Automatic enrichment and adsorption device for microorganisms

Abstract

The utility model discloses an automatic microorganism enrichment and adsorption device and relates to the technical field of biomedicine. The device comprises an enrichment adsorption box, the enrichment adsorption box is provided with a harmful bacterium adsorption assembly used for removing harmful bacteria in intestinal microorganism mixed liquid, a beneficial bacterium enrichment assembly used for enriching beneficial bacteria in the microorganism mixed liquid, a beneficial bacterium dissociation assembly used for dissociating the beneficial bacteria and antibody magnetic beads which are coupled together, and a beneficial bacterium collection bottle used for collecting the beneficial bacteria. And the negative pressure assembly is used for enabling the intestinal microorganism mixed liquid to flow through the harmful bacterium adsorption assembly, the beneficial bacterium enrichment assembly and the beneficial bacterium dissociation assembly and enter the beneficial bacterium collection bottle. According to the utility model, specific intestinal microorganisms can be selectively screened, so that a solid foundation is laid for precise transplantation of intestinal flora and precise treatment of specific related diseases of the intestinal tract.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, specifically to an automated microbial enrichment and adsorption device. Background Technology

[0002] Existing intestinal microbial isolation and collection equipment mainly involves filtering and removing impurities from feces to separate and collect intestinal microorganisms. There are two main approaches to existing intestinal microbial filtration and impurity removal devices: the first approach uses multi-stage filtration devices, as reported in domestic patents such as CN105624027B, CN105624024B, CN108676704B, and CN209052694U; the second approach uses nuclear pore membranes with different pore sizes, as reported in domestic patents such as CN108949631A and CN108676704A.

[0003] Due to the high diversity and complexity of fecal samples, the substances obtained by the existing intestinal microbial isolation and collection equipment are all mixed intestinal microbial solutions (including beneficial bacteria, harmful bacteria, fungi, viruses and other microorganisms). They cannot selectively screen specific intestinal microorganisms (such as beneficial bacteria), and are difficult to meet the future needs for precise transplantation of intestinal flora and precise treatment of specific intestinal-related diseases. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide an automatic microbial enrichment and adsorption device to achieve selective screening of specific intestinal microorganisms.

[0005] To achieve the above objectives, the technical solution designed by this utility model is as follows:

[0006] An automated microbial enrichment and adsorption device includes a negative pressure component, a beneficial bacteria enrichment component for positive enrichment of beneficial bacteria in a microbial mixture, a beneficial bacteria dissociation component for dissociation of beneficial bacteria couplers, and a beneficial bacteria collection bottle for collecting beneficial bacteria. The negative pressure component allows the microbial mixture to flow sequentially through the beneficial bacteria enrichment component and the beneficial bacteria dissociation component before entering the beneficial bacteria collection bottle.

[0007] In the above technical solution, the beneficial bacteria enrichment component includes a second antibody magnetic bead, a beneficial bacteria magnetic bead container, and a beneficial bacteria enrichment bottle. The beneficial bacteria magnetic bead container is provided with a beneficial bacteria coupling element for coupling beneficial bacteria and the second antibody magnetic beads together. The beneficial bacteria enrichment bottle is provided with a beneficial bacteria enrichment element for positive enrichment of the beneficial bacteria coupler.

[0008] In the above technical solution, the beneficial bacteria enrichment component also includes a waste liquid collection bottle. The beneficial bacteria enrichment bottle is provided with a waste liquid inlet. The waste liquid collection bottle is connected to the waste liquid inlet to collect the waste liquid after the forward enrichment treatment.

[0009] In the above technical solution, the beneficial bacteria coupling component includes a second stirring motor and a second material inlet. The second material inlet is used to introduce second antibody magnetic beads into the beneficial bacteria magnetic bead container. The output end of the second stirring motor is connected to a second stirring blade located inside the beneficial bacteria magnetic bead container.

[0010] In the above technical solution, the beneficial bacteria enrichment device includes a second electromagnetic induction coil and a second coil controller. The second electromagnetic induction coil is sleeved on the beneficial bacteria enrichment bottle. The second coil controller and the second electromagnetic induction coil are electrically connected to control the working state of the second electromagnetic induction coil.

[0011] In the above technical solution, the beneficial bacteria dissociation component includes a beneficial bacteria dissociation tank and a magnetic bead adsorption bottle. The beneficial bacteria dissociation tank is provided with a beneficial bacteria dissociation element for dissociating the beneficial bacteria coupler, and the magnetic bead adsorption bottle is provided with a magnetic bead adsorption element for adsorbing the second antibody magnetic beads.

[0012] In the above technical solution, the beneficial bacteria dissociation device includes a third stirring motor and a third material inlet. The third material inlet is used to introduce dissociation enzymes into the beneficial bacteria dissociation tank. The output end of the third stirring motor is connected to a third stirring blade located inside the beneficial bacteria dissociation tank.

[0013] In the above technical solution, the magnetic bead adsorption component includes a third electromagnetic induction coil and a third coil controller. The third electromagnetic induction coil is sleeved on the magnetic bead adsorption bottle. The third coil controller and the third electromagnetic induction coil are electrically connected to control the working state of the third electromagnetic induction coil.

[0014] In the above technical solution, this application also includes a harmful bacteria adsorption component for removing harmful bacteria from the microbial mixture. The negative pressure component is used to allow the microbial mixture to flow sequentially through the harmful bacteria adsorption component, the beneficial bacteria enrichment component, and the beneficial bacteria dissociation component before entering the beneficial bacteria collection bottle.

[0015] In the above technical solution, the harmful bacteria adsorption component includes a first antibody magnetic bead, a harmful bacteria magnetic bead container, and a harmful bacteria adsorption bottle. The harmful bacteria magnetic bead container is provided with a harmful bacteria coupling element for coupling the harmful bacteria and the first antibody magnetic beads together. The harmful bacteria adsorption bottle is provided with a harmful bacteria adsorption element for reverse enrichment of the harmful bacteria coupler.

[0016] In the above technical solution, the harmful bacteria coupling component includes a first stirring motor and a first material inlet. The first material inlet is used to introduce first antibody magnetic beads into the harmful bacteria magnetic bead tank. The output end of the first stirring motor is located in the first stirring blade inside the harmful bacteria magnetic bead tank.

[0017] In the above technical solution, the harmful bacteria adsorption device includes a first electromagnetic induction coil and a first coil controller. The first electromagnetic induction coil is sleeved on the harmful bacteria adsorption bottle. The first coil controller and the first electromagnetic induction coil are electrically connected to control the working state of the first electromagnetic induction coil.

[0018] In the above technical solution, this application also includes an enrichment adsorption box, which is further provided with a first partition and a second partition. The first partition divides the interior of the enrichment adsorption box into an operation area and a background area, and the second partition divides the operation area into a clean area and a contaminated area. The harmful bacteria adsorption component, the beneficial bacteria enrichment component, and the beneficial bacteria dissociation component are all located in the contaminated area, the beneficial bacteria collection bottle is located in the clean area, and the negative pressure component is installed on the first partition.

