Purification device for biological antibody production

Abstract

The utility model relates to the technical field of purification devices, and discloses a purification device for biological antibody production, which comprises a box body, the transverse plate is arranged in the box body; the top end of the purification pipe is connected with a hose at the bottom of the filter box; a clamping assembly is arranged on the transverse plate and comprises a pipe body, the outer side wall of the purification pipe is sleeved with the pipe body, and the top end of the pipe body is connected with the transverse plate; the groove body is formed in the outer side wall of the pipe body; the clamping blocks are arranged in the groove body, and the top ends of the clamping blocks are rotationally connected with the inner side wall of the groove body. According to the purification device, the clamping assembly utilizes the rotation of the threaded rod I to push the ring body to extrude the clamping block, so that the clamping block rotates to clamp the purification pipe, and compared with an existing device, the purification device is simpler and more convenient to operate. According to the purification device, the height of the top plate can be adjusted through the supporting assembly according to the length of the purification pipe, so that the purification pipe can be connected with a hose at the bottom of the filter box.

Description

Technical Field

[0001] This utility model relates to the field of purification device technology, specifically a purification device for the production of biological antibodies. Background Technology

[0002] Antibodies are protective proteins produced by the body in response to antigen stimulation. They are large, Y-shaped proteins secreted by plasma cells and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. They are found only in the blood and other bodily fluids of vertebrates, and on the cell membrane surface of their B cells. Antibodies recognize a unique characteristic of a specific foreign substance; this foreign target is called an antigen. During the production of biological antibodies, impurities are filtered out to purify them.

[0003] Chinese utility model CN218665851U discloses a novel purification device for protein antibodies, which designs a structure to restrict the purification tubes. The principle is that an adjustment component is set on a disc. When purification tubes of different specifications are placed on the disc, a matching insert ring is inserted into the mounting base. The insert ring is engaged by a locking block and a locking slot. After the purification tube is inserted, the insert ring can restrict the shaking of the purification tube.

[0004] However, existing devices are cumbersome to use, the slots are small and difficult to operate, and a suitable connecting ring is required, making them inconvenient to use. Therefore, a purification tube clamping mechanism was designed to address the above-mentioned problems and facilitate operation. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] To address the shortcomings of existing technologies, this invention provides a purification device for the production of biological antibodies, which has the advantage of convenient operation and solves the problem of cumbersome operation of existing devices.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, this utility model provides the following technical solution: a purification device for biological antibody production, comprising: a housing with a filter box inside; a horizontal plate disposed inside the housing; a purification tube inserted into a groove in the horizontal plate, with its top end connected to a flexible tube at the bottom of the filter box; a clamping assembly disposed on the horizontal plate, the clamping assembly comprising: a tube sleeved on the outer wall of the purification tube, with its top end connected to the horizontal plate; a groove formed on the outer wall of the tube; a clamping block disposed inside the groove, with its top end rotatably connected to the inner wall of the groove; a ring sleeved on the outer wall of the tube; and a threaded rod, the bottom end of which penetrates the horizontal plate and is rotatably connected to the ring and threadedly connected to the horizontal plate.

[0009] In some embodiments, the clamping assembly further includes a knob disposed at the top of the threaded rod.

[0010] In some embodiments, the clamping assembly further includes: the bottom end of the upright extends through the horizontal plate and is connected to the ring body.

[0011] In some embodiments, the clamping assembly further includes a stop block disposed at the top of the upright.

[0012] In some embodiments, the clamping assembly further includes an arcuate plate disposed on the outer side wall of the clamping block.

[0013] In some embodiments, a support assembly is provided at the bottom of the housing, the support assembly comprising: an internally threaded tube rotatably disposed on the inner sidewall of the bottom of the housing; a second threaded rod disposed inside the internally threaded tube and threadedly connected to the internally threaded tube; a rectangular rod disposed inside the second threaded rod and having its bottom end passing through the second threaded rod and connected to the housing; and a top plate disposed at the top of the second threaded rod.

[0014] In some embodiments, the support assembly further includes a baffle disposed at the top of the rectangular rod.

[0015] In some embodiments, the support assembly further includes: multiple levers, all disposed on the outer side wall of the internally threaded tube.

[0016] (III) Beneficial Effects

[0017] Compared with the prior art, this utility model provides a purification device for the production of biological antibodies, which has the following beneficial effects:

[0018] 1. This purification device uses a clamping assembly to use a threaded rod to rotate and push the ring body to squeeze the clamping block, which can clamp the purification tube. Compared with existing devices, it is simpler and more convenient to operate.

[0019] 2. This purification device, through the support assembly, allows the height of the top plate to be adjusted according to the length of the purification tube, so that the purification tube can be connected to the hose at the bottom of the filter box. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the connection between the horizontal plate and the clamping assembly of this utility model;

[0022] Figure 3 This is a schematic diagram of the structure of the present invention where the ring body and the tube body are separated;

[0023] Figure 4This is a schematic diagram of the internal structure of the threaded rod II of this utility model.

