Plant disease seedling stage resistance identification combined device

Abstract

The utility model discloses a plant disease seedling-stage resistance identification combined device which comprises a fixing frame, a seedling-raising plate, an incubator, a heat preservation box and a spraying assembly. Seedling-raising holes of the seedling-raising plate are used for seed inoculation. The seedling raising plate is detachably connected to the fixed frame; a culture solution is contained in the culture box; the fixed frame is arranged in the culture box, and the seedling raising tray can be in contact with the liquid level of the culture solution to germinate seeds; the bottom of the incubator is open and covers the incubator, and the bottom end face of the incubator abuts against the top end face of the incubator; the spraying assembly is fixed in the heat preservation box or the culture box so as to spray spore suspension liquid to plant roots or plant leaves. According to the utility model, not only can seeds be cultivated to root and germinate, but also plant root systems and plant leaves can be inoculated in batches, the occurrence condition of diseases can be observed in real time, and the resistance identification work of indoor cotton seedling stage diseases can be assisted to be completed.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of agricultural biotechnology and plant pathology technology, more specifically relates to a plant disease seedling stage resistance identification combination device. BACKGROUND

[0002] Cotton, as one of the important economic crops in the world, has an irreplaceable position in the field of textile industry. However, the frequent occurrence of cotton Verticillium wilt seriously restricts the yield and quality improvement of cotton, and brings huge economic losses to the cotton industry. Accurate and efficient identification of cotton varieties against Verticillium wilt and Fusarium wilt is the key prerequisite for cotton disease resistance breeding and disease control.

[0003] Currently, in the field of cotton disease resistance identification, although the existing indoor identification methods and devices have made some progress, there are still many limitations. Traditional identification methods often rely on field natural disease observation, but this method is greatly affected by environmental factors (such as climate, soil conditions, etc.), has a long and unstable disease period, and is difficult to meet the needs of rapid and accurate identification. Although indoor inoculation identification technology partially overcomes some drawbacks of field identification, existing indoor devices still have significant shortcomings when applied to cotton Verticillium wilt identification. The Verticillium wilt pathogen mainly invades cotton plants through the roots or infects them from the leaves under certain conditions, but existing devices cannot conveniently and uniformly achieve root and leaf inoculation operations. When inoculating the roots, due to the lack of a precise inoculation system, it is difficult to ensure the uniform distribution of the pathogen around the root system, resulting in inconsistent inoculation concentrations and affecting the accuracy and repeatability of the identification results. In terms of leaf inoculation, there is a lack of corresponding functional design, which cannot simulate the natural infection pathway, and thus cannot comprehensively evaluate the resistance of cotton varieties to diseases.

[0004] In addition, in terms of environmental control, existing devices are difficult to maintain suitable temperature, humidity and other conditions during pathogen inoculation and disease occurrence, which is not conducive to stable disease occurrence and observation. At the same time, the monitoring of cotton root growth is not convenient and accurate, which cannot real-time grasp the dynamic changes of the roots after being infected by the pathogen, and is not conducive to the in-depth study of the relationship between disease occurrence mechanism and cotton resistance response.

[0005] Therefore, how to provide an efficient and reliable device that can meet the precise identification needs of cotton Verticillium wilt and Fusarium wilt, improve inoculation efficiency and quality, and provide strong technical support for cotton disease resistance breeding and disease control, is a problem that needs to be solved by those skilled in the art. UTILITY MODEL CONTENT

[0006] Therefore, the plant disease seedling resistance identification combined device can realize seed germination and rooting, and can realize batch resistance identification of soil-borne disease germplasm resources, and effectively improves inoculation efficiency and inoculation accuracy.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0008] A plant disease seedling resistance identification combined device, comprising:

[0009] A fixed frame,

[0010] A seedling raising tray, a plurality of seedling holes are arranged on the seedling raising tray for seed inoculation, and the bottom surface of the seedling raising tray is detachably connected to the top surface of the fixed frame.

