Sample pool test paper soaking device for detecting organophosphorus pesticide
By designing a sample pool test strip immersion device and adopting the principle of UCNPs and antigen-antibody binding, the problem of long detection time and poor specificity of traditional OPs test strips is solved, realizing rapid, specific and accurate OPs detection, which is suitable for simple screening of on-site samples and subsequent precise analysis.
Patent Information
- Application Number
- CN202520133116.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-21
- Publication Date
- 2026-02-06
- Estimated Expiration
- 2035-01-21
AI Technical Summary
Existing OPs test strips absorb the dripped sample solution through capillary action, resulting in a long reaction time and a lack of specific detection function.
A sample cell test strip immersion device for detecting organophosphorus pesticides was designed. UCNPs were used as fluorescent donors, and combined with the principle of antigen-antibody specific binding, rare earth-doped upconversion nanomaterials and graphene oxide were used as fluorescent acceptors. The test strips were rapidly immersed in the immersion tank, and a multi-T line design was introduced to achieve specific detection.
It achieves rapid, specific, and accurate detection of ops, enabling simple on-site screening followed by precise analysis. The detection time is short and the results are accurate, while also being portable and efficient.
Smart Images
Figure CN223883579U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of pesticide detection, especially a sample pool test paper soaking device for detecting organophosphorus pesticides. BACKGROUND
[0002] Organophosphorus pesticides (OPs) are compounds containing phosphorus elements, which are mainly used for preventing and treating plant diseases, insect pests and weed, and are widely used in agricultural, forestry and animal husbandry production in countries around the world, and have played an important role in increasing agricultural yield. The excessive use of OPs in large quantities will lead to excessive residues in the environment and crops, causing environmental pollution of soil, air and water. The toxicity mechanism of OPs is to inhibit the activity of acetylcholinesterase (AChE) in nerve tissue and other target organs, and acetylcholine (ACh) cannot be decomposed, causing excessive stimulation of muscarinic and nicotinic receptors, and then causing clinical symptoms such as excessive sweating, salivation, bronchial spasm, bronchial edema, bradycardia, hypotension and decreased consciousness. Even low levels of OPs can cause great harm to human health, and can cause kidney damage, Parkinson's disease, endocrine disorders, memory loss and proximal muscle weakness. Due to the harm of OPs to human health, it is of great significance to qualitatively and quantitatively analyze and detect trace and trace OPs residues in the environment and agricultural products.
[0003] The commonly used methods for detecting OPs include chromatography, mass spectrometry, high-performance liquid chromatography-tandem mass spectrometry, electrochemical method, enzyme-linked immunosorbent assay and enzyme inhibition method. Chromatography, mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry are traditional instrument detection methods, which have high accuracy but complex sample pretreatment method, high detection cost and cannot realize rapid detection; although the electrochemical method has low detection cost and can realize rapid detection, it has poor repeatability, which affects the accuracy of the detection results; the enzyme inhibition method has good accuracy, but when applied to the detection of OPs, it does not have specificity, OPs have enzyme inhibition, this method can only detect the total amount of OPs, and cannot specifically detect a single specific pesticide. Therefore, it is of great significance to establish a sensitive, rapid, accurate and specific detection method for OPs residues.
[0004] The current OPs test strip product utilizes the enzyme inhibition of OPs on acetylcholinesterase, and OPs have enzyme inhibition, this method can only qualitatively detect the type of OPs, and does not have the function of specific detection. And the traditional paper-based OPs test strip utilizes capillary action to absorb the drop of the sample solution to be detected, and the action time is long. UTILITY MODEL CONTENT
[0005] In order to solve the problem of long action time of the existing OPs test paper strip using capillary action to absorb the drop of the sample solution to be measured in the background art, the utility model provides a sample pool test paper soaking device for detecting organic phosphorus pesticide.
[0006] The technical scheme of the utility model is: a sample pool test paper soaking device for detecting organic phosphorus pesticide, including the infiltration groove and test paper strip of upper opening, the infiltration groove is used to contain the OPs solution to be detected;
[0007] The right side plate of the infiltration groove is provided with the first through hole that is left and right through, the left side plate of the infiltration groove is provided with the second through hole that is left and right through, the first through hole and the second through hole are left and right corresponding;
[0008] The third deflection roller and the fourth deflection roller that are left and right spaced are rotationally connected between the front and rear side plates of the infiltration groove, and the top of the third deflection roller and the fourth deflection roller are all lower than the first through hole and the second through hole.
[0009] One end of the test paper strip passes through the first through hole, passes around the bottom of the third deflection roller and the fourth deflection roller and passes out from the second through hole;
[0010] The test paper strip is provided with a detection area, and the detection area is provided with a T line area, a C line area, a PH test paper segment and a blank segment located at the left and right two outer sides of the T line area and the PH test paper segment, which are left and right spaced, the T line area, the C line area and the PH test paper segment are located between the third deflection roller and the fourth deflection roller, and the blank segments at the left and right two outer sides are respectively arranged in the second through hole and the first through hole.
[0011] The T line area sequentially includes a backing card, a sample pad, a UCNPs-mAbs layer and a GO-Ag layer from bottom to top, the UCNPs-mAbs layer includes a NaYF4:Yb,Er@NaYF4 layer as a fluorescent donor and OPs monoclonal antibody modified on the surface of the NaYF4:Yb,Er@NaYF4 layer, and the GO-Ag layer includes a GO layer as a fluorescent acceptor and OPs antigen coupled with the surface of the GO layer.
[0012] The C line area sequentially includes a backing card, a sample pad and a UCNPs layer from bottom to top, and the UCNPs layer includes a NaYF4:Yb,Er@NaYF4 layer.
[0013] Preferably, the test paper strip is provided with a plurality of detection areas connected in left and right succession, and the detection area includes a T line area, a C line area, a PH test paper segment and a blank segment which are left and right spaced.
[0014] The two blank segments of the detection area located at the left end are respectively arranged in the second through hole and the first through hole.
[0015] Preferably, the detection area is provided with a plurality of T line areas, one C line area and one PH test paper segment which are left and right spaced.
[0016] The UCNPs-mAbs layer in each T-line region is modified with different kinds of OPs monoclonal antibodies, and the GO-Ag layer in each T-line region is modified with OPs antigens capable of specifically binding to the OPs monoclonal antibodies in the region.
[0017] Preferably, a winding device is fixedly arranged on the right side of the infiltration groove, and the right end of the test strip is wound on the winding device, and the winding device is located below the first through hole.
[0018] A first positioning device for clamping and fixing the test strip is arranged on the right side plate of the infiltration groove, and the first positioning device is located between the first through hole and the winding device.
[0019] A second positioning device for clamping and fixing the test strip is arranged on the left side plate of the infiltration groove, and the second positioning device is located below the second through hole.
[0020] Preferably, the first positioning device comprises a first magnet block embedded in the right side plate of the infiltration groove, a detachable second magnet block is adsorbed to the right side of the first magnet block, a first handle is arranged on the second magnet block, and the test strip is arranged between the first magnet block and the second magnet block.
[0021] The second positioning device comprises a third magnet block embedded in the left side plate of the infiltration groove, a detachable fourth magnet block is adsorbed to the left side of the third magnet block, a second handle is arranged on the fourth magnet block, and the test strip is arranged between the third magnet block and the fourth magnet block.
[0022] Preferably, the front and rear side plates of the infiltration groove are rotatably connected with a second steering roller and a fifth steering roller arranged at intervals left and right.
[0023] The second steering roller is located above and right of the third steering roller, and the upper end of the second steering roller is flush with the bottom of the first through hole.
[0024] The fifth steering roller is located above and left of the fourth steering roller, and the upper end of the fifth steering roller is flush with the bottom of the second through hole.
[0025] The left end of the test strip sequentially passes through the first through hole, the second steering roller, the third steering roller, the fourth steering roller, the fifth steering roller, and the second through hole from right to left.
[0026] Preferably, a second mounting bracket is fixedly arranged on the right side plate of the infiltration groove, a first steering roller extending in the front-back direction is rotatably arranged on the second mounting bracket, the first steering roller is located between the first positioning device and the first through hole, and the top of the first steering roller is flush with the bottom of the first through hole.
[0027] The left end of the test strip sequentially passes through the first steering roller, the first through hole, the second steering roller, the third steering roller, the fourth steering roller, the fifth steering roller, and the second through hole from right to left.
[0028] Preferably, a third through hole is formed in the left side plate of the infiltration tank, the third through hole is in communication with the second through hole at the bottom and with the outside at the top, and a cutting device capable of moving up and down is arranged in the third through hole, and the upper end of the cutting device extends to the outside of the third through hole.
[0029] A backing plate is fixedly arranged at the bottom of the second through hole, and the cutting device and the backing plate are matched to cut the test paper strip.
[0030] Preferably, a limiting block is fixedly arranged on the inner side wall of the third through hole, and a plurality of movable holes are formed in the limiting block in a front-rear interval and in a up-down penetration.
[0031] A cutter extending in the front-rear direction is arranged below the limiting block, and the front-rear length of the cutter is greater than the front-rear width of the test paper strip.
[0032] A plurality of first connecting rods are fixedly arranged on the top of the cutter in a front-rear interval, a sliding block is fixedly arranged on the first connecting rod, the left and right side surfaces of the sliding block are in sliding contact with the left and right inner walls of the third through hole, a return spring is sleeved on the first connecting rod, the lower end of the return spring abuts against the limiting block, the upper end of the return spring abuts against the bottom of the sliding block, the upper end of the first connecting rod penetrates out of the third through hole, and a pressing plate is fixedly connected to the upper end of the first connecting rod.
[0033] When the return spring is in the initial state, the lower end of the cutter is located in the third through hole.
[0034] Preferably, a timer is fixedly arranged on the infiltration tank.
[0035] The test paper strip uses UCNPs (rare earth doped upconversion nanomaterial) as a fluorescent donor, and the material has the advantages of long luminescence lifetime, stable chemical properties, low toxicity, surface modification, small light damage to biological samples, no spontaneous fluorescence interference and the like, so that the test paper strip stored according to the regulation has a long shelf life. The fluorescent performance is stable and basically not affected by time.
[0036] (2) Compared with large instruments, the device has the advantages of portability and high efficiency of detection. In the detection of field samples, the test paper strip can be used for qualitative analysis of actual samples, simple and accurate screening of a large number of samples in advance, and convenient further accurate analysis and detection of screened samples.
[0037] (3) After the test paper strip is soaked in the OPs solution to be detected, a portable fluorescence enzyme marker with a 980nm excitation light or a Hitachi fluorescence spectrophotometer f-4600 with a solid sample fixing frame can be selected for analysis and detection according to the actual situation.
[0038] (4) The pH test paper section on the test paper strip can show the pH of the sample to be tested, determine whether the test paper strip test condition is optimal, and determine whether the detection result is relatively accurate.
[0039] (5) The T line area of the test paper strip introduces specific antigen-antibody binding principles, and adopts a multi-T line type test paper strip design, so that different OPs antigen-antibody pairs can be selected for specific detection of target OPs according to actual conditions, and compared with the traditional enzyme inhibition OPs test paper strip, the OPs detection specificity of the test paper strip can be further improved, and specific types of OPs can be detected, and different OPs antigen-antibody pairs can be selected for specific detection of target OPs according to actual conditions.
[0040] (6) The test paper strip detects the specific numerical value of the fluorescence signal, and compared with the traditional colorimetric method, the detection result is more accurate and sensitive.
[0041] (7) The test paper strip is soaked in the soaking groove, and compared with the traditional capillary paper base test paper strip, the soaking is faster, more direct, and the contact reaction area is larger, so that on-site rapid specific classification qualitative and quantitative detection of OPs is realized, and the detection time is shorter. BRIEF DESCRIPTION OF DRAWINGS
[0042] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the drawings needed in the embodiment or the prior art description will be briefly introduced below, and obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to these drawings without creative labor.
[0043] Figure 1 It is a schematic view of the main body structure of the embodiment 1.
[0044] Figure 2 It is a schematic view of the main body structure of the embodiment 1. Figure 1
[0045] Figure 3 It is an enlarged view of the structure at A in the embodiment 1. Figure 1
[0046] Figure 4 It is an enlarged view of the structure at B in the embodiment 1. Figure 1
[0047] In the figure, 1, infiltration tank, 101, first through hole, 102, second through hole, 103, backing plate, 104, third through hole, 105, limiting block, 2, test strip, 201, blank section, 202, T line area, 203, C line area, 204, PH test paper section, 3, solution to be detected OP, 4, reel device, 5, first magnet block, 6, second magnet block, 7, first handle, 8, first deflection roller, 9, second deflection roller, 10, third deflection roller, 11, fourth deflection roller, 12, fifth deflection roller, 13, pressing plate, 14, first connecting rod, 15, return spring, 16, cutter, 17, sixth deflection roller, 18, third magnet block, 19, fourth magnet block, 20, second handle, 21, timer. DETAILED DESCRIPTION
[0048] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.
[0049] Embodiment 1: a sample pool test paper immersion device for detecting organophosphorus pesticides, as shown in the figure, comprising an upper opening infiltration tank 1 and a test strip 2, the infiltration tank 1 is used to contain the solution to be detected OP 3. Figure 1 As shown in the figure, the test strip 2 is provided with a plurality of left and right continuous connection detection areas, a plurality of left and right interval T line areas 202, a C line area 203 and a PH test paper section 204 are arranged in the detection area, and the left and right two sides of the T line area 202 and the PH test paper section 204 are provided with a blank section 201.
[0050] As shown in the figure, the test strip 2 is provided with a plurality of left and right continuous connection detection areas, a plurality of left and right interval T line areas 202, a C line area 203 and a PH test paper section 204 are arranged in the detection area, and the left and right two sides of the T line area 202 and the PH test paper section 204 are provided with a blank section 201. Figure 2
[0051] The UCNPs-mAbs layer in each T line area 202 is modified with different types of OP monoclonal antibodies, and the GO-Ag layer in each T line area 202 is modified with OP antigens capable of specifically combining with the OP monoclonal antibodies in the area. So that the test strip 2 can specifically detect different types of OPs.
[0052] As shown in the figure, the right side plate of the infiltration tank 1 is provided with a left and right through first through hole 101, and the left side plate of the infiltration tank 1 is provided with a left and right through second through hole 102, and the first through hole 101 and the second through hole 102 are left and right corresponding. Figure 3
[0053] The right side of the infiltration tank 1 is fixedly provided with a winding drum device 4, which is located below the first through hole 101. The winding drum device 4 in the embodiment includes a first mounting frame fixedly provided on the right side plate of the infiltration tank 1 and a winding drum rotatably provided on the first mounting frame, and the right end of the test strip 2 is wound on the winding drum.
[0054] In order to fix the region of the test strip 2 between the winding drum and the first through hole 101, the right side plate of the infiltration tank 1 is provided with a first positioning device for clamping and fixing the test strip 2, which is located between the first through hole 101 and the winding drum device 4. Specifically, as shown in Figure 3 The first positioning device includes a first magnet block 5 embedded on the right side plate of the infiltration tank 1, a detachable second magnet block 6 is adsorbed on the right side of the first magnet block 5, a first handle 7 is provided on the second magnet block 6, and the test strip 2 is provided between the first magnet block 5 and the second magnet block 6.
[0055] In order to avoid the test strip 2 from being seriously abraded with the right end of the first through hole 101 when entering the first through hole 101, as shown in Figure 3 The right side plate of the infiltration tank 1 is fixedly provided with a second mounting frame, and a first deflection roller 8 extending in the front-rear direction is rotatably provided on the second mounting frame, the first deflection roller 8 is located between the first positioning device and the first through hole 101, and the top of the first deflection roller 8 is flush with the bottom of the first through hole 101.
[0056] The third deflection roller 10 and the fourth deflection roller 11 are rotatably connected between the front and rear side plates of the infiltration tank 1 and are arranged at intervals left and right, and the top of each of the third deflection roller 10 and the fourth deflection roller 11 is lower than the first through hole 101 and the second through hole 102.
[0057] The second deflection roller 9 and the fifth deflection roller 12 are rotatably connected between the front and rear side plates of the infiltration tank 1 and are arranged at intervals left and right, the second deflection roller 9 is located above the right of the third deflection roller 10, the upper end of the second deflection roller 9 is flush with the bottom of the first through hole 101, the fifth deflection roller 12 is located above the left of the fourth deflection roller 11, and the upper end of the fifth deflection roller 12 is flush with the bottom of the second through hole 102.
[0058] In order to facilitate the cutting of the detection region of the left end of the detected test strip 2, a third through hole 104 is formed in the left side plate of the infiltration tank 1, which is vertically through, the bottom of the third through hole 104 is communicated with the second through hole 102, the top of the third through hole 104 is communicated with the outside, a cutting device capable of moving up and down is arranged in the third through hole 104, and the upper end of the cutting device extends to the outside of the third through hole 104; the bottom of the second through hole 102 is fixedly provided with a backing plate 103, and the cutting device and the backing plate 103 cooperate to cut the test strip 2. Specifically, as shown in Figure 4As shown, the inner side wall of the third through hole 104 is fixedly provided with a limiting block 105, a plurality of movable holes are formed in the limiting block 105 and arranged at intervals in front and back and vertically through; the lower portion of the limiting block 105 is provided with a cutter 16 extending along the front and back direction, the front and back length of the cutter 16 is greater than the front and back width of the test strip 2; the top of the cutter 16 is fixedly provided with a plurality of first connecting rods 14 arranged at intervals in front and back, the first connecting rod 14 is fixedly provided with a sliding block, the left and right sides of the sliding block are in sliding contact with the left and right inner walls of the third through hole 104, the first connecting rod 14 is sleeved with a return spring 15, the lower end of the return spring 15 abuts against the limiting block 105, the upper end of the return spring 15 abuts against the bottom of the sliding block, the upper end of the first connecting rod 14 penetrates out of the third through hole 104, and the upper end of the first connecting rod 14 is fixedly connected with a pressing plate 13; when the return spring 15 is in the initial state, the lower end of the cutter 16 is located in the third through hole 104.
[0059] In order to fix the left end of the test strip 2, the left side plate of the infiltration groove 1 is provided with a second positioning device for clamping and fixing the test strip 2, and the second positioning device is located below the second through hole 102. Specifically, as shown in Figure 4 The second positioning device includes a third magnet block 18 embedded in the left side plate of the infiltration groove 1, the left side of the third magnet block 18 is adsorbed with a detachable fourth magnet block 19, the fourth magnet block 19 is provided with a second handle 20, and the test strip 2 is arranged between the third magnet block 18 and the fourth magnet block 19.
[0060] In order to prevent the test strip 2 from being pulled out of the second through hole 102 and causing serious abrasion with the left end of the second through hole 102, as shown in Figure 4 The left side plate of the infiltration groove 1 is fixedly provided with a third mounting bracket, and the third mounting bracket is rotatably provided with a sixth deflection roller 17.
[0061] The left end of the test strip 2 sequentially passes through the first deflection roller 8, the first through hole 101, the second deflection roller 9, the third deflection roller 10, the fourth deflection roller 11, the fifth deflection roller 12, the second through hole 102 and the sixth deflection roller 17 from right to left.
[0062] The T-line area 202, the C-line area 203 and the PH test paper segment 204 of the detection area located at the left end of the test strip 2 are located between the third deflection roller 10 and the fourth deflection roller 11, and the left and right outer blank segments 201 are arranged in the second through hole 102 and the first through hole 101 respectively.
[0063] The T-line area 202 includes, from bottom to top, a backing card, a sample pad, a UCNPs-mAbs layer, and a GO-Ag layer. The UCNPs-mAbs layer includes a NaYF4:Yb,Er@NaYF4 layer as a fluorescent donor and OPs monoclonal antibodies modified on the surface of the NaYF4:Yb,Er@NaYF4 layer. The GO-Ag layer includes a GO layer as a fluorescent acceptor and OPs antigens coupled to the surface of the GO layer.
[0064] The C-line area 203 includes, from bottom to top, a backing card, a sample pad, and a UCNPs layer. The UCNPs layer includes a NaYF4:Yb,Er@NaYF4 layer.
[0065] To facilitate accurate recording of the soaking time of the test strip 2, a timer 21 is fixed on the soaking groove 1, as shown. Figure 1
[0066] Working principle: The test strip 2 of the device selects NaYF4:Yb,Er@NaYF4 rare earth doped upconversion nanomaterial as the fluorescent donor of the T-line area and the C-line area. Because of the core-shell structure, the fluorescence quenching caused by the surface defects of the nanomaterial is reduced, the fluorescence value is increased, and the sensitivity of the fluorescence sensor is further improved. Graphene oxide (GO) is selected as the fluorescent acceptor of the T-line area. By taking advantage of the characteristics of the surface of GO that can be modified with different functional groups and the wide ultraviolet absorption spectrum, the fluorescence emitted by the fluorescent donor is absorbed.
[0067] The traditional enzyme inhibition type organophosphorus pesticide sensor has poor specificity. The device further enhances the specificity of the sensor by applying the specific binding of antigens and antibodies. OPs monoclonal antibodies are modified on the surface of NaYF4:Yb,Er@NaYF4, and OPs antigens are coupled to the surface of GO. Through the specific binding of antigens and antibodies, the distance between the fluorescent donor and the fluorescent acceptor is less than 10 nm, which meets the principle of fluorescence resonance energy transfer. The fluorescence emitted by NaYF4:Yb,Er@NaYF4 is absorbed and quenched by GO. OPs in the system compete with antigens, causing some fluorescent probes to separate from the fluorescent acceptor GO, and the fluorescence of the sensor is restored. Based on this principle, specific qualitative and quantitative detection of OPs in actual samples of agricultural products is realized.
[0068] The PH test paper section 204 can detect the PH acidity and alkalinity of the current solution, determine whether the test conditions of the test strip 2 are optimal, and determine whether the detection result is relatively accurate.
[0069] In use, first, the left end of the test strip 2 is placed in the soaking groove 1, as shown in Figure 1 The test area of the test strip 2 at the left end passes through the first diverting roller 8, the first through hole 101, the second diverting roller 9, the third diverting roller 10, the fourth diverting roller 11, the fifth diverting roller 12, the second through hole 102 and the sixth diverting roller 17 in sequence, so that the T line area 202 and the C line area 203 of the test area at the left end of the test strip 2 are located between the third diverting roller 10 and the fourth diverting roller 11, and the blank sections 201 at the left and right sides pass through the second through hole 102 and the first through hole 101 respectively.
[0070] The second magnet block 7 is adsorbed on the first magnet block 6 to fix the right part of the test strip 2, and the fourth magnet block 19 is adsorbed on the third magnet block 18 to fix the left part of the test strip 2.