[0019] In the above technical solution, the negative pressure component includes a first peristaltic pump installed between the harmful bacteria adsorption component and the beneficial bacteria enrichment component, a second peristaltic pump installed between the beneficial bacteria enrichment component and the beneficial bacteria dissociation component, and a third peristaltic pump installed between the beneficial bacteria dissociation component and the beneficial bacteria collection bottle.

[0020] In the above technical solution, this application also includes an odor purification bottle and a negative pressure pump. One end of the negative pressure pump is connected in sequence to the odor purification bottle and the harmful bacteria adsorption component, and the other end is connected in sequence to the buffer, the filter and the beneficial bacteria collection bottle.

[0021] In the above technical solution, the buffer includes a first buffer bottle and a second buffer bottle arranged sequentially along the gas flow direction.

[0022] In the above technical solution, a display controller is also provided on the outer shell of the clean area, and the harmful bacteria adsorption component, the beneficial bacteria enrichment component and the beneficial bacteria dissociation component are all electrically connected to the display controller.

[0023] In the above technical solution, the lower end of the enrichment adsorption box is provided with a nitrogen cabinet for holding nitrogen and a consumable cabinet for holding consumables, and the nitrogen cabinet is located between the enrichment adsorption box and the consumable cabinet.

[0024] In the above technical solution, the enrichment adsorption box is also equipped with one or more of the following: printer, fan, buzzer, spotlight and lighting.

[0025] The beneficial effects of this utility model are:

[0026] 1. Improved purity and safety of bacterial solution: This application can further process the microbial mixture obtained from the donor (such as fecal sample). With the joint work of the beneficial bacteria enrichment component and the beneficial bacteria dissociation component, this application can extract (enrich) beneficial bacteria from the microbial mixture, providing a safe and reliable source of bacteria for subsequent microbial transplantation applications.

[0027] 2. Removal of trace amounts of harmful bacteria that may be present in the donor: When the intestinal microbial mixture (including beneficial bacteria, harmful bacteria, fungi, viruses and other microorganisms) obtained by existing intestinal microbial isolation and collection equipment is applied to intestinal microbiota transplantation (FMT) technology, it generally relies on algorithms to achieve a certain degree of matching. The process adjustment of the microbial isolation and purification process is very limited. The effect of microbiota transplantation is severely limited by the donor's own microbial structure. The potential trace amounts of undetected harmful pathogens also bring huge clinical risks, making it difficult to meet the needs of precise treatment of diseases by intestinal microbiota.

[0028] With the help of the harmful bacteria adsorption component, this application can efficiently remove harmful bacteria from the microbial mixture, solve the problem of potential harmful bacteria retention in traditional physical screening technology, further improve the purity of the bacterial solution, and provide a safer and more reliable source of bacteria for subsequent microbial transplantation applications.

[0029] 3. High working efficiency and convenient to use: Under the action of the negative pressure component, this application can automatically extract (enrich) beneficial bacteria from the microbial mixture, which has high working efficiency and is convenient to use.

[0030] 4. Precise Enrichment and Standardized Operation: This patent employs a precise enrichment technology for beneficial bacteria. Through a screening mechanism based on highly specific antibody-protein binding, it achieves the rapid and accurate separation and enrichment of target beneficial bacteria. Furthermore, this patent implements a standardized operating procedure, enabling the production of bacterial solutions with stable quality and consistent performance, greatly improving the reproducibility of the bacterial solutions.

[0031] 5. Improve the activity and stability of the bacterial community: Since many beneficial bacteria can only maintain their optimal activity and stability under low-oxygen or anaerobic conditions, this patented device introduces a low-oxygen device (nitrogen cylinder related components), which can create a relatively stable anaerobic environment during the bacterial solution treatment process by precisely controlling the oxygen content, thereby significantly improving the activity of the bacterial community, extending the shelf life of the bacterial solution, and further improving the quality of the bacterial solution. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the structure of the automated microbial enrichment and adsorption device described in this application;

[0033] Figure 2A schematic diagram of the enrichment adsorption box without the first and second cover plates;

[0034] Figure 3 for Figure 2 The main view;

[0035] Figure 4 for Figure 2 Rear view;

[0036] Figure 5 This is a flowchart illustrating the process of this application.

[0037] In the diagram: 200, enrichment and adsorption device; 201, printer; 202, fan; 203, buzzer; 204, spotlight; 205, lighting; 206, first cover plate; 207, second cover plate; 208, enrichment and adsorption box; 210, harmful bacteria adsorption assembly; 211, first antibody magnetic bead; 212, harmful bacteria magnetic bead container; 213, harmful bacteria adsorption bottle; 214, harmful bacteria coupling component; 2141, first stirring motor; 2142, first material inlet; 2143, first stirring shaft; 2144 215. First stirring blade; 2156. Harmful bacteria adsorption component; 2151. First electromagnetic induction coil; 2152. First coil controller; 220. Beneficial bacteria enrichment component; 221. Second antibody magnetic beads; 222. Beneficial bacteria magnetic bead container; 223. Beneficial bacteria enrichment bottle; 224. Beneficial bacteria coupling component; 2241. Second stirring motor; 2242. Second material inlet; 2243. Second stirring shaft; 2244. Second stirring blade; 225. Beneficial bacteria enrichment component; 2251. Second electromagnetic induction coil; 22 52. Second coil controller; 226. Waste liquid collection bottle; 230. Beneficial bacteria dissociation assembly; 232. Beneficial bacteria dissociation tank; 233. Magnetic bead adsorption bottle; 234. Beneficial bacteria dissociation component; 2341. Third stirring motor; 2342. Third material inlet; 2343. Third stirring shaft; 2344. Third stirring blade; 235. Magnetic bead adsorption component; 2351. Third electromagnetic induction coil; 2352. Third coil controller; 240. Beneficial bacteria collection bottle; 250. Negative pressure assembly; 251. First worm 252. Peristaltic pump; 253. Peristaltic pump; 261. First partition; 262. Second partition; 263. Operating area; 264. Back-office area; 265. Clean area; 266. Contaminated area; 271. Odor purification bottle; 272. Negative pressure pump; 273. Buffer; 2731. First buffer bottle; 2372. Second buffer bottle; 274. Filter; 275. First control valve; 276. Second control valve; 280. Display controller; 291. Nitrogen cabinet; 292. Consumables cabinet. Detailed Implementation

[0038] The present invention will now be described in further detail with reference to specific embodiments, so that those skilled in the art can understand it.

[0039] The interaction between humans and microorganisms is extremely close. Shortly after birth, a large number of external microorganisms begin to attach to the surface of a newborn's body and intestines. Most of these microorganisms adapt to the human body, eventually forming a symbiotic relationship that lasts a lifetime. Of these symbiotic microorganisms, approximately 100 trillion colonize the human gut, encompassing bacteria, fungi, viruses, and many other microorganisms. The gut microbiota is the most complex and largest micro-ecosystem in the human body, often referred to as the eighth organ. The genome of the gut microbiota contains approximately 5 million genes, about 150 times the number of genes in the human body. Due to its vast quantity and rich diversity, the gut microbiota is known as the human body's "second gene pool."

