Device for anaerobic collection and cryopreservation of excrement

Abstract

The utility model relates to the technical field of excrement sample storage and cryopreservation, and particularly discloses a device for excrement anaerobic collection and cryopreservation, which comprises a storage device, an anaerobic collection device and a cryopreservation device, the cover body is hermetically arranged at the upper end of the storage device and is used for storing the anaerobic bag; the fixing plate is located on the inner side of the storage device; the adjusting plate is located on the lower portion of the fixing plate, the anaerobic bag is arranged, the anaerobic bag releases chemical substances into the storage device, oxygen in the device is exhausted, and the anaerobic bag is used for creating an anaerobic environment in the device, so that the activity of anaerobic bacteria in panda excrement in the sample transportation process is maintained, and the separation rate and separation diversity of the anaerobic bacteria in the later period are improved; an arc-shaped pointed end is arranged in the storage device, the sample shape is met, the sample is prevented from being extruded, in addition, the adjusting plate can also control the space size of the storage area, the situation that the sample is shaken during transportation due to the too large space is prevented, and the effect of isolating the anaerobic bag from the sample can also be achieved.

Description

Technical Field

[0001] This utility model relates to the field of fecal sample storage and cryopreservation technology, specifically, to a device for anaerobic collection and cryopreservation of feces. Background Technology

[0002] Giant pandas are among the world's rarest and most endangered wild animals, and a flagship species in conservation biology. Giant panda feces are spindle-shaped and mainly composed of bamboo leaves and stalk residue. A mucous membrane covers the surface of the feces, containing cells shed from the intestinal wall. This membrane can be used to extract giant panda DNA, making it an important material for giant panda conservation genetics research.

[0003] During sample collection, maintaining the integrity of the fecal surface and obtaining as much of the fecal surface mucosa as possible helps improve the quality of DNA extraction. To reduce environmental contamination of the feces, fresh and intact feces are typically collected, and after peeling off the surface layer, samples are taken from the central part of the feces for research.

[0004] The use of sterile sampling bags in the present technology has the following disadvantages: When collecting giant panda feces using sterile sampling bags, the feces are easily crushed during sampling and transportation, resulting in damage to the surface mucosal layer and reducing the mucosal layer acquisition rate. In addition, because the feces are spindle-shaped, it is not easy to place them neatly when storing feces in an ultra-low temperature freezer using traditional sampling bags. When samples are stacked, they are easily crushed, resulting in damage to the surface mucosal layer and reducing the mucosal layer acquisition rate. Most importantly, the existing sampling bags cannot effectively expel the air in the bag to achieve an anaerobic environment. There is no anaerobic collection device specifically for giant panda feces, which cannot meet the needs of anaerobic bacteria research in the giant panda intestine (feces). Utility Model Content

[0005] The purpose of this invention is to provide a device for anaerobic collection and freezing of feces, thereby solving the aforementioned technical problems.

[0006] This utility model provides the following technical solution: a device for anaerobic collection and freezing of feces, comprising:

[0007] Storage device, containing samples;

[0008] A lid, sealed at the top of the storage device, is used to store anaerobic bags;

[0009] A fixing plate is located inside the storage device and has holes inside;

[0010] The adjusting plate is located at the lower part of the fixing plate, and a hole is formed in the adjusting plate, so that the chemical substance in the anaerobic bag can pass through the hole and enter the area for storing the sample, and the chemical substance in the anaerobic bag reacts with the oxygen in the storage device through the hole of the two plate bodies, so that the sample in the storage device is kept in an anaerobic environment.

[0011] As a preferred technical scheme of the above technical scheme, a sealing ring is further arranged between the storage device and the cover.

[0012] As a preferred technical scheme of the above technical scheme, the cover comprises a top cover sealingly arranged on the storage device, and a sealing cover is hingedly arranged on the inner side of the top cover and used for opening, and a protruding part is further fixedly arranged on the upper part of the sealing cover.

[0013] As a preferred technical scheme of the above technical scheme, a storage area for arranging the anaerobic bag is formed in the top cover.

[0014] As a preferred technical scheme of the above technical scheme, the sealing cover is made of silica gel.

[0015] As a preferred technical scheme of the above technical scheme, the storage device comprises a shell, the fixing plate is arranged at the upper end of the inner side of the shell, the anaerobic bag is arranged on the upper surface of the fixing plate, and the fixing plate can be detached from the shell.

[0016] As a preferred technical scheme of the above technical scheme, the storage device and the cover are both made of a low-temperature-resistant material.

