Exosome biological purification column and clamp

Abstract

The utility model discloses an exosome biological purification column and a clamp, and relates to the field of exosome purification, the exosome biological purification column comprises a sealing cover movably mounted at the top of a column body, a guide pipe is fixedly connected to the top of the sealing cover, an adjusting assembly is arranged in the guide pipe, and the adjusting assembly comprises a ball movably mounted in the guide pipe; the center of the ball body is provided with a circulation opening equal to the diameter of an inner hole of the catheter, one side of the surface of the ball body is fixedly connected with a connecting rod with the outer end movably penetrating through the side wall of the catheter and fixedly connected with an adjusting knob, and the connecting rod is used for driving the ball body to rotate in the catheter so as to change the sectional area of a catheter channel; and a clamping block is movably mounted on the surface of the connecting rod, and is clamped and fixed with the guide pipe. In conclusion, according to the device, by calculating the number of the clamping blocks clamped into the clamping grooves, the rotating angle of the connecting rod can be accurately known, and the design is simple and effective and has very high precision and stability.

Description

Technical Field

[0001] This application relates to the field of exosome purification, and in particular to an exosome biopurification column and clamp. Background Technology

[0002] A purification column is an experimental device used for the separation and purification of biomolecules. It typically consists of a column, packing material, filter membrane, and tubing. Its main function is to separate target biomolecules from impurities and other biomolecules in a sample, thereby improving the purity and activity of the biomolecules. It can be used for purification treatment before food safety testing.

[0003] For example, the Chinese patent with authorization announcement number CN220294169U discloses a sample pretreatment purification column for rapid food safety detection, but it still has the following shortcomings in practical use:

[0004] Turning the adjusting nut indirectly drives the sector plates on both sides to rotate, thereby changing the angle of the sector plates and altering the gap between them and the guide tube. The adjustment can only be made by feeling, and it is impossible to know exactly how much the sector plates have rotated. Utility Model Content

[0005] To address the issue of not being able to accurately determine the angle by which the sector plate has rotated, this application provides an exosome biological purification column and clamp.

[0006] The exosome biopurification column and clamp provided in this application adopt the following technical solution:

[0007] An exosome biological purification column includes a sealing cap movably mounted on the top of the column. A conduit is fixedly connected to the top of the sealing cap. An adjustment component is provided inside the conduit. The adjustment component includes a sphere movably mounted inside the conduit. A flow port with the same diameter as the inner hole of the conduit is opened in the center of the sphere. A connecting rod with its outer end movably penetrating the side wall of the conduit and fixedly connected to an adjustment knob is fixed to one side of the surface of the sphere. The connecting rod is used to drive the sphere to rotate inside the conduit, thereby changing the cross-sectional area of ​​the conduit channel.

[0008] A locking block is movably installed on the surface of the connecting rod, and the locking block is locked and fixed to the guide tube. A limiting block for limiting the rotation angle of the connecting rod is fixed to the surface of the connecting rod.

[0009] By adopting the above technical solution, the cross-section of the conduit gradually decreases as the adjustment knob is turned, thus slowing down the flow rate of the fluid. This allows for adjustment of the flow rate when the sample enters the purification tube, preventing excessive flow rate from generating bubbles and affecting the purification effect.

[0010] Preferably, the surface of the connecting rod is provided with a groove that matches the shape of the locking block, the locking block is located in the groove and slides longitudinally, the locking block is spherical, and a spring with its bottom end fixed to the bottom of the groove is fixed to the bottom of the locking block. The surface of the guide tube is provided with a perforation that matches the shape of the connecting rod, and the inner wall of the perforation is provided with a plurality of slots that match the shape of the locking block, and the plurality of slots are arranged in a ring array.

[0011] By adopting the above technical solution, the angle positioning is achieved by the mechanical cooperation between the card block and the card slot. Since the card slots are arranged in a circular array, the angular intervals between them are uniform. Whenever the card block is engaged in a card slot, the connecting rod rotates by a fixed angle. This fixed angle can be precisely determined by designing the number of card slots and the intervals between them. Therefore, by calculating the number of card blocks engaged in card slots, we can accurately know how many angles the connecting rod has rotated. This design is not only simple and effective, but also has high precision and stability.