[0040] Gut microbiota can produce short-chain fatty acids (SCFAs) by fermenting dietary fiber that is difficult for humans to digest. These SCFAs provide energy for colonic epithelial cells, regulate the interaction between innate and adaptive immunity, and support the host's defense mechanisms against intestinal pathogens. A normal and stable gut microbiota plays a crucial role in the body's defense against infection, maintaining a normal intestinal barrier, and supporting immunity, metabolism, nutrition, and homeostasis through interactions with multiple systems, including the immune, endocrine, and nervous systems.

[0041] Immunomagnetic bead enrichment and separation technology is a routine method in this field for separating target molecules or cells based on the principle of specific antigen-antibody binding. This technology utilizes magnetic bead microspheres composed of core metal particles made of ferric oxide (Fe3O4) or ferric oxide (Fe2O4), polymeric materials, and functional ligands. The chemical groups on the surface of the microspheres can covalently couple with antibodies to form stable and robust magnetic bead-antibody conjugates. Antibodies can specifically bind to target proteins or antigens on the cell surface through an immune response, forming microbial-magnetic bead-antibody complexes. The ferric oxide gives the magnetic beads superparamagnetism; the microspheres are magnetized and aggregate in a magnetic field environment, and lose magnetism and disperse when removed from the magnetic field. Under the influence of an external magnetic field, immunomagnetic beads can rapidly aggregate, facilitating liquid separation.

[0042] Therefore, immunomagnetic bead separation technology is often used in cell separation, immunoassay, molecular biology detection and microbiology detection due to its advantages of high specificity, simple operation and high purity.

[0043] Currently, fecal microbiota transplantation (FMT) technology is gradually gaining widespread attention. In 2023, the National Health Commission officially included FMT in its "Notice on Issuing the National Technical Specifications for Medical Service Projects." As an innovative treatment method, FMT can provide a new approach for the treatment of various diseases by restoring the balance of the gut microbiota.

[0044] Fecal microbiota transplantation (FMT) originated from the Eastern Jin Dynasty book "Elbow-Side Emergency Prescriptions" and the Spring and Autumn and Warring States period book "Suwen". Traditional Chinese medicine used human feces (recorded as Huanglong Decoction / Golden Juice) to treat intestinal diseases. In 1958, surgeon Eiseman used human fecal enema to treat patients with pseudomembranous colitis. In 2013, the American Medical Association announced that human feces can be used for the treatment of Clostridium difficile.

[0045] Subsequently, gut microbiota transplantation technology has shown significant efficacy in treating gastrointestinal diseases such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), recurrent Clostridium difficile infection (CDI), ulcerative colitis (UC), and functional constipation (FC). With in-depth research and continuous updates in technologies such as high-throughput sequencing, artificial intelligence big data analysis, and intelligent production systems, its clinical application cases are also increasing, showing broad application prospects.

[0046] Currently, isolating gut microbiota (including gut flora) from feces is a huge challenge for medical staff. How to achieve the isolation and collection of gut microbiota is of great significance to medical staff, as it can lay a solid foundation for precise gut flora transplantation and precision treatment of gut-related diseases.

[0047] Existing gut microbiota separation and collection devices mainly filter and remove impurities from feces to separate and collect gut microbiota. They cannot selectively screen specific gut microbiota (such as beneficial bacteria), making it difficult to meet the future needs for precise gut microbiota transplantation and precise treatment of specific gut-related diseases.

[0048] like Figure 1 As shown, this utility model provides an automatic microbial enrichment and adsorption device, which includes an enrichment and adsorption box 208; the enrichment and adsorption box 208 is provided with a harmful bacteria adsorption component 210 for removing harmful bacteria from the intestinal microbial mixture, a beneficial bacteria enrichment component 220 for enriching beneficial bacteria from the microbial mixture, a beneficial bacteria dissociation component 230 for dissociating the coupled beneficial bacteria and antibody magnetic beads, a beneficial bacteria collection bottle 240 for collecting beneficial bacteria, and a negative pressure component 250. The negative pressure component 250 is used to allow the intestinal microbial mixture to flow sequentially through the harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220 and the beneficial bacteria dissociation component 230, and enter the beneficial bacteria collection bottle 240.

[0049] The automated microbial enrichment and adsorption device of this application, also known as the enrichment and adsorption device 200, achieves more efficient selective removal of harmful bacteria and enrichment of beneficial bacteria in intestinal microbiota transplantation technology, thereby realizing precise transplantation. This application greatly improves the sorting efficiency of target probiotics, especially the precise capture of low-abundance strains. This makes the fecal microbiota separation and screening work for precise transplantation more efficient and specific, thereby promoting improved production efficiency. This application can play a more important role in the field of intestinal microbiota transplantation, achieving precise removal of harmful bacteria and precise enrichment of beneficial bacterial flora, and will become a revolutionary technology leading standardized intestinal microbiota preparation.

[0050] This application primarily involves first enriching the collected microbial mixture to remove harmful bacteria and enrich beneficial bacteria, thereby obtaining a beneficial bacteria coupler (second antibody magnetic beads 221 + beneficial bacteria); then dissociating the beneficial bacteria coupler to separate the beneficial bacteria from the antibody magnetic beads, thus obtaining the beneficial bacteria. Specifically:

[0051] This application first conjugates an antibody that identifies harmful bacteria to a magnetic bead to obtain a first antibody magnetic bead 211 (also known as a harmful bacteria antibody magnetic bead). Then, it performs reverse enrichment using a magnetic field to adsorb harmful bacteria in the intestinal microbial mixture. Next, the remaining intestinal microbial mixture is enriched with beneficial bacteria from fecal bacteria according to the same principle. Then, a dissociation enzyme is used to dissociate the coupled beneficial bacteria and the second antibody magnetic bead 221, and finally, the target beneficial bacteria are obtained from the fecal bacteria.

[0052] It should be noted that the harmful bacteria adsorption component 210 is a preferred embodiment in this application. The harmful bacteria adsorption component 210 can efficiently remove harmful bacteria from the microbial mixture, solve the problem of potential harmful bacteria retention in traditional physical screening technologies, further improve the purity of the bacterial solution, and provide a safer and more reliable source of bacteria for subsequent microbial transplantation applications.

[0053] like Figure 1 As shown, the enrichment adsorption box 208 is also provided with a first partition 261 and a second partition 262. The first partition 261 divides the interior of the enrichment adsorption box 208 into an operation area 263 and a background area 264. The second partition 262 divides the operation area 263 into a clean area 265 and a contaminated area 266. The harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220 and the beneficial bacteria dissociation component 230 are all located in the contaminated area 266. The beneficial bacteria collection bottle 240 is located in the clean area 265. The negative pressure component 250 is installed on the first partition 261.

[0054] In actual operation, the back-end area 264 is mainly used to place components that do not require operator intervention (and are frequently replaced), such as the negative pressure pump 272, the first buffer bottle 2731, and the second buffer bottle 2372; the operating area 263 is mainly used to place components that require operator intervention and are frequently replaced, such as the harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220, the beneficial bacteria dissociation component 230, and the beneficial bacteria collection bottle 240. Preferably, the first partition 261, the second partition 262, and the enrichment and adsorption box 208 are provided with multiple through holes to allow pipelines to pass through, thereby making the use of this application more convenient.

