Liquid path system of glycosylated hemoglobin analyzer

Abstract

The utility model relates to a liquid path system of a glycosylated hemoglobin analyzer. The liquid path system comprises a first eluent barrel, a high-pressure pump, a sampling valve, a chromatographic column and a detector which are sequentially connected through a pipeline, the sample injection valve consists of a three-way joint and a high-pressure one-way valve; three ends of the three-way joint are respectively connected with outlets of the high-pressure pump, the chromatographic column and the high-pressure one-way valve; an inlet of the high-pressure one-way valve is connected with a sample injection unit, and the sample injection unit is used for pumping a sample to be detected into the sample injection valve. A high-pressure-resistant sampling valve is replaced by the sample injection valve consisting of the three-way joint and the high-pressure one-way valve, so that a to-be-detected sample is supplied into the chromatographic column, and the cost is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection equipment technical field, especially relate to a liquid path system of glycosylated hemoglobin analyzer. BACKGROUND

[0002] Glycosylated hemoglobin (HbA1c) is a good index for diabetes diagnosis and disease control degree monitoring of diabetes patients. Glycosylated hemoglobin detection has important clinical significance. The main methods for detecting glycosylated hemoglobin in clinic include high performance liquid chromatography (HPLC) method, electrophoresis method, enzyme method and latex turbidimetry method; among them, HPLC method is widely used as the gold standard for glycosylated hemoglobin detection, which provides essential information basis for the diagnosis, treatment and health status of diseases in clinic. The liquid path system is a key component in the glycosylated hemoglobin analyzer.

[0003] The Chinese patent with publication number CN206431150U provides a kind of fully automatic glycosylated hemoglobin analyzer, including: sample injection puncture ware, liquid path analysis system, electric control system, the liquid path analysis system includes liquid path switching unit, dilution analysis unit, cleaning unit;The liquid path switching unit includes injection pump and sampling valve;The dilution analysis unit includes mixing tank, high-pressure pump group, micro column unit and detector unit;The cleaning unit includes liquid inlet unit, first waste unit, second waste unit and sampling needle cleaning block.The sampling valve in the liquid path analysis system is connected with high-pressure pump group box micro column unit respectively, and the sampling valve is in high-pressure pipeline, so it is necessary to use high-pressure sampling valve, thereby it is not conducive to reduce cost.

[0004] Therefore, the prior art has great room for improvement. UTILITY MODEL CONTENT

[0005] The utility model aims at making up for the deficiency of prior art, and provides a kind of liquid path system of glycosylated hemoglobin analyzer.

[0006] To achieve the above object, the utility model realizes by the following technical scheme:

[0007] The utility model provides a kind of liquid path system of glycosylated hemoglobin analyzer, including first eluent bucket, high-pressure pump, sample injection valve, chromatographic column and detector connected by pipeline in proper order;The sample injection valve is composed of three-way joint and high-pressure check valve, and the three ends of the three-way joint are connected with the outlet of high-pressure pump, chromatographic column and high-pressure check valve respectively;The inlet of high-pressure check valve is connected with sample injection unit, and the sample injection unit is used to pump into the sample injection valve for the sample to be measured.

[0008] By using the sample injection valve composed of three-way joint and high-pressure check valve to replace high-pressure sampling valve, it is realized to supply sample to be measured into chromatographic column, and it is conducive to reduce cost.

[0009] According to the above scheme, the sample injection unit comprises a plunger pump, a valve group, a hemolytic agent barrel, a sampling needle and a dilution tank, and the valve group is respectively connected with the plunger pump, the hemolytic agent barrel, the sampling needle and the inlet of the high-pressure one-way valve.

[0010] It can be understood that the pipelines between the high-pressure pump and the detector are high-pressure pipelines, and the pipelines of the sample injection unit are low-pressure pipelines, and the processing of the sample to be measured is completed at low pressure, without the need for high-pressure pipelines, thereby facilitating further cost reduction.