[0024] In the diagram: 11. Box body; 12. Filter box; 13. Horizontal plate; 14. Purification tube;

[0025] 2. Clamping assembly; 21. Tube body; 22. Groove body; 23. Clamping block; 24. Ring body; 25. Threaded rod one; 26. Rotary knob; 27. Vertical rod; 28. Stop block; 29. ​​Arc plate;

[0026] 3. Support assembly; 31. Internally threaded tube; 32. Threaded rod II; 33. Rectangular rod; 34. Top plate; 35. Baffle; 36. Lever. Detailed Implementation

[0027] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0028] It should be noted that all directional indications in the embodiments of this application are only used to explain the relative positional relationship and movement of each component in a specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0029] In this application, unless otherwise expressly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0030] Furthermore, the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. If the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed in this application.

[0031] Antibodies are protective proteins produced by the body in response to antigen stimulation. They are large, Y-shaped proteins secreted by plasma cells and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. They are found only in the blood and other bodily fluids of vertebrates, and on the cell membrane surface of their B cells. Antibodies recognize a unique characteristic of a specific foreign substance; this foreign target is called an antigen. During the production of biological antibodies, impurities are filtered out to purify them.

[0032] In related technologies, an adjustment component is set on the disc. When purification tubes of different specifications are placed on the disc, a matching insert ring is inserted into the mounting base. The insert ring engages with a locking block and a locking slot, and then the purification tube is inserted. The insert ring can limit the shaking of the purification tube. However, the existing device is relatively cumbersome to use. The locking slot is small and difficult to operate. In addition, it is necessary to find a suitable insert ring for connection, which is not convenient to use.

[0033] To address some of the problems in related technologies, this application provides a purification device for biological antibody production. When it is necessary to clamp the purification tube 14, the purification tube 14 is first inserted into the horizontal plate 13. Then, by rotating the threaded rod 25, and because the threaded rod 25 is threadedly connected to the horizontal plate 13 and the ring 24 is restricted from rotating due to being fitted onto the outer wall of the tube 21, the horizontal plate 13 moves downwards while the threaded rod 25 rotates, pushing the ring 24 downwards. This causes the ring 24 to squeeze the clamping block 23, which rotates inside the tank 22 and clamps the purification tube 14 on one side, maintaining stability.

[0034] This application is described below with reference to the accompanying drawings and specific embodiments:

[0035] Combination Figures 1-4 This application provides a purification device for biological antibody production, comprising: a housing 11 with a filter box 12 disposed inside; a horizontal plate 13 disposed inside the housing 11; a purification tube 14 inserted into a through groove of the horizontal plate 13, with its top end connected to a flexible tube at the bottom of the filter box 12; a clamping assembly 2 disposed on the horizontal plate 13, the clamping assembly 2 comprising: a tube body 21 sleeved on the outer wall of the purification tube 14, with its top end connected to the horizontal plate 13; a groove 22 formed on the outer wall of the tube body 21; a clamping block 23 disposed inside the groove 22, with its top end rotatably connected to the inner wall of the groove 22; a ring body 24 sleeved on the outer wall of the tube body 21; and a threaded rod 25, the bottom end of which penetrates the horizontal plate 13 and is rotatably connected to the ring body 24 and threadedly connected to the horizontal plate 13.

[0036] Specifically, the purpose of filter box 12 is to separate the culture supernatant from cells and cell debris. Purification tube 14, also called a purification column, operates primarily based on ion exchange and adsorption mechanisms. The purification column is filled with ion exchange resins, which have specific chemical groups that can exchange ions with those in the water. Specifically, cations (such as Na+) are transferred to the resin, while H+ ions from the resin enter the water, thus achieving ion replacement. Similarly, anions (such as Cl-) are replaced by OH- ions from the resin, generating H2O. This ion exchange process allows the purification column to effectively remove ions from the water, thereby achieving purification.

[0037] When it is necessary to clamp the purification tube 14 during use, first insert the purification tube 14 into the horizontal plate 13. Then, by rotating the threaded rod 25, because the threaded rod 25 is threadedly connected to the horizontal plate 13 and the ring 24 is restricted from rotating due to being fitted on the outer wall of the tube body 21, the horizontal plate 13 moves downwards while the threaded rod 25 rotates, pushing the ring 24 downwards. This causes the ring 24 to squeeze the clamping block 23, which rotates inside the tank 22 and clamps the purification tube 14 on one side, keeping it stable.

[0038] In some embodiments, the clamping assembly 2 further includes a knob 26 disposed on the top of the threaded rod 25.

[0039] When in use, the design of the knob 26 makes it easy to grip and control the rotation of the threaded rod 25. At the same time, the grooves on the surface of the knob 26 can increase friction and prevent slippage.

