Strain breeding culture device

Abstract

The utility model discloses a strain breeding culture device which comprises a cabinet body, a plurality of layers of limiting plates are arranged in the cabinet body in parallel, fixing grooves used for containing culture media are formed in the limiting plates, and swing units used for driving the limiting plates to synchronously swing up and down are arranged in the cabinet body. According to the device, centrifugal force interference is eliminated through the up-down synchronous swing technology, uniform suspension of strains and stable mixing of culture medium components are achieved, meanwhile, the oxygen dissolving efficiency and operation convenience are improved, and the strict requirement of the biopharmaceutical industry for the high-homogeneity culture environment is met.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of microorganism culture equipment, specifically relates to a strain breeding culture device. BACKGROUND

[0002] In the process of microorganism research and strain breeding, air circulation is crucial for the growth and reproduction of strains. In order to improve the air circulation effect, the existing technology usually adopts the design of mechanical rotation combined with forced ventilation to improve the oxygen dissolution efficiency. For example, the utility model patent CN209178377U discloses a high-efficiency strain breeding culture device, which drives the test bottle to rotate at low speed through the driving mechanism, and cooperates with the air blower to spray sterile air to the placing piece, so as to increase the contact area of the strain and the air. Similarly, the utility model patent CN210620794U drives the rotating shaft to rotate the sample placing rack through the motor, and adjusts the sample position dynamically and combines the sterile air tank to improve the oxygen dissolution rate.

[0003] However, the above technical solutions all rely on rotating motion to realize the shaking of the strain, which can improve the oxygen dissolution effect, but the centrifugal force generated in the rotating process will cause the bacteria to gather to the outside of the container, resulting in uneven distribution of the strain. Especially for the culture medium with high viscosity, the centrifugal action easily causes the stratified settlement of the nutrient components, resulting in local nutrient deficiency of the strain, affecting the growth uniformity. The uneven distribution of the strain leads to the fluctuation of the concentration of metabolic products, directly reducing the synthesis efficiency of the target product (such as antibiotics, enzyme preparations or vaccine components); and the stratified settlement of the nutrient components will exacerbate the heterogeneity of the culture system, causing batch-to-batch differences in scale-up production, increasing the difficulty of quality control, and even prolonging the fermentation period due to the instability of the strain activity, significantly increasing the cost of biopharmaceutical research and development.

[0004] Therefore, it is urgent to design a strain breeding culture device to meet the experimental needs of the current biopharmaceutical industry. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a strain breeding culture device, which can eliminate the interference of centrifugal force through up-down synchronous swinging technology, realize uniform suspension of the strain and stable mixing of the culture medium components, improve the oxygen dissolution efficiency and operation convenience, and meet the stringent needs of the biopharmaceutical industry for high-homogeneity culture environment.

[0006] The utility model solves the above problems by adopting the following technical scheme: a strain breeding culture device, comprising a cabinet body, a plurality of limiting plates are arranged in parallel in the cabinet body, a plurality of fixing grooves for placing culture medium are formed in the limiting plates, and a swinging unit is arranged in the cabinet body for driving all the limiting plates to swing up and down synchronously.

[0007] Preferably: the swing unit comprises a base, two groups of supports are arranged on the left and right sides of the base, a plurality of groups of connecting frames are vertically and equidistantly arranged between the two groups of supports, the connecting frames are provided with sliding grooves matched with the sliding connection of the limiting plates, the connecting frames are respectively hinged through the first connecting rods and the second connecting rods arranged in parallel with the supports, the lower end of the second connecting rod is provided with a limiting groove, the limiting groove is slidably hinged with a crankshaft, and the crankshaft is matched with a driving motor for driving the rotation of the crankshaft.

[0008] Preferably: the cabinet top is provided with a circular groove, a plurality of groups of air holes are equidistantly arranged in the circular groove in a circumferential direction, and a cover plate for shielding the air holes is rotatably arranged in the circular groove.

[0009] Preferably: the rear end of the limiting plate is provided with a magnet, and the rear side of the connecting frame is provided with an iron plate attracted to the magnet.

[0010] Preferably: a storage drawer is arranged in the cabinet.

[0011] Preferably: an adjusting handle is arranged above the cover plate.

[0012] Preferably: a plurality of square partitions are arranged in the storage drawer, and the partitions are isolated from each other by interval plates.

[0013] Preferably: the storage drawer is provided with a handle.

[0014] Preferably: four groups of supporting legs and a group of moving wheels rotatably arranged at the lower end of the supporting legs are arranged below the cabinet.

[0015] Preferably: a cabinet door is hinged to the front side of the cabinet, and the cabinet door is provided with a transparent window.