[0011] By controlling the plunger pump and the valve group, a quantitative blood sample and a hemolytic agent can be transferred to the dilution tank to complete the processing and obtain the sample to be measured; then by controlling the plunger pump and the valve group, a quantitative sample to be measured is input from the low-pressure pipeline to the high-pressure pipeline through the sample injection valve, and then is delivered to the chromatographic column through high pressure for chromatography, and finally different components in the sample to be measured are sequentially detected by the detector to realize different optical signal detection.

[0012] In order to accurately control the injection amount of the sample to be measured into the chromatographic column, in some embodiments, the valve group comprises a first three-way valve, a second three-way valve and a third three-way valve, the plunger pump is connected with the common end of the first three-way valve, the normally open end and the normally closed end of the first three-way valve are respectively connected with the hemolytic agent barrel and the common end of the second three-way valve; the normally open end of the second three-way valve is connected with a waste liquid barrel, and the normally closed end of the second three-way valve is connected with the common end of the third three-way valve through a quantitative pipeline; the normally open end and the normally closed end of the third three-way valve are respectively connected with the inlet of the high-pressure one-way valve and the sampling needle.

[0013] Through the above structure, the volume of the sample to be measured entering the chromatographic column each time is kept consistent, and the instrument has good sample injection repeatability. The plunger pump is communicated with the sampling needle through the first three-way valve, the second three-way valve and the third three-way valve. The plunger pump sucks, and the sampling needle sucks the sample to be measured. The sample to be measured sequentially passes through the third three-way valve, the second three-way valve and the first three-way valve to fill the quantitative pipeline. The second three-way valve is closed, and the excess sample to be measured between the plunger pump, the first three-way valve and the third three-way valve is discharged to avoid interference of the residual sample to be measured on subsequent detection. Then the high-pressure pump is paused and depressurized to reduce the pressure of the high-pressure pipeline, the second three-way valve and the third three-way valve are switched, and the plunger pump is controlled to pump the filled sample to be measured in the quantitative pipeline into the sample injection valve. The high-pressure pump is started to pressurize and suck the first eluent in the first eluent barrel, so that the first eluent pushes the sample to be measured into the chromatographic column.

[0014] According to the above scheme, a cleaning swab, a fourth three-way valve and a waste liquid pump are further included, the liquid inlet of the cleaning swab is connected with the normally open end of the second three-way valve, the normally open end and the normally closed end of the fourth three-way valve are respectively connected with the dilution tank and the liquid outlet of the cleaning swab, and the liquid inlet and the liquid outlet of the waste liquid pump are respectively connected with the common end of the fourth three-way valve and the waste liquid barrel.

[0015] The hemolytic agent in the hemolytic agent barrel can enter the cleaning swab through the first three-way valve and the second three-way valve, and the sampling needle moves up and down in the cleaning swab, so as to complete the cleaning of the outer wall of the sampling needle. The cleaning swab is used not only for cleaning the outer wall of the sampling needle, but also for collecting cleaning waste liquid and excess sample to be tested. By starting the waste liquid pump, the fluid in the cleaning swab is discharged into the waste liquid barrel.

[0016] According to the above scheme, a liquid collection sealing box is further connected between the waste liquid pump and the waste liquid barrel; the liquid collection sealing box is further connected with the high-pressure pump and the pressure stabilizing ring respectively, and the pressure stabilizing ring is further connected with the detector.

[0017] The liquid collection sealing box collects the waste liquid discharged from the cleaning swab, the high-pressure pump and the pressure stabilizing ring, forms a closed space in the liquid collection sealing box, and flushes the collected waste liquid into the waste liquid barrel by using the positive pressure generated by the waste liquid pump, so as to complete the collection and discharge of the waste liquid. By connecting the pressure stabilizing ring with the detector, the pressure stabilizing ring is used to stabilize the pressure in the pipeline, so as to prevent the pressure fluctuation when the high-pressure fluid in the chromatographic column flows to the detector at normal pressure, and affect the detection.

