Suction type tobacco leaf ultrastructure sampler

Abstract

The utility model discloses a suction type tobacco leaf ultrastructure sampler, which comprises a sampling barrel, a suction tube, a suction tube, a suction tube, a suction tube, a suction tube, a suction tube and a suction tube, the air suction cylinder is provided with a lower port and an upper port, and the lower port is connected with the connecting port; the piston head is mounted in the air suction cylinder; the pressing rod is installed on the upper end face of the piston head, and the upper end of the pressing rod extends upwards out of the upper end opening; the first spring is sleeved outside the pressing rod; in a natural state, under the action of the first spring, the pressing rod drives the piston head to move upwards in the air suction barrel; before sampling, the piston head is driven by the pressing rod to move downwards for a certain distance, then a part of glutaraldehyde solution is sucked through the sampling opening of the sampling barrel, the sampling opening is aligned to the position to be sampled on tobacco leaves and is pressed down for cutting, the sample is located at the sampling opening, and then the sampler is moved to a storage bottle. And the glutaraldehyde solution and the sample in the sampling barrel can be simultaneously pushed to the storage bottle to be stored by pushing the pressing rod downwards, so that timely preservation is realized.

Description

Technical Field

[0001] This utility model relates to an absorption-type tobacco leaf ultra-microstructure sampler, belonging to the field of tobacco leaf sample sampling and testing technology. Background Technology

[0002] In tobacco leaf studies, electron microscopy reveals nanoscale particles and specific arrangement patterns in tissues such as cell wall epidermis and oothecae. These ultrastructures are closely related to plant stress resistance mechanisms.

[0003] As a model organism, tobacco's leaf ultrastructure holds unique value in several aspects. For example, under abiotic stresses (such as drought and high temperature), water loss in leaf cells, changes in membrane systems, and adjustments in metabolic activity can be directly observed through ultrastructure, providing important evidence for studying plant stress resistance. Furthermore, the abundant organic components in tobacco leaves, such as resinous and glycosuria networks, exhibit a unique three-dimensional distribution under ultrastructure, further revealing the function and metabolic activity of plant cell walls.

[0004] By observing the ultrastructure of leaves under different environments, conditions, and growth stages, we can reveal the dynamic changes in plant cell division, differentiation, and organelles, as well as elucidate the mechanisms of plant abiotic stress and its biological processes. For example, observing the initial structure of leaf cells and the development of chloroplasts during the tobacco seedling stage helps to understand the early establishment mechanism of photosynthesis. Analyzing the changes in leaf cell organs, cell expansion, and cell wall thickening during the leaf leaf growth stage and vigorous growth stage can provide scientific and technological support for tobacco biomass accumulation and genetic breeding.

[0005] The formation of tobacco quality is determined by the coordinated action of ultrastructure, accumulation of chemical components, and synthesis of aroma substances in tobacco leaves. The number, size, and distribution of organelles such as chloroplasts (starch granules) and proteosomes vary among tobacco leaves of different qualities. The formation of high-quality tobacco leaves may require a more rational leaf cell structure that facilitates the synthesis and storage of substances such as sugars and nicotine. Ultrastructure sampling and observation can explore the root causes of these differences, providing a basis for improving tobacco quality.

[0006] Traditional methods for sampling the ultrastructure of tobacco leaves typically involve cutting the leaves with a hand blade, placing the sample in a storage bottle, and then mixing it with glutaraldehyde solution for preservation. However, this sampling method has the following problems: firstly, the size of the extracted samples is inconsistent, which significantly affects the experiment; secondly, it is impossible to preserve the samples in a timely manner. Utility Model Content

[0007] Based on the above, this utility model provides an absorption-type tobacco leaf microstructure sampler that can quickly fix fresh samples by pre-storing glutaraldehyde, thereby overcoming the shortcomings of the prior art.

[0008] The technical solution of this utility model is: an absorption-type tobacco leaf ultra-microstructure sampler, comprising:

[0009] A sampling tube has a sampling port and a connection port, wherein the sampling port has a blade;

[0010] An air extraction cylinder has a lower port and an upper port, the lower port being connected to the connection port;

[0011] The piston head is installed inside the suction cylinder;

[0012] A pressing rod is installed on the upper end face of the piston head, and the upper end of the pressing rod extends upward out of the upper port.

[0013] The first spring is fitted onto the outside of the pressing rod;

[0014] In its natural state, under the action of the first spring, the pressing rod drives the piston head to move upward inside the air extraction cylinder.

