Culture dish for prothallium

By designing a culture dish with a water-addition component and a breathable membrane component, the problems of pollution and difficulty in controlling the amount of water added during the existing culture dish are solved, realizing pollution-free quantitative water addition and gas exchange, and ensuring the smooth progress of the fertilization process of ferns.

CN223879736UActive Publication Date: 2026-02-06INSTITUTE OF TCM HEALTH INDUSTRY CACMS +1
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Patent Information

Application Number
CN202422868844.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-25
Publication Date
2026-02-06
Estimated Expiration
2034-11-25

AI Technical Summary

Technical Problem

Existing petri dishes are prone to contaminating the prothallus when water is added, and the amount of water added cannot be controlled, which affects the fertilization process of ferns.

Method used

A culture dish comprising a water addition component and a breathable membrane component was designed. Water is added quantitatively and without contamination through the water addition tube and the breathable membrane. The breathable membrane component allows air to pass through to maintain gas exchange inside the dish.

Benefits of technology

This method enables the precise addition of water without contaminating the prothallus, maintaining gas exchange within the dish and ensuring the smooth progress of the fertilization process in ferns.

✦ Generated by Eureka AI based on patent content.

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Abstract

The culture dish comprises a dish body and a dish cover, the dish cover is detachably arranged on the dish body, and two through holes are formed in the dish cover at intervals; the water adding assembly comprises a water adding pipe, an upper blocking piece, a connecting piece and a lower blocking piece, a plurality of water drainage holes are formed in the water adding pipe, the water adding pipe is detachably connected with one of the through holes, the upper blocking piece is connected with the lower blocking piece through the connecting piece, a baffle is arranged on the lower blocking piece, and the water drainage holes are formed in the water adding pipe. The upper blocking piece is detachably connected with the water adding pipe, and the lower blocking piece can slide in the water adding pipe; and the gas-permeable membrane assembly is connected with the water adding pipe and is arranged above the other through hole. According to the pteridophyte prothallus culture vessel, the inside of the vessel body can be ventilated with the outside under the condition that the vessel body is not polluted, water can be quantitatively added irregularly, and the fertilization rate in the pteridophyte prothallus culture process is increased.
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Description

TECHNICAL FIELD

[0001] The utility model relates to a culture dish technical field especially a culture dish for prothallus. BACKGROUND

[0002] As the container of culture medium, the culture dish has been used in the field of cell or microorganism culture for decades, and is often used in laboratories. The culture dish is usually circular, and is composed of a dish body containing culture medium and a matching dish cover. During the cultivation of the prothallus of fern in the culture dish, water needs to be added regularly to help the sperm of fern swim to the archegonia and complete the process of fertilization. The existing culture dish needs to remove the dish cover to add water, which can easily contaminate the prothallus and cannot control the amount of water added, which has disadvantages. SUMMARY

[0003] The utility model discloses a culture dish for prothallus to solve the technical problem.

[0004] The utility model discloses a culture dish for prothallus, including dish body and dish cover, the dish cover detachably arranged on dish body, the dish cover is equipped with two through -holes at intervals on, still include:

[0005] Water adding assembly, including water adding pipe, upper stop piece, connecting piece and lower stop piece, be equipped with a plurality of drainage holes on water adding pipe, water adding pipe with one through -hole detachable connection, upper stop piece passes through connecting piece and lower stop piece are connected, be equipped with baffle on lower stop piece, upper stop piece with water adding pipe detachable connection, lower stop piece can slide in water adding pipe;

[0006] Breathable membrane assembly, with water adding pipe connection setting above another through -hole.

[0007] Preferably, the upper stop piece is threadedly connected with the water adding pipe.

[0008] Preferably, the connecting piece is an elastic connecting piece, and the upper stop piece is heavier than the lower stop piece.

[0009] Preferably, the breathable membrane assembly includes a connecting rod and a breathable membrane, the connecting rod has a first air hole, and the breathable membrane is arranged at the first air hole.

[0010] Preferably, the first air hole is a countersunk hole, and the breathable membrane assembly further includes a pressing plate having an annular groove and a second air hole, and the pressing plate is detachably connected with the first air hole on the connecting rod.

[0011] The utility model discloses a culture dish for prothallus, including dish body and dish cover, the dish cover detachably arranged on dish body, the dish cover is equipped with two through -holes at intervals on, still include: The utility model discloses a culture dish for prothallus to solve the technical problem. Attached Figure Description

[0012] Figure 1 This is a cross-sectional view of the overall structure of a preferred embodiment of the present invention;

[0013] Figure 2 yes Figure 1 Enlarged view of a portion of point A in the middle;

[0014] Figure 3 This is a schematic diagram of the water inlet pipe in a preferred embodiment of the present invention;

[0015] Figure 4 This is a schematic diagram of the lower plug in a preferred embodiment of the present invention;

[0016] Figure 5 This is a cross-sectional view of the connecting rod in a preferred embodiment of the present invention;

[0017] Figure 6 This is a schematic diagram of the pressure plate in a preferred embodiment of the present invention.

[0018] Reference numerals: 10 for dish body, 2 for dish lid, 201 for through hole, 3 for water inlet pipe, 301 for drain hole, 4 for upper plug, 5 for connector, 6 for lower plug, 601 for baffle, 7 for connecting rod, 701 for first vent, 8 for breathable membrane, 9 for pressure plate, 901 for annular groove, and 902 for second vent. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Reference Figures 1 to 6 A culture dish for prothallus includes a dish body 10 and a dish lid 2, wherein the dish lid 2 is detachably mounted on the dish body 10, and the dish lid 2 has two through holes 201 spaced apart; it also includes:

[0021] A water filling assembly includes a water filling pipe 3, an upper plug 4, a connector 5, and a lower plug 6. The water filling pipe 3 has several drain holes 301, and the water filling pipe 3 is detachably connected to one of the through holes 201. The upper plug 4 is connected to the lower plug 6 through the connector 5. The lower plug 6 has a baffle 601. The upper plug 4 is detachably connected to the water filling pipe 3, and the lower plug 6 can slide inside the water filling pipe 3.