[0018] According to the above scheme, a cleaning joint and a one-way valve are connected to the high-pressure pump, the sampling needle can be inserted into the inlet of the cleaning joint and is in sealed connection with the inlet of the cleaning joint; the liquid inlet of the one-way valve is connected with the high-pressure pump, and the liquid outlet of the one-way valve is connected with the liquid collection sealing box.

[0019] The hemolytic agent enters the high-pressure pump through the inner cavity of the sampling needle, flushes the plunger of the high-pressure pump, and the waste liquid after cleaning flows into the liquid collection sealing box through the one-way valve. The one-way valve functions to prevent the positive pressure in the liquid collection sealing box from interfering with the cleaning of the high-pressure pump. The prior art generally uses a separate cleaning pump for cleaning the high-pressure pump, and the above structure can save a cleaning pump for cleaning the high-pressure pump, which is conducive to further reducing the cost.

[0020] According to the above scheme, a pressure relief valve and a filter are further connected in sequence on the pipeline between the sample injection valve and the chromatographic column.

[0021] A pressure relief valve is arranged on the pipeline between the sample injection valve and the chromatographic column, so that by manually opening the pressure relief valve, air in the pipeline can be discharged, the pressure in the pipeline is reduced, and the pipeline has small resistance when refilling the sample to be tested or switching the eluent.

[0022] The fifth three-way valve has a normally open end and a normally closed end connected with the first eluent tank and the second eluent tank respectively, and a common end connected with the inlet end of the high-pressure pump.

[0023] The first eluent tank and the second eluent tank store first eluent and second eluent with different concentrations respectively, so that the opening and closing of the fifth three-way valve can be controlled to supply the first eluent and the second eluent to the chromatographic column respectively, and the hemoglobin of the sample to be tested is eluted in two gradients, which is beneficial to accelerate the elution speed.

[0024] According to the above scheme, the fifth three-way valve is provided with a bubble sensor between the high-pressure pump.

[0025] The bubble sensor is used to detect whether air bubbles caused by failure are sucked into the pipeline, so that the user can be timely warned, and the double-head high-pressure pump can be effectively prevented from being damaged.

[0026] According to the above scheme, the high-pressure pump is a double-head high-pressure plunger pump.

[0027] The double-head high-pressure plunger pump is a plunger pump with two plunger pressurizing chambers, which can provide a maximum pressure of 40MPa, and greatly reduces pressure fluctuation compared with a single-head high-pressure plunger pump, so that detection is more stable and accurate.

[0028] The beneficial effects of the utility model lie in:

[0029] The liquid path system of the glycated hemoglobin analyzer is set, so that a sampling valve with high pressure resistance is replaced by a sample injection valve composed of a three-way joint and a high-pressure check valve, the sample to be tested is supplied into the chromatographic column, and cost is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 is a structural schematic view of the liquid path system of the glycated hemoglobin analyzer.

[0031] In the diagram: 10. Plunger pump; 11. First three-way valve; 111. Hemolysin tank; 12. Second three-way valve; 13. Third three-way valve; 14. Sampling needle; 20. Cleaning swab; 21. Dilution tank; 22. Fourth three-way valve; 23. Waste liquid pump; 24. Collection sealing box; 25. Waste liquid tank; 26. Cleaning connector; 27. Check valve; 30. First eluent tank; 31. Fifth three-way valve; 311. Second eluent tank; 32. Bubble sensor; 33. High-pressure pump; 34. Injection valve; 341. Three-way connector; 342. High-pressure check valve; 35. Pressure relief valve; 36. Filter; 37. Chromatographic column; 38. Detector; 39. Pressure stabilizing ring. Detailed Implementation

[0032] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0033] like Figure 1 As shown, this utility model provides a liquid circuit system for a glycated hemoglobin analyzer, including a first eluent tank 30, a high-pressure pump 33, an injection valve 34, a chromatographic column 37, and a detector 38 connected in sequence by pipelines; the injection valve 34 consists of a three-way connector 341 and a high-pressure one-way valve 342, the three ends of the three-way connector 341 being connected to the outlets of the high-pressure pump 33, the chromatographic column 37, and the high-pressure one-way valve 342 respectively; the inlet of the high-pressure one-way valve 342 is connected to the injection unit, which is used to pump the sample to be tested into the injection valve 34.

