Immunomodulatory cell scraping assembly

Abstract

The utility model relates to the technical field of biological cells, in particular to an immunomodulatory cell scraping component which is used for adjusting the flatness of equipment through an adjusting device, preventing culture solution in a culture dish from being non-uniformly distributed to influence the scraping operation of normal immune cells, adapting to culture dishes with different sizes through a fixing device, and improving the scraping efficiency. A culture dish is fixed in an auxiliary manner, and cells in the culture dish are scraped through a scraping device, so that the practicability of the device is improved; comprising an adjusting device, a fixing device and a scraping device, the fixing device is installed on the adjusting device, and the scraping device is installed on the adjusting device.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of biological cells, in particular to an immune regulation cell scraping assembly. BACKGROUND

[0002] Cell scraping is a common operation in biological research and medical diagnosis, involving the collection of cells from biological bodies or tissue samples; in the research fields of cell biology, molecular biology, etc., cell scraping is used to obtain cell samples for subsequent cell culture, gene analysis, protein detection, etc.; during immune cell culture, when the cells reach a certain density or need to be harvested, cell scraping can be used to scrape the cells from the culture dishes or culture bottles. During operation, the cell scraper should be clean, sterile, and avoid causing excessive damage to the cells.

[0003] The prior art Chinese utility model patent with application number CN202221268554.6 relates to a cell scraping device for cell culture tools, which includes a scraper mechanism, an electric mechanism, a support mechanism, a magnetic bottom plate, a stepper motor, a control switch, a sliding rail, a cantilever, and a counterweight base, etc., which can greatly improve the work efficiency of scraping cells on cell culture tools, save manpower and resources, and improve work efficiency.

[0004] However, the above-mentioned device does not have the ability to adapt to different thicknesses of culture dishes independently in actual use, and the scraping height of the device needs to be adjusted by the operator according to the thickness of the placed culture dish, increasing the labor intensity of the operator. UTILITY MODEL CONTENT

[0005] To solve the above technical problems, the utility model provides an immune regulation cell scraping assembly which adjusts the flatness of the device itself through an adjusting device, prevents uneven distribution of culture medium in the culture dish, affects normal immune cell scraping operations, adapts to different sizes of culture dishes through a fixing device, assists in fixing the culture dish, and scrapes cells in the culture dish through a scraping device, improving the practicality of the device.

[0006] The utility model relates to an immune regulation cell scraping assembly; it includes an adjusting device, a fixing device, and a scraping device, the fixing device is installed on the adjusting device, and the scraping device is installed on the adjusting device; the flatness of the device itself is adjusted through the adjusting device, uneven distribution of culture medium in the culture dish is prevented, normal immune cell scraping operations are affected, different sizes of culture dishes are adapted to through the fixing device, the culture dish is assisted in being fixed, and cells in the culture dish are scraped through the scraping device, improving the practicality of the device.

[0007] Preferably, the adjusting device comprises a base plate, adjusting nuts, leveling bolts, bearings and feet, a group of adjusting nuts are arranged at the four corners of the upper end surface of the base plate, leveling bolts are connected to the adjusting nuts, the lower part of the leveling bolts is connected to the upper end surface of the feet through bearings; the height position of the leveling bolts on the adjusting nuts is adjusted by rotating the leveling bolts, so that the levelness of the whole base plate is adjusted, a horizontal and stable operation environment is provided for the culture dish, and the practicability of the device is improved.

[0008] Preferably, the fixing device comprises an annular limiting seat, sliding plates, handle grooves, tension springs and arc-shaped fixing plates, the right part of the upper end surface of the base plate is provided with an annular limiting seat, the left and right parts of the upper end surface of the annular limiting seat are respectively provided with a group of rectangular sliding grooves, the two groups of sliding grooves extend to the outside of the annular limiting seat, a group of sliding plates are respectively placed in the two groups of sliding grooves, handle grooves are arranged on the outer part of the upper end surface of the sliding plates, the handle grooves and the outer side surface of the annular limiting seat are connected through tension springs, and arc-shaped fixing plates are installed on the inner part of the upper end surface of the sliding plates; the sliding plates are pulled outwards through the handle grooves, then the culture dish is placed in the annular limiting seat, and then the handle grooves are loosened, the sliding plates are powered by the tension springs to cooperate with the arc-shaped fixing plates to fix the culture dish, the influence of shaking or displacement of the culture dish itself on the cell scraping process during operation is prevented, different sizes of culture dishes can be fixed, and the practicability of the device is improved.

[0009] Preferably, it further comprises drainage holes, arc-shaped water retaining edges and drainage grooves, a group of arc-shaped water retaining edges are arranged on the left and right sides of the annular limiting seat, the two groups of arc-shaped water retaining edges are located outside the sliding grooves, the inner lower end surface of the arc-shaped water retaining edge is provided with a drainage hole, and the rectangular sliding groove is provided with a drainage groove; the liquid in the annular limiting seat is discharged through the drainage groove arranged in the rectangular sliding groove in the annular limiting seat, the liquid in the annular limiting seat is discharged when the culture dish is poured or the inside of the annular limiting seat is cleaned, the cleaning work of the operator is facilitated, the liquid flowing out of the drainage groove is guided and discharged through the arc-shaped water retaining edge and the drainage hole, the pollution of the liquid to the upper end surface of the base plate is prevented, and the practicability of the device is improved.

[0010] Preferably, the scraping device comprises a horizontal electric cylinder, a vertical electric cylinder, a rotating shaft base, a fastening bolt, a scraper frame and a scraper, the upper end face of the left part of the bottom plate is provided with the horizontal electric cylinder through a support, the moving end of the horizontal electric cylinder is connected with the vertical electric cylinder, the moving end of the vertical electric cylinder is provided with the rotating shaft base, the outer side face of the rotating shaft base is provided with the fastening bolt, the end of the fastening bolt extends to the inside of the rotating shaft base and can be in contact with the rotating shaft side face of the rotating shaft base, the rotating shaft side face of the rotating shaft base is connected with the scraper frame, the lower part of the scraper frame is provided with a mounting groove, and the scraper is fixed in the mounting groove through bolts; the cell scraping operation is completed through the cooperation of the scraper and the extension length of the horizontal electric cylinder and the vertical electric cylinder, a plurality of groups of bolts in the mounting groove of the lower part of the scraper frame adapt to scrapers of different lengths, the operation angle of the scraper is adjusted through the rotating shaft base to meet different scraping requirements, the operation angle of the scraper is fixed by rotating the fastening bolt to press the rotating shaft part of the rotating shaft base, and the practicability of the device is improved.

[0011] Preferably, the device further comprises a limiting electric cylinder, a pressure switch and a rubber contact, the right end face of the vertical electric cylinder is provided with the limiting electric cylinder, and the moving end of the limiting electric cylinder is connected with the rubber contact through the pressure switch; the extension length of the moving end of the limiting electric cylinder is controlled so that the lower end face of the rubber contact is lowered to a required position below the bottom of the scraper, when the vertical electric cylinder is elongated to the point that the rubber contact is pressed to the culture dish, the pressure switch senses that the pressure is greater than a preset value, thereby controlling the vertical electric cylinder to stop elongation and controlling the limiting electric cylinder to contract, and then the device can perform the scraping operation, the automation degree of the device and the adaptability to culture dishes of different thicknesses are improved, and the practicability of the device is improved.

[0012] Compared with the prior art, the device has the beneficial effects that: the flatness of the device itself is adjusted through the adjusting device, the uneven distribution of the culture solution in the culture dish is prevented, the normal scraping operation of the immune cells is affected, different sizes of culture dishes are adapted through the fixing device, the culture dishes are fixed, the cells in the culture dishes are scraped through the scraping device, and the practicability of the device is improved. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 is a first axonometric structure schematic view of the utility model;

[0014] Figure 2 is a second axonometric structure schematic view of the utility model;

[0015] Figure 3 is a first cross-sectional structure schematic view of the utility model;

[0016] Figure 4 is a second cross-sectional structure schematic view of the utility model;

[0017] Figure 5 is a local enlarged structure schematic view of the utility model;

[0018] Marked in the drawing: 1, bottom plate; 2, adjusting nut; 3, leveling bolt; 4, bearing; 5, foot; 6, annular limiting seat; 7, sliding plate; 8, handle slot; 9, tension spring; 10, arc-shaped fixed plate; 11, drainage hole; 12, arc-shaped water retaining edge; 13, drainage groove; 14, horizontal electric cylinder; 15, vertical electric cylinder; 16, rotating shaft seat; 17, fastening bolt; 18, scraper holder; 19, scraper; 20, limiting electric cylinder; 21, pressure switch; 22, rubber contact. DETAILED DESCRIPTION

[0019] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. The present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0020] EMBODIMENT

[0021] As Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 The fixing device is installed on the adjusting device, and the scraping device is installed on the adjusting device.