[0040] In some embodiments, the clamping assembly 2 further includes: the bottom end of the upright 27 passes through the horizontal plate 13 and is connected to the ring 24.

[0041] When in use, the design of the upright 27 can stabilize the ring 24 and allow it to move downwards steadily.

[0042] In some embodiments, the clamping assembly 2 further includes a stop 28 disposed on the top of the upright 27.

[0043] The design of the stop 28 during use ensures that it will not detach from the clamp 23 when the ring 24 moves downward.

[0044] In some embodiments, the clamping assembly 2 further includes an arc-shaped plate 29 disposed on the outer side wall of the clamping block 23.

[0045] When in use, the arc-shaped plate 29 is designed to fit better against the outer wall of the purification tube 14, thereby better clamping the purification tube 14.

[0046] In some embodiments, a support assembly 3 is provided at the bottom of the housing 11. The support assembly 3 includes: an internally threaded tube 31 rotatably disposed on the inner side wall of the bottom of the housing 11; a second threaded rod 32 disposed inside the internally threaded tube 31 and threadedly connected to the internally threaded tube 31; a rectangular rod 33 disposed inside the second threaded rod 32 and with its bottom end passing through the second threaded rod 32 and connected to the housing 11; and a top plate 34 disposed on the top of the second threaded rod 34.

[0047] When the top of the purification tube 14 cannot reach the hose at the bottom of the filter box 12 during use, the internal threaded tube 31 can be rotated. While rotating, it is threadedly connected to the second threaded rod 32. The second threaded rod 32 is restricted from rotating by the rectangular rod 33. Therefore, when the internal threaded tube 31 rotates, it can push the top plate 34 to move upward to support the purification tube 14.

[0048] In some embodiments, the support assembly 3 further includes a baffle 35 disposed on the top of the rectangular rod 33.

[0049] The design of the baffle 35 during use ensures that the threaded rod 32 will not detach from the internal threaded tube 31 when it rises.

[0050] In some embodiments, the support assembly 3 further includes: multiple levers 36, all disposed on the outer side wall of the internally threaded tube 31.

[0051] When in use, the lever 36 is designed to facilitate the rotation of the internally threaded tube 31.

[0052] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," and "some examples" indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. In addition, those skilled in the art can combine and integrate the different embodiments or examples described in this specification.

[0053] Furthermore, the technical solutions of the various embodiments can be combined with each other, but only if they are based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0054] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A purification apparatus for the production of biological antibodies, comprising: The housing (11) contains a filter box (12). A horizontal plate (13) is disposed inside the box body (11); The purification tube (14) is inserted into the through groove of the horizontal plate (13), and its top end is connected to the hose at the bottom of the filter box (12); The feature is that a clamping assembly (2) is provided on the horizontal plate (13), and the clamping assembly (2) includes: The tube body (21) is fitted onto the outer wall of the purification tube (14) and its top end is connected to the horizontal plate (13); The groove (22) is formed on the outer wall of the tube (21); A clamping block (23) is disposed inside the groove (22) and its top end is rotatably connected to the inner sidewall of the groove (22); The ring (24) is fitted onto the outer wall of the tube (21); The threaded rod (25) has its bottom end passing through the horizontal plate (13) and is rotatably connected to the ring (24) and threadedly connected to the horizontal plate (13).

2. The purification apparatus for producing biological antibodies according to claim 1, characterized in that: The clamping assembly (2) further includes: A knob (26) is located at the top of the threaded rod (25).

3. The purification apparatus for producing biological antibodies according to claim 1, characterized in that: The clamping assembly (2) further includes: The bottom end of the upright (27) passes through the horizontal plate (13) and is connected to the ring (24).

4. The purification apparatus for producing biological antibodies according to claim 3, characterized in that: The clamping assembly (2) further includes: A stop (28) is provided on the top of the upright (27).

5. The purification apparatus for producing biological antibodies according to claim 1, characterized in that: The clamping assembly (2) further includes: An arc-shaped plate (29) is disposed on the outer wall of the clamping block (23).

6. The purification apparatus for producing biological antibodies according to claim 1, characterized in that: The bottom of the housing (11) is provided with a support assembly (3), the support assembly (3) including: An internally threaded tube (31) is rotatably mounted on the bottom inner wall of the housing (11); Threaded rod 2 (32) is disposed inside the internal threaded tube (31) and threadedly connected to the internal threaded tube (31); A rectangular rod (33) is disposed inside the threaded rod (32), and its bottom end passes through the threaded rod (32) and is connected to the box (11); The top plate (34) is disposed on the top of the threaded rod (32).

7. The purification apparatus for producing biological antibodies according to claim 6, characterized in that: The support component (3) also includes: A baffle (35) is disposed on the top of the rectangular rod (33).

8. The purification apparatus for producing biological antibodies according to claim 6, characterized in that: The support component (3) also includes: Multiple levers (36) are provided, all of which are located on the outer side wall of the internally threaded tube (31).

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