[0034] By replacing the high-pressure resistant sampling valve with an injection valve 34 consisting of a three-way connector 341 and a high-pressure one-way valve 342, the sample to be tested can be supplied into the chromatographic column 37, which helps to reduce costs.

[0035] Furthermore, the injection unit includes a plunger pump 10, a valve assembly, a hemolysin tank 111, a sampling needle 14, and a dilution tank 21. The valve assembly is connected to the inlet of the plunger pump 10, the hemolysin tank 111, the sampling needle 14, and the high-pressure one-way valve 342, respectively.

[0036] It is understood that the pipelines between the high-pressure pump 33 and the detector 38 are all high-pressure pipelines, while the pipelines of the sample injection unit are all low-pressure pipelines. The processing of the sample to be tested, including steps such as mixing and dilution, is completed under low pressure, without the need for high-pressure pipelines, which helps to further reduce costs.

[0037] By controlling the plunger pump 10 and the valve group, a quantitative blood sample and a hemolytic agent can be transferred to the dilution tank 21 to complete the processing to obtain a sample to be tested; then by controlling the plunger pump 10, the valve group, a quantitative sample to be tested is input from the low-pressure pipeline to the high-pressure pipeline through the sample valve 34, and then is delivered to the chromatographic column 37 through the high-pressure pipeline for chromatography, and finally different components in the sample to be tested are sequentially detected by the detector 38 to realize different optical signal detection.

[0038] Further, the valve group comprises a first three-way valve 11, a second three-way valve 12 and a third three-way valve 13, the plunger pump 10 is connected with the common end of the first three-way valve 11, the normally open end and the normally closed end of the first three-way valve 11 are respectively connected with the hemolytic agent barrel 111 and the common end of the second three-way valve 12, the normally open end of the second three-way valve 12 is connected with the waste liquid barrel 25, the normally closed end of the second three-way valve 12 is connected with the common end of the third three-way valve 13 through the quantitative pipeline, and the normally open end and the normally closed end of the third three-way valve 13 are respectively connected with the inlet of the high-pressure one-way valve 342 and the sampling needle 14.

[0039] Through the above structure, the sample to be tested can be accurately controlled to enter the chromatographic column 37, and the volume of the sample to be tested entering the chromatographic column 37 each time is kept consistent, so that the instrument has good sample injection repeatability. The plunger pump 10 is communicated with the sampling needle 14 through the first three-way valve 11, the second three-way valve 12 and the third three-way valve 13, the plunger pump 10 sucks, the sampling needle 14 sucks the sample to be tested, and the sample to be tested sequentially passes through the third three-way valve 13, the second three-way valve 12 and the first three-way valve 11 to fill the quantitative pipeline with the sample to be tested; the second three-way valve 12 is closed, the excess sample to be tested between the plunger pump 10, the first three-way valve 11 and the third three-way valve 13 is discharged, and the residual sample to be tested is avoided from interfering with the subsequent detection; then the high-pressure pump 33 is paused and depressurized to reduce the pressure of the high-pressure pipeline, the second three-way valve 12 and the third three-way valve 13 are switched, and the plunger pump 10 is controlled to pump the filled sample to be tested in the quantitative pipeline into the sample valve 34; the high-pressure pump 33 is started to pressurize and suck the first eluent in the first eluent barrel 30, so that the first eluent pushes the sample to be tested into the chromatographic column 37.