[0017] As a preferred technical scheme of the above technical scheme, a pointed end is integrally formed on the inner wall of the shell, a storage area is formed between the adjusting plate and the pointed end, and the shape of the sample is adapted.

[0018] As a preferred technical scheme of the above technical scheme, the adjusting plate comprises a plate body, a threaded part is fixedly arranged on the peripheral side of the plate body, a threaded groove is formed in the shell corresponding to the threaded part, the plate body is screwed to the inner side of the shell with the threaded groove through the threaded part, and a rotating part is fixedly arranged on the upper surface of the plate body.

[0019] As a preferred technical scheme of the above technical scheme, a stand is integrally arranged at the lower end of the pointed end, and an identification area is arranged on the outer side of the stand.

[0020] Compared with the prior art, the present application has the following beneficial effects:

[0021] In this invention, an anaerobic bag is provided. The anaerobic bag releases chemical substances into the storage device, depleting the oxygen in the device and creating an anaerobic environment. This helps maintain the activity of anaerobic bacteria in giant panda feces during sample transportation, improving the separation rate and diversity of anaerobic bacteria in the later stages. The storage device also has an arc-shaped pointed end to accommodate the sample shape and prevent the sample from being squeezed. In addition, the adjustment plate can control the size of the storage area to prevent the sample from shaking during transportation due to excessive space, and it also serves to isolate the anaerobic bag from the sample. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0023] Figure 2 for Figure 1 A schematic diagram of the cross-sectional structure;

[0024] Figure 3 for Figure 1 A schematic diagram of the decomposed structure;

[0025] Figure 4 A schematic diagram of the overall structure of the adjustment plate is provided.

[0026] Figure 5 A schematic diagram illustrating the working state of the device of this utility model is provided.

[0027] In the diagram: 100, storage device; 200, cover; 300, sealing ring; 400, anaerobic bag; 500, sample; outer shell; 120, fixing plate; 130, adjusting plate; 140, stand; 150, storage area; 160, storage area; 110-1, threaded groove; 110-2, pointed end; 130-1, plate; 130-2, rotating part; 130-3, threaded part; 210, top cover; 220, sealing cover; 230, protrusion. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] like Figure 1 As shown, the technical solution provided by this utility model is: a device for anaerobic collection and cryopreservation of giant panda samples, including a storage device 100, and a cover 200 detachably connected to the upper end of the storage device 100, with the cover 200 and the storage device 100 being sealed together.

[0030] As shown in Figure 2 , the storage device 100 comprises a shell 110, two groups of hole-covered cover plates are arranged on the inner side of the shell 110 respectively, which are a fixed plate 120 and an adjusting plate 130, and a storage area 150 is further formed on the inner side of the cover body 200, and an anaerobic bag 400 as shown in Figure 5 is stored in the fixed plate 120, and the adjusting plate 130 can also be detached from the inner side of the shell 110, which is used for putting the sample 500 into the storage area 160, and a pointed end 110-2 is integrally formed on the inner wall of the shell 110, and the storage area 160 is formed between the adjusting plate 130 and the pointed end 110-2, which is used for storing the sample 500 as shown in Figure 5 , which is used for adapting to the sample 500 in the shape of panda spindle.

[0031] As shown in Figure 2 , the lower end of the pointed end 110-2 is further integrally provided with a stand 140, and the outer wall of the stand 140 is further provided with an identification area 140-1, which is used for filling in the basic information of the sample 500, so as to facilitate the traceability management in the later period.

[0032] The inner wall of the shell 110 is smooth, which is used for storing the sample 500;

[0033] As shown in Figure 5 , after the sample is put in, the adjusting plate 130 and the fixed plate 120 are put into the shell 110 again, and then the anaerobic bag 400 is put in, the main principle of the anaerobic bag 400 is to create an anaerobic environment in the storage device 100 through chemical reaction, specifically, the anaerobic bag 400 uses sodium borohydride to react with water to produce hydrogen gas, and the hydrogen gas combines with the oxygen in the bag under the action of the catalyst palladium to generate water, so as to achieve the purpose of oxygen removal, in addition, the reaction of citric acid and sodium bicarbonate will produce carbon dioxide, which is beneficial to the growth of anaerobic bacteria which needs carbon dioxide, Figure 5 The anaerobic bag 400 shown in evolves upward and downward, removes the oxygen in the cover body 200 upward to prevent it from flowing into the storage device 100, so that the sample 500 in the storage device 100 is in an anaerobic environment, which meets the needs of the research of anaerobic bacteria in the intestinal tract (feces) of giant pandas.