[0011] A culture box, the culture box is open at the top and contains culture solution in the inner cavity, the fixed frame is arranged in the culture box, and the seedling raising tray can be in contact with the liquid level of the culture solution to make the seeds germinate.

[0012] An incubator, the bottom of the incubator is open and is buckled to the culture box, and the bottom end surface of the incubator is in abutment with the top end surface of the culture box.

[0013] A spraying assembly, the spraying assembly is fixed in the incubator or the culture box to spray the suspension on the leaves.

[0014] The seedling holes on the seedling raising tray can be filled with seeds, and the culture solution in the culture box can be in contact with the seeds to realize seed inoculation; the spraying system can be used for comprehensive and uniform inoculation of the leaves on the top surface of the seedling raising tray, and can realize separate seed root inoculation experiment and seedling stage leaf inoculation; through inoculation of the plant roots and the plant seedling leaves, the resistance of the plant to diseases can be identified, and the inoculation efficiency and inoculation quality of the crops can be greatly improved.

[0015] Preferably, the seedling raising tray comprises a panel and a seed cup, a plurality of seedling holes are arranged on the panel in an array, the number of the seed cups is plural, the openings and bottoms of the seed cups are through, the outer walls of the openings are fixed to the hole walls of the panel corresponding to the seedling holes, the bottoms are located in the inner cavity of the fixed frame and can be in contact with the culture solution, and the seeds are placed in the seed cups.

[0016] Preferably, a permeable membrane is fixed to the bottom of the plurality of seed cups, and a buoy is fixed to the bottom surface of the permeable membrane to float on the surface of the culture solution. The nutrient solution enters the seed cup through the permeable membrane and the opening in the bottom of the seed cup, providing the required nutrient medium for seed germination. The permeable membrane maintains the stability of the osmotic pressure, allowing the seed to uniformly absorb the nutrient solution, avoiding the problem of inaccurate water injection caused by the handheld water injector, and affecting the germination efficiency of the seed due to excessive or insufficient water injection, causing the seed to rot or dry out.

[0017] Preferably, the bottom surface of the panel is fixed with a magnet, and the top surface of the fixed frame is fixed with a magnetic sheet that magnetically interacts with the magnet. The quick and stable connection between the seedling tray and the fixed frame is achieved through magnetic adsorption. This connection method is convenient for disassembly and installation, and is convenient for cleaning, replacing the seed cup and the permeable membrane, and checking and maintaining the inside of the seedling tray. The efficient connection method can save device assembly and adjustment time, improve overall operation efficiency, and indirectly improve inoculation efficiency.

[0018] Preferably, the inner wall of the bottom of the seed cup is hinged with a louver to control the opening degree of the through hole in the bottom of the seed cup. By rotating the louver, the opening degree of the bottom can be controlled, so that the oxygen demand of the plant root system at different growth stages can be accurately adjusted. In the early stage of seed germination, the opening degree of the bottom can be appropriately adjusted to maintain a certain humidity; during the vigorous growth period of the root system, the opening degree of the bottom is increased to increase the supply of oxygen and promote the growth of the root system. This helps the plant root system to maintain a good growth state before inoculating the pathogenic bacteria, enhances the stress response ability to the pathogenic bacteria, and indirectly improves the inoculation effect.

[0019] Preferably, the fixed frame, the incubator and the incubator are made of acrylic plates, the inner wall of the incubator is fixed with LED lights and is electrically connected with the switch on the outer wall, and the opposite inner walls of the incubator are fixed with a plurality of cameras to shoot the plant root system. High-definition cameras are installed in the incubator, and the cameras are connected to computer image analysis software. The root system image is periodically shot, and the software automatically analyzes the length, diameter, branch number and other parameters of the root system using image recognition technology to generate a root growth curve and monitor the growth dynamics of the root system in real time, especially the changes after inoculating the pathogenic bacteria, providing data support for studying the influence of the disease on the growth of the root system. Timely and accurate root growth data can help evaluate the inoculation effect, optimize the inoculation strategy, and indirectly improve the inoculation quality.