[0040] Further, it further comprises a cleaning swab 20, a fourth three-way valve 22 and a waste liquid pump 23, the liquid inlet of the cleaning swab 20 is connected with the normally open end of the second three-way valve 12, the normally open end and the normally closed end of the fourth three-way valve 22 are respectively connected with the dilution tank 21 and the liquid outlet of the cleaning swab 20, and the liquid inlet and the liquid outlet of the waste liquid pump 23 are respectively connected with the common end of the fourth three-way valve 22 and the waste liquid barrel 25.

[0041] The hemolytic agent in the hemolytic agent barrel 111 can enter the cleaning swab 20 through the first three-way valve 11 and the second three-way valve 12, and the sampling needle 14 moves up and down in the cleaning swab 20, so as to complete the cleaning of the outer wall of the sampling needle 14. The cleaning swab 20 is used not only for cleaning the outer wall of the sampling needle 14, but also for collecting cleaning waste liquid and excess sample to be tested. By opening the waste liquid pump 23, the fluid in the cleaning swab 20 is discharged into the waste liquid barrel 25.

[0042] Further, the waste liquid pump 23 and the waste liquid barrel 25 are further connected with the liquid collection sealing box 24; the liquid collection sealing box 24 is further connected with the high-pressure pump 33 and the pressure stabilizing ring 39 respectively, and the pressure stabilizing ring 39 is further connected with the detector 38.

[0043] The liquid collection sealing box 24 collects the waste liquid discharged from the cleaning swab 20, the high-pressure pump 33 and the pressure stabilizing ring 39, forms a closed space in the liquid collection sealing box 24, and uses the positive pressure generated by the waste liquid pump 23 to flush the collected waste liquid into the waste liquid barrel 25, so as to complete the collection and discharge of the waste liquid. By connecting the pressure stabilizing ring 39 with the detector 38, the pressure stabilizing ring 39 is used to stabilize the pressure in the pipeline, so as to prevent the pressure fluctuation when the high-pressure fluid in the chromatographic column 37 flows to the detector 38 under normal pressure, and affect the detection.

[0044] Further, the high-pressure pump 33 is connected with the cleaning joint 26 and the one-way valve 27, the sampling needle 14 can be inserted into the inlet of the cleaning joint 26 and is in sealed connection with the inlet of the cleaning joint 26; the liquid inlet of the one-way valve 27 is connected with the high-pressure pump 33, and the liquid outlet of the one-way valve 27 is connected with the liquid collection sealing box 24.

[0045] The hemolytic agent enters the high-pressure pump 33 through the inner cavity of the sampling needle 14, flushes the plunger of the high-pressure pump 33, and the cleaned waste liquid flows into the liquid collection sealing box 24 through the one-way valve 27. The one-way valve 27 is used to prevent the pressure in the liquid collection sealing box 24 from interfering with the cleaning of the high-pressure pump 33. The prior art generally uses a separate cleaning pump for cleaning the high-pressure pump 33, and the above structure can save a cleaning pump for cleaning the high-pressure pump 33, which is conducive to further reducing the cost.

[0046] Further, the pipeline between the sample inlet valve 34 and the chromatographic column 37 is further connected with the pressure relief valve 35 and the filter 36 in sequence.

[0047] The pressure relief valve 35 is arranged on the pipeline between the sample inlet valve 34 and the chromatographic column 37, so that the air in the pipeline can be discharged by manually opening the pressure relief valve 35, the pressure in the pipeline is reduced, and the resistance of the pipeline is small when the pipeline is refilled with the sample to be tested or switched with the eluent. The filter 36 is arranged before the chromatographic column 37, which is used to filter impurities in the fluid, and is conducive to preventing the chromatographic column 37 from being blocked.

[0048] Further, the first eluent barrel 30 is connected with a fifth three-way valve 31, and the normally open end and the normally closed end of the fifth three-way valve 31 are connected with the first eluent barrel 30 and a second eluent barrel 311 respectively, and the common end of the fifth three-way valve 31 is connected with the inlet end of the high-pressure pump 33.