[0034] In order to ensure the sealing performance, as shown in Figure 3 , a sealing ring 300 is further arranged between the cover body 200 and the storage device 100, in addition, in order to facilitate the storage and taking out of the anaerobic bag 400, the cover body 200 further comprises a top cover 210, the inner side of the upper end of the top cover 210 is hinged with a sealing cover 220, the sealing cover 220 is made of silica gel material, which enhances the sealing performance, and the outer surface of the sealing cover 220 is further integrally formed with a protruding part 230, the collector pinches the protruding part 230 to open or close the sealing cover 220.

[0035] To prevent the storage device 100 from becoming too large and causing the stored sample 500 to shake within it, a mechanism is provided as follows: Figure 4 The adjusting plate 130 includes a plate body 130-1, a rotating part 130-2 fixedly installed on the upper surface of the plate body 130-1, and a threaded part 130-3 fixedly provided on the periphery of the plate body 130-1. A threaded groove 110-1 is correspondingly provided along the inner side of the outer shell 110 to cooperate with the plate body 130-1 with the threaded part 130-3. The plate body 130-1 can be rotated along the inner side of the outer shell 110 to adjust its position up and down to accommodate the size of the sample 500. Secondly, the adjusting plate 130 is also significant in that it can provide secondary isolation for the anaerobic bag 400.

[0036] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this utility model, and these should all be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A device for anaerobic collection and cryopreservation of feces, characterized in that, The utility model relates to an anaerobic storage device, which comprises: a storage device (100) internally storing a sample (500); a cover (200) sealingly arranged at the upper end of the storage device (100) for storing an anaerobic bag (400); a fixing plate (120) arranged inside the storage device (100) and having a hole formed therein; an adjusting plate (130) arranged at the lower part of the fixing plate (120) and having a hole formed therein, so that the chemical substance in the anaerobic bag (400) can pass through the hole and react with the oxygen in the storage device (100), thereby keeping the sample (500) in the storage device (100) in an anaerobic environment.

2. The device for anaerobic collection and cryopreservation of feces according to claim 1, characterized in that: A sealing ring (300) is further arranged between the storage device (100) and the cover (200).

3. The device for anaerobic collection and cryopreservation of fecal material of claim 1, wherein: The cover (200) comprises a top cover (210) sealingly arranged on the storage device (100), and a sealing cover (220) hingedly connected to the inner side of the top cover (210) for opening, and a protruding part (230) fixedly arranged at the upper part of the sealing cover (220).

4. The device for anaerobic collection and cryopreservation of feces according to claim 3, characterized in that: The top cover (210) is formed with a storage area (150) for accommodating the anaerobic bag (400).

5. The device for anaerobic collection and cryopreservation of fecal material of claim 3, wherein: The sealing cover (220) is made of silica gel.

6. The device for anaerobic collection and cryopreservation of fecal material of claim 1, wherein: The storage device (100) comprises an outer shell (110), the fixing plate (120) is arranged at the inner side of the outer shell (110) at the upper end, the anaerobic bag (400) is arranged on the upper surface of the fixing plate (120), and the fixing plate (120) can be detached from the outer shell (110).

7. The device for anaerobic collection and cryopreservation of fecal matter of claim 1, wherein: The storage device (100) and the cover (200) are made of low-temperature resistant material.

8. The device for anaerobic collection and cryopreservation of fecal material of claim 6, wherein: The inner wall of the outer shell (110) is further integrally formed with a pointed end (110-2), and a storage area (160) is formed between the adjusting plate (130) and the pointed end (110-2) for adapting to the shape of the sample (500).

9. The device for anaerobic collection and cryopreservation of fecal material according to claim 8, characterized in that: The adjusting plate (130) comprises a plate body (130-1), the plate body (130-1) is further fixedly provided with a threaded part (130-3) at the side, the outer shell (110) is provided with a threaded groove (110-1) corresponding to the threaded part (130-3), the plate body (130-1) is screw-connected to the inner side of the outer shell (110) with the threaded groove (110-1) through the threaded part (130-3), and the upper surface of the plate body (130-1) is further fixedly provided with a rotating part (130-2).

10. The device for anaerobic collection and cryopreservation of fecal material of claim 9, wherein: The pointed end (110-2) is further integrally provided with a stand (140) at the lower end, and the outer side of the stand (140) is further provided with an identification area (140-1).

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