[0022] First, the leveling bolt 3 is rotated to adjust the height position of the leveling bolt 3 on the adjusting nut 2, thereby adjusting the levelness of the whole bottom plate 1 itself, then the sliding plate 7 is pulled outwards through the handle slot 8, then the culture dish is placed in the annular limiting seat 6, then the handle slot 8 is loosened, the sliding plate 7 is powered by the tension spring 9, and the culture dish is fixed by cooperating with the arc-shaped fixed plate 10, then the extension length of the moving end of the limiting electric cylinder 20 is controlled, so that the lower end surface of the rubber contact 22 is lowered to the required position below the bottom of the scraper 19, then the vertical electric cylinder 15 is controlled to extend until the rubber contact 22 is pressed to the culture dish, the pressure switch 21 senses that the pressure is greater than the preset value, thereby controlling the vertical electric cylinder 15 to stop extending and controlling the limiting electric cylinder 20 to contract, then the equipment can perform scraping operation.

[0023] The adjusting device comprises a bottom plate 1, an adjusting nut 2, a leveling bolt 3, a bearing 4 and a foot 5, four groups of adjusting nuts 2 are arranged at the upper end surfaces of the four corner edges of the bottom plate 1 respectively, the leveling bolt 3 is connected to the adjusting nut 2, and the lower part of the leveling bolt 3 is connected to the upper end surface of the foot 5 through the bearing 4.

[0024] The fixing device comprises a ring-shaped limiting seat 6, a sliding plate 7, a handle slot 8, a tension spring 9 and an arc-shaped fixing plate 10, the right part of the upper end surface of the bottom plate 1 is provided with the ring-shaped limiting seat 6, the left part and the right part of the upper end surface of the ring-shaped limiting seat 6 are respectively provided with a group of rectangular sliding grooves, both groups of sliding grooves extend to the outside of the ring-shaped limiting seat 6, a group of sliding plates 7 are respectively placed in both groups of sliding grooves, the outer part of the upper end surface of the sliding plate 7 is provided with the handle slot 8, the handle slot 8 is connected with the outer side surface of the ring-shaped limiting seat 6 through the tension spring 9, and the inner part of the upper end surface of the sliding plate 7 is provided with the arc-shaped fixing plate 10;

[0025] The device further comprises a drainage hole 11, an arc-shaped water-blocking edge 12 and a drainage groove 13, the left side and the right side of the ring-shaped limiting seat 6 are respectively provided with a group of arc-shaped water-blocking edges 12, both groups of arc-shaped water-blocking edges 12 are located outside the sliding groove, the inner lower end surface of the arc-shaped water-blocking edge 12 is provided with the drainage hole 11, and the rectangular sliding groove is provided with the drainage groove 13;

[0026] The scraping device comprises a horizontal electric cylinder 14, a vertical electric cylinder 15, a rotating shaft seat 16, a fastening bolt 17, a scraper frame 18 and a scraper 19, the left part of the upper end surface of the bottom plate 1 is provided with the horizontal electric cylinder 14 through a support, the moving end of the horizontal electric cylinder 14 is connected with the vertical electric cylinder 15, the moving end of the vertical electric cylinder 15 is provided with the rotating shaft seat 16, the outer side surface of the rotating shaft seat 16 is provided with the fastening bolt 17, the tail end of the fastening bolt 17 extends to the inside of the rotating shaft seat 16 and can be in contact with the rotating shaft side surface of the rotating shaft seat 16, the rotating shaft side surface of the rotating shaft seat 16 is connected with the scraper frame 18, the lower part of the scraper frame 18 is provided with a mounting groove, and the scraper 19 is fixed in the mounting groove through bolts;

[0027] The device further comprises a limiting electric cylinder 20, a pressure switch 21 and a rubber contact 22, the right end surface of the vertical electric cylinder 15 is provided with the limiting electric cylinder 20, and the moving end of the limiting electric cylinder 20 is connected with the rubber contact 22 through the pressure switch 21;

[0028] The adjusting device is used for adjusting the flatness of the device itself, preventing the uneven distribution of the culture solution in the culture dish and affecting the scraping operation of normal immune cells, the fixing device is used for fixing the culture dish of different sizes, the scraping device is used for scraping the cells in the culture dish, and the practicability of the device is improved.