[0012] Preferably, the sidewall of the catheter is provided with a rotating groove that matches the shape of the limiting block, and the limiting block is located in the rotating groove and can only rotate 90 degrees clockwise.

[0013] By adopting the above technical solution, the flow port of the ball is originally aligned with or nearly aligned with the axis of the conduit, at which point the conduit cross-section is at its maximum. When the connecting rod is rotated 90 degrees, the flow port of the ball becomes perpendicular or nearly perpendicular to the axis of the conduit, at which point the conduit is completely blocked. The ball only needs to be rotated clockwise 0-90 degrees to adjust the conduit cross-section from its maximum to its minimum.

[0014] Preferably, a packing layer is provided in the middle of the column, and a drain pipe is connected to the bottom of the column.

[0015] By employing the above-described technical solution, the packing layer utilizes specific materials that selectively interact with exosome surface markers, thereby achieving effective exosome capture and separating exosomes from complex biological samples (such as blood, urine, and cell culture supernatants). The packing layer not only captures exosomes but also removes impurities from the sample, such as proteins, cell debris, and other non-exosome particles, through its structural characteristics and material properties. This process contributes to obtaining high-purity, high-quality exosomes. Due to the exosome capture effect of the packing layer, exosomes in the sample are concentrated on the packing layer. This allows for the use of exosomes at higher concentrations in subsequent experiments or applications, thereby improving experimental sensitivity and accuracy. High-quality packing layer materials can maximize the preservation of exosome integrity and bioactivity during capture and purification. This is crucial for subsequent exosome analysis, functional studies, and applications.

[0016] Preferably, filter plates are provided at both ends of the packing layer, and the filter plates are provided with filter membranes for removing impurities from the sample.

[0017] By employing the above technical solution, the filter plate, acting as the first line of defense, effectively blocks and removes larger particles and impurities from the sample, such as cell debris and protein aggregates. This helps reduce the burden on subsequent purification steps and improves overall purification efficiency. The filter membrane, located inside the filter plate, has a carefully designed pore size to further filter out smaller impurities and particles, including small molecules that may interfere with exosome purity and function. This ensures that the final exosomes have high purity and good biological activity. The combined design of the filter plate and filter membrane not only helps remove impurities but also effectively protects the packing layer inside the purification column. By preventing large particles and impurities from entering the packing layer, the lifespan of the packing layer is extended, reducing the decrease in purification efficiency caused by clogging. Through layer-by-layer filtration, this design significantly improves the purification quality of exosomes.

[0018] A clamp for an exosome biopurification column as described above includes a three-jaw clamp for holding and fixing the column. The three-jaw clamp is fixedly mounted on an iron frame via double set screws. The three-jaw clamp includes three jaws, and each of the three jaws is fixedly fitted with an air bladder.

[0019] By adopting the above technical solution, the inflatable bladder can provide a more uniform and stable clamping force, helping to ensure that the purification column remains stable during experiments and does not shake or shift. The flexibility of the bladder can reduce the pressure on the surface of the purification column during clamping, reducing the risk of clamp marks or damage, thereby better protecting the integrity of the purification column.

[0020] Preferably, a connecting tube is fixedly connected to the surface of the airbag, and an inflation ball for inflating the airbag is fixedly connected to the end of the connecting tube away from the airbag. An inflation valve is fixedly connected to the connecting tube.

[0021] By adopting the above technical solution, the clamping force can be easily and quickly adjusted by using an inflatable ball to inflate the airbag, which simplifies the experimental operation process and improves work efficiency.

[0022] Preferably, the surface of the airbag abuts against the surface of the column after inflation.

[0023] By adopting the above technical solution, the friction between the air bladder and the column is increased, ensuring that the purification column remains stable during the experiment and does not shake or shift.

[0024] In summary, this application includes at least one of the following beneficial technical effects:

[0025] 1. By rotating the adjustment knob, the ball rotates, gradually reducing the cross-section of the conduit. This slows down the fluid flow. When the ball rotates to a position where the flow port is perpendicular or nearly perpendicular to the conduit axis, the conduit is completely blocked. This adjusts the flow rate of the sample entering the purification tube, preventing excessive flow and air bubbles that could affect purification. The mechanical engagement between the locking blocks and slots achieves angle positioning. Since the slots are arranged in a circular array, the angular intervals between them are uniform. Each time a locking block engages a slot, the connecting rod rotates by a fixed angle. Therefore, by calculating the number of slots engaged, we can accurately determine the angle by which the connecting rod has rotated. This design is not only simple and effective but also boasts high precision and stability.