[0071] The OPs solution to be detected 3 is poured into the infiltration groove 1, so that the upper surface of the OPs solution to be detected 3 is above the top of the test strip 2 between the third diverting roller 10 and the fourth diverting roller 11.
[0072] The timer 21 is used to record the reaction time, the reaction is incubated at room temperature for 30 min, and the fluorescence value of a specific wave band at the T line and the C line is detected by using a fluorescence signal detection instrument with a 980 nm excitation light source; the fluorescence values of the T line and the C line of a positive result are basically consistent, and both have strong fluorescence, while the fluorescence value of the T line of a negative result is obviously weaker than that of the C line. Since the test strip is a multi-T line device, when the fluorescence value of the corresponding T line is positive, it is proved that the test sample solution contains the corresponding specific OPs.
[0073] It is obvious for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be realized in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be regarded as exemplary and non-limiting, the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims.
Claims
1. A sample cell test paper dipper for detecting organophosphorus pesticides, characterized by: The device comprises an upper opening infiltration groove (1) and a test strip (2), the infiltration groove (1) is used for containing OPs solution (3) to be detected; A first through hole (101) is formed in the right side plate of the infiltration groove (1) and penetrates left and right, a second through hole (102) is formed in the left side plate of the infiltration groove (1) and penetrates left and right, and the first through hole (101) and the second through hole (102) correspond to each other left and right; Third and fourth turning rollers (10) and (11) are rotatably connected between the front and rear side plates of the infiltration groove (1) and are arranged left and right, and the top of each of the third and fourth turning rollers (10) and (11) is lower than the first and second through holes (101) and (102); One end of the test strip (2) passes through the first through hole (101), winds around the bottom of the third and fourth turning rollers (10) and (11), and then passes out from the second through hole (102); The test strip (2) is provided with a detection area, the detection area is provided with T line areas (202), C line areas (203), PH test paper segments (204) and blank segments (201) located on the left and right outer sides of the T line areas (202) and the PH test paper segments (204) and arranged left and right, the T line areas (202), the C line areas (203) and the PH test paper segments (204) are located between the third and fourth turning rollers (10) and (11), and the left and right outer blank segments (201) are respectively arranged in the second through hole (102) and the first through hole (101); The T line area (202) comprises, from bottom to top, a backing card, a sample pad, a UCNPs-mAbs layer and a GO-Ag layer, the UCNPs-mAbs layer comprises a NaYF4:Yb,Er@NaYF4 layer as a fluorescent donor and OPs monoclonal antibodies modified on the surface of the NaYF4:Yb,Er@NaYF4 layer, and the GO-Ag layer comprises a GO layer as a fluorescent acceptor and OPs antigens coupled to the surface of the GO layer; The C line area (203) comprises, from bottom to top, a backing card, a sample pad and a UCNPs layer, and the UCNPs layer comprises a NaYF4:Yb,Er@NaYF4 layer.
2. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 1, characterised in that: The test strip (2) is provided with a plurality of detection areas connected left and right, the detection areas comprise T line areas (202), C line areas (203), PH test paper segments (204) and blank segments (201) arranged left and right; The two blank segments (201) of the detection area located at the left end are respectively arranged in the second through hole (102) and the first through hole (101).
3. A dipstick device for a sample cell for detecting organophosphorus pesticides according to claim 1 or 2, characterised in that: A plurality of T line areas (202), one C line area (203) and one PH test paper segment (204) are arranged left and right in the detection area. Different types of OPs monoclonal antibodies are modified in the UCNPs-mAbs layer in each T line area (202), and OPs antigens capable of specifically binding to the OPs monoclonal antibodies in the area are modified in the GO-Ag layer in each T line area (202).
4. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 3, characterised in that: A winding drum device (4) is fixedly arranged on the right side of the infiltration groove (1), the right end of the test strip (2) is wound on the winding drum device (4), and the winding drum device (4) is located below the first through hole (101). The right side plate of the infiltration tank (1) is provided with a first positioning device for clamping and fixing the test paper strip (2), and the first positioning device is located between the first through hole (101) and the winding device (4); The left side plate of the infiltration tank (1) is provided with a second positioning device for clamping and fixing the test paper strip (2), and the second positioning device is located below the second through hole (102).
5. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 4, characterised in that: The first positioning device comprises a first magnet block (5) embedded in the right side plate of the infiltration tank (1), the right side of the first magnet block (5) is adsorbed with a detachable second magnet block (6), the second magnet block (6) is provided with a first handle (7), and the test paper strip (2) is arranged between the first magnet block (5) and the second magnet block (6); The second positioning device comprises a third magnet block (18) embedded in the left side plate of the infiltration tank (1), the left side of the third magnet block (18) is adsorbed with a detachable fourth magnet block (19), the fourth magnet block (19) is provided with a second handle (20), and the test paper strip (2) is arranged between the third magnet block (18) and the fourth magnet block (19).
6. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 5, characterised in that: The front and rear side plates of the infiltration tank (1) are rotatably connected with the second steering roller (9) and the fifth steering roller (12) arranged at intervals left and right; The second steering roller (9) is located above the right of the third steering roller (10), and the upper end of the second steering roller (9) is flush with the bottom of the first through hole (101); The fifth steering roller (12) is located above the left of the fourth steering roller (11), and the upper end of the fifth steering roller (12) is flush with the bottom of the second through hole (102); The left end of the test paper strip (2) sequentially passes through the first through hole (101), the second steering roller (9), the third steering roller (10), the fourth steering roller (11), the fifth steering roller (12) and the second through hole (102) from right to left.
7. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 6, characterised in that: The right side plate of the infiltration tank (1) is fixedly provided with a second mounting frame, the first steering roller (8) extending in the front-rear direction is rotatably arranged on the second mounting frame, the first steering roller (8) is located between the first positioning device and the first through hole (101), and the top of the first steering roller (8) is flush with the bottom of the first through hole (101); The left end of the test paper strip (2) sequentially passes through the first steering roller (8), the first through hole (101), the second steering roller (9), the third steering roller (10), the fourth steering roller (11), the fifth steering roller (12) and the second through hole (102) from right to left.
8. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 2, wherein: The third through hole (104) is communicated with the second through hole (102), the top of the third through hole (104) is communicated with the outside, the cutting device capable of moving up and down is arranged in the third through hole (104), and the upper end of the cutting device extends to the outside of the third through hole (104); The bottom of the second through hole (102) is fixedly provided with a backing plate (103), and the cutting device and the backing plate (103) cooperate to cut the test paper strip (2).
9. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 8, characterised in that: The inner side wall of the third through hole (104) is fixedly provided with a limiting block (105), a plurality of movable holes passing through the front and rear and the upper and lower are arranged on the limiting block (105); A cutter (16) extending in the front-rear direction is arranged below the limiting block (105), and the front-rear length of the cutter (16) is greater than the front-rear width of the test strip (2); A plurality of first connecting rods (14) are fixedly arranged on the top of the cutter (16) and spaced apart in the front-rear direction, and a sliding block is fixedly arranged on the first connecting rod (14) and in sliding contact with the left and right inner walls of the third through hole (104); a reset spring (15) is sleeved on the first connecting rod (14), the lower end of the reset spring (15) abuts against the limiting block (105), the upper end of the reset spring (15) abuts against the bottom of the sliding block, the upper end of the first connecting rod (14) penetrates out of the third through hole (104), and the upper end of the first connecting rod (14) is fixedly connected with a pressing plate (13); When the reset spring (15) is in the initial state, the lower end of the cutter (16) is located in the third through hole (104).
10. A dipstick device for a sample cell for detecting organophosphorus pesticides as claimed in claim 1, wherein: A timer (21) is fixedly arranged on the infiltration tank (1).