[0016] Compared with the prior art, the utility model has the following advantages and effects:

[0017] The utility model discloses a kind of bacterial strain breeding and cultivation devices, which comprises cabinet body, swing unit, limiting plate, storage drawer and cabinet door, wherein cabinet body is provided with base, and swing unit is arranged on the base. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 This is a schematic diagram of the internal structure of the cabinet in an embodiment of this utility model.

[0019] Figure 2 This is a schematic diagram of the structure of the swing unit in an embodiment of this utility model.

[0020] Figure 3 yes Figure 2 A partial structural diagram of the swing unit.

[0021] Figure 4 This is a schematic diagram of the installation structure of the limiting plate and the connecting frame in an embodiment of this utility model.

[0022] Figure 5 This is a schematic diagram of the connection structure between the cabinet and the cover plate in an embodiment of this utility model.

[0023] Figure 6 This is a three-dimensional structural diagram of the cabinet in an embodiment of this utility model.

[0024] Attached Figures: Cabinet 11, Limiting Plate 12, Fixing Slot 13, Swing Unit 14, Base 21, Bracket 22, Connecting Frame 23, Slide 24, First Link 25, Second Link 26, Limiting Slot 27, Crankshaft 28, Drive Motor 29, Magnet 31, Iron Plate 32, Circular Slot 33, Ventilation Hole 34, Cover Plate 35, Through Hole 36, Adjustment Handle 37, Pipeline 38, Fan 39, Storage Drawer 41, Partition 42, Divider Plate 43, Handle 44, Support Leg 45, Caster Set 46, Cabinet Door 47, Transparent Window 48. Detailed Implementation

[0025] The present invention will be further described in detail below with reference to the accompanying drawings and through embodiments. The following embodiments are explanations of the present invention, but the present invention is not limited to the following embodiments.

[0026] Example:

[0027] See Figure 1 and Figure 2 In this embodiment, a microbial strain selection and cultivation device is involved, which is specifically used for the dynamic cultivation of microbial strains. It replaces the traditional rotation and shaking method with up-and-down synchronous swinging technology to solve the problems of bacterial aggregation and culture medium stratification caused by centrifugal force. Its specific structure includes a cabinet 11, on which several layers of limiting plates 12 are arranged in parallel. Several fixing slots 13 for placing culture medium are opened in the limiting plates 12. The cabinet 11 is provided with a swinging unit 14 for driving all the limiting plates 12 to swing up and down synchronously.

[0028] Specifically, in this embodiment, the cabinet 11 has a cuboid structure with four parallel layers of limiting plates 12 (made of corrosion-resistant aluminum alloy) arranged inside. Each limiting plate 12 has a cylindrical fixing groove 13 for placing standard bacterial culture dishes or test tubes (culture medium containers). The limiting plates 12 are driven to swing synchronously up and down by the swing unit 14. Since the up-and-down swing mode only generates vertical inertia (force), it avoids the horizontal centrifugal force of traditional rotating devices, making the bacteria uniformly suspended in the culture medium. The vertical fluctuation generated by the up-and-down swing allows the components in the culture medium to come into better contact and mix with the air in the cabinet 11 after shaking, improving the dissolved oxygen rate (the vertical inertial force forces the liquid to generate periodic wave motion, enhancing the turbulence at the gas-liquid interface, making oxygen dissolve in the culture medium more efficiently). Compared with the method of rotational centrifugation, the up-and-down swing of this invention makes it less likely for viscous liquids (such as agar medium) to settle and stratify, which is beneficial to maintaining the uniformity and stability of nutrient composition. This embodiment, through the synchronous swing structure of the limiting plate 12, fundamentally avoids the defects of bacterial aggregation and culture medium stratification caused by centrifugal force in the prior art through the rotating device, thus meeting the requirements for microbial cultivation in biopharmaceuticals.

[0029] In this embodiment, the swing unit 14 includes a base 21. Two sets of supports 22 are arranged on the left and right sides of the base 21. Several sets of connecting frames 23 are rotatably arranged at equal vertical intervals between the two sets of supports 22. Each connecting frame 23 has a sliding groove 24 adapted to slide with the limiting plate 12. The connecting frames 23 are hinged together by a first connecting rod 25 and a second connecting rod 26 arranged parallel to the supports 22, forming a parallelogram mechanism. See also... Figure 3 The lower end of the second connecting rod 26 is provided with a limiting groove 27, which is slidably hinged to a crankshaft 28. The crankshaft 28 is fitted with a drive motor 29 that drives its rotation. In this embodiment, the crankshaft 28 is driven to rotate by the drive motor 29, and the second limiting rod moves up and down through the limiting groove 27, causing the connecting frame 23 to swing up and down around its rotational connection point with the bracket 22.