[0015] In one example, the vacuum pump includes a spring chamber and a piston chamber installed at the bottom of the spring chamber. The diameter of the piston chamber is smaller than the diameter of the spring chamber. The lower port is the lower port of the piston chamber, and the upper port is the upper port of the spring chamber. The piston head is disposed in the piston chamber. The pressing rod is provided with a limiting rod, and the first spring is installed between the limiting rod and the bottom end of the spring chamber.

[0016] In one example, a cover is installed at the upper port of the spring cavity, the cover has a through hole, and the limiting rod is disposed on the rod of the pressing rod below the cover; a sliding sleeve is sleeved on the pressing rod at a position on the cover, and a second spring is provided between the sliding sleeve and the cover, the stiffness of the second spring being greater than that of the first spring.

[0017] In one example, the pressing rod includes a pressing handle, an upper section rod detachably mounted on the pressing handle, and a lower section rod detachably mounted on the upper section rod, with the limiting rod mounted on the upper section rod.

[0018] In one example, the sampling tube has a structure that is larger at the top and smaller at the bottom, and the sampling port is square.

[0019] The beneficial effects of this utility model are as follows: The sampler is composed of a sampling cylinder, a vacuum cylinder, a piston head, a pressing rod, and a first spring. Before sampling, the piston head is first driven downward by the pressing rod to move a certain distance. Then, the sampling port of the sampling cylinder is inserted into the glutaraldehyde solution. Under natural conditions, the first spring drives the pressing rod to move upward to generate negative pressure, which draws a portion of the glutaraldehyde solution into the sampling cylinder. Then, the sampling port is aligned with the position to be sampled on the tobacco leaf and pressed down to cut. At this time, the sample is located at the sampling port. Then, the sampler is moved to the storage bottle, and the pressing rod is pushed down to push the glutaraldehyde solution and the sample in the sampling cylinder into the storage bottle for storage, thus achieving timely preservation. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of a suction-type ultra-microstructure sampler.

[0021] Figure 2 This is a schematic diagram of the sampling tube.

[0022] Figure 3 This is a schematic diagram of the vacuum pump structure;

[0023] Figure 4 This is a schematic diagram of the pressing rod.

[0024] Explanation of reference numerals in the attached figures:

[0025] 1. Sampling tube; 11. Sampling port; 12. Connecting port;

[0026] 2. Air pump, 21. Lower port, 22. Upper port, 23. Spring chamber, 24. Piston chamber, 25. Cover;

[0027] 3 piston heads;

[0028] 4. Pressing rod, 41. Limiting rod, 42. Pressing handle, 43. Upper section rod, 44. Lower section rod;

[0029] 5. First spring;

[0030] 6. The second spring;

[0031] 7. Sliding sleeve. Detailed Implementation

[0032] To make the above-mentioned objectives, features, and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a full understanding of this utility model. However, this utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed below.

[0033] Please see Figures 1 to 4 This embodiment provides an absorption-type tobacco leaf microstructure sampler, comprising a sampling cylinder 1, an air extraction cylinder 2, a piston head 3, a pressing rod 4, and a first spring 5.

[0034] The sampling cylinder 1 has a structure that is larger at the top and smaller at the bottom. It has a sampling port 11 and a connecting port 12. The sampling port 11 has a blade and is square. By pressing the sampling cylinder 1 downwards, a square sample structure can be cut from the blade. The suction cylinder 2 has a lower port 21 and an upper port 22. The lower port 21 is connected to the connecting port 12. The piston head 3 is installed inside the suction cylinder 2 and can move inside the suction cylinder 2. The pressing rod 4 is installed on the upper end face of the piston head 3, and the upper end of the pressing rod 4 extends upwards to the upper port 22. The piston head 3 can be operated by pressing the rod 4. The first spring 5 is fitted outside the pressing rod 4. In the natural state, under the action of the first spring 5, the pressing rod 4 drives the piston head 3 to move upwards inside the suction cylinder 2 to the initial position.

[0035] The sampler is composed of a sampling cylinder 1, an air extraction cylinder 2, a piston head 3, a pressing rod 4, and a first spring 5. Before sampling, the piston head 3 is driven downward by the pressing rod 4. Then, the sampling port 11 of the sampling cylinder 1 is inserted into the glutaraldehyde solution. Under natural conditions, the first spring 5 drives the pressing rod 4 to move upward to generate negative pressure, drawing some glutaraldehyde solution into the sampling cylinder 1 (or even into the air extraction cylinder 2). Then, the sampling port 11 is aligned with the position to be sampled on the tobacco leaf and pressed down to cut. At this time, the sample is located at the sampling port 11. The sampler is then moved to the storage bottle, and the pressing rod 4 is pushed downward to push the glutaraldehyde solution in the sampling cylinder 1 and the sample into the storage bottle for storage, achieving timely preservation.

