Cell culture bag

Abstract

The utility model discloses a cell culture bag, which belongs to the technical field of cell culture, and is characterized in that the cell culture bag comprises a culture bag body with a rectangular bag surface, and a liquid taking pipe and a liquid supplementing pipe which are arranged on the culture bag body, and the liquid taking pipe and the liquid supplementing pipe are arranged at the same end of the culture bag body; sliding rails which extend along the edge of the culture bag body and are parallel to each other are arranged on the two sides, adjacent to the liquid supplementing pipe, of the culture bag body, and the culture bag body is sleeved with a separation sealing piece which moves in the extending direction of the two rails and is used for clamping and separating the interior of the culture bag body into two spaces; the volume of the cell culture bag can be changed according to the requirements of cultured cells, the problems of pollution and inaccurate culture volume control caused by the fact that a container needs to be replaced in the cell culture proliferation process are effectively solved, and the effects of being easy and convenient to operate and high in efficiency are achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell culture, more particularly, it relates to a cell culture bag. BACKGROUND

[0002] In biomedical research, cell suspension is generally loaded into a culture container to achieve sterile culture, expansion and storage of cells, and cell culture work has been widely applied in various fields such as biology, medicine, new drug research and development.

[0003] Among various containers, the culture bag is widely welcomed due to its good air permeability and high cell proliferation efficiency. During the cell culture experiment, since a certain cell density needs to be maintained during the cell culture process, an appropriate container needs to be selected for culture, otherwise the low cell density will affect the growth, but when the cells proliferate to a certain density, too high cell density will cause the cells to stop growing, even aging and apoptosis. However, the existing cell culture bag is generally set with fixed specifications, and the culture volume cannot be adjusted, and the industry generally adopts the method of transferring from a small-capacity cell culture bottle to a large-capacity cell culture bottle, and then to a large cell culture bag for culture, which is quite cumbersome and easy to cause cell contamination. In order to take advantage of the good air permeability and reduce the risk of cell culture contamination, improve the culture efficiency.

[0004] Therefore, the present application provides a cell culture bag with variable volume to solve the above problems. CONTENT OF THE INVENTION

[0005] In view of the deficiencies in the prior art, the purpose of the present application is to provide a cell culture bag, which has the advantages of being able to change the volume of the cell culture bag according to the needs of cell culture, effectively solving the problem of contamination caused by the need to replace containers during cell culture and proliferation, and the problem of inaccurate control of culture volume, and being simple and efficient to operate.

[0006] To achieve the above purpose, the present application provides the following technical scheme: a cell culture bag, comprising a culture bag body with a rectangular bag surface, a liquid taking pipe and a liquid supplementing pipe arranged thereon, the liquid taking pipe and the liquid supplementing pipe being arranged at the same end of the culture bag body, the culture bag body being provided with two sliding rails extending along the edge of the culture bag body and parallel to each other on the two sides adjacent to the liquid supplementing pipe, a separation sealing piece being sleeved on the culture bag body and moving along the extension direction of the two rails and used for clamping and separating the inside of the culture bag body into two spaces, and the two sliding rails are matched with the separation sealing piece.

[0007] By adopting the technical scheme, when the culture bag body is needed to be used for cell culture, the culture bag body volume can be selected according to the required cell culture density, the separation sealing piece is first slid along the extension direction of the two sliding rails towards the liquid supplementing pipe, when the culture bag body volume between the liquid supplementing pipe and the separation sealing piece is the suitable volume, the culture bag body is clamped and separated into two closed spaces by the separation sealing piece, one of the closed spaces has the culture bag body volume between the liquid supplementing pipe and the separation sealing piece, and the other closed space has the culture bag body volume between the separation sealing piece and the end of the culture bag body far from the liquid supplementing pipe; when the cell culture is proliferated to a certain number and the culture bag body volume between the liquid supplementing pipe and the separation sealing piece needs to be increased, the separation sealing piece is opened, the separation sealing piece is moved along the end far from the liquid supplementing pipe, and when the separation sealing piece is moved to a proper distance, the culture bag body is clamped and separated into two closed spaces again by the separation sealing piece, at this time, the culture bag body volume between the liquid supplementing pipe and the separation sealing piece is increased, and the required culture bag body volume value is formed.

[0008] In summary, the space in the culture bag body can be separated into two closed spaces by the sliding rails and the separation sealing piece on the culture bag body, and by moving the distance between the separation sealing piece and the liquid taking pipe, the culture bag body volume between the liquid supplementing pipe and the separation sealing piece can be expanded or reduced, so that the culture bag volume can be changed according to the cell culture requirement, effectively solving the problem that the cell culture proliferation process needs to replace the container, causing pollution and inaccurate culture volume control, and the operation is simple and efficient.

[0009] The utility model further sets up: separation sealing piece is mouth character type elastic piece, separation sealing piece includes respectively with corresponding sliding rail sliding cooperation and both mutually symmetrical setting's support seat, with two support seat is connected and is located in either bag face one side's elastic card slot board, and with two support seat is connected and is located in another face bag face one side's elastic card strip board, elastic card slot board and elastic card strip board card joint cooperation after culture bag body inside is clamped and separated into two spaces.

[0010] By adopting the technical scheme, when the culture bag body is needed to be used for cell culture, the culture bag body volume can be selected according to the required cell culture density, the separation sealing piece is first slid along the extension direction of the two sliding rails towards the liquid supplementing pipe, when the culture bag body volume between the liquid supplementing pipe and the separation sealing piece is the suitable volume, the culture bag body is clamped and separated into two closed spaces by the separation sealing piece, one of the closed spaces has the culture bag body volume between the liquid supplementing pipe and the separation sealing piece, and the other closed space has the culture bag body volume between the separation sealing piece and the end of the culture bag body far from the liquid supplementing pipe; when the cell culture is proliferated to a certain number and the culture bag body volume between the liquid supplementing pipe and the separation sealing piece needs to be increased, the separation sealing piece is opened, the separation sealing piece is moved along the end far from the liquid supplementing pipe, and when the separation sealing piece is moved to a proper distance, the culture bag body is clamped and separated into two closed spaces again by the separation sealing piece, at this time, the culture bag body volume between the liquid supplementing pipe and the separation sealing piece is increased, and the required culture bag body volume value is formed.

[0011] The utility model further sets up: the elastic card strip board includes with two first elastic plate of support base connection, and the groove type strip of being set up on the first elastic plate and being close to the bag face of cultivation bag body, the groove type strip length is greater than the distance length between the two sliding groove corresponding cultivation bag body inside, the groove type strip is provided with the clamping groove extending along its length direction to cultivation bag body one side, the elastic card strip board includes with two second elastic plate of support base connection, and the clamping strip of being set up on the second elastic plate and being close to the bag face of cultivation bag body, the clamping strip is equal in length with the clamping groove, the clamping strip and the clamping groove are mutually clamped and clamp tightly close cultivation bag body two sides.

[0012] By adopting the above technical scheme, when the cultivation bag body inside is clamped and separated into two spaces by the separation sealing piece, the clamping strip on the second elastic plate is embedded in the clamping groove on the first elastic plate, and the clamping strip and the clamping groove are firmly clamped, thereby effectively avoiding the two bag surfaces from being separated from the clamping groove.

[0013] The utility model further sets up: the clamping groove cross section is arc-shaped, the clamping strip is embedded in the clamping groove, and the first elastic plate and the second elastic plate are respectively provided with a force applying strip at the end away from the cultivation bag body.

[0014] By adopting the above technical scheme, the clamping groove cross section is arc-shaped and the clamping strip is embedded in the clamping groove, which facilitates the installation of the clamping strip in the clamping groove; the force applying strip facilitates the application of force to the first elastic plate and the second elastic plate, thereby facilitating the embedding and separation of the clamping groove and the clamping strip.

[0015] The utility model further sets up: the sliding rail cross section is convex-shaped, and the sliding groove is provided on the two support bases.

[0016] By adopting the above technical scheme, the sliding rail cross section is convex-shaped and the sliding groove is provided, which facilitates the limiting of the separation sealing piece and makes the movement of the separation sealing piece more stable.

[0017] The utility model further sets up: the separation sealing piece is made of plastic material, and the cultivation bag body is made of EVA plastic.

[0018] The utility model further sets up: the cultivation bag body is provided with a capacity scale mark on the side edge of the sliding rail.

[0019] By adopting the above technical scheme, the capacity scale mark facilitates the quick understanding of the cultivation bag body volume between the liquid supplement pipe and the separation sealing piece when the separation sealing piece moves to the relative position.

[0020] In summary, the utility model has the following advantages:

[0021] 1、Through the sliding rail on the culture bag body and the separation sealing piece, the internal space of the culture bag body can be separated into two closed spaces, and by moving the distance between the separation sealing piece and the liquid taking pipe, the culture bag body volume between the liquid supplementing pipe and the separation sealing piece can be expanded or reduced, so that the culture bag volume can be changed according to the needs of cell culture, effectively solving the problem of pollution caused by the need to replace the container during cell culture and proliferation, and the operation is simple and efficient.

[0022] 2、When the culture bag body needs to be clamped and separated into two spaces by the separation sealing piece, the elastic clamping groove plate and the elastic clamping strip plate can be simultaneously subjected to opposite forces, so that the elastic clamping groove plate and the elastic clamping strip plate move towards each other and clamp the two bag surfaces of the culture bag body therebetween, and the two bag surfaces between the elastic clamping groove plate and the elastic clamping strip plate are tightly attached, so that the culture bag body is clamped and separated into two spaces.

[0023] 3、By setting the capacity scale, when the separation sealing piece moves to the relative position, the culture bag body volume between the liquid supplementing pipe and the separation sealing piece can be quickly understood. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is the overall structure schematic diagram of the embodiment;

[0025] Figure 2 is the cross-sectional structure schematic diagram of the embodiment;

[0026] Figure 3 is the structure schematic diagram of the separation sealing piece of the embodiment;

[0027] Figure 4 is the structure cross-sectional view of the clamping of the elastic clamping groove plate and the elastic clamping strip plate of the embodiment.

[0028] Explanation of reference signs: 1, culture bag body; 2, liquid taking pipe; 3, liquid supplementing pipe; 4, sliding rail; 5, capacity scale; 6, support seat; 7, elastic clamping groove plate; 71, first elastic plate; 72, groove type strip; 73, clamping groove; 8, elastic clamping strip plate; 81, second elastic plate; 82, clamping strip; 9, force applying strip; 10, sliding groove. DETAILED DESCRIPTION

[0029] The utility model will be further described in detail below in combination with the drawings.

[0030] A kind of cell culture bag, such as Figure 1 、 2As shown, the culture bag body 1 has a rectangular bag surface, a liquid taking pipe 2 and a liquid supplementing pipe 3 are mounted on the culture bag body 1, the liquid taking pipe 2 and the liquid supplementing pipe 3 are mounted on the same end of the culture bag body 1, the culture bag body 1 is integrally formed with two sliding rails 4 which extend along the edges of the culture bag body 1 and are parallel to each other on the two sides adjacent to the liquid supplementing pipe 3, a separation sealing member which moves along the extension direction of the two rails and is used for clamping and separating the inside of the culture bag body 1 into two spaces is sleeved on the culture bag body 1, the two sliding rails 4 are matched with the separation sealing member, the separation sealing member is made of plastic, and the culture bag body 1 is made of EVA plastic.

[0031] Further, capacity scale marks 5 which are consistent with the extension direction of the sliding rails 4 are engraved on the edge of the culture bag body 1 on one side of the sliding rails 4, which helps to quickly understand the volume of the culture bag body 1 between the liquid supplementing pipe 3 and the separation sealing member when the separation sealing member moves to the relative position.

[0032] As shown in Figure 1 , 2 , the cross section of the sliding rail 4 is arranged in a convex shape, and the two support seats 6 are respectively provided with a sliding groove 10 which is matched with the corresponding sliding rail 4. The cross section of the sliding rail 4 is arranged in a convex shape and the sliding groove 10 is arranged, which helps to limit the separation sealing member and make the movement of the separation sealing member more stable.