[0026] 2. The inflatable bladder provides a more uniform and stable clamping force, helping to ensure the purification column remains stable during experiments, preventing shaking or displacement. The flexibility of the bladder can reduce pressure on the surface of the purification column during clamping, lowering the risk of clamp marks or damage, thus better protecting the integrity of the purification column. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the overall structure of this application;

[0028] Figure 2 This is a schematic diagram of the internal structure of the column in this application;

[0029] Figure 3 This is a schematic diagram of the adjustment component structure of this application;

[0030] Figure 4 This is a schematic cross-sectional view of the adjustment component in this application;

[0031] Figure 5 for Figure 4 Enlarged schematic diagram of the structure at point A in the middle;

[0032] Figure 6 This is a schematic diagram of the limiting block and the rotating groove structure of this application;

[0033] Figure 7 This is a schematic diagram of the three-jaw clamp and airbag structure of this application;

[0034] Figure 8 This is a schematic diagram of the airbag structure of this application.

[0035] Attached reference numerals: 1. Iron frame; 11. Column; 2. Double-top screw; 21. Clamping block one; 22. Clamping block two; 23. Bolt one; 24. Bolt two; 3. Three-jaw clamp; 31. Jaw; 32. Connecting column;

[0036] 41. Column; 42. Sealing cap; 43. Conduit; 44. Connector; 45. Adjustment assembly;

[0037] 451. Sphere; 452. Flow port; 453. Connecting rod; 454. Adjusting knob; 455. Locking block; 456. Slide groove; 457. Spring; 458. Locking groove; 459. Limiting block; 4510. Rotary groove;

[0038] 46. ​​Drain pipe; 47. Packing layer; 48. Filter plate; 49. Filter membrane; 410. Perforation; 411. Cavity;

[0039] 5. Airbag; 52. Connecting tube; 53. Inflatable ball; 54. Inflation valve. Detailed Implementation

[0040] The following is in conjunction with the appendix Figures 1-8 This application will be described in further detail.

[0041] This application discloses an exosome biopurification column and fixture.

[0042] Example 1

[0043] Reference Figures 1-2 An exosome biopurification column includes a sealing cap 42 movably mounted on the top of a column body 41, a conduit 43 fixedly connected to the center of the top of the sealing cap 42 with its bottom end fixedly penetrating the side wall of the sealing cap 42 and extending into the column body 41, a connector 44 fixedly connected to the top of the conduit 43, and a drain pipe 46 fixedly connected to the center of the bottom of the column body 41. A filter plate 48, a packing layer 47, and another filter plate 48 are arranged sequentially from top to bottom in the center of the inner cavity of the column body 41. The two filter plates 48 abut against the two ends of the packing layer 47 on the side closest to the packing layer 47, respectively. A filter membrane 49 is disposed inside the filter plate 48. The filter plate 48 is used to block and remove larger particles and impurities in the sample, such as cell debris and protein aggregates. The pore size of the filter membrane 49 is carefully designed to further filter out smaller impurities and particles.

[0044] First, a sample containing exosomes (such as cell culture supernatant, blood sample, etc.) is loaded onto the top of column 41 through connector 44. The sample flows through packing layer 47 within column 41. Packing layer 47 of column 41 is typically designed with specific chemical or physical properties to interact with exosomes. These interactions may be based on the surface charge, size, shape, or other biophysical characteristics of the exosomes. As the sample passes through packing layer 47, the exosomes are captured on it, while most other impurities (such as proteins, nucleic acids, small molecules, etc.) continue to flow and pass through the column, thus achieving initial separation of exosomes from impurities.

[0045] To remove non-specifically bound material remaining in column 41, the purification column is typically washed with one or more wash buffers. The composition and conditions of these wash buffers (such as pH, ionic strength, etc.) are optimized to ensure effective removal of impurities without damaging the captured exosomes.

[0046] Finally, the captured exosomes are eluted from the purification column using an appropriate elution buffer. The composition and conditions of the elution buffer are also optimized to ensure efficient and complete recovery of the exosomes. The eluted exosome solution is typically further processed, such as concentrated, purified, or used for subsequent experimental analysis.

