Kit for detecting lipase in dairy product based on paper-based micro-fluidic chip

Abstract

The utility model discloses a kit for detecting lipase in dairy products based on a paper-based micro-fluidic chip, and belongs to the technical field of biological detection. The kit comprises a kit body, a flip cover and a detachably mounted lipase detection device. The detection device is composed of a hydrophobic acrylic fixing plate and a paper-based micro-fluidic chip, the fixing plate is provided with a non-penetrating inclined shallow groove, and the groove comprises a triangular liquid dropping area, a strip lane and a circular detection color development area which are respectively used for sample loading, liquid guiding and color development reaction. The chip structure is optimized, and the capillary action and gravity are utilized to synergistically promote liquid flow, so that the detection sensitivity and accuracy are improved; the triangular and circular partition design can avoid operation mistaken dripping. The box body is provided with a positioning groove and a positioning bump to ensure the stability of the detection device. The kit has the advantages of portability, simplicity and convenience in operation, low cost and the like, is suitable for rapid field detection of lipase in dairy products, and has a good application prospect.

Description

Technical Field

[0001] This invention relates to lipase detection, specifically to a kit for detecting lipase in dairy products based on a paper-based microfluidic chip. Background Technology

[0002] Lipases are enzymes produced by spoilage bacteria in dairy products, and their presence poses a serious threat to the quality and safety of these products. Lipases break down fat molecules in dairy products, leading to a series of adverse changes. First, lipase activity causes fat to rise to the surface, resulting in an uneven texture and affecting the taste and appearance of the dairy product. Second, lipase activity also leads to acidification of the dairy product, causing a decrease in pH, further affecting its flavor and stability. Furthermore, lipase activity produces a rancid odor, which is not only unpleasant but also reduces the market acceptance of the dairy product. Traditional lipase detection methods typically rely on complex chemical analysis and equipment, which are often cumbersome, time-consuming, and require expensive equipment and specialized laboratory environments, thus limiting their application in rapid on-site testing.

[0003] In recent years, paper-based microfluidics technology has gradually become an important development direction in the field of biosensors due to its low cost, convenience, and high efficiency. Paper-based microfluidic chips utilize the capillary phenomenon of paper materials to achieve automatic liquid flow without the need for external pumping devices, which makes paper-based microfluidic chips have great application potential in portable diagnostic devices.

[0004] Therefore, there is an urgent need for a new, simple, and efficient method for lipase detection that can improve detection sensitivity, simplify the operation process, and reduce costs. Utility Model Content

[0005] This invention provides a reagent kit for detecting lipase based on a paper-based microfluidic chip. Through simplified structure and optimized design, it enables rapid and convenient lipase detection. The specific solution is as follows:

[0006] A kit for detecting lipase in dairy products based on a paper-based microfluidic chip, the kit comprising a box body and a flip-top, one side of the flip-top being rotatably mounted on the top side of the box body, and the other side of the flip-top being snap-fitted to the other side of the top of the box body. A lipase detection device is detachably installed within the kit, the lipase detection device comprising:

[0007] A fixing plate is detachably installed inside the box. The inner side of the box is provided with a groove for supporting the fixing plate. The fixing plate is provided with multiple non-through shallow grooves, each shallow groove including an elongated channel and a triangular area and a circular area respectively connected to both ends of the elongated channel.

[0008] A paper-based microfluidic chip, the paper-based microfluidic chip having the same shape and size as the shallow groove and placed at the bottom of the shallow groove, the paper-based microfluidic chip having a triangular droplet area, a long lane and a circular detection and color development area respectively located in the triangular area, the long channel and the circular area.

[0009] Furthermore, the inner wall of the box is provided with a positioning groove, and the fixing plate is fixed with a positioning protrusion that is slidably disposed in the positioning groove.

[0010] Furthermore, the tray is located at the bottom inside the box, and vertical positioning grooves extending downward to the tray are provided on both sides of the inner wall of the box.

[0011] Multiple lipase detection devices are installed in the kit in a stacked manner. Each set of lipase detection devices has positioning protrusions that slide in the vertical positioning groove on both sides of the fixing plate.

[0012] Furthermore, the fixing plate is made of hydrophobic acrylic sheet.

[0013] Furthermore, the depth of the shallow groove is 0.5-2 mm.

[0014] Furthermore, the side length of the triangular droplet area is 3-5 mm, the width of the lane is 0.5-1 mm, and the diameter of the circular detection and color development area is 3-5 mm.

[0015] Furthermore, the fixing plate is provided with a through hole for a handle.

[0016] Furthermore, the box body is equipped with a "U"-shaped handle, and the two sides of the handle are rotatably connected to the two sides of the box body.

[0017] Furthermore, each of the shallow grooves is inclined within the fixed plate, and the triangular area to the circular area of ​​each shallow groove gradually slopes downward.

[0018] Furthermore, the fixing plate is provided with a transparent sheet covering the circular area.

