollers, can easily clamp and fix the gel frame, prevent the gel frame from shifting, and facilitate electrophoresis operation by energizing the gel frame. Attached Figure Description

[0013] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0014] In the attached diagram:

[0015] Figure 1 This is a schematic diagram of the structure of the nano-antibody protein electrophoresis apparatus of this utility model, which is easy to operate and use.

[0016] Figure 2 This is a schematic diagram of the structure of the clamping frame of this utility model;

[0017] Figure 3 This utility model Figure 2 Enlarged view of part A in the image;

[0018] Figure 4 This is a cross-sectional view of the fixing frame of this utility model;

[0019] Figure 5 This is a cross-sectional view of the connecting frame of this utility model.

[0020] In the diagram: 100, outer casing; 101, conductive mechanism; 102, wire; 103, fixing frame; 104, conductive sheet; 105, conductive spring; 106, conductive frame; 107, conductive roller; 108, cover plate; 109, connecting frame; 110, contact sheet; 111, tension spring; 112, pressing frame; 113, energized sheet; 114, clamping frame; 115, snap-fit ​​frame; 116, pushing spring; 117, pull rod. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0022] Depend on Figure 1 , Figure 2 and Figure 3This invention discloses an easy-to-operate nanobody protein electrophoresis apparatus. Conductive mechanisms 101 are uniformly fixedly installed on the front and rear side walls of the inner cavity of the outer shell 100. Wires 102 are fixedly connected between each pair of the front and rear conductive mechanisms 101, facilitating communication between them. The front and rear conductive mechanisms 101 are arranged in a mirror image. The rear conductive mechanism 101 includes a fixing frame 103, which supports the conductive sheet 104. The fixing frame 103 is made of insulating material, and the conductive sheet 104 is fixedly connected to the rear side wall of the inner cavity of the fixing frame 103, facilitating communication between them. For conducting electricity, the left and right sidewalls of the conductive sheet 104 pass through the left and right sidewalls of the fixing frame 103 respectively. A conductive spring 105 is fixedly connected to the front sidewall of the fixing frame 103, which facilitates pushing the conductive frame 106. The conductive frame 106 is slidably connected to the inner wall of the fixing frame 103, which facilitates supporting the conductive roller 107. The outer wall of the conductive frame 106 is square to prevent the conductive frame 106 from deflecting. The conductive roller 107 is rotatably connected to the inner wall of the conductive frame 106, and the conductive roller 107 rolls on the rubber frame. The conductive sheet 104 is connected to the wire 102.

[0023] In this embodiment: The easy-to-operate serum protein electrophoresis apparatus is powered by an external power source. The power supply line is connected to the conductive mechanism 101. First, the gel frame containing nano-antibody protein is inserted sequentially into the middle of the front and rear conductive rollers 107. The electric current is conducted through the conductive sheet 104, conductive spring 105, conductive frame 106, and conductive rollers 107. The conductive spring 105 facilitates pushing the conductive frame 106, and the conductive frame 106 pushes the conductive rollers 107, making the conductive rollers 107 contact the gel frame. The rolling of the conductive rollers 107 avoids friction and damage, thus enabling electrophoresis of the nano-antibody protein solution inside each gel frame. This makes it easy to install and fix the gel frame for detection, avoids friction damage leading to poor contact, facilitates electric current conduction, and facilitates electrophoresis operations.

[0024] A cover plate 108 is rotatably connected to the rear side of the top wall of the outer casing 100, which facilitates the sealing of the outer casing 100. A connecting frame 109 is fixedly connected to the rear side wall of the inner cavity of the outer casing 100, which facilitates the support of the contact piece 110. Contact pieces 110 are fixedly connected to both the left and right sides of the bottom wall of the inner cavity of the connecting frame 109, which facilitates the conduction of electricity. A tension spring 111 is fixedly connected to the top wall of the inner cavity of the connecting frame 109, which facilitates the pulling of the pressing frame 112 to reset. The bottom end of the tension spring 111 is fixedly connected to the pressing frame 112, which facilitates the movement of the energized piece 113. The bottom wall of the pressing frame 112 is fixedly connected to the energized piece 113, which facilitates the connection of the two sets of contact pieces 110. The top end of the pressing frame 112 penetrates the top wall of the connecting frame 109.

[0025] In this embodiment: the positive terminal of the power supply is connected to the left contact piece 110, and the conductive mechanism 101 is connected to the right contact piece 110. The pressing frame 112 is pushed by the closed cover plate 108, and the pressing frame 112 drives the energized piece 113 to move. The energized piece 113 moves to contact the two sets of contact pieces 110, so that the circuit is connected. After the cover plate 108 is opened, the pressing frame 112 is pulled by the elastic force of the tension spring 111, so that the pressing frame 112 rises and drives the energized piece 113 to rise and disconnect the circuit. This allows the device to disconnect the positive circuit after the cover plate 108 is opened, thereby avoiding the operation of taking it without disconnecting the power and reducing the risk of electric shock.