[0047] It is important to note that different exosome purification columns may employ different purification principles and mechanisms, therefore the specific purification steps and conditions may vary. Furthermore, strict control of experimental conditions, such as temperature, time, pH of the buffer solution, and ionic strength, is necessary during purification to ensure effective purification and the bioactivity of the exosomes.

[0048] Reference Figure 3 An adjusting component 45 is disposed in the center inside the conduit 43. The adjusting component 45 includes a ball 451 movably installed inside the conduit 43. A cavity 411 adapted to the shape of the ball 451 is formed in the center of the inner wall of the conduit 43 channel. The ball 451 is located in the cavity 411 and can rotate. A flow port 452 with the same diameter as the conduit 43 channel is formed in the center inside the ball 451. The flow port 452 is initially connected to the conduit 43 channel. The direction of the axis of the conduit 43 is consistent or nearly consistent. The connecting rod 453 is fixed to one side of the surface of the sphere 451. The conduit 43 has a perforation 410 at the center of one side of the sphere 451 that communicates with the cavity 411. The connecting rod 453 is located inside the perforation 410 and the surface of the connecting rod 453 abuts against the inner wall of the perforation 410. One end of the connecting rod 453 is fixed to the center of one side of the surface of the sphere 451, and the end of the connecting rod 453 away from the sphere 451 is fixed to an adjustment knob 454.

[0049] Initially, the flow port 452 of the sphere 451 is aligned with or nearly aligned with the axis of the conduit 43. At this point, the cross-sectional area of ​​the conduit 43 is at its maximum, resulting in a faster fluid flow rate. Then, rotating the adjustment knob 454 causes the sphere 451 to rotate clockwise, gradually reducing the cross-sectional area of ​​the conduit 43. This slows down the fluid flow rate. When the sphere 451 rotates until the flow port 452 is perpendicular or nearly perpendicular to the axis of the conduit 43, the conduit 43 is completely blocked. This allows adjustment of the sample flow rate into the column 41, preventing excessive flow from generating bubbles and affecting the purification effect.

[0050] Reference Figure 4 , Figure 5A groove 456 is formed on the top surface of the connecting rod 453. A spherical locking block 455 is located inside the groove 456, and the diameter of the groove 456 is equal to the diameter of the locking block 455. The height of the groove 456 is greater than the diameter of the locking block 455, so that the ball 451 can slide longitudinally inside the groove 456. A spring 457 is fixedly connected to the bottom surface of the locking block 455. The end of the spring 457 away from the locking block 455 is fixedly connected to the bottom of the inner cavity of the groove 456. Several locking slots 458 arranged in a ring array are formed on the inner wall of the perforation 410. The number of locking slots 458 and the spacing between them can be designed according to actual needs. The angle between the locking slots 458 at both ends is 90 degrees, and the shape of the locking slots 458 is adapted to the locking block 455.

[0051] The angle positioning is achieved by the mechanical cooperation between the locking block 455 and the locking slot 458. Each locking slot 458 represents a specific rotation angle, and the locking block 455 serves as the angle positioning element. When the connecting rod 453 rotates, the locking block 455 is subjected to a component force in the direction of the slide groove 456, and then the locking block 455 slides into the slide groove 456. The locking block 455 will rotate together with the connecting rod 453. When it rotates to a position aligned with a certain locking slot 458, due to the matching shape of the locking block 455 and the locking slot 458, the locking block 455 will be naturally locked by the locking slot 458 under the action of the spring 457, thereby achieving the angle positioning.

[0052] Because the slots 458 are arranged in a circular array, the angular intervals between them are uniform. Each time a card block 455 engages a slot 458, the connecting rod 453 rotates by a fixed angle. This fixed angle can be precisely determined by designing the number of slots 458 and their intervals. Therefore, by calculating the number of slots 455 engage, we can accurately know the angle by which the connecting rod 453 rotates, thus precisely controlling the rotation angle of the sphere 451. This allows for precise adjustment of the cross-sectional area of ​​the conduit 43, and consequently, precise control of the sample flow rate into the column 41, preventing excessive flow rates from generating bubbles and affecting the purification effect.

[0053] Reference Figure 6 The limiting block 459 is fixed to the top center of the surface of the connecting rod 453, and the limiting block 459 is arranged parallel to the locking block 455. The rotating groove 4510 is opened on the side wall of the guide tube 43. The limiting block 459 is located in the rotating groove 4510, and the top and both sides of the limiting block 459 abut against the inner wall of the rotating groove 4510. The A end of the limiting block 459 abuts against one end of the inner cavity of the rotating groove 4510. When the limiting block 459 rotates 90 degrees clockwise in the rotating groove 4510, the B end of the limiting block 459 abuts against the other end of the inner cavity of the rotating groove 4510.

[0054] The limiting block 459 is fixed on the connecting rod 453, and its shape is adapted to the shape of the rotating groove 4510 to ensure that the limiting block 459 can move smoothly within the rotating groove 4510. Due to the shape limitation of the rotating groove 4510, the limiting block 459 can only rotate clockwise to a position of 90 degrees. Once the limiting block 459 reaches this position, the shape or wall of the rotating groove 4510 will prevent it from continuing to rotate, thereby achieving precise control of the rotation angle of the connecting rod 453. Originally, the flow port 452 of the ball 451 and the axis of the conduit 43 are aligned or nearly aligned, and the cross-section of the conduit 43 is at its maximum at this time. When the connecting rod 453 rotates 90 degrees, the flow port 452 of the ball 451 is perpendicular or nearly perpendicular to the axis of the conduit 43. At this time, the conduit 43 is completely blocked. Then, the ball 451 only needs to rotate clockwise 0-90 degrees to adjust the cross-section of the conduit 43 from the maximum to the minimum.

[0055] The implementation principle of the exosome biopurification column in this application embodiment is as follows: Rotating the adjusting knob 454 clockwise causes the connecting rod 453 to rotate the ball 451 clockwise. When the connecting rod 453 rotates, the locking block 455 receives a component force in the direction of the slide groove 456, causing the locking block 455 to slide into the slide groove 456. The locking block 455 then rotates together with the connecting rod 453. When it rotates to a position aligned with a certain slot 458, the locking block 455 will naturally be locked into the slot 458 under the action of the spring 457. The locking mechanism allows for angle positioning. Each time the locking block 455 engages with a slot 458, the connecting rod 453 rotates by a fixed angle. Therefore, by calculating the number of times the locking block 455 engages with slot 458, we can accurately determine the angle by which the connecting rod 453 has rotated. This allows for precise control of the rotation angle of the sphere 451, which in turn allows for precise adjustment of the cross-sectional area of ​​the conduit 43. Consequently, we can precisely control the flow rate of the sample entering the column 41, preventing excessive flow rate from generating bubbles and affecting the purification effect.

[0056] The limiting block 459 restricts the connecting rod 453 to only rotate the ball 451 clockwise by 90 degrees. Originally, the flow port 452 of the ball 451 is aligned with or nearly aligned with the axis of the conduit 43. At this time, the cross-section of the conduit 43 is at its maximum. After the connecting rod 453 rotates 90 degrees, the flow port 452 of the ball 451 is perpendicular to or nearly perpendicular to the axis of the conduit 43. At this time, the conduit 43 is completely blocked. Then, the ball 451 only needs to rotate clockwise from 0 to 90 degrees to adjust the cross-section of the conduit 43 from its maximum to its minimum.

[0057] Example 2

[0058] Reference Figure 7 , Figure 8A clamp for an exosome biopurification column, comprising a three-jaw clamp 3 for clamping and fixing the column body 41, an iron frame 1 comprising a column 11, the column 11 being located within a clamping block 21 of a double-top screw 2, and the surface of the column 11 abutting against the inner wall of the clamping block 21, a bolt 23 threadedly connected to the clamping block 21 being rotated so that one end of the bolt 23 near the column 11 abuts against the surface of the column 11, a connecting column 32 of the three-jaw clamp 3 being located within a clamping block 22, and the surface of the connecting column 32 abutting against the inner wall of the clamping block 22, a bolt 24 threadedly connected to the clamping block 22 being rotated so that one end of the bolt 24 near the connecting column 32 abuts against the surface of the connecting column 32, thereby fixing the connecting column 32 within the clamping block 22, the three-jaw clamp 3 comprising three jaws 31, and an airbag 5 having three jaws and being respectively fixedly sleeved on the surface of the three jaws 31;

[0059] Three connecting tubes 52 are fixed to the surfaces of three airbags 5 respectively. An inflatable ball 53 is fixed to the end of the connecting tube 52 away from the airbag 5. Pressing the inflatable ball 53 is used to inflate the airbag 5. Three inflation valves 54 are fixed to the surfaces of the three connecting tubes 52 respectively. The surface of the column 41 abuts against the surface of the inflated airbag 5.