[0019] The advantages of this utility model are:

[0020] 1. This invention combines lipase detection with paper-based microfluidic technology, utilizing the capillary action of paper material to achieve automatic liquid flow and thus realize lipase detection, greatly simplifying the operation steps and improving detection efficiency.

[0021] 2. The fixing plate has multiple non-through shallow grooves for placing paper-based microfluidic chips, which not only provides stable fixation but also facilitates observation and reading of test results.

[0022] 3. By optimizing the structural design of the paper-based microfluidic chip, including the triangular dropper area, the long lane, and the circular detection and color development area, the detection of lipase is made more sensitive and accurate. Using different shapes for the dropper area and the color development area can effectively avoid accidental drop of sample solution into the color development area.

[0023] 4. The kit comes with a flip-top box for easy carrying and storage. The box also has slots and positioning grooves to support and fix the lipase detection device, ensuring the stability and accuracy of the detection process.

[0024] 5. The kit can be equipped with multiple lipase detection devices to achieve batch detection, further improving detection efficiency. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is an image of the reagent kit for detecting lipase in dairy products based on a paper-based microfluidic chip, according to this invention.

[0027] Figure 2 This is a schematic diagram of the reagent kit after opening the flip-top.

[0028] Figure 3 This is a schematic diagram of the fixation plate inside the reagent kit and the paper-based microfluidic chip mounted on the fixation plate. Detailed Implementation

[0029] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.

[0030] To fully understand this utility model, detailed steps and structures will be presented in the following description to illustrate the technical solution of this utility model. Preferred embodiments of this utility model are described in detail below; however, in addition to these detailed descriptions, this utility model may have other embodiments.

[0031] Reference Figure 1-3As shown, this invention provides a kit for detecting lipase in dairy products based on a paper-based microfluidic chip 11. The kit includes a box body 1, which consists of a box body 2 and a flip cover 3. One side of the flip cover 3 is rotatably mounted on the top side of the box body 1, and the other side of the flip cover 3 is connected to the top of the box body via a snap-fit. A lipase detection device is detachably installed inside the kit, and the lipase detection device includes a fixing plate 6 and a paper-based microfluidic chip 11.

[0032] The fixing plate 6 is detachably installed inside the housing 1. The inner side of the housing 1 is provided with a groove for supporting the fixing plate 6. The fixing plate 6 has multiple non-through shallow grooves 7. The shallow grooves 7 include elongated channels and triangular and circular areas connected to the two ends of the elongated channels, respectively. The paper-based microfluidic chip 11 has the same shape and size as the shallow grooves 7 and is placed at the bottom of the shallow grooves 7. The paper-based microfluidic chip 11 has triangular droplet areas 9, elongated lanes 10, and circular detection color development areas 8 located in the triangular areas, elongated channels, and circular areas, respectively. The circular detection color development area 8 is provided with a chemical reagent that can produce a color reaction with lipase, such as: 1. Nitrophenol esters (nitrophenol butyrate); 2. Thiocholine esters (such as thiobutyrylcholine); 3. Copper soap method (copper acetate).

[0033] In an optional embodiment, the inner wall of the box body 1 is provided with a positioning groove 4, and the fixing plate 6 is fixed with a positioning protrusion 5 that is slidably disposed in the positioning groove 4. The positioning protrusion 5 cooperates with the positioning groove 4, so that the fixing plate 6 can be more stable when installed in the box body 1, and is less prone to shaking or misalignment. It should be noted that the support groove is used to support the fixing plate 6, and the positioning groove 4 is used for the longitudinal positioning of the fixing plate 6.

[0034] In an optional embodiment, the fixing plate 6 is made of hydrophobic acrylic sheet, which can effectively prevent the sample liquid from spreading in non-specific areas on the surface of the fixing plate, ensuring the accuracy of the test results. The depth of the shallow groove 7 is 0.5-2mm, preferably 1mm.

[0035] In an optional embodiment, the triangular droplet area 9 has a side length of 3-5 mm, the lane 10 has a width of 0.5-1 mm, and the circular detection and color development area 8 has a diameter of 3-5 mm. Using a triangle for the droplet area and a circle for the detection area effectively prevents operator error and avoids accidentally dripping sample solution into the detection area.

[0036] In an optional embodiment, each shallow groove 7 is inclined within the fixed plate 6, with the triangular area of ​​each shallow groove 7 gradually sloping downwards towards the circular area. This inclined design allows the sample solution dropped into the triangular droplet area 9 to flow more quickly into the circular detection and color development area 8 under the influence of gravity, improving the accumulation efficiency of the sample solution within the circular area and facilitating subsequent detection operations. Furthermore, the inclined design of the shallow groove 7 also guides the sample solution along the path of the lane 10, ensuring that the sample solution is ultimately evenly distributed within the circular area, further enhancing the accuracy and reliability of the detection.