[0049] The first eluent barrel 30 and the second eluent barrel 311 store first eluent and second eluent with different concentrations respectively, so that the opening and closing of the fifth three-way valve 31 can be controlled to supply the first eluent and the second eluent to the chromatographic column 37 respectively, and then the hemoglobin of the sample to be measured is eluted in two gradients, which is beneficial to accelerate the elution speed.

[0050] Further, the fifth three-way valve 31 is provided with a bubble sensor 32 between the high-pressure pump 33.

[0051] The bubble sensor 32 is used to detect whether there is bubble suction caused by failure in the pipeline, so as to timely alarm the user and effectively prevent the double-head high-pressure pump 33 from being damaged.

[0052] Further, the high-pressure pump 33 is a double-head high-pressure plunger pump.

[0053] The double-head high-pressure plunger pump is a plunger pump 10 with two plunger pressurizing chambers, which can provide a maximum pressure of 40 MPa. Compared with a single-head high-pressure plunger pump 10, the pressure fluctuation is greatly reduced, the detection is more stable, and the detection accuracy is higher.

[0054] The liquid path system of the glycated hemoglobin analyzer is used, first, the high-pressure pump 33 is started to pump the first eluent in the first eluent barrel 30 to flush the chromatographic column 37, the plunger pump 10 sucks the quantitative hemolytic agent, the sampling needle 14 moves to the sampling position, then the first three-way valve 11, the second three-way valve 12 and the third three-way valve 13 are opened, the plunger pump 10 sucks the quantitative sample, then the quantitative hemolytic agent is injected into the dilution tank 21, then the plunger pump 10 is used to suck and spit to mix the sample to be measured; the plunger pump 10 sucks, the sampling needle 14 sucks the sample to be measured in the dilution tank 21, the sample to be measured passes through the sampling needle 14, the third three-way valve 13, the second three-way valve 12 and the first three-way valve 11 in sequence, so that the sample to be measured fills the quantitative pipeline; then the second three-way valve 12 is closed, the fourth three-way valve 22 and the waste liquid pump 23 are opened, the excess sample to be measured between the plunger pump 10, the first three-way valve 11 and the third three-way valve 13 is flushed into the cleaning swab 20, and is sucked into the waste liquid barrel 25; then the high-pressure pump 33 is paused and pressure relief is performed to reduce the pressure of the high-pressure pipeline, the second three-way valve 12 is opened, the plunger pump 10 is controlled to pump the sample to be measured filled in the quantitative pipeline into the sample valve 34 through the normally open port of the third three-way valve 13; then the high-pressure pump 33 is started to pressurize and suck the first eluent in the first eluent barrel 30, so that the first eluent pushes the sample to be measured to flow through the pressure relief valve 35, the filter 36, the chromatographic column 37, the detector 38 and the pressure stabilizing ring 39 in sequence, and finally enters the liquid collection box; the sample to be measured is chromatographically eluted in the chromatographic column 37, different components in the sample to be measured pass through the detector 38 in sequence to realize different optical signal detection, and a curve graph is drawn; after the high-pressure pump 33 is started for a fixed time, the fifth three-way valve 31 is opened to make the second eluent in the second eluent barrel 311 pumped into the chromatographic column 37, so that the hemoglobin in the sample to be measured is eluted by the first eluent and the second eluent in two gradients, until the elution of all hemoglobin components is completed, and finally a complete curve graph is obtained, and the content of the glycated hemoglobin is calculated.

[0055] The above only describes preferred embodiments of the utility model, therefore, equivalent changes or modifications made according to the structure, features and principles described in the utility model patent application range are included in the utility model patent application range.

Claims

1. A liquid path system of a glycated hemoglobin analyzer, comprising a first eluent tank (30), a high-pressure pump (33), a sample injection valve (34), a chromatographic column (37) and a detector (38) connected in sequence by pipelines, characterized in that the sample injection valve (34) is composed of a three-way joint (341) and a high-pressure check valve (342), the three ends of the three-way joint (341) are connected with the high-pressure pump (33), the chromatographic column (37) and the outlet of the high-pressure check valve (342) respectively; the inlet of the high-pressure check valve (342) is connected with a sample injection unit, and the sample injection unit is used for pumping a sample to be tested into the sample injection valve (34). The sample injection unit comprises a plunger pump (10), a valve group, a hemolytic agent tank (111), a sampling needle (14) and a dilution tank (21), and the valve group is connected with the plunger pump (10), the hemolytic agent tank (111), the sampling needle (14) and the inlet of the high-pressure check valve (342) respectively. The valve group comprises a first three-way valve (11), a second three-way valve (12) and a third three-way valve (13), the plunger pump (10) is connected with the common end of the first three-way valve (11), the normally open end and the normally closed end of the first three-way valve (11) are connected with the hemolytic agent tank (111) and the common end of the second three-way valve (12) respectively; 2. The liquid path system of the glycated hemoglobin analyzer according to claim 1, wherein the normally open end of the second three-way valve (12) is connected with a waste liquid tank (25), the normally closed end of the second three-way valve (12) is connected with the common end of the third three-way valve (13) through a constant flow pipeline; 3. The liquid path system of the glycated hemoglobin analyzer according to claim 2, wherein the normally open end and the normally closed end of the third three-way valve (13) are connected with the inlet of the high-pressure check valve (342) and the sampling needle (14) respectively. Further comprising a cleaning swab (20), a fourth three-way valve (22) and a waste liquid pump (23), the liquid inlet of the cleaning swab (20) is connected with the normally open end of the second three-way valve (12), the normally open end and the normally closed end of the fourth three-way valve (22) are connected with the dilution tank (21) and the liquid outlet of the cleaning swab (20) respectively, and the liquid inlet and the liquid outlet of the waste liquid pump (23) are connected with the common end of the fourth three-way valve (22) and the waste liquid tank (25) respectively. A liquid collection sealing box (24) is further connected between the waste liquid pump (23) and the waste liquid tank (25); the liquid collection sealing box (24) is further connected with the high-pressure pump (33) and a pressure stabilizing ring (39), and the pressure stabilizing ring (39) is further connected with the detector (38).

4. The liquid path system of the glycated hemoglobin analyzer according to claim 3, wherein A cleaning joint (26) and a check valve (27) are connected on the high-pressure pump (33), the sampling needle (14) can be inserted into and sealingly connected with the inlet of the cleaning joint (26); 5. The liquid path system of the glycated hemoglobin analyzer according to claim 4, wherein the liquid inlet of the check valve (27) is connected with the high-pressure pump (33), and the liquid outlet of the check valve (27) is connected with the liquid collection sealing box (24).

6. The liquid path system of the glycated hemoglobin analyzer according to claim 5, wherein A pressure relief valve (35) and a filter (36) are further connected in sequence on the pipeline between the sample injection valve (34) and the chromatographic column (37). ​ 7. The liquid path system of the glycated hemoglobin analyzer according to claim 1, wherein ​ 8. The liquid path system of the glycated hemoglobin analyzer according to claim 1, wherein The fifth three-way valve (31) is connected between the first eluent barrel (30) and the high-pressure pump (33), the normally open end and the normally closed end of the fifth three-way valve (31) are connected with the first eluent barrel (30) and the second eluent barrel (311) respectively, and the common end of the fifth three-way valve (31) is connected with the inlet end of the high-pressure pump (33).

9. The liquid path system of the glycated hemoglobin analyzer according to claim 8, wherein The fifth three-way valve (31) is provided with a bubble sensor (32) between the high-pressure pump (33).

10. The liquid path system of the glycated hemoglobin analyzer according to claim 1, wherein The high-pressure pump (33) is a double-head high-pressure plunger pump.

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