[0055] By dividing the interior of the enrichment adsorption box 208 into an operation area 263, a back-end area 264, a contaminated area 266, and a clean area 265, this application can avoid cross-contamination, thus making it more convenient to use. When it is necessary to replace sampling consumables (such as the harmful bacteria magnetic bead container 212, the beneficial bacteria magnetic bead container 222, and the beneficial bacteria dissociation container, etc.), people only need to open the first cover 206; when it is necessary to replenish or replace the beneficial bacteria collection bottle 240 or the waste liquid collection bottle 226, this application only needs to open the second cover 207. In this way, the contaminated area 266, the clean area 265, and the back-end area 264 cooperate with each other without interfering with each other, which can avoid cross-contamination, thus making this application cleaner, more hygienic, and more convenient to use.

[0056] like Figure 1 and Figure 2 As shown, this application forms a slope (60-85°, preferably 75°) on the front end face of the enrichment adsorption box 208, so that the staff can easily check the working status of the harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220 and the magnetic bead dissociation and collection component through the glass on the first cover plate 206, and increase the aesthetics of this application.

[0057] The clean area 265 is also equipped with a display controller 280. The harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220 and the beneficial bacteria dissociation component 230 are all electrically connected to the display controller 280.

[0058] The display controller 280 can be used to control the working status of components such as the harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220, and the beneficial bacteria dissociation component 230. The real-time working parameters of the harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220, and the beneficial bacteria dissociation component 230 can be directly displayed on the display controller 280, so that the staff can adjust the working status of the harmful bacteria adsorption component 210, the beneficial bacteria enrichment component 220, and the beneficial bacteria dissociation component 230 in real time.

[0059] The lower end of the enrichment adsorption box 208 is connected to a nitrogen cabinet 291 and a consumable cabinet 292. The nitrogen cabinet 291 is equipped with a nitrogen cylinder 292 for filling the enrichment adsorption box 208 with nitrogen. The enrichment adsorption box 208 is equipped with a nitrogen switch valve connected to the nitrogen cylinder 292 and used to control the oxygen concentration in the enrichment adsorption box 208.

[0060] With the help of the nitrogen cylinder, this application can keep the enrichment adsorption box 208 in a low-oxygen state, thereby improving the survival rate and activity of anaerobic bacteria in feces. The enrichment adsorption box 208 can also be equipped with components such as an oxygen sensor. The oxygen sensor can be used to detect the oxygen concentration in the enrichment adsorption box 208. The display controller 280 can control the opening or closing of the nitrogen switch valve according to the oxygen concentration fed back by the oxygen sensor, thereby controlling the oxygen concentration in the enrichment adsorption box 208.

[0061] This application ensures the activity and stability of the bacterial community to a greater extent during the separation process. By adding a low-oxygen device (nitrogen cylinder), it provides an anaerobic environment with a relatively low oxygen concentration, which is more conducive to maintaining the abundance of beneficial bacteria and improving the activity of the bacterial community. This application will play a more important role in the field of intestinal microbiota transplantation, achieving precise removal of harmful bacteria and precise enrichment of beneficial bacterial communities, and will lead to technological innovation in standardized intestinal microbiota preparation.

[0062] The enrichment adsorption chamber 208 is also equipped with one or more of the following: a printer 201, a fan 202, a buzzer 203, a spotlight 204, and a light 205. The printer 201 can be used to print test records, the fan 202 can be used to reduce the oxygen concentration in the contaminated area 266, the buzzer 203 can be used for alarm prompts, the light 205 can be used to illuminate the contaminated area 266 and the clean area 265, and the spotlight 204 can be used to centrally reflect the working status of the contaminated area 266, thereby making this application more convenient to use.

[0063] In actual operation, the printer 201, lighting 205, spotlight 204, socket, control cabinet, fan 202 and buzzer 203 mentioned above are all conventional products in this field, and their installation methods and installation positions are also conventional methods in this field, which will not be described in detail here.

[0064] like Figure 2 and Figure 3 As shown, the harmful bacteria adsorption assembly 210 includes a first antibody magnetic bead 211, a harmful bacteria magnetic bead container 212, and a harmful bacteria adsorption bottle 213. The harmful bacteria magnetic bead container 212 is provided with a harmful bacteria coupling element 214 for coupling the harmful bacteria and the first antibody magnetic bead 211 together. The harmful bacteria adsorption bottle 213 is provided with a harmful bacteria adsorption element 215 for reverse enrichment of the harmful bacteria coupler (i.e., the coupled harmful bacteria and the first antibody magnetic bead 211).

[0065] The main function of the harmful bacteria adsorption component 210 is to remove (extremely small amounts) of harmful bacteria that may be present in the intestinal microbial mixture, thereby reducing competition and potential dangers from harmful bacteria and improving the safety of the bacterial solution. Preferably, this step can remove multiple harmful bacteria simultaneously to further improve the safety of the bacterial solution.

[0066] The harmful bacteria coupling component 214 includes a first stirring motor 2141 and a first material inlet 2142. The first material inlet 2142 is used to introduce first antibody magnetic beads 211 into the harmful bacteria magnetic bead container 212. The output end of the first stirring motor 2141 is connected to a first stirring shaft 2143, and a first stirring blade 2144 located inside the harmful bacteria magnetic bead container 212 is connected inside the first stirring shaft 2143. Under the combined action of the first stirring motor 2141 and the first stirring blade 2144, the first antibody magnetic beads and harmful bacteria will couple inside the harmful bacteria magnetic bead container 212, thereby obtaining a harmful bacteria coupling body.

[0067] The harmful bacteria adsorption component 215 includes a first electromagnetic induction coil 2151 and a first coil controller 2152. The first electromagnetic induction coil 2151 is sleeved on the harmful bacteria adsorption bottle 213. The first coil controller 2152 is electrically connected to the first electromagnetic induction coil 2151 to control the working state of the first electromagnetic induction coil 2151.

[0068] The first coil controller 2152 can energize or de-energize the first electromagnetic induction coil 2151. When the first electromagnetic induction coil 2151 is energized, it generates a magnetic field, which adsorbs the harmful bacteria coupler, thereby achieving the purpose of removing harmful bacteria from the intestinal microorganisms.

[0069] The beneficial bacteria enrichment component 220 includes a second antibody magnetic bead 221, a beneficial bacteria magnetic bead container 222, a beneficial bacteria enrichment bottle 223, and a waste liquid collection bottle 226. The harmful bacteria magnetic bead container 212 is provided with a beneficial bacteria coupling element 224 for coupling beneficial bacteria and the second antibody magnetic bead 221 together. The beneficial bacteria enrichment bottle 223 is provided with a beneficial bacteria enrichment element 225 for positive enrichment of the beneficial bacteria couple (i.e., the coupled beneficial bacteria and the second antibody magnetic bead 221).

[0070] Furthermore, the beneficial bacteria enrichment bottle 223 is equipped with a waste liquid inlet, and the waste liquid collection bottle 226 is connected to the waste liquid inlet to collect the waste liquid after the forward enrichment treatment. This makes the application more convenient to use.