[0037] Furthermore, a transparent sheet is provided on the fixing plate 6 to cover each of the circular areas, so as not to affect the observation of the test results and to prevent the circular detection color development area 8 from being contaminated.

[0038] In an optional embodiment, the fixing plate 6 is provided with a handle through hole 12, which facilitates the removal of the fixing plate 6 from the reagent kit and also facilitates the accurate placement of the fixing plate 6 into the reagent kit.

[0039] In an optional embodiment, the tray is located at the bottom inside the box body 1, and vertical positioning grooves 4 extending downward to the tray are provided on both sides of the inner wall of the box body 1; multiple sets of lipase detection devices are installed in the kit in a stacked manner, and each set of lipase detection devices has positioning protrusions 5 slidably disposed in the vertical positioning grooves 4 on both sides of the fixing plate 6.

[0040] In an optional embodiment, the box body 1 is equipped with a U-shaped handle (not shown in the figure), with both sides of the handle rotatably connected to the sides of the box body 1. The U-shaped handle design allows users to easily carry or move the reagent kit, increasing ease of use. Simultaneously, the rotatable connection of the U-shaped handle allows it to be fitted snugly against the side of the box body 1 when not in use, without occupying extra space and maintaining the overall aesthetics and compactness of the reagent kit.

[0041] The operating principle of this utility model is described as follows: When in use, first open the flip cover 3, fix the fixing plate 6 with the positioning groove 4 on the inner side of the box body 2 through the positioning protrusion 5, then put the paper-based microfluidic chip 11 into the shallow groove 7 on the fixing plate 6, and then add the sample to be detected to the triangular drop area 9. After the sample diffuses through the lane 10 to the circular detection color development area 8, and reacts with the color developer on the circular detection color development area 8 to produce a corresponding color change, the detection is completed. Note that the reagent kit should be kept flat throughout the process.

[0042] The preferred embodiments of this utility model have been described above. It should be understood that this utility model is not limited to the specific embodiments described above. Devices and structures not described in detail herein should be understood as being implemented in a conventional manner within the art. Any person skilled in the art can make many possible variations and modifications to the technical solutions of this utility model using the disclosed methods and techniques, or modify them into equivalent embodiments with equivalent changes, without departing from the scope of the technical solution of this utility model. This does not affect the essential content of this utility model. Therefore, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model, without departing from the content of the technical solution of this utility model, still fall within the protection scope of the technical solution of this utility model.

Claims

1. A kit for detecting lipase in dairy products based on a paper-based microfluidic chip, the kit comprising a box body and a flip cover, one side of the flip cover being rotatably mounted on the top side of the box body, and the other side of the flip cover being snap-fitted to the other side of the top of the box body, characterized in that... A lipase detection device is detachably installed within the kit, the lipase detection device comprising: A fixing plate is detachably installed inside the box. The inner side of the box is provided with a groove for supporting the fixing plate. The fixing plate is provided with multiple non-through shallow grooves, each shallow groove including an elongated channel and a triangular area and a circular area respectively connected to both ends of the elongated channel. A paper-based microfluidic chip, wherein the shape and size of the paper-based microfluidic chip are the same as those of the shallow groove and are placed at the bottom of the shallow groove, the paper-based microfluidic chip is provided with a triangular droplet area, a long strip channel and a circular area respectively located in the triangular area, the long strip channel and the circular area, and a circular detection color development area, wherein the circular detection color development area is provided with a chemical reagent that produces a color reaction with lipase.

2. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1, characterized in that, The inner wall of the box is provided with a positioning groove, and the fixing plate is fixed with a positioning protrusion that is slidably disposed in the positioning groove.

3. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 2, characterized in that, The bracket is located at the bottom inside the box, and vertical positioning grooves extending downward to the bracket are provided on both sides of the inner wall of the box. Multiple lipase detection devices are installed in the kit in a stacked manner. Each set of lipase detection devices has positioning protrusions that slide in the vertical positioning groove on both sides of the fixing plate.

4. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1, characterized in that, The fixing plate is made of hydrophobic acrylic sheet.

5. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1, characterized in that, The depth of the shallow groove is 0.5-2mm.

6. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1 or 5, characterized in that, The side length of the triangular droplet area is 3-5 mm, the width of the lane is 0.5-1 mm, and the diameter of the circular detection and color development area is 3-5 mm.

7. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1 or 3, characterized in that, The fixing plate has a through hole for a handle.

8. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1, characterized in that, The box is equipped with a "U"-shaped handle, and the two sides of the handle are rotatably connected to the two sides of the box.

9. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1, characterized in that, Each of the shallow grooves is inclined within the fixed plate, and the triangular area of ​​each shallow groove gradually slopes downwards towards the circular area.

10. The kit for detecting lipase in dairy products based on a paper-based microfluidic chip as described in claim 1 or 9, characterized in that, The fixing plate is provided with a transparent sheet covering the circular area.

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