[0033] As shown in Figure 3 , 4 , the separation sealing member is an elastic member which is arranged in a mouth shape and is integrally formed, the separation sealing member includes support seats 6 which are respectively matched with the corresponding sliding rails 4 and are arranged symmetrically with each other, an elastic slot plate 7 which is connected with the two support seats 6 and is located on one side of any bag surface, and an elastic strip plate 8 which is connected with the two support seats 6 and is located on the other side of the bag surface, the elastic slot plate 7 and the elastic strip plate 8 are clamped and matched to clamp and separate the inside of the culture bag body 1 into two spaces.

[0034] When it is needed to clamp and separate the inside of the culture bag body 1 into two spaces through the separation sealing member, the elastic slot plate 7 and the elastic strip plate 8 can be simultaneously subjected to opposite forces, so that the elastic slot plate 7 and the elastic strip plate 8 move towards each other and clamp the two bag surfaces of the culture bag body 1 therebetween, and the two bag surfaces located between the elastic slot plate 7 and the elastic strip plate 8 are tightly attached, so that the inside of the culture bag body 1 is clamped and separated into two spaces.

[0035] As shown in Figure 3 , 4As shown, the elastic clamping strip plate 8 comprises a first elastic plate 71 connected with the two support seats 6, and a groove-shaped strip 72 fixed on the first elastic plate 71 and close to the bag surface of the culture bag body 1, the length of the groove-shaped strip 72 is greater than the distance between the two sliding grooves 10 corresponding to the inside of the culture bag body 1, and the groove-shaped strip 72 is provided with a clamping groove 73 extending along the length direction thereof towards one side of the culture bag body 1; the elastic clamping strip plate 8 comprises a second elastic plate 81 connected with the two support seats 6, and a clamping strip 82 fixed on the second elastic plate 81 and close to the bag surface of the culture bag body 1, the length of the clamping strip 82 is equal to that of the clamping groove 73, and the clamping strip 82 and the clamping groove 73 are clamped with each other and clamp and close the two bag surfaces of the culture bag body 1. When the inside of the culture bag body 1 is clamped and separated into two spaces by the separation sealing element, the clamping strip 82 on the second elastic plate 81 is embedded in the clamping groove 73 on the first elastic plate 71, and the clamping strip 82 and the clamping groove 73 are firmly clamped, thereby effectively avoiding the two bag surfaces from being separated from the clamping groove 73.

[0036] Further, as shown in the drawings, Figure 4 The cross section of the clamping groove 73 is arc-shaped, the clamping strip 82 is embedded in the clamping groove 73, and the first elastic plate 71 and the second elastic plate 81 are respectively provided with a force applying strip 9 at the end away from the culture bag body 1. The cross section of the clamping groove 73 is arc-shaped and the clamping strip 82 is embedded in the clamping groove 73, which facilitates the installation of the clamping strip 82 in the clamping groove 73; the force applying strip 9 facilitates the application of force to the first elastic plate 71 and the second elastic plate 81, thereby facilitating the embedding and separation of the clamping groove 73 and the clamping strip 82.

[0037] The working process and beneficial effects of the utility model are as follows: when the culture bag body 1 is needed to be used for cell culture, the volume of the culture bag body 1 can be selected according to the required cell density, the separation sealing element is first slid along the extension direction of the two sliding rails 4 towards the liquid supplementing pipe 3, when the volume of the culture bag body 1 between the liquid supplementing pipe 3 and the separation sealing element is the suitable volume, the inside of the culture bag body 1 is clamped and separated into two airtight spaces by the separation sealing element, one of the airtight spaces has the volume of the culture bag body 1 between the liquid supplementing pipe 3 and the separation sealing element, and the other airtight space has the volume of the culture bag body 1 between the separation sealing element and the end away from the liquid supplementing pipe 3; when the cell culture and proliferation reach a certain amount and the volume of the culture bag body 1 between the liquid supplementing pipe 3 and the separation sealing element needs to be increased, the separation sealing element is opened, the separation sealing element is moved along the end away from the liquid supplementing pipe 3, and when the separation sealing element is moved to a proper distance, the inside of the culture bag body 1 is again clamped and separated into two airtight spaces by the separation sealing element, at this time, the volume of the culture bag body 1 between the liquid supplementing pipe 3 and the separation sealing element is increased, and the required volume value of the culture bag body 1 is formed.