[0020] Preferably, one side wall of the incubator is provided with scale marks perpendicular to and parallel to the length direction. The scale marks can accurately measure the length and growth direction of the root system. At the same time, in cooperation with the LED light in the incubator, sufficient light is provided when needed by switching control, so that the details of the root system are more clearly visible, and accurate observation of the root growth helps to judge the inoculation effect in time, optimize the inoculation scheme, and indirectly improve the inoculation quality.

[0021] Preferably, the spraying assembly comprises a first connecting pipe, a spraying pipe, a second connecting pipe, a liquid storage tank and a pressure pump; the spraying pipe is multiple and arranged side by side, and the outer wall is fixed to the inner wall of the top end of the incubator; the outer wall of the multiple spraying pipes is fixed with multiple atomizing nozzles; one end of the multiple spraying pipes is closed, and the other end is fixedly communicated with the outer wall of the first connecting pipe; one end of the second connecting pipe is fixedly communicated with the outer wall of the first connecting pipe away from the spraying pipe, and the other end is fixedly communicated with the liquid storage tank; and the pressure pump is fixed on the second connecting pipe. During inoculation, the spore suspension is injected into the liquid storage tank, and the pressure pump is started to uniformly spray the spore suspension around the root system, achieving efficient and uniform root inoculation; it can also be sprayed on the leaves to achieve leaf inoculation; the atomizing nozzles can ensure that the sprayed spore suspension is in mist form and uniformly distributed, and the pressure pump can adjust the spraying pressure to control the spraying speed and range of the spore suspension, ensuring that each plant can receive an equal amount of pathogenic spores, improving the consistency and accuracy of inoculation, and thus improving the inoculation quality.

[0022] Preferably, the incubator is provided with a liquid inlet hole at the top end of one side wall and a liquid outlet hole at the bottom end, and the liquid inlet hole and the liquid outlet hole can be plugged by sealing plugs. The spore suspension can be accurately injected into the incubator through a syringe or infusion tube and the like, so that the spore suspension can fully contact the root system; the liquid outlet hole facilitates replacement of the spore suspension or discharge of waste liquid when needed. The liquid outlet can be connected to a waste liquid collection container to ensure the cleanliness of the experimental environment. The convenient liquid inlet and outlet mode can improve the processing efficiency of the spore suspension and is conducive to the rapid switching of multiple inoculation tests, thereby improving the inoculation efficiency.

[0023] Preferably, the inner walls of the incubator and the incubator are fixed with multiple temperature or humidity sensors. Through the temperature or humidity sensors, the growth environment of the plants can be controlled to ensure that the plants maintain normal physiological state and improve the success rate and quality of inoculation.

[0024] Compared with the prior art, the plant disease seedling resistance identification combined device provided by the utility model has the advantages that the plant disease seedling resistance identification combined device can not only cultivate seeds and make them root and germinate, but also inoculate plant roots and plant leaves, and assist in completing plant disease resistance identification work, through the innovative design of the seedling tray, the inoculation system, the environment control and the observation and monitoring, a plant disease identification device integrating accurate inoculation, environment control and real-time monitoring is constructed, accurate and efficient identification of plant disease resistance is realized, and inoculation efficiency and inoculation quality are improved.

[0025] The utility model can be applied to cotton verticillium wilt resistance identification work, evaluate cotton variety disease resistance, and provide scientific basis for cotton breeding and disease control. ACCURACY

[0026] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are only the embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to the provided drawings without paying creative labor.

[0027] Fig. 1 The combined device structure schematic view provided by the utility model is shown in the figure.

[0028] Fig. 2 The combined device sectional view provided by the utility model is shown in the figure.

[0029] Fig. 3 The combined device explosion schematic view provided by the utility model is shown in the figure.

[0030] Fig. 4 The seedling tray provided by the utility model is shown in the figure.