[0026] The bottom wall of the inner cavity of the outer shell 100 is uniformly fixedly connected with a clamping frame 114. The clamping frame 114 facilitates the support and limiting of the rubber frame. The clamping frame 114 is U-shaped and corresponds to the conductive mechanism 101.

[0027] In this embodiment: when placing the glue frame, it is inserted into the inner cavity of the clamping frame 114. The clamping frame 114 facilitates the support and limiting of the glue frame, which makes it easy for the device to clamp and fix the glue frame, avoids the glue frame from shifting, and is conducive to the glue frame being energized for electrophoresis.

[0028] The outer wall of the conductive roller 107 is provided with an annular groove, which matches the width of the clamping frame 114.

[0029] In this embodiment: the ring groove facilitates the guiding and limiting of the rubber frame, and facilitates the limiting and support of the rubber frame. It also cooperates with the clamping plate frame 114 to better support and limit the rubber frame.

[0030] A mounting groove is provided on the front side of the top wall of the outer casing 100. A snap-fit ​​bracket 115 is slidably connected to the inner wall of the mounting groove. The snap-fit ​​bracket 115 is used to limit the position of the cover plate 108. The snap-fit ​​bracket 115 is L-shaped. A push spring 116 is fixedly connected to the front side wall of the inner cavity of the mounting groove. The push spring 116 is used to push the snap-fit ​​bracket 115. The rear end of the push spring 116 is fixedly connected to the front side wall of the snap-fit ​​bracket 115. A pull rod 117 is fixedly connected to the front side wall of the snap-fit ​​bracket 115. The pull rod 117 is used to move the snap-fit ​​bracket 115. The front end of the pull rod 117 passes through the front side wall of the outer casing 100.

[0031] In this embodiment: the snap-fit ​​bracket 115 facilitates the snap-fit ​​of the cover plate 108, preventing the cover plate 108 from tilting up and affecting the circuit connection. By pulling the lever 117, the snap-fit ​​bracket 115 is moved, and the snap-fit ​​bracket 115 is released from the snap-fit ​​of the cover plate 108, making it easier for the cover plate 108 to open and close, which is beneficial for the operator.

Claims

1. A user-friendly nanobody protein electrophoresis apparatus, comprising a housing (100), characterized in that: Conductive mechanisms (101) are evenly arranged on the front and rear sidewalls of the inner cavity of the outer shell (100). Wires (102) are arranged between each pair of the conductive mechanisms (101) on both the front and rear sides. The conductive mechanisms (101) on both the front and rear sides are arranged in a mirror image. The rear conductive mechanism (101) includes a fixing frame (103). A conductive sheet (104) is arranged on the rear sidewall of the inner cavity of the fixing frame (103). The left and right sidewalls of the conductive sheet (104) respectively penetrate the left and right sidewalls of the fixing frame (103). A conductive spring (105) is arranged on the front sidewall of the fixing frame (103). A conductive frame (106) is arranged on the inner wall of the fixing frame (103). A conductive roller (107) is arranged on the inner wall of the conductive frame (106). The conductive sheet (104) is connected to the wires (102).

2. The easy-to-operate nanobody protein electrophoresis apparatus according to claim 1, characterized in that: A cover plate (108) is provided on the rear side of the top wall of the outer shell (100). A connecting frame (109) is provided on the rear side wall of the inner cavity of the outer shell (100). Electric contact pieces (110) are provided on both the left and right sides of the bottom wall of the inner cavity of the connecting frame (109). A tension spring (111) is provided on the top wall of the inner cavity of the connecting frame (109). A pressing frame (112) is provided at the bottom end of the tension spring (111). An electric contact piece (113) is provided on the bottom wall of the pressing frame (112). The top end of the pressing frame (112) penetrates the top wall of the connecting frame (109).

3. The easy-to-operate nanobody protein electrophoresis apparatus according to claim 2, characterized in that: The bottom wall of the inner cavity of the outer shell (100) is uniformly provided with clamping frames (114), which are U-shaped and correspond to the conductive mechanism (101).

4. The easy-to-operate nanobody protein electrophoresis apparatus according to claim 3, characterized in that: The outer wall of the conductive roller (107) is provided with an annular groove, which matches the width of the clamping frame (114).

5. The easy-to-operate nanobody protein electrophoresis apparatus according to claim 4, characterized in that: The front side of the top wall of the outer shell (100) is provided with an installation groove. The inner wall of the installation groove is provided with a snap-fit ​​bracket (115). The snap-fit ​​bracket (115) is L-shaped. The front side wall of the inner cavity of the installation groove is provided with a push spring (116). The rear end of the push spring (116) is provided on the front side wall of the snap-fit ​​bracket (115). The front side wall of the snap-fit ​​bracket (115) is provided with a pull rod (117). The front end of the pull rod (117) penetrates the front side wall of the outer shell (100).

Images