[0038] In summary, the inner space of the culture bag body 1 can be divided into two closed spaces by the sliding rail 4 on the culture bag body 1 and the separation sealing piece, and by changing the distance between the separation sealing piece and the liquid taking pipe 2, the volume of the culture bag body 1 between the liquid supplementing pipe 3 and the separation sealing piece can be enlarged or reduced, so that the volume of the cell culture bag can be changed according to the needs of cell culture, effectively solving the problems of pollution caused by the need to replace the container during the cell culture and proliferation process and inaccurate control of the culture volume, and achieving the purposes of simple operation and high efficiency.

[0039] The above merely describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. within the design concept of the present application shall be included in the protection scope of the present application.

Claims

1. A cell culture bag comprising a bag body (1) with a rectangular bag face, a liquid withdrawal tube (2) and a liquid supplement tube (3) arranged thereon, characterized in that: The liquid taking pipe (2) and the liquid supplementing pipe (3) are arranged at the same end of the culture bag body (1), the culture bag body (1) is provided with two sliding rails (4) which extend along the edges of the culture bag body (1) and are parallel to each other on the two sides adjacent to the liquid supplementing pipe (3), a separation sealing piece which moves along the extension direction of the two rails and is used for clamping and separating the inside of the culture bag body (1) into two spaces is sleeved on the culture bag body (1), and the two sliding rails (4) are matched with the separation sealing piece.

2. The cell culture bag of claim 1, wherein: The separation sealing piece is a mouth-shaped elastic piece, the separation sealing piece comprises support seats (6) which are matched with the corresponding sliding rails (4) and are symmetrically arranged, an elastic clamping groove plate (7) which is connected with the two support seats (6) and is arranged on one side of any bag surface, and an elastic clamping strip plate (8) which is connected with the two support seats (6) and is arranged on the other side of the bag surface, and the elastic clamping groove plate (7) and the elastic clamping strip plate (8) are clamped and matched to clamp and separate the inside of the culture bag body (1) into two spaces.

3. The cell culture bag of claim 2, wherein: The elastic clamping strip plate (8) comprises a first elastic plate (71) which is connected with the two support seats (6), and a groove-shaped strip (72) which is arranged on the first elastic plate (71) and is close to the bag surface of the culture bag body (1), the length of the groove-shaped strip (72) is greater than the length of the distance between the inside of the culture bag body (1) corresponding to the two sliding grooves (10), and the groove-shaped strip (72) is provided with a clamping groove (73) which extends along the length direction of the groove-shaped strip (72) and is arranged on one side of the culture bag body (1); the elastic clamping strip plate (8) comprises a second elastic plate (81) which is connected with the two support seats (6), and a clamping strip (82) which is arranged on the second elastic plate (81) and is close to the bag surface of the culture bag body (1), the length of the clamping strip (82) is equal to that of the clamping groove (73), and the clamping strip (82) and the clamping groove (73) are clamped with each other to clamp and close the two surfaces of the culture bag body (1).

4. The cell culture bag of claim 3, wherein: The cross section of the clamping groove (73) is arranged in an arc shape, the clamping strip (82) is embedded in the clamping groove (73), and the ends of the first elastic plate (71) and the second elastic plate (81) which are away from the culture bag body (1) are respectively provided with force applying strips (9) for applying force.

5. The cell culture bag of claim 2, wherein: The cross section of the sliding rail (4) is arranged in a convex shape, and the sliding grooves (10) which are matched with the corresponding sliding rails (4) are respectively arranged on the two support seats (6).

6. The cell culture bag of claim 1, wherein: The separation sealing piece is made of plastic, and the culture bag body (1) is made of EVA plastic.

7. The cell culture bag of claim 1, wherein: The capacity scale marks (5) which are consistent with the extension direction of the sliding rail (4) are arranged on the edge of one side of the sliding rail (4) of the culture bag body (1).

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