[0071] The main function of the beneficial bacteria enrichment component 220 is to automatically enrich the (extremely small amount) of beneficial bacteria that may be present in the intestinal microbial mixture. Preferably, this step can enrich multiple beneficial bacteria at the same time to further improve the enrichment efficiency of beneficial bacteria.

[0072] The beneficial bacteria coupling component 224 includes a second stirring motor 2241 and a second material inlet 2242. The second material inlet 2242 is used to introduce second antibody magnetic beads 221 into the beneficial bacteria magnetic bead container 222. The output end of the second stirring motor 2241 is connected to a second stirring shaft 2243, and a second stirring blade 2244 located inside the beneficial bacteria magnetic bead container 222 is connected to the second stirring shaft 2243. Under the combined action of the second stirring motor 2241 and the second stirring blade 2244, the second antibody magnetic beads 221 and beneficial bacteria will couple within the beneficial bacteria magnetic bead container 222, thereby obtaining a beneficial bacteria coupling body.

[0073] The beneficial bacteria enrichment device 225 includes a second electromagnetic induction coil 2251 and a second coil controller 2252. The second electromagnetic induction coil 2251 is sleeved on the beneficial bacteria enrichment bottle 223. The second coil controller 2252 and the second electromagnetic induction coil 2251 are electrically connected to control the working state of the second electromagnetic induction coil 2251.

[0074] The second coil controller 2252 can energize or de-energize the second electromagnetic induction coil 2251. When the second electromagnetic induction coil 2251 is energized, it generates a magnetic field, which adsorbs the beneficial bacteria coupler, thereby enabling the present application to achieve the purpose of enriching beneficial bacteria in intestinal microorganisms.

[0075] The beneficial bacteria dissociation assembly 230 includes a beneficial bacteria dissociation tank 232 and a magnetic bead adsorption bottle 233. The beneficial bacteria dissociation tank 232 is provided with a beneficial bacteria dissociation element 234 for dissociating the coupled beneficial bacteria and the second antibody magnetic beads 221. The magnetic bead adsorption bottle 233 is provided with a magnetic bead adsorption element 235 for adsorbing the second antibody magnetic beads 221.

[0076] The main function of the beneficial bacteria dissociation component 230 is to dissociate the coupled beneficial bacteria and the second antibody magnetic beads 221 so as to facilitate the collection of beneficial bacteria.

[0077] The beneficial bacteria dissociation device 234 includes a third stirring motor 2341 and a third material inlet 2342. The third material inlet 2342 is used to introduce dissociation enzymes into the beneficial bacteria dissociation tank 232. The output end of the third stirring motor 2341 is connected to a third stirring shaft 2343, and a third stirring blade 2344 located inside the beneficial bacteria dissociation tank 232 is connected to the third stirring shaft 2343. Under the combined action of the third stirring motor 2341, the third stirring blade 2344, and the dissociation enzyme, the coupled beneficial bacteria and the second antibody magnetic beads 221 will dissociate, thereby achieving the purpose of collecting beneficial bacteria.

[0078] The magnetic bead adsorption component 235 includes a third electromagnetic induction coil 2351 and a third coil controller 2352. The third electromagnetic induction coil 2351 is sleeved on the magnetic bead adsorption bottle 233. The third coil controller 2352 and the third electromagnetic induction coil 2351 are electrically connected to control the working state of the third electromagnetic induction coil 2351.

[0079] The third coil controller 2352 can energize or de-energize the third electromagnetic induction coil 2351. When the third electromagnetic induction coil 2351 is energized, it generates a magnetic field, which adsorbs the second antibody magnetic beads 221 to remove the second antibody magnetic beads 221 from the mixture, thereby allowing the present application to collect beneficial bacteria with higher purity.

[0080] In actual operation, the first electromagnetic induction coil 2151, the second electromagnetic induction coil 2251, and the third electromagnetic induction coil 2351 can be collectively referred to as electromagnetic induction coils. The first coil controller 2152, the second coil controller 2252, and the third coil controller 2352 can all be integrated on the display controller 280 and can be collectively referred to as coil controllers. Controlling the operating state (energized or de-energized state) of each connected electromagnetic induction coil through the coil controller is a conventional method in this field and will not be elaborated here. Preferably, the coil controller can also control the current intensity flowing through the electromagnetic induction coils to control the magnetic field strength of each connected electromagnetic induction coil.

[0081] The negative pressure assembly 250 includes a first peristaltic pump 251 installed between the harmful bacteria adsorption assembly 210 and the beneficial bacteria enrichment assembly 220, a second peristaltic pump 252 installed between the beneficial bacteria enrichment assembly 220 and the beneficial bacteria dissociation assembly 230, and a third peristaltic pump 253 installed between the beneficial bacteria dissociation assembly 230 and the beneficial bacteria collection bottle 240.

[0082] In actual operation, the first peristaltic pump 251, the second peristaltic pump 252, and the third peristaltic pump 253 can all be installed within the contaminated area 266 (e.g., Figure 2 and Figure 3 As shown, the first peristaltic pump 251, the second peristaltic pump 252, and the third peristaltic pump 253 are all electrically connected to the display controller 280. The harmful bacteria magnetic bead tank 212, the first peristaltic pump 251, the harmful bacteria adsorption bottle 213, the beneficial bacteria magnetic bead tank 222, the second peristaltic pump 252, the beneficial bacteria enrichment bottle 223, the beneficial bacteria dissociation tank 232, the third peristaltic pump 253, the magnetic bead adsorption bottle 233, and the beneficial bacteria collection bottle 240 are connected in sequence through pipelines.

[0083] This application also includes an odor purification bottle 271 and a negative pressure pump 272. One end of the negative pressure pump 272 is connected to the odor purification bottle 271 and the harmful bacteria adsorption component 210 in sequence, and the other end is connected to the buffer 273, the filter 274 and the beneficial bacteria collection bottle 240 in sequence.

[0084] In actual operation, the negative pressure pump 272 can be a negative pressure vacuum pump. One end of the negative pressure pump 272 is connected to the odor purification bottle 271 and the harmful bacteria magnetic bead container 212 in sequence through pipelines, and the other end is connected to the buffer 273, the filter 274 and the beneficial bacteria collection bottle 240 in sequence through pipelines.

[0085] The buffer 273 includes a first buffer bottle 2731 and a second buffer bottle 2372 arranged sequentially along the gas flow direction. A first control valve 275 is provided between the odor purification bottle 271 and the odor purification bottle 272, and a second control valve 276 is provided between the filter 274 and the beneficial bacteria collection bottle 240. In actual operation, both the first buffer bottle 2731 and the second buffer bottle 2372 can be pneumatic diaphragm pump buffer bottles, and both the first control valve 275 and the second control valve 276 can be pneumatic control valves.

[0086] refer to Figure 5 The method for enriching and adsorbing gut microbiota in this application includes:

[0087] K1. The microbial mixture is delivered to the harmful bacteria magnetic bead tank 212 through a pipeline. The first antibody magnetic bead 211 enters the harmful bacteria magnetic bead tank 212 through the first material port 2142. The first stirring motor 2141 and its connected first stirring blade 2144 rotate and mix for 10 minutes. Then, it is incubated at room temperature for 40 minutes to allow the harmful bacteria antibody magnetic beads to couple with the harmful bacteria and obtain a harmful bacteria couple body.