[0030] See Figure 4 In this embodiment, a magnet 31 is provided at the rear end of the limiting plate 12, and an iron plate 32 that attracts the magnet 31 is provided at the rear side of the connecting frame 23. During installation, the limiting plate 12 is slid into the connecting frame 23 through the slide groove 24, and then the magnet 31 and the iron plate 32 attract each other to achieve the installation and fixation between the limiting plate 12 and the connecting frame 23, so as to prevent the limiting plate 12 from accidentally falling off when the connecting frame 23 swings.

[0031] See Figure 5The cabinet 11 has a circular groove 33 on its top, and several sets of ventilation holes 34 are evenly spaced around the circumference of the groove 33. The groove 33 is rotatably fitted with a cover plate 35 for blocking the ventilation holes 34. The cover plate 35 has through holes 36 that are adapted to the size and position of the ventilation holes 34. An adjustment handle 37 is provided above the cover plate 35. By rotating the adjustment handle 37, the overlap between the through holes 36 of the cover plate 35 and the ventilation holes 34 of the cabinet 11 is adjusted to achieve oxygen supply control (such as closing the ventilation holes 34 when anaerobic bacteria are being cultured). This is combined with the cabinet ventilation system (in this embodiment, the cabinet 11 is connected to a fan 39 via a pipe 38, which can draw air from the outside. A HEPA filter and an ultraviolet sterilization lamp can be installed at the air inlet of the fan 39. The air enters the cabinet after being purified by the HEPA filter and the ultraviolet sterilization lamp, and finally exits from the ventilation holes 34 on the top of the cabinet 11).

[0032] The cabinet 11 contains a storage drawer 41, which slides beneath the base 21. The drawer 41 has several square compartments 42, separated by partitions 43. When closed, the drawer 41 forms a relatively sealed space against the base 21, suitable for cultivating microorganisms under anaerobic or light-protected conditions. The drawer 41 is equipped with a handle 44 for easy pulling.

[0033] See Figure 6 The cabinet 11 is equipped with four sets of support legs 45 and a set of casters 46 rotatably mounted on the lower end of the support legs 45. The casters 46 allow operators to easily move the cabinet 11 and adjust its position, providing good portability.

[0034] The cabinet 11 is hinged to a cabinet door 47 on the front side, and the cabinet door 47 is provided with a transparent viewing window 48. The transparent viewing window 48 allows users to make preliminary observations of the growth status of the microorganisms and environmental parameters (such as the turbidity of the culture medium and the formation of the microbial film) from the outside of the cabinet 11 without having to frequently open and close the cabinet door 47.

[0035] The above description in this specification is merely illustrative of the present invention. Those skilled in the art to which this invention pertains may make various modifications or additions to the described specific embodiments or use similar methods to replace them, as long as they do not depart from the content of this specification or exceed the scope defined in the claims, all of which shall fall within the protection scope of this invention.

Claims

1. A strain selection and cultivation device, comprising a cabinet, characterized in that, The cabinet is provided with several layers of limiting plates arranged in parallel. Each limiting plate has a fixed groove for placing culture medium. The cabinet is also provided with a swing unit for driving all the limiting plates to swing up and down synchronously.

2. The strain selection and cultivation device according to claim 1, characterized in that: The swing unit includes a base, with two sets of supports on the left and right sides of the base. Several sets of connecting frames are rotatably arranged vertically at equal intervals between the two sets of supports. The connecting frames have sliding grooves adapted to slide with the limiting plate. The connecting frames are hinged to each other by a first connecting rod and a second connecting rod arranged parallel to the supports. The lower end of the second connecting rod is provided with a limiting groove, which is slidably hinged to a crankshaft. The crankshaft is adapted to a drive motor that drives its rotation.

3. The strain selection and cultivation device according to claim 1, characterized in that: The cabinet has a circular groove on its top, and several sets of ventilation holes are equally spaced around the circumference of the groove. The groove is rotatably fitted with a cover plate to block the ventilation holes, and the cover plate has through holes of the size and position of the ventilation holes.

4. The strain selection and cultivation device according to claim 2, characterized in that: The limiting plate is equipped with a magnet at its rear end, and the connecting frame is equipped with an iron plate that attracts the magnet at its rear side.

5. The strain selection and cultivation device according to claim 2, characterized in that: The cabinet is equipped with storage drawers, which are slidably positioned below the base.

6. The strain selection and cultivation device according to claim 3, characterized in that: An adjustment handle is provided on the top of the cover plate.

7. The strain selection and cultivation device according to claim 5, characterized in that: The storage drawer is equipped with several square compartments, which are separated from each other by partitions.

8. The strain selection and cultivation device according to claim 5, characterized in that: The storage drawer is equipped with a handle.

9. The strain selection and cultivation device according to claim 1, characterized in that: The cabinet is equipped with four sets of support legs and a set of casters that are rotatably mounted on the lower end of the support legs.

10. The strain selection and cultivation device according to claim 1, characterized in that: The cabinet body is hinged to the front side with a cabinet door, and the cabinet door is equipped with a transparent viewing window.

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