[0029] As Figures 1 to 5As shown, the immune regulation cell scraping assembly of the utility model, in working, first rotate leveling bolt 3 and adjust the height position of leveling bolt 3 on adjusting nut 2, thereby the levelness of bottom plate 1 whole body is adjusted, then slide plate 7 is dragged outward by handle slot 8, then place culture dish in annular limit seat 6, then loosen handle slot 8, and slide plate 7 is powered by tension spring 9 and cooperates with arc-shaped fixed plate 10 to fix culture dish, then control the telescopic length of limit electric cylinder 20 moving end, make the lower end surface of rubber contact 22 lower than the bottom of scraper 19 to the required position, then control vertical electric cylinder 15 to elongate to when rubber contact 22 is extruded to culture dish, pressure switch 21 senses that pressure is greater than preset value to control vertical electric cylinder 15 to stop elongation and control limit electric cylinder 20 to contract, then equipment carries out scraping operation.

[0030] The horizontal electric cylinder 14, the vertical electric cylinder 15, the limit electric cylinder 20 and the pressure switch 21 of the immune regulation cell scraping assembly of the utility model are purchased on the market, and the technical personnel in the industry only need to install and operate according to the attached instruction manual, without the creative labor of the technical personnel in the field.

[0031] The above is only the preferred embodiment of the utility model, and it should be pointed out that for ordinary technical personnel in the technical field, without departing from the technical principle of the utility model, a number of improvements and modifications can be made, and these improvements and modifications should be regarded as the protection range of the utility model.

Claims

1. An immunomodulatory cell scraping assembly; characterized in that, The utility model relates to a kind of adjustable scraping device, including adjusting device, fixing device and scraping device, fixing device is installed on adjusting device, scraping device is installed on adjusting device;Adjusting device includes bottom plate (1), adjusting nut (2), leveling bolt (3), bearing (4) and support leg (5), the upper end surface of bottom plate (1) four groups of corner is respectively provided with a group of adjusting nut (2), adjusting nut (2) is connected with leveling bolt (3), the lower part of leveling bolt (3) is connected with the upper end surface of support leg (5) by bearing (4);Fixing device includes annular limit seat (6), sliding plate (7), handle slot (8), tension spring (9) and arc fixed plate (10), the upper end surface right part of bottom plate (1) is provided with annular limit seat (6), the upper end surface left part and right part of annular limit seat (6) are respectively provided with a group of rectangular sliding slot, two groups of sliding slot are extended to the outside of annular limit seat (6), a group of sliding plate (7) is placed in two groups of sliding slot respectively, handle slot (8) is set on the upper end surface outside of sliding plate (7), handle slot (8) is connected between the outside of annular limit seat (6) by tension spring (9), arc fixed plate (10) is installed on the upper end surface inside of sliding plate (7);Scraping device includes horizontal electric cylinder (14), vertical electric cylinder (15), rotating shaft seat (16), fastening bolt (17), scraper frame (18) and scraper (19), the upper end surface left part of bottom plate (1) is installed with horizontal electric cylinder (14) by support, the moving end of horizontal electric cylinder (14) is connected with vertical electric cylinder (15), the moving end of vertical electric cylinder (15) is installed with rotating shaft seat (16), fastening bolt (17) is installed on the outside of rotating shaft seat (16), the end of fastening bolt (17) extends to the inside of rotating shaft seat (16) and can be contacted with the rotating shaft side of rotating shaft seat (16), rotating shaft seat (16) is connected with scraper frame (18) on the rotating shaft side, and scraper frame (18) is provided with mounting groove in lower part, and scraper (19) is fixed in mounting groove by bolt.

2. An immunomodulatory cell scraping assembly as in claim 1, wherein, Still including drainage hole (11), arc water retaining edge (12) and drainage groove (13), the left side and the right side of annular limit seat (6) are respectively provided with a group of arc water retaining edge (12), and the two groups of arc water retaining edge (12) are located at the outside of sliding slot, drainage hole (11) is arranged on the inner lower end surface of arc water retaining edge (12), and drainage groove (13) is arranged in rectangular sliding slot.

3. An immunomodulatory cell scraping assembly according to claim 2, wherein, Still including limit electric cylinder (20), pressure switch (21) and rubber contact (22), limit electric cylinder (20) is arranged on the right end surface of vertical electric cylinder (15), and rubber contact (22) is connected on the moving end of limit electric cylinder (20) by pressure switch (21).

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