Multi-gas culture device for microbial culture
By setting up a cross-shaped partition and using a motor drive inside the incubator to shake the culture cups inside the incubator, the problems of experimental monotony and slow gas exchange caused by the single chamber of the incubator are solved, and the repeatability and speed of multi-gas culture are achieved.
Patent Information
- Application Number
- CN202520397798.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2026-02-13
- Estimated Expiration
- 2035-03-10
AI Technical Summary
In the prior art, the single chamber of the multi-gas culture device leads to single culture and experimental results, and the quiescent state of microorganisms reduces the rate of contact and exchange with the gas.
A multi-gas culture device is designed, which divides the incubator into multiple chambers by setting a cross-shaped partition inside the incubator, and uses a motor to drive the support plate and culture cup to reciprocate and shake, thereby promoting gas exchange.
This technology enables the reproducibility and accuracy of parallel experimental results in multi-gas culture devices, improves the culture speed, and promotes the growth and metabolism of microorganisms.
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Figure CN223906838U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to microorganism culture technical field, concretely for a kind of multi-gas culture device for microorganism culture. BACKGROUND
[0002] Microorganisms play an irreplaceable role in many fields. In the medical field, microorganism culture helps to study the characteristics of pathogens, which is crucial for disease diagnosis, treatment and vaccine development. In the food industry, microorganism culture is used in fermentation processes, such as the production of yogurt, bread, beer, etc., which relies on the fermentation of specific microorganisms. In environmental science, microorganism culture can be used to study the degradation ability of microorganisms on pollutants to develop effective environmental remediation technologies. Since microorganisms will produce different reactions to different types and concentrations of gases, a multi-gas culture device is needed to culture microorganisms.
[0003] Common multi-gas culture devices include a box, a gas pump, and a culture dish. When using, microorganisms are placed in the culture dish, and different gases are introduced into the box through the gas pump to culture the microorganisms. However, this method has the following problems: the single chamber of the box not only leads to single culture and experimental results, but also reduces the speed of microorganisms in contact with gases in a static state, which cannot meet the working requirements of microorganism culture. Therefore, a multi-gas culture device for microorganism culture is proposed. UTILITY MODEL CONTENT
[0004] (I) Technical problem solved
[0005] To solve the technical problem of single chamber of the box not only leading to single culture and experimental results, and reducing the speed of microorganisms in contact with gases in a static state, the utility model provides a multi-gas culture device for microorganism culture.
[0006] (II) Technical solution
[0007] To achieve the above purpose, the utility model provides the following technical scheme: a multi-gas culture device for microorganism culture, comprising:
[0008] A bottom plate is provided with a culture box on the top, a cross partition is connected to the inside of the top of the bottom plate, and a valve core is installed on the top of the culture box.
[0009] A glass cover plate is hinged to the outside of the culture box corresponding to the position of the cross partition cavity. Motors are installed on the top of the bottom plate at the position of the cross partition cavity. Supports are connected to the left and right sides of the top of the bottom plate.
[0010] The square frame is installed on the inner side upper part of the support through a bearing, a rotating shaft is arranged at the inner middle position of the square frame, and a shaft column is installed at the inner end of the rotating shaft, and a supporting plate is coaxially arranged at the top end of the shaft column;
[0011] The driving block is coaxially connected at the top end of the rotor of the motor, a connecting shaft is coaxially installed at the bottom end of the shaft column, and the bottom end of the connecting shaft is connected with the corresponding position of the driving block, a limiting clamping seat is installed at the top right side of the supporting plate, a culture cup is inserted at the inner side of the limiting clamping seat, a cup cover is sleeved at the top of the culture cup, and an air nozzle is arranged at the top of the cup cover.
[0012] Preferably, the outer part of the glass cover plate is provided with a handle, and the outer periphery of the glass cover plate is provided with a sealing gasket, which improves the sealing performance of the inside of the incubator, and the number of the air valve cores on one side is 2-6 groups.
[0013] Preferably, the outer end of the driving block is inclined, and the four corners of the square frame are round.
[0014] Preferably, the top left side of the supporting plate is connected with a mounting plate, and the inner sides of the two sides of the mounting plate are inserted with cross bars, and the left end of the cross bar is provided with a clamping plate.
[0015] Preferably, the inner sides of the clamping plate and the limiting clamping seat are arc-shaped, and springs are sleeved at the positions between the mounting plate and the clamping plate on the outer part of the cross bar. By adding the clamping plate and the spring, the culture cup can be clamped after being placed, so as to avoid falling when shaking.
[0016] Preferably, the two ends of the spring are respectively connected with the corresponding positions of the inner sides of the mounting plate and the clamping plate, and the left end of the cross bar is coaxially provided with a limiting disc.