[0088] This application couples harmful bacteria magnetic beads that have undergone surface carboxyl group activation treatment with corresponding antibodies of the harmful bacteria to obtain first antibody magnetic beads 211. These first antibody magnetic beads 211 are prepared before being sent to the harmful bacteria magnetic bead container 212 to avoid affecting the harmful bacteria coupling obtained in this application. This application uses Salmonella as an example to further explain how this application achieves the adsorption of harmful bacteria, making the technical solution of this invention easier to understand:

[0089] K11, Carboxyl group activation on the surface of harmful bacteria magnetic beads

[0090] In a clean bench, take 1000 mL of L Ag COOH sterile magnetic bead suspension (product number 70113-5, Suzhou Beaver Biotechnology) (magnetic bead concentration 5 mg / mL) and transfer it to a 5000 mL sterile centrifuge bottle. Magnetic separation is performed to remove the supernatant. The product is then magnetically washed twice with 2000 mL of MEST solution (100 mM MES, pH 5.0, 0.05% Tween 20), and the supernatant is removed. Freshly prepared 1000 mL of EDC solution (10 mg / mL, using the above MEST solution as a dispersant) and 1000 mL of NHS solution (10 mg / mL, using the above MEST solution as a dispersant) are added to the centrifuge tube containing the magnetic beads. Vortex to mix and fully suspend the magnetic beads. Activate at room temperature for 30 min.

[0091] Transfer the magnetic bead suspension to new sterile centrifuge tubes at 100 mL per tube. Maintain the magnetic beads in suspension during this process (this can be achieved by inverting the tubes using a vertical mixer). After these steps, the carboxyl groups on the surface of the magnetic beads will be activated, allowing for covalent coupling with biological ligands containing primary amino groups. (The activated state should not be stored for extended periods; immediate coupling is recommended).

[0092] Covalent coupling of K12, magnetic beads, and harmful bacteria antibodies

[0093] Replace the Salmonella monoclonal antibody buffer with 15 mM MES buffer (pH = 6.0) and dilute the antibody to 5 mg / mL with MES buffer. Take 4 mL of the antibody and mix thoroughly with 100 mL of the activated carboxyl magnetic beads (10 μm in diameter). Incubate at room temperature for 2 h, keeping the magnetic beads in suspension during conjugation (this can be achieved by inverting the beads using a vertical mixer). Perform magnetic separation, aspirate the supernatant, and simultaneously detect the remaining antibody content in the supernatant to calculate the amount and concentration of antibody conjugated to the magnetic beads. Wash the magnetic beads 2 to 3 more times and resuspend them in physiological saline to obtain Salmonella monoclonal antibody magnetic beads. These Salmonella monoclonal antibody magnetic beads can accurately enrich Salmonella.

[0094] In practice, staff can replace Salmonella with other harmful bacteria, such as Salmonella enterica and Clostridium difficile. Simultaneously, during step K12, this application can place multiple harmful bacteria antibody beads (i.e., first antibody beads 211) into the harmful bacteria magnetic bead container 212 to simultaneously remove multiple harmful bacteria from the intestinal microbial mixture.

[0095] Furthermore, in order to improve work efficiency, the harmful bacteria in this application may be one or more of the following: Salmonella, Shigella, Campylobacter jejuni, Campylobacter uppsala, Campylobacter coli, Clostridium difficile, Vibrio parahaemolyticus, Vibrio cholerae, Vibrio vulnificus, Yersinia enterocolitica, pathogenic Escherichia coli (EPEC), Shiga toxin-producing Escherichia coli (STEC), Escherichia coli O157, Shiga-like spp., Helicobacter pylori, Staphylococcus aureus, Enterobacter cloacae, and Cronobacter sakazakii.

[0096] K13, the first bacterial antibody magnetic beads are coupled with harmful bacteria to obtain harmful bacteria couplers.

[0097] The mixture after filtration enters the harmful bacteria magnetic bead tank 212 through the filtrate inlet pipe. The aforementioned harmful bacteria antibody magnetic beads are taken and mixed in a certain ratio (e.g., 1:1) to obtain the first antibody magnetic beads 211. The first antibody magnetic beads 211 are added to the harmful bacteria magnetic bead tank 212 through the first material inlet 2142. The first antibody magnetic beads 211 and the intestinal microbial mixture are mixed, and the blades connected to the first stirring motor 2141 are rotated to mix for 10 minutes, followed by static incubation for 40 minutes. This allows the first antibody magnetic beads 211 to bind with harmful bacteria (e.g., Salmonella) to obtain harmful bacteria couplers.

[0098] In actual work, the first antibody magnetic bead 211 can also be called a harmful bacteria antibody magnetic bead. The methods for obtaining the first antibody magnetic bead 211 and the methods for coupling the first antibody magnetic bead 211 with harmful bacteria to form a harmful bacteria couple body are all conventional methods in the field and will not be described in detail here.

[0099] K2, the negative pressure pipeline carries the harmful bacteria coupler into the harmful bacteria adsorption bottle 213, and under the action of the first electromagnetic induction coil 2151, the harmful bacteria coupler is reverse enriched, thereby removing the harmful bacteria in the microbial mixture.

[0100] In practice, this step aims to remove trace amounts of harmful bacteria that may be present in the donor, reducing competition and potential dangers from harmful bacteria, and ensuring the safety of the bacterial solution using biotechnology. Preferably, this step can remove multiple harmful bacteria simultaneously to further improve the safety of the bacterial solution.

[0101] K3. Continue to let the microbial mixture obtained in step K2 enter the beneficial bacteria magnetic bead tank 222. The second antibody magnetic bead 221 enters the beneficial bacteria magnetic bead tank 222 through the second material port 2242. The second stirring motor 2241 and its connected second stirring blade 2244 rotate and mix for 10 minutes. Then incubate at room temperature for 40 minutes to couple the beneficial bacteria antibody magnetic beads with the beneficial bacteria and obtain a beneficial bacteria couple body.

[0102] This application involves coupling beneficial bacteria magnetic beads that have undergone surface carboxyl group activation treatment with the corresponding antibody of the beneficial bacteria to obtain a second antibody magnetic bead 221. In actual operation, the second antibody magnetic bead 221 is prepared before being sent to the beneficial bacteria magnetic bead container 222 to avoid affecting the beneficial bacteria coupler obtained in this application. This application uses Bacteroides uniformis as an example to further explain how this application achieves the enrichment of beneficial bacteria, so as to make the technical solution of the present invention easier to understand.

[0103] K31, Carboxyl group activation on the surface of beneficial bacteria magnetic beads

[0104] In a clean bench, transfer 2000 mL of Mag COOH sterile magnetic bead suspension (70113-5, Suzhou Beaver Biotechnology) (magnetic bead concentration 10 mg / mL) to a 5000 mL sterile centrifuge bottle. Magnetic separation is performed to remove the supernatant. The mixture is then washed twice with 4000 mL of MEST solution (100 mL MEST, pH 5.0, 0.05% Tween 20), and the supernatant is removed. Immediately add 2000 mL of freshly prepared EDC solution (10 mg / mL, using the above MEST solution as a dispersant) and 2000 mL of NHS (10 mg / mL, using the above MEST solution as a dispersant) to the centrifuge tube containing the magnetic beads. Vortex to fully suspend the magnetic beads and activate at room temperature for 30 min. Transfer the magnetic bead suspension in 200 mL increments to new sterile centrifuge bottles. During this period, the magnetic beads are kept in suspension (this can be achieved by inverting the beads using a vertical mixer); after the above steps, the carboxyl groups on the surface of the magnetic beads are activated and can be covalently coupled with biological ligands containing primary amino groups. (The activated state should not be stored for a long time; immediate coupling is recommended.)

[0105] Covalent coupling of K32, magnetic beads, and beneficial bacteria antibodies

[0106] The monoclonal antibody buffer for *Bacteroides uniformis* was replaced with 15 mM MES buffer (pH 6.0), and the antibody was diluted to 5 mg / mL with MES buffer. 8 mL of the antibody was thoroughly mixed with 200 mL of activated carboxyl magnetic beads (10 μm in diameter). The mixture was incubated at room temperature for 2 h, keeping the magnetic beads in suspension during conjugation (this can be achieved by inverting the beads using a vertical mixer). Magnetic separation was performed, and the supernatant was aspirated while simultaneously detecting the remaining antibody content. The amount and concentration of antibody conjugated to the magnetic beads were calculated. The magnetic beads were washed 2 to 3 times and resuspended in physiological saline to obtain the monoclonal antibody magnetic beads for *Bacteroides uniformis*. These monoclonal antibody magnetic beads for *Bacteroides uniformis* can precisely enrich *Bacteroides uniformis*.

[0107] In practice, staff can replace *Bacteroides uniformis* with other harmful bacteria, such as *Faecalibacterium prausnitzii* and *Roseburia*. Simultaneously, during step K32, this application can place multiple beneficial bacteria antibody beads (i.e., second antibody beads 221) into the beneficial bacteria magnetic bead container 222 to simultaneously enrich multiple beneficial bacteria in the intestinal microbial mixture.

[0108] Furthermore, in order to improve work efficiency, the beneficial bacteria in this application may be one or more of the following: Bacteroides monomorpha, Faecalibacterium, Fecalcoccus, Ruminococcus, Bifidobacterium, Lactobacillus, Reuteria, Akkermania, Christensen, Vespasmid, Butymonas, Trichophyton, Chainspinipes, Vibrio butyricum, Streptococcus thermophilus, Bacillus, Butyricococcus, Eubacterium, Rosbyrate, and Osmidrosis.

[0109] K33 and the second antibody magnetic bead 221 bind to beneficial bacteria to obtain a beneficial bacteria coupler.

[0110] After processing in step K32, the mixture enters the beneficial bacteria magnetic bead tank 222. The aforementioned beneficial bacteria antibody magnetic beads (second antibody magnetic beads) are taken and mixed in a certain ratio (e.g., 1:1). Second antibody magnetic beads 221 are added to the beneficial bacteria magnetic bead tank 222 through the second material inlet 2242. The second antibody magnetic beads 221 and the intestinal microbial mixture are mixed. The second stirring motor 2241, with its attached second stirring blade 2244, rotates to mix for 10 minutes, followed by static incubation for 40 minutes. This allows the second antibody magnetic beads 221 to bind with the beneficial bacteria, resulting in a beneficial bacteria coupler.

[0111] In practice, the second antibody magnetic bead 221 can also be referred to as a harmful bacteria antibody magnetic bead. The methods for obtaining the second antibody magnetic bead 221 and the methods for coupling the second antibody magnetic bead 221 with harmful bacteria to form a harmful bacteria couple body are all conventional methods in the field and will not be described in detail here.

[0112] K4. The negative pressure pipeline carries the beneficial bacteria coupler into the beneficial bacteria adsorption bottle. Under the action of the second electromagnetic induction coil 2251, the beneficial bacteria coupler is positively enriched, thereby adsorbing the beneficial bacteria in the mixed liquid. At this time, the waste liquid can be collected by the waste liquid bottle.

[0113] This step aims to enrich any beneficial bacteria that may be present in the donor. Preferably, this step can enrich multiple beneficial bacteria simultaneously to further improve the collection efficiency of beneficial bacteria.

[0114] K5. Allow the beneficial bacteria coupler to enter the dissociation bottle, add the dissociation enzyme to the third material port 2342, mix well for 10 minutes, and incubate at room temperature for 30 minutes.

[0115] In this step, the beneficial bacteria coupler is introduced into a dissociation bottle, and the beneficial bacteria coupler is resuspended in physiological saline. REAlease magnetic bead dissociation reagent is added to the third material port 2342 to dissociate the second antibody magnetic bead 221 and the beneficial bacteria. Then, the mixture is incubated at room temperature for 30 minutes to separate the beneficial bacteria and the antibody magnetic beads.

[0116] In practice, the method of separating beneficial bacteria couplers into second antibody magnetic beads and beneficial bacteria using dissociation enzymes is a conventional approach in this field and will not be elaborated here.

[0117] K6. The negative pressure pipeline pushes the mixture in step K5 into the magnetic bead adsorption bottle 233. Under the action of the third electromagnetic induction coil 2351, the magnetic beads 221 with the second antibody are adsorbed to remove the second antibody magnetic beads 221 in the mixture in step K5, thereby obtaining pure beneficial bacteria.

[0118] K7. Beneficial bacteria enter the bacterial liquid collection bottle, thereby achieving the collection of beneficial bacteria.

[0119] In practice, the beneficial bacteria collected in step K7 can also be sequenced using 16S rDNA to identify the antigen-capturing bacteria.

[0120] To make the technical solution of this application easier to understand, this application also lists a beneficial bacteria catalog and a harmful bacteria catalog, as follows:

[0121] Table 1: List of Beneficial Bacteria

[0122]

[0123]

[0124]

[0125]

[0126] Table 2: List of Harmful Bacteria

[0127]

[0128]

[0129] All other parts not described in detail are existing technologies. Although the above embodiments provide a detailed description of the present invention, they are only some embodiments of the present invention, not all embodiments. People can obtain other embodiments based on this embodiment without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An automated microbial enrichment and adsorption device, characterized in that: It includes a negative pressure component (250), a beneficial bacteria enrichment component (220) for positive enrichment of beneficial bacteria in a microbial mixture, a beneficial bacteria dissociation component (230) for dissociation of beneficial bacteria couplers, and a beneficial bacteria collection bottle (240) for collecting beneficial bacteria. The negative pressure component (250) is used to allow the microbial mixture to flow sequentially through the beneficial bacteria enrichment component (220) and the beneficial bacteria dissociation component (230) before entering the beneficial bacteria collection bottle (240).

2. The automated microbial enrichment and adsorption device according to claim 1, characterized in that: The beneficial bacteria enrichment component (220) includes a second antibody magnetic bead (221), a beneficial bacteria magnetic bead container (222), and a beneficial bacteria enrichment bottle (223). The beneficial bacteria magnetic bead container (222) is provided with a beneficial bacteria coupling element (224) for coupling beneficial bacteria and the second antibody magnetic bead (221) together. The beneficial bacteria enrichment bottle (223) is provided with a beneficial bacteria enrichment element (225) for positive enrichment of the beneficial bacteria coupler.

3. The automated microbial enrichment and adsorption device according to claim 2, characterized in that: The beneficial bacteria enrichment component (220) also includes a waste liquid collection bottle (226). The beneficial bacteria enrichment bottle (223) is provided with a waste liquid inlet. The waste liquid collection bottle (226) is connected to the waste liquid inlet to collect the waste liquid after the forward enrichment treatment.

4. The automated microbial enrichment and adsorption device according to claim 2, characterized in that: The beneficial bacteria coupling component (224) includes a second stirring motor (2241) and a second material inlet (2242). The second material inlet (2242) is used to introduce second antibody magnetic beads (221) into the beneficial bacteria magnetic bead container (222). The output end of the second stirring motor (2241) is connected to a second stirring blade (2244) located in the beneficial bacteria magnetic bead container (222).

5. The automated microbial enrichment and adsorption device according to claim 2, characterized in that: The beneficial bacteria enrichment component (225) includes a second electromagnetic induction coil (2251) and a second coil controller (2252). The second electromagnetic induction coil (2251) is sleeved on the beneficial bacteria enrichment bottle (223). The second coil controller (2252) and the second electromagnetic induction coil (2251) are electrically connected to control the working state of the second electromagnetic induction coil (2251).

6. The automated microbial enrichment and adsorption device according to claim 1, characterized in that: The beneficial bacteria dissociation assembly (230) includes a beneficial bacteria dissociation tank (232) and a magnetic bead adsorption bottle (233). The beneficial bacteria dissociation tank (232) is provided with a beneficial bacteria dissociation element (234) for dissociating the beneficial bacteria coupler, and the magnetic bead adsorption bottle (233) is provided with a magnetic bead adsorption element (235) for adsorbing the second antibody magnetic beads (221).

7. The automated microbial enrichment and adsorption device according to claim 6, characterized in that: The beneficial bacteria dissociation device (234) includes a third stirring motor (2341) and a third material port (2342). The third material port (2342) is used to introduce dissociation enzymes into the beneficial bacteria dissociation tank (232). The output end of the third stirring motor (2341) is connected to a third stirring blade (2344) located in the beneficial bacteria dissociation tank (232).

8. The automated microbial enrichment and adsorption device according to claim 6, characterized in that: The magnetic bead adsorption component (235) includes a third electromagnetic induction coil (2351) and a third coil controller (2352). The third electromagnetic induction coil (2351) is sleeved on the magnetic bead adsorption bottle (233). The third coil controller (2352) and the third electromagnetic induction coil (2351) are electrically connected to control the working state of the third electromagnetic induction coil (2351).

9. The automated microbial enrichment and adsorption device according to any one of claims 1 to 8, characterized in that: It also includes a harmful bacteria adsorption component (210) for removing harmful bacteria from the microbial mixture. The negative pressure component (250) is used to allow the microbial mixture to flow sequentially through the harmful bacteria adsorption component (210), the beneficial bacteria enrichment component (220), and the beneficial bacteria dissociation component (230) before entering the beneficial bacteria collection bottle (240).

10. The automated microbial enrichment and adsorption device according to claim 9, characterized in that: The harmful bacteria adsorption assembly (210) includes a first antibody magnetic bead (211), a harmful bacteria magnetic bead container (212), and a harmful bacteria adsorption bottle (213). The harmful bacteria magnetic bead container (212) is provided with a harmful bacteria coupling element (214) for coupling the harmful bacteria and the first antibody magnetic bead (211) together. The harmful bacteria adsorption bottle (213) is provided with a harmful bacteria adsorption element (215) for reverse enrichment of the harmful bacteria coupler.

11. The automated microbial enrichment and adsorption device according to claim 10, characterized in that: The harmful bacteria coupling component (214) includes a first stirring motor (2141) and a first material inlet (2142). The first material inlet (2142) is used to introduce first antibody magnetic beads (211) into the harmful bacteria magnetic bead container (212). The output end of the first stirring motor (2141) is located in the first stirring blade (2144) inside the harmful bacteria magnetic bead container (212).

12. The automated microbial enrichment and adsorption device according to claim 10, characterized in that: The harmful bacteria adsorption component (215) includes a first electromagnetic induction coil (2151) and a first coil controller (2152). The first electromagnetic induction coil (2151) is sleeved on the harmful bacteria adsorption bottle (213). The first coil controller (2152) and the first electromagnetic induction coil (2151) are electrically connected to control the working state of the first electromagnetic induction coil (2151).

13. The automated microbial enrichment and adsorption device according to claim 9, characterized in that: It also includes an enrichment adsorption box (208), which is further provided with a first partition (261) and a second partition (262). The first partition (261) divides the interior of the enrichment adsorption box (208) into an operation area (263) and a background area (264). The second partition (262) divides the operation area (263) into a clean area (265) and a contaminated area (266). The harmful bacteria adsorption component (210), the beneficial bacteria enrichment component (220), and the beneficial bacteria dissociation component (230) are all located in the contaminated area (266). The beneficial bacteria collection bottle (240) is located in the clean area (265). The negative pressure component (250) is installed on the first partition (261).

14. The automated microbial enrichment and adsorption device according to claim 9, characterized in that: The negative pressure assembly (250) includes a first peristaltic pump (251) installed between the harmful bacteria adsorption assembly (210) and the beneficial bacteria enrichment assembly (220), a second peristaltic pump (252) installed between the beneficial bacteria enrichment assembly (220) and the beneficial bacteria dissociation assembly (230), and a third peristaltic pump (253) installed between the beneficial bacteria dissociation assembly (230) and the beneficial bacteria collection bottle (240).

15. The automated microbial enrichment and adsorption device according to claim 9, characterized in that: It also includes an odor purification bottle (271) and a negative pressure pump (272). One end of the negative pressure pump (272) is connected to the odor purification bottle (271) and the harmful bacteria adsorption component (210) in sequence, and the other end is connected to the buffer (273), the filter (274) and the beneficial bacteria collection bottle (240) in sequence.

16. The automated microbial enrichment and adsorption device according to claim 15, characterized in that: The buffer (273) includes a first buffer bottle (2731) and a second buffer bottle (2372) arranged sequentially along the gas flow direction.

17. The automated microbial enrichment and adsorption device according to claim 13, characterized in that: The clean area (265) is also equipped with a display controller (280), and the harmful bacteria adsorption component (210), the beneficial bacteria enrichment component (220) and the beneficial bacteria dissociation component (230) are all electrically connected to the display controller (280).

18. The automated microbial enrichment and adsorption device according to claim 13, characterized in that: The lower end of the enrichment adsorption box (208) is provided with a nitrogen cabinet (291) for storing nitrogen and a consumable cabinet (292) for storing consumables, with the nitrogen cabinet (291) located between the enrichment adsorption box (208) and the consumable cabinet (292).

19. The automated microbial enrichment and adsorption device according to claim 13, characterized in that: The enrichment adsorption box (208) is also equipped with one or more of the following: a printer (201), a fan (202), a buzzer (203), a spotlight (204), and a lighting lamp (205).

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