[0036] For ease of operation, the vacuum pump 2 includes a spring chamber 23 and a piston chamber 24 installed at the bottom of the spring chamber 23. The diameter of the piston chamber 24 is smaller than the diameter of the spring chamber 23. The lower port 21 is the lower port 21 of the piston chamber 24, and the upper port 22 is the upper port 22 of the spring chamber 23. The piston head 3 is installed inside the piston chamber 24. A limit rod 41 is fixed on the pressing rod 4. The first spring 5 is installed between the limit rod 41 and the bottom end of the spring chamber 23. Through the action of the first spring 5 and the limit rod 41, the pressing rod 4 can be compressed once.

[0037] For ease of operation, a cover 25 is installed at the upper port 22 of the spring cavity 23. A through hole is provided on the cover 25. A limiting rod 41 is installed on the rod of the pressing rod 4 below the cover 25. The pressing rod 4 passes through the through hole. A sliding sleeve 7 is sleeved on the pressing rod 4 at the position on the cover 25. A second spring 6 is provided between the sliding sleeve 7 and the cover 25. The stiffness of the second spring 6 is greater than that of the first spring 5. When the pressing rod 4 is pushed down, the first spring 5 is compressed first, while the second spring 6 is not compressed. At this time, it is used to press and absorb glutaraldehyde. When the sample is pushed out, the pressing rod 4 is pushed down further while the first spring 5 is partially compressed. The head of the pressing rod 4 abuts against the sliding sleeve 7 and drives the sliding sleeve 7 to move downward. At this time, the second spring 6 is also compressed, which can drain the glutaraldehyde solution in the sampling cylinder 1 and the piston cavity 24. In other words, a small amount of glutaraldehyde can be absorbed by the compression and recovery of the first spring 5, while the glutaraldehyde solution in the sampling cylinder 1 and piston chamber 24 can be completely discharged by the compression of the second spring 6.

[0038] For easy assembly and disassembly, the pressing lever 4 includes a pressing handle 42, an upper section lever 43 detachably mounted on the pressing handle 42, and a lower section lever 44 detachably mounted on the upper section lever 43. A limiting lever 41 is mounted on the upper section lever 43. The upper section lever 43 and the lower section lever 44 can be connected by threads, and the upper section lever 43 can be inserted into the pressing handle 42.

[0039] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A suction-type tobacco leaf ultra-microstructure sampler, characterized in that, include: The sampling tube (1) has a sampling port (11) and a connection port (12), wherein the sampling port (11) has a blade; The air pump (2) has a lower port (21) and an upper port (22), the lower port (21) being connected to the connection port (12); Piston head (3) is installed inside the vacuum cylinder (2); The pressing rod (4) is installed on the upper end face of the piston head (3), and the upper end of the pressing rod (4) extends upward out of the upper port (22). The first spring (5) is fitted onto the outside of the pressing rod (4); In its natural state, under the action of the first spring (5), the pressing rod (4) drives the piston head (3) to move upward inside the air extraction cylinder (2).

2. The absorption-type tobacco leaf ultrastructure sampler according to claim 1, characterized in that, The vacuum pump (2) includes a spring chamber (23) and a piston chamber (24) installed at the bottom of the spring chamber (23). The diameter of the piston chamber (24) is smaller than the diameter of the spring chamber (23). The lower port (21) is the lower port (21) of the piston chamber (24), and the upper port (22) is the upper port (22) of the spring chamber (23). The piston head (3) is disposed in the piston cavity (24), and the pressing rod (4) is provided with a limiting rod (41). The first spring (5) is installed between the limiting rod (41) and the bottom end of the spring cavity (23).

3. The absorption-type tobacco leaf ultrastructure sampler according to claim 2, characterized in that, The upper port (22) of the spring cavity (23) is fitted with a cover (25), the cover (25) is provided with a through hole, and the limiting rod (41) is set on the rod of the pressing rod (4) below the cover (25); A sliding sleeve (7) is fitted onto the pressing rod (4) at a position on the cover (25). A second spring (6) is provided between the sliding sleeve (7) and the cover (25). The stiffness of the second spring (6) is greater than that of the first spring (5).

4. The absorption-type tobacco leaf ultrastructure sampler according to claim 2, characterized in that, The pressing rod (4) includes a pressing handle (42), an upper section rod (43) detachably mounted on the pressing handle (42), and a lower section rod (44) detachably mounted on the upper section rod (43). The limiting rod (41) is mounted on the upper section rod (43).

5. The absorption-type tobacco leaf ultrastructure sampler according to claim 1, characterized in that, The sampling tube (1) has a structure that is larger at the top and smaller at the bottom, and the sampling port (11) is a square opening.

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