[0022] The breathable film assembly is arranged above the other through hole 201 in connection with the water adding pipe 3. In the utility model, when the prothallus of fern is cultured, the prothallus is put into the dish body 10 by opening the dish cover 2, the dish cover 2 is covered, the water adding pipe 3 is inserted into one of the through holes 201, and the breathable film assembly is arranged above the other through hole 201. In the culture process, the inside of the dish body 10 can be ventilated with the outside through the through hole 201 and the breathable film assembly above the through hole 201. When water needs to be added, the upper blocking piece 4 is removed, the connecting piece 5 is pulled upwards to drive the lower blocking piece 6 to move upwards, the lower blocking piece 6 is inserted into the bottom of the water adding pipe 3 to block the water adding pipe 3, the baffle 601 contacts the bottom end of the water adding pipe 3 to prevent the lower blocking piece 6 from moving upwards, water is added into the water adding pipe 3 until the water flows out of the drain hole 301, and then the upper blocking piece 4 is installed on the water adding pipe 3, the lower blocking piece 6 moves downwards to return to the original position, and the water flows out of the water adding pipe 3, so that the water is added quantitatively. When the dish body 10 and the dish cover 2 are damaged, the water adding assembly and the breathable film assembly can be used with the new dish body 10 and the dish cover 2.

[0023] As a preferred embodiment of the utility model, the following additional technical features can be further provided.

[0024] In the embodiment, the upper blocking piece 4 is threadedly connected with the water adding pipe 3, so that the upper blocking piece 4 can be conveniently installed and removed.

[0025] In the embodiment, the connecting piece 5 is an elastic connecting piece, the upper blocking piece 4 is heavier than the lower blocking piece 6, and the connecting piece 5 is a pull rope or other elastic member, such as a soft hose. After the upper blocking piece 4 is removed, the upper blocking piece 4 is placed outside the water adding pipe 3. Because the upper blocking piece 4 is heavier than the lower blocking piece 6, the lower blocking piece 6 cannot move downwards, and the upper blocking piece 4 does not need to be manually held during the water adding process, so that the operation is convenient.

[0026] In the embodiment, the breathable film assembly comprises a connecting rod 7 and a breathable film 8. The connecting rod 7 is provided with a first air hole 701, and the breathable film 8 is arranged at the first air hole 701. The inside of the dish body 10 is ventilated with the outside through the through hole 201 and the breathable film 8 above the through hole 201, and the connecting rod 7 is connected with the water adding pipe 3. Specifically, the breathable film 8 is a water draining breathable film.

[0027] In the embodiment, the first air hole 701 is a countersunk hole, and the breathable film assembly further comprises a pressing plate 9. The pressing plate 9 is provided with an annular groove 901 and a second air hole 902, and the pressing plate 9 is detachably connected with the first air hole 701 on the connecting rod 7. When the breathable film 8 needs to be replaced, the pressing plate 9 is removed, and the breathable film 8 can be replaced. Specifically, the outer wall of the annular groove 901 is threadedly connected with the inner wall of the upper end of the first air hole 701.

[0028] It is to be understood that the terms "including", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. An element proceeded by "comprises a... " does not, without more constraints, exclude the existence of additional identical elements in the process, method, article, or apparatus that comprises the element.

[0029] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and alterations can be made therein without departing from the spirit and scope of the application, which is defined by the appended claims and their equivalents.

Claims

1. A petri dish for protocorms comprising a dish body (10) and a dish cover (2) which is detachably arranged on the dish body (10), characterized in that The dish cover (2) is provided with two through holes (201) at intervals; further comprising: The water adding assembly comprises a water adding pipe (3), an upper blocking piece (4), a connecting piece (5) and a lower blocking piece (6), the water adding pipe (3) is provided with a plurality of drainage holes (301), the water adding pipe (3) is detachably connected with one of the through holes (201), the upper blocking piece (4) is connected with the lower blocking piece (6) through the connecting piece (5), the lower blocking piece (6) is provided with a baffle (601), the upper blocking piece (4) is detachably connected with the water adding pipe (3), and the lower blocking piece (6) can slide in the water adding pipe (3); The air permeable film assembly is arranged above the other through hole (201) and connected with the water adding pipe (3).

2. A prothallus culture dish according to claim 1, wherein: The upper blocking piece (4) is threadedly connected with the water adding pipe (3).

3. The prothallus culture dish according to claim 1, wherein: The connecting piece (5) is an elastic connecting piece, and the upper blocking piece (4) is heavier than the lower blocking piece (6).

4. The prothallus culture dish according to claim 1, wherein: The air permeable film assembly comprises a connecting rod (7) and an air permeable film (8), the connecting rod (7) is provided with a first air hole (701), and the air permeable film (8) is arranged at the first air hole (701).

5. A prothallus culture dish according to claim 4, wherein: The first air hole (701) is a countersunk hole; the air permeable film assembly further comprises a pressing plate (9), the pressing plate (9) is provided with an annular groove (901) and a second air hole (902), and the pressing plate (9) is detachably connected with the first air hole (701) on the connecting rod (7). The first air hole (701) is a countersunk hole; the air permeable film assembly further comprises a pressing plate (9), the pressing plate (9) is provided with an annular groove (901) and a second air hole (902), and the pressing plate (9) is detachably connected with the first air hole (701) on the connecting rod (7).