[0060] By squeezing the inflation ball 53, the air bladder 5 is inflated. The surface of the air bladder 5 abuts against the surface of the column 41, clamping and fixing the column 41. The inflated air bladder 5 can provide a more uniform and stable clamping force, which helps to ensure that the purification column remains stable during the experiment and does not shake or shift. The softness of the air bladder 5 can reduce the pressure on the surface of the column 41 during clamping to a certain extent, reducing the risk of clamp marks or damage, thereby better protecting the integrity of the column 41.

[0061] The implementation principle of a clamp in this application embodiment is as follows: by squeezing the inflation ball 53, the inflation ball 53 inflates the airbag 5, and the surface of the airbag 5 abuts against the surface of the column 41 to clamp and fix the column 41.

[0062] The above are merely optional embodiments of this disclosure and are not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure.

Claims

1. An exosome biopurification column, comprising a sealing cover (42) movably installed on the top of a column body (41), a conduit (43) is fixed on the top of the sealing cover (42), and an adjusting assembly (45) is arranged in the conduit (43), characterized in that: The adjusting assembly (45) comprises a ball (451) movably mounted in the conduit (43), a flow passage (452) is formed in the center of the ball (451) and has the same diameter as the inner hole of the conduit (43), one side of the surface of the ball (451) is fixedly connected with a connecting rod (453) movably penetrating through the side wall of the conduit (43) and fixedly connected with an adjusting knob (454), and the connecting rod (453) is used to drive the ball (451) to rotate in the conduit (43) so as to change the passage sectional area of the conduit (43); A clamping block (455) is movably mounted on the surface of the connecting rod (453), the clamping block (455) is fixedly connected with the conduit (43), and a limiting block (459) for limiting the rotating angle of the connecting rod (453) is fixedly connected with the surface of the connecting rod (453).

2. The exosome biopurification column of claim 1, wherein: A sliding groove (456) matched with the shape of the clamping block (455) is formed in the surface of the connecting rod (453), the clamping block (455) is located in the sliding groove (456) and longitudinally slides, the clamping block (455) is spherical, a spring (457) is fixedly connected with the bottom of the clamping block (455) and fixedly connected with the bottom of the inner cavity of the sliding groove (456), a perforation (410) matched with the shape of the connecting rod (453) is formed in the surface of the conduit (43), a plurality of clamping grooves (458) matched with the shape of the clamping block (455) are formed in the inner wall of the perforation (410), and the plurality of clamping grooves (458) are arranged in an annular array.

3. The exosome biopurification column of claim 2, wherein: A rotating groove (4510) matched with the shape of the limiting block (459) is formed in the side wall of the conduit (43), and the limiting block (459) is located in the rotating groove (4510) and only rotates 90 degrees clockwise.

4. The exosome biopurification column of claim 1, wherein: A filler layer (47) is arranged in the middle of the column body (41), and a drainage pipe (46) is in communication with the bottom of the column body (41).

5. The exosome biopurification column of claim 4, wherein: Filter plates (48) are arranged at both ends of the filler layer (47), and filter membranes (49) for removing impurities in samples are arranged in the filter plates (48).

6. A clamp for use with an exosome biopurification column according to claim 5, characterized in that: A three-jaw clamp (3) for clamping and fixing the column body (41) is arranged, the three-jaw clamp (3) is fixedly mounted on the iron stand (1) through double jackscrews (2), and the three-jaw clamp (3) comprises three clamping jaws (31), and air bags (5) are fixedly arranged on the surfaces of the three clamping jaws (31).

7. A clamp according to claim 6, wherein: A connecting pipe (52) is fixedly connected with the surface of the air bag (5), an inflating ball (53) for inflating the air bag (5) is fixedly connected with one end of the connecting pipe (52) away from the air bag (5), and an inflating valve (54) is fixedly connected with the connecting pipe (52).

8. A clamp according to claim 7, wherein: After the air bag (5) is inflated, the surface of the air bag (5) abuts against the surface of the column body (41).

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