[0017] (Three) beneficial effects
[0018] Compared with the prior art, the utility model provides a kind of multiple gas culture device for microorganism culture, with the following beneficial effects:
[0019] The multiple gas culture device for microorganism culture, by the cross partition that is additionally provided, the inside of the incubator is divided into multiple chambers, so that parallel experiments can be carried out, ensuring the repeatability and accuracy of experimental results, and improving the speed of cultivation. Meanwhile, the rotation of the driving block can be driven by the motor, so that the movement of the shaft column and the supporting plate can be driven by the connecting shaft. Under the cooperation of the direction frame and the rotating shaft, the supporting plate and the culture cup are reciprocally shaken. This not only prevents the nutrients from depositing at the bottom of the culture cup, but also forms micro fluctuations and convection through the shaking of the culture cup, constantly updates the surface of the culture cup, promotes the exchange of gas, and thus promotes the better growth and metabolism of microorganisms, improving the practicability of the multiple gas culture device.BRIEF DESCRIPTION OF DRAWINGS BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1 It is the structure schematic diagram of the utility model;
[0021] Figure 2 It is the structure schematic diagram of the utility model bottom plate top structure;
[0022] Figure 3 It is the structure schematic diagram of the utility model support and square frame structure;
[0023] Figure 4 It is the structure schematic diagram of the utility model bearing plate upside structure;
[0024] Figure 5 It is the structure schematic diagram of the utility model connecting shaft and starting block structure;
[0025] Figure 6 It is the structure schematic diagram of the utility model bearing plate top structure.
[0026] In the drawing: 1, bottom plate; 2, incubator; 3, valve core; 4, glass cover plate; 5, cross partition; 6, support; 7, motor; 8, square frame; 9, shaft column; 10, bearing plate; 11, rotating shaft; 12, connecting shaft; 13, driving block; 14, limit seat; 15, clamping plate; 16, culture cup; 17, cup cover; 18, mounting plate; 19, cross bar; 20, spring; 21, limit disc; 22, air cock. DETAILED DESCRIPTION
[0027] The technical scheme in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the scope of protection of the utility model.
[0028] The utility model provides a kind of technical scheme, a kind of multi-gas culture device for microorganism culture, including bottom plate 1, incubator 2, valve core 3, glass cover plate 4, cross partition 5, support 6, motor 7, square frame 8, shaft column 9, bearing plate 10, rotating shaft 11, connecting shaft 12, driving block 13, limit seat 14, clamping plate 15, culture cup 16, cup cover 17, mounting plate 18, cross bar 19, spring 20, limit disc 21 and air cock 22:
[0029] Please refer to Figure 1 , the top of bottom plate 1 is equipped with incubator 2, please refer to Figure 2 , the top inboard of bottom plate 1 is connected with cross partition 5, please refer to Figure 1 , the top of incubator 2 is equipped with valve core 3;
[0030] Glass cover plate 4, hinged in the external of incubator 2 and cross partition plate 5 cavity corresponding position, the outer part of glass cover plate 4 is installed with handle, the outer periphery of glass cover plate 4 is clamped with sealing gasket, the number of single edge valve core 3 is 2-6 groups, please refer to Figure 2 , the top of bottom plate 1 is installed with motor 7 in the middle part of cross partition plate 5 cavity, the top of bottom plate 1 is connected with support 6 in the left and right sides of motor 7;
[0031] Please refer to Figure 3 And Figure 4 , square frame 8 is installed on the inner side of support 6 through bearing, the inside of square frame 8 is installed with rotating shaft 11 in the middle position, and the inner end of rotating shaft 11 is installed with shaft column 9, the top end of shaft column 9 is coaxially installed with bearing plate 10;
[0032] Please refer to Figure 5 , driving block 13 is coaxially connected with the top end of rotor of motor 7, the bottom end of shaft column 9 is coaxially installed with connecting shaft 12, and the bottom end of connecting shaft 12 is connected with the corresponding position of driving block 13, please refer to Figure 6 , the top right side of bearing plate 10 is installed with limiting card seat 14, the inner side of limiting card seat 14 is inserted with culture cup 16, the top of culture cup 16 is sleeved with cup cover 17, and the top of cup cover 17 is installed with air nozzle 22, the outer end of driving block 13 is inclined, the four corners of square frame 8 are round, the diameter of bearing plate 10 is greater than the width of square frame 8, the inside of incubator 2 is divided into multiple chambers through the added cross partition plate 5, so that parallel experiment can be carried out, the repeatability and accuracy of experimental results are guaranteed, and the culture speed is improved, at the same time, the rotation of driving block 13 can be driven by motor 7, so that the movement of shaft column 9 and bearing plate 10 can be driven by connecting shaft 12, and under the cooperation of square frame 8 and rotating shaft 11, bearing plate 10 and culture cup 16 can be reciprocatingly shaken, which not only can prevent the sedimentation of nutrients at the bottom of culture cup 16, but also can form micro fluctuations and convection through the shaking of culture cup 16, so that the surface of culture cup 16 is constantly renewed, the gas exchange is promoted, and the practicability of multi-gas culture device is improved;
[0033] The top left side of bearing plate 10 is connected with mounting plate 18, the inner sides of mounting plate 18 are inserted with cross rod 19, the left ends of cross rod 19 are installed with clamping plate 15, the inner sides of clamping plate 15 and limiting card seat 14 are arc-shaped, the outer parts of cross rod 19 are sleeved with spring 20 between mounting plate 18 and clamping plate 15, the two ends of spring 20 are respectively connected with the corresponding positions of inner sides of mounting plate 18 and clamping plate 15, the left ends of cross rod 19 are coaxially installed with limiting disc 21.