[0031] Among them,

[0032] 1- incubator;

[0033] 2- spraying assembly;21- spraying pipe;22- first connecting pipe;23- second connecting pipe;24- pressure pump;25- liquid storage tank;

[0034] 3- seedling tray;31- panel;32- seed cup;33- louver;

[0035] 4- permeable membrane;

[0036] 5- fixed frame;

[0037] 6- incubator;61- liquid inlet hole;62- liquid outlet hole;63- scale mark;64- switch;

[0038] 7-camera;

[0039] 8-temperature or humidity sensor. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0041] Embodiment 1:

[0042] Referring to the drawings, Figs. 1-4 The embodiments of the present application disclose a plant disease seedling resistance identification combined device for performing a disease resistance identification test on plant root systems, comprising:

[0043] a fixed frame 5,

[0044] a seedling tray 3, a plurality of seedling holes are arranged on the seedling tray 3 to perform seed germination and inoculation; the bottom surface of the seedling tray 3 is detachably connected to the top surface of the fixed frame 5;

[0045] a culture box 6, the culture box 6 is open at the top and contains a culture solution in the inner cavity; the fixed frame 5 is arranged in the culture box 6 and the seedling tray 3 can be in contact with the liquid level of the culture solution to make the seeds germinate;

[0046] an incubator 1, the incubator 1 is open at the bottom and buckled to the culture box 6, and the bottom end surface of the incubator 1 abuts against the top end surface of the culture box 6;

[0047] a spraying assembly 2, the spraying assembly 2 is fixed in the culture box 6 to spray the suspension to the plant root systems.

[0048] In the embodiment, the seedling tray 3 comprises a panel 31 and seed cups 32; a plurality of seedling holes are arranged in an array on the panel 31; the plurality of seed cups 32 are in through communication between the cup mouths and the cup bottoms, and the outer walls of the cup mouths are fixed one by one in correspondence with the hole walls of the seedling holes on the panel 31, and the cup bottoms are located in the inner cavity of the fixed frame 5 and can be in contact with the culture solution.

[0049] In order to further optimize the above technical solution, a permeable membrane 4 is further arranged, the permeable membrane 4 is bonded and fixed to the cup bottoms of the plurality of seed cups 32, and a float is fixed to the bottom surface of the permeable membrane 4 to float on the liquid level of the culture solution.

[0050] In the seed germination cultivation stage, the culture solution can be distilled water, uniform and full seeds are selected, and the single seeds are respectively placed into the seed cups through the seedling holes, the combined seedling tray is placed into the incubator, water enters the seed cups through the constant osmotic membrane and the air vents from the bottom of the seedling tray to provide the required water for the seed germination, the water received by the seeds is uniform, and the seeds will not sink or separate from the water, thereby improving the germination survival rate of the cotton seeds; after the plant germination and rooting, the water can be replaced with a spore suspension for inoculation of the plant root system, so that the disease symptoms of the plant root system are recorded.

[0051] In order to further optimize the above technical solution, the side wall of the incubator 6 is provided with an inlet hole 61 at the top end and an outlet hole 62 at the bottom end, and the inlet hole 61 and the outlet hole 62 are both blocked by a sealing plug.

[0052] The spore suspension can be accurately injected into the incubator through a syringe or an infusion tube. The position of the inlet hole is close to the bottom, so that the spore suspension can fully contact the root system. A drainage hole is also provided at the bottom of the incubator to facilitate the replacement of the spore suspension or the discharge of waste liquid when needed. The drainage hole can be connected to a waste liquid collection container to ensure the cleanliness of the experimental environment. The convenient liquid inlet and outlet mode can improve the processing efficiency of the spore suspension and facilitate the rapid switching of multiple inoculation tests, thereby improving the inoculation efficiency.

[0053] In order to further optimize the above technical solution, the spraying assembly 2 includes a first connecting pipe 22, a spraying pipe 21 located in the incubator 6, a second connecting pipe 23 located outside the incubator 6, a liquid storage tank 25 and a pressure pump 24; the spraying pipe 21 is arranged in parallel and the outer wall is fixed to the inner wall at the top end of the incubator 1, a plurality of atomizing nozzles are fixed to the outer wall of the plurality of spraying pipes 21; one end of the plurality of spraying pipes 21 is closed, the other end is fixedly communicated with the outer wall of the first connecting pipe 22, the outer wall of the incubator 6 is provided with a pipe hole, one end of the second connecting pipe 23 penetrates through the pipe hole and is fixedly communicated with the outer wall of the first connecting pipe 22 away from the spraying pipe 21, and the other end is fixedly communicated with the liquid storage tank 25; the pressure pump 24 is fixed on the second connecting pipe 23.

[0054] When the root system is inoculated, the spore suspension can be injected into the liquid storage tank, the aperture of the atomizing nozzle is between 0.1-0.5mm, ensuring that the sprayed spore suspension is in mist form and uniformly distributed. Start the pressure pump, adjust the pressure and spraying speed, so that the spore suspension is uniformly sprayed around the root system. After the inoculation is completed, the pressure pump is turned off, the disease condition of the root system is observed, the growth state and disease symptoms of the root system are recorded regularly, and the changes of the root system growth parameters are monitored by using a camera and image analysis software.

[0055] In order to further optimize the above technical scheme, improve the efficiency of the device, the bottom surface of the panel 31 is fixed with a magnet, and the top surface of the fixed frame 5 is fixed with a magnetic sheet magnetically connected with the magnet. The seedling tray and the fixed frame are connected quickly and stably through magnetic adsorption. This connection method is convenient for disassembly and installation, and is convenient for cleaning, replacing the seed cup and the permeable membrane, and checking and maintaining the inside of the seedling tray. The efficient connection method can save the device assembly and adjustment time, improve the overall operation efficiency, and indirectly improve the inoculation efficiency.

[0056] In order to further optimize the above technical scheme, the inner wall of the cup bottom of the seed cup 32 is hinged with a louver 33 to control the opening degree of the through hole of the cup bottom of the seed cup 32. The opening degree of the cup bottom of the seed cup is controlled by the louver, so that the oxygen demand of the root system at different growth stages can be accurately adjusted.

[0057] In order to further optimize the above technical scheme, the fixed frame 5, the incubator 6 and the incubator 1 are all made of acrylic plate. The inner wall of the incubator 6 is fixed with an LED lamp and is electrically connected with the switch 64 of the outer wall. The opposite inner walls of the incubator 6 are fixed with a plurality of cameras 7 to shoot the plant roots.

[0058] High-definition cameras are installed in the incubator, and the cameras are connected to computer image analysis software. The root system image is periodically shot, and the software automatically analyzes the length, diameter, branch number and other parameters of the root system by using image recognition technology, generates a root growth curve, and monitors the root growth dynamic in real time, especially the change after inoculation of pathogenic bacteria, to provide data support for the study of the influence of disease on root growth. Timely and accurate root growth data can help evaluate the inoculation effect, optimize the inoculation strategy, and indirectly improve the inoculation quality.

[0059] In order to further optimize the above technical scheme, the side wall of the incubator 6 is provided with scale marks perpendicular to and parallel to the length direction.

[0060] The scale marks are arranged on the bottom and side of the incubator, which can accurately measure the length and growth direction of the roots. At the same time, the LED lamp in the incubator is controlled by a switch to provide sufficient light when needed, so that the details of the roots are more clearly visible. Accurate observation of the growth of the roots helps to judge the inoculation effect in time, optimize the inoculation scheme, and indirectly improve the inoculation quality.

[0061] In order to further optimize the above technical scheme, a plurality of temperature or humidity sensors 8 are fixed on the inner wall of the incubator 6. A micro humidifier is fixed on the inner wall of the incubator, and a ventilation device is fixed on the outer wall.

[0062] A heating and refrigeration element, such as a small ceramic heating sheet and a semiconductor refrigeration sheet, is installed around the incubator, connected to an intelligent temperature controller, and the temperature controller is communicatively connected to a temperature sensor. The temperature controller can automatically adjust the heating or refrigeration power according to the preset temperature range (e.g. 20-30℃), ensuring that the internal temperature of the device is always within the range suitable for the growth of pathogenic bacteria and the occurrence of plant diseases, and the temperature control accuracy can reach ±1℃. A stable temperature environment is conducive to the activity and infection process of pathogenic bacteria, and also helps to maintain the normal physiological state of the plant, improving the success rate and quality of inoculation.

[0063] The humidity sensor in the incubator monitors the humidity changes in real time. When the humidity is lower than the set value (e.g. 60%-80%), a micro humidifier is used to automatically increase the water vapor to increase the humidity; when the humidity is higher than the set value, a ventilation device is started to speed up air circulation to reduce humidity, so that the humidity is maintained within a stable range, which is conducive to the occurrence and development of diseases. Suitable humidity conditions can promote the germination and infection of pathogenic spores, while avoiding the growth of miscellaneous bacteria or poor plant growth due to excessive humidity, thereby improving the efficiency and quality of inoculation.

[0064] Example 2:

[0065] The utility model discloses a plant disease seedling stage resistance identification combined device, and through the spraying assembly, the disease resistance identification test of plant leaf is carried out, and the spraying assembly 2 includes the first connecting pipe 22 in the incubator 1, the spraying pipe 21 and the second connecting pipe 23 outside the incubator 1, the liquid storage tank 25 and the pressure pump 24, the spraying pipe 21 is multiple and is arranged in parallel, and the outer wall is fixed in the inner wall of the top end of the incubator 1, a plurality of atomizing nozzles are fixed to the outer wall of the multiple spraying pipes 21, one end of the multiple spraying pipes 21 is closed, the other end is fixedly communicated with the outer wall of the first connecting pipe 22, the outer wall of the incubator 1 is provided with a pipeline hole, one end of the second connecting pipe 23 penetrates the pipeline hole and is fixedly communicated with the outer wall of the first connecting pipe 22 away from the spraying pipe 21, and the other end is fixedly communicated with the liquid storage tank 25, and the pressure pump 24 is fixed on the second connecting pipe 23.

[0066] In other specific embodiments, the spraying assembly can also include a container and a dropper, the container is detachably connected in the incubator, the detachable connection of the container facilitates cleaning and replacement, the container contains spore suspension, one end of the drip irrigation is fixedly communicated with the bottom of the container, and the other end is fixedly provided with an atomizing nozzle, by adjusting the height or pressure of the container, the speed of spore suspension dripping from the dropper and the atomizing degree can be controlled. When carrying out leaf inoculation, the incubator is buckled to the seedling tray, the nozzle is aligned with the leaf, and the spore suspension is slowly released to realize uniform inoculation of the leaf. The leaf inoculation mode can simulate the natural infection path, so that the leaf inoculation is more uniform and controllable, and the inoculation efficiency and quality are improved.

[0067] To further optimize the above technical solutions, a plurality of temperature or humidity sensors 8 are fixed on the inner wall of the incubator 1.

[0068] A heating and refrigeration element, such as a small ceramic heating sheet and a semiconductor refrigeration sheet, is installed around the incubator, connected to an intelligent temperature controller, which is in communication with the temperature sensor. The temperature controller can automatically adjust the heating or refrigeration power according to the preset temperature range (e.g. 20-30℃), ensuring that the temperature inside the incubator is always within the range suitable for the growth of pathogenic bacteria and the occurrence of plant diseases, with a temperature control accuracy of ±1℃. A stable temperature environment is conducive to the activity and infection process of pathogenic bacteria, and also helps to maintain the normal physiological state of the plant, improving the success rate and quality of inoculation.

[0069] To further optimize the above technical solutions, a plurality of angle-adjustable small cameras are provided around the device to capture the leaves, stems and other parts of the plant from all directions. When the plant becomes diseased, the camera records the time of onset, the development process and the severity of the disease. At the same time, combined with manual observation and recording, the morphology, color, distribution and other characteristics of the disease spots are described in detail, providing a comprehensive basis for accurately judging the disease resistance of the plant. Comprehensive disease records help to accurately assess the inoculation effect and the disease resistance of the plant, providing a reference for subsequent improvement of the inoculation method and improving the quality of inoculation.

[0070] Example 3:

[0071] In combination with Example 1 and Example 2, during the cotton disease resistance identification experiment, the bottom incubator is assembled with the seedling tray according to the design requirements to ensure tight connection and no leakage. The container and spray pipe inside the incubator are installed, and the pipelines and sealing devices connected to the liquid inlet and outlet are connected. The incubator is fastened to the top of the incubator, and the temperature adjustment device is adjusted to ensure accurate temperature control. The sprayable assembly inside the incubator is installed, and the connection is checked to ensure that the atomization effect meets the requirements. The heating and refrigeration elements, humidity sensors and cameras are installed, connected to the corresponding controllers and computer software, and the system is debugged to ensure that the environmental control and data monitoring functions are running normally.

[0072] Healthy and plump cotton seeds are selected and subjected to disinfection treatment. The disinfected seeds are sown in seed cups, and the incubator is filled with water. The device is placed in a suitable environment for cultivation, and the cotton seedlings are grown to the appropriate stage. The seedling tray is removed and inoculated with pathogenic bacteria.

[0073] Root inoculation operation

[0074] According to the identification requirements, an appropriate amount of Verticillium wilt or Fusarium wilt spore suspension is prepared, and the spore suspension is injected into the liquid storage tank. The pressure pump of the spray system is started, and the pressure and spray speed are adjusted to ensure that the spore suspension is uniformly sprayed around the cotton roots.

[0075] In other specific embodiments, the liquid storage tank can also be removed, and the pressure pump is installed in the incubator, which directly replaces the liquid storage tank. The spore suspension is injected into the incubator through the liquid inlet, and attention should be paid to control the injection amount to avoid too much or too little liquid.

[0076] After inoculation is completed, the pressure pump is turned off, the root system is observed for disease occurrence, and the growth state and disease symptoms of the root system are recorded regularly. At the same time, the camera and image analysis software are used to monitor the changes of root growth parameters.

[0077] Leaf inoculation operation

[0078] The prepared spore suspension is loaded into the container in the incubator, and the height or pressure of the container is adjusted so that the spore suspension can slowly drip from the atomizing nozzle at the end of the dropper. The incubator is buckled on the seedling tray to ensure that the nozzle is aimed at the cotton leaves. The amount of spore suspension dripping and the degree of atomization are controlled as needed to achieve uniform inoculation of the leaves. After inoculation, the seedling tray is placed under the incubator cover to maintain appropriate temperature and humidity, and the leaf disease occurrence is observed. The disease occurrence time, symptom performance and other information are recorded, and the camera is used to record the disease process.

[0079] Environmental maintenance and data recording

[0080] During the entire identification process, the appropriate temperature and humidity environment is maintained through the temperature controller and humidity adjusting device. The spore suspension level and concentration in the incubator are checked regularly, and replenishment or replacement is performed in a timely manner if necessary. The experimental environment is kept clean and hygienic to prevent bacterial contamination from affecting the identification results. The cotton growth data (such as plant height, stem diameter, leaf number, root morphology, etc.), inoculation data (pathogen species, inoculation amount, inoculation time, inoculation site, etc.), environmental data (temperature and humidity change curve), and disease data (disease occurrence time, symptom type, severity, etc.) are recorded in detail to provide accurate data support for subsequent analysis and research. The images and curves generated by the data monitoring and recording system are used for in-depth analysis to evaluate the disease resistance of cotton varieties and provide scientific basis for cotton breeding and disease control. At the same time, the inoculation scheme is continuously optimized according to the inoculation effect and data feedback to further improve the inoculation efficiency and quality.

[0081] The embodiments in the specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple, and the related parts can be referred to the method part.

[0082] The above description of disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A plant disease seedling resistance identification combination device, characterized in that, The utility model relates to a seedling raising device, which comprises: a fixed frame (5), a seedling tray (3) with a plurality of seedling holes arranged at intervals for seed inoculation, the bottom surface of the seedling tray (3) being detachably connected to the top surface of the fixed frame (5); a culture box (6) with an open top and containing culture solution, the fixed frame (5) being placed in the culture box (6) and the seedling tray (3) being in contact with the liquid level of the culture solution to make the seeds germinate; a heat preservation box (1) with an open bottom and buckling the culture box (6), the bottom end surface of the heat preservation box (1) being in abutment with the top end surface of the culture box (6); a spraying assembly (2) fixed in the heat preservation box (1) or the culture box (6) to spray the plant roots or leaves with a suspension.

2. A plant disease seedling resistance identification combination device according to claim 1, characterized in that, The seedling tray (3) comprises a panel (31) and seed cups (32), the panel (31) being provided with a plurality of seedling holes arranged in an array, and the plurality of seed cups (32) having through openings and bottoms, the outer walls of the openings being fixed to the panel (31) corresponding to the hole walls of the seedling holes, and the bottoms being located in the inner cavity of the fixed frame (5) and being in contact with the culture solution.

3. A plant disease seedling resistance identification combination device according to claim 2, characterized in that, The utility model further comprises a permeable membrane (4) fixed to the bottoms of the plurality of seed cups (32), the bottom surface of the permeable membrane (4) being fixed with a float to float on the liquid level of the culture solution.

4. A plant disease seedling resistance identification combination device according to claim 3, characterized in that, The bottom surface of the panel (31) is fixed with magnets at four corners, and the top surface of the fixed frame (5) is fixed with magnetic sheets magnetically connected to the magnets.

5. A plant disease seedling resistance identification combination device according to claim 4, characterized in that, The inner wall of the bottom of the seed cup (32) is hinged with a louver (33) to control the opening degree of the through hole of the bottom of the seed cup (32).

6. The plant disease seedling resistance identification combination device according to claim 1, characterized in that, The fixed frame (5), the culture box (6) and the heat preservation box (1) are all made of acrylic plates, the inner wall of the culture box (6) is fixed with LED lamps, the outer wall of the culture box (6) is electrically connected with a switch (64), and the inner walls of the opposite sides of the culture box (6) are fixed with a plurality of cameras (7) to shoot the plant roots.

7. A plant disease seedling resistance identification combination device according to claim 6, characterized in that, One side wall of the culture box (6) is provided with scale marks (63) perpendicular to and parallel to the length direction.

8. The plant disease seedling resistance identification combination device according to claim 1, characterized in that, The spraying assembly (2) comprises a first connecting pipe (22), a plurality of spraying pipes (21), a second connecting pipe (23), a liquid storage tank (25) and a pressure pump (24), the plurality of spraying pipes (21) being fixed to the inner wall of the top end of the heat preservation box (1) and being provided with a plurality of atomizing nozzles, one end of the plurality of spraying pipes (21) being closed, the other end being fixedly connected to the outer wall of the first connecting pipe (22), one end of the second connecting pipe (23) being fixedly connected to the outer wall of the first connecting pipe (22) away from the spraying pipes (21), the other end being fixedly connected to the liquid storage tank (25), and the pressure pump (24) being fixed to the second connecting pipe (23).

9. The plant disease seedling resistance identification combination device according to claim 1, characterized in that, A liquid inlet hole (61) is formed in the top end of the side wall of the incubator (6), and a liquid outlet hole (62) is formed in the bottom end, and the liquid inlet hole (61) and the liquid outlet hole (62) can be sealed by sealing plugs.

10. The plant disease seedling resistance identification combination device according to claim 1, characterized in that, A plurality of temperature or humidity sensors (8) are fixed to the inner walls of the incubator (6) and the incubator (1).

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