[0034] The cross partition 5 is arranged to divide the incubator 2 into multiple chambers, so that parallel experiments can be carried out, the repeatability and accuracy of the experimental results are ensured, the culture speed is improved, the rotation of the driving block 13 is driven by the motor 7, the movement of the shaft column 9 and the supporting plate 10 is driven by the connecting shaft 12, the reciprocating shaking of the supporting plate 10 and the culture cup 16 is realized under the cooperation of the square frame 8 and the rotating shaft 11, the deposition of nutrients at the bottom of the culture cup 16 is prevented, the shaking of the culture cup 16 forms micro fluctuations and convection, the surface of the culture cup 16 is constantly renewed, the gas exchange is promoted, the growth and metabolism of microorganisms are promoted, and the practicability of the multi-gas culture device is improved.
[0035] It should be noted that, in this document, the terms such as first and second are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.
[0036] Although the embodiments of the present application have been shown and described, it is understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.
Claims
1. A multi-gas culture device for microbial cultivation, characterized by, The utility model relates to a culture box, which comprises a bottom plate (1) with a culture box (2) mounted on the top of the bottom plate (1), a cross partition (5) connected to the inner side of the top of the bottom plate (1), a valve core (3) mounted on the top of the culture box (2), a glass cover plate (4) hinged to the outside of the culture box (2) at a position corresponding to the cavity of the cross partition (5), a motor (7) mounted on the top of the bottom plate (1) at a position in the middle of the cavity of the cross partition (5), a support (6) connected to the top of the bottom plate (1) at positions on the left and right sides of the motor (7), a square frame (8) mounted on the inner side of the support (6) at the upper part thereof, a rotating shaft (11) mounted in the middle of the interior of the square frame (8), a shaft column (9) mounted on the inner end of the rotating shaft (11), a bearing plate (10) coaxially mounted on the top end of the shaft column (9), a driving block (13) coaxially connected to the top end of the rotor of the motor (7), a connecting shaft (12) coaxially mounted on the bottom end of the shaft column (9) and connected to the corresponding position of the driving block (13), a limiting clamping seat (14) mounted on the top right side of the bearing plate (10), a culture cup (16) inserted into the inner side of the limiting clamping seat (14), a cup cover (17) sleeved on the top of the culture cup (16), and an air nozzle (22) mounted on the top of the cup cover (17). The outer part of the glass cover plate (4) is provided with a handle, and the outer periphery of the glass cover plate (4) is provided with a sealing gasket. The outer end of the driving block (13) is inclined, the four corners of the square frame (8) are rounded, and the diameter of the bearing plate (10) is greater than the width of the square frame (8). The top left side of the bearing plate (10) is connected with a mounting plate (18), the inner sides of the two sides of the mounting plate (18) are inserted with a cross rod (19), and the left end of the cross rod (19) is provided with a clamping plate (15). The inner sides of the clamping plate (15) and the limiting clamping seat (14) are arc-shaped, and the outer part of the cross rod (19) is sleeved with a spring (20) between the mounting plate (18) and the clamping plate (15).
2. The multi-gas culture apparatus for microbial cultivation according to claim 1, wherein: The two ends of the spring (20) are connected with the corresponding positions of the inner sides of the mounting plate (18) and the clamping plate (15), and the left end of the cross rod (19) is coaxially provided with a limiting disc (21).
3. The multi-gas culture apparatus for microbial cultivation according to claim 1, wherein: 4. The multi-gas culture apparatus for microbial cultivation according to claim 1, wherein: 5. A multi-gas culture apparatus for microbial cultivation according to claim 4, wherein: 6. A multi-gas culture apparatus for microbial cultivation according to claim 5, wherein: